CN111321104A - Preparation method of compound organic trace element feed additive for laying hens - Google Patents
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Abstract
The invention provides a preparation method of a compound organic trace element feed additive for laying hens, which is characterized in that enterococcus faecalis subjected to tolerance domestication is selected to ferment a culture medium containing iron, copper, zinc and manganese with certain concentrations, so that the iron, copper, zinc and manganese are enriched and further converted into organic iron, copper, zinc and manganese, and the organic iron, copper, zinc and manganese are obtained through centrifugation. Then, the microbial thallus is mixed with a diluent and dried, so as to prepare the compound organic trace element feed additive, and the method can lead the organic iron, copper, zinc and manganese to account for 37.8%, 72.3%, 81.8% and 57.3%. The composite organic trace element prepared by the method has definite element enrichment amount and organic rate indexes, can be added into daily ration of laying hens according to an accurate proportion, does not need to be additionally supplemented with any four elements, and does not have the problem of excessive element.
Description
Technical Field
The invention belongs to the field of feed additives, and particularly relates to a preparation method of a compound organic trace element feed additive for laying hens.
Background
The trace elements of iron, copper, zinc and manganese are necessary for a series of life processes of animal growth, development, reproduction and the like. Deficiency or insufficiency of trace elements can cause deficiency and reduced productivity, even death of animals. Therefore, the supplement of a certain amount of trace elements in daily ration is a main way to meet the physiological needs of animals. Currently, trace element supplementation is mainly by inorganic forms, such as sulfate, chloride, oxide, and the like. The inorganic trace elements are active in nature and are easily converted into forms which are difficult to utilize in feed processing, transportation and storage and in the digestive tract of animals, so that the absorption rate of the trace elements is low. Therefore, inorganic trace elements are often used in production by way of overdosing (exceeding the required amount specified by the standards of each country). However, this method results in increased loss of trace elements to the intestinal mucosa and increased excretion contamination of the animal. Researches suggest that some organic ligands can protect trace element ions from being interfered by anti-nutritional factors such as phytic acid in a digestive tract, so that organic trace elements such as organic acid state, EDTA complex state and amino acid chelate state are researched and developed, but due to the influence of technical methods, production processes and the like, the use effect of some commercially available organic trace elements is not ideal. In addition, most of the commercially available organic trace elements are single organic trace elements chelated by different amino acids, and other trace elements still need to be compounded in the animal production process. In addition, the required organic ligand is amino acid, and unreasonable compounding or long-term addition of certain amino acid easily causes the balance unbalance of the amino acid, thereby influencing the absorption and utilization of the whole amino acid of the feed.
The microorganisms are micro organisms with rapid propagation and strong adaptability in nature, wherein certain microorganisms can enrich trace elements and convert the trace elements into organic forms, so that organic trace elements with relatively balanced organic nutrients can be prepared. For example, the patent application No. 200910096744.7 discloses a method for producing a copper-rich yeast product containing 1052 mg copper per kg yeast by using yeast, screening copper-resistant strains, performing chemical mutagenesis to obtain a high copper-resistant strain, and fermenting the high copper-resistant strain in a culture solution with a copper concentration of 10 mug/mL. Patent application No. 201510027241.X discloses a copper-tolerant strain (bacillus licheniformis) obtained by screening known microorganisms with a copper-rich medium, and fermenting a solid medium containing 0.85% copper with the strain to produce a fermented copper feed additive. The patent application No. 201510515658.0 discloses that a copper feed additive in a yeast fermentation state with a copper content of 7.58g/kg can be produced by fermenting a combination of brewer's yeast and Candida utilis in a liquid medium with a copper concentration of 1200 mg/L. In addition, the fermented manganese, iron, zinc and selenium feed additives are produced by microorganisms respectively under the conditions of patent application numbers 201510027281.4, 201510027313.0, 201510027253.2 and 201510027242.4.
