CN111345408A - Emulsifier special for aquatic animals and preparation method and use method thereof - Google Patents

Emulsifier special for aquatic animals and preparation method and use method thereof Download PDF

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CN111345408A
CN111345408A CN202010202352.0A CN202010202352A CN111345408A CN 111345408 A CN111345408 A CN 111345408A CN 202010202352 A CN202010202352 A CN 202010202352A CN 111345408 A CN111345408 A CN 111345408A
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parts
emulsifier
aquatic animals
feed
special
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CN111345408B (en
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焦莉
孙丽华
王勇
丁为国
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Guangzhou Youbaite Feed Sci & Tech Co ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/105Aliphatic or alicyclic compounds
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/142Amino acids; Derivatives thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/142Amino acids; Derivatives thereof
    • A23K20/147Polymeric derivatives, e.g. peptides or proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/158Fatty acids; Fats; Products containing oils or fats
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K40/00Shaping or working-up of animal feeding-stuffs
    • A23K40/10Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K40/00Shaping or working-up of animal feeding-stuffs
    • A23K40/30Shaping or working-up of animal feeding-stuffs by encapsulating; by coating

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Animal Husbandry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Insects & Arthropods (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Birds (AREA)
  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)

Abstract

The invention provides an emulsifier special for aquatic animals, a preparation method and a use method thereof, and the emulsifier comprises the following components in parts by weight: 5-15 parts of sodium caseinate; 24-40 parts of modified phospholipid; 15-30 parts of sucrose fatty acid ester; 4-10 parts of lipotropism hepatic factor; 14-30 parts of glyceryl monostearate; 3-7 parts of bile acid salt; 1-5 parts of L-ascorbyl palmitate. The special emulsifier for aquatic animals provided by the invention can improve the utilization rate of grease, reduce the occurrence of fatty liver of aquatic animals and improve the production level of aquatic animals; meanwhile, the emulsifier provided by the invention can protect liver and promote gallbladder, and promote digestion and absorption of fat; and the feed-bound water can be efficiently locked, the shelf life is prolonged, the water pollution is reduced, and the production cost is reduced.

Description

Emulsifier special for aquatic animals and preparation method and use method thereof
Technical Field
The invention belongs to the technical field of animal additives, and relates to an emulsifier special for aquatic animals, and a preparation method and a use method thereof.
Background
With the development of modern aquaculture industry, complete compound feed is increasingly becoming the main feed source of aquatic livestock. Under the modern intensive culture mode, the general application of high-grade aquatic feeds enables the production performance of aquatic animals to be greatly exerted and improved. In order to meet the special nutritional requirements of aquatic animals, manufacturers often need to add oil and fat, including fish oil, soybean oil, rapeseed oil, and the like, into the feed. On one hand, the grease is not completely consistent with the grease demand of aquatic animals, and the utilization of the grease by the aquatic animals is limited; on the other hand, a certain amount of starch and high-level grease are added into the feed, and because energy conversion is incomplete, a large amount of fat is deposited on the liver to form fatty liver, so that the physiological function of the liver is damaged; meanwhile, the liver overload of the aquatic animals carries out the synthesis of nutrient substances and the decomposition of vivotoxin, thereby seriously damaging the liver function of the aquatic animals, influencing the secretion of bile and further causing the unsatisfactory digestion and absorption of the aquatic animals on oil.
The special emulsifier for the aquatic feed can solve the problems of poor oil digestion utilization rate and fatty liver, and can effectively promote the absorption of oil and the energy conversion. However, the traditional emulsifier is mostly compounded by adopting a chemical synthesis emulsifier, and has potential threat to the health of consumers and the defects of low emulsifying capacity and the like.
CN102696895A discloses a biological emulsifier for aquatic animal feed, which is prepared from the following components: two or more of sophorolipid, rhamnolipid and algal glycolipid. Sophorolipid, rhamnolipid, algal glycolipid and the like in the emulsifier are secondary metabolites generated by microbial fermentation, can be naturally metabolized and degraded, cannot cause drug residues of aquatic products, and cannot influence water quality; but does not meet the fat demand of aquatic animals. CN105166577A discloses a compound emulsifier for improving the fat utilization rate of aquatic feeds, which comprises sucrose fatty acid ester, modified phospholipid, stearoyl calcium lactate, fatty acid monoglyceride, glycerin fatty acid ester, L-carnitine, bile acid, silybum marianum extract and lipase. The compound emulsifier provided in the patent application has a good emulsifying effect and can exist stably, but the compound emulsifier only aims at tilapia, weever and carassius auratus gibelio and is lack of wide applicability.
