CN111187358A - Preparation method of fig polysaccharide - Google Patents
Preparation method of fig polysaccharide Download PDFInfo
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- CN111187358A CN111187358A CN201811355555.2A CN201811355555A CN111187358A CN 111187358 A CN111187358 A CN 111187358A CN 201811355555 A CN201811355555 A CN 201811355555A CN 111187358 A CN111187358 A CN 111187358A
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- polysaccharide
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
The invention discloses a preparation method for extracting fig polysaccharide from fig, which is characterized by comprising the following steps: 1) pulverizing dried fructus fici, sieving with 80 mesh sieve, and collecting coarse powder; 2) mixing the fig dry powder with water and material in a ratio of 35: 1-55: 1 (mL/g) is added into distilled water, and the pH is adjusted to 7-9; 3) adding 0.05% -0.3% of cellulase, and performing ultrasonic extraction at the temperature of 50-110 ℃ for 2-4 h; 4) vacuum filtering the extractive solution, vacuum rotary evaporating the filtrate to volume of 10mL, adding 4 times of anhydrous ethanol, mixing, standing in 4 deg.C refrigerator for 12 hr, and centrifuging at 3000r/min for 10min to obtain crude polysaccharide; 5) passing the obtained crude polysaccharide through a macroporous adsorption resin column, eluting by using distilled water with the volume of 1-4 times of the column volume, eluting by using 30-80% ethanol, and collecting ethanol eluent; 6) recovering the reagent, and vacuum drying to obtain fig polysaccharide. The fig polysaccharide prepared by the invention has high product purity and good quality.
Description
Technical Field
The invention belongs to the technical field of medicines, and particularly relates to a preparation method of fig polysaccharide.
Background
Fig (ficuscarinica l.) belongs to ficus genus of moraceae family (Moracea), and its floret is hidden in receptacle, so that only false fruit formed by receptacle can be seen, and no flower can be seen, so that it is called "fig". The fig fruit has higher nutritive value and medicinal value, and is a special natural health-care fruit with homology of medicine and food. The fig is cool in nature and sweet in taste, has the effects of clearing heat, promoting fluid production, invigorating spleen, stimulating appetite, removing toxic substances and relieving swelling, and is used for treating diseases such as sore throat, intestinal heat constipation, dyspepsia and diarrhea. Ficus Carica Polysaccharide (FCPS) is an important functional factor of ficus carica, can significantly improve the immune function of mice, crucian carps and grass carps, and is a better immunomodulator. In addition, FCPS has a good ability to scavenge hydroxyl radicals, superoxide anion radicals, and 1, 1-diphenyl-2-trinitrophenylhydrazine (1, 1-diphenyl-2-piperidinylhydrazyl, DPPH) radicals in vitro, and is considered to be a naturally safe antioxidant.
At present, the extraction method of fig polysaccharide is reported at home and abroad, and the method combines ultrasonic-assisted cellulase-macroporous resin extraction and purification technologies to prepare the high-purity fig polysaccharide, so that the method is suitable for industrial production.
Disclosure of Invention
The invention aims to provide an extraction method of fig polysaccharide, and the fig polysaccharide has wide medicinal value.
The invention is completed by the following technical scheme, and the specific content comprises the following steps: method for extracting fig polysaccharide
1) Pulverizing dried fructus fici, sieving with 80 mesh sieve, and collecting coarse powder;
2) mixing the fig dry powder with water and material in a ratio of 35: 1-55: 1 (mL/g) is added into distilled water, and the pH is adjusted to 7-9;
3) adding 0.05% -0.3% of cellulase, and performing ultrasonic extraction at the temperature of 50-110 ℃ for 2-4 h;
4) vacuum filtering the extractive solution, vacuum rotary evaporating the filtrate to volume of 10mL, adding 4 times of anhydrous ethanol, mixing, standing in 4 deg.C refrigerator for 12 hr, and centrifuging at 3000r/min for 10min to obtain crude polysaccharide;
5) passing the obtained crude polysaccharide through a macroporous adsorption resin column, eluting by using distilled water with the volume of 1-4 times of the column volume, eluting by using 30-80% ethanol, and collecting ethanol eluent;
6) recovering the reagent, and vacuum drying to obtain fig polysaccharide.
The water-material ratio in the step 2) is 35: 1-55: 1 (mL/g).
Adding 0.05-0.3% of cellulase in the step 3), and carrying out ultrasonic treatment at 50-110 ℃ for 2-4 h.
The macroporous adsorption resin in the step 5) is one of JAD-1000, ZJX and HD-8 types.
The present invention will be further described with reference to specific embodiments, but the scope of the invention as claimed is not limited to the following embodiments.
The specific implementation mode is as follows:
example 1:
pulverizing dried fructus fici, sieving with 80 mesh sieve, and collecting coarse powder; mixing the fig dry powder with water and material in a ratio of 35: 1 (mL/g) was added to distilled water, and the pH was adjusted to 7; adding 0.05% of cellulase, and performing ultrasonic extraction at 50 ℃ for 2 h; vacuum filtering the extractive solution, vacuum rotary evaporating the filtrate to volume of 10mL, adding 4 times of anhydrous ethanol, mixing, standing in 4 deg.C refrigerator for 12 hr, and centrifuging at 3000r/min for 10min to obtain crude polysaccharide; loading the obtained crude polysaccharide on macroporous adsorbent resin column JAD-1000, eluting with 1 times of column volume of distilled water, eluting with 30% ethanol, and collecting ethanol eluate; recovering the reagent, and vacuum drying to obtain fig polysaccharide.
Example 2:
pulverizing dried fructus fici, sieving with 80 mesh sieve, and collecting coarse powder; mixing the fig dry powder with water and material in a ratio of 45: 1 (mL/g) was added to distilled water, and the pH was adjusted to 8; adding 0.2% cellulase, and performing ultrasonic extraction at 80 deg.C for 3 hr; vacuum filtering the extractive solution, vacuum rotary evaporating the filtrate to volume of 10mL, adding 4 times of anhydrous ethanol, mixing, standing in 4 deg.C refrigerator for 12 hr, and centrifuging at 3000r/min for 10min to obtain crude polysaccharide; loading the obtained crude polysaccharide on macroporous adsorbent resin column, eluting with 2 times of column volume of distilled water, eluting with 50% ethanol, and collecting ethanol eluate; recovering the reagent, and vacuum drying to obtain fig polysaccharide.
Example 3:
pulverizing dried fructus fici, sieving with 80 mesh sieve, and collecting coarse powder; mixing the fig dry powder with water and material in a ratio of 55: 1 (mL/g) was added to distilled water, and the pH was adjusted to 9; adding 0.3% cellulase, and performing ultrasonic extraction at 110 deg.C for 4 hr; vacuum filtering the extractive solution, vacuum rotary evaporating the filtrate to volume of 10mL, adding 4 times of anhydrous ethanol, mixing, standing in 4 deg.C refrigerator for 12 hr, and centrifuging at 3000r/min for 10min to obtain crude polysaccharide; loading the obtained crude polysaccharide on macroporous adsorbent resin column, eluting with 4 times of column volume of distilled water, eluting with 80% ethanol, and collecting ethanol eluate; recovering the reagent, and vacuum drying to obtain fig polysaccharide.
Claims (4)
1. A preparation method for extracting fig polysaccharide from figs is characterized by comprising the following steps:
1) pulverizing dried fructus fici, sieving with 80 mesh sieve, and collecting coarse powder;
2) mixing the fig dry powder with water and material in a ratio of 35: 1-55: 1 (mL/g) is added into distilled water, and the pH is adjusted to 7-9;
3) adding 0.05% -0.3% of cellulase, and performing ultrasonic extraction at the temperature of 50-110 ℃ for 2-4 h;
4) vacuum filtering the extractive solution, vacuum rotary evaporating the filtrate to volume of 10mL, adding 4 times of anhydrous ethanol, mixing, standing in 4 deg.C refrigerator for 12 hr, and centrifuging at 3000r/min for 10min to obtain crude polysaccharide;
5) passing the obtained crude polysaccharide through a macroporous adsorption resin column, eluting by using distilled water with the volume of 1-4 times of the column volume, eluting by using 30-80% ethanol, and collecting ethanol eluent;
6) recovering the reagent, and vacuum drying to obtain fig polysaccharide.
2. The method for extracting fig polysaccharide from fig according to claim 1, wherein the water-to-material ratio in step 2) is 35: 1-55: 1 (mL/g).
3. The method for extracting fig polysaccharide from fig according to claim 1, wherein 0.05% -0.3% of cellulase is added in step 3), the temperature is 50-110 ℃, and the ultrasonic treatment is carried out for 2-4 h.
4. The process of claim 1, wherein said macroporous adsorbent resin in step 5) is one of JAD-1000, ZJX, HD-8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811355555.2A CN111187358A (en) | 2018-11-15 | 2018-11-15 | Preparation method of fig polysaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811355555.2A CN111187358A (en) | 2018-11-15 | 2018-11-15 | Preparation method of fig polysaccharide |
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| Publication Number | Publication Date |
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| CN111187358A true CN111187358A (en) | 2020-05-22 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201811355555.2A Withdrawn CN111187358A (en) | 2018-11-15 | 2018-11-15 | Preparation method of fig polysaccharide |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114042081A (en) * | 2021-12-10 | 2022-02-15 | 江苏省中国科学院植物研究所 | Application of fig polysaccharide |
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2018
- 2018-11-15 CN CN201811355555.2A patent/CN111187358A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114042081A (en) * | 2021-12-10 | 2022-02-15 | 江苏省中国科学院植物研究所 | Application of fig polysaccharide |
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Application publication date: 20200522 |