CN111117883B - Biological intelligent cell dynamic culture system - Google Patents
Biological intelligent cell dynamic culture system Download PDFInfo
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- CN111117883B CN111117883B CN201910241675.8A CN201910241675A CN111117883B CN 111117883 B CN111117883 B CN 111117883B CN 201910241675 A CN201910241675 A CN 201910241675A CN 111117883 B CN111117883 B CN 111117883B
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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Abstract
The invention relates to a dynamic culture system of biological intelligent cells, which comprises a support frame (100) provided with a clamp clamping groove (101), wherein the upper edge of the clamp clamping groove (101) is clamped with the outer edge of a culture pond (102); a drive unit (200) which comprises a housing (201), a first motor (202), a turntable (203), a pin (204), a first link (205), a slide bar (206), a first guide rail (207), and a support bar (208); the clamp (300) comprises a first clamping head (301) and a second clamping head (302). The invention generates relative movement by a second clamping head (302) fixed with a support frame (100) and a first clamping head (301) moved by a driving part (200), thereby enabling a film (400) arranged on the first clamping head (301) and the second clamping head (302) to be regularly stretched and relaxed.
Description
Technical Field
The invention relates to an experimental tool, in particular to a system for stretching and culturing cells.
Background
Cells (English name: cell) are not uniformly defined, and the more common method is: cells are the basic structural and functional units of an organism. It is known that all organisms except viruses are composed of cells, but viral life activities must be expressed in cells.
In the conventional cell stretching experiment, if cells are required to be attached to a circular membrane (400), the circular membrane (400) is placed at the bottleneck of a variable-air-pressure bottle, and the concave-convex state of the membrane (400) is changed by adjusting the air pressure of the variable-air-pressure bottle during the experiment, so that the size of the cells attached to the membrane (400) is stretched. The cell stretching method is a method of stretching cells by equal ratio amplification, but some cells can be stretched only in one direction.
Therefore, there is a need for a multifunctional culture system capable of stretching cells in one direction.
Disclosure of Invention
The invention aims to provide a culture system capable of stretching cells in a single direction.
The invention relates to a biological intelligent cell dynamic culture system, which comprises
The support frame is provided with a clamp clamping groove, and the upper edge of the clamp clamping groove is clamped with the outer edge of the culture pond;
the driving part comprises a shell, a first motor, a rotary table, a pin rod, a first connecting rod, a sliding rod, a first guide rail and a support rod, wherein the shell is fixed with the support frame, the inner surface of the shell is fixed with the first motor, the output shaft of the first motor is connected with the rotary table, the surface of the rotary table, which is far away from the first motor, is provided with the pin rod through a bearing, the pin rod is fixed with the first connecting rod, the first connecting rod is hinged with one end of the sliding rod, the sliding rod transversely moves along the first guide rail, the first guide rail is fixed with the shell, one end of the shell, which is provided with a first through hole through which the sliding rod can extend out, and the other end of the sliding rod is fixed with the support rod;
and the fixture comprises a first chuck and a second chuck, one end of the first chuck is fixed with the supporting rod, and one end of the second chuck is fixed with one side, far away from the supporting rod, of the fixture clamping groove of the supporting frame.
The invention relates to a biological intelligent cell dynamic culture system, wherein a first clamping head, a second clamping head and two ends of a film are fixed, and the film is used for culturing cells.
The invention relates to a biological intelligent cell dynamic culture system, wherein a first clamping head and a second clamping head are fixed at two ends of animal tissues.
The invention relates to a biological intelligent cell dynamic culture system, wherein a support frame is provided with an extrusion part, the extrusion part comprises a first rod body, a spiral pipe, a top ball, a rope body, a magnet ball and a permanent magnet block, the lower end of the first rod body is fixed with the support frame, the upper end of the first rod body is fixed with the spiral tube, one end of the spiral pipe faces the end face of the first chuck, the other end of the spiral pipe faces the midpoint of a connecting line between the first chuck and the second chuck from top to bottom, a plurality of top balls are arranged in the spiral pipe along the axial direction of the spiral pipe, magnet balls with the same shape as the top balls are arranged in the middle of the top balls, a permanent magnet block is fixed in the middle of the spiral pipe and mutually attracted with the magnet ball, the plurality of top balls and the magnet balls are connected in series through the rope body and are fixed with each other, and the top ball at one end of the spiral pipe is in contact with the end face of the first chuck.
