CN111218491A - Steam-ammonia combined pretreatment process for improving lignocellulose conversion efficiency - Google Patents
Steam-ammonia combined pretreatment process for improving lignocellulose conversion efficiency Download PDFInfo
- Publication number
- CN111218491A CN111218491A CN201811427613.8A CN201811427613A CN111218491A CN 111218491 A CN111218491 A CN 111218491A CN 201811427613 A CN201811427613 A CN 201811427613A CN 111218491 A CN111218491 A CN 111218491A
- Authority
- CN
- China
- Prior art keywords
- pretreatment
- ammonia
- steam
- lignocellulose
- pretreatment process
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2201/00—Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a steam-ammonia combined pretreatment process for improving lignocellulose conversion efficiency, and belongs to the technical field of biorefinery. The process employs steam to pretreat the lignocellulose prior to ammonia pretreatment. The steam-ammonia combined pretreatment process can destroy hemicellulose and lignin structures in biomass, simultaneously convert partial xylan into xylooligosaccharide or xylose, and reduce the dosage of hemicellulase, thereby reducing the cost of converting lignocellulose into biological products and promoting the industrial large-scale production of lignocellulose conversion.
Description
Technical Field
The invention belongs to the technical field of biorefinery, and relates to a steam-ammonia combined pretreatment process for improving lignocellulose conversion efficiency.
Background
Lignocellulose is a renewable resource, is widely distributed and easily obtained, and can be used as a raw material for producing chemical products such as liquid fuel and the like. However, lignocellulose has a complex structure, wherein lignin and hemicellulose are wrapped by cellulose to form a compact structure, and polymers such as saccharides are connected together through hydrogen bonds or covalent bonds to form biodegradation resistance, so that the lignocellulose is difficult to biodegrade. The effective pretreatment mode can break the structure of the biomass and improve the biotransformation efficiency.
Currently, ammonia pretreatment is one of the popular pretreatment modes for converting lignocellulose into ethanol and other products. Ammonia Pretreatment can extract lignin from the interior of lignocellulose to the surface, increasing the porosity within the lignocellulose, and having less effect on the composition of the lignocellulose (Lau M J, Lau M W, Gunawan C, et al. Ammonia Fiber Expansion (AFEX) Pretreatment, enzyme Hydrolysis, and Fermentation on expression Panel FruitBlock Fiber (FBF) for cellulose Ethanol production. applied Biochemistry & Biotechnology,2010,162(7): 1847-1857.). Meanwhile, the ammonia can be recycled, and the residual ammonia can be used as a nitrogen source of the fermentation strain. However, the ammonia pretreatment has the limitation that the structure of hemicellulose is slightly influenced, and a large amount of hemicellulases such as xylanase, pectinase and the like are required to be supplemented to convert the hemicellulose into monosaccharide, so that the cost of the whole biotransformation is increased.
In addition, steam pretreatment is also a conventional pretreatment method, and the pretreatment is mainly to treat lignocellulose by using high-temperature and high-pressure steam. Under the action of high temperature, water vapor penetrates into the interior of the lignocellulosic feedstock, converting the ethylphthaloyl groups and some other functional groups in the feedstock into organic acids, hydrolyzing hemicellulose into oligosaccharides, while softening and degrading part of the lignin (Kim S M, Dien B S, Tumbleson M E, et al. improvement of substrate from cornstover using sequential water heater technology,2016,216: 706-713.). The lignocellulose is treated by utilizing steam pretreatment, so that the structure of the lignocellulose is more fluffy, and part of xylan is converted into xylan or xylose, thereby being more beneficial to the enzyme hydrolysis. However, to achieve a better pretreatment effect, the steam pretreatment needs a higher temperature, and the generated series of degradation products have significant inhibition effects on subsequent enzymolysis and fermentation, which is not beneficial to the industrial application of cellulosic ethanol.
Disclosure of Invention
The invention aims to provide a steam-ammonia combined pretreatment process for improving lignocellulose conversion efficiency, which combines steam pretreatment and ammonia pretreatment, omits a step of supplementing hemicellulase in the ammonia pretreatment, improves xylose conversion rate and reduces enzyme usage.
