CN111297949A

CN111297949A - Essential oil mask and preparation method thereof

Info

Publication number: CN111297949A
Application number: CN202010196577.XA
Authority: CN
Inventors: 王鹏
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-03-19
Filing date: 2020-03-19
Publication date: 2020-06-19

Abstract

The invention belongs to the technical field of biomedicine, and particularly relates to an essential oil mask and a preparation method thereof. Essential oil gauze mask, including the gauze mask body, still including fixing at this internal pearl that explodes of gauze mask, explode the pearl and be used for breaking after the extrusion, explode the pearl and include the shell, the parcel has the essential oil in the shell, essential oil includes the following weight share's composition: 160 portions of tea tree oil, 140 portions of cnidium fruit oil, 120 portions of magnolia oil, 80 to 120 portions of patchouli oil, 140 portions of blumea oil, 180 portions of elsholtzia oil, 80 to 120 portions of rosemary oil, 160 portions of bay oil, 80 to 120 portions of geranium oil, 150 portions of peppermint oil and 150 portions of laggera oil. The essential oil mask has the function of inhibiting viruses by burying essential oil blasting beads. The technical scheme can be used for preventing respiratory diseases when respiratory infectious diseases are epidemic.

Description

Essential oil mask and preparation method thereof

Technical Field

The invention belongs to the technical field of biomedicine, and particularly relates to an essential oil mask and a preparation method thereof.

Background

The mask is a sanitary article worn at the mouth and nose part for filtering air entering the mouth and nose, can prevent harmful gas, smell and spray from entering and exiting the mouth and nose of a wearer, and is made of gauze, paper or non-woven fabrics and the like. The mask has a certain filtering function on air entering the lung, and has a very good protection function when the mask is worn for operation in an environment polluted by dust and the like when respiratory infectious diseases are prevalent. However, the mask in the prior art can only play a role of physically blocking pathogenic substances, and cannot actively kill pathogenic substances such as bacteria, viruses and the like. In the 21 st century, human beings suffer from various large-scale virus attacks, and influenza virus, SARS virus, MERS virus, novel coronavirus and the like continue to cause great harm to human society. The research and development of the mask with the effect of inhibiting or killing pathogenic substances are urgently needed, active defense and passive defense are managed together, and the protective effect of the mask on a wearer is improved.

Disclosure of Invention

The invention aims to provide an essential oil mask, which has the function of inhibiting viruses by burying essential oil blasting beads in a filling manner.

In order to solve the technical problems, the technical scheme of the invention is as follows:

the utility model provides an essential oil gauze mask, includes the gauze mask body, still including setting up at this internal pearl that explodes of gauze mask, it includes the shell that can break to explode the pearl, the parcel has essential oil in the shell, essential oil includes the composition of following parts by weight: 160 portions of tea tree oil, 140 portions of cnidium fruit oil, 120 portions of magnolia oil, 80 to 120 portions of patchouli oil, 140 portions of blumea oil, 180 portions of elsholtzia oil, 80 to 120 portions of rosemary oil, 160 portions of bay oil, 80 to 120 portions of geranium oil, 150 portions of peppermint oil and 150 portions of laggera oil.

By adopting the technical scheme, the technical principle is as follows: because the popping beads are fixed in the mask, when the mask is worn, the popping beads of the essential oil can be pinched off, the essential oil infiltrates the mask, the essential oil can play a role in inhibiting or killing pathogenic substances, and the mask can also play a role in physically blocking the external pathogenic substances. The essential oil content of the popsicle is as follows: tea tree oil is volatile oil (vegetable essential oil) extracted from Melaleuca alternifolia (Melaleuca alternifolia); cnidium fruit oil is volatile oil extracted from fruit of cnidium fruit (cnidiummonieri); magnolia bark oil is volatile oil extracted from root of Magnolia officinalis (Magnolia officinalis); patchouli oil is volatile oil extracted from whole plant of patchouli (Adenosmabuchneidenes); the oleum folium Artemisiae Argyi is volatile oil extracted from whole plant of folium Artemisiae Argyi (Artemisia argyi); elsholtzia oil is volatile oil extracted from the whole herb of Elsholtziaciliata; rosemary oil is volatile oil extracted from the whole herb of Rosmarinus officinalis (Rosmarinus officinalis), and laurel oil is volatile oil extracted from leaf of Laurus nobilis; pelargonium graveolens oil is volatile oil extracted from branches and leaves of Pelargonium graveolens (Pelargonium graveolens); peppermint oil is volatile oil extracted from the whole herb of peppermint (Mentha X piperita); laggera oil is volatile oil extracted from aerial parts of Laggera pterodonta. The inhibition effect on pathogenic substances is realized by the synergistic effect of various plant essential oils.