The invention relates to a biological intelligent cell dynamic culture system, wherein a rope body is a water pipe, one end of the rope body extends into the water pump from a top ball close to a first chuck and is connected with the water pump, at least four water spray holes are formed in the radial direction of the top ball close to a film, one water spray hole is communicated with the rope body, a culture solution is arranged in the water pump, the bottom of a culture pond is communicated with the upper end of a sewer pipe, the middle part of the sewer pipe penetrates through a support frame, and the upper part of the sewer pipe is provided with a valve.
The invention relates to a biological intelligent cell dynamic culture system, wherein a driving part is provided with a control module, the control module is connected with a power module of a first motor, and the control module comprises
A data input module for inputting the type of cells, the number b of cells and the culture time c;
a database provided with a type coefficient a corresponding to a cell type;
a frequency output module which outputs a type coefficient a corresponding to the type of the cell according to the database, and outputs a frequency P according to the type coefficient a, the number b of the cell, and the culture time c according to the following formula:
wherein the unit of the output frequency P is Hz;
the unit of the cell number b is hundreds, and the integral number is retained by the rounding method;
the unit of the culture time c is 6 hours, and when the culture time c is less than 6 hours, an integral number is reserved by adopting a further method;
the control module controls a power module of the first motor to drive the first chuck at the frequency P according to the output frequency P.
The invention relates to a biological intelligent cell dynamic culture system, wherein a driving part is provided with a control module, the control module is connected with a power module of a first motor, and the control module comprises
A data input module for inputting the type of cells, the number b of cells and the culture time c;
a database provided with a type coefficient a corresponding to a cell type;
a stretching width output module which outputs a type coefficient a corresponding to the type of the cell according to the database, and outputs a width F according to the type coefficient a, the number b of the cells and the culture time c according to the following formula:
wherein the unit of the output frequency F is millimeter;
the unit of the cell number b is hundreds, and the integral number is retained by the rounding method;
the unit of the culture time c is 6 hours, and when the culture time c is less than 6 hours, an integral number is reserved by adopting a further method;
and the control module controls the power module of the first motor to drive the motion amplitude of the first chuck by the amplitude F according to the output amplitude F.
The movement amplitude of the first chuck is the difference value between the longest extending distance and the shortest retracting distance.
The invention relates to a biological intelligent cell dynamic culture system, wherein a clamp is provided with a stirring part, the stirring part comprises a second connecting rod, a stirring blade, a clamping pin and a third connecting rod, one end of a second chuck is fixed with one end of the second connecting rod, the other end of the second connecting rod is hinged with one end of the stirring blade, the upper end surface of the stirring blade is provided with a long circular hole, the clamping pin moving along the long circular hole is arranged in the long circular hole, the clamping pin is fixed with one end of the third connecting rod, and the other end of the third connecting rod is hinged with one end of the first chuck.
The invention relates to a dynamic culture system of biological intelligent cells, which is characterized in that a second chuck fixed with a support frame and a first chuck moved by a driving part generate relative motion, so that a film arranged on the first chuck and the second chuck is regularly stretched and relaxed, and the motion is continuously repeated by the rotation of a first motor, so that the cells attached on the film are transversely stretched, thereby meeting the requirements of cell culture experiments.
The invention is further explained by the dynamic culture system of the biological intelligent cell in the following with the attached drawings.
Drawings
FIG. 1 is an isometric view of a dynamic culture system for biological intelligent cells;
FIG. 2 is an exploded view of a portion of a dynamic biological intelligence cell culture system;
FIG. 3 is a top view of a driving part of the dynamic biological intelligent cell culture system;
FIG. 4 is a partial top view of an extrusion portion of the dynamic biological intelligence cell culture system;
FIG. 5 is a partial front view of the extrusion portion of the dynamic biological intelligent cell culture system;
FIG. 6 is an enlarged view of a portion of FIG. 4;
FIG. 7 is a top view of a variation of the structure of FIG. 4;
fig. 8 is a partially enlarged view of fig. 7.
