CN111352225A - Auxiliary device and method for correcting scanning microscopic imaging angle - Google Patents
Auxiliary device and method for correcting scanning microscopic imaging angle Download PDFInfo
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- CN111352225A CN111352225A CN202010276984.1A CN202010276984A CN111352225A CN 111352225 A CN111352225 A CN 111352225A CN 202010276984 A CN202010276984 A CN 202010276984A CN 111352225 A CN111352225 A CN 111352225A
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/362—Mechanical details, e.g. mountings for the camera or image sensor, housings
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/10—Scanning
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/12—Circuits of general importance; Signal processing
- G01N2201/127—Calibration; base line adjustment; drift compensation
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Abstract
The invention discloses an auxiliary device and method for correcting a scanning microscopic imaging angle. The invention comprises an auxiliary adjusting block, a mounting bracket and an auxiliary adjusting strip block; the auxiliary adjusting block is provided with a through hole and a threaded hole; the inner hexagonal screw penetrates through the through hole and then is connected with a threaded hole on the scanning camera; the mounting bracket is integrally formed by an upper end panel, an L-shaped bearing plate and a lower end bottom plate; the upper end panel is connected with the lower end bottom plate through an L-shaped bearing plate; the auxiliary adjusting strip block is arranged on the side face of the lower end bottom plate, a pair of through holes and threaded holes are formed in the auxiliary adjusting block, and the inner hexagonal screw penetrates through the through holes to be matched with the threaded holes in the side face of the lower end bottom plate of the mounting support. The invention is suitable for the microscopic imaging system of most digital slice scanning devices.
Description
Technical Field
The invention relates to the technical field of scanning microscopic imaging. In particular to an auxiliary device and a method for correcting a scanning microscopic imaging angle. The method is used in the process of installing and debugging the equipment, so that the high-quality imaging can be quickly and efficiently kept after the adjustment.
Background
In a microscope, particularly a scanning microscope, when a slice, an objective lens, a tube lens, a scanning camera and the like are installed, the angle of a microscope imaging system has deviation due to installation errors and the like, and the installation of the mechanisms needs to be adjusted to correct the deviation, so that the scanning imaging effect and quality are ensured.
Manual differential head and spring mechanism are commonly used to present scanning microscope angle of adjustment's mode, combine the lever to come angle regulation, and this kind of mechanism simple structure does not have the auto-lock ability yet to can not keep current state for a long time, reuse needs readjustment, and the drawback is obvious.
In order to solve the existing problems, the manual differential head is replaced by a fine-tooth screw, the spring is replaced by a fine-tooth fastening screw, and meanwhile, the lever structure is adjusted, so that the driving end of the lever is longer, and the adjustment precision of the driven end is ensured.
Disclosure of Invention
The invention aims to innovatively improve the design and installation method of digital slice scanning equipment, and provides an auxiliary device and method for correcting a scanning microscopic imaging angle and an adjusting method which is quick and simple to adjust and high in stability after being adjusted.
The technical scheme adopted by the invention for solving the technical problems is as follows:
an auxiliary device for correcting a scanning microscopic imaging angle comprises an auxiliary adjusting block (3) of a shooting device, a mounting bracket (4) of a whole set of scanning imaging device and an auxiliary adjusting block (5) of the scanning imaging device;
the auxiliary adjusting block (3) is provided with a through hole (301) and a threaded hole (302); the inner hexagonal screw passes through the through hole (301) and then is connected with a threaded hole on a scanning camera (203) in the microscopic imaging device (2); mounting a set screw at the threaded hole (302) for supporting a scanning camera; according to the matching of the hexagon socket head cap screw at the through hole (301) and the fastening screw at the threaded hole (302), the posture of the camera can be adjusted, so that the scanning camera can be located at a reliable position;
the lateral section of the auxiliary adjusting block is L-shaped; the positive section is in a convex shape; the through hole (301) and the threaded hole (302) are arranged in the middle of the L-shaped lug (303); two threaded holes (302) are arranged between the two through holes (301).