Wherein, the patchouli is the traditional spice plant for preventing and eliminating evil diseases, diminishing inflammation, sterilizing, repelling mosquitoes and preventing and treating cold in 400 years of the Hani nationality. Researchers have also performed both in vivo and clinical experiments in mice to verify the effect of patchouli oil in inhibiting influenza virus ("patchouli cold ointment experimental studies and field observations, chinese herbal medicine, 1987"), but the inhibitory effect of patchouli influenza virus needs to be achieved at higher doses. The inventor conducts a great deal of research on how to increase the virus inhibiting effect of the patchouli oil, and finds that the bay oil has synergistic effect on the anti-influenza virus effect of the patchouli oil although the bay oil has weak antiviral ability per se. The inventor further researches the action mechanism of the patchouli oil for inhibiting the influenza virus, and finds that the patchouli oil can inhibit the influenza virus in vitro, thereby inhibiting influenza virus infected cells, and further relieving the infection of the influenza virus to organisms, and the bay oil can enhance the in-vitro inhibition effect of the patchouli oil on the influenza virus. Due to the above mechanism, the patchouli oil is especially suitable for being applied to a mask, pathogenic substances such as viruses and the like can be blocked on the mask, and essential oil attached to the mask can simultaneously realize in-vitro inhibition on the pathogenic substances and reduce the infectivity and pathogenicity of the pathogenic substances.

Has the advantages that:

(1) when the multifunctional mask is used, the explosion beads are pinched and broken, the essential oil can infiltrate the mask, the essential oil attached to the mask volatilizes and can also play a role in inhibiting or killing pathogenic substances when the pathogenic substances are physically blocked by the mask, and the protection effect of the mask is improved. And when not wearing the gauze mask, explode the pearl and seal up the deposit with essential oil in inside, avoid essential oil to reveal and volatilize, guaranteed that essential oil can not volatilize or oxidation became invalid, and be convenient for transportation and storage.

(2) The essential oil in the populus is formed by compounding various plant extracts (mainly plant volatile oil), and integrates the effects of various volatile oils. The essential oil is used for removing pestilence, diminishing inflammation and sterilizing, removing evil and expelling dirt, refreshing and restoring consciousness, repelling mosquitoes and the like, and becomes an important traditional custom of people of all families in the world; in traditional Chinese medicine and pharmacology, the essential oil is considered to have the functions of clearing and activating the channels and collaterals, inducing resuscitation with aromatics and the like, has special fragrance and pharmacological activity, is a good material for aromatherapy, and meanwhile, on the mental level, the fragrance of the essential oil enables people to be delightful and has a certain conditioning effect.

(3) The laurel oil can promote inhibitory effect of oleum Rosae Laevigatae on influenza virus, and the combination of the two can effectively inhibit pathogenic substances and protect user health. The oleum Rosae Laevigatae can inhibit influenza virus in vitro, and the laurel oil can enhance the in vitro inhibitory effect of oleum Rosae Laevigatae on influenza virus.

(4) The essential oil mask is filled with the explosion beads containing essential oil, when the essential oil mask is used, the explosion beads containing the essential oil are pinched in the mask, and natural essential oil micromolecule compounds have the characteristics of strong volatility, activity and associativity, can be directly acted on an oral-nasal respiratory tract system, and avoid a short plate for orally taking medicines (the orally taken medicines can play the drug effect only through absorption, conversion, metabolism and other pharmacokinetic processes and through a long and complex action way). The essential oil mask has the characteristics of short action way and direct and quick generation of beneficial effects. In the way of virus infection, the essential oil mask can quickly play the roles of inhibiting virus and protecting human bodies (the essential oil micromolecule compound with extremely strong activity is easy to be compatible with virus macromolecules, so that the virus property is changed). The essential oil mask can be used for preventing human body from being infected with respiratory infectious diseases when the respiratory infectious diseases are epidemic.

Further, the magnolia oil is prepared by a carbon dioxide supercritical extraction method.

By adopting the technical scheme, the magnolia oil with high purity and good biological effect can be obtained.

Further, tea tree oil, cnidium fruit oil, patchouli oil, mugwort leaf oil, elsholtzia oil, rosemary oil, bay oil, geranium oil, peppermint oil and laggera oil are all prepared by steam distillation.