The technical scheme for realizing the purpose of the invention is as follows:
the steam-ammonia combined pretreatment process for improving the lignocellulose conversion efficiency comprises the following steps:
the method comprises the steps of firstly carrying out steam pretreatment on a lignocellulose raw material at 160-250 ℃, evaporating moisture in the raw material to be 20% -80% of the weight of a substance after complete reaction, introducing or adding ammonia, and carrying out ammonia pretreatment at 80-160 ℃.
The lignocellulose is conventionally used lignocellulose, and can be wheat straw, corn straw, agricultural and forestry waste, rice straw, sorghum straw, soybean straw, forestry waste, recycled wood pulp fiber, wood chips, softwood, hardwood or animal manure.
The lignocellulose raw material adopts conventionally used lignocellulose raw material, and can be long-strip lignocellulose, blocky lignocellulose or granular lignocellulose, the length of the long-strip lignocellulose is 0.01 mm-10 cm, and the blocky density of the blocky lignocellulose is 50-900 kg/m3The diameter of the granular lignocellulose is 0.5 mm-10 cm.
Preferably, the mass concentration of the lignocellulose raw material in the pretreatment process is 10-60%.
The steam pretreatment can be steam explosion pretreatment, hot water pretreatment or weak acid pretreatment.
Preferably, the steam pretreatment time is 5-120 min.
The ammonia is selected from ammonia water solution, liquid ammonia or ammonia gas.
Preferably, the ammonia pretreatment time is 10-120 min.
The invention also provides a fermentation process for improving the conversion rate of cellulose ethanol based on the pretreatment process, which comprises the following steps:
step 1, pretreatment: pretreating lignocellulose raw materials by steam at 160-250 ℃, evaporating moisture in the raw materials to be 20-80% of the weight of the materials after the reaction is completed, introducing or adding ammonia, and pretreating the ammonia at 80-160 ℃;
step 3, fermentation: adding fermentation strain, and fermenting to obtain fermented product.
In step 2, the hydrolase is selected from enzymes conventionally used in a lignocellulose fermentation process, and may be cellulase and/or hemicellulase, such as one or a combination of pectinase and xylanase.
In the step 3, the fermentation strain is a strain which is conventionally used in a lignocellulose fermentation process and can be yeast, bacteria, mould and the like.
In step 3, the fermentation product obtained may be bulk chemicals, fine chemicals or animal feed, such as ethanol, butanol, acetone, acetic acid, lactic acid, aliphatic hydrocarbons, fats and oils, proteins, amino acids, etc., depending on the fermentation strain.
Compared with the prior art, the invention has the following advantages:
(1) the invention combines the steam pretreatment and the ammonia pretreatment, thereby not only effectively reducing the reaction intensity of the two pretreatments and reducing the energy consumption, but also increasing the yield of the conversion of the cellulose and the hemicellulose into the sugar.
(2) The steam pretreatment has no pollution to the environment and no waste liquid discharge, the ammonia gas in the ammonia pretreatment can be recycled, and the ammonia remained in the raw material can be used as a nitrogen source of a fermentation strain for the growth of microorganisms, thereby reducing the cost of additional nutrient substances and chemical reagents.
(3) The steam pretreatment effectively converts xylan in hemicellulose into xylooligosaccharide or xylose, increases pores inside cellulose, is beneficial to contact between enzyme and cellulose, and the steam also destroys complex chemical bonds between the cellulose and lignin, so that the ammonia can more easily extract the lignin inside the lignocellulose to the surface of the lignocellulose.
(4) The acidic inhibitor generated after the steam pretreatment can react with ammonia during the ammonia pretreatment, so that the toxicity of the inhibitor is reduced, and meanwhile, the ammonia pretreatment further destroys lignocellulose.
(5) During pretreatment, the concentration of inhibitors generated by steam and ammonia pretreatment is lower than that generated by dilute acid and dilute alkali pretreatment, and the pretreatment is favorable for enzyme hydrolysis and fermentation. The process can further reduce the production cost of enterprises and promote the industrial large-scale production of lignocellulose conversion.
Drawings
FIG. 1 is a graph of the results of the 24 hour enzymatic hydrolysis of sugar concentrations in example 1 with SAP and LHW, respectively, pretreated corn stover.
FIG. 2 is a graph showing the effect of xylanase presence on enzymatic hydrolysis during pretreatment of straw with SAP in example 2.
Detailed Description
In order to facilitate understanding of the invention, the invention will be described in more detail with reference to the drawings and preferred embodiments, but the scope of the invention is not limited to the following specific embodiments.