The mounting bracket (4) is integrally formed by an upper end panel (404), an L-shaped bearing plate (405) and a lower end bottom plate (406); the upper end panel (404) is connected with the lower end bottom plate (406) through an L-shaped bearing plate (405);
wherein the upper end panel (404) of the mounting bracket (4) is arranged on the outer side wall of the micro-imaging device (2); the upper end panel (404) is provided with 4 pairs of straight through holes (401) and threaded holes (402); the inner hexagonal screw penetrates through the through hole (401) to be matched with a threaded hole in the outer side wall of the microscopic imaging device (2); mounting a set screw at the threaded hole (402) for supporting the microscopic imaging device; according to the matching of the hexagon socket head cap screw at the through hole (401) and the fastening screw at the threaded hole (402), the posture of the microscopic imaging device can be adjusted, so that the microscopic imaging device can be positioned at a reliable position;
the right section of the upper end panel (404) is rectangular, and a pair of through holes (401) and threaded holes (402) are respectively formed in four right-angle ends of the rectangle; the threaded hole (402) is arranged on the inner side of the through hole (401), and the through hole (401) and the threaded hole (402) are arranged on a right-angled 45-degree angular line;
the auxiliary adjusting block (5) is arranged on the side surface of the lower end bottom plate (406), a pair of through holes (501) and threaded holes (502) are arranged on the auxiliary adjusting block (5),
the inner hexagonal screw passes through the through hole (501) and is matched with a side threaded hole of a lower end base plate (406) of the mounting bracket (2); a fastening screw is arranged at the threaded hole (502) and used for supporting the mounting bracket (2); according to the matching of the hexagon socket head cap screw at the through hole (501) and the fastening screw at the threaded hole (502), the posture of the mounting bracket (2) can be adjusted, so that the microscopic imaging device can be positioned at a reliable position;
a pair of through holes (501) and threaded holes (502) on the auxiliary adjusting bar block (5) are respectively arranged at two symmetrical ends.
Further, the bottom side 304 of the auxiliary adjusting block is connected with the micro-imaging device (2);
furthermore, the inner corners of the upper end panel (404) connected with the lower end bottom plate (406) through the L-shaped bearing plate (405) are all arc-shaped; and the inner corner of the L-shaped bearing plate (405) is also arc-shaped. And the inner hexagonal screws in the auxiliary adjusting block (3) and the mounting bracket (4) are M3 fine-tooth screws. The inner hexagonal screw in the auxiliary adjusting bar block (5) is an M4 fine-tooth screw;
an auxiliary method for correcting a scanning microscopic imaging angle is specifically realized as follows:
posture adjustment of the scanning camera:
the auxiliary adjusting block (3) can adjust the angle of the camera in the Z-axis rotation direction, the screw pitch of the screw is 0.35mm according to the specification table of M3 fine-thread screws, the screw rotation angle of one side is α 1, the locking distance of the side is a1,
a1=(α1/360*0.35),
combining the radius r1 of the rotation of the motor with the radius of 15mm, the current rotation angle can be calculated to be β 1:
from the angular deviation of the image captured by the camera, then combined with the formula of the rotation angle, the solution for the angle α 11 of the required adjustment screw is as follows:
β 11, adjusting the rotation angle of the screw to a proper position, locking the corresponding screw and the fastening screw, and finishing the posture adjustment of the camera;
posture adjustment of the scanning imaging device:
the mounting bracket (4) can adjust the angle of the scanning imaging device in the Y-axis rotation direction, the screw pitch of the screw is 0.35mm according to the specification table of M3 fine-thread screws, the screw rotation angle of one side is α 2, the locking distance of the side is a2,
a2=(α2/360*0.35),
combining the radius r2 of the rotation to 26mm, the current rotation angle can be calculated to be β 2:
from the angular deviation of the image captured by the camera, then combined with the formula of the rotation angle, the solution of the angle α 21 of the required adjustment screw is as follows:
β 21, adjusting the screw rotation angle to the proper position, locking the corresponding screw and the fastening screw, and finishing the posture adjustment of the scanning imaging device;
and (3) correcting the imaging angle of the whole scanning microscopic imaging shooting device:
after the whole scanning and shooting device assembly is adjusted by an auxiliary adjusting block (3) of the shooting device and a mounting bracket (4) of the whole set of scanning and imaging device, the whole scanning and shooting device assembly needs to be mounted on a reference surface; the reference surface installation of the whole assembly is adjusted by an auxiliary adjusting strip (5) of the scanning imaging device, and the method comprises the following specific steps:
the scanning microscopic imaging shooting device is fixed by adjusting two pairs of M4 fine-tooth inner hexagonal screws and M4 fine-tooth fastening screws at the straight through hole (501) and the threaded hole (502) to a desired position and then locking the inner hexagonal screws arranged at the lower end bottom plate (403). Thus, the imaging angle correction of the whole scanning microscopic imaging shooting device is completed.
