CN111329988A - Donkey-hide gelatin peptide buccal tablet rich in free arginine and preparation method thereof - Google Patents
Donkey-hide gelatin peptide buccal tablet rich in free arginine and preparation method thereof Download PDFInfo
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- CN111329988A CN111329988A CN201911265167.XA CN201911265167A CN111329988A CN 111329988 A CN111329988 A CN 111329988A CN 201911265167 A CN201911265167 A CN 201911265167A CN 111329988 A CN111329988 A CN 111329988A
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- extract
- donkey
- hide gelatin
- buccal tablet
- enzymolysis
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Abstract
The invention discloses a donkey-hide gelatin peptide buccal tablet rich in free arginine and a preparation method thereof, and is characterized in that the donkey-hide gelatin peptide buccal tablet consists of donkey-hide gelatin oligopeptide, a chrysanthemum extract, a tea extract, a lotus plumule extract, a dried orange peel extract and a momordica grosvenori extract, wherein the content of the donkey-hide gelatin oligopeptide is more than 80 percent, the content of the free arginine is more than 4 percent, and the free arginine can quickly enter arterial blood vessels of a human body through oral absorption and is absorbed and utilized by the human body. The donkey-hide gelatin peptide buccal tablet can be prepared by the following method: dissolving colla Corii Asini in water, adding chymotrypsin for enzymolysis for a certain time, adding papain for enzymolysis, inactivating enzyme, adding flos Chrysanthemi extract, folium Camelliae sinensis extract, plumula Nelumbinis extract, pericarpium Citri Tangerinae extract, and sweet stevia extract, spray granulating, and tabletting to obtain colla Corii Asini peptide buccal tablet. The preparation method is simple, the pH value can be adjusted by avoiding using chemical additives through a well-designed enzymolysis process, the arginine in the donkey-hide gelatin can be released to the maximum extent, the content of the free arginine in the obtained donkey-hide gelatin peptide buccal tablet is more than 4 percent, the donkey-hide gelatin peptide buccal tablet can be quickly absorbed and utilized through the oral cavity, the mouth feel is good, the internal heat is not caused, and the donkey-hide gelatin peptide buccal tablet is convenient to store, carry and use.
Description
Technical Field
The invention belongs to the field of polypeptide preparation in biotechnology, and particularly relates to a donkey-hide gelatin peptide buccal tablet rich in free arginine and a preparation method thereof.
Technical Field
Arginine is an important di-basic amino acid, known as an important carrier for transporting and storing amino acids in the body, and is extremely important in intramuscular metabolism. Arginine is less synthesized in the human body and needs to be partially supplemented from food, while it is an essential amino acid for the middle-aged and elderly people.
Nitric oxide NO is widely distributed in various tissues in the living body, particularly in nerve tissues. It was evaluated as a star molecule in 1992 by the American Science journal. NO is an extremely unstable biological free radical, whose production depends on Nitric Oxide Synthase (NOs) and has important biological effects in cardiovascular and cerebrovascular regulation, nervous regulation, immune regulation, and the like. Therefore, the method is generally regarded by people.
Nitric oxide in the human body has two sources: one is non-enzymatic, from the body surface or chemical degradation and conversion of ingested inorganic nitrogen; the first is enzymatic, produced by the enzyme nitric oxide synthase catalyzing the removal of guanidino from L-arginine. The enzymatic nitric oxide is derived from arginine, a precursor of nitric oxide. The arginine-rich food taken into human body can produce nitric oxide in vivo through enzyme and exert physiological functions.
Because arginine is a precursor of nitric oxide, supplementing arginine can obviously improve the content of nitric oxide in vivo, and nitric oxide can contact and relax muscle cells in arteries, so that arterial blood vessels are expanded, blood pressure is reduced, blood flow is improved, and the risk of cardiovascular and cerebrovascular diseases can be effectively reduced. On the contrary, when the arginine content in the body is deficient, the nitric oxide content in the body is reduced, namely, the possibility of sudden cardiovascular and cerebrovascular diseases is greatly increased.
Once nitric oxide is produced in the body, it contacts and relaxes muscle cells in the arteries, dilating the arteries, which reduces blood pressure and improves blood flow. In addition, nitric oxide prevents blood from clotting at dangerous sites. If blood clots in the heart or brain, the patient may suffer a heart attack or stroke. As long as the human body produces a sufficient amount of nitric oxide, the risk of cardiovascular and cerebrovascular diseases is greatly reduced, and therefore, the nitric oxide can effectively reduce blood pressure and prevent stroke and heart disease.
