CN111117887A - Zebra fish embryo culture device - Google Patents
Zebra fish embryo culture device Download PDFInfo
- Publication number
- CN111117887A CN111117887A CN202010026609.1A CN202010026609A CN111117887A CN 111117887 A CN111117887 A CN 111117887A CN 202010026609 A CN202010026609 A CN 202010026609A CN 111117887 A CN111117887 A CN 111117887A
- Authority
- CN
- China
- Prior art keywords
- bottle cap
- culture device
- bottle
- embryo culture
- filter membrane
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 20
- 241000252212 Danio rerio Species 0.000 title claims abstract description 18
- 239000012528 membrane Substances 0.000 claims abstract description 19
- 239000007789 gas Substances 0.000 claims abstract description 9
- 239000011148 porous material Substances 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 9
- 241000251468 Actinopterygii Species 0.000 description 7
- 210000002257 embryonic structure Anatomy 0.000 description 6
- 239000000463 material Substances 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a zebra fish embryo culture device, which comprises: the bottle comprises a bottle body, a bottle cap and a filter membrane, wherein the bottle cap is installed at the top of the bottle body, and the filter membrane facilitating the exchange of internal and external gases is arranged on the bottle cap. The invention has simple structure and convenient use, and the filter membrane is arranged on the bottle cap, thereby facilitating the exchange of internal and external gases in the culture process.
Description
Technical Field
The invention belongs to the technical field of fish embryo culture devices, and particularly relates to a zebra fish embryo culture device.
Background
At present, a uniform fish embryo culture device is not standardized, most of fish embryos are cultured through a cell plate, but the fish embryos are different from cells, more oxygen is needed in the growth and development process, the number of the individual embryos is far larger than that of the cells, and the volume of the cell plate is not beneficial to carrying out a large amount of culture work. These problems all affect the normal culture of fish embryos.
Therefore, how to provide a zebra fish embryo culture device is a problem which needs to be solved urgently by the technical personnel in the field.
Disclosure of Invention
In view of the above, the invention provides a zebra fish embryo culture device which is simple in structure and convenient to use, and a filter membrane is arranged on a bottle cap, so that the exchange of internal and external gases in the culture process is facilitated.
In order to achieve the purpose, the invention adopts the following technical scheme:
a zebrafish embryo culture device comprising: the bottle comprises a bottle body, a bottle cap and a filter membrane, wherein the bottle cap is installed at the top of the bottle body, and the filter membrane facilitating the exchange of internal and external gases is arranged on the bottle cap.
Preferably, the top of the bottle body is provided with a connecting part, the outer side of the connecting part is provided with an external thread, the inner side of the bottle cap is provided with an internal thread, and the bottle cap is in threaded connection with the connecting part.
Preferably, the bottle body is provided with volume scale marks.
Preferably, the pore diameter of the filter membrane is 0.20-0.25 micrometer.
Preferably, the pore size of the filter membrane is 0.22 microns.
Preferably, the bottle body is made of glass materials.
Preferably, the bottle cap is made of a plastic material.
The invention has the beneficial effects that:
the invention has simple structure and convenient use, and the filter membrane is arranged on the bottle cap, thereby facilitating the exchange of internal and external gases in the culture process; and the volume of the bottle body is large, so that the normal culture of the fish embryo can not be influenced.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a block diagram of the present invention.
Fig. 2 is a schematic structural diagram of the bottle body of the invention.
Fig. 3 is a front view of the bottle cap according to the present invention.
Figure 4 accompanying drawing is a top view of the bottle cap of the present invention.
Wherein, in the figure,
1-a bottle body; 11-a connecting portion; 12-volume graduation mark; 2-bottle cap; 3-filter membrane.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-4, the present invention provides a zebra fish embryo culture device, comprising: the bottle comprises a bottle body 1, a bottle cap 2 and a filter membrane 3, wherein the bottle cap 2 is installed at the top of the bottle body 1, and the filter membrane 3 convenient for gas exchange between the inside and the outside is arranged in the middle of the bottle cap 2.