Detailed Description
As shown in FIGS. 1 to 8, referring to FIGS. 1 to 3, the system for dynamically culturing the bio-intelligent cells of the present invention comprises
The supporting frame 100 is provided with a clamp clamping groove 101, and the upper edge of the clamp clamping groove 101 is clamped with the outer edge of the culture pond 102;
a driving part 200, which includes a housing 201, a first motor 202, a rotating plate 203, a pin 204, a first link 205, a sliding rod 206, a first guide rail 207, and a support rod 208, wherein the housing 201 is fixed to the supporting frame 100, an inner surface of the housing 201 is fixed to the first motor 202, an output shaft of the first motor 202 is connected to the rotating plate 203, the pin 204 is mounted on a surface of the rotating plate 203, which is away from the first motor 202, through a bearing, the pin 204 is fixed to the first link 205, the first link 205 is hinged to one end of the sliding rod 206, the sliding rod 206 moves laterally along the first guide rail 207, the first guide rail 207 is fixed to the housing 201, one end of the sliding rod 206 of the housing 201 is provided with a first through hole through which the sliding rod can extend, and the other end of the sliding rod 206 is fixed to the support rod 208;
the clamp 300 comprises a first clamp 301 and a second clamp 302, wherein one end of the first clamp 301 is fixed to the support rod 208, and one end of the second clamp 302 is fixed to one side, far away from the support rod 208, of the clamp slot 101 of the support frame 100.
The present invention generates a relative movement between the second chuck 302 fixed to the support frame 100 and the first chuck 301 moved by the driving part 200, so that the thin film 400 disposed on the first chuck 301 and the second chuck 302 is regularly stretched and relaxed, and the above movement is repeated by the rotation of the first motor 202, so that the cells attached to the thin film 400 are stretched in the transverse direction to meet the requirements of the cell culture experiment.
Wherein the film 400 can be a PDMS film 400
The first clip 301 and the second clip 302 are disposed opposite to each other.
The two first links 205, the sliding bar 206, the first guide rail 207, and the support bar 208 may be arranged in a left-right symmetrical manner.
The left and right support rods 208 can be driven to move simultaneously by the symmetrically arranged parts, wherein the movement of the two support rods 208 is simultaneously leftward or simultaneously rightward.
The output shaft of the first motor 202 is provided with a first belt pulley 221, the first belt pulley 221 is connected with a conveyor belt 220, a first shaft 210 is fixed in the axial direction of the turntable 203, a second belt pulley 222 is coaxially fixed on the first shaft 210, and the second belt pulley 222 is connected with the first belt pulley 221 through the conveyor belt 220.
The invention reduces the longitudinal space of the shell by the first motor 202 and the rotary disc 203 which are transversely arranged through the conveying mode, thereby more fully utilizing the transverse space of the shell 201.
Wherein, nutrient solution is configured in the culture pond 102, and the nutrient solution can provide nutrients for the stretched cells.
The supporting frame 100 and the shell 201 are made of high-quality stainless steel or aluminum alloy, have good impact resistance, are easy to clean, and can be wiped by alcohol and sterilized by ultraviolet lamp irradiation.
Wherein the outer dimensions of the support frame 100 and the housing 201 are 120mm x 200mm x 73mm, the invention can be placed in a carbon dioxide incubator or workstation to culture cells and observe the cells.
The support frame 100 of the present invention may be configured with 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 fixture slots 101, and may be configured to hold fixtures 300 and culture wells 102 of different sizes.
In the present invention, the first motor 202 employs a precise motor control method to achieve precise mechanical stretching, and has better uniformity and higher precision compared with a gas pump method.
The power module of the first motor 202 is connected to the control module, and the control module is used for controlling the rotation speed, rotation direction, rotation angle, rotation time and reciprocating frequency of the first motor 202, so as to control the deformation parameters of cell stretching, thereby meeting the experimental requirements of different deformation quantities. The deformation parameters of the cell stretching may be: 5%, 10%, 15%, 20%, 25%, wherein the cell stretching deformation parameter is the ratio of the volume representing the amount of growth of the stretched cell to the volume of the original cell. Wherein the reciprocating frequency can be: 0.5Hz to 2Hz, wherein the reciprocating frequency is the number of times the first collet 301 reciprocates within one second.
The invention can be used for dynamically culturing cell samples, in-vitro tissue samples and biomedical material samples and is provided with clamps 300 with different specifications.
The invention can adopt PDMS films 400 with different specifications to dynamically culture cells for different cell samples, and different culture solutions are used for culturing in different culture ponds 102, thereby being convenient and quick without cross contamination risks.
In the invention, the isolated tissue and the biomedical material sample can be directly fixed and stretched by the clamp 300 without other consumables.
The invention can realize 24h uninterrupted operation, thereby continuing the experiment.