The adjustment to the expected position is specifically adjusted as follows:
according to specification tables of M4 fine thread hexagon socket head cap screw and M4 fine thread fastening screw, the screw pitch is 0.50mm, if the screw rotation angle on one side is α 3, the locking distance on the side is a3,
a3=(α3/360*0.5),
combining the radius r3 of the rotation of the motor with the radius r3 of 80mm, the current rotation angle can be calculated to be β 3:
from the angular deviation of the image captured by the camera, then combined with the formula of the rotation angle, the solution of the angle α 31 of the required adjustment screw is as follows:
β 31, adjusting the screw angle to proper position, locking the corresponding screw and the tightening screw, and finishing the adjustment.
The invention has the following beneficial effects:
according to the invention, through the mutual matching of the fine-tooth screw and the fine-tooth fastening screw, the imaging angle can be stably adjusted within a very small adjusting range according to the lever principle, and the state can be stably maintained, so that the purpose of adjusting the imaging angle is achieved. The image can be accurately corrected in angle according to the angle deviation of the image and a calculation formula, and the device is suitable for a digital slice scanner, but the method is suitable for a microscopic imaging system of most digital slice scanners.
Drawings
FIG. 1 is a flow chart of a method for mounting a scanning microscopic imaging angle correction auxiliary device according to the present invention;
FIG. 2 is a mounting structure of a micro-imaging device according to the present invention;
FIG. 3 is a view showing a structure of a camera attitude adjustment assisting section according to the present invention;
FIG. 4 is a schematic view of a scanning imaging camera assembly according to the present invention;
FIG. 5 is a schematic diagram of a scanning imaging camera assembly according to the present invention.
Detailed Description
The following detailed description of the novel auxiliary device will be made with reference to the accompanying drawings and the embodiments
The invention relates to the technical field of scanning microscopic imaging. In particular to an auxiliary device and a method for correcting a scanning microscopic imaging angle. The method is used in the process of installing and debugging the equipment, so that the high-quality imaging can be quickly and efficiently kept after the adjustment.
As shown in fig. 1, the installation operation of an auxiliary device for scanning microscopic imaging angle correction is as follows:
firstly, the tube lens is installed in place, and the tube lens can be directly installed and locked by using a set screw after being installed in place according to the matching of the contact surface of the tube lens and the lens installing support 4.
Then, the scanning camera 203 and the zoom lens 202 are assembled, and the interface types of the two are consistent, which is a "C" interface, and the two can be directly screwed to complete the assembly. It should be noted here that the lens and the scanning camera can be selected according to the actual use condition, and the innovative method is not only suitable for the lens and the camera, but also suitable for other types of lenses and cameras.
Then, the mounting end of the zoom lens 203 is connected and matched with the corresponding mounting part of the auxiliary adjusting block 3, the tightening screw at the moment needs to be temporarily locked, and the tightening screw needs to be loosened when the posture of the scanning camera is subsequently adjusted.
Then, the camera attitude adjusting device is mounted, and is firstly mounted on the lens mounting bracket 4 to be fixed, so that the position and attitude of the scanning camera can be adjusted conveniently in the following process.
Then, the microscopic imaging device is installed on the installation support 4, screws are locked in advance on the same principle, the microscopic imaging device is guaranteed not to fall off, and the position and the posture of the microscopic imaging device can be adjusted subsequently.
Then, the equipment assembly is installed on a reference surface, the posture is adjusted through the auxiliary adjusting bar 5, and then the scanning objective lens is installed after the equipment assembly is fixed.
And finally, after all the components are installed, the adjustment is carried out by means of an auxiliary device according to the use condition of the whole set of equipment. In the adjusting process, the screws and the fastening screws are fastened and loosened to form matching, so that the parts needing to be adjusted are adjusted, and the purpose is achieved. The fine thread can be used to make the adjusting process more stable and the result more accurate.
The specific adjustment sequence and operation method are as follows:
firstly, after the whole scanning and shooting device assembly is well adjusted through an auxiliary adjusting block (3) of the shooting device and a mounting bracket (4) of the whole set of scanning and imaging device, the whole scanning and shooting device assembly needs to be mounted on a reference surface; the reference plane mounting of the entire assembly is adjusted by scanning the auxiliary adjustment bar (5) of the imaging device so that the X-direction of the assembly is parallel to the X-axis motion direction of the slices.
