CN111088246A - Biochar immobilized naphthalene degrading microbial inoculum and preparation method and application thereof - Google Patents
Biochar immobilized naphthalene degrading microbial inoculum and preparation method and application thereof Download PDFInfo
- Publication number
- CN111088246A CN111088246A CN201911388327.XA CN201911388327A CN111088246A CN 111088246 A CN111088246 A CN 111088246A CN 201911388327 A CN201911388327 A CN 201911388327A CN 111088246 A CN111088246 A CN 111088246A
- Authority
- CN
- China
- Prior art keywords
- biochar
- naphthalene
- immobilized
- microbial inoculum
- reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
- C09K17/16—Soil-conditioning materials or soil-stabilising materials containing organic compounds only applied in a physical form other than a solution or a grout, e.g. as platelets or granules
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Soil Sciences (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Materials Engineering (AREA)
- Inorganic Chemistry (AREA)
- Mycology (AREA)
- Environmental & Geological Engineering (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a preparation method of a biochar immobilized naphthalene degrading microbial inoculum, which comprises the following steps: weighing biomass raw materials, mixing with water, heating for reaction, cooling at room temperature after the reaction is finished, carrying out solid-liquid separation, cleaning a solid product, and carrying out freeze drying and grinding to obtain biochar; mixing the naphthalene degrading strain and the biochar in an LB liquid culture medium, and placing the mixture in a constant-temperature shaking table for shaking culture to obtain uniform mixed bacterial liquid; and mixing the mixed bacterial liquid with a sodium alginate solution, obtaining the sodium alginate mixed bacterial liquid after magnetic stirring is uniform, dropwise adding the sodium alginate mixed bacterial liquid into a calcium chloride solution, standing, placing into a refrigerator for crosslinking reaction, and obtaining the biochar immobilized naphthalene degrading microbial inoculum after the reaction is finished. The biochar immobilized naphthalene degrading microbial inoculum prepared by the invention has an obvious effect of repairing polycyclic aromatic hydrocarbon polluted soil, is mild in reaction, low in cost, free of secondary pollution and wide in engineering application prospect.
Description
Technical Field
The invention relates to a charcoal immobilized naphthalene degrading microbial inoculum and a preparation method and application thereof, belonging to the technical field of microbial remediation of polluted soil.
Background
Naphthalene is a polycyclic aromatic hydrocarbon substance with a 2-ring structure, low toxicity and high efficiency, and is widely applied to the production of phthalic anhydride, dye intermediates, rubber additives, pesticides and the like. In 10 months in 2017, the international cancer research institution of the world health organization lists naphthalene in a 2B category carcinogen list, and then in 12 months in 2017, China lists the naphthalene in a first group of priority control chemical directory. At present, the remediation technology for polycyclic aromatic hydrocarbon-polluted soil mainly comprises heat treatment, chemical oxidation, chemical leaching and the like, and although the methods have obvious effect on removing polycyclic aromatic hydrocarbon, the environmental problems such as secondary pollution and the like are easily caused. In contrast, the microbial remediation technology has the advantages of economy, high efficiency, environmental friendliness and the like, and is an important process for effectively converting and removing polycyclic aromatic hydrocarbon substances in the polluted soil. Therefore, obtaining the functional microorganism with the high-efficiency degradation capability of the polycyclic aromatic hydrocarbon is the precondition and the key for realizing the success of the microbial remediation of the polluted soil.
In the early work, the inventor obtains a naphthalene high-efficiency degrading strain from petroleum hydrocarbon contaminated soil through screening, and the strain is identified as Paenibacillus napus (Paenibacillus napus) through 16S rDNA sequencing and homology ratio peer-to-peer analysis, and the GenBank accession number is SRR 10341461. Physical and chemical properties and degradation characteristics are analyzed, and the strain can grow and reproduce by taking naphthalene as a unique carbon source, has good metabolic capability on naphthalene, and can achieve a naphthalene degradation removal rate of 83.6 percent within 5 days under the condition of a culture medium. Considering the problems that free strains are easy to run off in practical application and are difficult to compete with soil indigenous microorganisms, the research and development of a proper immobilization technology is a key for ensuring that functional microorganisms can efficiently degrade pollutants. After the functional strains with good degradation capability obtained by screening are mixed with the immobilized carrier, the functional strains can be attached and grown in the immobilized carrier and on the surface of the immobilized carrier, and in practical application, on one hand, the density of the degrading strains in the polluted soil can be enriched, the adverse effect of the external environment and soil indigenous microorganisms on the functional strains can be weakened to a certain extent, and the degradation and removal effect of the functional strains on target pollutants is enhanced; on the other hand, the immobilized carrier can promote the directional migration of the pollutants to a certain extent, strengthen the interface contact between the functional strains and the pollutants, and improve the degradation and removal effect of the pollutants.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: provides a naphthalene degrading bacteria immobilization method and is used for combined remediation of polycyclic aromatic hydrocarbon contaminated soil.
