CN111165810A

CN111165810A - Production method of ferment and fermentation device thereof

Info

Publication number: CN111165810A
Application number: CN202010171767.6A
Authority: CN
Inventors: 孙培雄
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-03-12
Filing date: 2020-03-12
Publication date: 2020-05-19

Abstract

The invention relates to the field of food, in particular to a production method and application of ferment. Taking fresh Xinhui citrus reticulata pulp, juicing, filling into a fermentation tank, sealing, injecting 0.3-0,5% of ozone in the total volume of air into an oxygen-containing space, and enabling aerobic bacteria active on the liquid surface to touch the ozone and be killed so as to achieve the purpose of killing the aerobic bacteria; the following equipment is adopted for assistance; the utility model provides a production of ferment is equipped, contains jar (1), is facing down arranging a elevating gear (5) on the jar body, and elevating gear has arranged down lift axle (4), and the lift axle tip contains the atmoseal seat, and the atmoseal seat breaks away from the jar inner wall relatively, can be driven by the lift axle and go up and down.

Description

Production method of ferment and fermentation device thereof

Technical Field

The invention relates to the field of food, in particular to a method for producing ferment and a fermentation device thereof.

Background

The patent belongs to the deepened design of ZL 201610547923.8;

there are many enzyme products on the market, including imported, japan, taiwan. The Korea and domestic multiple enzyme products are expensive and mixed with different fishes and dragons, which brings confusion and deception to consumers. The main reasons for confusion are: the state does not have a unified standard of the products at present, and most of the products are imported according to the standard of fruit juice beverage of the people's republic of China, so that the contradiction is generated: the fruit and vegetable beverage countries have strict regulations, and particularly have strict limitations on microorganisms, so the products are produced and processed by adopting a high-temperature sterilization treatment process before and after filling, and the high-temperature sterilization can kill all microorganisms and inactivate enzymes (enzymes). Therefore, the general phenomenon of enzyme products on the current market is generated: if the microorganism is not overproof, the enzyme is not active. Then what the components of the ferment are: what process is adopted to achieve that the beneficial components are not damaged is the key point for producing the ferment: as the name suggests, the ferment is a mixture of metabolites produced by biological fermentation of fruits and vegetables and enzymes (acid soluble protein) as main components, and besides, most of the components are amino acids, vitamins, minerals, trace elements, small peptides and polysaccharides, so the fermentation of the ferment is the most difficult to solve: to control the generation of undesired bacteria, the method of controlling undesired bacteria is generally to strictly sterilize the equipment and the substrate, to heat-sterilize the air, and to ferment in an automated manner as much as possible. If the mixed bacteria or the bacteriophage are polluted in the fermentation process, the fermentation process is influenced, the yield of the fermentation product is reduced, the fermentation process is serious, the fermentation process even fails, the fermentation product is required to be poured completely, and the ferment fermentation can not be carried out, taking the Xinhui mandarin orange as an example: the harvest season of Xinhui oranges is from 10 to 12 months of the year, and no meat or peel is needed by local growers or purchasers, so that the unnecessary oranges and flesh can be disposed conveniently and become polluted: 1, the peeling damages the coating of the pulp, and the juice flows out to cause pollution. 2, the container is unclean or used repeatedly and causes pollution, 3, time, pulp after peeling is piled up and is extruded and have a lot of fruit juice outflow, and oxidative corruption, 4, the improper pollution that leads to of juicing process control. 5, pollution and the like are caused because the can is not sterilized in time after being filled. More importantly, the fruits and vegetables cannot be sterilized at high temperature during fermentation because the high temperature can cause the inactivation of the original enzymes of the fruits and vegetables.

Researches show that citrus pulp collected in a citrus orchard or a square has pollution with different degrees, the total number of the mold is more than 1.76cfu/ml multiplied by 106 through the detection of total number of microbial colonies and the mold is positive, tests show that the mold and the mold are aerobic bacteria, namely the mold and the mold can effectively kill the aerobic bacteria in liquid to remove most harmful bacteria, namely the citrus pulp needs to produce enzyme, the juice is squeezed and canned, and then the treatment for killing the aerobic bacteria is carried out, the traditional sterilization method is to sterilize at high temperature, so that the enzyme in fruit juice is destroyed, the fermentation period after inoculation is long, the total amount of the enzyme is low, and the total amount of the enzyme is few. Finding a normal-temperature sterilization method which can effectively remove aerobic bacteria and reserve anaerobic bacteria under the condition of not damaging the existing enzymes is one of the primary conditions for producing the citrus reticulata enzyme; the second condition is as follows: culturing a probiotic flora with high enzyme yield and stronger vitality in the environment with the pH value of 2.6-3.3 to improve the quality of the enzyme; and under the third condition, when the total enzyme value reaches the maximum value, the method can sterilize at normal temperature in time to stop continuous fermentation without damaging the activity of the enzyme, and a safe and reliable enzyme product can be produced by adopting the three conditions. However, no suitable technical means can simultaneously achieve the above purposes.

In the current fermentation, the following problems exist:

1. the method has high capacity requirement, but the capacity can not always meet the requirement;

2. for oxygen control fermentation and anaerobic fermentation, the sealing performance is poor or the tank is frequently opened, so that fermentation failure is easily caused;

3. the fermentation tank is large, the fermentation conditions at different positions can be different, and therefore, sampling and adding and parameter acquisition are difficult in the industry.

