CN111072756B - Tetrodotoxin ACE inhibitory peptide and preparation method thereof - Google Patents
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Abstract
The invention discloses a puffer fish ACE inhibitory peptide and a preparation method thereof, belongs to the technical field of ACE inhibitory peptide, and the preparation method of the puffer fish ACE inhibitory peptide comprises the following steps: s1: preparing the puffer fish enzymatic hydrolysate, S2: and (3) performing ultrafiltration separation on the puffer fish enzymatic hydrolysate, wherein the step S3: separating and purifying the polypeptide, S4, identifying the sequence of the polypeptide, S5, synthesizing the polypeptide and measuring the activity. A tetrodotoxin ACE inhibitory peptide is nonapeptide, and the amino acid sequence is as follows: GDRGFPGER (Gly-Asp-Arg-Gly-Phe-Pro-Gly-Glu-Arg). The invention relates to a puffer fish ACE inhibitory peptide and a preparation method thereof, wherein the puffer fish skin is used as a raw material to obtain a nonapeptide amino acid sequence, and the prepared polypeptide has ACE inhibitory activity.
Description
Technical Field
The invention belongs to the technical field of ACE inhibitory peptides, and particularly relates to an ACE inhibitory peptide of puffer fish and a preparation method thereof.
Background
With the progress and development of society, the living standard of people is gradually improved, the proportion of fat and heat in diet is gradually increased, and the exercise amount is reduced, so that the incidence of cardiovascular diseases in China is rapidly increased, wherein hypertension is the main factor.
The puffer fish is a fish in the warm temperate zone and the tropical near-sea bottom layer, inhabits the middle and lower layers of the sea, has few species entering the fresh water river, and makes the whole body float on the water surface in a spherical shape when encountering external danger, and small thorns on the skin are erected to defend the puffer fish.
Angiotensin Converting Enzyme (ACE) inhibitory peptide is a small molecular polypeptide formed by proteolysis, has a remarkable blood pressure lowering effect, and more effectively is found to have no toxic or side effect and no influence on normal blood pressure compared with other common blood pressure lowering medicines. ACE plays an important role in blood pressure regulation, and through the removal of two amino acids (His-Leu) at the carbon end, originally inactive angiotensin I can be converted into active angiotensin II, so that vasoconstriction is caused, and the blood pressure is increased; the ACE also can lead the relaxing skin with the vasodilatation function to be inactivated and also can cause the situation of blood pressure rise, and the ACE inhibitory peptide can block two biochemical reaction processes caused by the ACE to play the role of reducing the blood pressure.
The prior art for preparing ACE inhibitory peptide from foods such as hazelnuts, yak milk, shellfish meat, medlar and the like does not refer to the technology for preparing ACE inhibitory peptide from puffer fish, particularly puffer fish skin.
Disclosure of Invention
The invention aims to provide a puffer fish ACE inhibitory peptide and a preparation method thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention provides an ACE inhibitory peptide of puffer fish, which is nonapeptide and has the amino acid sequence as follows: GDRGFPGER (Gly-Asp-Arg-Gly-Phe-Pro-Gly-Glu-Arg).
Preferably, nonapeptide GDRGFPGER (Gly-Asp-Arg-Gly-Phe-Pro-Gly-Glu-Arg) has a molecular weight of 989.5 Da.
Preferably, the IC of nonapeptide GDRGFPGER (Gly-Asp-Arg-Gly-Phe-Pro-Gly-Glu-Arg)50The value was 9.0 to 9.4 umol/mL.