By adopting the technical scheme, the steam distillation method is a traditional essential oil extraction method, the operation is simple, and complex and expensive equipment is not needed.

Further, the patchouli oil is extracted from patchouli of Thymus of Scrophulariaceae.

By adopting the technical scheme, the essential oil extracted from the ophicalcitum (Adenosmabuchneidenes) (also called flea fleabane) of the plant of the genus Thymus of the family Scrophulariaceae mainly comprises terpenoids and phenolic compounds as effective chemical components. The oleum Rosae Laevigatae has antiviral effect and can kill pathogenic substances in upper respiratory tract. Clinical experiments show that the patchouli oil and the preparation thereof can prevent acute respiratory tract infection such as cold. If the patchouli oil is applied to the nasal and oral parts, the nasal obstruction can be relieved, and the ventilation is good; can prevent symptoms from aggravating after the application in the initial stage of the cold; it can also be applied to temple for treating dizziness and headache, and also has mosquito and insect bite preventing effect. Toxicological experiments show that the externally applied patchouli oil has no obvious side effect and skin allergy phenomenon.

Further, the bay oil is prepared by the following method: crushing fresh bay leaves to obtain bay leaf powder, carrying out cold leaching on the bay leaf powder by using ethyl acetate, and then filtering to obtain a solid phase to obtain a material A; performing enzymolysis treatment on the material A by using cellulase to obtain a material B; and (3) extracting the essential oil in the material B by using a steam distillation method to obtain the bay oil.

By adopting the technical scheme, the obtained bay oil has stronger promotion effect on the effect of the patchouli oil on inhibiting the influenza virus. The bay oil has a complex composition and is reported to contain 45 compounds including alkenes, alcohols, and the like. According to detection, the bay oil obtained after the ethyl acetate cold-soaking treatment has changed in component composition. The content of sesquiterpene oxide in the bay oil obtained by the technical scheme is reduced, the inventor analyzes that the sesquiterpene oxide has small synergistic effect on the patchouli oil, the substances are removed or partially removed due to the pretreatment of ethyl acetate, and the content of the component which has the effect of promoting the antiviral effect of the patchouli oil in the obtained bay oil is relatively increased. Therefore, the bay oil obtained by the technical scheme has stronger synergistic effect on the patchouli oil.

Furthermore, the dosage ratio of the laurel leaf powder to the ethyl acetate is 1g (5-9) ml, and the time for cold soaking the laurel leaf powder with the ethyl acetate is 1-2 h.

By adopting the technical scheme, the ethyl acetate treatment time is too short, and non-target functional components (components which cannot form a synergistic enhancement effect on the patchouli) cannot be effectively removed; and the long treatment time can cause the dissolution of the target functional components (the components which can form a synergistic effect on the patchouli oil). The time of the cold dipping treatment is a relatively critical factor.

Further, the material B is obtained by the following method: adding water and cellulase into the material A to obtain a system A; obtaining a system B after the system A is subjected to enzymolysis reaction; the material B is a solid phase in the system B.

By adopting the technical scheme, the cellulase breaks the cell wall structure, so that the intracellular components can be fully dissolved out, and the extraction efficiency is increased.

Further, the content of the cellulase in the system A is 1-2 wt%, the time of the enzymolysis reaction is 2-3h, the temperature of the enzymolysis reaction is 35-40 ℃, and the pH value of the enzymolysis reaction is 7.0-8.0.

By adopting the technical scheme, the enzymolysis reaction can be fully carried out.

Furthermore, the particle size of the blasting beads is 4.5-5.0 mm.

By adopting the technical scheme, the size is suitable for being loaded into a mask, and enough essential oil in the blasting beads is also ensured.

Further, the preparation method of the essential oil mask comprises the following steps:

(1) mixing tea tree oil, cnidium fruit oil, Magnolia officinalis oil, Pogostemon oil, folium Artemisiae Argyi oil, herba Moslae oil, Rosemary oil, laurel oil, Pelargonium graveolens oil, oleum Menthae Dementholatum and Lardizabalanopsis oil to obtain mixed oil;

(2) adding caprylic capric glyceride into the mixed oil, and diluting to obtain essential oil; or adding solubilizer and water into the mixed oil, and diluting to obtain the essential oil.

By adopting the technical scheme, the oil-soluble essential oil can be prepared by adding the caprylic capric glyceride into the mixed oil, and the water-soluble essential oil can be prepared by adding the solubilizer and the water into the mixed oil. Wherein, the water-soluble essential oil has higher bioavailability and better antiviral effect.

