CN111061053A - Micro optical tweezers device and method based on self-focusing lens array - Google Patents
Micro optical tweezers device and method based on self-focusing lens array Download PDFInfo
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- CN111061053A CN111061053A CN202010056457.XA CN202010056457A CN111061053A CN 111061053 A CN111061053 A CN 111061053A CN 202010056457 A CN202010056457 A CN 202010056457A CN 111061053 A CN111061053 A CN 111061053A
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- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
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- G—PHYSICS
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Abstract
The invention discloses a micro optical tweezers device and method based on a self-focusing lens array, which sequentially comprises the following steps according to the light propagation direction: the device comprises a laser, an optical fiber, a collimator, a micro-lens array and a self-focusing lens array, wherein a tightly focused light beam is formed and extends into a culture dish to grab a sample; the invention generates a plurality of optical tweezers grabbing trap positions on the working surface of the self-focusing lens, can simultaneously sort and operate a plurality of samples, and expands the application range of the optical tweezers.
Description
Technical Field
The invention belongs to the technical field of optical control, and relates to a micro optical tweezers device and a method based on a self-focusing lens array.
Background
Focused laser beams can "clamp" and manipulate microscopic particles (atoms, molecules, etc.) and microscopic objects (cells, viruses, etc.) like Tweezers, and this technique, called "Optical Tweezers" (Optical Tweezers), has important applications in the field of particle physics and life sciences. The micro optical tweezers can get rid of the space limitation of a light beam focusing element in the traditional optical tweezers, and can be conveniently integrated in high-end microscopic imaging equipment for cell sorting and control.
The light beam focusing element in the traditional optical tweezers is limited by space and cannot be integrated in high-end microscopic imaging equipment for cell sorting and control; the traditional optical tweezers only usually generate one beam of focusing light beam, and only a single sample can be operated at one time; and the traditional optical tweezers are poor in repeatability, flexible and easy to deform, and the capturing force of the optical tweezers cannot be guaranteed.
Therefore, it is an urgent technical problem to be solved by those skilled in the art to provide a micro optical tweezers device and method that can be conveniently integrated into high-end microscopic imaging equipment and can simultaneously manipulate multiple samples while ensuring the trapping force of the optical tweezers.
Disclosure of Invention
The invention realizes the three-dimensional control of a plurality of suspended particles or cells under an optical microscope. The micro optical tweezers device is constructed by utilizing the high-numerical-aperture self-focusing lens array, the space limitation of a light beam focusing element in the traditional optical tweezers can be avoided, the micro optical tweezers can be conveniently integrated in high-end microscopic imaging equipment to perform cell sorting and control, a plurality of samples can be simultaneously controlled, and the micro optical tweezers are compared with the optical tweezers which are poor in repeatability and flexible and easy to deform, so that the trapping force of the optical tweezers is ensured.
The specific scheme for achieving the purpose is as follows:
the utility model provides a miniature optical tweezers device based on self-focusing lens array, is according to the light propagation direction in proper order: the device comprises a laser, an optical fiber, a collimator, a micro-lens array and a self-focusing lens array, wherein a focusing light beam is formed and extends into a culture dish to grab a sample; the sample is placed in a liquid environment of the culture dish, the liquid environment comprising a culture fluid.
Preferably, the microlens array integrates N microlenses, where N is greater than or equal to 3, to generate N focused beams, and the number of focused beams is the same as the number of high numerical aperture self-focusing lenses in the self-focusing lens array.
Preferably, the front working surface of the self-focusing lens array is placed at the focal plane of the microlens array.
Preferably, the self-focusing lens array is formed by symmetrically combining N high-numerical-aperture self-focusing lenses according to the same interval angle, and a tightly-focused light beam with a high numerical aperture is formed and extends into the culture dish to manipulate a plurality of samples simultaneously.
Preferably, the N high numerical aperture self-focusing lenses in the self-focusing lens array are arranged corresponding to the N microlens positions in the microlens array.
Preferably, the sample is a nearly spherical sample to be tested suspended in a culture dish, and comprises single cells, cell groups or particles with the maximum diameter of micron or nanometer.
Preferably, the size specification of the self-focusing lens is
The length is 3.4 mm.
The invention also provides a method for realizing the micro optical tweezers based on the self-focusing lens array, which comprises the following steps:
a laser beam emitted by a laser forms a parallel laser beam through an optical fiber and a collimator, the parallel laser beam is incident on a micro-lens array to generate N beams of focusing light, wherein N is greater than or equal to 3, the focusing light is coupled into a self-focusing lens array immediately to form a tightly-focused beam with a high numerical aperture, and the focusing beam extends into a culture solution of a culture dish to grab a sample.
Preferably, the N focused lights are respectively incident into N high numerical aperture self-focusing lenses of the self-focusing lens array.
Preferably, the focused light beam is incident on the front working surface of the self-focusing lens array at the focal plane of the micro-lens array.