In order to solve the technical problems, the invention provides a preparation method of a biochar immobilized naphthalene degrading microbial inoculum, which is characterized by comprising the following steps:
step 1): weighing a biomass raw material, mixing the biomass raw material with water in a polytetrafluoroethylene lining tank, putting the mixture into a stainless steel reaction kettle, heating for reaction, cooling at room temperature after the reaction is finished, carrying out solid-liquid separation, cleaning a solid product by using distilled water and absolute ethyl alcohol, and carrying out freeze drying and grinding to obtain biochar;
step 2): mixing the naphthalene degrading strain subjected to freezing preservation and the biochar obtained in the step 1) in an LB liquid culture medium, and placing the mixture in a constant-temperature shaking table for shaking culture to obtain uniform mixed bacterial liquid;
step 3): mixing the mixed bacterial liquid obtained in the step 2) with a sodium alginate solution, obtaining the sodium alginate mixed bacterial liquid after magnetic stirring is uniform, dropwise adding the sodium alginate mixed bacterial liquid into a calcium chloride solution, standing, placing in a refrigerator at 4 ℃ for crosslinking reaction, and obtaining the biochar immobilized naphthalene degrading microbial inoculum after the reaction is finished.
Preferably, the biomass raw material in the step 1) is lignin, cellulose or hemicellulose.
Preferably, the mass ratio of the biomass raw material to the water in the step 1) is 1 (10-30).
Preferably, the heating temperature in the step 1) is 220-280 ℃, and the heating reaction time is 2-4 h.
Preferably, the freeze-drying time in the step 1) is 12-24 h.
Preferably, the naphthalene degrading strain in the step 2) is Paenibacillus napus, the GenBank accession number is SRR10341461, and naphthalene can be used as a unique carbon source for growth and propagation.
Preferably, the mass ratio of the naphthalene degrading strain, the biochar and the LB liquid culture medium in the step 2) is 1 (20-50) to 250.
Preferably, the temperature of the constant temperature shaking table in the step 2) is controlled to be 37 ℃, the oscillation rate is 100-150 r/min, and the oscillation time is 12-24 h.
Preferably, the volume ratio of the mixed bacterial liquid to the sodium alginate solution in the step 3) is 1:1, the mass concentration of the sodium alginate solution is 2% -4%, the mass concentration of the calcium chloride solution is 2% -5%, and the volume ratio of the mixed bacterial liquid to the calcium chloride is 1: 2-4.
Preferably, the crosslinking reaction time in the step 3) is 12-24 h.
The invention also provides the charcoal immobilized naphthalene degrading microbial inoculum prepared by the method.
The invention also provides application of the biochar immobilized naphthalene degrading microbial inoculum in remediation of soil polluted by polycyclic aromatic hydrocarbons.
Preferably, the application comprises: fully mixing the biochar immobilized naphthalene degrading microbial inoculum with the polycyclic aromatic hydrocarbon polluted soil, simultaneously adding an inorganic salt solution, uniformly stirring, and sampling every 12 hours to determine the concentration of naphthalene residue in the polluted soil.
More preferably, in the application, the mass ratio of the biochar immobilized naphthalene degrading microbial inoculum to the soil polluted by the polycyclic aromatic hydrocarbon is 2-3: 1000, and the mass ratio of the inorganic salt solution to the soil polluted by the polycyclic aromatic hydrocarbon is 8-15: 100.