Drawings

Fig. 1 is a diagram of an example of the present invention 1.

Detailed Description

Example 1:

the utility model provides an essential oil gauze mask, includes the gauze mask body, and the gauze mask body is the gauze mask commonly used among the prior art, and the gauze mask body includes the oronasal occlusion part and is located the hangers portion of oronasal occlusion part both sides, and oronasal occlusion part stacks by double-deck thick gauze and forms, and double-deck thick gauze edge is sewed up. Each layer of thick gauze is formed by stacking a plurality of single-layer thin gauzes, the single-layer thin gauzes are sewn and fixed, and blasting beads are bonded between two adjacent layers of thick gauzes (the blasting beads are clamped between the double-layer thick gauzes). The number of the blasting beads can be determined according to actual needs, the blasting beads are spherical, and the particle size is 4.5-5.0 mm. The popball is formed by wrapping essential oil by a shell, the shell material (which is high molecular polymer and mainly comprises caprylic capric glyceride) of the popball is provided by Balanophora japonica biotechnology limited, and the popball is also produced by processing generation of the company (the inventor provides essential oil, and the company wraps the essential oil in the shell material), and the physical diagram of the popball is shown in figure 1. When the mask is used, essential oil in the popping beads can be released only by pinching and popping the popping beads, and the double-layer thick gauze is soaked. When a person wears the mask, the mask not only has the functions of filtering air and blocking external pathogenic substances, but also has the function of inhibiting viruses due to the essential oil on the mask.

The essential oil in the blasting beads comprises the following components in parts by weight: 140 parts of tea tree oil, 120 parts of cnidium fruit oil, 140 parts of mangnolia officinalis oil, 100 parts of patchouli oil, 120 parts of blumea oil, 140 parts of elsholtzia oil, 100 parts of rosemary oil, 130 parts of bay oil, 100 parts of geranium oil, 120 parts of peppermint oil and 130 parts of laggera oil. Tea tree oil, cnidium fruit oil, patchouli oil, blumea oil, elsholtzia oil, rosemary oil, bay oil, geranium oil, peppermint oil and laggera oil are all prepared by steam distillation, and magnolia oil is prepared by carbon dioxide supercritical extraction. The steam distillation method and the carbon dioxide supercritical extraction method are the existing mature plant essential oil extraction processes, and except for the patchouli oil and the laurel oil, other plant essential oils are purchased. The plant raw material is extracted by steam distillation or supercritical carbon dioxide extraction to obtain volatile oil, which is a conventional method in the prior art and will not be described repeatedly.

The preparation method of the patchouli oil comprises the following steps: pulverizing 50g fresh herba Cisii Japonici (whole plant), and sieving with 40 mesh sieve to obtain herba Cisii Japonici powder. Putting the patchouli powder into a round-bottomed flask, adding 300ml of cold water, installing a distillation device, heating and distilling (electrically heating, setting the temperature to be 110 ℃) until the first drop of liquid flows out of a condensation pipe, using a conical flask to hold and receive distillate for 3 hours, collecting the distillate after the distillation is finished, using a separating funnel to separate oil phase and water phase in the distillate, and using anhydrous sodium sulfate to treat the oil phase for 24 hours to obtain the patchouli oil. The preparation method of the bay oil comprises the following steps: pulverizing 50g fresh laurel leaf, and sieving with 40 mesh sieve to obtain laurel leaf powder. Adding 350ml ethyl acetate into laurel leaf powder, cold soaking for 2 hr, and filtering to obtain solid phase to obtain material A (laurel leaf powder soaked with ethyl acetate). And dispersing the material A in 50ml of pure water, and adding cellulase to obtain a system A, wherein the content of the cellulase in the system A is 2 wt%. Then, the enzymolysis reaction is carried out for 2.5h at the temperature of 37 ℃ and the pH value of 7.0. After the reaction is finished, obtaining a system B (containing the laurel leaf powder after enzymolysis, namely the material B), adding 300ml of pure water to obtain a distillation system, and carrying out steam distillation extraction on the distillation system. And (3) installing a distillation device, heating and distilling (electrically heating, setting the temperature to be 110 ℃) until the first drop of liquid flows out of the condensation pipe, using a conical flask to contain distillate, wherein the distillation time is 2 hours, collecting the distillate after the distillation is finished, using a separating funnel to separate an oil phase and a water phase in the distillate, and using anhydrous sodium sulfate to treat the oil phase for 24 hours to obtain the bay oil.

