CN111155975B - Method for improving yield of biological coal bed gas through feed supplement fermentation - Google Patents
Method for improving yield of biological coal bed gas through feed supplement fermentation Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 29
- 238000000855 fermentation Methods 0.000 title claims abstract description 17
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- 239000006052 feed supplement Substances 0.000 title abstract description 4
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims abstract description 69
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 52
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- 229910052757 nitrogen Inorganic materials 0.000 claims description 26
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- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 5
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 4
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- RHZUVFJBSILHOK-UHFFFAOYSA-N anthracen-1-ylmethanolate Chemical compound C1=CC=C2C=C3C(C[O-])=CC=CC3=CC2=C1 RHZUVFJBSILHOK-UHFFFAOYSA-N 0.000 claims description 3
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- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 5
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Images
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- E—FIXED CONSTRUCTIONS
- E21—EARTH OR ROCK DRILLING; MINING
- E21B—EARTH OR ROCK DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B43/00—Methods or apparatus for obtaining oil, gas, water, soluble or meltable materials or a slurry of minerals from wells
- E21B43/16—Enhanced recovery methods for obtaining hydrocarbons
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
- C12P5/023—Methane
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- E21—EARTH OR ROCK DRILLING; MINING
- E21B—EARTH OR ROCK DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B43/00—Methods or apparatus for obtaining oil, gas, water, soluble or meltable materials or a slurry of minerals from wells
- E21B43/006—Production of coal-bed methane
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
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Abstract
The invention belongs to the technical field of coal bed gas yield increase, and particularly relates to a method for improving the yield of biological coal bed gas through feed supplement fermentation. According to the invention, by collecting heterotopic enrichment culture of microbial floras of coal bed gas in a coal bed, nutrients are supplemented in the middle or later period of generation of the biological coal bed gas, so that the yield of the coal bed gas is increased. The invention selects the change of nutrient substances or flora as the time point of adding the nutrient substances, and has very strong pertinence. Compared with the method for directly producing methane by using coalbed indigenous bacteria, the method for improving the yield of the biological coalbed methane by the fed-batch fermentation has the advantages of high gas production amount and prolonged gas production time.
Description
Technical Field
The invention belongs to the technical field of coal bed gas yield increase, and particularly relates to a method for improving the yield of biological coal bed gas through feed supplement fermentation.
Background
Coal Bed Methane (CBM) is a methane gas that is present in coal seams and releases only half of the CO when burning coal compared to burning coal2The emission of CO and NOx is reduced by 80%. As a clean energy source, coal bed gas has attracted attention and attention in many countries. Because the natural gas content in our country is low, the exploitation of coal bed gas is particularly important as a substitute energy of natural gas. Geological data accumulated over the past three decades indicate that biogenic gas is an important source of coal bed methane. The generation of secondary biogenic gas from coal, which typically occurs in shallow layers at temperatures below 100 ℃, is a result of microbial community activity after coal coalification. However, the process of biologically generating coal bed gas is slow, so that the application of the coal bed gas is restricted. Therefore, how to improve the efficiency of producing coal bed gas by microorganisms is a problem to be solved at present.
Coal, under certain conditions, can produce new methane during the degradation process of microorganisms. Because coal is a very complex heterocyclic macromolecule, the coal needs to be gradually degraded under the combined action of various coal bed microorganisms to finally generate coal bed gas, and on the basis, a method for degrading coal by microorganisms in an anaerobic manner is generally adopted at present, and the characteristic of methane production by methanogens in an anaerobic manner is combined to realize the microbial increase of the coal bed gas. For example, CN201210035682.0 discloses a method for increasing the yield of coal bed gas by using exogenous microorganisms, which specifically comprises activating indigenous bacteria in a coal bed by using exogenous bacteria, and degrading organic matters on the surface of coal to generate methane; CN201610710769.1 discloses a method for improving the yield of coal bed gas by using indigenous bacteria; CN201710721266.9 discloses a method for improving the yield of biological coal bed gas by using indigenous fungi.