Further, the content of the components of the inorganic salt solution is 1g/L Na2HPO4、0.75g/L KH2PO4、0.5g/LNH4Cl、0.1g/L MgSO4·7H2O、0.01g/L CaCl2、0.5g/L NaNO3、0.03g/L FeSO4·7H2O and 0.005L/L trace element solution with pH value of 7.0-7.2, wherein the content of the trace element solution is 5g/L CuCl2·2H2O、1g/L ZnCl2、0.5g/L CoCl2·6H2O and 0.06g/L H3BO3。
Compared with the prior art, the invention has the following beneficial effects:
(1) the method of the invention fixes the naphthalene degrading strain in the charcoal carrier, on one hand, the charcoal can provide stable growth environment for the naphthalene degrading strain, and on the other hand, the environmental concentration of the naphthalene degrading strain can be improved. In addition, the biochar has a certain specific surface area, and can directionally adsorb polycyclic aromatic hydrocarbon substances in the polluted soil on the surface, enhance the interface contact and microbial reaction of the biochar and a naphthalene degradation strain, and realize the combined remediation of the polluted soil. The biochar is a good soil repairing and improving material, does not produce harmful influence on soil microorganisms, and has the advantages of being green, sustainable and the like.
(2) The method utilizes the biochar immobilized naphthalene degrading microbial inoculum and adopts sodium alginate for embedding, so that the method can be applied to remediation of the soil polluted by the polycyclic aromatic hydrocarbon, can obtain a better remediation effect, has the beneficial effects of mild reaction, low cost, no secondary pollution and the like compared with remediation methods such as chemical oxidation, heat treatment and the like, and has a wide engineering application prospect.
Drawings
FIG. 1 is a scanning electron microscope of the MCC280, a biochar material prepared in example 1 of the invention;
FIG. 2 is a diagram of an immobilized naphthalene degrading microbial inoculum of a biochar material MCC280 prepared in example 1 of the invention;
FIG. 3 is a scanning electron microscope of the MCC220 of the biochar material prepared in example 2 of the invention;
FIG. 4 is a diagram of the immobilized naphthalene degrading microbial inoculum of the biochar material MCC220 prepared in example 2 of the invention.
Detailed Description
The invention will be further illustrated with reference to the following specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Further, it should be understood that various changes or modifications of the present invention may be made by those skilled in the art after reading the teaching of the present invention, and such equivalents may fall within the scope of the present invention as defined in the appended claims.
The reagents used in the present invention: microcrystalline cellulose, disodium hydrogen phosphate, potassium dihydrogen phosphate, ammonium chloride, magnesium sulfate heptahydrate, calcium chloride, sodium nitrate, ferrous sulfate heptahydrate, copper chloride dihydrate, zinc chloride, cobalt chloride hexahydrate and boric acid are analytically pure and purchased from national pharmaceutical group chemical reagent (Shanghai) Co., Ltd; dichloromethane, n-hexane, and acetonitrile were all chromatographically pure and purchased from sigma aldrich trade ltd.
The naphthalene degrading strain is Paenibacillus naphthaphilus (Paenibacillus naphthalins) obtained by screening from petroleum hydrocarbon polluted soil by the inventor, and the GenBank accession number is SRR 10341461.
The detection method of the naphthalene degradation removal rate in the soil comprises the following steps: the determination is carried out according to the national standard of high performance liquid chromatography for determination of polycyclic aromatic hydrocarbons of soil and sediments (HJ 784-2016).
Example 1
The embodiment provides a preparation method of a biochar immobilized naphthalene degrading microbial inoculum, which comprises the following specific steps:
step 1: weighing 2g of microcrystalline cellulose, mixing the microcrystalline cellulose with water in a polytetrafluoroethylene lining tank, putting the mixture into a stainless steel reaction kettle, heating the mixture to 280 ℃ for reaction for 2 hours, cooling the mixture at room temperature after the reaction is finished, carrying out solid-liquid separation at the rotating speed of 12000r/min, repeatedly cleaning a solid product by using distilled water and absolute ethyl alcohol, carrying out freeze drying for 12 hours, and grinding to obtain the biochar named MCC280 (shown in figure 1);
step 2: mixing 0.1g of the naphthalene degradation strain subjected to freezing preservation and 2g of MCC280 biochar in 100mL of LB liquid medium, placing the mixture in a constant-temperature shaking table for shake culture for 12 hours at the temperature of 37 ℃ and the shaking rate of 150r/min to obtain uniform mixed bacterial liquid;
and step 3: mixing the mixed bacterial liquid with a sodium alginate solution according to a volume ratio of 1:1, wherein the mass concentration of the sodium alginate solution is 4%, magnetically stirring uniformly to obtain the sodium alginate mixed bacterial liquid, dropwise adding the sodium alginate mixed bacterial liquid into a calcium chloride solution with the mass concentration of 2% by using a 1mL needle tube, wherein the volume ratio of the sodium alginate mixed bacterial liquid to the calcium chloride solution is 1:2, standing, placing in a 4 ℃ refrigerator for a crosslinking reaction for 12 hours, and obtaining a biological carbon-280 MCC (see figure 2) degradation microbial agent after the reaction is finished.