Mixing tea tree oil, cnidium fruit oil, Magnolia officinalis oil, oleum Rosae Laevigatae, folium Artemisiae Argyi oil, herba Moslae oil, Rosemary oil, laurel oil, Pelargonium graveolens oil, oleum Menthae Dementholatum and Lardizabalanus oil to obtain mixed oil, and adding water and solubilizer SF410 (Simplicol, Simplicin). The addition amount of the solubilizer SF410 is 2-4 times of the mixed oil, in the embodiment, the addition amount of the solubilizer SF410 is 4 times of the mixed oil, and the balance is pure water. The mixed oil is diluted to one tenth of the original mixed oil (in practice, the mixed oil can be diluted to one fifteenth to one eighth of the original mixed oil), and the essential oil (namely the water-soluble essential oil which contains 1 part of the mixed oil, 4 parts of the solubilizer SF410 and 5 parts of pure water) is obtained and is used for preparing the water-soluble blasting beads.

Example 2

This example is substantially the same as example 1, except that the preparation method of bay oil: pulverizing 50g fresh laurel leaf, and sieving with 40 mesh sieve to obtain laurel leaf powder. Adding 250ml ethyl acetate into laurel leaf powder, cold soaking for 2 hr, and filtering to obtain solid phase to obtain material A (laurel leaf powder soaked with ethyl acetate). And dispersing the material A in 50ml of pure water, and adding cellulase to obtain a system A, wherein the content of the cellulase in the system A is 1 wt%. Then carrying out enzymolysis reaction for 3h at 40 ℃ and pH value of 8.0. After the reaction is finished, obtaining a system B (containing the laurel leaf powder after enzymolysis), adding 300ml of pure water to obtain a distillation system, and carrying out steam distillation extraction on the distillation system. And (3) installing a distillation device, heating and distilling (electrically heating, setting the temperature to be 110 ℃) until the first drop of liquid flows out of the condensation pipe, using a conical flask to contain distillate, wherein the distillation time is 2 hours, collecting the distillate after the distillation is finished, using a separating funnel to separate an oil phase and a water phase in the distillate, and using anhydrous sodium sulfate to treat the oil phase for 24 hours to obtain the bay oil.

The difference is also that the formula of the essential oil is as follows: 160 parts of tea tree oil, 140 parts of cnidium fruit oil, 160 parts of mangnolia officinalis oil, 120 parts of verbena oil, 140 parts of blumea oil, 180 parts of elsholtzia oil, 120 parts of rosemary oil, 160 parts of bay oil, 120 parts of geranium oil, 150 parts of peppermint oil and 150 parts of laggera oil.

This example prepared an oily essential oil, which was a mixture of tea tree oil, cnidium seed oil, magnolia oil, patchouli oil, mugwort leaf oil, elsholtzia oil, rosemary oil, bay oil, geranium oil, peppermint oil, and pteridan oil. And adding food-grade caprylic capric glyceride (MCT) into the mixed oil, diluting the mixed oil to one tenth of the original mixed oil (in actual operation, the mixed oil can be diluted to one fifteenth to one eighth of the original mixed oil), and obtaining essential oil (oil-soluble essential oil, wherein the essential oil contains 1 part of mixed oil and 9 parts of MCT), and the essential oil is used for preparing oily popcorns.

Example 3

This example is substantially the same as example 1, except that the preparation method of bay oil: pulverizing 50g fresh laurel leaf, and sieving with 40 mesh sieve to obtain laurel leaf powder. Adding 450ml ethyl acetate into laurel leaf powder, cold soaking for 1h, and filtering to obtain solid phase to obtain material A (laurel leaf powder soaked with ethyl acetate). And dispersing the material A in 50ml of pure water, and adding cellulase to obtain a system A, wherein the content of the cellulase in the system A is 2 wt%. Then carrying out enzymolysis reaction for 2h at 35 ℃ and pH value of 7.0. After the reaction is finished, obtaining a system B (containing the laurel leaf powder after enzymolysis), adding 300ml of pure water to obtain a distillation system, and carrying out steam distillation extraction on the distillation system. And (3) installing a distillation device, heating and distilling (electrically heating, setting the temperature to be 110 ℃) until the first drop of liquid flows out of the condensation pipe, using a conical flask to contain distillate, wherein the distillation time is 2 hours, collecting the distillate after the distillation is finished, using a separating funnel to separate an oil phase and a water phase in the distillate, and using anhydrous sodium sulfate to treat the oil phase for 24 hours to obtain the bay oil.

