CN111248218A - Composition for treating common weeds in paddy field by using mequintocet-mexyl and penoxsulam and application of composition - Google Patents
Composition for treating common weeds in paddy field by using mequintocet-mexyl and penoxsulam and application of composition Download PDFInfo
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- CN111248218A CN111248218A CN202010199317.8A CN202010199317A CN111248218A CN 111248218 A CN111248218 A CN 111248218A CN 202010199317 A CN202010199317 A CN 202010199317A CN 111248218 A CN111248218 A CN 111248218A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/38—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< where at least one nitrogen atom is part of a heterocyclic ring; Thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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Abstract
The invention discloses a composition for preventing and killing common weeds in a paddy field by using mequindox and penoxsulam and application thereof. The herbicide composition can effectively prevent and kill off gramineous and broadleaf weeds in the paddy field, and is safe to paddy rice; the dosage can be obviously reduced, the application times can be reduced, and the chemical prevention and control cost in the field can be reduced; the generation and reoccurrence of the drug resistance of the weeds are delayed; expanding the weed control spectrum of the herbicide and the like. Is a feasible herbicide composition for preventing and killing common weeds in paddy fields at present.
Description
Technical Field
The invention belongs to the technical field of plant protection, and relates to a herbicide composition taking mequindox and penoxsulam as effective components, which is used for preventing and killing off common gramineae and broadleaf weeds in paddy fields.
Background
Rice is the first major food crop in the world, rice is the staple food for 40% of the world population, and rice is regarded as important food for 20% of the world population. Asian rice planting area and total rice yield are located in the first world and account for 90% of the world, wherein the country with the largest rice planting area is India and China is the second country; the country with the highest total rice yield is China. The total yield of rice is the first of three main grain crops of rice, wheat and corn in China, is the first grain crop and grain variety in China, and plays a significant role in grain production in China.
The weeds in the paddy field are various, the weed phase is extremely complex, and the weeds cannot be completely prevented and removed by using a single agent, so that the weeds in the paddy field need to be compounded for weeding. The conventional compound agent after rice seeding and before weed emergence comprises a series of soil compound agents such as butachlor-prometryn, butachlor-oxadiazon, butachlor-bensulfuron, pretilachlor-pyrazosulfuron, bensulfuron-methyl and mefenacet. The conventional compound agent after the emergence of weeds comprises a series of stem and leaf compound agents such as penoxsulam cyhalofop-butyl, cyhalofop-butyl dichloroquine phosphoric acid, bensulfuron-methyl dichloroquine phosphoric acid, oxanilide cyhalofop-butyl and the like. However, because the herbicide has relatively long service life and relatively serious drug resistance to weeds due to too many times of administration, a new compound formula needs to be developed urgently to solve the problem of resistance in the field.
Mequindox belongs to a novel HPPD (hydroxyphenylpyruvate dioxygenase) herbicide, is a post-emergence stem and leaf treatment agent, and after weeds are absorbed by stems and leaves, the process of converting hydroxyphenylpyruvate into homogentisic acid is hindered by inhibiting the activity of in-vivo HPPD, so that tocopherols and plastoquinone cannot be normally synthesized, the synthesis of carotenoid in a target body is influenced, the leaves are whitened, the growth of weeds is hindered, the weeds are killed, and common gramineous weeds and part of broadleaf weeds in paddy fields can be effectively prevented and removed.
Penoxsulam is a triazolopyrimidine sulfonamide herbicide that acts by inhibiting acetolactate synthase (ALS). The mechanism of action of penoxsulam is to prevent cell division and plant growth by inhibiting the biosynthesis of the essential amino acids leucine, isoleucine and valine in plants. Penoxsulam is effective in controlling barnyard grass (including barnyard grass resistant to propanil, quinclorac and acetyl-CoA carboxylase), annual Cyperaceae weeds, and broad-leaved weeds such as Marsh isopistil, Cyprinus carpioides, sesbania, Japanese chikusetsush, and monochoria vaginalis. The lasting period is as long as 30-60 days, and the harm of weeds in all seasons can be basically controlled by one-time application. Meanwhile, the herbicide can also prevent and kill bensulfuron methyl weeds in rice fields, and has residual activity on a plurality of broadleaf and cyperaceae weeds, barnyard grass and the like.
Disclosure of Invention
The invention aims to prevent and kill the weeds in the paddy field by the herbicide composition.
The invention realizes the aim through the following technical scheme:
the herbicide composition has the main components of mequintocet-mexyl and penoxsulam and is used for preventing and killing gramineous weeds and broadleaf weeds in paddy fields.
The medicament using method comprises the following steps: and (3) carrying out mechanical transplanting on rice fields and transplanting rice fields, carrying out weed 2-3 leaf stage, and carrying out spray treatment on stem leaves.
Compared with the existing common herbicide for paddy fields, the herbicide composition has the following advantages:
(1) has obvious synergistic effect on gramineous weeds such as barnyard grass and broadleaf weeds such as snakehead intestine, and the control effect is obviously better than that of single agent when applied independently.
(2) Can obviously reduce the dosage, reduce the dosage frequency and reduce the chemical preventing and removing cost in the field.
(3) Can delay the generation and reoccurrence of drug resistance of weeds.
Drawings
FIG. 1 of the accompanying drawings shows the composition of mequindox and penoxsulam p-barnyard grass ED90And (3) an equivalence line graph, wherein a coordinate point on the curve, which is farthest away from the theoretical equivalent line segment, is the optimal proportion of the composition of the mequintocet-mexyl and the penoxsulam for preventing and killing barnyard grass. FIG. 2 shows the composition of mequindox and penoxsulam versus snakehead intestine ED90And (3) an equivalence line graph, wherein a coordinate point on the curve, which is farthest away from a theoretical equivalent line segment, is the optimal proportion of the composition of the mequindox and the penoxsulam for preventing and killing the eclipta prostrata. FIG. 3 shows the composition of mequindox and penoxsulam versus total grass ED90And (3) an equivalence line graph, wherein a coordinate point on the curve, which is farthest away from a theoretical equivalent line segment, is the optimal proportion of the mequindox and penoxsulam composition to control the total weeds.
Detailed Description
(I) screening test-indoor test for the composition ratio of mequindox and penoxsulam:
the laboratory tests were carried out in a sunlight greenhouse from 1 month 2020 to 2 months 2020.
Reagent to be tested: (ii) mequintocet; penoxsulam
The tested weeds: barnyard grass (seeds were collected from Jiangpu farm in Nanjing, Jiangsu province in 2018 in 9 months).
Snakehead intestines (seeds are collected from the river, Su province, salt city, Tezhuang in 2017 in 9 months).
Laboratory test procedure
1. Medicament dose setting for screening test of composition ratio of mequindox and penoxsulam
1.1 Each single dose setting is as follows:
mequindox (a): 0(A0), 0.5(A1), 1(A2)2(A3), 4(A4) g a.i./hm2;
Penoxsulam (B): 0(B0), 3.75(B1), 7.5(B2), 15(B3), 30(B4) g a.i./hm2。
1.2 screening test drug dose settings for the composition ratio of mequindox and penoxsulam as shown in table 1:
TABLE 1 screening dose setting table for composition ratio of mequindox and penoxsulam
2. Screening test process for composition ratio of mequindox and penoxsulam
A mixed soil with a soil-fertilizer ratio of 2: 1, a pH of 6.1, an organic matter content of 1.4% and no herbicide used, was prepared, and the mixed soil was filled in a small plastic box with a specification of 7X 7cm and a hole at the bottom, and water was added until saturated. Selecting plump and dormancy-released barnyard grass, sowing, and growing in a sunlight-controlled greenhouse at 30 deg.C under dark condition at 25 deg.C for 12: 12h (D: L). After the seedlings of the weeds emerge regularly, the seedlings are fixed, and each pot is fixed to 12 plants. Culturing after final singling, and spraying stem and leaf when the weed grows to 2-3 leaves. The spraying adopts a 3WP-2000 type walking type bioassay spraying tower produced by Nanjing agricultural mechanization research institute of agricultural department, the rotating speed of a main shaft is 96mm/r, the spraying height is 300mm, the effective spraying width of a nozzle is 350mm, the flow rate of the nozzle is 390mL/min, the walking distance is 1340mm, and the volume of liquid medicine is 30 mL. After treatment, the seeds are continuously put into a sunlight greenhouse for growth.
After 21 days of medication, investigating and recording the fresh weight of weeds in each plastic box, evaluating the combined weed suppression effect of the mequindox and the penoxsulam by an equivalent line method, and calculating the fresh weight suppression rate of each dose combination of the single dose of the mequindox, the penoxsulam and the mequindox and the penoxsulam to the weeds.
Fresh weight inhibition ratio (% control fresh weight-treated fresh weight)/control fresh weight × 100
Then DPS software is used for carrying out professional statistics to obtain 90 percent dosage (namely ED) of methyl quintocet-mexyl and penoxsulam for inhibiting the proparase barnyard grass and eclipta prostrata90Value), ED of penoxsulam to inhibit weeds at each dose treatment of mequindox90Sulfoxaflor, and ED of mequintocet-inhibiting weeds at each dose treatment of penoxsulam90The value is obtained. The dose of the mequindox is used as an abscissa, the dose of the penoxsulam is used as an ordinate, and ED of the two single doses on weeds is marked on a coordinate graph90The coordinate points of the values are connected in parallel to form a line segment which is used as an equivalent line of the theoretical mixture of the two herbicides, namely the mequintocet-mexyl and the penoxsulam. The ED of penoxsulam at each dose of mequindox and each dose of mequindox is then plotted on a graph90Values for the coordinate points, ED of mequintocet-mefenacet at each dose of penoxsulam and penoxsulam90And (4) simulating a curve by using coordinate points formed by the values. If these coordinate points are below the theoretical equivalent line, the effect is synergistic, above that the antagonism is obtained, and the addition is obtained when the coordinate points are close to the theoretical equivalent line. Finding out a coordinate point which is farthest away from the theoretical equivalent line segment on the curve, namely the optimal proportion of the composition of the mequindox and the penoxsulam for preventing and killing the weeds in the paddy field.
The results of the laboratory tests are shown in the following table:
TABLE 2 fresh weight inhibition of Echinochloate by Methoquintocet-mefone and penoxsulam compositions (%)
As can be seen from the table 2 and the attached figure 1 in the specification, both the mequindox and the penoxsulam have high activity to the original variety barnyard grass, and the combination of the mequindox and the penoxsulam has a synergistic effect on the barnyard grass. As shown in the table 2, the fresh weight inhibition effect of the composition of the mequintocet-mefenacet and the penoxsulam in different proportions on barnyard grass is that the dose of the mequintocet-mefenacet is 2-4 g of a.i./hm2The dosage of the penoxsulam is 15-30 g a.i./hm2When the fresh weight inhibition rate of barnyard grass reaches or approaches to 100 percent, while the composition of the mequintocet-mexyl and the penoxsulam in the attached figure 1 of the specification has ED the barnyard grass90As can be seen from the isobologram, according to the evaluation of the isobologram method, the optimal proportion of the combination of the mequindox and the penoxsulam for controlling the barnyard grass is 1.5: 10 in theory. Therefore, the results of the laboratory test are combined, and when the dosage of the mequintocet is 2g a.i./hm2The dosage of the penoxsulam is 14-16 g a.i./hm2In this case, gramineous weeds in paddy fields having barnyard grass as the dominant species can be effectively prevented.
TABLE 3 fresh weight inhibition of crab grass by mequindox and penoxsulam compositions (%)
As can be seen from the table 3 and the attached figure 2 of the specification, both the methyl quinclomazone and the penoxsulam have higher activity on the eclipta prostrata, and the combination of the methyl quinclomazone and the penoxsulam has a synergistic effect on the eclipta prostrata. The inhibition effect of the compositions of the methyl quintocet-mexyl and the penoxsulam with different proportions in the table 3 on the fresh weight of the snakehead intestine is known, and the amount of the methyl quintocet-mexyl is 2-4 g of a.i./hm2The dosage of the penoxsulam is 15-30 g a.i./hm2When the inhibition rate of the composition on the fresh weight of the snakehead gut reaches or approaches 100 percent, the composition of the methyl quintocet-mexyl and the penoxsulam shown in the attached figure 2 of the specification has ED on the snakehead gut90According to the evaluation of an equivalent line method, the optimal proportion of the combination of the mequindox and the penoxsulam for controlling the snakehead intestine is 1: 10 theoretically. Therefore, the results of the laboratory test are combined, and the Mequintocet-mexylThe dosage of ketone is 2g a.i./hm2The dosage of the penoxsulam is 20g a.i./hm2In this way, the broadleaf weeds in the paddy field with the snakehead gut as the dominant species can be effectively prevented and killed.