Disclosure of Invention
Aiming at the problems, the invention provides a method for improving the yield of biological coal bed gas by feed fermentation.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for improving the yield of biological coal bed gas by feed fermentation comprises the following steps:
step 1, collecting coal dust and coal seam effluent samples of a plurality of coal seam gas wells, filling nitrogen to isolate oxygen and wrapping the coal seam effluent samples with aluminum foil paper for later use;
step 2, adding the collected coal dust and coal bed effluent samples into an enrichment medium, acclimating, culturing and screening the floras with the highest coal bed gas yield of coal dust per unit mass as a bacterial source for biological coal bed gas production;
step 3, adding the flora screened in the step 2 into 900mL of coal bed methane production medium according to the volume ratio of 10% for fermentation, and adding 0.05-0.2% w/w of nutrient substances for biological production of coal bed methane when the nitrogen content or phosphorus content in the biological coal bed methane fermentation liquid is reduced to the basic level nitrogen content or phosphorus content or the consumption is finished, or the content of methanogenic archaea is reduced;
and 4, extracting microbial DNA from the collected coal dust and coal seam effluent samples and the flora in the step 2, and performing microbial flora structure analysis by a PCR-DGGE method.
Further, the enrichment medium in the step 2 is composed of 5% of pulverized coal as a unique carbon source, 0.2% of a nitrogen source, 0.4% of phosphorus and potassium and 1% of a trace element solution.
Further, the pulverized coal is any one of anthracite, bituminous coal or lignite; the granularity of the coal powder is 60-160 meshes, and the coal powder needs to be dried for 6 months;
the nitrogen source is an organic nitrogen source or an inorganic nitrogen source;
the organic nitrogen source is any one of yeast powder, peptone, soybean hydrolysate or corn steep liquor dry powder;
the inorganic nitrogen source is any one of ammonium sulfate, ammonium chloride, ammonium nitrate or ammonium hydrogen phosphate;
the phosphorus and potassium are any one of potassium phosphate, monopotassium phosphate, dipotassium hydrogen phosphate or potassium nitrate.
Still further, the trace elements are selected from nitrilotriacetic acid 1.5g and CaCl2 0.1g、MgSO4·7H2O 3.0g、H3BO30.05g、FeSO4 0.1g、NaCl 1.0g、CoCl2 0.1g、MnSO4 0.5g、ZnSO4 0.1g、NaMO4 0.05g、A1K(SO4)2 0.01g、NiCl2 0.1g、CuSO40.01 g.
Further, the coal bed methane culture medium in the step 3 is: adding yeast extract 0.5g and K into 1L deionized water2HPO4 2.9g、KH2PO4 1.5g、NH4Cl 1.8g、MgCl20.4g, 3g of cysteine, 2mL of 0.2% resazurin, and 10mL of a trace element solution, and the pH was 7.0.
Further, the base level of nitrogen content or phosphorus content in step 3: the nitrogen content refers to the content of amino, nitro and nitroso salts in the coal seam, and the phosphorus content refers to the content of phosphate in the coal seam.
Further, the methanogenic archaea is Methanosarcina, Methanobacterium or Methanoculleus.
Further, the content of the archaeological methane-producing bacteria in the step 3 is reduced in a production period, the content of the archaeological methane-producing bacteria is increased and then reduced, and the content reduction is compared with the highest content.
Further, the nutrient substance in the step 3 is one or two of organic nitrogen, inorganic nitrogen and inorganic phosphorus, including a nitrogen source and a phosphorus source;
the organic nitrogen is any one of yeast powder, peptone, soybean hydrolysate or corn steep liquor dry powder;
the inorganic nitrogen is any one of ammonium sulfate, ammonium chloride, ammonium nitrate or ammonium hydrogen phosphate;
the inorganic phosphorus is any one of potassium phosphate, monopotassium phosphate, dipotassium phosphate or potassium nitrate.
Further, the PCR-DGGE method in the step 4 is a PCR-DGGE analysis method with species standard introduced during electrophoresis analysis.
Compared with the prior art, the invention has the following advantages:
the coal geological microorganisms are in a severe environment and are in an inactive state, and can be stimulated by some outside world to perform metabolic activity better. In terms of biological coal bed gas production, the process is a process for producing methane by mixed bacteria fermentation, and in comparison, a carbon source, namely coal is excessive, and other nutrient substances, such as nitrogen sources, phosphorus, potassium and the like, are insufficient, so that the carbon source, namely the coal is required to be supplemented in time to be beneficial to the growth of microorganisms, but the selection time of the supplement is very critical, and the growth and the gas production are greatly influenced by early, late and insufficient amount. The invention selects the change of nutrient substances or flora as the adding time point, and has very strong pertinence. Compared with the method for directly producing methane by using coalbed indigenous bacteria, the method for improving the yield of the biological coalbed methane by the fed-batch fermentation has the advantages of high gas production amount and prolonged gas production time.
Drawings
FIG. 1 is a diagram showing the condition of coal bed methane produced by microorganisms in a coal bed after nitric acid is supplemented under the experimental conditions of the invention.
Detailed Description
Example 1
In an anaerobic glove box, 50mL of a coal bed output water sample and 2g of a coal sample are added into a 500mL anaerobic bottle, 200mL of a sterilization enrichment culture medium is added, then 0.04% of L-cysteine and 20g of lignite powder which are introduced with nitrogen are added, the mixture is gently mixed, the anaerobic bottle is rapidly sealed, and the mixture is subjected to standing culture at the temperature of 25 ℃. During the period, a sterile syringe is inserted from the top of the anaerobic bottle every week for automatic collection of gas, 0.5mL of the collected gas is taken for gas chromatography analysis, and the relative content of methane generation is calculated. (enrichment culture screening bacteria)
Enrichment medium
2g of yeast extract and K are added into 1L of deionized water2HPO4 2.9g、KH2PO4 1.5g、NH4Cl 1.8g、MgCl20.4g, 3g of cysteine, 2mL of resazurin (0.2%), 10mL of a trace element solution with a pH of 7.0, and 5mL of a vitamin solution.
The formula of the trace element solution is as follows: nitrilotriacetic acid 1.5g, CaCl2 0.1g、MgSO4·7H2O 3.0g、H3BO30.05g、FeSO4 0.1g、NaCl 1.0g、CoCl2 0.1g、MnSO4 0.5g、ZnSO4 0.1g、NaMO4 0.05g、A1K(SO4)2 0.01g、NiCl2 0.1g、CuSO4 0.01g。
1L vitamin solution comprises: biotin 2mg, folic acid 2mg, B610 mg, B25 mg, B15 mg, nicotinic acid 5mg, B120.1mg, lipoic acid 5mg, p-aminobenzoic acid 5 mg.
Example 2
Adding 25g pulverized lignite, 350mL of production medium and 0.408mg of Resazurin in a 1L anaerobic bottle, and adding 1 × 10 parts of5Pa sterilizing for 30 min. Adding 800 microliter of sterile 20 percent L-cysteine into a 1L anaerobic bottle in an anaerobic glove box, and then continuously introducing high-purity N into the anaerobic bottle2Until the color of the culture medium is nearly colorless. In an anaerobic glove box, 50mL of the culture solution of example 1 was inoculated into the 1L anaerobic flask described above. Sealing each anaerobic bottle for gas production experiments, and performing standing culture in an anaerobic box for 1-3 months. During the culture period, samples were taken every other week, and the content of archaea methanes in the culture broth was measured, together with NH, and4 +、NO3 -and PO4 3-When the content of Methanosarccina and Methanobacterium in the archaeolhamus methanetus flora is reduced, or when the content of the ions in the archaeolhamus methanetus flora is reduced to a basic level (the content of coal bed ions) or the ions are completely consumed, 0.2% of yeast powder, ammonium sulfate and 0.4% of potassium phosphate are supplemented, a sterile syringe is inserted from the top of an anaerobic bottle every week in the period of time to automatically collect gas, 0.5mL of collected gas is taken to perform gas chromatography analysis, and the relative content of generated methane is calculated. Meanwhile, 5-10 mL of culture solution is taken for qualitative and quantitative analysis of flora.
Coal bed gas culture medium:
adding yeast extract 0.5g and K into 1L deionized water2HPO4 2.9g、KH2PO4 1.5g、NH4Cl 1.8g、MgCl20.4g, 3g of cysteine, 2mL of resazurin (0.2%), and 10mL of a trace element solution, with a pH of 7.0.
The formula of the trace element solution is as follows: nitrilotriacetic acid 1.5g, CaCl2 0.1g、MgSO4·7H2O 3.0g、H3BO30.05g、FeSO4 0.1g、NaCl 1.0g、CoCl2 0.1g、MnSO4 0.5g、ZnSO4 0.1g、NaMO4 0.05g、A1K(SO4)2 0.01g、NiCl2 0.1g、CuSO4 0.01g。
Example 3
Adding 25g bituminous coal powder, 350mL of production-period culture medium and 0.408mg of Resazurin in a 1L anaerobic bottle, and adding 1 × 105Pa sterilizing for 30 min. Adding 800 microliter of sterile 20 percent L-cysteine into a 1L anaerobic bottle in an anaerobic glove box, and then continuously introducing high-purity N into the anaerobic bottle2Until the color of the culture medium is nearly colorless. In an anaerobic glove box, 50mL of the culture solution of example 1 was inoculated into the 1L anaerobic flask described above. Sealing each anaerobic bottle for gas production experiments, and performing standing culture in an anaerobic box for 1-3 months. During the culture period, samples were taken every other week, and the content of archaea methanes in the culture broth was measured, together with NH, and4 +、NO3 -and PO4 3-When the content of Methanosarccina and Methanobacterium in the archaeolhamus methanetus flora is reduced, or when the content of the ions in the archaeolhamus methanetus flora is reduced to a basic level (the content of ions in a coal bed) or the ions are completely consumed, 0.2% of peptone, ammonium chloride and 0.4% of potassium dihydrogen phosphate are supplemented, a sterile syringe is inserted from the top of an anaerobic bottle every week to automatically collect gas, 0.5mL of the collected gas is taken to perform gas chromatography analysis, and the relative content of methane generation is calculated. Meanwhile, 5-10 mL of culture solution is taken for qualitative and quantitative analysis of flora.
Coal bed gas culture medium:
adding yeast extract 0.5g and K into 1L deionized water2HPO4 2.9g、KH2PO4 1.5g、NH4Cl 1.8g、MgCl20.4g, 3g of cysteine, 2mL of resazurin (0.2%), and 10mL of a trace element solution, with a pH of 7.0.
The formula of the trace element solution is as follows: nitrilotriacetic acid 1.5g, CaCl2 0.1g、MgSO4·7H2O 3.0g、H3BO30.05g、FeSO4 0.1g、NaCl 1.0g、CoCl2 0.1g、MnSO4 0.5g、ZnSO4 0.1g、NaMO4 0.05g、A1K(SO4)2 0.01g、NiCl2 0.1g、CuSO4 0.01g。
Example 4
Adding 25g anthracite coal powder, 350mL production period culture medium and 0.408mg resazurin, 1 × 10mg into a 1L anaerobic bottle5Pa sterilizing for 30 min. Adding 800 microliter of sterile 20 percent L-cysteine into a 1L anaerobic bottle in an anaerobic glove box, and then continuously introducing high-purity N into the anaerobic bottle2Until the color of the culture medium is nearly colorless. In an anaerobic glove box, 50mL of the culture solution of example 1 was inoculated into the 1L anaerobic flask described above. Sealing each anaerobic bottle for gas production experiments, and performing standing culture in an anaerobic box for 1-3 months. During the culture period, samples were taken every other week, and the content of archaea methanes in the culture broth was measured, together with NH, and4 +、NO3 -and PO4 3-When the content of Methanosarccina and Methanobacterium in the archaeolhamus methanesulphone flora is reduced, or when the content of ions in the archaeolhamus methanesulphone flora is reduced to a basic level (the content of ions in a coal bed) or the ions are completely consumed, 0.2% of corn steep liquor dry powder, ammonium nitrate and 0.4% of dipotassium hydrogen phosphate are supplemented, a sterile syringe is inserted from the top of an anaerobic bottle every week to automatically collect gas, 0.5mL of collected gas is taken to perform gas chromatography analysis, and the relative content of generated methane is calculated. Meanwhile, 5-10 mL of culture solution is taken for qualitative and quantitative analysis of flora. As shown in figure 1, the condition of coal bed methane produced by coal bed microorganisms after nitric acid is supplemented is shown.
Coal bed gas culture medium:
adding yeast extract 0.5g and K into 1L deionized water2HPO4 2.9g、KH2PO4 1.5g、NH4Cl 1.8g、MgCl20.4g, 3g of cysteine, 2mL of resazurin (0.2%), and 10mL of a trace element solution, with a pH of 7.0.
The formula of the trace element solution is as follows: nitrilotriacetic acid 1.5g, CaCl2 0.1g、MgSO4·7H2O 3.0g、H3BO30.05g、FeSO4 0.1g、NaCl 1.0g、CoCl2 0.1g、MnSO4 0.5g、ZnSO4 0.1g、NaMO4 0.05g、A1K(SO4)2 0.01g、NiCl2 0.1g、CuSO4 0.01g。
Example 5
The primers for PCR amplification of 16S rRNA genes of bacteria and archaea are BAC-338F (plus GC clamp)/BAC-518R and AR-344F (plus GC clamp)/AR-519R respectively. And (3) PCR reaction system: pre-denaturation at 95 deg.C for 5min, 30s at 95 deg.C, 30s at 55 deg.C, 30s at 72 deg.C, 30 cycles, 10min at 72 deg.C, and preservation at 10 deg.C.
The conditions for the DGGE analysis of bacteria and archaea were the same. DGGE gel is polyacrylamide gel with the concentration of 10% (v/v), and the denaturation gradient range of the gel is 40-60%. The electrophoresis condition is that the electrophoresis is carried out for 13 hours at the constant temperature of 60 ℃ and the voltage of 85V. After the electrophoresis is finished, the gel is taken down, stained in 4S red plus nucleic acid stain for 15min, then placed into pure water, and decolorized for 10min by a decolorization shaking table. And observing and photographing under a gel image analysis system to obtain a DGGE map. DGGE map result analysis adopts Quantity One software.
Species qualitative analysis: after the sample is subjected to PCR amplification, a product and the coal bed microorganism species DGGE Marker (CN 2017103488119.0, CN201710349248.2) are simultaneously loaded on polyacrylamide denatured gel, imaging is carried out after electrophoresis, and a sample DNA fragment at the same position with the Marker represents a species the same as the Marker.
The embodiments are described in detail, but the present invention is not limited to the above embodiments and examples, and various changes and modifications within the knowledge of those skilled in the art may be made without departing from the spirit of the present invention, and the changes and modifications fall within the scope of the present invention.
Claims (4)
1. A method for improving the yield of biological coal bed gas by feed fermentation is characterized by comprising the following steps: the method comprises the following steps:
step 1, collecting coal dust and coal seam effluent samples of a plurality of coal seam gas wells, filling nitrogen to isolate oxygen and wrapping the coal seam effluent samples with aluminum foil paper for later use;
step 2, adding the collected coal dust and coal bed effluent samples into an enrichment medium, acclimating, culturing and screening the floras with the highest coal bed gas yield of coal dust per unit mass as a bacterial source for biological coal bed gas production;
step 3, adding the flora screened in the step 2 into 900mL of coal bed methane production medium according to the volume ratio of 10% for fermentation, and adding 0.05-0.2% w/w of nutrient substances for biological production of coal bed methane when the nitrogen content or phosphorus content in the biological coal bed methane fermentation liquid is reduced to the basic level nitrogen content or phosphorus content or the consumption is finished, or the content of methanogenic archaea is reduced;
the coal bed gas culture medium comprises: adding yeast extract 0.5g and K into 1L deionized water2HPO42.9g、KH2PO41.5g、NH4Cl 1.8g、MgCl20.4g, 3g of cysteine, 2mL of 0.2% resazurin and 10mL of trace element solution, wherein the pH value is 7.0;
the methanogenic archaea is Methanosarcina, Methanobacterium or Methanovulleus;
nitrogen or phosphorus content of the base level: the nitrogen content refers to the content of amino, nitro and nitroso salts in the coal seam, and the phosphorus content refers to the content of phosphate in the coal seam;
the content of the archaea capable of producing methane is reduced in a production period, the content of the archaea capable of producing methane is increased firstly and then reduced, and the content reduction is compared with the highest content;
the nutrient is one or two of organic nitrogen, inorganic nitrogen and inorganic phosphorus, including a nitrogen source and a phosphorus source; the organic nitrogen is any one of yeast powder, peptone, soybean hydrolysate or corn steep liquor dry powder; the inorganic nitrogen is any one of ammonium sulfate, ammonium chloride, ammonium nitrate or ammonium hydrogen phosphate; the inorganic phosphorus is any one of potassium phosphate, monopotassium phosphate, dipotassium phosphate or potassium nitrate;
step 4, extracting microbial DNA from the collected coal dust and coal seam effluent sample and the flora in the step 2, and performing microbial flora structure analysis by a PCR-DGGE method; the PCR-DGGE method is a PCR-DGGE analysis method with species standard introduced during electrophoretic analysis.
2. The method for improving the yield of biological coal bed methane through fed-batch fermentation according to claim 1, wherein the method comprises the following steps: the enrichment medium in the step 2 is composed of 5% of coal dust as a unique carbon source, 0.2% of nitrogen source, 0.4% of phosphorus and potassium and 1% of trace element solution.
3. The method for improving the yield of biological coal bed methane through fed-batch fermentation according to claim 2, wherein the method comprises the following steps: the pulverized coal is any one of anthracite, bituminous coal or lignite; the granularity of the coal powder is 60-160 meshes, and the coal powder needs to be dried for 6 months;
the nitrogen source is an organic nitrogen source or an inorganic nitrogen source;
the organic nitrogen source is any one of yeast powder, peptone, soybean hydrolysate or corn steep liquor dry powder;
the inorganic nitrogen source is any one of ammonium sulfate, ammonium chloride, ammonium nitrate or ammonium hydrogen phosphate;
the phosphorus and potassium are any one of potassium phosphate, monopotassium phosphate, dipotassium hydrogen phosphate or potassium nitrate.
4. The method for improving the yield of biological coal bed methane through fed-batch fermentation according to claim 2, wherein the method comprises the following steps: the trace elements comprise nitrilotriacetic acid 1.5g and CaCl20.1g、MgSO4·7H2O 3.0g、H3BO30.05g、FeSO40.1g、NaCl 1.0g、CoCl20.1g、MnSO40.5g、ZnSO40.1g、NaMO40.05g、A1K(SO4)20.01g、NiCl20.1g、CuSO40.01 g.
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