All terms of art used hereinafter have the same meaning as commonly understood by one of ordinary skill in the art, unless otherwise defined. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the scope of the present invention.
Unless otherwise specifically stated, reagents, starting materials, instruments, equipment and the like used in the present invention are commercially available or prepared by an existing method.
The following abbreviations are used in the examples:
SAP: combined Steam-Ammonia Pretreatment (Steam-Ammonia Pretreatment), LHW: hot water pretreatment (liquidportwater).
Example 1
The comparison of the enzymatic hydrolysis effects of corn stover pretreated with SAP and LHW, respectively, includes the following steps:
1. preparing raw materials: collecting corn stalks, naturally airing, and crushing into 1-4cm particles.
2.1SAP pretreatment: placing pulverized corn stalk in a reaction kettle, with dry matter concentration of 20% (based on total mass), heating to 160 deg.C, and reacting for 50 min. And opening the kettle cover immediately after the reaction is finished, naturally cooling to room temperature, reducing the water content of the straws to 60 percent (based on the dry weight of the straws), introducing ammonia gas (the mass ratio of the ammonia gas to the dry matter is 1: 1), heating to 110 ℃, and reacting for 5 min.
2.2LHW pretreatment: placing the crushed corn straws into a reaction kettle, raising the temperature to 200 ℃ when the dry matter concentration is 10 percent (based on the total mass), reacting for 5min, and naturally cooling to room temperature after the reaction is finished.
3. Respectively putting the pretreated straws into an enzymatic hydrolysis reactor, adding hydrolase and a citric acid-sodium citrate buffer solution with the mass concentration of 3% into the enzymatic hydrolysis reactor, carrying out enzymatic hydrolysis reaction in a shaking box (250rpm) at 50 ℃, and carrying out enzymatic hydrolysis for 24 hours.
The effect of enzymatic hydrolysis at 3% dry matter for SAP pretreatment and LHW pretreatment is shown in figure 2.
Represents the concentration of 3 percent of corn stalk enzyme hydrolysis glucose,
represents the concentration of 3% corn stalk enzyme hydrolysis xylose. As can be seen from FIG. 2, the glucose concentration of the corn stalks after 24 hours of enzymatic hydrolysis can reach 8.3g/L after the corn stalks are subjected to SAP pretreatment, while the glucose concentration of the corn stalks after 24 hours of enzymatic hydrolysis is only 6.6g/L after the corn stalks are subjected to LHW pretreatment, and the SAP pretreatment is 1.7g/L higher than LHW enzymatic hydrolysis glucose concentration, which indicates that the SAP pretreatment improves the conversion rate of glucose compared with the conventional hot water pretreatment. The concentration of xylose in the corn straws pretreated by SAP is 3.4g/L after 24 hours of enzymatic hydrolysis, and the concentration of xylose in the straws pretreated by LHW is 3.3 g/L.
Example 2
The influence of the addition or non-addition of xylanase on the enzymatic hydrolysis effect of SAP pretreatment corn straws comprises the following steps:
1. preparing raw materials: after the corn straws are collected, the corn straws are naturally dried and then crushed into particles of 1-4 cm.
2.1SAP pretreatment: placing pulverized corn stalk in a reaction kettle, with dry matter concentration of 20% (based on total mass), heating to 160 deg.C, and reacting for 50 min. And opening the kettle cover immediately after the reaction is finished, naturally cooling to room temperature, reducing the water content of the straws to 60 percent (based on the dry weight of the straws), introducing ammonia gas (ammonia gas: dry matter is 1: 1), heating to 110 ℃, and reacting for 5 min.
2.2 the pretreated stalks are placed in an enzyme hydrolysis reactor with a substrate mass concentration of 3%, added with hydrolase and citric acid-sodium citrate buffer solution, subjected to enzymolysis reaction in a shaking box (250rpm) at 50 ℃, added with 40mg of protein/g of glucan blue biological enzyme (cellulase protein: xylosidase protein ═ 1: 1) and 20mg of protein/g of glucan blue cellulase (cellulase only, no xylanase), and subjected to enzyme hydrolysis for 24 hours.
The effect of enzymatic hydrolysis of SAP pretreatment at 3% dry matter is shown in FIG. 2.
Represents the concentration of 3 percent of corn stalk enzyme hydrolysis glucose,
represents the concentration of 3% corn stalk enzyme hydrolysis xylose. As can be seen from FIG. 2, after the corn straws are pretreated by the SAP, 40mg of protein/g of glucan of the blueblue bio-enzyme (the protein of the cellulase: the protein of the xylosidase is 1: 1), the glucose concentration can reach 8.3g/L after 24 hours of enzymatic hydrolysis, 20mg of protein/g of glucan of the blueblue cellulase (only cellulase and no xylanase), and the glucose concentration is 7.8g/L after 24 hours of enzymatic hydrolysis, which indicates that the SAP pretreatment has little influence on the cellulase hydrolysis effect due to the reduction of the bio-enzyme content (containing cellulase and xylanase). SAP pretreatment of corn straw, enzyme amount: ultramarine bio-enzyme (protein of cellulase: protein of xylosidase ═ 1: 1) at 40mg protein/g glucan, xylose concentration 3.4g/L for 24 hours of enzymatic hydrolysis, and enzyme amount: the concentration of xylose after enzyme hydrolysis of 20mg protein/g glucan of ultramarine blue bio-enzyme (protein of cellulase: protein of xylanase ═ 1: 1) is 3.3g/L, which shows that SAP pretreatment and no influence on xylan conversion rate due to no addition of xylanase are achieved, and the process saves the use of xylanase.
Claims (10)
1. The steam-ammonia combined pretreatment process for improving the lignocellulose conversion efficiency is characterized by comprising the following steps of:
the method comprises the steps of firstly carrying out steam pretreatment on a lignocellulose raw material at 160-250 ℃, evaporating moisture in the raw material to be 20% -80% of the weight of a substance after complete reaction, introducing or adding ammonia, and carrying out ammonia pretreatment at 80-160 ℃.
2. The pretreatment process of claim 1, wherein said lignocellulosic biomass is selected from the group consisting of wheat straw, corn stover, agricultural and forestry waste, rice straw, sorghum straw, soybean stover, forestry waste, recycled wood pulp fiber, wood chips, softwood, hardwood, and animal manure.
3. The pretreatment process according to claim 1, wherein the lignocellulosic raw material is selected from the group consisting of elongated lignocelluloses, blocky lignocelluloses and granular lignocelluloses, the elongated lignocelluloses having a length of 0.01mm to 10cm, and the blocky lignocelluloses having a blocky density of 50 to 900kg/m3The diameter of the granular lignocellulose is 0.5 mm-10 cm.
4. The pretreatment process according to claim 1, wherein the mass concentration of the lignocellulosic feedstock during the pretreatment is in the range of 10% to 60%.
5. The pretreatment process of claim 1, wherein said steam pretreatment is selected from the group consisting of steam explosion pretreatment, hot water pretreatment, and weak acid pretreatment.
6. The pretreatment process according to claim 1, wherein the steam pretreatment time is 5-120 min.
7. The pretreatment process of claim 1, wherein the ammonia is selected from the group consisting of aqueous ammonia solution, liquid ammonia, and ammonia gas.
8. The pretreatment process according to claim 1, wherein the pretreatment time of ammonia is 10-120 min.
9. Fermentation process of the pretreatment process according to any one of claims 1 to 8, comprising the steps of:
step 1, pretreatment: pretreating lignocellulose raw materials by steam at 160-250 ℃, evaporating moisture in the raw materials to be 20-80% of the weight of the materials after the reaction is completed, introducing or adding ammonia, and pretreating the ammonia at 80-160 ℃;
step 2, hydrolysis: adding water and hydrolase into the pretreated material for hydrolysis;
step 3, fermentation: adding fermentation strain, and fermenting to obtain fermented product.
10. The fermentation process according to claim 9, wherein in step 2, the hydrolytic enzyme is selected from cellulase and/or hemicellulase, and the hydrolytic enzyme is selected from one or a combination of pectinase and xylanase; the fermentation strain is selected from yeast, bacteria or mould.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811427613.8A CN111218491A (en) | 2018-11-27 | 2018-11-27 | Steam-ammonia combined pretreatment process for improving lignocellulose conversion efficiency |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811427613.8A CN111218491A (en) | 2018-11-27 | 2018-11-27 | Steam-ammonia combined pretreatment process for improving lignocellulose conversion efficiency |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111218491A true CN111218491A (en) | 2020-06-02 |
Family
ID=70825664
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811427613.8A Pending CN111218491A (en) | 2018-11-27 | 2018-11-27 | Steam-ammonia combined pretreatment process for improving lignocellulose conversion efficiency |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111218491A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101824436A (en) * | 2009-03-06 | 2010-09-08 | 华东理工大学 | Improved method for preprocessing lignocellulose raw material |
| CN103993053A (en) * | 2014-05-07 | 2014-08-20 | 中国科学院广州能源研究所 | Method for pretreatment of biomass by water-ammonia coupling |
| WO2015175308A1 (en) * | 2014-05-13 | 2015-11-19 | Danisco Us Inc. | Improved enzymatic hydrolysis of biomass |
-
2018
- 2018-11-27 CN CN201811427613.8A patent/CN111218491A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101824436A (en) * | 2009-03-06 | 2010-09-08 | 华东理工大学 | Improved method for preprocessing lignocellulose raw material |
| CN103993053A (en) * | 2014-05-07 | 2014-08-20 | 中国科学院广州能源研究所 | Method for pretreatment of biomass by water-ammonia coupling |
| WO2015175308A1 (en) * | 2014-05-13 | 2015-11-19 | Danisco Us Inc. | Improved enzymatic hydrolysis of biomass |
Non-Patent Citations (2)
| Title |
|---|
| ELINA TAMPIO等: "Anaerobic digestion of autoclaved and untreated food waste", 《WASTE MANAGEMENT 》 * |
| 朱跃钊等: "木质纤维素预处理技术研究进展", 《生物加工过程》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Kumar et al. | Cellulosic and hemicellulosic fractions of sugarcane bagasse: Potential, challenges and future perspective | |
| Pensupa et al. | A solid state fungal fermentation-based strategy for the hydrolysis of wheat straw | |
| Madadi et al. | Recent status on enzymatic saccharification of lignocellulosic biomass for bioethanol production | |
| KR101036853B1 (en) | Process for the pre-treatment of lignocellulosic biomass by popping method combined with wet milling and process for the production of saccarification and bio-ethanol using the same | |
| US20100021980A1 (en) | Hybrid process for the production of biofuel | |
| ES2738514T3 (en) | Procedure for enzymatic hydrolysis of lignocellulosic material | |
| CN102304550B (en) | Method for producing ethanol or acetone and butanol by taking lignocellulose as raw material | |
| MX2012004072A (en) | Process for the preparation of a fermentation product from lignocellulose containing material. | |
| Han et al. | Production of sodium gluconate from delignified corn cob residue by on-site produced cellulase and co-immobilized glucose oxidase and catalase | |
| ES2703631T3 (en) | Procedures and systems for the saccharification of biomass | |
| WO2005079190A2 (en) | Production of ethanol and high-protein feed co-products from high-solids conversion of cereal grains and legumes | |
| Allen et al. | Lignocelluloses: an economical and ecological resource for bio-ethanol production-a review | |
| Devi et al. | Lignocellulolytic enzymes and bioethanol production from spent biomass of edible mushrooms using Saccharomyces cerevisiae and Pachysolen tannophilus | |
| JP5543359B2 (en) | Preparation of biothermal enhancer using Penicillium funiculosum enzyme | |
| WO2020206863A1 (en) | Methods for pre-treatment of lignocellulose by adding alkali or acid reagent during densification thereof and for biotransformation thereof | |
| Rana | Green approaches in the valorization of plant wastes: Recent insights and future directions | |
| CN107012176A (en) | A kind of method of the conversion production sodium gluconate by material, enzyme method of cellulose biomass | |
| Kumar et al. | Bioprocessing of cellulosic waste biomass for ethanol production by Chryseobacterium culicis Bp16 | |
| KR101055623B1 (en) | Biological pretreatment and saccharification method of lignocellulosic biomass and preparation method of bioethanol comprising same | |
| CN109971806A (en) | Mix lignocellulose pretreatment method and its zymotechnique | |
| CN111218491A (en) | Steam-ammonia combined pretreatment process for improving lignocellulose conversion efficiency | |
| CN111218490A (en) | Method for pretreating lignocellulose by using ammonia and auxiliary agent | |
| CN111218489A (en) | Method for pretreating lignocellulose by using ammonia and sulfonation reagent | |
| Valchev et al. | Use of enzymes in hydrolysis of maize stalks | |
| CN109913507A (en) | Synchronize the lignocellulosic method for refining for carrying out pretreatment and enzyme hydrolysis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |