CN1111283A - After-treatment method for producing thuricide suspension - Google Patents
After-treatment method for producing thuricide suspension Download PDFInfo
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- CN1111283A CN1111283A CN 94105201 CN94105201A CN1111283A CN 1111283 A CN1111283 A CN 1111283A CN 94105201 CN94105201 CN 94105201 CN 94105201 A CN94105201 A CN 94105201A CN 1111283 A CN1111283 A CN 1111283A
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Abstract
By means of the said post-treatment of Bacillus thuringiensis suspension, can be produced various suspension, slurry, powder, wettable powder, floating agent, etc. with different virulence titer and bacillus content. It has high crystal and spore recovering rate.
Description
The present invention has separated bacillus thuringiesis (Bacillus thuringiensis is hereinafter to be referred as Bt) solid fermentation and liquid fermentation agent production aftertreatment method for producing, and it belongs to the microbial fermentation technology field.
Known field is directly sprayed Bt solid fermentation thing and is used inconvenience; Dried Bt solid fermentation thing is directly pulverized the preparation pulvis, and pulverizing is difficult, work efficiency is low, energy consumption is big, use still inconvenience.
For reducing Bt liquid fermentation agent production cost, improve the rate of recovery of effective constituents such as gemma and crystal toxin, now how to replace filter press with centrifugal spissated method.Existing centrifugal method, the rate of recovery of effective constituents such as its gemma, crystal is still below 70%.Fermented liquid concentrates through repeated centrifugation repeatedly, though can improve gemma, the crystalline rate of recovery, energy consumption strengthens, and causes that easily gemma sprouts and living contaminants.For preventing that gemma is sprouted and living contaminants in the course of processing, need in fermented liquid, to add hydrochloric acid and carry out acidification, the pH value that makes fermented liquid is between the 4-6.
How Bt pulvis or wettable powder production make filler (or claiming carrier) with light calcium carbonate or talcum powder, diatomite, kaolin, wilkinite, chalk soil etc.Bigger by the above-mentioned carrier proportion of what, water-absorbent is relatively poor, fermented liquid or centrifugal concentrated solution through above-mentioned existing carrier absorption, need by just drying processing behind the filter press toward contact, and time of drying is longer, effective ingredient such as gemma, crystal is destroyed bigger in drying course, so pulvis of preparing or wettable powder toxic effect are lower or to contain the living spores amount lower, often is difficult to reach the enterprise product quality technology standard of expection.
The objective of the invention is: use inconvenience and pulverize difficulty, pulverize defectives such as work efficiency is low, energy consumption is big in order to overcome Bt solid fermentation thing, and the formulations such as pulvis, wettable powder, granule, leafing agent that Bt solid fermentation thing can be prepared into suspension agent, paste and high toxic effect or high bacteria containing amount.Simultaneously can improve the gemma and the crystalline rate of recovery in the Bt liquid fermentation agent production aftertreatment course of processing, reduce production costs and energy consumption.Reach the purpose that improves Bt microbial inoculum toxicity evaluation or bacteria containing amount.
For realizing that the measure that purpose of the present invention is taked is:
With the solid fermentation thing of fermentation ends with 10-30 times of water, repeatedly wash, filter (100-300 eye mesh screen) through 1-5 time and be prepared into the Bt bacteria suspension, by known Bt liquid fermenting aftertreatment ordinary method or through the post-treating method processing that this patent has improved, Bt solid fermentation thing can be prepared into formulations such as suspension agent (or claiming aqua or emulsion) or paste or high toxic effect pulvis, wettable powder, granule, leafing agent then.
2. in the bacteria suspension of above-mentioned Bt solid fermentation preparation or Bt liquid fermentation liquid, add 0.05-4% water purification agent-aluminum potassium sulfate (be alum, KAl(SO
4)
212H
2O), replace part or all of hydrochloric acid bacteria suspension or fermented liquid are carried out acidification, when making its pH value reach 4-6, gemma in the above-mentioned bacterium liquid and the automatic aggegation of crystal toxin meeting, be the flower-shaped precipitated and separated of egg, centrifugal or leave standstill processing such as natural subsidence concentrates and can be prepared into formulations such as Bt suspension agent or paste through routine again.