Arginine is a natural product, an amino acid from proteins, and is found in many sources, including chicken, fish, and certain vegetables. However, arginine in ordinary food is often low in content, and the amount of generated nitric oxide is small, so that the food does not have related nutritional value.
Relevant studies at home and abroad prove that the food rich in arginine can reduce the harm of hypertension and cardiovascular and cerebrovascular diseases, and the physiological function of the food is related to that arginine can effectively promote the generation of nitric oxide in vivo. High fat diets can lead to a decrease in endothelial dependent vasodilatory function in the body, resulting in increased blood pressure, decreased blood flow, and increased blood viscosity. Arginine can effectively promote the generation of nitric oxide in vivo and can reduce the degradation of nitric oxide by in vivo oxidized lipid, thereby improving the content of nitric oxide in vivo.
The colla Corii Asini is a block prepared by removing hair from skin of donkey of Equidae, and has effects of nourishing yin, tonifying blood, and preventing miscarriage. It is used to treat cough due to blood deficiency, cough due to asthenia, hematemesis, epistaxis, hematochezia, menoxenia, metrorrhagia, and fetal leakage. Modern medical research shows that the content of arginine in donkey-hide gelatin is very high, and can reach 6% -8%, but the arginine exists in a polypeptide form and cannot be directly absorbed and utilized. The protein in the donkey-hide gelatin is mainly collagen, the digestion and absorption of which need the participation of related protease, and the adaptation population is often suffered from weakness of the spleen and the stomach or gastrointestinal diseases, and taking the donkey-hide gelatin not only causes the burden of the digestive system of a patient, but also can not effectively exert the drug effect, and is easy to cause 'excessive internal heat'.
With the development of modern biological enzymolysis technology, in recent years, there are increasing reports on the research of preparing donkey-hide gelatin into donkey-hide gelatin peptide by using biological enzymolysis technology. CN102021215A reports a method for extracting colla Corii Asini peptide, which utilizes alkaline protease, trypsin and flavourzyme, and focuses on the molecular weight of colla Corii Asini peptide. CN101294186A utilizes pepsin and trypsin to carry out enzymolysis on colla Corii Asini, and focuses on the size and distribution of colla Corii Asini peptide molecular weight.
The existing preparation technology of the donkey-hide gelatin peptide mostly concentrates on the size and the distribution of the molecular weight of the donkey-hide gelatin peptide, arginine in the donkey-hide gelatin peptide cannot be well released, the arginine exists in a polypeptide form, the content of free arginine is lower than 1 percent, and the arginine can be absorbed and utilized after being digested, so that the utilization rate of the arginine is influenced.
Disclosure of Invention
Aiming at various problems existing in the utilization process of the existing donkey-hide gelatin peptide, the invention provides a simple and convenient preparation method of a donkey-hide gelatin peptide buccal tablet. The method is simple to operate, no chemical addition is needed in the whole process, the content of free arginine in the donkey-hide gelatin peptide buccal tablet is more than 4%, the donkey-hide gelatin peptide buccal tablet can be quickly absorbed and utilized by a human body in a buccal mode, and the defects of low arginine absorption utilization rate, low speed and the like are overcome on the premise of keeping the active function of polypeptide. The technical scheme of the invention is realized in the following mode.
(1) Dissolving: adding 3-10 times of deionized water into colla Corii Asini block, heating to 85 deg.C, and dissolving completely;
(2) enzymolysis: adding papain into the donkey-hide gelatin solution obtained in the step (1), performing enzymolysis for 2 hours in 3 days, then adding trypsin, and continuing enzymolysis for 2 hours;
(3) enzyme deactivation: heating the enzymolysis liquid obtained in the step (3) to inactivate enzyme and then cooling to below 30 ℃;
(4) blending: adding 0.01-0.03 time of chrysanthemum extract, 0.01-0.05 time of tea extract, 0.01-0.03 time of lotus plumule extract, 0.01-0.03 time of dried orange peel extract and 0.01-0.03 time of fructus momordicae extract into the liquid obtained in the step (3);
(5) and (3) granulation: spray drying and granulating the liquid obtained in the step (4), wherein the air inlet temperature is 150 ℃ and 180 ℃, and the air outlet temperature is 70-90 ℃;
(6) tabletting: and (5) directly tabletting the granules obtained in the step (5) to obtain the donkey-hide gelatin peptide buccal tablet.