However, it is worth noting that all the trace elements prepared by the invention patents are single trace element products, and other kinds of trace elements need to be compounded in the practical application process. Secondly, the products prepared by the invention are fermentation mixture products, and the problems of whether the trace elements are converted into organic states, the organic rate and the like are not clear. Finally, the mixed fermentation product of the existing feed additive is large in addition amount generally, the product quality is not well controlled, and the mixed fermentation product is not beneficial to industrial production and product standard formulation.
Disclosure of Invention
In order to solve the problems, the invention provides a preparation method of a compound organic trace element feed additive for laying hens, which can simultaneously enrich iron, copper, zinc and manganese and convert the iron, copper, zinc and manganese into organic iron, copper, zinc and manganese so as to produce the compound organic trace element feed additive which can be directly added into daily ration of the laying hens.
In order to achieve the purpose, the invention adopts the technical scheme that:
a preparation method of a compound organic trace element feed additive for laying hens is characterized in that microorganisms are used for enriching inorganic trace elements, and the preparation method comprises the following steps:
screening and domesticating enterococcus faecalis, wherein the enterococcus faecalis is subjected to concentration gradient domestication of single elements of iron, copper, zinc and manganese and concentration gradient domestication of mixed elements of iron, copper, zinc and manganese respectively to obtain trace element-resistant enterococcus faecalis;
step two, inoculating the trace element-resistant enterococcus faecalis obtained in the step one into a fermentation culture medium containing iron, copper, zinc and manganese, wherein the addition proportion of the seed liquid is 15% -30%, and the elements in the fermentation culture medium comprise: the concentration of iron element is 600mg/L, the concentration of copper element is 150mg/L, the concentration of zinc element is 500mg/L and the concentration of manganese element is 350 mg/L;
monitoring the pH value of the inoculated fermentation medium in real time, adjusting the pH value of the fermentation medium to 6.8-7.2, performing segmented speed-controlled fermentation for 72 hours, and adding nutrients, mineral salts and a pH adjusting reagent into the inoculated fermentation medium in a flowing manner in the fermentation process;
and step four, centrifuging after fermentation to obtain wet thalli, adding a diluent into the wet thalli, uniformly mixing, and drying the uniformly mixed wet thalli to obtain the compound organic trace element feed additive for the laying hens.
Further, in the first step, enterococcus faecalis is acclimatized on single element culture media of an iron-containing gradient culture medium, a copper-containing gradient culture medium, a zinc-containing gradient culture medium and a manganese-containing gradient culture medium respectively, and then screening and acclimatizing culture are performed in a mixed element gradient culture medium with four-factor four-level, and the highest respective tolerance element concentration of the trace element-resistant enterococcus faecalis obtained by acclimatization under a single element is as follows: 2000mg/L of iron, 800mg/L of copper, 2500mg/L of zinc and 2500mg/L of manganese, and the highest tolerable element concentration under the mixed elements is 1200mg/L of iron, 550mg/L of copper, 1800mg/L of zinc and 1500mg/L of manganese.
Further, in the mixed element gradient medium with four factors and four levels, the gradient concentration of iron comprises 800mg/L, 1000mg/L, 1200mg/L and 1500mg/L, the gradient concentration of copper comprises 350mg/L, 450mg/L, 550mg/L and 650mg/L, the gradient concentration of zinc comprises 1500mg/L, 1800mg/L, 2100mg/L and 2500mg/L, and the gradient concentration of manganese comprises 1200mg/L, 1500mg/L, 1800mg/L and 2200 mg/L.
Further, in the second step, every 1000 parts of the fermentation medium containing iron, copper, zinc and manganese comprises the following components: 6 parts of glucose, 60 parts of wheat middling, 2.5 parts of urea, 16.5 parts of soybean meal, 0.5 part of monopotassium phosphate, 1.5 parts of sodium chloride, 2.8 parts of ferrous sulfate, 0.6 part of copper sulfate, 2.2 parts of zinc sulfate, 1.1 parts of manganese sulfate and 906.3 parts of concentrated liquor of industrial yellow water of alcohol collected by a 10-time diluted plate frame.
Further, in the third step, the nutrients comprise a carbon source and a nitrogen source, the carbon source comprises glucose and magnetic powder, and the nitrogen source comprises urea and soybean meal powder; the mineral salts include potassium dihydrogen phosphate, sodium chloride, ferrous sulfate, copper sulfate, zinc sulfate and manganese sulfate.