Compared with the prior art, the invention has the following beneficial effects:
firstly, the invention utilizes a group of high numerical aperture self-focusing lens arrays to construct a micro optical tweezers device, overcomes the technical defects that a light beam focusing element in the traditional optical tweezers is a water mirror/oil mirror with high numerical aperture, the size is larger (approximately 5mm 20mm), and the objective lens needs a corresponding clamping and fixing device, the device cannot move and the size cannot be reduced, can not only relieve the space limitation of the light beam focusing element in the traditional optical tweezers, is convenient to integrate into high-end microscopic imaging equipment for cell sorting and control, but also can simultaneously operate a plurality of samples, and compared with the fiber optical tweezers with poor repeatability and softness and easy deformation, the invention ensures the capturing force of the optical tweezers and greatly expands the application range of the optical tweezers;
and secondly, compared with the focusing light beam generated by the low numerical aperture micro lens array, the focusing light beam generated by the high numerical aperture self-focusing lens array is used, so that the capturing force of the optical tweezers is ensured.
And thirdly, the micro optical tweezers device can conveniently extend into the culture solution, a plurality of optical tweezers grabbing trap positions are generated on the working surface of the self-focusing lens, and a plurality of suspended cells are grabbed.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It is obvious that the drawings in the following description are only embodiments of the invention, and that for a person skilled in the art, other drawings can be obtained from the provided drawings without inventive effort.
FIG. 1 is a schematic structural diagram of a micro optical tweezers device based on a self-focusing lens array according to the present invention;
FIG. 2 is a cross-sectional view of a microlens array provided by the present invention;
fig. 3 is a cross-sectional view of a self-focusing lens array provided by the present invention.
In the figure, the position of the upper end of the main shaft,
1 is a laser, 2 is an optical fiber, 3 is a collimator, 4 is a micro-lens array, 5 is a self-focusing lens array, 6 is a sample, and 7 is a culture dish.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The structure diagram of the invention is shown in fig. 1, and the invention relates to a micro optical tweezers device based on a self-focusing lens array, which utilizes a 3D printing technology to construct a core component of a personalized micro optical tweezers device, and uses a high numerical aperture self-focusing lens array to generate a high numerical aperture tightly-focused light beam for cell sorting and control integrated in high-end microscopic imaging equipment. The light propagation direction is as follows in sequence: the device comprises a laser 1, an optical fiber 2, a collimator 3, a micro-lens array 4 and a self-focusing lens array 5, wherein a focusing light beam is formed and extends into a culture dish 7 to grab a sample 6; the sample 6 is placed in a liquid environment of a culture dish 7, the liquid environment comprising a culture liquid.
In order to further optimize the technical scheme, the output optical fiber end of the laser is connected with an optical fiber coupler and then connected to a collimator, and the optical fiber coupler is formed by 3D printing, can be moved easily and is small in size.
In order to further optimize the above technical solution, the microlens array 4 integrates N microlenses, where N is greater than or equal to 3, to generate N focused beams, and the number of the focused beams is the same as the number of the high numerical aperture self-focusing lenses in the self-focusing lens array 4.
To further optimize the above solution, the front working surface of the self-focusing lens array 5 is placed at the focal plane of the micro-lens array 4.
In order to further optimize the technical scheme, the self-focusing lens array 5 is formed by symmetrically combining N high-numerical-aperture self-focusing lenses according to the same interval angle, and a tightly-focused light beam with a high numerical aperture is formed and extends into the culture dish 7 to simultaneously manipulate a plurality of samples, so that the capture range is expanded.
Referring to fig. 2-3, in order to further optimize the above technical solution, N high na self-focusing lenses in the self-focusing lens array 5 are disposed corresponding to N microlens positions in the microlens array 4.
In order to further optimize the above technical solution, the sample 6 is a nearly spherical sample to be tested suspended in a culture dish, and comprises single cells, cell groups or particles with the maximum diameter of micron or nanometer.
To further improveOptimizing the technical scheme, the size specification of the self-focusing lens is
The length is 3.4 mm.
Example 2
A realization method of micro optical tweezers based on a self-focusing lens array comprises the following steps: a laser beam emitted by a laser 1 forms a parallel laser beam through an optical fiber 2 and a collimator 3, the parallel laser beam is incident on a micro-lens array 4 to generate N beams of focusing light, wherein N is greater than or equal to 3, the focusing light is immediately coupled into a self-focusing lens array 5 to form a tightly focused beam with a high numerical aperture, and the focusing beam extends into a culture solution of a culture dish 7 to grab a sample 6.
In order to further optimize the above technical solution, the N focused lights are respectively incident into N high numerical aperture self-focusing lenses of the self-focusing lens array 5.
In order to further optimize the above technical solution, the focused light is incident on the front working surface of the self-focusing lens array 5 at the focal plane of the micro lens array 4.
The above detailed description is provided for the micro optical tweezers device and method based on the self-focusing lens array, and the specific examples are applied herein to explain the principle and the implementation of the present invention, and the description of the above examples is only used to help understanding the method and the core idea of the present invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, there may be variations in the specific embodiments and the application scope, and in summary, the content of the present specification should not be construed as a limitation to the present invention.
In this document, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.