Compared with the prior art, the technical scheme of the invention is simpler to operate, the molecular weight of the enzymolysis solution can be accurately controlled without a membrane separation step, in addition, through elaborate design, the papain and the chymotrypsin which comprise arginine in enzyme cutting sites are used, the chymotrypsin is firstly used for enzymolysis for 2 hours, the pH of the system just reaches the optimal enzymolysis condition of the papain, and the papain is then added for enzymolysis for 2 hours, so that the optimal enzymolysis condition can be reached by avoiding adding a chemical additive to adjust the pH value, the operation is simple, and the method is very suitable for large-scale industrial production; the content of free arginine in the donkey-hide gelatin peptide product produced by the scheme is more than 4 percent, and arginine can be absorbed by the oral cavity by adopting a buccal tablet form, quickly reach human artery and be absorbed and utilized by the human body; and by adding chrysanthemum extract, tea extract, lotus plumule extract, dried orange peel extract and momordica grosvenori extract which have the function of clearing heat and removing toxicity into the donkey-hide gelatin peptide, the excessive internal heat symptom caused by eating the donkey-hide gelatin can be avoided.
DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
The present invention is described in detail by the following specific examples, which should be construed as merely illustrative of the preparation of a polypeptide with a controllable molecular weight range, and not as a limitation of the present invention, and can be modified or improved without departing from the spirit of the present invention.
Example 1
Taking 250 g of donkey-hide gelatin blocks, adding the donkey-hide gelatin blocks into 750 g of deionized water, heating to 85 ℃, stirring to completely dissolve the donkey-hide gelatin blocks, and cooling to 40 ℃; then adding 50U of chymotrypsin for enzymolysis for 2 hours, then adding 50U of papain for continuous enzymolysis for 2 hours; then heating to 100 ℃ for 20 minutes, and cooling to 30 ℃; then adding 0.5 g of chrysanthemum extract, 0.4 g of tea extract, 0.2 g of lotus plumule extract, 0.5 g of dried orange peel extract and 0.1 g of clerodendrum chinense extract, stirring uniformly, and directly spray-drying at the air inlet temperature of 170 ℃ and the air outlet temperature of 80 ℃ to obtain the donkey-hide gelatin peptide particles. Then pressing into buccal tablet by tablet machine under 50kN pressure.
The liquid chromatography analysis result shows that the content of free amino acid in the prepared donkey-hide gelatin peptide is 4.3%.
Example 2
Taking 250 g of donkey-hide gelatin blocks, adding the donkey-hide gelatin blocks into 1000 g of deionized water, heating to 85 ℃, stirring to completely dissolve the donkey-hide gelatin blocks, and cooling to 45 ℃; adding 40U of chymotrypsin for enzymolysis for 2 hours, then adding 40U of papain for continuous enzymolysis for 2 hours; then heating to 100 ℃ for 20 minutes, and cooling to 30 ℃; then adding 0.4 g of chrysanthemum extract, 0.6 g of tea extract, 0.3 g of lotus plumule extract, 0.6 g of dried orange peel extract and 0.1 g of clerodendrum chinense extract, stirring uniformly, and directly spray-drying at the air inlet temperature of 170 ℃ and the air outlet temperature of 80 ℃ to obtain the donkey-hide gelatin peptide particles. Then pressing into buccal tablet by tablet machine under 50kN pressure.
The liquid chromatography analysis result shows that the content of free amino acid in the prepared donkey-hide gelatin peptide is 4.5%.
Comparative example 1
Taking 250 g of donkey-hide gelatin blocks, adding the donkey-hide gelatin blocks into 1000 g of deionized water, heating to 85 ℃, stirring to completely dissolve the donkey-hide gelatin blocks, and cooling to 45 ℃; then adding 100U of papain, and carrying out enzymolysis for 4 hours; then heating to 100 ℃ for 20 minutes, and cooling to 30 ℃; and (3) directly performing spray drying after uniformly stirring, wherein the air inlet temperature is 170 ℃, and the air outlet temperature is 80 ℃ to obtain the donkey-hide gelatin peptide particles.
The liquid chromatography analysis result shows that the content of free amino acid in the prepared donkey-hide gelatin peptide is 0.8 percent.
Comparative example 2
Taking 250 g of donkey-hide gelatin blocks, adding the donkey-hide gelatin blocks into 1000 g of deionized water, heating to 85 ℃, stirring to completely dissolve the donkey-hide gelatin blocks, and cooling to 45 ℃; then 100U of trypsin is added for enzymolysis for 4 hours; then heating to 100 ℃ for 20 minutes, and cooling to 30 ℃; and (3) directly performing spray drying after uniformly stirring, wherein the air inlet temperature is 170 ℃, and the air outlet temperature is 80 ℃ to obtain the donkey-hide gelatin peptide particles.
The liquid chromatography analysis result shows that the content of free amino acid in the prepared donkey-hide gelatin peptide is 0.9 percent.
Comparative example 3
Taking 250 g of donkey-hide gelatin blocks, adding the donkey-hide gelatin blocks into 1000 g of deionized water, heating to 85 ℃, stirring to completely dissolve the donkey-hide gelatin blocks, and cooling to 45 ℃; then 100U of pepsin is added for enzymolysis for 4 hours; then heating to 100 ℃ for 20 minutes, and cooling to 30 ℃; and (3) directly performing spray drying after uniformly stirring, wherein the air inlet temperature is 170 ℃, and the air outlet temperature is 80 ℃ to obtain the donkey-hide gelatin peptide particles.
The liquid chromatography analysis result shows that the content of free amino acid in the prepared donkey-hide gelatin peptide is 0.4%.
Claims (6)
1. The donkey-hide gelatin peptide buccal tablet rich in free arginine is characterized by comprising donkey-hide gelatin oligopeptide, a chrysanthemum extract, a tea extract, a lotus plumule extract, a dried orange peel extract and a momordica grosvenori extract, wherein the content of the donkey-hide gelatin oligopeptide is more than 80%, the content of the free arginine is more than 4%, and the donkey-hide gelatin peptide buccal tablet can rapidly enter arterial blood vessels of a human body through oral absorption, so that the effects of expanding blood vessels and improving blood flow are achieved.
2. The donkey-hide gelatin peptide buccal tablet rich in free arginine and the preparation method thereof as claimed in claim 1, characterized in that the preparation method comprises the following steps:
(1) dissolving: adding 3-10 times of deionized water into colla Corii Asini block, heating to 85 deg.C, and dissolving completely;
(2) enzymolysis: adding chymotrypsin into the donkey-hide gelatin solution obtained in the step (1) for enzymolysis for 2 hours, then adding papain, and continuing enzymolysis for 2 hours;
(3) enzyme deactivation: heating the enzymolysis liquid obtained in the step (3) to inactivate enzyme and then cooling to below 30 ℃;
(4) blending: adding 0.01-0.03 time of chrysanthemum extract, 0.01-0.05 time of tea extract, 0.01-0.03 time of lotus plumule extract, 0.01-0.03 time of dried orange peel extract and 0.01-0.03 time of fructus momordicae extract into the liquid obtained in the step (3);
(5) and (3) granulation: spray drying and granulating the liquid obtained in the step (4), wherein the air inlet temperature is 150 ℃ and 180 ℃, and the air outlet temperature is 70-90 ℃;
(6) tabletting: and (5) directly tabletting the granules obtained in the step (5) to obtain the donkey-hide gelatin peptide buccal tablet.
3. The preparation method according to claim 2, characterized in that in the enzymolysis in the step (2), firstly, 20-100U/L of chymotrypsin is added, the enzymolysis is carried out for 2 hours at 30-50 ℃, then, 20-100U/L of papain is added, and the enzymolysis is continued for 2 hours at 30-50 ℃; .
4. The colla Corii Asini peptide buccal tablet according to claims 1-3, characterized in that the content of free arginine in the obtained colla Corii Asini peptide buccal tablet is more than 4%.
5. The colla Corii Asini peptide buccal tablet according to claims 1-4, characterized in that the colla Corii Asini peptide buccal tablet is composed of colla Corii Asini oligopeptide, flos Chrysanthemi extract, folium Camelliae sinensis extract, plumula Nelumbinis extract, pericarpium Citri Tangerinae extract, and sweet stevia extract.
6. The colla corii asini peptide buccal tablet according to claims 1 to 5, which is applied to the fields of blood enriching, blood activating, auxiliary blood pressure lowering foods, medicines and the like.
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