Further, in the third step, in the fermentation process, a carbon source and a nitrogen source are fed every 2 hours, and the pH is adjusted by a pH adjusting reagent; adding potassium dihydrogen phosphate, sodium chloride, ferrous sulfate, copper sulfate, zinc sulfate and manganese sulfate every 6 h.
Further, the pH value of the fermentation medium is adjusted by ammonia water.
Further, in the third step, the segmented speed-control fermentation also comprises four stages: the first stage is as follows: when the fermentation time is 0-12h, the stirring speed is 550-720 r/min; and a second stage: when the fermentation time is 13-24h, the stirring speed is 400-450 r/min; and a third stage: when the fermentation time is 25-48h, the stirring speed is 300-350 r/min; a fourth stage: the fermentation time is 49-72h, and the stirring speed is 200-250 r/min.
Further, the pH value of the fermentation medium is monitored in real time through a pH monitoring probe, a data line and a control instrument.
Further, in the fourth step, the wet thalli obtained after centrifugation are added with light calcium carbonate and mixed evenly, and the weight ratio of the light calcium carbonate to the wet thalli is 1: 1.
The invention has the beneficial effects that:
1. according to the preparation method of the composite organic trace element feed additive for the laying hens, the domesticated enterococcus faecalis has tolerance to four elements of iron, copper, zinc and manganese at the same time, can enrich the four elements at the same time, and is converted into organic iron, copper, zinc and manganese;
2. on the basis of the highest tolerance concentration of mixed trace elements, the enrichment amount of a certain element is determined by fixing the concentration and the enrichment amount of the element, and the concentrations of other trace elements are respectively and correspondingly reduced, so that the finally determined optimal trace element concentrations are respectively 600mg/L of iron, 150mg/L of copper, 500mg/L of zinc and 350mg/L of manganese, the element proportion is reasonable, and the enrichment amount proportion of the four elements of iron, copper, zinc and manganese meets the physiological requirements of laying hens;
3. the composite organic trace element provided by the invention has definite element enrichment amount and organic rate indexes, can be added into daily ration of laying hens according to an accurate proportion, does not need to be additionally supplemented with four elements, and does not have the problem of excessive element. By the method, microbial biomass produced by each liter of culture solution can reach 113.3 g, and after dilution, each kilogram of the additive contains 63000-65000 mg of iron, 4000-4200 mg of copper, 41000-42000 mg of zinc and 26000-28000 mg of manganese, wherein the organic iron, copper, zinc and manganese account for 37.8%, 72.3%, 81.8% and 57.3%. The compound organic microelement is mainly used for daily ration of laying hens. Animal experiments show that compared with inorganic trace elements (specified according to NRC (1994) standard), the product can meet the requirements of laying hens for iron, copper, zinc and manganese when the specified amount of the standard is 60% and 80%, the laying rate is respectively improved by 2.86% and 4.02%, and the egg breaking rate is respectively reduced by 27.15% and 18.33%.
Detailed Description
In order that those skilled in the art will be able to better understand the technical solutions provided by the present invention, the following description is provided in connection with specific embodiments.
The biomass, enrichment amount and organic ratio of the invention are respectively as follows:
(1) biomass, after fermentation, drying to about 10% of water content to obtain the weight of the thallus, namely the biomass;
(2) the trace element enrichment amount refers to the amount of iron, copper, zinc and manganese contained in a unit microorganism and is expressed by mg/kg dry bacteria. The determination method comprises the steps of digesting microorganisms by adopting wet digestion, determining the contents of iron, copper, zinc and manganese in the microorganisms by using an inductively coupled plasma spectrometer, and calculating the total contents of iron, copper, zinc and manganese in the microorganisms;
(3) and (3) the trace element organization rate is that the microorganism is broken by adopting ultrasonic waves, the centrifugation is carried out at 5000r/min, the supernatant is obtained, and the free trace elements in the supernatant are measured by an inductively coupled plasma spectrometer. The content of organic elements is obtained by subtracting the amount of free elements from the total amount of corresponding trace elements in the microorganism, and the organic ratio index of various trace elements is obtained by dividing the total amount by the content.