The invention also provides a preparation method of the tetraodon ACE inhibitory peptide, which comprises the following steps: s1: preparing the puffer fish enzymatic hydrolysate, pulping puffer fish skins, adding water, stirring to form a protein solution, adjusting the pH value to be alkaline, adding alkaline protease for enzymolysis, keeping the pH value unchanged, performing enzyme deactivation treatment after the enzymolysis is finished, cooling to room temperature, adjusting the pH value to be neutral, centrifuging to obtain a supernatant, freeze-drying the supernatant to obtain crude puffer fish ACE inhibitory peptide, adding the freeze-dried crude puffer fish ACE inhibitory peptide into water to prepare the puffer fish enzymatic hydrolysate, S2: performing ultrafiltration separation on the puffer fish enzymatic hydrolysate, performing clarification separation on the puffer fish enzymatic hydrolysate, performing ultrafiltration treatment on the clarified puffer fish enzymatic hydrolysate, respectively collecting separation components with different sizes and molecular weights, freeze-drying, and determining the ACE inhibition rate of each separation component, S3: separating and purifying polypeptide, primarily separating and purifying separated components with the molecular weight of less than 1kDa by using semi-preparative liquid chromatography, collecting 8 different absorption peak components, collecting eluent according to the time range of the absorption peaks, respectively naming the eluent as A1-A8 components, then carrying out ACE activity determination, selecting A5 components and carrying out gel filtration separation, then carrying out high performance liquid chromatography on the A5 components after gel filtration separation for further separation and purification, separating to obtain high-purity polypeptide components, S4, carrying out sequence identification on the polypeptide, carrying out mass spectrum determination on the high-purity polypeptide components obtained by separation in the step S3, analyzing the result of the mass spectrum determination to obtain nonapeptide GDRGFPGER (Gly-Asp-Arg-Gly-Phe-Pro-Gly-Glu-Arg), S5, carrying out polypeptide synthesis and activity determination, and carrying out nonapeptide GDRGFPGER (Gly-Asp-Arg-Gly-Phe-Pro-Gly-Glu-Arg) And (3) carrying out solid phase synthesis according to the determined polypeptide sequence, and verifying the ACE inhibitory activity of the synthesized polypeptide.
Preferably, the specific steps of step S1 are: pulping 80-120g of puffer fish skin, adding 250-350mL of distilled water, stirring to form a protein solution, adjusting the pH value to 7.5-9.0 by NaOH, adding alkaline protease with the enzyme amount of 450-350U/g, carrying out enzymolysis for 6-10h at 50-60 ℃, keeping the pH value unchanged, heating at 80-100 ℃ for 12-18min after the enzymolysis is finished, carrying out enzyme deactivation treatment, cooling to room temperature, adjusting the pH value to be neutral by HCl, centrifuging at the speed of 4000-6000r/min for 8-12min, taking supernatant, freeze-drying the supernatant to obtain crude puffer ACE inhibitory peptide, and adding the freeze-dried crude puffer ACE inhibitory peptide into distilled water to prepare 0.8-1.2mg/mL of puffer fish enzymatic hydrolysate.
Preferably, the specific steps of step S2 are: and (4) clarifying and separating the puffer fish enzymatic hydrolysate prepared in the step (S1) by adopting a ceramic membrane with the aperture of 50-100 mu m, then carrying out ultrafiltration treatment on the clarified puffer fish enzymatic hydrolysate by adopting polyether sulfone ultrafiltration membranes with the aperture of 50kDa, 30kDa, 10kDa, 5kDa and 1kDa, respectively collecting separation components with different molecular weights, freeze-drying and determining the ACE inhibition rate of each separation component.
Preferably, the semi-preparative liquid chromatography of step S3 is performed by using a 2.5cm × 20cm Sinochrom ODS-BP chromatographic column, the sample loading amount is 3-5mL, the buffer solution is subjected to isocratic elution by using a 10% methanol solution, the elution flow rate is 5mL/min, the detection is performed by using an ultraviolet detector, the ultraviolet detection wavelength is 220nm, 8 components are collected, and the freeze-drying is performed.
Preferably, the gel filtration separation of step S3 is performed by using a glass chromatography column with a diameter of 20X 1000mm, a SephadexG-15 packing, a height of 800-1000mm packing, a detection wavelength of 220nm, pure water as a mobile phase, a flow rate of 4.5-5.5mL/min as a mobile phase, and a temperature of room temperature.
Preferably, the high performance liquid chromatography of step S3 employs sunfire C of 4.6X 250mm18A chromatographic column, wherein the ultraviolet detection wavelength is 220nm, the mobile phase A is pure water containing TFA with the mass fraction of 0.4-0.6%, the mobile phase B is acetonitrile, the volume ratio of the mobile phase A to the mobile phase B is 75:25 in equilibrium, the flow rate of the mobile phase is 0.8-1.2mL/min, and the temperature is 25 ℃.
Preferably, step S4 is performed by LC-MS/MS mass spectrometry under the following conditions: the chromatographic column is PepMap RPLC C1875 μm i.d. × 150mm, 3 μm, 100) cation mode, scan range: m/z 300-1500 Da, emitter spray voltage 2-kV, and the result of mass spectrometry is analyzed by PEAKS STUDIO software to obtain the polypeptide amino acid sequence.
The invention has the beneficial effects that:
1. the nonapeptide amino acid sequence is obtained by using puffer fish skin as raw material, and the prepared polypeptide has ACE inhibiting activity.
2. A hydrogen bond network is formed between nonapeptide GDRGFPGER (Gly-Asp-Arg-Gly-Phe-Pro-Gly-Glu-Arg) and ACE, and the stability is high.
3. Nonapeptide GDRGFPGER (Gly-Asp-Arg-Gly-Phe-Pro-Gly-Glu-Arg) Asp2 backbone amide oxygen atom has electronegativity and can be combined with Zn of binding site2+Forming stronger polar effect, thereby destroying the original function of Zn ions and improving the inhibition effect.
4. Nonapeptide GDRGFPGER (Gly-Asp-Arg-Gly-Phe-Pro-Gly-Glu-Arg) has more hydrophobic residues which can form stronger hydrophobic interactions and van der Waals potentials with binding pocket residues.
Drawings
FIG. 1 is a graph showing the determination of ACE inhibitory ratio by three protease enzymatic hydrolysates.
FIG. 2 is the IC of ACE inhibition of fractions of different molecular weight fractions collected in step S2 of the present invention50Value measurement graph.
FIG. 3 is a graph showing the determination of ACE inhibitory activity of different molecular weight fractions collected in step S2 according to the present invention.
FIG. 4 is a semi-preparative liquid chromatography separation profile of the present invention.
FIG. 5 is a graph showing the ACE inhibitory activity of 8 absorption components collected by semi-preparative liquid chromatography in step S3 according to the present invention.
FIG. 6 is a diagram of the polypeptide fraction purified by SephadexG-15 in step 3 of the present invention.
FIG. 7 is a liquid chromatography chromatogram of a high purity polypeptide of the present invention.
FIG. 8 is a high purity polypeptide sequencing map of the present invention.
FIG. 9 is a graph of an assay for ACE inhibitory activity of the synthetic polypeptides of the present invention.
FIG. 10 is a pattern of binding of the synthetic polypeptides of the invention to ACE.
FIG. 11 shows the inhibition pattern and mechanism of action of the synthetic polypeptides of the invention with ACE.
Detailed Description
The invention will now be further described with reference to the accompanying drawings and detailed description.
The present embodiment provides a fugu ACE inhibitory peptide, wherein the inhibitory peptide is nonapeptide, and the amino acid sequence: GDRGFPGER (Gly-Asp-Arg-Gly-Phe-Pro-Gly-Glu-Arg).
Nonapeptide GDRGFPGER (Gly-Asp-Arg-Gly-Phe-Pro-Gly-Glu-Arg) has a molecular weight of 989.5 Da.
The embodiment also provides a preparation method of the takifugu ACE inhibitory peptide, which comprises the following steps:
s1: the method comprises the steps of pulping 100g of puffer fish skin, adding 300mL of distilled water, stirring to form a protein solution, adjusting the pH value to 8.0 by NaOH, adding 500U/g of alkaline protease, performing enzymolysis for 10h at 55 ℃, keeping the pH value unchanged, heating for 15min at 90 ℃ after enzymolysis is finished, performing enzyme deactivation treatment, cooling to room temperature, adjusting the pH value to be neutral by HCl, centrifuging for 10min at 5000r/min, taking supernatant, freeze-drying the supernatant to obtain puffer fish ACE inhibitory peptide, storing at 4 ℃, adding the freeze-dried puffer fish ACE inhibitory peptide into distilled water, and preparing the puffer fish ACE inhibitory peptide into 1mg/mL of puffer fish enzymatic hydrolysate.
As shown in fig. 1, the protease was obtained as 3 commercial proteases: pepsin (the optimum pH and the temperature are pH5.7 and 60 ℃), neutral protease (the optimum pH and the temperature are pH7.0 and 50 ℃), alkaline protease (the optimum pH and the temperature are pH8.0 and 55 ℃) are used as enzyme preparation screening sources, the influence of the neutral protease on the ACE inhibition rate of the hydrolysis of the skin of the Takifugu flavidus under the optimum conditions is researched, and the protease suitable for producing the ACE inhibitory peptide by the proteolysis of the skin of the Takifugu flavidus is screened. The results show that the use of alkaline protease has the best enzymolysis effect. The enzymolysis conditions are as follows: alkaline protease enzyme concentration: 500U/g, the pH value is adjusted to 8.0 by sodium hydroxide (NaOH), the enzymolysis temperature is 55 ℃, and the enzymolysis time is 10 h.