TABLE 4 fresh weight inhibition of Total grass (%) -by Methoquintocet-mefone and penoxsulam compositions
As can be seen from the table 4 and the attached figure 3 in the specification, the mequindox and the penoxsulam have higher activity on the total grass, and the combination of the mequindox and the penoxsulam has a synergistic effect on the total grass. As shown in the table 4, the fresh weight inhibition effect of the composition of the mequintocet-mefenacet and the penoxsulam in different proportions on the total grass is known, and the amount of the mequintocet-mefenacet is 2-4 g a.i./hm2The dosage of the penoxsulam is 15-30 g a.i./hm2When the total grass fresh weight inhibition rate reaches or approaches 100 percent, the total grass ED is treated by the composition of the mequindox and the penoxsulam shown in the attached figure 3 of the specification90According to the evaluation of an equivalent line method, theoretically, the optimal ratio of the combined control total grass of the mequindox and the penoxsulam is 1: 10, the safety of the mequindox to rice, the improvement of the control effect and other factors are comprehensively considered, and the optimal ratio of the combined control total grass of the mequindox and the penoxsulam to the weeds in the paddy field is 1: 10. Therefore, the results of the laboratory test are combined, and when the dosage of the mequintocet is 2g a.i./hm2The dosage of the penoxsulam is 20g a.i./hm2When the herbicide is used, the weeds in the paddy fields with barnyard grass and snakehead gut as dominant species can be effectively prevented and killed.
Safety test-laboratory test of (di) mequindox and penoxsulam composition on rice
The indoor experiments were carried out in a sunlight greenhouse from 1 month 2020 to 2 months 2020.
Reagent to be tested: (ii) mequintocet; penoxsulam
Test work: japonica rice (Xudao No. 9, 2016 available from Jiangsu province farm in 12 months)
Indica rice (Hanyou 70, 2016 in Jiangsu province farm college in 12 months)
Laboratory experiment process
1. Safety test medicament dose setting of mequindox and penoxsulam composition on rice
The safety test of the composition on rice the dose settings of the agents are as follows:
mequindox + penoxsulam: 0+0, 0.5+3.75, 1+7.5, 2+15, 4+30g a.i./hm2
2. Safety test process of composition of mequindox and penoxsulam for rice
Preparing mixed soil with a soil-fertilizer ratio of 2: 1, wherein the pH of the mixed soil is 6.1, the organic matter content is 1.4 percent, and the mixed soil is never used with herbicide, filling 4/5 deep soil in a plastic flowerpot, and absorbing water at the bottom until the soil is saturated with water. Respectively sowing indica rice (Ganyou 73) and japonica rice (Xudao No. 9) seeds in flowerpot uniformly, covering fine soil with thickness of 0.2-0.5cm after sowing, keeping soil moist, and culturing in greenhouse (temperature: 27 deg.C in daytime and 22 deg.C at night). A transparent plastic box 22cm long, 15cm wide and 10cm high is filled with soil (pH 5.6, organic matter content 1.4%), conditions of a mechanical transplanting field are simulated, and water is added to soak the soil. Transplanting japonica rice No. 9 and indica rice Hanyou 73 at the 3-leaf stage into a transparent plastic box, wherein 10 plants are planted in each box, and carrying out stem and leaf spraying treatment when the rice grows to the 4.5-leaf stage; simulating the condition of transplanting the paddy field, and adding water to soak the soil. Transplanting No. 9 nonglutinous rice and indica type dry rice 73 of 4-leaf stage japonica rice into a transparent plastic box, 10 plants in each box, and spraying stems and leaves when the rice grows to 5.5-leaf stage. The spraying adopts a 3WP-2000 type walking type bioassay spraying tower produced by Nanjing agricultural mechanization research institute of agricultural department, the rotating speed of a main shaft is 96mm/r, the spraying height is 300mm, the effective spraying width of a nozzle is 350mm, the flow rate of the nozzle is 390mL/min, the walking distance is 1340mm, and the volume of liquid medicine is 30 mL. After treatment, the seeds are continuously put into a sunlight greenhouse for growth.
And (3) investigating and recording the fresh weight of each treated rice 21 days after the application of the composition, analyzing the difference significance between different dosages by using DPS software, calculating the selectivity index of the composition, and evaluating the safety of the composition of the mequintocet-mefenacet and the penoxsulam on the rice according to the two aspects.
Fresh weight inhibition ratio (% control fresh weight-treated fresh weight)/control fresh weight × 100
The selectivity index is calculated as:
selectivity index (Z) versus ED for crop plants10ED of weeds90
TABLE 5 evaluation of safety of rice 21 days after drug administration (machine-transplanted japonica rice)
TABLE 6 evaluation of safety of rice 21 days after drug administration (transplanting japonica rice in field)
TABLE 7 evaluation of safety of rice 21 days after drug administration (transplanting field indica rice)
Note: the difference significance of the lower case letters is 0.05 level by adopting the Duncan new repolarization difference method of the DPS software
TABLE 8 Selectivity index of mequindox and penoxsulam compositions for rice
As can be seen from tables 5 to 7, the significance test is performed by the DPS software, the inhibition effect on the fresh weight of rice is not obviously increased with the increase of the dosages of the compositions of the mequintocet-mexyl and the penoxsulam, and the difference significance does not exist between the average fresh weight of each treatment and the average fresh weight of a control, and as can be seen from table 8, the selectivity index of the compositions of the mequintocet-mexyl and the penoxsulam on the japonica rice transplanted by a machine is 6.08, the selectivity index on the japonica rice transplanted by a transplanting field is 6.31, and the selectivity index on the indica rice transplanted by the transplanting field is 4.01 and is more than 4. Therefore, the composition of the mequindox and the penoxsulam is safe to the growth of rice in view of the safety evaluation and the selectivity index evaluation of the composition of the mequindox and the penoxsulam on the fresh weight of the indica rice.
Claims (7)
1. A composition for controlling common weeds in paddy fields by using mequintocet-mexyl and penoxsulam and application thereof are characterized in that gramineae weeds and broadleaf weeds in the paddy fields can be prevented and removed.
2. According to the claim 1, the composition for controlling common weeds in paddy fields by using mequintocet-mexyl and penoxsulam has the control effect on barnyard grass and carignane of more than 90 percent under a proper dosage proportion.
3. The composition of claim 1, wherein the composition is safe for rice and free of phytotoxicity at the experimentally set dosage.
4. According to the claim 1, compared with a single-agent application, the composition for controlling the weeds in the paddy field by the mequindox and the penoxsulam can expand the weed control spectrum, and can control both grassy weeds and broadleaf weeds.
5. The composition according to claim 1, wherein the dosage of mequintocet is 2-4 g a.i./hm2The dosage of the penoxsulam is 15-30 g a.i./hm2Within the range, namely when the ratio of the mequindox to the penoxsulam is 1: 10, the effect on preventing and killing weeds in the field is optimal.
6. The composition of claim 1, wherein the composition is prepared from mequintocet-mexyl and penoxsulam as effective components and any one of the agriculturally acceptable adjuvants.
7. The use as claimed in claim 1, wherein the composition for controlling weeds in paddy fields by mequindox and penoxsulam is used by spraying stem leaves.
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CN102669119A (en) * | 2009-08-28 | 2012-09-19 | 北京燕化永乐农药有限公司 | Herbicide composition containing penoxsulam |
CN101731232A (en) * | 2010-01-13 | 2010-06-16 | 刘雪源 | Weed control foliar herbicide compositions in paddy field |
CN104557739A (en) * | 2013-10-25 | 2015-04-29 | 华中师范大学 | Triketone compound and preparation method and application thereof |
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