The invention can be directly placed under a microscope for observation, so that the operation is simple and convenient. .
The culture tank 102 of the support frame 100 of the present invention is provided with a temperature adjusting module that can adjust the temperature of the culture solution in the culture tank 102.
The culture tank 102 of the support frame 100 of the present invention is provided with an air conditioning module that can adjust the oxygen concentration and/or the carbon dioxide concentration of the culture solution of the culture tank 102.
The invention can be arranged in an operation box, and the operation box can be provided with an operation opening into which a hand can extend.
The operation box is provided with a humidity adjusting module which can adjust the humidity of the air in the operation box.
Each set of the first chuck 301, the second chuck 302, and the culture pond 102 is disposed in the same sealed bag, and both ends of the sealed bag are open, one end of the sealed bag is hermetically fixed to the first chuck 301, and the other end of the sealed bag is hermetically fixed to the second chuck 302, thereby controlling the temperature, humidity, oxygen concentration, and carbon dioxide concentration of the cells cultured in the sealed bag in a targeted manner.
Preferably, the first clip 301 and the second clip 302 are fixed to both ends of the thin film 400, and the thin film 400 is used for culturing cells.
In the present invention, the cell culture using the film 400 can be achieved by stretching the cell when the film 400 is bent, and then dynamically culturing the cell in combination with the nutrient solution in the culture tank 102.
Preferably, the first clip 301 and the second clip 302 are fixed to both ends of the animal tissue.
The cells in the animal tissue are stretched when the animal tissue is bent, and then the cells are matched with the nutrient solution in the culture pond 102 to realize dynamic culture.
Preferably, referring to fig. 4 and 5, the support frame 100 is provided with an extrusion portion 500, the extrusion portion 500 includes a first rod 501, a spiral pipe 502, a top ball 503, a rope 504, a magnet ball 505, and a permanent magnet 506, the lower end of the first rod 501 is fixed to the support frame 100, the upper end of the first rod 501 is fixed to the spiral pipe 502, one end of the spiral pipe 502 faces the end surface of the first chuck 301, the other end of the spiral pipe 502 faces the midpoint of the connecting line between the first chuck 301 and the second chuck 302 from top to bottom, the spiral pipe 502 is provided with a plurality of top balls 503 along the axial direction thereof, the middle of the plurality of top balls 503 is provided with the magnet ball 505 having the same shape as the top ball, the middle of the spiral pipe 502 is fixed with the permanent magnet 506, the permanent magnet 506 and the magnet ball 505 attract each other, the plurality of top balls 503 and the magnet ball 505 are connected in series through the rope 504 and fixed to each other, the top ball 503 at one end of the spiral pipe 502 contacts with the end face of the first chuck 301.
The invention can push the top ball 503 and the magnet ball 505 to reciprocate in the spiral pipe 502 by the first chuck 301 when the first chuck 301 reciprocates, and the spiral pipe 502 changes the transverse force of the first chuck 301 into the movement that the top ball 503 presses the film 400 longitudinally and downwards.
In use, the present invention may also place cells on the membrane 400 by:
s100, putting a culture solution into a culture pond 102;
s200, fixing two ends of a film 400 between a first clamping head 301 and a second clamping head 302;
s300, smearing cells to be cultured on the top ball 503 close to the film 400;
s400, flushing cells on the top ball 503 by a spray head with culture solution along the movement direction of the first chuck 301;
s400, the first motor 202 is started, so that the first chuck 301 is driven to reciprocate, and the cells on the top ball 503 are placed on the film 400 and are continuously pressed by the top ball 503 to assist the stretching of the cells.
The present invention can automatically place cells on the membrane 400 by the above method, and the cells on the membrane 400 are laterally washed by the nozzle to adjust to the posture to be stretched, for example, the cells all in a strip shape are laterally washed by the nozzle to adjust most of the cells to be laterally placed, and then placed on the membrane 400 by the top ball 503, so that most of the cells on the membrane 400 are stretched in the length direction of the cells. Of course, the present invention can also adjust the stretching direction of the cells as desired by spraying the culture solution in other directions.