The method comprises the following specific steps:
after the two pairs of M4 fine-tooth inner hexagonal screws and M4 fine-tooth fastening screws at the straight through hole (501) and the threaded hole (502) are matched in a screwing and loosening manner and adjusted to the expected positions, the inner hexagonal screws arranged at the lower end bottom plate (403) are locked, and the scanning microscopic imaging shooting device is fixed. Thus, the imaging angle correction of the whole scanning microscopic imaging shooting device is completed.
And secondly, adjusting the position posture of the microscopic imaging device to enable the Z-axis direction of the device to be vertical to the upper surface of the slice, and uniformly locking the inner hexagonal screws after adjusting the two pairs of M3 fine-tooth inner hexagonal screws and M3 fine-tooth fastening screws at the straight through hole (401) and the threaded hole (402) to be in fit with each other by screwing and loosening, so that the verticality angle of the whole scanning microscopic imaging device and the upper surface of the slice is corrected.
And finally, adjusting the posture of the camera, wherein the posture of the camera is adjusted by matching the screwing and loosening of two pairs of M3 hexagon socket head cap screws and M3 fine tooth fastening screws at the straight through hole (301) and the threaded hole (302) in the same principle. The stability of the tightening screw for tightening the zoom lens is ensured.
The adjusting process has reliable adjusting formula reference.
In the above formula, β is the existing angle deviation, r is the turning radius of the device, d is the thread pitch, then if the data reference is made to the turning angles of the screw and the set screw, the calculation can be done according to the above formula, and the adjustment can be made according to the calculation result.
The above-described apparatus description and operation examples are only a preferred embodiment of the present invention, and are not intended to limit the present invention. Various changes may be made by those skilled in the pertinent art without departing from the spirit and scope of the present invention. Therefore, the technical scheme obtained by adopting the mode of equivalent replacement or equivalent transformation is within the protection scope of the invention.
Claims (4)
1. An auxiliary device for correcting a scanning microscopic imaging angle is characterized by comprising an auxiliary adjusting block (3) of a shooting device, a mounting bracket (4) of a whole set of scanning imaging device and an auxiliary adjusting block (5) of the scanning imaging device;
the auxiliary adjusting block (3) is provided with a through hole (301) and a threaded hole (302); the inner hexagonal screw passes through the through hole (301) and then is connected with a threaded hole on a scanning camera (203) in the microscopic imaging device (2); mounting a set screw at the threaded hole (302) for supporting a scanning camera; according to the matching of the hexagon socket head cap screw at the through hole (301) and the fastening screw at the threaded hole (302), the posture of the camera can be adjusted, so that the scanning camera can be located at a reliable position;
the lateral section of the auxiliary adjusting block is L-shaped; the positive section is in a convex shape; the through hole (301) and the threaded hole (302) are arranged in the middle of the L-shaped lug (303); the two threaded holes (302) are arranged between the two through holes (301);
the mounting bracket (4) is integrally formed by an upper end panel (404), an L-shaped bearing plate (405) and a lower end bottom plate (406); the upper end panel (404) is connected with the lower end bottom plate (406) through an L-shaped bearing plate (405);
wherein the upper end panel (404) of the mounting bracket (4) is arranged on the outer side wall of the micro-imaging device (2); the upper end panel (404) is provided with 4 pairs of straight through holes (401) and threaded holes (402); the inner hexagonal screw penetrates through the through hole (401) to be matched with a threaded hole in the outer side wall of the microscopic imaging device (2); mounting a set screw at the threaded hole (402) for supporting the microscopic imaging device; according to the matching of the hexagon socket head cap screw at the through hole (401) and the fastening screw at the threaded hole (402), the posture of the microscopic imaging device can be adjusted, so that the microscopic imaging device can be positioned at a reliable position;
the right section of the upper end panel (404) is rectangular, and a pair of through holes (401) and threaded holes (402) are respectively formed in four right-angle ends of the rectangle; the threaded hole (402) is arranged on the inner side of the through hole (401), and the through hole (401) and the threaded hole (402) are arranged on a right-angled 45-degree angular line;
the auxiliary adjusting block (5) is arranged on the side surface of the lower end bottom plate (406), a pair of through holes (501) and threaded holes (502) are arranged on the auxiliary adjusting block (5),
the inner hexagonal screw passes through the through hole (501) and is matched with a side threaded hole of a lower end base plate (406) of the mounting bracket (2); a fastening screw is arranged at the threaded hole (502) and used for supporting the mounting bracket (2); according to the matching of the hexagon socket head cap screw at the through hole (501) and the fastening screw at the threaded hole (502), the posture of the mounting bracket (2) can be adjusted, so that the microscopic imaging device can be positioned at a reliable position;
a pair of through holes (501) and threaded holes (502) on the auxiliary adjusting bar block (5) are respectively arranged at two symmetrical ends.