Claims (9)
1. The utility model provides a miniature optical tweezers device based on self-focusing lens array which characterized in that according to the light propagation direction in proper order: the device comprises a laser (1), an optical fiber (2), a collimator (3), a micro-lens array (4) and a self-focusing lens array (5), wherein a focusing light beam is formed and extends into a culture dish (7) to grab a sample (6); the sample (6) is placed in a liquid environment of a culture dish (7), the liquid environment comprising a culture liquid.
2. The micro optical tweezers device based on the self-focusing lens array according to claim 1, wherein the micro lens array (4) integrates N micro lenses, where N is greater than or equal to 3, and generates N focused beams, and the number of the focused beams is the same as the number of the high numerical aperture self-focusing lenses in the self-focusing lens array (4).
3. The micro optical tweezers device based on the self-focusing lens array according to claim 1, wherein the front working surface of the self-focusing lens array (5) is placed at the focal plane of the micro lens array (4).
4. The micro optical tweezers device based on the self-focusing lens array according to claim 1, wherein the self-focusing lens array (5) is formed by symmetrically combining N high numerical aperture self-focusing lenses according to the same interval angle, and the tightly focused light beam forming the high numerical aperture extends into the culture dish (7) to manipulate multiple samples simultaneously.
5. The micro optical tweezers device based on the self-focusing lens array according to claim 4, wherein the N high numerical aperture self-focusing lenses in the self-focusing lens array (5) are arranged corresponding to the N micro lens positions in the micro lens array (4).
6. The micro optical tweezers device based on the self-focusing lens array, as claimed in claim 1, wherein the sample (6) is a nearly spherical sample to be tested suspended in a petri dish, comprising single cells, cell clusters or particles with a maximum diameter of micro or nano scale.
7. A method for implementing micro optical tweezers based on self-focusing lens array, according to any one of claims 1 to 6, comprising:
a laser beam emitted by a laser (1) forms a parallel laser beam through an optical fiber (2) and a collimator (3), the parallel laser beam is incident on a micro-lens array (4) to generate N beams of focusing light, wherein N is greater than or equal to 3, the focusing light is coupled into a self-focusing lens array (5) immediately to form a tightly focused beam with a high numerical aperture, and the focusing beam extends into a culture solution of a culture dish (7) to grab a sample (6).
8. The method according to claim 7, wherein the N focused beams of light are respectively incident on N high numerical aperture self-focusing lenses of the self-focusing lens array (5).
9. The method for realizing micro optical tweezers based on the self-focusing lens array according to claim 7, wherein the focused light is incident on the front working surface of the self-focusing lens array (5) at the focal plane of the micro lens array (4).
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| CN202010056457.XA CN111061053A (en) | 2020-01-18 | 2020-01-18 | Micro optical tweezers device and method based on self-focusing lens array |
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| CN202010056457.XA CN111061053A (en) | 2020-01-18 | 2020-01-18 | Micro optical tweezers device and method based on self-focusing lens array |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114395463A (en) * | 2021-12-10 | 2022-04-26 | 广州大学 | CTC enrichment and release system based on micro-fluidic and low-light-level tweezers array and preparation method |
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| US20020034014A1 (en) * | 2000-07-31 | 2002-03-21 | Gretton Geoffrey B. | Microlens arrays having high focusing efficiency |
| US20060233490A1 (en) * | 2005-04-15 | 2006-10-19 | Yung-Shan Lin | Optical print head, method for enhancing light quantity thereof and optical printer |
| US20100019136A1 (en) * | 2006-07-26 | 2010-01-28 | Fabrice Merenda | Miniaturized optical tweezers based on high-na micro-mirrors |
| CN102445732A (en) * | 2012-01-05 | 2012-05-09 | 浙江大学 | Multi-beam optical tweezers based on planar optical waveguide |
| CN109188673A (en) * | 2018-09-14 | 2019-01-11 | 上海电力学院 | A kind of adjustable light forceps device |
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2020
- 2020-01-18 CN CN202010056457.XA patent/CN111061053A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020034014A1 (en) * | 2000-07-31 | 2002-03-21 | Gretton Geoffrey B. | Microlens arrays having high focusing efficiency |
| US20060233490A1 (en) * | 2005-04-15 | 2006-10-19 | Yung-Shan Lin | Optical print head, method for enhancing light quantity thereof and optical printer |
| US20100019136A1 (en) * | 2006-07-26 | 2010-01-28 | Fabrice Merenda | Miniaturized optical tweezers based on high-na micro-mirrors |
| CN102445732A (en) * | 2012-01-05 | 2012-05-09 | 浙江大学 | Multi-beam optical tweezers based on planar optical waveguide |
| CN109188673A (en) * | 2018-09-14 | 2019-01-11 | 上海电力学院 | A kind of adjustable light forceps device |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114395463A (en) * | 2021-12-10 | 2022-04-26 | 广州大学 | CTC enrichment and release system based on micro-fluidic and low-light-level tweezers array and preparation method |
| CN114395463B (en) * | 2021-12-10 | 2023-11-17 | 广州大学 | CTC enrichment and release system based on micro-fluidic and micro-optical tweezers arrays and preparation method |
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Application publication date: 20200424 |