The difference is also that the formula of the essential oil is as follows: 120 parts of tea tree oil, 100 parts of cnidium fruit oil, 120 parts of mangnolia officinalis oil, 80 parts of verbena oil, 100 parts of blumea oil, 120 parts of elsholtzia oil, 80 parts of rosemary oil, 100 parts of bay oil, 80 parts of geranium oil, 100 parts of peppermint oil and 100 parts of laggera oil.

Comparative example 1

This comparative example is substantially the same as example 1, except that the preparation process of bay oil (direct distillation, no distillation pretreatment step): pulverizing 50g fresh laurel leaf, and sieving with 40 mesh sieve to obtain laurel leaf powder. Laurel leaf powder was charged into a round-bottomed flask, and 300ml of pure water was added to obtain a distillation system, which was subjected to steam distillation extraction. And (3) installing a distillation device, heating and distilling (electrically heating, setting the temperature to be 110 ℃) until the first drop of liquid flows out of the condensation pipe, using a conical flask to contain distillate, wherein the distillation time is 2 hours, collecting the distillate after the distillation is finished, using a separating funnel to separate an oil phase and a water phase in the distillate, and using anhydrous sodium sulfate to treat the oil phase for 24 hours to obtain the bay oil.

Comparative example 2

This comparative example is substantially the same as example 1, except that the bay oil was prepared by the method comprising: pulverizing 50g fresh laurel leaf, and sieving with 40 mesh sieve to obtain laurel leaf powder. Adding 350ml ethyl acetate into laurel leaf powder, cold soaking for 2 hr, and filtering to obtain solid phase to obtain material A (laurel leaf powder soaked with ethyl acetate). Directly adding 300ml of pure water into the material A without an enzymolysis step, and obtaining the bay oil after steam distillation.

Comparative example 3

This comparative example is substantially the same as example 1, except that the bay oil was prepared by the method comprising: pulverizing 50g fresh laurel leaf, and sieving with 40 mesh sieve to obtain laurel leaf powder. Adding 50ml of pure water into bay leaf powder, and adding cellulase to obtain a system A, wherein the content of the cellulase in the system A is 2 wt%. Then, the enzymolysis reaction is carried out for 2.5h at the temperature of 37 ℃ and the pH value of 7.0. After the reaction is finished, obtaining a system B (containing the laurel leaf powder after enzymolysis). Further adding 300ml of pure water to obtain a distillation system, and performing steam distillation extraction on the distillation system to obtain the bay oil.

Comparative example 4

This comparative example is essentially the same as example 1 except that bay oil is not added to the essential oil.

Comparative example 5

This comparative example is substantially the same as example 1 except that the lauryl leaf powder was cold-dipped with ethyl acetate for 5 hours.

Experimental example: viral inhibitory Effect

This example investigated the inhibitory effect of the test agent on the ability of the virus to infect after it has acted extracellularly with the virus. Experimental grouping was as follows:

(1) negative control group: the test reagent is sterilized double distilled water;

(2) positive control group: the test reagent is ribavirin injection (Chinese medicine standard H41023268), and the ribavirin injection is diluted by one tenth of sterilized double distilled water in a sterile environment to obtain the test reagent;

(3) experimental group 1: the test reagent is the essential oil (water-soluble essential oil) of example 1, and the essential oil needs to be subjected to filtration sterilization treatment and then to subsequent cell experiments;

(4) experimental group 2: the test reagent is the essential oil (water-soluble essential oil) prepared in the comparative example 4, and the essential oil needs to be subjected to filtration sterilization treatment and then to subsequent cell experiments;

(5) experimental group 3: the test reagent is the patchouli oil prepared in the example 1, 0.84ml of water and 400 μ l of SF410 are added into 100 μ l of patchouli oil, after uniform mixing, the patchouli oil is diluted by one tenth of sterilized double distilled water to obtain the test reagent, and the test reagent needs to be filtered and sterilized firstly and then is subjected to subsequent cell experiments;

(6) experimental group 4: the test reagents are the patchouli oil and the bay oil prepared in the example 1, the patchouli oil and the bay oil are mixed according to the ratio of 1:1.3 to obtain mixed oil, then 0.19ml of water and 920 μ l of SF410 are added into 230 μ l of the mixed oil, after the mixed oil is mixed uniformly, the mixed oil is diluted by one tenth of sterilized double distilled water to obtain the test reagent, the test reagent needs to be filtered and sterilized firstly, and then the subsequent cell experiment is carried out;