In another embodiment, the connecting portion 11 is arranged at the top of the bottle body 1, the outer side of the connecting portion 11 is provided with an external thread, the inner side of the bottle cap is provided with an internal thread, and the bottle cap 2 is in threaded connection with the connecting portion 11, so that the bottle cap 2 and the bottle body 1 can be conveniently mounted and dismounted, and a zebra fish embryo can be conveniently placed in the bottle body 1.
In another embodiment, the bottle body 1 is provided with volume graduation marks 12. The user can add an appropriate amount of culture solution according to the indication of the volume scale line 12.
In another embodiment, the pore size of the filter membrane 3 is 0.20 to 0.25 μm.
In another embodiment, the pore size of the filter membrane 3 is 0.22 microns, which facilitates the exchange of internal and external gases during the culture process of the zebra fish embryos and provides enough oxygen for the growth and development process of the zebra fish embryos.
In another embodiment, the bottle body 1 is made of glass materials, so that the manufacturing cost is low, and the practicability is high.
In another embodiment, the bottle cap 2 is made of plastic materials, so that the manufacturing cost is low and the practicability is high.
The invention has simple structure and convenient use, and the filter membrane is arranged on the bottle cap, thereby facilitating the exchange of internal and external gases in the culture process; and the volume of the bottle body is large, so that the normal culture of the fish embryo can not be influenced.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (7)
1. A zebrafish embryo culture device, characterized by comprising: the bottle comprises a bottle body, a bottle cap and a filter membrane, wherein the bottle cap is installed at the top of the bottle body, and the filter membrane facilitating the exchange of internal and external gases is arranged on the bottle cap.
2. The zebrafish embryo culture device of claim 1, wherein the top of the bottle body is provided with a connecting part, the outer side of the connecting part is provided with an external thread, the inner side of the bottle cap is provided with an internal thread, and the bottle cap is in threaded connection with the connecting part.
3. The zebrafish embryo culture device of claim 1 or 2, wherein the bottle body is provided with volume graduation marks.
4. The zebrafish embryo culture device of claim 1, wherein the pore size of the filter membrane is 0.20-0.25 μm.
5. The zebrafish embryo culture device of claim 4, wherein the filter membrane has a pore size of 0.22 microns.
6. The zebrafish embryo culture device of claim 1, wherein the bottle body is made of glass.
7. The zebrafish embryo culture device of claim 1, wherein the bottle cap is made of plastic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010026609.1A CN111117887A (en) | 2020-01-10 | 2020-01-10 | Zebra fish embryo culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010026609.1A CN111117887A (en) | 2020-01-10 | 2020-01-10 | Zebra fish embryo culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111117887A true CN111117887A (en) | 2020-05-08 |
Family
ID=70487806
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010026609.1A Pending CN111117887A (en) | 2020-01-10 | 2020-01-10 | Zebra fish embryo culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111117887A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN206418144U (en) * | 2017-01-13 | 2017-08-18 | 北京华诺奥美医学检验所有限公司 | A kind of permeability type lymphocyte blake bottle |
| CN206462138U (en) * | 2017-02-08 | 2017-09-05 | 北京农业生物技术研究中心 | A kind of blake bottle for being easy to tissue culture to operate |
| CN207468646U (en) * | 2017-08-16 | 2018-06-08 | 苏州米迪生物技术有限公司 | High efficiency cell culture bottle |
| CN208869599U (en) * | 2017-12-27 | 2019-05-17 | 深圳赛动生物自动化有限公司 | Biological culture flask |
-
2020
- 2020-01-10 CN CN202010026609.1A patent/CN111117887A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN206418144U (en) * | 2017-01-13 | 2017-08-18 | 北京华诺奥美医学检验所有限公司 | A kind of permeability type lymphocyte blake bottle |
| CN206462138U (en) * | 2017-02-08 | 2017-09-05 | 北京农业生物技术研究中心 | A kind of blake bottle for being easy to tissue culture to operate |
| CN207468646U (en) * | 2017-08-16 | 2018-06-08 | 苏州米迪生物技术有限公司 | High efficiency cell culture bottle |
| CN208869599U (en) * | 2017-12-27 | 2019-05-17 | 深圳赛动生物自动化有限公司 | Biological culture flask |
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|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200508 |