S2: and (4) performing ultrafiltration separation on the puffer fish enzymatic hydrolysate prepared in the step (S1), namely performing clarification separation on the puffer fish enzymatic hydrolysate by adopting a ceramic membrane with the pore diameter of 50 mu m, performing ultrafiltration treatment on the clarified puffer fish enzymatic hydrolysate by adopting polyether sulfone ultrafiltration membranes with the pore diameters of 50kDa, 30kDa, 10kDa, 5kDa and 1kDa, respectively collecting separation components with different molecular weights, freeze-drying the separation components, and determining IC (integrated Circuit) of ACE (angiotensin converting enzyme) inhibition rate of each separation component50The value is obtained. The results showed that the fraction with a molecular weight of less than 1kDa had the highest ACE inhibitory rate as shown in FIG. 2, and the fraction with a molecular weight of less than 1kDa had the highest ACE inhibitory activity as shown in FIG. 3, and therefore, the fraction with a molecular weight of less than 1kDa was selected for the next stepAnd (5) purifying.
S3: separating and purifying polypeptide, performing primary separation and purification on separated components with molecular weight less than 1kDa by semi-preparative liquid chromatography, collecting 8 different absorption peak components, and collecting eluates according to the time range of the absorption peak, which are respectively named as A1-A8 components, as shown in figure 4. And then, performing ACE activity determination, as shown in figure 5, wherein A4, A5, A7 and A8 have high ACE inhibitory activity, the method selects the A5 component and performs gel filtration separation on the component, and then performs high performance liquid chromatography on the A5 component after the gel filtration separation for further separation and purification, and the high-purity polypeptide component is obtained through separation.
Specifically, the semi-preparative liquid chromatography of step S3 adopts a 2.5cm × 20cm Sinochrom ODS-BP chromatographic column, the sample loading amount is 5mL, the buffer solution adopts 10% methanol solution for isocratic elution, the elution flow rate is 5mL/min, and the detection is carried out in an ultraviolet detector, the ultraviolet detection wavelength is 220nm, 8 components are collected, and the freeze drying is carried out.
Specifically, the gel filtration and separation in step S3 is performed by using a glass chromatographic column with a diameter of 20 × 1000mm, the filler is sephadex g-15, the height of the filler is 900mm, the detection wavelength is 220nm, the mobile phase is pure water, the flow rate of the mobile phase is 5mL/min, the temperature is room temperature, and as shown in fig. 6, the gel filtration and separation is a graph of the purified polypeptide fraction passing through sephadex g-15.
Specifically, the high performance liquid chromatography of step S3 used SunfireC of 4.6X 250mm18A chromatographic column, the ultraviolet detection wavelength is 220nm, the mobile phase A is pure water containing TFA with the mass fraction of 0.5%, the mobile phase B is acetonitrile, the volume ratio of the mobile phase A to the mobile phase B is 75:25 in equilibrium, the flow rate of the mobile phase is 1mL/min, the temperature is 25 ℃, and as shown in FIG. 7, the chromatographic spectrum of the high-purity polypeptide liquid phase is shown.
S4, identifying the sequence of the polypeptide, performing mass spectrometry on the high-purity polypeptide component obtained by separation in the step S3, analyzing the mass spectrometry result to obtain nonapeptide GDRGFPGER (Gly-Asp-Arg-Gly-Phe-Pro-Gly-Glu-Arg), and specifically performing mass spectrometry by adopting LC-MS/MS under the following conditions: the chromatographic column is PepMap RPLC C1875 μm i.d. × 150mm, 3 μm, 100) cation mode, scan range: m/z 300-1500 Da, emissionThe electrode spray voltage is 2-kV, the result of mass spectrometry is analyzed by PEAKS STUDIO software, and a polypeptide amino acid sequence GDRGFPGER is obtained, and the sequence determination map is shown in figure 8.