Example 1
1. Seed culture
Screening and domesticating enterococcus faecalis, wherein the enterococcus faecalis is respectively screened and domesticated in an iron-containing gradient culture medium, a copper-containing gradient culture medium, a zinc-containing gradient culture medium, a manganese-containing gradient culture medium and a mixed element gradient culture medium containing iron (800, 1000, 1200, 1500 mg/L), copper (350, 450, 550, 650 mg/L), zinc (1500, 1800, 2100, 2500 mg/L) and manganese (1200, 1500, 1800, 2200 mg/L), and the domesticated trace element-resistant enterococcus faecalis has the highest respective tolerable element concentration under single element: 2000mg/L of iron, 800mg/L of copper, 2500mg/L of zinc and 2500mg/L of manganese, and the highest tolerable element concentration under the mixed elements is 1200mg/L of iron, 550mg/L of copper, 1800mg/L of zinc and 1500mg/L of manganese.
Inoculating the obtained microelement-resistant enterococcus faecalis to a liquid culture medium, wherein the liquid culture medium comprises 0.6 part of glucose, 5.0 parts of soluble starch, 0.2 part of peptone, 0.6 part of soybean meal, 0.11 part of potassium dihydrogen phosphate, 0.15 part of sodium chloride and 93.34 parts of deionized water, adjusting the pH value to 6.8, and culturing for 18h to obtain a seed solution, wherein the concentration of enterococcus faecalis not lower than 0.75 × 10 per milliliter of the seed solution8。
2. Preparation of copper fermentation Medium
Taking 18 parts of glucose, 180 parts of wheat middling, 7.5 parts of urea, 49.5 parts of soybean meal, 1.5 parts of monopotassium phosphate, 4.5 parts of sodium chloride, 8.4 parts of ferrous sulfate, 1.8 parts of copper sulfate, 6.6 parts of zinc sulfate and 3.3 parts of manganese sulfate, firstly mixing with 1000mL of concentrated liquor of the industrial yellow water collected by a plate frame (diluted by 10 times before), then quantifying to 3000mL with the concentrated liquor of the industrial yellow water (diluted by 10 times before), and adjusting the pH to 6.8.
3. Inoculating seeds
Mixing the trace element-resistant enterococcus faecalis seed liquid into the fermentation culture medium according to the volume ratio of 15%, and uniformly mixing.
4. Fermentation culture
Loading the inoculated fermentation medium into a 10L fermentation tank for segmented speed-controlled fermentation, and setting the fermentation parameter as the temperature of 32 ℃; the stirring frequency is 0-12 h: 550r/min, 13-24 h: 450r/min, 25-48 h: 350r/min, 49-72 h: 200 r/min. Monitoring the pH value of fermentation liquor by a pH value monitoring system (mainly comprising a pH monitoring probe, a data line and a control instrument), detecting the consumption of a carbon source, a nitrogen source and mineral salt, establishing a change parameter between the pH change and the consumption of nutrients and mineral salt, and respectively feeding the carbon source, the nitrogen source and the mineral substance when the pH value is reduced by 0.5%; ammonia was added to the reaction solution every time the pH was lowered by "1", and the pH was adjusted to 6.8.
5. Preparation and index detection of composite organic trace element feed additive
And after the fermentation is finished, centrifuging to obtain wet microbial cells (wet cells), uniformly mixing the wet cells with light calcium carbonate according to the proportion of 1:1 (w/w), and drying at the constant temperature of 75 ℃ until the water content is about 10 percent to obtain the composite organic trace element feed additive.