3. light than water with proportion, perlite that water-absorbent is stronger or silicon stone replace existing carriers such as lime carbonate, diatomite, join in the above-mentioned centrifugal or natural subsidence concentrated solution, after whip attachment 5-30 minute, again through air stream drying, pulverizing or processing such as do not pulverize, or, can prepare toxic effect or bacteria containing amount and adopt the high formulations such as pulvis, wettable powder, granule and leafing agent of existing carrier such as lime carbonate, diatomite by the conventional post-treating method processing of existing Bt liquid fermenting.
4. replace drying meanss such as existing oven dry or centrifugal spray drying with air-flow drying method.
The present invention compared with prior art has following advantage:
1.Bt the solid fermentation thing is prepared into bacteria suspension through washing, filtration, the formulations such as suspension agent, paste and wettable powder of the post-treating method preparation that has improved by the conventional post-treating method of Bt liquid fermenting or this patent then, easy to use, pulverize easily, and can prepare toxic effect or bacteria containing amount pulvis or granule than 10 to 20 times of solid fermentation object heights.
2. paste can carry out low dose (50 gram) the quantitative packing of plastics film, and packing charge is adorned low than the plastics kettle of Bt suspension agent heavy dose of (500 grams or 1000 grams), and can make things convenient for the field quantitatively to use, and the sanitas consumption is little simultaneously, and cost is low.
3. replace hydrochloric acid with aluminum potassium sulfate (alum) replacement or part fermented liquid or bacteria suspension are carried out acidification, can prevent that not only gemma is sprouted and living contaminants in the aftertreatment course of processing, and gemma or crystal in fermented liquid or the bacteria suspension are taken place from coagulation phenomena, its settling ratio is strengthened, and settling velocity is accelerated.Energy consumption descends, and the effective constituent rate of recovery such as gemma and crystal increase, and the production cycle shortens.
4. because perlite, silicon stone proportion are light than water, and carrier proportions such as light calcium carbonate, diatomite are heavy than water, the former is to the adsorptive power of water than the big 2-6 of the latter doubly in addition, so use perlite or silicon masonry carrier, generally no longer need just can carry out air stream drying or oven dry processing through filter press, its rate of drying is than existing carrier block such as lime carbonate or diatomite, and its bacteria containing amount of prepared microbial inoculum or toxicity evaluation are with the microbial inoculum height of existing preparing carriers such as lime carbonate.Perlite or silicon stone also can be used as the carrier of leafing agent in addition, and existing carriers such as lime carbonate, diatomite then can not be as the carrier of leafing agent.
5. air stream drying is compared with centrifugal spray drying, and the former has advantages such as reduced investment, work efficiency height, production cost be low; Compare with oven drying method, air stream drying is fast, work efficiency is high, and little to effective constituents such as crystal toxin and gemma destruction, product virulence advantages of higher.
The present invention is described in further detail below in conjunction with Bt solid fermentation aftertreatment embodiment:
1. solid fermentation method: the method for the patented technology of getting permission by us (patent No. 88010450.8) is carried out.
2. bacteria suspension preparation: with the Bt solid fermentation thing that the ferments cloth bag of packing into, tighten sack, put into the inherent 3-20 times of water washing of industry washer or stirred pot 1-30 minute, after the drying, add the washing bacterium with the time according to the above ratio again, so repeat to wash bacterium 0 to 5 time, collect respectively or merge each time and wash bacterium water, the filtrate by the 100-300 eye mesh screen is the Bt bacteria suspension.Maybe this fermented product is directly put into the agitator that 3-20 times of water is housed and stirred 1-30 minute, add water and churning time after the filtration more according to the above ratio and repeat to wash bacterium 0 to 5 time, it is washed bacterium water and is the Bt bacteria suspension.
3. bacteria suspension acidification: in above-mentioned bacteria suspension, add the 0.05-4% aluminum potassium sulfate, stir after 1-30 minute, make its pH value reach 3.5-6.0.If do not reach above-mentioned pH value requirement, can add appropriate hydrochloric acid and make it reach above-mentioned pH value requirement.Leave standstill a moment, can see the floss precipitation.
