CN111137975A - Salt-tolerant anaerobic ammonium oxidation sludge domestication method based on inhibition dynamics law - Google Patents
Salt-tolerant anaerobic ammonium oxidation sludge domestication method based on inhibition dynamics law Download PDFInfo
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Abstract
The invention discloses a method for domesticating salt-tolerant anaerobic ammonium oxidation sludge based on an inhibition dynamics law, which comprises the following steps: (1) inoculating anaerobic ammonium oxidation granular sludge in a reactor, pumping inlet water, and NH in the inlet water4 +-N and NO2 ‑The mass concentration ratio of-N is 1:1, and the operation is carried out to be stable under the conditions of anaerobic property, light shielding and 30-36 ℃; (2) adding salt ions into the influent water, increasing the concentration of the salt ions by stages by adopting a strategy based on the law of inhibition kinetics, operating each domestication stage for the same time, continuously increasing the tolerance of the anammox bacteria to salinity and enriching the salt-tolerant bacteria until the concentration of the salt ions in the influent water reaches the target concentration, and completing the domestication of the salt-tolerant anammox sludge. The anaerobic ammonia oxidation sludge obtained by the domestication method can keep higher denitrification performance under a high salinity level, has tolerance capability to a common salinity level, and is beneficial to the practical application of an anaerobic ammonia oxidation process.
Description
Technical Field
The invention relates to the technical field of sludge domestication, in particular to a method for domesticating salt-tolerant anaerobic ammonium oxidation sludge based on an inhibition dynamics law.
Background
In order to alleviate the crisis of fresh water resources in the world, seawater is increasingly applied to the development of life and industry of people, and the salinity of municipal sewage is greatly increased. Furthermore, the rapid development of fisheries, marinades, and leather manufacturing industries in recent years has also produced large amounts of salt-containing wastewater. According to incomplete statistics, 5% of industrial wastewater produced in the world is saline wastewater. The increase in salinity also presents challenges in the field of biological treatment of wastewater. High salinity causes an increase in osmotic pressure in the aqueous environment, which is also the primary cause of high salinity poisoning of water treatment microorganisms.
Anammox refers to a biological reaction in which ammonia is oxidized to nitrogen using nitrite as an electron acceptor under anaerobic conditions. Compared with the traditional nitrification-denitrification biological denitrification process, the anaerobic ammonia oxidation process does not need aeration and an additional carbon source while maintaining high-efficiency denitrification. This makes the anammox process considered a promising biological denitrification process. In addition, the anaerobic ammonia oxidation process also shows great potential in the treatment of high-salinity nitrogen-containing wastewater. The anaerobic ammonium oxidation bacteria of CandidatusScalindua can even have salinity as high as 204g NaCl L-1And (3) survival.
The salt tolerance domestication refers to a microorganism culture process of slowly increasing the salinity in the sewage and continuously increasing the osmotic pressure to a desired concentration. During the acclimation process, the salt tolerance of the microorganisms is gradually increased, and the microorganisms with low salinity tolerance are gradually eliminated. At present, most of the domestication methods are adopted to realize the denitrification treatment of the saline wastewater by the anaerobic ammonia oxidation process. In traditional acclimation, the salinity is generally increased in proportion to the acclimation time. However, such domestication does not take the bearing capacity of the microorganisms into full consideration, and does not necessarily lead to optimal domestication results, or even to domestication failure. In practice, maintaining a uniform increase in salinity-inhibiting degree of microorganisms rather than salt ion concentration over acclimation time may lead to better acclimation results.
Disclosure of Invention
Aiming at the defects in the field, the invention provides a method for domesticating salt-tolerant anammox sludge based on an inhibition dynamics law, which ensures that the inhibition degree of salinity on microorganisms is uniformly increased along with the domestication time in the domestication process by combining the inhibition dynamics law of salinity on anammox bacteria, thereby successfully domesticating the salt-tolerant anammox sludge. The invention provides a new method and a new thought for the denitrification process of high-salinity wastewater based on an anaerobic ammonia oxidation process.
A method for domesticating salt-tolerant anaerobic ammonium oxidation sludge based on an inhibition kinetics law comprises the following steps:
(1) inoculating anaerobic ammonium oxidation granular sludge in a reactor, pumping inlet water, and NH in the inlet water4 +-N and NO2 -The mass concentration ratio of-N is 1:1, and the operation is carried out to be stable under the conditions of anaerobic property, light shielding and 30-36 ℃;
(2) adding salt ions into the influent water, increasing the concentration of the salt ions by stages by adopting a strategy based on the law of inhibition kinetics, operating each domestication stage for the same time, continuously increasing the tolerance of the anammox bacteria to salinity and enriching the salt-tolerant bacteria until the concentration of the salt ions in the influent water reaches the target concentration, and completing the domestication of the salt-tolerant anammox sludge.
The anaerobic ammonia oxidation sludge obtained by the domestication method can keep higher denitrification performance under a high salinity level, has tolerance capability to a common salinity level, and is beneficial to the practical application of an anaerobic ammonia oxidation process.
Preferably, the reactor is an upflow anaerobic sludge blanket reactor, which is beneficial to maintaining the anaerobic state in the whole acclimation process.
Preferably, the concentration of the anaerobic ammonia oxidation granular sludge inoculated in the reactor is 10-15 g-1VSS L-1. VSS is a volatile suspended solid.
Preferably, the dominant genus in the anammox granular sludge is Candidatus Kuenenia.
Preferably, the pH of the influent water is 7.6 to 7.8, which is most suitable for culturing anammox bacteria.
Preferably, the feed water contains (NH)4)2SO4，NaNO2，NaH2PO4，CaCl2·2H2O，MgSO4·7H2O，NaHCO3A microelement I stock solution and a microelement II stock solution;
the stock solution of the trace element I consists of EDTA and FeSO4Preparing;
the microelement II stock solution contains: EDTA, ZnSO4·7H2O，CoCl2·6H2O，MnCl2·4H2O，CuSO4·5H2O，NaMoO4·2H2O，NiCl2·6H2O and H3BO4。
The feed water contains nutrients necessary for the growth of anammox bacteria and is suitable for the growth of anammox bacteria.
Preferably, the hydraulic retention time of the inlet water is 1.5-2.0 h.
In the step (2), the inhibition kinetics law is described by using an equation Y ═ f (X), where Y is the activity of the anammox bacteria, and X is the salt ion concentration, and preferably, the value Y corresponding to the salt ion concentration X in each acclimation stage should be uniformly changed along with the acclimation stage.
Preferably, the equation Y ═ f (x) is a quadratic function.
Preferably, the salt ions are regulated to the target concentration not to exceed 30g L mainly by the addition of NaCl-1And the domestication failure caused by overhigh target concentration of the domestication is prevented. The target concentrations for different salts can be adjusted accordingly.
Preferably, the step (1) is used as a first domestication stage, and the total number of the domestication stages is not less than 5.
Preferably, the operation time of each domestication stage is not less than 14 days, so that the domestication time is not too short, and the domestication result is not ideal.
In a preferred embodiment, the salt ion concentration in the inlet water at different stages is 0.00g L-1、4.83g L-1、10.34gL-1、16.85g L-1、25.00g L-1。
Under the acclimatization method of the invention, when the salt ion concentration reaches the acclimatized target concentration of 25.00g NaCl L-1At this time, the nitrogen removal rate NRR of the reactor reached 2.22. + -. 0.14kg Nm-3d-1Is obviously higher than 1.75 plus or minus 0.23kg N m under the conventional domestication method-3d-1。
Compared with the prior art, the invention has the main advantages that: the tolerance level of the anammox bacteria to salinity is fully considered in the acclimation process, the inhibition pressure generated by the salinity is ensured to be uniformly increased along with the change of the acclimation stage, and a stable external environment is provided for the anammox bacteria, so that a good acclimation result is obtained.
Detailed Description
The invention will be further illustrated with reference to the following specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. The following examples are conducted under conditions not specified, usually according to conventional conditions, or according to conditions recommended by the manufacturer.
(1) An up-flow anaerobic sludge bed reactor with the effective volume of 1.5L is adopted, 0.6L of anaerobic ammonium oxidation granular sludge is inoculated, the reactor is placed in a thermostatic chamber with the temperature of 35 +/-1 ℃, the reactor is operated in an anaerobic and light-proof manner, the hydraulic retention time is 2.0h, the total nitrogen load is adjusted according to the operation condition of the reactor, and in the embodiment, the total nitrogen load is 6.72-3.36 kg Nm-3d-1。
The initial water inlet component is as follows:
wherein, the stock solution of the trace element I comprises the following components:
EDTA 5.00g L-1，
FeSO49.14g L-1。
the stock solution of the trace element II comprises the following components:
the pH of the initial influent water is 7.6 to 7.8.
(2) In the initial feed water (NH)4)2SO4With NaNO2All the concentrations of (A) and (B) are 280mg L-1After culturing for 14 days under the conditions of (1), the total nitrogen removal rate of the reactor reaches 91.5%, and the anammox activity of the anammox sludge in the reactor is determined to be 187.9 +/-5.11 mg of N gVSS-3d-1The dominant strain is Candidatus Kuenenia stuttgartiensis, which shows that the reactor is successfully started and stably operated and has the condition of salinity acclimation.