Taking 0.5g of additive, digesting by a wet digestion method to obtain digestion solution, measuring the contents of iron, copper, zinc and manganese by an inductively coupled plasma spectrometer, and calculating the enrichment amount of various trace elements. Taking 0.5g of additive, adding 5mL of deionized water, uniformly mixing, crushing cells by using an ultrasonic crusher, centrifuging at 5000r/min to obtain supernatant, measuring various free state trace elements by using an inductively coupled plasma spectrometer, subtracting the free state elements from the total amount of the measured trace elements to obtain organic trace elements, and dividing by the total amount of the trace elements to obtain the organic rate index of the trace elements, namely the organic rate of iron, copper, zinc and manganese is not lower than 37%, 72%, 81% and 57%.
Example 2
1. Seed culture
Screening and acclimatization culture of microelement-resistant enterococcus faecalis similar to example 1, and the microelement-resistant enterococcus faecalis is inoculated into liquid culture mediumWherein the liquid medium comprises 0.6 part of glucose, 5.0 parts of soluble starch, 0.2 part of peptone, 0.6 part of soybean meal, 0.11 part of potassium dihydrogen phosphate, 0.15 part of sodium chloride and 93.34 parts of deionized water, adjusting the pH value to 7.0, and culturing for 18h to obtain a seed solution, wherein the content of enterococcus faecalis in each milliliter of the seed solution is not less than 0.75 × 108。
2. Preparation of copper fermentation Medium
Taking 18 parts of glucose, 180 parts of wheat middling, 7.5 parts of urea, 49.5 parts of soybean meal, 1.5 parts of monopotassium phosphate, 4.5 parts of sodium chloride, 8.4 parts of ferrous sulfate, 1.8 parts of copper sulfate, 6.6 parts of zinc sulfate and 3.3 parts of manganese sulfate, firstly mixing with 1000mL of concentrated liquor of the industrial yellow water collected by a plate frame (diluted by 10 times before), then quantifying to 3000mL with the concentrated liquor of the industrial yellow water (diluted by 10 times before), and adjusting the pH to 7.0.
3. Inoculating seeds
Mixing the microelement-resistant enterococcus faecalis seed liquid into the fermentation culture medium according to the volume ratio of 20%, and mixing uniformly.
4. Fermentation culture
Loading the inoculated fermentation medium into a 10L fermentation tank, and setting the fermentation parameter to be 35 ℃; the stirring frequency is 0-12 h: 720r/min, 13-24 h: 400r/min, 25-48 h: 300r/min, 49-72 h: 250 r/min; respectively feeding a carbon source, a nitrogen source and mineral substances when the pH is reduced by 0.5; ammonia was added to the reaction solution every time the pH was lowered by "1", and the pH was adjusted to 7.0.
5. Preparation and index detection of composite organic trace element feed additive
And after the fermentation is finished, centrifuging to obtain wet microbial cells (wet cells), uniformly mixing the wet cells with light calcium carbonate according to the proportion of 1:1 (w/w), and drying at the constant temperature of 75 ℃ until the water content is about 10 percent to obtain the composite organic trace element feed additive.
Taking 0.5g of additive, digesting by a wet digestion method to obtain digestion solution, measuring the contents of iron, copper, zinc and manganese by an inductively coupled plasma spectrometer, and calculating the enrichment amount of various trace elements. Taking 0.5g of additive, adding 5mL of deionized water, uniformly mixing, crushing cells by using an ultrasonic crusher, centrifuging at 5000r/min to obtain supernatant, measuring various free state trace elements by using an inductively coupled plasma spectrometer, subtracting the free state elements from the total amount of the measured trace elements to obtain organic trace elements, and dividing by the total amount of the trace elements to obtain the organic rate index of the trace elements, namely the organic rate of iron, copper, zinc and manganese is not lower than 37%, 72%, 81% and 57%. .