(7) experimental group 5: the test reagents were patchouli oil and bay oil prepared in comparative example 1, and were prepared as in (6);

(8) experimental group 6: the test reagents were patchouli oil and bay oil prepared in comparative example 2, prepared as in (6);

(9) experimental group 7: the test reagents were patchouli oil and bay oil prepared in comparative example 3, prepared as in (6);

(10) experimental group 8: the test reagents were patchouli oil and bay oil prepared in comparative example 5, prepared as in (6);

(11) experimental group 9: the test reagent is the bay oil prepared in example 1, 0.69ml of water and 520 ul of SF410 are added into 130 ul of bay oil, after uniform mixing, the bay oil is diluted by one tenth of sterilized double distilled water to obtain the test reagent, and the test reagent needs to be filtered and sterilized;

(12) blank control group: the test reagent is sterilized double distilled water which is not incubated with H1N1 influenza virus diluent, and 20 mul of sterilized double distilled water is directly added into MDCK single-layer cells.

The cell used in this experimental example was a dog kidney passaged cell (MDCK), and the virus strain used was the H1N1 influenza virus strain. Firstly, a test reagent is mixed with H1N1 influenza virus diluent (with the titer of 2)⁴HAU) was mixed (volume ratio 1: 1) to obtain a mixed solution, and incubated at 37 ℃ for 2 hours. Then 20. mu.l of the mixture was added to MDCK monolayer cells (MDCK cell inoculation as 1X 10⁴One well was inoculated in a 96-well plate, and a MEM medium containing 5% bovine serum was added to culture until MDCK monolayer cells were formed), and after 1 hour of infection, 100. mu.l of a MEM medium containing 5% bovine serum was added and placed in an incubator to culture for 24 hours. After the experiment is finished, the survival rate of the MDCK cells is detected by a CCK-8 method. Discarding the culture medium from the cells in the 96-well plate, adding CCK-8 solution (using the culture medium to dilute CCK-8 stock solution to be one percent of the stock solution), incubating for 2h, and using an enzyme-labeling instrument at 5 DEGThe OD value of each well was measured at 70nm, and the cell viability was calculated from the OD value.

Table 1: results of Virus inhibition experiments (3 replicates per experimental group)

The results are shown in table 1 and analyzed as follows:

the data of the negative control group show that the influenza virus used in the experiment can effectively infect MDCK cells, resulting in the reduction of the survival rate of the MDCK cells. The data of the positive control show that the commonly used drug ribavirin can effectively inhibit influenza virus and reduce the killing effect of the influenza virus on MDCK cells, and the ribavirin with the concentration does not show obvious cytotoxicity. The cells of the blank control group are not invaded by influenza virus, the cell activity is high, the survival rate is high, and the cells used in the experiment are proved to have higher activity. In the blank control group, the survival rate of MDCK cells was greatly affected by the invasion of influenza virus compared with the negative control group.

The test group 4 used the patchouli oil and bay oil prepared in example 1, and the test agent of test group 4 had a stronger inhibitory effect on influenza virus than those of test groups 9 and 3, so that the killing effect of influenza virus on MDCK cells was reduced. From the data of experimental group 3, it is clear that the patchouli oil alone has a certain effect on the inhibition of influenza virus, but its effect is not very prominent compared to the positive control ribavirin. As can be seen from experiment group 9, the effect of inhibiting influenza virus by using laurel oil alone is very weak and is basically equal to the effect of double distilled water (see negative control), but experiment group 4 adds laurel oil into the patchouli oil, and the laurel oil can enhance the effect of inhibiting influenza virus by the patchouli oil.