(3) In this example the salt ions are added in the form of NaCl. In order to determine the inhibition rule of salinity on the anammox bacteria and provide a basis for the acclimation method, firstly, the anammox activity of the unacclimated anammox sludge is measured under different salt ion concentrations, and a quadratic function is adopted to perform kinetic fitting on an experimental result to obtain that the two satisfy an equation (I):
rAA＝0.050c2-4.41c+101.77(I)，
wherein rAA is relative anammox activity, and the unit is percent of anammox activity and initial anammox activity under corresponding salt ion concentration; c is the salt ion concentration in g L-1。
(4) In the embodiment, the acclimatization process comprises 5 stages, each stage is maintained for 14 days, the rAA in the acclimatization process is ensured to be uniformly reduced along with different acclimatization stages, so that the inhibition pressure of salinity on the anaerobic ammonium oxidation bacteria is uniformly increased along with the acclimatization stages, and when the acclimatization target concentration is 25.00g NaCl L-1Then, the salt ion concentrations in the inlet water at different stages are respectively 0.00g L calculated by the equation (I)-1、4.83g L-1、10.34g L-1、16.85g L-1、25.00g L-1。
(5) Control experimental groups were set up to determine what was taken under different acclimatization methodsThe domestication effect obtained by the method is that the domestication process of the control test group also comprises 5 stages, each stage is maintained for 14 days, and the domestication target concentration is also 25.00g NaCl L-1The concentration of the salt ions is increased by adopting an equal concentration gradient, namely the concentration of the salt ions is 0.00g L under different domestication stages respectively-1、6.25g L-1、12.5g L-1、18.75g L-1、25.00g L-1。
(6) Increasing the concentration of salt ions in the reactor to 25.00g NaCl L by using the salt concentration increasing strategies described in (4) and (5), respectively-1After culturing for 14 days under the concentration, measuring related indexes of the reactors and sludge in the reactors, and domesticating the nitrogen removal rate NRR of the reactors under the inhibition dynamics law to be 2.22 +/-0.14 kg Nm-3d-1The anaerobic ammonia oxidation activity of the sludge in the reactor is 41.56 +/-8.74 mg of N gVSS-3d-1While the NRR of the control reactor under the same conditions was only 1.75. + -. 0.23kg NM-3d-1The anaerobic ammonia oxidation activity is 36.55 +/-9.07 mg of N gVSS-3d-1。
In general, the method performs salt tolerance acclimation on the anammox sludge on the basis of the inhibition dynamics law of salinity on the anammox bacteria, fully considers the tolerance level of the anammox bacteria on the salinity in the acclimation process, ensures that the inhibition pressure generated by the salinity is uniformly increased along with the change of the acclimation stage, and obtains a more excellent acclimation result compared with the conventional acclimation method.
The final NaCl concentration was 25.00g L-1The anaerobic ammonium oxidation granular sludge domesticated by the domestication method has excellent denitrification performance and is beneficial to the practical application of the anaerobic ammonium oxidation process.
Further, it will be appreciated that various changes and modifications may be made by those skilled in the art after reading the foregoing description of the invention, and equivalents may fall within the scope of the invention as defined by the appended claims.
Claims (10)
1. A method for domesticating salt-tolerant anaerobic ammonium oxidation sludge based on an inhibition kinetics law is characterized by comprising the following steps:
(1) inoculating anaerobic ammonium oxidation granular sludge in a reactor, pumping inlet water, and NH in the inlet water4 +-N and NO2 -The mass concentration ratio of-N is 1:1, and the operation is carried out to be stable under the conditions of anaerobic property, light shielding and 30-36 ℃;
(2) adding salt ions into the influent water, increasing the concentration of the salt ions by stages by adopting a strategy based on the law of inhibition kinetics, operating each domestication stage for the same time, continuously increasing the tolerance of the anammox bacteria to salinity and enriching the salt-tolerant bacteria until the concentration of the salt ions in the influent water reaches the target concentration, and completing the domestication of the salt-tolerant anammox sludge.
2. The method for acclimatizing salt-tolerant anaerobic ammonium oxidation sludge based on the inhibition kinetics law according to claim 1, wherein the reactor is an upflow anaerobic sludge blanket reactor.
3. The method for acclimatizing salt-tolerant anammox sludge according to claim 1, wherein the concentration of the anammox granular sludge inoculated in the reactor is 10-15 g-1VSS L-1。
4. The method for acclimatizing salt-tolerant anammox sludge according to claim 1, wherein the dominant bacteria in the anammox granular sludge is Candidatus Kuenenia.
5. The method for acclimatizing salt-tolerant anaerobic ammonium oxidation sludge based on the inhibition kinetics law according to claim 1, wherein the pH of the influent water is 7.6-7.8, and the hydraulic retention time is 1.5-2.0 h.