2. The auxiliary device for scanning microscopic imaging angle correction according to claim 1, characterized in that the bottom side 304 of the auxiliary adjustment block is connected with the microscopic imaging device (2).
3. The auxiliary device for correcting the scanning microscopic imaging angle as recited in claim 1 or 2, characterized in that the inner bends of the upper end panel (404) connected with the lower end bottom plate (406) through the L-shaped bearing plate (405) are all arc-shaped; the inner corner of the L-shaped bearing plate (405) is also arc-shaped; the inner hexagonal screws in the auxiliary adjusting block (3) and the mounting bracket (4) are M3 fine-tooth screws; the inner hexagonal screw in the auxiliary adjusting bar (5) is an M4 fine-tooth screw.
4. The auxiliary method for correcting the scanning microscopic imaging angle according to claim 3 is characterized by being implemented as follows:
posture adjustment of the scanning camera:
the auxiliary adjusting block (3) can adjust the angle of the camera in the Z-axis rotation direction, the screw pitch of the screw is 0.35mm according to the specification table of M3 fine-thread screws, the screw rotation angle of one side is α 1, the locking distance of the side is a1,
a1=(α1/360*0.35),
combining the radius r1 of the rotation of the motor with the radius of 15mm, the current rotation angle can be calculated to be β 1:
from the angular deviation of the image captured by the camera, then combined with the formula of the rotation angle, the solution for the angle α 11 of the required adjustment screw is as follows:
β 11, adjusting the rotation angle of the screw to a proper position, locking the corresponding screw and the fastening screw, and finishing the posture adjustment of the camera;
posture adjustment of the scanning imaging device:
the mounting bracket (4) can adjust the angle of the scanning imaging device in the Y-axis rotation direction, the screw pitch of the screw is 0.35mm according to the specification table of M3 fine-thread screws, the screw rotation angle of one side is α 2, the locking distance of the side is a2,
a2=(α2/360*0.35),
combining the radius r2 of the rotation to 26mm, the current rotation angle can be calculated to be β 2:
from the angular deviation of the image captured by the camera, then combined with the formula of the rotation angle, the solution of the angle α 21 of the required adjustment screw is as follows:
β 21, adjusting the screw rotation angle to the proper position, locking the corresponding screw and the fastening screw, and finishing the posture adjustment of the scanning imaging device;
and (3) correcting the imaging angle of the whole scanning microscopic imaging shooting device:
after the whole scanning and shooting device assembly is adjusted by an auxiliary adjusting block (3) of the shooting device and a mounting bracket (4) of the whole set of scanning and imaging device, the whole scanning and shooting device assembly needs to be mounted on a reference surface; the reference surface installation of the whole assembly is adjusted by an auxiliary adjusting strip (5) of the scanning imaging device, and the method comprises the following specific steps:
after two pairs of M4 fine-tooth inner hexagonal screws and M4 fine-tooth fastening screws at the straight through hole (501) and the threaded hole (502) are adjusted to the expected positions, the inner hexagonal screws arranged at the lower end bottom plate (403) are locked, and the scanning microscopic imaging shooting device is fixed; thus, the imaging angle correction of the whole scanning microscopic imaging shooting device is completed;
the adjustment to the expected position is specifically adjusted as follows:
according to specification tables of M4 fine thread hexagon socket head cap screw and M4 fine thread set screw, the thread pitch of the screw is 0.50mm, and if the rotation angle of the screw on one side is α 3, the locking distance on the side is a 3:
a3=(α3/360*0.5),
combining the radius r3 of the rotation of the motor with the radius r3 of 80mm, the current rotation angle can be calculated to be β 3:
from the angular deviation of the image captured by the camera, then combined with the formula of the rotation angle, the solution of the angle α 31 of the required adjustment screw is as follows:
β 31, adjusting the screw angle to proper position, locking the corresponding screw and the tightening screw, and finishing the adjustment.
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