Experimental group 5 used the patchouli oil and the bay oil prepared in comparative example 1, and since the bay oil of this group was obtained by directly distilling and extracting bay leaf powder, the bay oil had a less potent antiviral effect on patchouli than the bay oil prepared in example 1. In the experimental group 6, the laurel leaf powder cold-soaked with ethyl acetate was not subjected to the enzymatic treatment, but the obtained laurel oil was not much different in the efficacy of promoting the antiviral ability of patchouli, compared with the laurel oil of example 1. However, the yield of the bay oil is significantly reduced during the extraction process. In the experimental group 7, the effect of the bay oil on promoting the antiviral ability of the patchouli is reduced compared with the bay oil of the example 1. Comparative example 3 the bay leaf powder was not soaked with ethyl acetate, resulting in the bay oil finally obtained containing more impurities (referring to the components not having the efficacy of promoting the antiviral ability of patchouli). In the finally obtained laurel oil, the content of effective components (components with effect of promoting antiviral ability of herba Cisii) is reduced, resulting in weakening effect of laurel oil. Experimental group 8 using the bay oil prepared in comparative example 5, the bay leaf powder was treated with ethyl acetate for a long time, resulting in loss of part of the functional ingredients (i.e., ingredients having the effect of promoting the antiviral ability of patchouli), and the content of the functional ingredients was reduced in the finally obtained bay oil. The ethyl acetate cold-soaking treatment of the laurel leaf powder for a certain time can enable partial substances in the laurel leaf powder to be leached out, and the partial substances do not have synergistic effect on the patchouli oil. However, the long time of the cold soaking treatment can cause a great amount of substances in the bay leaf powder to be dissolved out, and the substances also comprise functional components with synergistic enhancement effect on the patchouli, so the time of the cold soaking treatment is a relatively critical factor, and the proper time of the cold soaking treatment can ensure that the bay oil contains a relatively large amount of target functional components (components capable of forming synergistic enhancement effect on the patchouli oil) and can also reduce the existence of impurities (components incapable of forming synergistic enhancement effect on the patchouli) as much as possible.

The experiment group 1 uses the full-component essential oil in the blasting beads, and all components have synergistic effect, so that the influenza virus inhibitor has a good influenza virus inhibition effect. In the experimental group 2, the bay oil is absent from the essential oil, and the effect of the essential oil on inhibiting influenza viruses is weakened, which shows that although the bay oil has weak antiviral effect, the bay oil has the effect of promoting the essential oil formula to resist the influenza viruses.

The foregoing is merely an example of the present invention and common general knowledge of known specific structures and features of the embodiments is not described herein in any greater detail. It should be noted that, for those skilled in the art, without departing from the structure of the present invention, several changes and modifications can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.

Claims

1. The utility model provides an essential oil gauze mask, includes the gauze mask body, its characterized in that still including setting up at this internal pearl that explodes of gauze mask, it includes the shell that can break to explode the pearl, the parcel has essential oil in the shell, essential oil includes the composition of following part by weight: 160 portions of tea tree oil, 140 portions of cnidium fruit oil, 120 portions of magnolia oil, 80 to 120 portions of patchouli oil, 140 portions of blumea oil, 180 portions of elsholtzia oil, 80 to 120 portions of rosemary oil, 160 portions of bay oil, 80 to 120 portions of geranium oil, 150 portions of peppermint oil and 150 portions of laggera oil.

2. The essential oil mask of claim 1, wherein the magnolia oil is prepared by supercritical carbon dioxide extraction.

3. The mask containing essential oils of claim 2, wherein the tea tree oil, cnidium officinale oil, patchouli oil, mugwort leaf oil, elsholtzia oil, rosemary oil, bay oil, geranium oil, peppermint oil and laggera oil are prepared by steam distillation.

4. An essential oil mask as claimed in claim 3, wherein the patchouli oil is extracted from patchouli of the genus Musk of the family Scrophulariaceae.

5. The essential oil mask of claim 4 wherein the lauric oil is prepared by the following method: crushing fresh bay leaves to obtain bay leaf powder, carrying out cold leaching on the bay leaf powder by using ethyl acetate, and then filtering to obtain a solid phase to obtain a material A; performing enzymolysis treatment on the material A by using cellulase to obtain a material B; and (3) extracting the essential oil in the material B by using a steam distillation method to obtain the bay oil.

6. The mask containing essential oils of claim 5, wherein the ratio of the bay leaf powder to the ethyl acetate is 1g (5-9) ml, and the bay leaf powder is treated by cold soaking with ethyl acetate for 1-2 h.

7. The essential oil mask as claimed in claim 6, wherein the material B is obtained by the following method: adding water and cellulase into the material A to obtain a system A; obtaining a system B after the system A is subjected to enzymolysis reaction; the material B is a solid phase in the system B.

8. The essential oil mask as claimed in claim 7, wherein the content of cellulase in the system A is 1-2 wt%, the time of enzymolysis reaction is 2-3h, the temperature of enzymolysis reaction is 35-40 ℃, and the pH value of enzymolysis reaction is 7.0-8.0.

9. The oil mask according to any one of claims 1 to 8, wherein the size of the exploded bead is 4.5 to 5.0 mm.

10. The method for manufacturing an essential oil mask according to claim 9, comprising the steps of: