CN111117874A - Culture medium preparation device with stirring and cooling functions - Google Patents
Culture medium preparation device with stirring and cooling functions Download PDFInfo
- Publication number
- CN111117874A CN111117874A CN202010112766.4A CN202010112766A CN111117874A CN 111117874 A CN111117874 A CN 111117874A CN 202010112766 A CN202010112766 A CN 202010112766A CN 111117874 A CN111117874 A CN 111117874A
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- cooling
- stirring
- culture medium
- clamping cavity
- medium preparation
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- 238000003756 stirring Methods 0.000 title claims abstract description 61
- 238000001816 cooling Methods 0.000 title claims abstract description 46
- 238000011177 media preparation Methods 0.000 title claims abstract description 27
- 239000001963 growth medium Substances 0.000 title claims abstract description 23
- 239000003814 drug Substances 0.000 claims abstract description 24
- 239000007788 liquid Substances 0.000 claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- 238000005485 electric heating Methods 0.000 claims abstract description 6
- 239000000498 cooling water Substances 0.000 claims description 27
- 239000003638 chemical reducing agent Substances 0.000 claims description 8
- 238000010438 heat treatment Methods 0.000 claims description 5
- 230000005540 biological transmission Effects 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 6
- 244000005700 microbiome Species 0.000 abstract description 2
- 230000001954 sterilising effect Effects 0.000 description 8
- 238000004659 sterilization and disinfection Methods 0.000 description 8
- 230000001276 controlling effect Effects 0.000 description 6
- 230000001105 regulatory effect Effects 0.000 description 4
- 238000009835 boiling Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000005213 imbibition Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M99/00—Subject matter not otherwise provided for in other groups of this subclass
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention belongs to the technical field of microorganism culture equipment, and particularly relates to a culture medium preparation device with stirring and cooling functions, which comprises an outer barrel, an inner barrel arranged in the outer barrel, a high-pressure cover body arranged at the top of the outer barrel and a plurality of movable wheels fixedly arranged on the lower end surface of the outer barrel, wherein a clamping cavity is arranged between the inner wall of the inner barrel and the inner wall of the outer barrel, water is filled in the clamping cavity, an electric heating pipe is arranged at the bottom of the clamping cavity, a spiral cooling coil pipe is arranged in the clamping cavity, and the cooling coil pipe is sleeved on the outer side of the inner barrel; install agitator motor on the high pressure lid, agitator motor's output is connected with the reduction gear, has overcome the not enough of prior art, through setting up cooling module, has promoted the cooling rate of liquid medicine, can also the temperature of accurate control liquid medicine, and the rabbling mechanism of tilting produces tangential rotation rivers and axial pressurized-water vortex in addition for the liquid medicine constantly rolls in turn inside and outside, reaches the effect of intensive mixing.
Description
Technical Field
The invention belongs to the technical field of microorganism culture equipment, and particularly relates to a culture medium preparation device with stirring and cooling functions.
Background
The principle of the medium preparation apparatus currently used in the market is simple because the boiling point of water is affected by the air pressure, and the higher the air pressure, the higher the boiling point. On high mountains and plateaus, the air pressure is less than 1 atmosphere, and water can be boiled at the temperature of less than 100 ℃. At pressures above 1 atmosphere, the water will boil at temperatures above 100 ℃. The medium preparation device commonly used by people is designed by utilizing the principle. The culture medium preparation device seals the water tightly, the water vapor generated by the evaporation of the water after heating can not be diffused into the air and can only be kept in the culture medium preparation device, so that the air pressure in the culture medium preparation device is higher than 1 atmospheric pressure, and the water is boiled when the temperature is higher than 100 ℃, thus the high-temperature and high-pressure environment is formed in the culture medium preparation device. The existing culture medium preparation device generates a high-temperature and high-pressure environment in a closed container, and the liquid medicine is sterilized within a certain temperature and pressure limit range.