6. The method for acclimatizing salt-tolerant anammox sludge according to claim 1, wherein the inhibition kinetics law is described by using the equation of Y ═ f (X), where Y is the activity of anammox bacteria, X is the salt ion concentration, and the value of Y corresponding to the salt ion concentration X in each acclimatization phase is uniformly changed along with the acclimatization phase.
7. The method for acclimatizing salt-tolerant anaerobic ammonium oxidation sludge according to the inhibition kinetics law of claim 6, wherein the equation Y is a quadratic function F (X).
8. The method for acclimatizing salt-tolerant anammox sludge according to claim 1, wherein the salt ions are added in the form of NaCl, and the target concentration is not more than 30g L-1。
9. The method for acclimatizing the salt-tolerant anammox sludge based on the inhibition kinetics law according to claim 1, wherein the step (1) is used as a first acclimation stage, and the total number of the acclimation stages is not less than 5.
10. The method for acclimatizing salt-tolerant anammox sludge according to the inhibition kinetics law of claim 1 or 9, wherein the operation time of each acclimation stage is not less than 14 days.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112047464A (en) * | 2020-08-04 | 2020-12-08 | 北京工业大学 | Culture method of tolerant low-dose fulvic acid anaerobic ammonium oxidation granular sludge |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6569334B1 (en) * | 1999-06-10 | 2003-05-27 | Bicom Corporation | Method of high-concentration culture of nitrifying bacteria or denitrifying bacteria contained in activated sludge, culture promoter to be used in high-concentration culture method of nitrifying bacteria, and method of weight loss treatment of activated sludge |
| CN101289252A (en) * | 2008-06-03 | 2008-10-22 | 桂林工学院 | Sludge acclimation method for adapting bacteria in activated sludge to high salinity environment |
| JP4903908B1 (en) * | 2011-05-18 | 2012-03-28 | メタウォーター株式会社 | Biological treatment apparatus and method for organic waste |
| CN102952764A (en) * | 2011-08-31 | 2013-03-06 | 中国石油化工股份有限公司 | Culture method of salt-tolerant anaerobic ammonium oxidation bacteria |
| CN102976489A (en) * | 2012-12-21 | 2013-03-20 | 重庆大学 | Method for starting anaerobic ammonium oxidation reactor to treat high-salt nitrogenous wastewater |
| CN103910475A (en) * | 2013-12-30 | 2014-07-09 | 新疆环境工程技术有限责任公司 | Method for domestication of salt-resistant activated sludge |
| WO2015173336A1 (en) * | 2014-05-14 | 2015-11-19 | Yara International Asa | Denitrification of saline industrial waste water |
-
2020
- 2020-01-13 CN CN202010032705.7A patent/CN111137975A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6569334B1 (en) * | 1999-06-10 | 2003-05-27 | Bicom Corporation | Method of high-concentration culture of nitrifying bacteria or denitrifying bacteria contained in activated sludge, culture promoter to be used in high-concentration culture method of nitrifying bacteria, and method of weight loss treatment of activated sludge |
| CN101289252A (en) * | 2008-06-03 | 2008-10-22 | 桂林工学院 | Sludge acclimation method for adapting bacteria in activated sludge to high salinity environment |
| JP4903908B1 (en) * | 2011-05-18 | 2012-03-28 | メタウォーター株式会社 | Biological treatment apparatus and method for organic waste |
| CN102952764A (en) * | 2011-08-31 | 2013-03-06 | 中国石油化工股份有限公司 | Culture method of salt-tolerant anaerobic ammonium oxidation bacteria |
| CN102976489A (en) * | 2012-12-21 | 2013-03-20 | 重庆大学 | Method for starting anaerobic ammonium oxidation reactor to treat high-salt nitrogenous wastewater |
| CN103910475A (en) * | 2013-12-30 | 2014-07-09 | 新疆环境工程技术有限责任公司 | Method for domestication of salt-resistant activated sludge |
| WO2015173336A1 (en) * | 2014-05-14 | 2015-11-19 | Yara International Asa | Denitrification of saline industrial waste water |
Non-Patent Citations (1)
| Title |
|---|
| 齐泮晴等: "盐度对厌氧氨氧化工艺处理含海水污水脱氮特性的影响", 《中国环境科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112047464A (en) * | 2020-08-04 | 2020-12-08 | 北京工业大学 | Culture method of tolerant low-dose fulvic acid anaerobic ammonium oxidation granular sludge |
| CN112047464B (en) * | 2020-08-04 | 2022-03-22 | 北京工业大学 | Culture method of tolerant low-dose fulvic acid anaerobic ammonium oxidation granular sludge |
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Application publication date: 20200512 |