Example 3
1. Seed culture
Screening and domestication culture of microelement-resistant enterococcus faecalis similar to example 1, inoculating microelement-resistant enterococcus faecalis into a liquid culture medium, wherein the liquid culture medium comprises glucose 0.6 parts, soluble starch 5.0 parts, peptone 0.2 parts, soybean meal 0.6 parts, potassium dihydrogen phosphate 0.11 parts, sodium chloride 0.15 parts, and deionized water 93.34 parts, adjusting pH to 7.2, and culturing for 18h to obtain a seed solution, wherein each milliliter of the seed solution contains enterococcus faecalis not less than 0.75 × 10 parts8。
2. Preparation of copper fermentation Medium
Taking 18 parts of glucose, 180 parts of wheat middling, 7.5 parts of urea, 49.5 parts of soybean meal, 1.5 parts of monopotassium phosphate, 4.5 parts of sodium chloride, 8.4 parts of ferrous sulfate, 1.8 parts of copper sulfate, 6.6 parts of zinc sulfate and 3.3 parts of manganese sulfate, firstly mixing with 1000mL of concentrated liquor of the industrial yellow water collected by a plate frame (diluted by 10 times before), then quantifying to 3000mL with the concentrated liquor of the industrial yellow water (diluted by 10 times before), and adjusting the pH to 7.2.
3. Inoculating seeds
Mixing the trace element-resistant enterococcus faecalis seed liquid with the fermentation culture medium according to the volume ratio of 30%, and mixing uniformly.
4. Fermentation culture
Loading the inoculated fermentation medium into a 10L fermentation tank, and setting the fermentation parameter to be 33 ℃; the stirring frequency is 0-12 h: 600r/min, 13-24 h: 500r/min, 25-48 h: 350r/min, 49-72 h: 250 r/min; respectively feeding a carbon source, a nitrogen source and mineral substances when the pH is reduced by 0.5; ammonia was added to the reaction solution every time the pH was lowered by "1", and the pH was adjusted to 7.2.
5. Preparation and index detection of composite organic trace element feed additive
And after the fermentation is finished, centrifuging to obtain wet microbial cells, uniformly mixing the wet microbial cells with light calcium carbonate according to the proportion of 1:1 (w/w), and drying at the constant temperature of 75 ℃ until the water content is about 10 percent to obtain the composite organic trace element feed additive.
Taking 0.5g of additive, digesting by a wet digestion method to obtain digestion solution, measuring the contents of iron, copper, zinc and manganese by an inductively coupled plasma spectrometer, and calculating the enrichment amount of various trace elements. Taking 0.5g of additive, adding 5mL of deionized water, uniformly mixing, crushing cells by using an ultrasonic crusher, centrifuging at 5000r/min to obtain supernatant, measuring various free state trace elements by using an inductively coupled plasma spectrometer, subtracting the free state elements from the total amount of the measured trace elements to obtain organic trace elements, and dividing by the total amount of the trace elements to obtain the organic rate index of the trace elements, namely the organic rate of iron, copper, zinc and manganese is not lower than 37%, 72%, 81% and 57%.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. The present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. A preparation method of a compound organic trace element feed additive for laying hens is characterized in that microorganisms are used for enriching inorganic trace elements, and the preparation method comprises the following steps:
screening and domesticating enterococcus faecalis, wherein the enterococcus faecalis is subjected to concentration gradient domestication of single elements of iron, copper, zinc and manganese and concentration gradient domestication of mixed elements of iron, copper, zinc and manganese respectively to obtain trace element-resistant enterococcus faecalis;
step two, inoculating the trace element-resistant enterococcus faecalis obtained in the step one into a fermentation culture medium containing iron, copper, zinc and manganese, wherein the addition proportion of the seed liquid is 15% -30%, and the elements in the fermentation culture medium comprise: the concentration of iron element is 600mg/L, the concentration of copper element is 150mg/L, the concentration of zinc element is 500mg/L and the concentration of manganese element is 350 mg/L;
monitoring the pH value of the inoculated fermentation medium in real time, adjusting the pH value of the fermentation medium to 6.8-7.2, performing segmented speed-controlled fermentation for 72 hours, and adding nutrients, mineral salts and a pH adjusting reagent into the inoculated fermentation medium in a flowing manner in the fermentation process;
and step four, centrifuging after fermentation to obtain wet thalli, adding a diluent into the wet thalli, uniformly mixing, and drying the uniformly mixed wet thalli to obtain the compound organic trace element feed additive for the laying hens.
2. The preparation method of the compound organic trace element feed additive for laying hens according to claim 1, which is characterized by comprising the following steps: in the first step, enterococcus faecalis is domesticated on single element culture mediums of an iron-containing gradient culture medium, a copper-containing gradient culture medium, a zinc-containing gradient culture medium and a manganese-containing gradient culture medium respectively, then screening and domesticating culture are carried out in a mixed element gradient culture medium with four factors and four levels, and the highest respective tolerance element concentration of the domesticated trace element enterococcus faecalis is: 2000mg/L of iron, 800mg/L of copper, 2500mg/L of zinc and 2500mg/L of manganese, and the highest tolerable element concentration under the mixed elements is 1200mg/L of iron, 550mg/L of copper, 1800mg/L of zinc and 1500mg/L of manganese.
3. The preparation method of the compound organic trace element feed additive for laying hens according to claim 2, which is characterized by comprising the following steps: four-factor four-level mixed element gradient culture medium, gradient concentration of iron comprises 800mg/L, 1000mg/L, 1200mg/L and 1500mg/L, gradient concentration of copper comprises 350mg/L, 450mg/L, 550mg/L and 650mg/L, gradient concentration of zinc comprises 1500mg/L, 1800mg/L, 2100mg/L and 2500mg/L, and gradient concentration of manganese comprises 1200mg/L, 1500mg/L, 1800mg/L and 2200 mg/L.
4. The preparation method of the compound organic trace element feed additive for laying hens according to claim 1, which is characterized by comprising the following steps: in the second step, every 1000 parts of the fermentation medium of iron, copper, zinc and manganese comprises the following components: 6 parts of glucose, 60 parts of wheat middling, 2.5 parts of urea, 16.5 parts of soybean meal, 0.5 part of monopotassium phosphate, 1.5 parts of sodium chloride, 2.8 parts of ferrous sulfate, 0.6 part of copper sulfate, 2.2 parts of zinc sulfate, 1.1 parts of manganese sulfate and 906.3 parts of concentrated liquor of industrial yellow water of alcohol collected by a 10-time diluted plate frame.
5. The preparation method of the compound organic trace element feed additive for laying hens according to claim 1, which is characterized by comprising the following steps: in the third step, the nutrients comprise a carbon source and a nitrogen source, the carbon source comprises glucose and magnetic powder, and the nitrogen source comprises urea and soybean meal powder; the mineral salts include potassium dihydrogen phosphate, sodium chloride, ferrous sulfate, copper sulfate, zinc sulfate and manganese sulfate.
6. The preparation method of the compound organic trace element feed additive for laying hens according to claim 5, which is characterized by comprising the following steps: in the third step, in the fermentation process, adding a carbon source and a nitrogen source every 2h, and adjusting the pH value by a pH adjusting reagent; adding potassium dihydrogen phosphate, sodium chloride, ferrous sulfate, copper sulfate, zinc sulfate and manganese sulfate every 6 h.
7. The method for preparing the composite organic trace element feed additive for laying hens according to claim 1 or 6, which is characterized in that: the pH of the fermentation medium is also adjusted by means of ammonia.
8. The preparation method of the compound organic trace element feed additive for laying hens according to claim 1, which is characterized by comprising the following steps: in the third step, the segmented speed-controlled fermentation also comprises four stages: the first stage is as follows: when the fermentation time is 0-12h, the stirring speed is 550-720 r/min; and a second stage: when the fermentation time is 13-24h, the stirring speed is 400-450 r/min; and a third stage: when the fermentation time is 25-48h, the stirring speed is 300-350 r/min; a fourth stage: the fermentation time is 49-72h, and the stirring speed is 200-250 r/min.
9. The preparation method of the compound organic trace element feed additive for laying hens according to claim 1, which is characterized by comprising the following steps: the pH value of the fermentation medium is monitored in real time through a pH monitoring probe, a data line and a control instrument.
10. The preparation method of the compound organic trace element feed additive for laying hens according to claim 1, which is characterized by comprising the following steps: and in the fourth step, adding light calcium carbonate into the wet thalli obtained after centrifugation, and uniformly mixing, wherein the weight ratio of the light calcium carbonate to the wet thalli is 1: 1.
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