S5 polypeptide synthesis and activity determination, the method comprises the steps of carrying out solid phase synthesis on nonapeptide GDRGFPGER (Gly-Asp-Arg-Gly-Phe-Pro-Gly-Glu-Arg) according to a determined polypeptide sequence, and verifying ACE inhibitory activity of the synthesized polypeptide. The results showed that the purified polypeptide had good ACE inhibitory activity, and the IC of the nonapeptide GDRGFPGER (Gly-Asp-Arg-Gly-Phe-Pro-Gly-Glu-Arg) obtained50The value was 9.2 umol/mL. The ACE inhibitory activity of the synthetic polypeptides was determined as shown in figure 9.
The Discovery Studio software is used for carrying out simulation analysis on the docking mechanism of the polypeptide and the ACE, and the results are as follows:
as shown in fig. 10-11, a hydrogen bonding network was formed between nonapeptide GDRGFPGER and ACE. Both share 12 hydrogen bonding interactions. These hydrogen bonding effects are critical for stable binding of the polypeptide to ACE. Binding pocket residues that hydrogen bond with polypeptides include: ala356, His353, His513, Gln281, Thr282, etc. In addition, the peptide Asp2 has electronegativity on the amide oxygen atom of the backbone, and can be combined with Zn of a binding site2+Forming stronger polar action, thereby destroying the original functions of Zn ions. Therefore, it also plays a role in exerting the polypeptide inhibitory action. In addition, the binding pocket has multiple hydrophobic residues, which can form strong hydrophobic interactions and van der waals potential with the polypeptide. These more hydrophobic binding pocket residues include: phe457, Val379, Phe527, Phe512, and the like.
Those not described in detail in this specification are within the skill of the art; the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those skilled in the art; the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
SEQUENCE LISTING
<110> research institute for aquatic products of Fujian province (center for preventing and treating diseases of Fujian aquatic products)
<120> an ACE inhibitory peptide of puffer fish and a preparation method thereof
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 9
<212> PRT
<213> Takifugu flavidus
<400> 1
Gly Asp Arg Gly Phe Pro Gly Glu Arg
1 5
Claims (10)
1. An ACE inhibitory peptide of a puffer fish,
the inhibitory peptide is nonapeptide, and the amino acid sequence:
GDRGFPGER(Gly-Asp-Arg-Gly-Phe-Pro-Gly-Glu-Arg)。
2. the tetrodotoxin ACE inhibitory peptide of claim 1,
the nonapeptide GDRGFPGER (Gly-Asp-Arg-Gly-Phe-Pro-Gly-Glu-Arg) has a molecular weight of 989.5 Da.
3. The tetrodotoxin ACE inhibitory peptide of claim 1,
IC of the nonapeptide GDRGFPGER (Gly-Asp-Arg-Gly-Phe-Pro-Gly-Glu-Arg)50The value was 9.0 to 9.4 umol/mL.
4. A method for preparing an ACE inhibitory peptide of a puffer fish is characterized by comprising the following steps:
s1: preparation of fugu enzymatic hydrolysate
Pulping the skin of a puffer fish, adding water, stirring to form a protein solution, adjusting the pH value to be alkaline, adding alkaline protease for enzymolysis, keeping the pH value unchanged, performing enzyme deactivation treatment after the enzymolysis is finished, cooling to room temperature, adjusting the pH value to be neutral, centrifuging to obtain a supernatant, freeze-drying the supernatant to obtain crude puffer fish ACE inhibitory peptide, and adding the freeze-dried crude puffer fish ACE inhibitory peptide into water to prepare puffer fish enzymolysis liquid;
s2: ultra-filtration separation of fugu enzymatic hydrolysate
Clarifying and separating the puffer fish enzymatic hydrolysate, then performing ultrafiltration treatment on the clarified puffer fish enzymatic hydrolysate, respectively collecting separation components with different molecular weights, freeze-drying, and determining the ACE inhibition rate of each separation component;
s3: separation and purification of polypeptide
Primarily separating and purifying the separated components with the molecular weight of less than 1kDa by using a semi-preparative liquid chromatography, collecting 8 different absorption peak components, collecting eluent according to the time range of the absorption peaks, respectively naming the eluent as A1-A8 components, then carrying out ACE activity determination, selecting and carrying out gel filtration and separation on the A5 component, then carrying out high performance liquid chromatography on the A5 component after the gel filtration and separation for further separation and purification, and separating to obtain a high-purity polypeptide component;
s4 sequence identification of the polypeptide
Subjecting the high-purity polypeptide fraction separated in step S3 to mass spectrometry, and analyzing the mass spectrometry result to obtain nonapeptide GDRGFPGER (Gly-Asp-Arg-Gly-Phe-Pro-Gly-Glu-Arg);
s5 polypeptide Synthesis and Activity determination
The nonapeptide GDRGFPGER (Gly-Asp-Arg-Gly-Phe-Pro-Gly-Glu-Arg) was subjected to solid phase synthesis according to the determined polypeptide sequence to verify the ACE inhibitory activity of the synthesized polypeptide.
5. The method for preparing tetrodotoxin ACE inhibitory peptide as defined in claim 4,
the specific steps of step S1 are:
pulping 80-120g of puffer fish skin, adding 250-350mL of distilled water, stirring to form a protein solution, adjusting the pH value to 7.5-9.0 by NaOH, adding alkaline protease with the enzyme amount of 450-350U/g, carrying out enzymolysis for 6-10h at 50-60 ℃, keeping the pH value unchanged, heating at 80-100 ℃ for 12-18min after the enzymolysis is finished, carrying out enzyme deactivation treatment, cooling to room temperature, adjusting the pH value to be neutral by HCl, centrifuging at the speed of 4000-6000r/min for 8-12min, taking supernatant, freeze-drying the supernatant to obtain crude puffer ACE inhibitory peptide, and adding the freeze-dried crude puffer ACE inhibitory peptide into distilled water to prepare 0.8-1.2mg/mL of puffer fish enzymatic hydrolysate.
6. The method for preparing tetrodotoxin ACE inhibitory peptide as defined in claim 4,
the specific steps of step S2 are:
and (4) clarifying and separating the puffer fish enzymatic hydrolysate prepared in the step (S1) by adopting a ceramic membrane with the aperture of 50-100 mu m, then carrying out ultrafiltration treatment on the clarified puffer fish enzymatic hydrolysate by adopting polyethersulfone ultrafiltration membranes with the apertures of 50kDa, 30kDa, 10kDa, 5kDa and 1kDa, respectively collecting separation components with different molecular weights, freeze-drying and determining the ACE inhibition rate of each separation component.
7. The method for preparing tetrodotoxin ACE inhibitory peptide as defined in claim 4,
the semi-preparative liquid chromatography of the step S3 adopts a 2.5cm × 20cm Sinochrom ODS-BP chromatographic column, the sample loading amount is 3-5mL, the buffer solution adopts 10% methanol solution for isocratic elution, the elution flow rate is 5mL/min, the detection is carried out in an ultraviolet detector, the ultraviolet detection wavelength is 220nm, 8 components are collected and freeze-dried.
8. The method for preparing tetrodotoxin ACE inhibitory peptide as defined in claim 4,
the gel filtration and separation in the step S3 adopts a glass chromatographic column with the diameter of 20 multiplied by 1000mm, the filler is SephadexG-15, the height of the filler is 800-1000mm, the detection wavelength is 220nm, the mobile phase is pure water, the flow rate of the mobile phase is 4.5-5.5mL/min, and the temperature is room temperature.
9. The method for preparing tetrodotoxin ACE inhibitory peptide as defined in claim 4,
the high performance liquid chromatography of the step S3 adopts sunfire C of 4.6X 250mm18Chromatographic column, ultraviolet detection wavelengthAt 220nm, mobile phase A is pure water containing TFA with mass fraction of 0.4-0.6%, mobile phase B is methanol, and when in equilibrium, 10% -50% B is eluted linearly at the flow rate of 0.5-1mL/min and the temperature of 25 ℃.
10. The method for preparing tetrodotoxin ACE inhibitory peptide as defined in claim 4,
the step S4 adopts LC-MS/MS to carry out mass spectrometry, and the measurement conditions are as follows: the chromatographic column is PepMap RPLC C1875 μm i.d. × 150mm, 3 μm, 100) cation mode, scan range: m/z 300-1500 Da, emitter spray voltage 2-kV, and the result of mass spectrometry is analyzed by PEAKS STUDIO software to obtain the polypeptide amino acid sequence.
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CN113603745B (en) * | 2021-08-09 | 2023-07-07 | 福建省水产研究所(福建水产病害防治中心) | Active fugu flavidus fish skin polypeptide and preparation method and application thereof |
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