The top ball 503 at one end of the spiral pipe 502 of the present invention may contact with the end surface of the first chuck 301, or may be fixed to the end surface of the first chuck 301. The top balls 503 and the magnet balls 505 can reciprocate in the solenoid 502, wherein the top balls 503 of the present invention reciprocate by means of attraction between the magnet balls 505 and the permanent magnet blocks 506 and pulling of the rope 504, even if the top ball 503 at one end of the solenoid 502 is not fixed but only contacts with the end surface of the first chuck 301. The floating advantage is that the first collet 301 can be removed and replaced at will without having to be removed along with the top ball 503 to facilitate cleaning of the present invention.
Preferably, referring to fig. 6, the rope 504 is a water pipe, one end of the rope 504 extends from the top ball 503 near the first chuck 301 and is connected to a water pump 601, at least four water spraying holes 602 are formed along the radial direction of the top ball 503 near the film 400, one of the water spraying holes is communicated with the rope 504, a culture solution is arranged in the water pump 601, the bottom of the culture tank 102 is communicated with the upper end of a downcomer 603, the middle of the downcomer 603 passes through the support frame 100, and the upper part of the downcomer 603 is provided with a valve.
The culture solution in the rope body 504 and the water pump 601 is sprayed out by taking the top ball 503 close to the film 400 as a spray head, and firstly, the culture solution is coated in the top ball 503 which extrudes cells; secondly, when the cells are placed, all the cells coated on the top ball 503 are washed to the film 400; further, when the culture medium is used for a long time and the culture medium needs to be replaced, the culture medium in the culture tank 102 can be replaced once in a manner that the culture medium is flushed with a new culture medium for a long time by discharging the culture medium through the heading ball 503 disposed at the upper portion of the thin film 400 and automatically repelling the waste culture medium through opening the valve at the lower water drain pipe 603 of the culture tank 102. In the invention, the culture solution is sprayed from top to bottom, so that the cells on the film 400 can be kept constantly, and the phenomenon of over-stem of the cells can not be caused.
The sewer pipe 603 is provided with a valve, which can be a wrench-type manual valve or an electronic valve, and any one of the valves in the prior art can be used, which is not described herein.
Preferably, the driving part 200 is provided with a control module, wherein the control module is connected with a power module of the first motor 202, and the control module comprises
A data input module for inputting the type of cells, the number b of cells and the culture time c;
a database provided with a type coefficient a corresponding to a cell type;
a frequency output module which outputs a type coefficient a corresponding to the type of the cell according to the database, and outputs a frequency P according to the type coefficient a, the number b of the cell, and the culture time c according to the following formula:
wherein the unit of the output frequency P is Hz;
the unit of the cell number b is hundreds, and the integral number is retained by the rounding method;
the unit of the culture time c is 6 hours, and when the culture time c is less than 6 hours, an integral number is reserved by adopting a further method;
the control module controls the power module of the first motor 202 to drive the first chuck 301 at the output frequency P.
According to the invention, the cells can be customized and cultured according to the specific conditions of the cells in the above way, namely, when the cell culture time is too long, the frequency is increased, so that the culture efficiency and the stretching efficiency are improved, and when the cells are too many, the frequency is reduced for facilitating the common stretching of the cells, so that the cells are prevented from being too dead.
Wherein, the cultivation time of the present invention is 6 hours, for example, when c is 0.333 and integer number is retained by the further method at the beginning of 2 hours of the present invention, c is 1 at (0, 6) hours and 2 at (6, 12) hours, wherein the cultivation time is about 1 to 2 days, that is, the definition domain of c is [4, 8 ].
Wherein, the output frequency P represents the number of times of controlling the power module of the first motor 202 to make the first chuck 301 reciprocate one turn in one second.
Wherein the number of cells b represents the number of cells cultured on the film 400, and the domain of b is [1, 20 ].
Wherein the database may include the following table:
preferably, the driving part 200 is provided with a control module, wherein the control module is connected with a power module of the first motor 202, and the control module comprises
A data input module for inputting the type of cells, the number b of cells and the culture time c;
a database provided with a type coefficient a corresponding to a cell type;
a stretching width output module which outputs a type coefficient a corresponding to the type of the cell according to the database, and outputs a width F according to the type coefficient a, the number b of the cells and the culture time c according to the following formula:
wherein the unit of the output frequency F is millimeter;
the unit of the cell number b is hundreds, and the integral number is retained by the rounding method;
the unit of the culture time c is 6 hours, and when the culture time c is less than 6 hours, an integral number is reserved by adopting a further method;
the control module controls the power module of the first motor 202 to drive the movement amplitude of the first chuck 301 with the amplitude F according to the output amplitude F.
Wherein, the movement amplitude of the first chuck 301 is the difference between the longest extending distance and the shortest retracting distance.
According to the invention, the cells can be subjected to customized culture according to the specific conditions of the cells, namely, the range is increased when the cell culture time is too long, so that the culture efficiency and the stretching efficiency are improved; when the number of cells is too large, the stretching efficiency is improved by increasing the width F for facilitating the co-stretching of the cells.
Preferably, referring to fig. 7 and 8, a stirring portion 700 is arranged on the clamp 300, the stirring portion 700 includes a second connecting rod 701, a stirring blade 702, a bayonet 704 and a third connecting rod 705, one end of the second chuck 302 is fixed to one end of the second connecting rod 701, the other end of the second connecting rod 701 is hinged to one end of the stirring blade 702, an oblong hole 703 is formed in the upper end surface of the stirring blade 702, the bayonet 704 moving along the oblong hole 703 is arranged in the oblong hole 703, the bayonet 704 is fixed to one end of the third connecting rod 705, and the other end of the third connecting rod 705 is hinged to one end of the first chuck 301.
According to the invention, the third connecting rod 705 is driven to transversely move by the reciprocating movement of the first clamping head 301, so that the stirring blade 702 can reciprocally swing towards the middle of the thin film 400, and the culture solution with higher nutrient concentration at the edge of the culture pond 102 flows towards the middle of the culture pond 102, thereby maintaining the uniformity of the culture solution in the culture pond 102 and facilitating the culture solution to continuously provide nutrition for the cells on the thin film 400.
In the present invention, the thin film 400 is bent every time the first chuck 301 moves when culturing cells, so that the cells are in contact with the culture solution in the culture well 102 when the thin film 400 is bent or the cells are in contact with the culture solution all the time, which causes the nutrient concentration of the culture solution in the culture well 102 to be non-uniform, and the stirring part 700 can ensure the uniformity of the culture solution, thereby culturing the cells more favorably.
The above-mentioned embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solution of the present invention by those skilled in the art should fall within the protection scope defined by the claims of the present invention without departing from the spirit of the present invention.
Claims (1)
1. A biological intelligent cell dynamic culture system is characterized in that: comprises that
The supporting frame (100) is provided with a clamp clamping groove (101), and the upper edge of the clamp clamping groove (101) is clamped with the outer edge of the culture pond (102);
the driving part (200) comprises a shell (201), a first motor (202), a rotary table (203), a pin rod (204), a first connecting rod (205), a sliding rod (206), a first guide rail (207) and a supporting rod (208), wherein the shell (201) is fixed with the supporting frame (100), the inner surface of the shell (201) is fixed with the first motor (202), an output shaft of the first motor (202) is connected with the rotary table (203), the pin rod (204) is installed on the surface, far away from the first motor (202), of the rotary table (203) through a bearing, the pin rod (204) is fixed with the first connecting rod (205), the first connecting rod (205) is hinged with one end of the sliding rod (206), the sliding rod (206) transversely moves along the first guide rail (207), the first guide rail (207) is fixed with the shell (201), and a first through hole capable of extending the sliding rod (206) is formed in one end of the shell (201), the other end of the sliding rod (206) is fixed with the supporting rod (208);
the clamp (300) comprises a first clamp head (301) and a second clamp head (302), one end of the first clamp head (301) is fixed with the supporting rod (208), and one end of the second clamp head (302) is fixed with one side, far away from the supporting rod (208), of the clamp clamping groove (101) of the supporting frame (100);
the first clamping head (301) and the second clamping head (302) are fixed with two ends of a thin film (400), and the thin film (400) is used for culturing cells;
the first clamping head (301) and the second clamping head (302) are fixed with two ends of animal tissues;