The existing culture medium preparation device has the following two defects in the using process: firstly, because the liquid medicine inlayer and the skin in the container are heated inhomogeneously when heating, central zone temperature can not reach sterilization temperature, can not thoroughly sterilize, and the sterilization has the dead angle. Secondly, after the sterilization is finished, the liquid medicine needs to be cooled to a certain low temperature for use, the time of a natural cooling mode is long, the waiting time is too long, and the working efficiency is low.
Disclosure of Invention
The invention aims to provide a culture medium preparation device with stirring and cooling functions, which overcomes the defects of the prior art, improves the cooling speed of liquid medicine by arranging a cooling assembly, can also accurately control the temperature of the liquid medicine, and in addition, an inclined stirring mechanism generates tangential rotating water flow and axial pressurized water vortex flow, so that the liquid medicine is continuously and alternately rolled inside and outside, and the effect of fully and uniformly stirring is achieved.
In order to solve the problems, the technical scheme adopted by the invention is as follows:
a culture medium preparation device with stirring and cooling functions comprises an outer barrel, an inner barrel arranged in the outer barrel, a high-pressure cover arranged at the top of the outer barrel and a plurality of movable wheels fixedly arranged on the lower end face of the outer barrel, wherein a clamping cavity is formed between the inner walls of the inner barrel and the outer barrel, water is filled in the clamping cavity, an electric heating pipe is arranged at the bottom of the clamping cavity, a spiral cooling coil is arranged in the clamping cavity, and the cooling coil is sleeved on the outer side of the inner barrel; the stirring device comprises a high-pressure cover body, a stirring motor, a speed reducer, a stirring paddle and a stirring blade, wherein the high-pressure cover body is provided with the stirring motor, the output end of the stirring motor is connected with the speed reducer, an output shaft of the stirring motor penetrates through the high-pressure cover body through the speed reducer and extends to the middle section of the inner part of the inner barrel, and the outer side of the bottom end of the; the top end of the stirring motor is provided with a speed regulation switch for controlling the opening and closing of the stirring motor and the rotating speed of the stirring motor, and one side of the outer barrel is provided with a control box for controlling the heating power of the electric heater and the flow of cooling water in the cooling coil.
Further, the upper end and the lower end of the cooling coil are respectively provided with a cooling water outlet and a cooling water inlet, the cooling water outlet and the cooling water inlet penetrate through the outer barrel and extend to the outer barrel, flow control valves are arranged on the cooling water outlet and the cooling water inlet, and the flow control valves are connected with the control box through wires.
Furthermore, a plurality of guide rods in the vertical direction are fixedly arranged in the clamping cavity, and the cooling coil is fixed on the guide rods.
Furthermore, the center of the upper end face of the high-pressure cover body is connected with a screw rod through a shaft sleeve thread, and a hand wheel is fixedly arranged at the top end of the screw rod.
Furthermore, the stirring motor is positioned on one side of the hand wheel, and an output shaft of the stirring motor is obliquely inserted into the inner cylinder.
Further, install temperature sensor and pipette on the high pressure lid, temperature sensor is used for detecting the temperature of the inside liquid medicine of inner tube to give the control box with it, the pipette runs through the high pressure lid, and its bottom extends to the inside bottom of inner tube.
Compared with the prior art, the invention has the following beneficial effects:
according to the culture medium preparation device with the stirring and cooling functions, the cooling speed of the liquid medicine is increased by arranging the cooling assembly and the temperature sensor, the temperature of the liquid medicine can be accurately controlled, and the inclined stirring mechanism is arranged to generate tangential rotating water flow and axial pressurized water vortex flow, so that the liquid medicine is continuously and alternately rolled inside and outside, and the effect of fully and uniformly stirring is achieved; through setting up the pipette, avoid the manual work to open the imbibition of high pressure lid, avoid producing secondary pollution.
The sterilization device has the advantages of simple structure, convenience in operation and strong practicability, enables the liquid medicine to be uniformly heated, thoroughly sterilizes, accelerates the cooling of the liquid medicine, and improves the sterilization efficiency.
Drawings
FIG. 1 is a schematic structural diagram of a medium preparation device with stirring and cooling functions.
FIG. 2 is a front view of a medium preparing apparatus with stirring and cooling functions.
FIG. 3 is a plan view of a medium preparation apparatus with stirring and cooling functions.
In the figure: 1. an outer tub; 2. an inner barrel; 3. a cooling coil; 4. a control box; 5. a guide bar; 6. a high-pressure cover body; 7. a temperature sensor; 8. a pipette; 9. a hand wheel; 10. a screw rod; 11. a stirring motor; 12. a speed regulating switch; 13. a speed reducer; 14. a cooling water outlet; 15. a stirring paddle; 16. a clamp cavity; 17. a cooling water inlet; 18. an electric heating tube; 19. the wheel is moved.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1-3, the culture medium preparation device with stirring and cooling functions according to the present invention comprises an outer barrel 1, an inner barrel 2 disposed inside the outer barrel 1, a high pressure cover 6 disposed on the top of the outer barrel 1, and a plurality of moving wheels 19 fixedly disposed on the lower end surface of the outer barrel 1, wherein a clamping cavity 16 is disposed between the inner walls of the inner barrel 2 and the outer barrel 1, the clamping cavity 16 is filled with water, an electric heating tube 18 is disposed at the bottom of the clamping cavity 16, a spiral cooling coil 3 is disposed in the clamping cavity 16, and the cooling coil 3 is sleeved on the outer side of the inner barrel 2; the stirring motor 11 is installed on the high-pressure cover body 6, the output end of the stirring motor 11 is connected with the speed reducer 13, the output shaft of the stirring motor 11 penetrates through the high-pressure cover body 6 through the speed reducer 13 and extends to the middle section inside the inner barrel 2, and the outer side of the bottom end of the output shaft of the stirring motor 11 is fixedly provided with the stirring paddle 15; the top end of the stirring motor 11 is provided with a speed regulating switch 12 for controlling the opening and closing of the stirring motor 11 and the rotating speed, and one side of the outer barrel 1 is provided with a control box 4 for controlling the heating power of the electric heater and the flow of cooling water in the cooling coil 3.
The upper end and the lower end of the cooling coil 3 are respectively a cooling water outlet 14 and a cooling water inlet 17, the cooling water outlet 14 and the cooling water inlet 17 all penetrate through the outer barrel 1 and extend to the outside of the outer barrel 1, flow control valves are arranged on the cooling water outlet 14 and the cooling water inlet 17 and connected with the control box 4 through wires, and the flow of the cooling water can be controlled to finely control the temperature of the liquid medicine.
A plurality of guide rods 5 in the vertical direction are fixedly arranged in the clamping cavity 16, and the cooling coil 3 is fixed on the guide rods 5.
The center of the upper end face of the high-pressure cover body 6 is connected with a screw rod 10 through shaft sleeve threads, the top end of the screw rod 10 is fixedly provided with a hand wheel 9, and the high-pressure cover body 6 is matched with the screw rod 10 and the hand wheel 9 to improve the sealing performance.
The stirring motor 11 is positioned at one side of the hand wheel 9, and an output shaft of the stirring motor 11 is obliquely inserted into the inner barrel 2.
Install temperature sensor 7 and pipette 8 on the high pressure lid 6, temperature sensor 7 is used for detecting the temperature of the inside liquid medicine of inner tube 2 to give control box 4 with it transmission, pipette 8 runs through high pressure lid 6, and its bottom extends to the inside bottom of inner tube 2, and pipette 8 effect is the liquid medicine of bucket in the extraction, does not need the manual work to open the lid, prevents that artifical contact liquid medicine and entering air from producing secondary pollution.
In conclusion, when the culture medium preparation device with stirring and cooling functions is used, a small amount of water is injected into the clamping cavity 16, a liquid medicine is added into the inner cylinder 2, the high-pressure cover body 6 is covered, the hand wheel 9 is screwed down to form a sealed space between the high-pressure cover body 6 and the clamping cavity 16, the water at the bottom of the clamping cavity 16 is heated by the electric heating pipe 18 arranged at the bottom of the clamping cavity 16 to generate high-temperature steam, and because the steam in the closed container cannot leak out, the steam pressure in the culture medium preparation device is gradually increased, and the water is boiled when the temperature is higher than 100 ℃ after the pressure is higher than 1 atmosphere, so that a high-temperature and high-pressure environment is formed in the culture medium preparation device to sterilize the liquid medicine; in the sterilization process, the stirring motor 11 is turned on through the speed regulating switch 12, the rotating speed is set, the stirring motor 11 drives the stirring paddle 15 to rotate, the temperature and the pressure are raised, the stirring is carried out while the temperature and the pressure are raised, the sterilization dead angle is avoided, the thorough sterilization is realized, the speed regulating switch 12 is used for controlling the stirring speed of the motor, the inclined stirring paddle 15 generates tangential rotating water flow and axial pressurized water vortex, the liquid medicine is enabled to roll inside and outside alternately continuously, and the effect of fully and uniformly stirring is achieved; after the set time is reached, the control box 4 opens the flow control valves of the cooling water inlet 17 and the cooling water outlet 14, then fills cold water, and carries out water cooling through the cooling coil 3; the control box 4 monitors the temperature, and slows down the flow rate of the cooling water when the temperature is close to the set temperature, thereby achieving the effect of finely controlling the temperature of the liquid medicine.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (6)
1. The utility model provides a culture medium preparation ware of area stirring and cooling function, includes outer bucket (1), sets up at inner tube (2) of outer bucket (1) inside, sets up high-pressure lid (6) at outer bucket (1) top and sets firmly a plurality of wheels (19) that remove of terminal surface under outer bucket (1), its characterized in that: a clamping cavity (16) is arranged between the inner wall of the inner barrel (2) and the inner wall of the outer barrel (1), water is filled in the clamping cavity (16), an electric heating pipe (18) is installed at the bottom of the clamping cavity (16), a spiral cooling coil (3) is arranged in the clamping cavity (16), and the cooling coil (3) is sleeved on the outer side of the inner barrel (2); a stirring motor (11) is installed on the high-pressure cover body (6), the output end of the stirring motor (11) is connected with a speed reducer (13), the output shaft of the stirring motor (11) penetrates through the high-pressure cover body (6) through the speed reducer (13) and extends to the inner middle section of the inner barrel (2), and a stirring paddle (15) is fixedly arranged on the outer side of the bottom end of the output shaft of the stirring motor (11); the speed regulation switch (12) of controlling the stirring motor (11) to open and close and the rotational speed is installed on the top of stirring motor (11), control box (4) is installed to outer bucket (1) one side for the heating power of control electric heater and the flow of cooling water in cooling coil (3).
2. The culture medium preparation device with stirring and cooling functions as claimed in claim 1, wherein: the upper end and the lower end of the cooling coil (3) are respectively provided with a cooling water outlet (14) and a cooling water inlet (17), the cooling water outlet (14) and the cooling water inlet (17) all penetrate through the outer barrel (1) and extend to the outer barrel (1), flow control valves are arranged on the cooling water outlet (14) and the cooling water inlet (17), and the flow control valves are connected with the control box (4) through wires.
3. The culture medium preparation device with stirring and cooling functions as claimed in claim 2, wherein: and a plurality of guide rods (5) in the vertical direction are fixedly arranged in the clamping cavity (16), and the cooling coil (3) is fixed on the guide rods (5).
4. The culture medium preparation device with stirring and cooling functions as claimed in claim 1, wherein: the center of the upper end face of the high-pressure cover body (6) is connected with a screw rod (10) through shaft sleeve threads, and a hand wheel (9) is fixedly arranged at the top end of the screw rod (10).
5. The culture medium preparation device with stirring and cooling functions as claimed in claim 4, wherein: the stirring motor (11) is positioned on one side of the hand wheel (9), and an output shaft of the stirring motor (11) is obliquely inserted into the inner barrel (2).
6. The culture medium preparation device with stirring and cooling functions as claimed in claim 1 or 4, wherein: install temperature sensor (7) and pipette (8) on high pressure lid (6), temperature sensor (7) are used for detecting the temperature of the inside liquid medicine of inner tube (2) to give control box (4) with it transmission, pipette (8) run through high pressure lid (6), and its bottom extends to the inside bottom of inner tube (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010112766.4A CN111117874A (en) | 2020-02-24 | 2020-02-24 | Culture medium preparation device with stirring and cooling functions |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010112766.4A CN111117874A (en) | 2020-02-24 | 2020-02-24 | Culture medium preparation device with stirring and cooling functions |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111117874A true CN111117874A (en) | 2020-05-08 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010112766.4A Pending CN111117874A (en) | 2020-02-24 | 2020-02-24 | Culture medium preparation device with stirring and cooling functions |
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| Country | Link |
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| CN (1) | CN111117874A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1331662A (en) * | 1970-09-25 | 1973-09-26 | Netzsch Maschinenfabrik | Agitator mill |
| JP2013128470A (en) * | 2011-12-22 | 2013-07-04 | Toray Ind Inc | Method for sterilizing separation membrane module, method for producing chemicals, device for sterilizing separation membrane module, and membrane separation type continuous fermentation device |
| JP2016136942A (en) * | 2015-01-21 | 2016-08-04 | 株式会社高井製作所 | Heating method and heating device of mashed-soybean soup |
| CN105983120A (en) * | 2015-01-30 | 2016-10-05 | 广州市迪景微生物科技有限公司 | Sterilizing pot |
| CN209361379U (en) * | 2018-11-29 | 2019-09-10 | 天津海奥科技有限公司 | Disinfection tank is used in a kind of production of shampoo |
| CN209437699U (en) * | 2018-12-19 | 2019-09-27 | 河北冈大生物科技有限公司 | A kind of high-pressure sterilizing pot for enriched medium production |
| CN211734365U (en) * | 2020-02-24 | 2020-10-23 | 合肥天达诊断试剂有限公司 | Culture medium preparation device with stirring and cooling functions |
-
2020
- 2020-02-24 CN CN202010112766.4A patent/CN111117874A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1331662A (en) * | 1970-09-25 | 1973-09-26 | Netzsch Maschinenfabrik | Agitator mill |
| JP2013128470A (en) * | 2011-12-22 | 2013-07-04 | Toray Ind Inc | Method for sterilizing separation membrane module, method for producing chemicals, device for sterilizing separation membrane module, and membrane separation type continuous fermentation device |
| JP2016136942A (en) * | 2015-01-21 | 2016-08-04 | 株式会社高井製作所 | Heating method and heating device of mashed-soybean soup |
| CN105983120A (en) * | 2015-01-30 | 2016-10-05 | 广州市迪景微生物科技有限公司 | Sterilizing pot |
| CN209361379U (en) * | 2018-11-29 | 2019-09-10 | 天津海奥科技有限公司 | Disinfection tank is used in a kind of production of shampoo |
| CN209437699U (en) * | 2018-12-19 | 2019-09-27 | 河北冈大生物科技有限公司 | A kind of high-pressure sterilizing pot for enriched medium production |
| CN211734365U (en) * | 2020-02-24 | 2020-10-23 | 合肥天达诊断试剂有限公司 | Culture medium preparation device with stirring and cooling functions |
Non-Patent Citations (1)
| Title |
|---|
| 陈静;董家新;梁士楚;赵丰丽;: "小型培养基制备与分装装置的研制", 实验科学与技术, no. 06, 28 December 2016 (2016-12-28), pages 85 - 86 * |
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