4.Bt the preparation of suspension agent or paste: the Bt bacteria suspension is after above-mentioned acidification, can carry out centrifugal concentrate or standing sedimentation concentrates, at centrifugal concentrated solution or bacterium mud or leave standstill add in lower floor's thing of natural layering auxiliary agents such as sanitas, spreader-sticker and tackiness agent stir the back Bt suspension agent or paste.
5. pulvis preparation: with perlite or silicon stone by 1:2-7(weight) than adsorbing in adding above-mentioned centrifugal concentrated solution (being bacterium mud) or the natural subsidence concentrated solution, and stir, carry out rapid drying through Q type or QG type or FG type or JG type pneumatic conveying dryer again, be Bt granule or leafing agent; If be the Bt pulvis through operations such as crushing screenings again.The air stream drying operating parameters is as follows:
1. be adsorbed with the perlite of Bt or the first moisture of silicon stone :≤60%, whole moisture :≤4%
2. drying temperature is 140 ± 10 ℃, and the outlet material temperature is 50 ± 10 ℃.
6. wettable powder: the above-mentioned centrifugal concentrated solution (being bacterium mud) or the natural subsidence concentrated solution that perlite or silicon stone are added in advance auxiliary agents such as spreader-sticker, wetting agent by the absorption of 1:2-7 weight ratio, through air stream drying, after be granule or leafing agent, crushing screening is wettable powder.
Claims (5)
1, a kind of bacillus thuringiesis (Bacillus thuringiensis) microbial inoculum is produced post-treating method, it is characterized in that: the solid fermentation thing can be prepared into the Bt bacteria suspension, be prepared into Bt suspension agent, paste and high toxic effect or high bacteria containing amount pulvis, wettable powder then; Granule and leafing agent;
2, by the water purification agent in the described production aftertreatment of claim 1, it is characterized in that: in Bt solid fermentation bacteria suspension or liquid fermentation liquid, add 〉=0.05 to 4% water purification agent-aluminum potassium sulfate (KAl(SO
4)
212H
2O is commonly called as alum), replace part or all of hydrochloric acid and carry out acidification, can make that effective constituent such as gemma and crystal toxin is the flower-shaped coagulation sedimentation layering of egg in bacterium suspension or the fermented liquid;
3, by used carrier in the described production aftertreatment of claim 1, it is characterized in that: with perlite or silicon masonry carrier, not only can be prepared into leafing agent, and pulvis or the wettable powder or the granule of preparation, its bacteria containing amount or toxicity evaluation are higher;
4, by the drying treatment in the described production aftertreatment of claim 1, it is characterized in that: use pneumatic conveying dryer replacement baking oven (room) or centrifugal spray drying equipment to carry out drying treatment, its dry humidity is 〉=130 to 150 ℃, the outlet material temperature is 〉=40 to 60 ℃, be 〉=1 to 5 second each time of drying, it is handled through 〉=1 to 5 time, and the whole moisture of material to be dried of initial moisture content≤60% is dropped to≤4%;
5, by the described production post-treating method of claim 1, it is characterized in that: using Bt solid fermentation thing can be prepared into paste, its wrapping material can be with Plastic film Bag or aluminum foil bag or tin film bag splitting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 94105201 CN1111283A (en) | 1994-05-06 | 1994-05-06 | After-treatment method for producing thuricide suspension |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 94105201 CN1111283A (en) | 1994-05-06 | 1994-05-06 | After-treatment method for producing thuricide suspension |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1111283A true CN1111283A (en) | 1995-11-08 |
Family
ID=5031960
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 94105201 Pending CN1111283A (en) | 1994-05-06 | 1994-05-06 | After-treatment method for producing thuricide suspension |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1111283A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102027999A (en) * | 2010-11-18 | 2011-04-27 | 黑龙江大学 | Bacillus thuringiensis wettable powder containing pullulan polysaccharide and preparation method thereof |
-
1994
- 1994-05-06 CN CN 94105201 patent/CN1111283A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102027999A (en) * | 2010-11-18 | 2011-04-27 | 黑龙江大学 | Bacillus thuringiensis wettable powder containing pullulan polysaccharide and preparation method thereof |
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