the support frame (100) is provided with an extrusion part (500), the extrusion part (500) comprises a first rod body (501), a spiral pipe (502), a top ball (503), a rope body (504), a magnet ball (505) and a permanent magnet block (506), the lower end of the first rod body (501) is fixed with the support frame (100), the upper end of the first rod body (501) is fixed with the spiral pipe (502), one end of the spiral pipe (502) faces the end face of the first chuck (301), the other end of the spiral pipe (502) faces the middle point of a connecting line between the first chuck (301) and the second chuck (302) from top to bottom, the spiral pipe (502) is internally provided with a plurality of top balls (503) along the axial direction thereof, the middles of the top balls (503) are provided with the magnet ball (505) with the same shape, the middle part of the spiral pipe (502) is fixed with the permanent magnet block (506), the permanent magnet blocks (506) and the magnet balls (505) are mutually attracted, the top balls (503) and the magnet balls (505) are connected in series through a rope body (504) and are mutually fixed, and the top ball (503) at one end of the spiral pipe (502) is in contact with the end face of the first chuck (301);
the rope body (504) is a water pipe, one end of the rope body (504) extends into the water pump (601) from a top ball (503) close to the first chuck (301) and is connected with the water pump (601), at least four water spray holes (602) are formed in the top ball (503) close to the film (400) along the radial direction of the top ball, one water spray hole (602) is communicated with the rope body (504), a culture solution is arranged in the water pump (601), the bottom of the culture pond (102) is communicated with the upper end of a sewer pipe (603), the middle of the sewer pipe (603) penetrates through the support frame (100), and a valve is arranged at the upper part of the sewer pipe (603);
the driving part (200) is provided with a control module, wherein the control module is connected with a power module of the first motor (202), and the control module comprises
A data input module for inputting the type of cells, the number b of cells and the culture time c;
a database provided with a type coefficient a corresponding to a cell type;
a frequency output module which outputs a type coefficient a corresponding to the type of the cell according to the database, and outputs a frequency P according to the type coefficient a, the number b of the cell, and the culture time c according to the following formula:
wherein the unit of the output frequency P is Hz;
the unit of the cell number b is hundreds, and the integral number is retained by the rounding method;
the unit of the culture time c is 6 hours, and when the culture time c is less than 6 hours, an integral number is reserved by adopting a further method;
the control module controls a power supply module of a first motor (202) to drive a first chuck (301) at the frequency P according to the output frequency P;
wherein, when the cell is classified as an organ cell, the type coefficient a is 10;
when the cell is classified as an epithelial cell or a myocyte, the type coefficient a is 8;
when the cells are classified as stem cells, the type coefficient a is 3;
the clamp is characterized in that a stirring part (700) is arranged on the clamp (300), the stirring part (700) comprises a second connecting rod (701), a stirring blade (702), a clamping pin (704) and a third connecting rod (705), one end of the second clamping head (302) is fixed with one end of the second connecting rod (701), the other end of the second connecting rod (701) is hinged with one end of the stirring blade (702), a long circular hole (703) is formed in the upper end face of the stirring blade (702), the clamping pin (704) moving along the long circular hole is arranged in the long circular hole (703), the clamping pin (704) is fixed with one end of the third connecting rod (705), and the other end of the third connecting rod (705) is hinged with one end of the first clamping head (301).
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1780266A1 (en) * | 2004-06-17 | 2007-05-02 | Ken Nakata | Cell culturing method by biomechanical stimulus load and its device |
| CN201908092U (en) * | 2010-11-08 | 2011-07-27 | 北京大学口腔医院 | Cell cyclic compression and tension device |
| CN103184144A (en) * | 2013-03-22 | 2013-07-03 | 中国科学院力学研究所 | Dynamic bidirectional-stretch in-situ online-observation cell biomechanics loading device |
| CN108130274A (en) * | 2017-08-18 | 2018-06-08 | 上海健康医学院 | A kind of cell culture apparatus and its method under pressure and single direction strain correlation |
| CN207918864U (en) * | 2017-10-16 | 2018-09-28 | 广州新诚生物科技有限公司 | The changing liquid automatically device of cell culture and the cell tensile device of the device |
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2019
- 2019-03-28 CN CN201910241675.8A patent/CN111117883B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1780266A1 (en) * | 2004-06-17 | 2007-05-02 | Ken Nakata | Cell culturing method by biomechanical stimulus load and its device |
| CN201908092U (en) * | 2010-11-08 | 2011-07-27 | 北京大学口腔医院 | Cell cyclic compression and tension device |
| CN103184144A (en) * | 2013-03-22 | 2013-07-03 | 中国科学院力学研究所 | Dynamic bidirectional-stretch in-situ online-observation cell biomechanics loading device |
| CN108130274A (en) * | 2017-08-18 | 2018-06-08 | 上海健康医学院 | A kind of cell culture apparatus and its method under pressure and single direction strain correlation |
| CN207918864U (en) * | 2017-10-16 | 2018-09-28 | 广州新诚生物科技有限公司 | The changing liquid automatically device of cell culture and the cell tensile device of the device |
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