Disclosure of Invention

The purpose of the invention is as follows: in order to provide a method for producing ferments with better effect and a fermentation apparatus thereof, the following practical technical effects are considered.

In order to achieve the purpose, the invention adopts the following technical scheme:

a method for producing ferment is characterized by comprising the following steps,

taking fresh Xinhui citrus reticulata pulp, juicing, filling into a fermentation tank, sealing, injecting 0.3-0,5% of ozone in the total volume of air into an oxygen-containing space, and enabling aerobic bacteria active on the liquid surface to touch the ozone and be killed so as to achieve the purpose of killing the aerobic bacteria;

standing for 24 months, and stopping fermentation when the pH value is 2.4-3.1; at the moment, the flora at least comprises one or more of lactobacillus casei, lactobacillus paracasei, lactobacillus brevis and bacillus aceticus, and the flora is separated out to be used as a zymocyte for standby;

when the fresh Xinhui citrus pulp is juiced, filling a pot; the following equipment is adopted for assistance; a ferment production device comprises a pot 1, wherein a lifting device 5 is arranged on a pot body in an upward-downward manner, a lifting shaft 4 is arranged on the lifting device in the downward direction, the end part of the lifting shaft comprises an air seal seat, the air seal seat comprises two plates, the two plates are an upper plate 19 and a lower plate 20 respectively, an edge side extrusion part 15 is arranged between the upper plate 19 and the lower plate 20 in a heat seal manner, and the edge side extrusion part 15 is made of a circle of rubber material; the middle part of the side extrusion part 15 is provided with an inner inflation space 16, the inner inflation space 16 can be inflated by an inflation fixing tube 18, and the side extrusion part 15 on the side expands when the inflation fixing tube 18 faces the inner inflation space 16, so that the inner wall of the tank can be extruded; the inflation fixing tube 18 is pumped outwards, and the side extrusion part 15 at the side is retracted, so that the air seal seat is relatively separated from the inner wall of the pot and can be driven by the lifting shaft to lift;

the two air seal seats are arranged on the air seal seat, so that a cooperative closed system can be formed;

the upper part of the closed system is not treated, the lower part is a fermentation space, and the loading is 96 percent of the fermentation volume;

the gas seal device also comprises a gas inlet pipe 6 penetrating through the gas seal seat, and gas or substances can be added below the gas seal seat through the gas inlet pipe 6; the inlet pipe 6 is hermetically connected with the air seal seat, and sealing is realized through a sealing part;

the sealing part structure is: the parts of the inlet pipe penetrating through the upper plate 19 and the lower plate 20 respectively comprise outer clamping parts 21, and the outer clamping parts 21 are embedded in the hole wall grooves of the upper plate 19 and the lower plate 20; the upper and lower parts of the outer clamping part 21 respectively comprise a sealing ring;

the inlet pipe 6 is connected with an ozone source or a material pump;

the method also comprises a step of adjusting sugar degree before being filled into a fermentation tank, wherein the step of adjusting sugar degree is to add 7-5% of sucrose when the sugar degree of the citrus reticulata juice is 6-9% to prepare a material with the total sugar degree of 11-13%; the material is added through the inlet pipe 6; the multifunctional pipeline 9 penetrates through the air sealing seat, and the end part of the multifunctional pipeline 9 comprises a multifunctional box; the multifunctional pipeline 9 can lift along with the lifting of the air seal seat; the multifunctional pipeline 9 is a sensor circuit, a sensor is arranged in a multifunctional box below the multifunctional pipeline, and the sensor is any one of a PH sensor, a temperature sensor and a sugar degree detection sensor; the parameters of multiple layers can be obtained along with the lifting of the air seal seat.

The invention further has the technical scheme that the filling amount of the liquid to be sterilized is controlled to be 96 percent of the total volume of the tank body, aerobic bacteria are floated on the liquid surface and the middle upper part to be beneficial to centralized elimination, any one of the following modes is adopted for sterilization, A, alcohol is used for liquid surface sterilization, and the aerobic bacteria can be eliminated by spraying or covering the alcohol on the liquid surface; b, sterilizing by using an ozone space, injecting 0.3-0,5% of ozone in the total volume of the air into the oxygen-containing space, and killing aerobic bacteria which are activated on the liquid surface by touching the ozone to kill the aerobic bacteria; separating the strains and further comprising a step of expanding culture, wherein the expanding culture is to take fresh citrus reticulata pulp, squeeze juice and put the juice into a fermentation tank, the filling amount is 96 percent of the total volume, and a 4 percent space is reserved;

adding 7-5% of sucrose when sugar degree of Xinhui citrus juice is 6-9%, and preparing into material with total sugar degree of 11-13%;

inputting ozone which accounts for 0.3 percent of the upper space of the feed liquid level, and killing aerobic flora; removing mixed bacteria;

then inoculating the separated flora according to 1-10% of the total amount of the fermentation liquor, and sealing;

standing at normal temperature for 3 months;

stopping fermentation when the pH value is 2.4-3.1, and stopping fermentation in time;

the multifunctional pipeline 9 is a sampling line, the end part of the multifunctional pipeline 9 comprises a sampling port, and the multifunctional pipeline 9 is connected with a pump and a sampling container; the air seal seat can be lifted along with the lifting of the air seal seat, so that a plurality of layers of samples can be taken.