Therefore, it is necessary to provide a special emulsifier widely applicable to aquatic animals to meet the application requirements.
Disclosure of Invention
The invention aims to provide an emulsifier special for aquatic animals, and a preparation method and a use method thereof. The special emulsifier for aquatic animals provided by the invention can improve the utilization rate of grease, reduce the occurrence of fatty liver of aquatic animals and improve the production level of aquatic animals; meanwhile, the emulsifier provided by the invention can protect liver and promote gallbladder, and promote digestion and absorption of fat; and can reduce water pollution and production cost.
In order to achieve the purpose, the invention adopts the following technical scheme:
in a first aspect, the invention provides a special emulsifier for aquatic animals, which comprises the following components in parts by weight:
Figure RE-GDA0002499713690000021
the emulsifier provided by the invention can improve the digestion and absorption of grease, simultaneously supplements fatty liver resistant factors, can reduce the incidence rate of the fatty liver of aquatic animals to a greater extent, increases the organism health of the aquatic animals, and improves the production level. In addition, bile acid is supplemented in the form of bile acid salt, so that on one hand, the cracking of the oil can be promoted, and the aquatic livestock can digest and absorb the oil more quickly and sufficiently; on the other hand, the ionic bile acid salt is added, and as the bile acid participates in liver metabolism, the surface tension of fat can be reduced, so that the fat is emulsified into micro-droplets, and the action area of pancreatic lipase is increased; the bile acid can also combine with fatty acid to form water soluble complex, promote fatty acid absorption, reduce fatty deposition in liver, protect liver, promote gallbladder function, and promote fat digestion and absorption.
In the present invention, the sodium caseinate is 5 to 15 parts, such as 6 parts, 8 parts, 10 parts, 12 parts, 14 parts, etc.
In the present invention, the modified phospholipid is 24 to 40 parts, for example, 25 parts, 28 parts, 30 parts, 32 parts, 35 parts, 38 parts, etc.
In the present invention, the sucrose fatty acid ester is 15 to 30 parts, for example, 18 parts, 20 parts, 22 parts, 25 parts, 28 parts, etc.
In the present invention, the lipotropic factor is 4-10 parts, such as 5 parts, 6 parts, 7 parts, 8 parts, 9 parts and the like.
In the present invention, the glyceryl monostearate is 14-30 parts, such as 15 parts, 18 parts, 20 parts, 22 parts, 25 parts, 28 parts and the like.
In the present invention, the bile acid salt is 3 to 7 parts, for example, 4 parts, 5 parts, 6 parts, etc.
In the present invention, the L-ascorbyl palmitate is 1-5 parts, such as 2 parts, 3 parts, 4 parts, etc.
In the present invention, the modified phospholipid is an acylated modified phospholipid.
The modified phospholipid has acetone insoluble matter over 95%, HLB value of 8-13, acid value less than 30mgKOH/g, and viscosity (Cp,25 deg.c) less than 12000.
In the present invention, the lipotropic factor includes any one or a combination of at least two of choline, methionine, betaine, carnitine, lysine, or inositol.
Preferably, the fatty liver resistant factor comprises 20-40 parts of choline, 10-25 parts of inositol, 15-30 parts of methionine and 18-35 parts of carnitine by taking the total mass of the fatty liver resistant factor as 100 parts.
The substance is specifically selected as the anti-fatty liver factor, so that the invention has the advantage of having more remarkable influence on the fat metabolism of aquatic animals; meanwhile, the invention specifically optimizes the components contained in the lipotropic factor, and when the composition is within the limited range of the invention, the composition has the beneficial effects of facilitating the absorption and utilization of fat by aquatic animals, reducing the content of liver fat and improving the utilization rate of feed.
In the present invention, the choline is 20 to 40 parts, such as 25 parts, 30 parts, 35 parts, etc.
In the present invention, the inositol is 10-25 parts, such as 12 parts, 15 parts, 18 parts, 20 parts, 22 parts, etc.
In the present invention, the methionine is 15 to 30 parts, for example, 18 parts, 20 parts, 22 parts, 25 parts, 28 parts, etc.
In the present invention, carnitine is present in 18-35 parts, such as 20 parts, 22 parts, 25 parts, 28 parts, 30 parts, 32 parts, etc.
Preferably, the bile acid salt comprises any one of Taurocholate (TCA), Cholate (CA) and Deoxycholate (DCA) or a combination of at least two thereof.
Preferably, the bile acid salt is a combination of taurocholic acid, cholic acid and deoxycholic acid in a mass ratio of 1:1: 2.
The specific bile acid salt is specifically selected, so that the fat digestion and absorption promoting composition has the characteristic of relatively excellent fat digestion and absorption promoting effect, and if the ratio is not the same, the fat digestion and absorption rate is negatively influenced.
In a second aspect, the invention provides a preparation method of the aquatic animal special emulsifier according to the first aspect, and the preparation method comprises the steps of heating and mixing the components in the formula amount until the components are completely dissolved, and performing spray drying to obtain the emulsifier.
Preferably, the heating temperature is 50-70 ℃, such as 55 ℃, 60 ℃, 65 ℃ and the like.
In a third aspect, the invention provides a method for using the aquatic animal specific emulsifier according to the first aspect, wherein the method for using the aquatic animal specific emulsifier comprises the following steps:
adding the emulsifier into the feed and mixing uniformly.
Preferably, the use method comprises the following steps:
(1) heating and mixing the emulsifier and water to obtain a mixture;
(2) spraying part of the mixture into the feed, granulating, and spraying the rest of the mixture onto the surface of the granules;
preferably, the mixture also comprises grease.
When the emulsifier is uniformly mixed with oil and water and sprayed into the feed, the using effect is optimal.
Preferably, the heating temperature is 40-50 deg.C, such as 42 deg.C, 45 deg.C, 48 deg.C, etc.
Preferably, the emulsifier is added in an amount of 0.1-0.5%, such as 0.2%, 0.3%, 0.4%, etc., by mass of the feed.
Preferably, the volume of the portion of the mixture is 60-70% of the total volume of the mixture, such as 62%, 65%, 67%, etc.
Compared with the prior art, the invention has the following beneficial effects:
(1) the emulsifier provided by the invention can improve the digestion and absorption of grease, simultaneously supplements fatty liver resistant factors, can reduce the incidence rate of the fatty liver of aquatic animals to a greater extent, increases the organism health of the aquatic animals, and improves the production level;
(2) the emulsifier provided by the invention can solve the problems of poor oil digestion utilization rate and fatty liver, can effectively promote the absorption of oil and the conversion of energy, and simultaneously solves the problems of oil waste and water body pollution for aquatic feed, thereby realizing the aim of green culture; when the emulsifier provided by the invention is mixed into the feed of the gift tilapia, the weight gain rate of the gift tilapia is more than 845%, the specific growth rate is more than 3.5%, the feed coefficient is below 1.30, the survival rate is more than 80%, and the liver-body ratio is below 1.6%; in the preferable technical scheme of the invention, the weight gain rate of the tilapia is more than 885%, the specific growth rate is more than 4%, the feed coefficient is less than 1.05, the survival rate is 100%, and the liver-to-body ratio is less than 1.42%.
Detailed Description
The technical solution of the present invention is further explained by the following embodiments. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.
Example 1
The special emulsifier for the aquatic animals comprises the following components in parts by weight:
Figure RE-GDA0002499713690000061
wherein the modified phospholipid is purchased from four-dimensional co-creation trade company Limited in Shenzhen, and the sucrose fatty acid ester is purchased from Zhengzhou Yu and food additive company Limited; glycerol monostearate was purchased from Zhengzhou Kangyuan chemical products, Inc.
The fatty liver resistant factor comprises 32 parts of choline, 17 parts of inositol, 22 parts of methionine and 29 parts of carnitine by taking the mass of the fatty liver resistant factor as 100 parts.
The bile acid salt is a composition of sodium taurocholate, sodium cholate and sodium deoxycholate in a mass ratio of 1:1: 2.
The preparation method comprises the following steps:
mixing and heating the components according to the formula amount to 60 ℃, stirring until the components are completely dissolved, and obtaining the special emulsifier for aquatic animals.
Example 2
The special emulsifier for the aquatic animals comprises the following components in parts by weight:
Figure RE-GDA0002499713690000071
wherein, the modified phospholipid is purchased from four-dimensional co-creation trade Limited company in Shenzhen city;
the fatty liver resistant factor comprises 20 parts of choline, 15 parts of inositol, 30 parts of methionine and 35 parts of carnitine by taking the mass of the fatty liver resistant factor as 100 parts.
The bile acid salt is a composition of sodium taurocholate, sodium cholate and sodium deoxycholate in a mass ratio of 1:1: 2.
The preparation method comprises the following steps:
the components with the formula ratio are mixed and heated to 50 ℃, stirred to be completely dissolved, and the special emulsifier for aquatic animals is obtained.
Example 3
The special emulsifier for the aquatic animals comprises the following components in parts by weight:
Figure RE-GDA0002499713690000072
Figure RE-GDA0002499713690000081
wherein, the modified phospholipid is purchased from four-dimensional co-creation trade Limited company in Shenzhen city;
the fatty liver resistant factor is composed of 40 parts of choline, 25 parts of inositol, 17 parts of methionine and 18 parts of carnitine by taking the mass of the fatty liver resistant factor as 100 parts.
The bile acid salt is a composition of sodium taurocholate, sodium cholate and sodium deoxycholate in a mass ratio of 1:1: 2.
The preparation method comprises the following steps:
mixing and heating the components according to the formula amount to 70 ℃, stirring until the components are completely dissolved, and obtaining the special emulsifier for aquatic animals.
Example 4
The only difference from example 1 is that in this example, the bile acid salt is a combination of sodium taurocholate, sodium cholate and sodium deoxycholate in a mass ratio of 2:1: 1.
Example 5
The only difference from example 1 is that in this example, the bile acid salt is a combination of sodium taurocholate and sodium deoxycholate in a mass ratio of 1: 2.
Example 6
The only difference from example 1 is that in this example, the bile acid salt in example 1 is sodium cholate.
Example 7
The difference from example 1 is that in this example, the lipotropic factor is composed of 40 parts of choline, 30 parts of inositol, and 30 parts of methionine, based on 100 parts of the lipotropic factor by mass.
Example 8
The difference from example 1 is that, in this example, the lipotropic hepatic factor is lysine.
Comparative example 1
The difference from example 1 is that in this comparative example bile acid salt was replaced by cholic acid.
Comparative examples 2 to 3
The difference from example 1 is that in this comparative example, the modified phospholipid was 20 parts by weight (comparative example 2) and 45 parts by weight (comparative example 3).
Comparative examples 4 to 5
The difference from example 1 is that in the comparative example, the weight parts of the lipotropic factor are 1 part (comparative example 4) and 15 parts (comparative example 5).
Comparative examples 6 to 7
The difference from example 1 is that in this comparative example, the weight parts of bile acid salt are 0.5 parts (comparative example 6) and 12 parts (comparative example 7).
Comparative examples 8 to 9
The difference from example 1 is that in this comparative example, the sucrose fatty acid ester was 10 parts by weight (comparative example 8) and 35 parts by weight (comparative example 9).
Comparative examples 10 to 11
The difference from example 1 is that in this comparative example, the weight parts of glyceryl monostearate were 10 parts (comparative example 10) and 35 parts (comparative example 11).
Performance testing
The aquatic animal special emulsifier provided by the examples 1-8 and the comparative examples 1-11 is subjected to a performance test by the following method:
mixing an emulsifier sample with water at 45 ℃ to obtain a mixture, spraying 70% of the mixture into a basic feed, granulating, and spraying the rest mixture onto the surface of granules to obtain an improved feed;
wherein the addition amount of the emulsifier is 0.5% of the feed quality, and the basic feed is purchased from Guangdong Hengxing feed industry Co.
The animal experiment method is as follows:
experimental materials: the gift tilapia fries are purchased from a tilapia fine breeding field in Guangdong province, and after domestication for 2 weeks in an indoor circulating water aquaculture system of animal science research institute of Guangdong province, young fishes with regular specifications and health are selected for experiments. Each feed is provided with 2 repeated jars, 10 fish in each jar, and the initial average mass is 15.38 +/-0.05 g.
The breeding mode is as follows: the breeding test is 8 weeks, the feeding is carried out for 2 times (8:00 and 17:00) per day after the full feeding, an indoor daylight lamp is adopted as a light source, and the photoperiod is 12:12 in the day and at night; during the test period, the excrement at the bottom of the pot is sucked out by using a siphon pipe every morning, and then water is changed for each pot by about one third; fishing out the dead fish in time, and recording the weight after weighing; except the periods of bottom suction and water change and bait casting, the aeration and oxygenation are continuously carried out for the rest of time, the dissolved oxygen in the water is higher than 6.0 mg/L, the water temperature is maintained at 29.0 +/-2.0 ℃, the pH value is 7.0 +/-0.4, and the ammonia nitrogen concentration is lower than 0.2 mg/L.
The test method comprises the following steps:
after the experiment is finished, fasting the fish for 24 hours, randomly taking 3 fishes from each cylinder, placing the fishes into water containing 0.01% of 2-phenoxythanol anesthetic for anesthesia, then taking blood from the tail vein by using a 1mL syringe, and injecting the blood into a 1.5mL centrifuge tube; standing the blood at 4 deg.C for 2-5h, centrifuging at 4 deg.C and 3500r/min for 10min, transferring the supernatant into a new centrifuge tube, quickly freezing in liquid nitrogen, and storing in a refrigerator at-80 deg.C for subsequent analysis. Dissecting the fish after blood collection, putting liver, muscle and foregut samples into a centrifuge tube, quickly freezing in liquid nitrogen, storing in a refrigerator at-80 ℃ for later use, and finally taking 2 fish from each jar for measuring the texture characteristics of the muscle, and taking another 2 fish for measuring the nutritional ingredients of the whole fish; all the fishes are wiped dry by a wet towel, weighed one by one and the weight is recorded;
a) the weight gain rate is: according to "(W)f-Wi)/Wi× 100% 100 "calculation;
b) specific growth rate: according to "(lnW)f-lnWi) The/t × 100% "calculation;
c) the feed coefficient is as follows: according to "Da/(Wt-W0) "calculating;
d) survival rate: according to "Af/Ai× 100% 100 "calculation;
e) and (3) fullness: according to "Wb/Lb 3× 100% 100 "calculation;
f) liver to body ratio: according to "Wl/Wb× 100% 100 "calculation;
g) body ratio of zang organs: according to "Wv/Wb× 100% 100 "calculation;
wherein, WiAnd WfInitial and final average fish body mass, g, respectively; t is the final cultivation days, d; daG (dry matter) for total feed intake; wtAnd W0Initial and final total fish body weight, g, respectively; a. theiAnd AfInitial and final fish body numbers respectively; wbIs the fish body mass; l isbIs the length of the fish body, cm; wlAnd WvRepresenting liver and internal organ weight, g, respectively;
h) and (3) biochemical index determination: serum total cholesterol (T-CHO), Triglyceride (TG), high density lipoprotein (HDL-C) and low density lipoprotein (LDL-C) are all determined by adopting a kit of Nanjing institute of Biotechnology;
the test results are shown in tables 1 and 2:
TABLE 1
Figure RE-GDA0002499713690000111
Figure RE-GDA0002499713690000121
TABLE 2
Figure RE-GDA0002499713690000122
Figure RE-GDA0002499713690000131
According to the embodiment and the performance test, the emulsifier provided by the invention can improve the digestion and absorption of grease, reduce the incidence rate of fatty liver of aquatic animals and improve the body health of the aquatic animals; wherein the weight gain rate of the gift tilapia is more than 845 percent, the specific growth rate is more than 3.5 percent, the feed coefficient is less than 1.30 percent, the survival rate is more than 80 percent, and the liver-body ratio is less than 1.6 percent; in the preferable technical scheme of the invention, the weight gain rate of the tilapia is more than 885%, the specific growth rate is more than 4%, the feed coefficient is less than 1.05, the survival rate is 100%, and the liver-to-body ratio is less than 1.42%.
As can be seen from the comparison between example 1 and examples 4-6, the preferable bile acid salt in the invention is taurocholate, cholate and deoxycholate which are combined in a mass ratio of 1:1:2, and the finally obtained emulsifier has better action effect; as can be seen from the comparison between example 1 and examples 7-8, the fatty liver disease-resistant factor of the present invention has better effects when it comprises 20-40 parts of choline, 10-25 parts of inositol, 15-30 parts of methionine and 18-35 parts of carnitine.
As can be seen from the comparison between the example 1 and the comparative example 1, the ionic bile acid salt added in the invention can better play the roles of protecting liver and benefiting gallbladder and promoting fat digestion and absorption; as can be seen from the comparison of example 1 with comparative examples 2 to 11, the amounts of the components of the present invention added need to be within the limits of the present invention in order to have a better technical effect.
Performance test 2
The performance test of the special emulsifier for aquatic animals provided in example 1 is carried out by the following method:
mixing the emulsifier sample with the basic feed, and granulating to obtain an improved feed;
wherein the addition amount of the emulsifier is 0.5% of the feed quality, and the basic feed is purchased from Guangdong Hengxing feed industry Co.
Animal experimental methods were performed with reference to the method of performance test 1;
the test results are shown in tables 3 and 4:
TABLE 3
Figure RE-GDA0002499713690000141
TABLE 4
Sample (I) Serum total cholesterol Triglycerides High density lipoprotein Low density lipoprotein
Example 1 5.53 0.45 0.53 0.56
Examples 1 to 1 5.52 0.46 0.54 0.58
Example 1 shows the method of use of the emulsifier the method provided in performance test 1 and example 1-1 shows the method of use of the emulsifier as provided in performance test 2.
As can be seen from the comparison between example 1 and example 1-1, in the method of using the emulsifier, the emulsifier is mixed with water (or water and oil) and then mixed with the basic feed by spraying, so that the emulsifier has better effect.
The applicant states that the present invention is illustrated by the above examples to provide a special emulsifier for aquatic animals and a method for preparing and using the same, but the present invention is not limited to the above detailed method, i.e. it is not meant that the present invention must rely on the above detailed method to be implemented. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.

Claims (10)

1. The special emulsifier for aquatic animals is characterized by comprising the following components in parts by weight:
Figure FDA0002419821250000011
2. the aquatic animal specific emulsifier of claim 1, wherein the modified phospholipid is an acylated modified phospholipid.
3. The aquatic animal specific emulsifier of claim 1 or 2, wherein the lipotropic factor comprises any one or a combination of at least two of choline, methionine, betaine, carnitine, lysine or inositol.
4. The aquatic animal special emulsifier according to claim 3, wherein the lipotropic factor comprises 20-40 parts of choline, 10-25 parts of inositol, 15-30 parts of methionine and 18-35 parts of carnitine based on 100 parts of total mass of the lipotropic factor.
5. An aquatic animal specific emulsifier according to any one of claims 1-4, wherein the bile acid salt comprises any one or a combination of at least two of taurocholate, cholate and deoxycholate.
6. The aquatic animal special emulsifier according to claim 5, wherein the bile acid salt is a combination of taurocholate, cholate and deoxycholate in a mass ratio of 1:1: 2.
7. The method for preparing the aquatic animal special emulsifier according to any one of claims 1-6, wherein the method comprises heating and mixing the components in the formula amount until the components are completely dissolved, and spray drying to obtain the emulsifier.
8. The method of claim 7, wherein the heating temperature is 50 to 70 ℃.
9. Method of use of an aquatic animal specific emulsifier according to any one of claims 1-6, wherein the method of use comprises the steps of:
adding the emulsifier into the feed and uniformly mixing;
preferably, the use method comprises the following steps:
(1) heating and mixing the emulsifier and water to obtain a mixture;
(2) spraying part of the mixture into the feed, granulating, and spraying the rest of the mixture onto the surface of the granules;
preferably, the mixture also comprises grease.
10. The use according to claim 9, wherein the temperature of the heating is 40-50 ℃;
preferably, the addition amount of the emulsifier is 0.1-0.5% of the mass of the feed;
preferably, the volume of the portion of the mixture is 60 to 70% of the total volume of the mixture.
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