Fully mixing the biological carbon MCC280 immobilized naphthalene degrading microbial inoculum (3g) and polycyclic aromatic hydrocarbon polluted soil (1kg) according to the mass ratio of 3:1000, wherein the mass concentration of naphthalene in the polluted soil is 50mg/L, adding an inorganic salt solution (150mL), uniformly stirring, and then placing in a constant-temperature shaking table at 37 ℃ for shaking culture for 5 days at the shaking speed of 150 r/min. Through determination, the naphthalene degradation removal rate in the polluted soil can reach 90.3%.
Example 2
The embodiment provides a preparation method of a biochar immobilized naphthalene degrading microbial inoculum, which comprises the following specific steps:
step 1: weighing 5g of microcrystalline cellulose, mixing the microcrystalline cellulose with water in a polytetrafluoroethylene lining tank, putting the mixture into a stainless steel reaction kettle, heating the mixture to 220 ℃ for reaction for 2 hours, cooling the mixture at room temperature after the reaction is finished, carrying out solid-liquid separation at the rotating speed of 12000r/min, repeatedly cleaning a solid product by using distilled water and absolute ethyl alcohol, carrying out freeze drying for 12 hours, and grinding to obtain the biochar named MCC220 (shown in figure 3);
step 2: mixing 0.1g of the naphthalene degradation strain subjected to freezing preservation and 5g of MCC220 biochar in 100mL of LB liquid medium, placing the mixture in a constant-temperature shaking table for shake culture for 12 hours at the temperature of 37 ℃ and the shaking rate of 150r/min to obtain uniform mixed bacterial liquid;
and step 3: and mixing the mixed bacterial liquid with a sodium alginate solution according to a volume ratio of 1:1, wherein the mass concentration of the sodium alginate solution is 2%, magnetically stirring uniformly to obtain the sodium alginate mixed bacterial liquid, dropwise adding the sodium alginate mixed bacterial liquid into a calcium chloride solution with the mass concentration of 5% by using a 1mL needle tube, wherein the volume ratio of the sodium alginate mixed bacterial liquid to the calcium chloride solution is 1:4, standing, placing in a refrigerator at 4 ℃ for crosslinking reaction for 24 hours, and obtaining the biological carbon MCC220 immobilized naphthalene degrading microbial inoculum (shown in figure 4) after the reaction is finished.
Fully mixing the biochar-immobilized MCC220 immobilized naphthalene degrading microbial inoculum (2g) and polycyclic aromatic hydrocarbon polluted soil (1kg) according to the mass ratio of 2:1000, wherein the mass concentration of naphthalene in the polluted soil is 50mg/L, adding an inorganic salt solution (80mL), uniformly stirring, and then placing in a constant-temperature shaking table at 37 ℃ for shaking culture for 5 days at the shaking speed of 150 r/min. Through determination, the naphthalene degradation removal rate in the polluted soil can reach 80.9%.
Claims (14)
1. A preparation method of a biochar immobilized naphthalene degrading microbial inoculum is characterized by comprising the following steps:
step 1): weighing a biomass raw material, mixing the biomass raw material with water in a polytetrafluoroethylene lining tank, putting the mixture into a stainless steel reaction kettle, heating for reaction, cooling at room temperature after the reaction is finished, carrying out solid-liquid separation, cleaning a solid product by using distilled water and absolute ethyl alcohol, and carrying out freeze drying and grinding to obtain biochar;
step 2): mixing the naphthalene degrading strain subjected to freezing preservation and the biochar obtained in the step 1) in an LB liquid culture medium, and placing the mixture in a constant-temperature shaking table for shaking culture to obtain uniform mixed bacterial liquid;
step 3): mixing the mixed bacterial liquid obtained in the step 2) with a sodium alginate solution, obtaining the sodium alginate mixed bacterial liquid after magnetic stirring is uniform, dropwise adding the sodium alginate mixed bacterial liquid into a calcium chloride solution, standing, placing in a refrigerator at 4 ℃ for crosslinking reaction, and obtaining the biochar immobilized naphthalene degrading microbial inoculum after the reaction is finished.
2. The method for preparing the biochar-immobilized naphthalene-degrading bacterial agent according to claim 1, wherein the biomass raw material in the step 1) is lignin, cellulose or hemicellulose.
3. The preparation method of the biochar-immobilized naphthalene degrading microbial inoculum according to claim 1, wherein the mass ratio of the biomass raw material to water in the step 1) is 1 (10-30).
4. The method for preparing the biochar-immobilized naphthalene degrading microbial inoculum according to claim 1, wherein the heating temperature in the step 1) is 220-280 ℃, and the heating reaction time is 2-4 h; the freeze drying time is 12-24 h.
5. The method for preparing the charcoal-immobilized naphthalene degrading bacterial agent of claim 1, wherein the naphthalene degrading bacterial strain in step 2) is Paenibacillus napus, GenBank accession number is SRR10341461, and naphthalene can be used as a unique carbon source for growth and propagation.
6. The preparation method of the biochar-immobilized naphthalene degrading microbial inoculum according to claim 1, wherein the mass ratio of the naphthalene degrading strain, the biochar and the LB liquid culture medium in the step 2) is 1 (20-50): 250.
7. The method for preparing the biochar-immobilized naphthalene degrading microbial inoculum according to claim 1, wherein the temperature of the constant-temperature shaking table in the step 2) is controlled to be 37 ℃, the oscillation rate is 100-150 r/min, and the oscillation time is 12-24 h.
8. The method for preparing the biochar-immobilized naphthalene degrading bacterial agent according to claim 1, wherein the volume ratio of the mixed bacterial liquid to the sodium alginate solution in the step 3) is 1:1, the mass concentration of the sodium alginate solution is 2% -4%, the mass concentration of the calcium chloride solution is 2% -5%, and the volume ratio of the sodium alginate mixed bacterial liquid to the calcium chloride is 1 (2-4).
9. The preparation method of the biochar-immobilized naphthalene degrading microbial inoculum according to claim 1, wherein the crosslinking reaction time in the step 3) is 12-24 h.
10. A biochar-immobilized naphthalene-degrading bacterial agent prepared by the method of any one of claims 1 to 9.
11. The application of the biochar-immobilized naphthalene degrading microbial inoculum of claim 10 in repairing polycyclic aromatic hydrocarbon-polluted soil.
12. The application of the biochar-immobilized naphthalene-degrading bacterial agent in remediation of soil polluted by polycyclic aromatic hydrocarbons as claimed in claim 11, wherein the application comprises: fully mixing the biochar immobilized naphthalene degrading microbial inoculum with the polycyclic aromatic hydrocarbon polluted soil, simultaneously adding an inorganic salt solution, uniformly stirring, and sampling every 12 hours to determine the concentration of naphthalene residue in the polluted soil.
13. The application of the biochar-immobilized naphthalene degrading microbial inoculum in repairing polycyclic aromatic hydrocarbon-polluted soil according to claim 12, wherein the mass ratio of the biochar-immobilized naphthalene degrading microbial inoculum to the polycyclic aromatic hydrocarbon-polluted soil is 2-3: 1000, and the mass ratio of an inorganic salt solution to the polycyclic aromatic hydrocarbon-polluted soil is 8-15: 100.
14. The application of the biochar-immobilized naphthalene degrading microbial inoculum in repairing polycyclic aromatic hydrocarbon polluted soil according to claim 12, wherein the content of the inorganic salt solution is 1g/L Na2HPO4、0.75g/L KH2PO4、0.5g/L NH4Cl、0.1g/L MgSO4·7H2O、0.01g/L CaCl2、0.5g/L NaNO3、0.03g/L FeSO4·7H2O and 0.005L/L trace element solution with pH value of 7.0-7.2, wherein the content of the trace element solution is 5g/L CuCl2·2H2O、1g/LZnCl2、0.5g/L CoCl2·6H2O and 0.06g/L H3BO3。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911388327.XA CN111088246A (en) | 2019-12-30 | 2019-12-30 | Biochar immobilized naphthalene degrading microbial inoculum and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911388327.XA CN111088246A (en) | 2019-12-30 | 2019-12-30 | Biochar immobilized naphthalene degrading microbial inoculum and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111088246A true CN111088246A (en) | 2020-05-01 |
Family
ID=70397548
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911388327.XA Pending CN111088246A (en) | 2019-12-30 | 2019-12-30 | Biochar immobilized naphthalene degrading microbial inoculum and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111088246A (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109212187A (en) * | 2018-09-06 | 2019-01-15 | 东华大学 | It is a kind of detect polycyclic aromatic hydrocarbon class enzyme linked immunological kit and its application |
| CN111662728A (en) * | 2020-07-29 | 2020-09-15 | 杨凌职业技术学院 | Walnut branch biochar-based fertilizer soil remediation agent and preparation method and application thereof |
| CN111909925A (en) * | 2020-08-13 | 2020-11-10 | 青岛农业大学 | Method for removing soil 2,4, 6-trichlorophenol by porous biomass charcoal immobilized degrading strain |
| CN112795557A (en) * | 2020-12-30 | 2021-05-14 | 天津开发区坤禾生物技术有限公司 | Nutrition-rich immobilized freeze-dried microbial agent and preparation method and application thereof |
| CN114029340A (en) * | 2021-11-09 | 2022-02-11 | 中国科学院南京土壤研究所 | Application of biological PRB of biological carbon coupled microorganism in restoration of polycyclic aromatic hydrocarbon polluted site |
| CN114058507A (en) * | 2021-10-21 | 2022-02-18 | 东莞理工学院 | Carbon-coupled compound microbial inoculum and preparation method and application thereof |
| CN115055515A (en) * | 2022-08-02 | 2022-09-16 | 贵州省生物研究所 | Microbial remediation method for micro-plastics in garbage-polluted soil |
| CN115318812A (en) * | 2022-08-25 | 2022-11-11 | 广东工业大学 | Method for repairing benzo (a) pyrene polluted soil |
| CN116371905A (en) * | 2023-03-20 | 2023-07-04 | 南京农业大学 | Method for removing PAEs in environment by utilizing functional indigenous flora solid microbial inoculum |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101177677A (en) * | 2006-11-08 | 2008-05-14 | 中国科学院沈阳应用生态研究所 | Method for preparing microorganism immobilization embedded particles |
| CN109355278A (en) * | 2018-10-31 | 2019-02-19 | 浙江海洋大学 | A kind of preparation method of the immobilized algal-bacteria beads material for the phosphorus AHs that degrades |
| CN110153170A (en) * | 2019-05-23 | 2019-08-23 | 四川大学 | Composite material, preparation method and application for heavy metal-organic matter reparation |
-
2019
- 2019-12-30 CN CN201911388327.XA patent/CN111088246A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101177677A (en) * | 2006-11-08 | 2008-05-14 | 中国科学院沈阳应用生态研究所 | Method for preparing microorganism immobilization embedded particles |
| CN109355278A (en) * | 2018-10-31 | 2019-02-19 | 浙江海洋大学 | A kind of preparation method of the immobilized algal-bacteria beads material for the phosphorus AHs that degrades |
| CN110153170A (en) * | 2019-05-23 | 2019-08-23 | 四川大学 | Composite material, preparation method and application for heavy metal-organic matter reparation |
Non-Patent Citations (7)
| Title |
|---|
| BAOLIANG CHEN等: "Enhanced bioremediation of PAH-contaminated soil by immobilized bacteria with plant residue and biochar as carriers", 《J SOILS SEDIMENTS》 * |
| 东华大学: "SRR10341461", 《GENBANK》 * |
| 张海荣: "耐盐碱菌的筛选、固定化及在石油烃污染修复中的应用", 《万方数据学位导航》 * |
| 李岩等: "2种秸秆固定化M1对3环PAHs污染土壤修复效果研究", 《河北农业大学学报》 * |
| 李骅等: "海藻酸钠－生物炭联合固定化菌株降解２－羟基－１,４－萘醌", 《南京农业大学学报》 * |
| 王艳等: "生物降解萘的研究进展", 《中国生物工程杂志》 * |
| 黄占斌: "《环境材料学》", 30 November 2017 * |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109212187A (en) * | 2018-09-06 | 2019-01-15 | 东华大学 | It is a kind of detect polycyclic aromatic hydrocarbon class enzyme linked immunological kit and its application |
| CN109212187B (en) * | 2018-09-06 | 2022-01-11 | 东华大学 | ELISA-like kit for detecting polycyclic aromatic hydrocarbons and application thereof |
| CN111662728A (en) * | 2020-07-29 | 2020-09-15 | 杨凌职业技术学院 | Walnut branch biochar-based fertilizer soil remediation agent and preparation method and application thereof |
| CN111662728B (en) * | 2020-07-29 | 2021-12-21 | 杨凌职业技术学院 | Walnut branch biochar-based fertilizer soil remediation agent and preparation method and application thereof |
| CN111909925A (en) * | 2020-08-13 | 2020-11-10 | 青岛农业大学 | Method for removing soil 2,4, 6-trichlorophenol by porous biomass charcoal immobilized degrading strain |
| CN112795557A (en) * | 2020-12-30 | 2021-05-14 | 天津开发区坤禾生物技术有限公司 | Nutrition-rich immobilized freeze-dried microbial agent and preparation method and application thereof |
| CN114058507A (en) * | 2021-10-21 | 2022-02-18 | 东莞理工学院 | Carbon-coupled compound microbial inoculum and preparation method and application thereof |
| CN114029340A (en) * | 2021-11-09 | 2022-02-11 | 中国科学院南京土壤研究所 | Application of biological PRB of biological carbon coupled microorganism in restoration of polycyclic aromatic hydrocarbon polluted site |
| CN115055515A (en) * | 2022-08-02 | 2022-09-16 | 贵州省生物研究所 | Microbial remediation method for micro-plastics in garbage-polluted soil |
| CN115318812A (en) * | 2022-08-25 | 2022-11-11 | 广东工业大学 | Method for repairing benzo (a) pyrene polluted soil |
| CN116371905A (en) * | 2023-03-20 | 2023-07-04 | 南京农业大学 | Method for removing PAEs in environment by utilizing functional indigenous flora solid microbial inoculum |
| CN116371905B (en) * | 2023-03-20 | 2023-10-13 | 南京农业大学 | Method for removing PAEs in environment by utilizing functional indigenous flora solid microbial inoculum |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111088246A (en) | Biochar immobilized naphthalene degrading microbial inoculum and preparation method and application thereof | |
| CN109628353B (en) | Biochar-based immobilized microbial agent as well as preparation method and application thereof | |
| CN109536173B (en) | Composite material for simultaneously repairing heavy metal and glyphosate and preparation method thereof | |
| CN108130299B (en) | Microbial strain for degrading petroleum hydrocarbon and combined repairing agent | |
| CN104475444A (en) | Preparation, application and usage method for biocarbon-immobilized composite pollution-degrading bacterium particle | |
| CN109794501B (en) | Method for preparing compound soil by using petroleum hydrocarbon polluted soil | |
| CN103484447A (en) | Preparation method and application of petroleum degrading enzyme preparation | |
| CN108611292A (en) | The preparation method and application of cold-resistant ammonia oxidizing bacteria immobilization biological charcoal ball | |
| CN111733098B (en) | Application of bacillus in low-temperature degradation of petroleum hydrocarbon | |
| CN107151663B (en) | Immobilized microbial agent prepared from kelp residue and used for petroleum pollution remediation | |
| CN108048365B (en) | 2, 4-dinitrotosylate degrading strain and application thereof | |
| CN111995066A (en) | Method for treating petrochemical industry wastewater by adopting biological enhanced microbial inoculum adsorption-embedding mode | |
| Mikolasch et al. | From oil spills to barley growth–oil‐degrading soil bacteria and their promoting effects | |
| CN105647838A (en) | Acinetobacter pittii and application thereof | |
| Dong et al. | Optimization of growth conditions and biological cementation effect of Sporosarcina pasteurii | |
| Ren et al. | Enhanced biodegradation of oil-contaminated soil oil in shale gas exploitation by biochar immobilization | |
| CN104450669A (en) | Method for degrading trichloroethylene by virtue of biological carbon immobilized microorganism co-metabolism | |
| CN104845899A (en) | Application of Rhodococcus sp. 2G in degradation of phthalate | |
| CN106497859A (en) | A kind of method that utilization conductive carrier tames propanoic acid methanogen film | |
| Jain | Suitability of various ureolytic microbes for potential soil strengthening via biocementation | |
| CN115505587A (en) | Preparation method and application of modified kaolin immobilized pyrene degradation microbial inoculum | |
| Wen et al. | Immobilization of Bacillus Thuringiensis and applicability in removal of sulfamethazine from soil | |
| CN110818180A (en) | Method for digesting black and odorous river channel by using microbial preparation | |
| Gandara | Publication Frequency | |
| CN114134079B (en) | Tetracycline antibiotic degrading bacteria, method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200501 |