The further technical scheme of the invention is that the fermentation prevention means that the cold bactericide dimethyl dicarbonate is input into the tank body, so that the effects of keeping alive and stopping fermentation can be achieved; the input concentration is 0.1 percent of the total liquid mass in the tank body; the method also comprises the step of inducing the fermentation strain, wherein the induction is to obtain a high-yield acid-soluble protease strain group after compound mutagenesis by adopting ultraviolet rays and nitrosoguanidine; the fresh Xinhui citrus pulp treatment also comprises the steps of removing impurities and decay, removing pesticide residues, leaching, dehydrating, juicing and filtering; the method also comprises the step of inoculating a fermentation strain into any one or more of Xinhui citrus juice, mulberry juice, apple juice, lemon juice, dragon fruit juice, Chinese yam juice, watermelon juice and tomatoes for fermentation for 3 months, and obtaining the natural ferment containing acid soluble protein, amino acid and small peptide after the fermentation is finished.

A processing method for preventing materials from being too few to ferment is characterized in that enzyme production equipment comprises a tank 1, a lifting device 5 is arranged on a tank body in a downward mode, a lifting shaft 4 is arranged on the lifting device in a downward mode, the end portion of the lifting shaft comprises an air seal seat, the air seal seat comprises two plates, the two plates are an upper plate 19 and a lower plate 20 respectively, an edge side extrusion portion 15 is arranged between the upper plate 19 and the lower plate 20 in a heat sealing mode, and the edge side extrusion portion 15 is made of a circle of rubber materials; the middle part of the side extrusion part 15 is provided with an inner inflation space 16, the inner inflation space 16 can be inflated by an inflation fixing tube 18, and the side extrusion part 15 on the side expands when the inflation fixing tube 18 faces the inner inflation space 16, so that the inner wall of the tank can be extruded; the gas-filled fixing tube 18 is evacuated outward and the side pressing portion 15 of the side is retracted, so that the gas seal is relatively separated from the inner wall of the can and can be lifted by the lifting shaft.

the inlet pipe 6 is connected with an ozone source or a material pump;

the capacity adjustment is realized by adjusting the height of the air seal seat.

A method for avoiding external influence sampling without opening a tank is characterized in that enzyme production equipment comprises a tank 1, a lifting device 5 is arranged on a tank body in an upward-downward mode, a lifting shaft 4 is arranged on the lifting device in a downward mode, the end portion of the lifting shaft comprises an air seal seat, the air seal seat comprises two plates, the two plates are an upper plate 19 and a lower plate 20 respectively, an edge side extrusion portion 15 is arranged between the upper plate 19 and the lower plate 20 in a heat sealing mode, and the edge side extrusion portion 15 is made of a circle of rubber material; the middle part of the side extrusion part 15 is provided with an inner inflation space 16, the inner inflation space 16 can be inflated by an inflation fixing tube 18, and the side extrusion part 15 on the side expands when the inflation fixing tube 18 faces the inner inflation space 16, so that the inner wall of the tank can be extruded; the gas-filled fixing tube 18 is evacuated outward and the side pressing portion 15 of the side is retracted, so that the gas seal is relatively separated from the inner wall of the can and can be lifted by the lifting shaft.

the inlet pipe 6 is connected with an ozone source or a material pump;

the multifunctional pipeline 9 penetrates through the air sealing seat, and the end part of the multifunctional pipeline 9 comprises a multifunctional box; the multifunctional pipeline 9 can lift along with the lifting of the air seal seat; the multifunctional pipeline 9 is a sampling line, the end part of the multifunctional pipeline 9 comprises a sampling port, and the multifunctional pipeline 9 is connected with a pump and a sampling container; the air seal seat can be lifted along with the lifting of the air seal seat, so that a plurality of layers of samples can be taken.

A method for acquiring parameters without opening a pot and avoiding external influence is characterized in that enzyme production equipment comprises a pot 1, wherein a lifting device 5 is arranged on a pot body in an upward-downward mode, a lifting shaft 4 is arranged on the lifting device in a downward mode, the end portion of the lifting shaft comprises an air seal seat, the air seal seat comprises two plates, the two plates are an upper plate 19 and a lower plate 20 respectively, an edge side extrusion portion 15 is arranged between the upper plate 19 and the lower plate 20 in a heat sealing mode, and the edge side extrusion portion 15 is made of a circle of rubber material; the middle part of the side extrusion part 15 is provided with an inner inflation space 16, the inner inflation space 16 can be inflated by an inflation fixing tube 18, and the side extrusion part 15 on the side expands when the inflation fixing tube 18 faces the inner inflation space 16, so that the inner wall of the tank can be extruded; the gas-filled fixing tube 18 is evacuated outward and the side pressing portion 15 of the side is retracted, so that the gas seal is relatively separated from the inner wall of the can and can be lifted by the lifting shaft.

the sealing part structure is: the parts of the inlet pipe penetrating through the upper plate 19 and the lower plate 20 respectively comprise outer clamping parts 21, and the outer clamping parts 21 are embedded in the hole wall grooves of the upper plate 19 and the lower plate 20; the upper and lower parts of the outer clamping part 21 respectively comprise a sealing ring; the inlet pipe 6 is connected with an ozone source or a material pump;

the multifunctional pipeline 9 penetrates through the air sealing seat, and the end part of the multifunctional pipeline 9 comprises a multifunctional box; the multifunctional pipeline 9 can lift along with the lifting of the air seal seat; the multifunctional pipeline 9 is a sensor circuit, a sensor is arranged in a multifunctional box below the multifunctional pipeline, and the sensor is any one of a PH sensor, a temperature sensor and a sugar degree detection sensor; the parameters of multiple layers can be obtained along with the lifting of the air seal seat.

Compared with the prior art, the invention adopting the technical scheme has the following beneficial effects: sterilizing at normal temperature without inactivation, fully utilizing the Xinhui citrus pulp, and having high fermentation success rate; the problems in the prior art are solved, and the original technical problems are solved by the same means creatively; the time of re-fermentation after inoculation can be achieved only by 90 days, and the effect of conventional fermentation for 720 days can be achieved; the conventional method is high-temperature sterilization, all bacteria are killed at the same time of high-temperature sterilization, original natural enzymes are killed, the investment is relatively large, and the large-scale production is not facilitated; the sugar depends on the fermentation time and the fermentation period of the microorganisms, too little sugar, incomplete fermentation of the substrate and too much sugar increase the fermentation time, and the sugar is prepared just right; the oxygen-containing space is less than 5 percent of the total volume, and can be well regulated by anaerobic control;

meanwhile, in order to match the process, no matter how many fermentation raw materials are, the process can be realized, and special mechanical devices and equipment are matched; the device is more pioneering and has the following characteristics: 1. grading the volume; 2. isolating; 3. the fermentation of the material can be realized; 4. opening-free multi-layer accurate sampling; 5. and (4) acquiring multilayer accurate parameters without opening the tank.

Drawings

To further illustrate the present invention, further description is provided below with reference to the accompanying drawings:

FIG. 1 is a schematic structural view of a two-layer embodiment;

FIG. 2 is a schematic structural view of a single layer embodiment;

FIG. 3 is a schematic view of a multi-function embodiment;

FIG. 4 is a schematic view of the gas seal seat;

FIG. 5 is a schematic view of a wall penetrating portion of a pipe;

FIG. 6 is a partial piping schematic of FIG. 5;

FIG. 7 is a process diagram of the invention;

wherein: 1. a tank; 2. a lower layer closing plate; 3. an upper layer closing plate; 4. a lifting shaft; 5. a lifting device; 6. feeding a pipe; 7. a sealing portion; 8. a single-layer closure panel; 9. a multifunctional pipeline; 10. a multifunctional box; 11. closing the edges; 12. a height measuring device; 13. a cylinder block; 14. ozone is supplied to the structure; 15. a side pressing portion; 16. an interior plenum space; 17. a hollow portion; 18. an inflatable fixed tube; 19. an upper plate; 20. a lower layer plate; 21. an outer clamping portion; 22. a first sealing ring; 23. and a second sealing ring.

Detailed Description

The present invention will be further illustrated with reference to the accompanying drawings and specific embodiments, which are to be understood as merely illustrative of the invention and not as limiting the scope of the invention. In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", "top", "bottom", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, unless expressly stated or limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, as they may be fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

The patent provides a plurality of parallel schemes, and different expressions belong to an improved scheme based on a basic scheme or a parallel scheme. Each solution has its own unique features.

the inlet pipe 6 is connected with an ozone source or a material pump;

the method also comprises a step of adjusting sugar degree before being filled into a fermentation tank, wherein the step of adjusting sugar degree is to add 7-5% of sucrose when the sugar degree of the citrus reticulata juice is 6-9% to prepare a material with the total sugar degree of 11-13%; the material is added through the inlet pipe 6; the multifunctional pipeline 9 penetrates through the air sealing seat, and the end part of the multifunctional pipeline 9 comprises a multifunctional box; the multifunctional pipeline 9 can lift along with the lifting of the air seal seat; the multifunctional pipeline 9 is a sensor circuit, a sensor is arranged in a multifunctional box below the multifunctional pipeline, and the sensor is any one of a PH sensor, a temperature sensor and a sugar degree detection sensor; the parameters of multiple layers can be obtained along with the lifting of the air seal seat: the technical scheme of the invention has the following substantial technical effects and the realization process thereof: compared with the fermentation tank in the prior art, the capacity grading device has no capacity regulation function at all, cannot flexibly adapt to the amount of supplied materials, and cannot install and purchase a new fermentation tank at an expensive price; 2. isolation, in addition to traditional airtight isolation, the present patent can also adopt multilayer isolation or one-layer isolation, as shown in fig. 1-3, which shows double-layer isolation, single-layer isolation, etc., and the top of the tank is not too complicated; 3. the fermentation of the material can be realized; 4. opening-free multi-layer accurate sampling; 5. and (4) acquiring multilayer accurate parameters without opening the tank.

It should be noted that the mechanical device of this patent is patentable alone.

It is expressed as follows:

the enzyme production equipment is characterized by comprising a pot 1, wherein a lifting device 5 is arranged on a pot body in an upward-downward manner, a lifting shaft 4 is arranged on the lifting device in a downward manner, the end part of the lifting shaft comprises an air seal seat, the air seal seat comprises two plates, the two plates are an upper plate 19 and a lower plate 20 respectively, an edge side extrusion part 15 is thermally sealed between the upper plate 19 and the lower plate 20, and the edge side extrusion part 15 is made of a circle of rubber material; the middle part of the side extrusion part 15 is provided with an inner inflation space 16, the inner inflation space 16 can be inflated by an inflation fixing tube 18, and the side extrusion part 15 on the side expands when the inflation fixing tube 18 faces the inner inflation space 16, so that the inner wall of the tank can be extruded; the gas-filled fixing tube 18 is evacuated outward and the side pressing portion 15 of the side is retracted, so that the gas seal is relatively separated from the inner wall of the can and can be lifted by the lifting shaft.

the inlet pipe 6 is connected with an ozone source or a material pump;

Researches find that when the content of the ferment fermentation tank is controlled to be 96% of the total volume of the tank body, a large amount of aerobic floras can emerge on the liquid surface, which is beneficial to centralized elimination, the oxygen-containing space with the volume less than 5% keeps a relatively anaerobic space after eliminating the aerobic floras, when the oxygen-containing space is more than 5%, the oxygen content in the space can maintain the survival conditions of the aerobic floras, which is not beneficial to the safe production of products, and when the oxygen content is less than 4%, the oxygen content in the space is less than the growth of the good itching floras, which is beneficial to the reproduction of anaerobic. And B, comparing with the prior art that no matter a grower or a purchaser needs skin or meat, the unwanted citrus pulp can be disposed conveniently and polluted, and the prior art can greatly utilize the citrus pulp and extract beneficial enzymes from the citrus pulp.

As a further preference, the fermentation tank is further provided with a sugar degree adjusting step before being filled, wherein the sugar degree adjusting step is to add 7-5% of sucrose when the sugar degree of the citrus reticulata juice is measured to be 6-9% to prepare a material with the total sugar degree of 11-13%.

This patent especially provides the system fermentation process and the control condition of new meeting citrus ferment, the composition and the application method of fermentation product, provides a safe effectual ferment product for vast consumer. The mechanism of the invention is to utilize primary strains, and the experiment shows that: all fruits and vegetables have different numbers of bacterial strains, and the fresh Xinhui orange juice contains: the control method for large-scale fermentation of the fruit and vegetable juice provides a manufacturing approach which is easy to operate and is suitable for large-scale deep processing of agricultural products.

The invention aims to find a safe and effective normal-temperature sterilization method in numerous tests, and the Xinhui citrus ferment stock solution prepared by the method has no harmful bacteria detected.

The process flow comprises the following steps: removing impurities and corrosion of fruits and vegetables, removing pesticide residues, leaching, dehydrating, juicing, filtering, canning, degassing, sterilizing, adding sugar, inoculating, fermenting, controlling the process, testing ph, stopping fermentation, and grading, sieving and sterilizing. Various desired products can be made according to existing processes such as: tablets, capsules, granules. Enzyme stock solution, beverage, etc.

More specifically: taking fresh Xinhui citrus pulp, juicing, filling into a fermentation tank, wherein the filling amount is 96 percent of the total volume, adding sugar, measuring the sugar degree of the Xinhui citrus juice to be 6-9 percent, adding 7-5 percent of cane sugar, preparing into the juice with the total sugar degree of 11-13 percent, sealing, inputting 0.3 percent of ozone, killing aerobic flora (calculated by the space above the material liquid level), standing for fermentation for 24 months, and measuring the total microbial count and the coliform detection in an enzyme solution with the pH value of 2.6-3.3: the detection result is the total number of microbial colonies: 1.76cfu/ml X106, coliform group is negative. The microbial genera in the fermentation liquor are identified by a bacteria 16srdna molecular identification method, and the identification result shows that the product contains lactobacillus casei, lactobacillus paracasei and lactobacillus brevis. And (5) no mixed bacteria. The pH value point for terminating the fermentation is 2.4-3.1; the contents of the product are measured at this point: acid soluble protein: 44.6%, total amount of 17 amino acids: 41.2 percent and 31 percent of small peptide; (after the report), this bacterial group was isolated and cultured in the same manner, and used as a fermentation bacterial strain 10 days before inoculation and activation.

Removing aerobic bacteria: the research finds that: when the fruit juice is canned, aerobic bacteria strive for the liquid surface. In order to obtain survival, there are two methods to kill aerobic bacteria. Firstly, the liquid surface is sterilized by using alcohol, and the aerobic bacteria can be eliminated by spraying the alcohol or covering the liquid surface with the alcohol. But the amount is difficult to control, the effect is not obvious, anaerobic bacteria are damaged more, and the fermentation is slow. Secondly, ozone space sterilization is used. When 0.3-0,5% ozone is injected into the oxygen-containing space, the aerobic bacteria which are active on the liquid surface can touch the ozone and be killed, thereby achieving the purpose of killing the aerobic bacteria,

the control of the acidity to the pH value of 2.6-3.3 is an important condition for the safe storage of the fermentation product, general microorganisms cannot survive in the secondary environment, and meanwhile, the lactic acid has the function of helping human digestion, helping intestinal health and keeping ecological balance. The total amount of enzyme is the maximum, so the fermentation is stopped at the best time;

when the fermentation is finished, the fermentation can be stopped while the normal temperature sterilization is effectively carried out to keep the activity of the enzyme, so that the consistency of the product is kept.

The invention principle is as follows: in the large-scale production, the sterilization in a fermentation tank without a stirring system (the volume is 10 cubic meters) is almost impossible, the patent initiatively inspires me from an oxygenator in a super-large fish tank, and the cold bactericide is prepared by using the principle of oxygenating the fish tank: the dimethyl carbonate is input into the tank body, thus achieving the aim. Verification found that "dimethyl carbonate" was input. When the concentration is 0.1 percent/cubic meter, sampling is carried out for 24 hours, and the microbial examination meets the requirements of national food standards.

Preferably, the strain isolation step further comprises an expanded culture step, wherein the expanded culture step is to take fresh citrus reticulata pulp, squeeze the juice, and place the juice in a fermentation tank, wherein the filling amount is 96% of the total volume, and a 4% space is reserved;

standing at normal temperature for 3 months;

when the pH value is 2.4-3.1, the fermentation is stopped, and the fermentation is stopped in time.

As a further optimization, the fermentation prevention means that the cold bactericide dimethyl dicarbonate is input into the tank body, so that the effects of keeping alive and stopping fermentation can be achieved; the input concentration is 0.1% of the total liquid mass in the tank.

The method provides the effects of enlarging culture and further fermenting.

The invention further adopts the technical scheme that the method also comprises the step of inducing the zymocyte, wherein the induction is to obtain the acid soluble protein (enzyme) high-yield strain group after the compound mutagenesis of ultraviolet rays and nitrosoguanidine is adopted. The bacterial colony which can still have stronger activity in higher acidity is obtained by 720 days of normal temperature anaerobic fermentation of Xinhui mandarin orange pure juice, and the bacterial colony is mainly identified by a bacterium 16SrDNA molecule: the strain group is a flora such as lactobacillus casei, lactobacillus paracasei, lactobacillus brevis, bacillus aceticus and the like, after ultraviolet rays and nitrosoguanidine compound mutagenesis are adopted, a high-yield strain group of acid soluble protein (enzyme) is obtained, 1-5% of the strain group is inoculated in Xinhui orange juice for fermentation for 3 months, and Huazhong agriculture detection shows that: total amount of acid soluble protein (ferment): 44.62% assay selected the measured total activity 1519u/g of the partial enzyme. The strain has stronger activity and higher enzyme production capability in the environment with the pH value of 2.6-3.5, and has stable performance in industrial production. The enzyme production activity of the original citrus juice flora is more new than that of the original citrus juice flora: 11.4574u/g is increased by 1508 u/g.

The fresh Xinhui citrus pulp treatment also comprises the steps of removing impurities and decay, removing pesticide residues, rinsing, dehydrating, juicing and filtering.

The method further comprises the step of inoculating the fermentation strain to one or more of citrus reticulata juice, mulberry juice, apple juice, lemon juice, dragon fruit juice, Chinese yam juice, watermelon juice and tomato juice, and fermenting for 3 months to obtain the natural ferment containing acid soluble protein, amino acid and small peptide.

Standing for 24 months, terminating fermentation, grading, sieving, sterilizing, and terminating fermentation. The 24 months is about two years, and the total days may slightly fluctuate.

The invention removes impurities and decay of fruits and vegetables, removes pesticide residues, leaches, dehydrates, squeezes and filters, cans, degasses, sterilizes, matches sugar, inoculates, ferments, controls the process, tests ph, stops fermenting, sieves and sterilizes in grades. Various desired products can be made according to existing processes such as: tablets, capsules, granules. Enzyme stock solution, beverage, etc. A safe and effective normal-temperature sterilization method is found in numerous tests, and no harmful bacteria are detected in the Xinhui citrus ferment stock solution prepared by the method.

The acidity control is an important condition for safe storage of the fermentation product, microorganisms cannot survive in the secondary environment generally, and the lactic acid has the function of helping human digestion to help intestinal health and keep ecological balance. The total amount of enzyme is the maximum, so the fermentation is stopped at the best time;

The time of re-fermentation after inoculation can be achieved only by 90 days, and the effect of conventional fermentation for 720 days can be achieved;

the conventional method is high-temperature sterilization, all bacteria are killed at the same time of high-temperature sterilization, original natural enzymes are killed, the investment is relatively large, and the large-scale production is not facilitated;

the sugar depends on the fermentation time and the fermentation period of the microorganisms, too little sugar, incomplete fermentation of the substrate and too much sugar increase the fermentation time, and the sugar is prepared just right;

the oxygen-containing space is less than 5 percent of the total volume, and can be well regulated by anaerobic control;

the conventional fermentation termination needs to add inhibitor or high-temperature sterilization treatment before preparation and canning, but the invention can terminate the fermentation at any time and any place.

Scheme II:

a method for killing aerobic bacteria at normal temperature is characterized in that the loading of liquid to be sterilized is controlled to be 96% of the total volume of a tank body, aerobic bacteria groups float on the liquid surface and the middle upper part to be beneficial to centralized killing, and the liquid is sterilized by adopting any one of the following modes, wherein A, alcohol is used for liquid surface sterilization, and the aerobic bacteria can be killed by spraying or covering the alcohol on the liquid surface; b, ozone space sterilization is adopted, 0.3-0,5% of ozone of the total volume of air is injected into the oxygen-containing space, and aerobic bacteria which are active on the liquid surface can touch the ozone to be killed, so that the aim of killing the aerobic bacteria is fulfilled.

The third scheme is as follows:

use of Xinhui citrus in preparing ferment is provided.

Take Xinhui citrus ferment as an example:

culturing probiotic flora with high enzyme yield in the fermented product, wherein the pH value of the probiotic flora is 2.6-3.3

Creatively, the above effects exist independently, and the combination of the above results can be completed by a set of structure. Some details are not shown in the figures.

It should be noted that the plurality of schemes provided in this patent include their own basic schemes, which are independent of each other and are not restricted to each other, but they may be combined with each other without conflict, so as to achieve a plurality of effects.

The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are intended to illustrate the principles of the invention, but that various changes and modifications may be made without departing from the spirit and scope of the invention, and the invention is to be limited to the embodiments described above.

Claims

1. A method for producing ferment is characterized by comprising the following steps of taking fresh citrus reticulata pulp, juicing, putting the juice into a fermentation tank, sealing the fermentation tank, injecting ozone which accounts for 0.3-0.5% of the total volume of air into an oxygen-containing space, and killing aerobic bacteria which are active on the liquid surface by touching the ozone so as to kill the aerobic bacteria;

when the fresh Xinhui citrus pulp is juiced, filling a pot; the following equipment is adopted for assistance; a production device of ferment comprises a pot (1), wherein a lifting device (5) is arranged on a pot body downwards, a lifting shaft (4) is arranged on the lifting device downwards, the end part of the lifting shaft comprises an air seal seat, the air seal seat comprises two plates which are an upper plate (19) and a lower plate (20) respectively, a side extruding part (15) is thermally sealed between the upper plate (19) and the lower plate (20), and the side extruding part (15) is made of a circle of rubber material; the middle part of the side extrusion part (15) is provided with an internal inflation space (16), the internal inflation space (16) can be inflated by an inflation fixing tube (18), and the side extrusion part (15) on the side expands when the inflation fixing tube (18) faces the internal inflation space (16) to extrude the inner wall of the pot; the inflation fixing tube (18) is pumped outwards, and the side extrusion part (15) at the side is retracted, so that the air seal seat is relatively separated from the inner wall of the pot and can be driven by the lifting shaft to lift;

the gas seal device also comprises a gas inlet pipe (6) penetrating through the gas seal seat, and gas or substances can be added below the gas seal seat through the gas inlet pipe (6); the inlet pipe (6) is hermetically connected with the air seal seat, and the sealing is realized through a sealing part;

the sealing part structure is: the parts of the inlet pipe penetrating through the upper plate (19) and the lower plate (20) respectively comprise outer clamping parts (21), and the outer clamping parts (21) are embedded in the hole wall grooves of the upper plate (19) and the lower plate (20); the upper part and the lower part of the outer clamping part (21) respectively comprise a sealing ring;

the inlet pipe (6) is connected with an ozone source or a material pump;

the method also comprises a step of adjusting sugar degree before being filled into a fermentation tank, wherein the step of adjusting sugar degree is to add 7-5% of sucrose when the sugar degree of the citrus reticulata juice is 6-9% to prepare a material with the total sugar degree of 11-13%; the material is added through a feeding pipe (6); the multifunctional pipeline (9) penetrates through the air seal seat, and the end part of the multifunctional pipeline (9) comprises a multifunctional box; the multifunctional pipeline (9) can be lifted along with the lifting of the air seal seat; the multifunctional pipeline (9) is a sensor circuit, a sensor is arranged in a multifunctional box below the multifunctional pipeline, and the sensor is any one of a PH sensor, a temperature sensor and a sugar degree detection sensor; the parameters of multiple layers can be obtained along with the lifting of the air seal seat.

2. The method for producing ferment as claimed in claim 1, wherein the amount of liquid to be sterilized is controlled to 96% of the total volume of the tank, aerobic bacteria are present on the liquid surface and the middle upper part to be sterilized, and the aerobic bacteria can be sterilized by any one of the following methods, wherein A, the liquid surface of the alcohol is sterilized, and the aerobic bacteria can be sterilized by spraying or covering the liquid surface with the alcohol; b, sterilizing by using an ozone space, injecting 0.3-0,5% of ozone in the total volume of the air into the oxygen-containing space, and killing aerobic bacteria which are activated on the liquid surface by touching the ozone to kill the aerobic bacteria; separating the strains and further comprising a step of expanding culture, wherein the expanding culture is to take fresh citrus reticulata pulp, squeeze juice and put the juice into a fermentation tank, the filling amount is 96 percent of the total volume, and a 4 percent space is reserved;

then inoculating the separated flora according to 1-10% of the total amount of the fermentation liquor, and sealing; standing at normal temperature for 3 months;

stopping fermentation when the pH value is 2.4-3.1, and stopping fermentation in time; the multifunctional pipeline (9) is a sampling circuit, the end part of the multifunctional pipeline (9) comprises a sampling port, and the multifunctional pipeline (9) is connected with a pump and a sampling container;

the air seal seat can be lifted along with the lifting of the air seal seat, so that a plurality of layers of samples can be taken.

3. The method for producing ferment according to claim 1, wherein the fermentation inhibition means that the cold bactericide dimethyldicarbonate is fed into the tank to achieve the effects of keeping the ferment alive and stopping the fermentation; the input concentration is 0.1 percent of the total liquid mass in the tank body; the method also comprises the step of inducing the fermentation strain, wherein the induction is to obtain a high-yield acid soluble protein (enzyme) strain group after compound mutagenesis by adopting ultraviolet rays and nitrosoguanidine; the fresh Xinhui citrus pulp treatment also comprises the steps of removing impurities and decay, removing pesticide residues, leaching, dehydrating, juicing and filtering; the method also comprises the step of inoculating a fermentation strain into any one or more of Xinhui citrus juice, mulberry juice, apple juice, lemon juice, dragon fruit juice, Chinese yam juice, watermelon juice and tomatoes for fermentation for 3 months, and obtaining the natural ferment containing acid soluble protein, amino acid and small peptide after the fermentation is finished.

4. A treatment method for preventing materials from being too few to ferment is characterized in that enzyme production equipment comprises a tank (1), wherein a lifting device (5) is arranged on a tank body in an upward-downward mode, a lifting shaft (4) is arranged on the lifting device in a downward mode, the end portion of the lifting shaft comprises an air seal seat, the air seal seat comprises two plates which are an upper plate (19) and a lower plate (20) respectively, a side extruding portion (15) is arranged between the upper plate (19) and the lower plate (20) in a heat sealing mode, and the side extruding portion (15) is made of a circle of rubber materials; the middle part of the side extrusion part (15) is provided with an internal inflation space (16), the internal inflation space (16) can be inflated by an inflation fixing tube (18), and the side extrusion part (15) on the side expands when the inflation fixing tube (18) faces the internal inflation space (16) to extrude the inner wall of the pot; the inflation fixing tube (18) is pumped outwards, and the side extrusion part (15) at the side is retracted, so that the air seal seat is relatively separated from the inner wall of the pot and can be driven by the lifting shaft to lift;

the inlet pipe (6) is connected with an ozone source or a material pump;

5. A method for acquiring parameters without opening a tank and avoiding external influence is characterized in that enzyme production equipment comprises a tank (1), wherein a lifting device (5) is arranged on a tank body in an upward-downward mode, a lifting shaft (4) is arranged on the lifting device in a downward mode, the end portion of the lifting shaft comprises an air seal seat, the air seal seat comprises two plates which are an upper plate (19) and a lower plate (20) respectively, a side extruding part (15) is arranged between the upper plate (19) and the lower plate (20) in a heat sealing mode, and the side extruding part (15) is made of a circle of rubber material; the middle part of the side extrusion part (15) is provided with an internal inflation space (16), the internal inflation space (16) can be inflated by an inflation fixing tube (18), and the side extrusion part (15) on the side expands when the inflation fixing tube (18) faces the internal inflation space (16) to extrude the inner wall of the pot; the inflation fixing tube (18) is pumped outwards, and the side extrusion part (15) at the side is retracted, so that the air seal seat is relatively separated from the inner wall of the pot and can be driven by the lifting shaft to lift;

the inlet pipe (6) is connected with an ozone source or a material pump;

the multifunctional pipeline (9) penetrates through the air seal seat, and the end part of the multifunctional pipeline (9) comprises a multifunctional box; the multifunctional pipeline (9) can be lifted along with the lifting of the air seal seat; the multifunctional pipeline (9) is a sampling circuit, the end part of the multifunctional pipeline (9) comprises a sampling port, and the multifunctional pipeline (9) is connected with a pump and a sampling container; the air seal seat can be lifted along with the lifting of the air seal seat, so that a plurality of layers of samples can be taken.

6. The method for sampling the enzyme without opening the tank and avoiding external influence is characterized in that enzyme production equipment comprises the tank (1), a lifting device (5) is arranged on a tank body in an upward-downward mode, a lifting shaft (4) is arranged on the lifting device in a downward mode, the end portion of the lifting shaft comprises an air seal seat, the air seal seat comprises two plates which are an upper plate (19) and a lower plate (20) respectively, a side extruding portion (15) is arranged between the upper plate (19) and the lower plate (20) in a heat sealing mode, and the side extruding portion (15) is made of a circle of rubber material; the middle part of the side extrusion part (15) is provided with an internal inflation space (16), the internal inflation space (16) can be inflated by an inflation fixing tube (18), and the side extrusion part (15) on the side expands when the inflation fixing tube (18) faces the internal inflation space (16) to extrude the inner wall of the pot; the inflation fixing tube (18) is pumped outwards, and the side extrusion part (15) at the side is retracted, so that the air seal seat is relatively separated from the inner wall of the pot and can be driven by the lifting shaft to lift;

the inlet pipe (6) is connected with an ozone source or a material pump;

the multifunctional pipeline (9) penetrates through the air seal seat, and the end part of the multifunctional pipeline (9) comprises a multifunctional box; the multifunctional pipeline (9) can be lifted along with the lifting of the air seal seat; the multifunctional pipeline (9) is a sensor circuit, a sensor is arranged in a multifunctional box below the multifunctional pipeline, and the sensor is any one of a PH sensor, a temperature sensor and a sugar degree detection sensor; the parameters of multiple layers can be obtained along with the lifting of the air seal seat.