CN111057645B - Microbial storage device for food decomposition and storage method thereof - Google Patents
Microbial storage device for food decomposition and storage method thereof Download PDFInfo
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- 235000013305 food Nutrition 0.000 title claims abstract description 52
- 230000000813 microbial effect Effects 0.000 title claims abstract description 38
- 238000000034 method Methods 0.000 title claims abstract description 11
- 238000000354 decomposition reaction Methods 0.000 title abstract description 11
- 244000005700 microbiome Species 0.000 claims abstract description 111
- 239000004094 surface-active agent Substances 0.000 claims abstract description 54
- 239000001963 growth medium Substances 0.000 claims abstract description 39
- 239000000203 mixture Substances 0.000 claims abstract description 25
- 239000002609 medium Substances 0.000 claims abstract description 20
- 235000015097 nutrients Nutrition 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 238000007789 sealing Methods 0.000 claims abstract description 7
- 239000010794 food waste Substances 0.000 claims description 34
- 244000063299 Bacillus subtilis Species 0.000 claims description 10
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 10
- 239000004382 Amylase Substances 0.000 claims description 9
- 102000013142 Amylases Human genes 0.000 claims description 9
- 108010065511 Amylases Proteins 0.000 claims description 9
- 108010059892 Cellulase Proteins 0.000 claims description 9
- 235000019418 amylase Nutrition 0.000 claims description 9
- 229940106157 cellulase Drugs 0.000 claims description 9
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 8
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims description 6
- 102000004882 Lipase Human genes 0.000 claims description 6
- 108090001060 Lipase Proteins 0.000 claims description 6
- 239000004367 Lipase Substances 0.000 claims description 6
- 108091005804 Peptidases Proteins 0.000 claims description 6
- 239000004365 Protease Substances 0.000 claims description 6
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 6
- 235000019421 lipase Nutrition 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 6
- 241000193388 Bacillus thuringiensis Species 0.000 claims description 5
- 229940097012 bacillus thuringiensis Drugs 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 241000193749 Bacillus coagulans Species 0.000 claims description 4
- 241000194108 Bacillus licheniformis Species 0.000 claims description 4
- 241000278457 Bacillus siamensis Species 0.000 claims description 4
- 229940054340 bacillus coagulans Drugs 0.000 claims description 4
- 241000194103 Bacillus pumilus Species 0.000 claims description 3
- 239000004519 grease Substances 0.000 claims description 3
- 238000009630 liquid culture Methods 0.000 claims description 3
- 239000004576 sand Substances 0.000 claims description 3
- 230000015556 catabolic process Effects 0.000 claims 1
- 238000006731 degradation reaction Methods 0.000 claims 1
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 230000002035 prolonged effect Effects 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 description 9
- 108090000790 Enzymes Proteins 0.000 description 9
- 229940088598 enzyme Drugs 0.000 description 9
- 230000000694 effects Effects 0.000 description 6
- 238000000855 fermentation Methods 0.000 description 6
- 230000004151 fermentation Effects 0.000 description 6
- 239000007788 liquid Substances 0.000 description 5
- 239000000047 product Substances 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 102000004157 Hydrolases Human genes 0.000 description 3
- 108090000604 Hydrolases Proteins 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 241000588986 Alcaligenes Species 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000005538 encapsulation Methods 0.000 description 2
- 238000011049 filling Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- FCBUKWWQSZQDDI-UHFFFAOYSA-N rhamnolipid Chemical compound CCCCCCCC(CC(O)=O)OC(=O)CC(CCCCCCC)OC1OC(C)C(O)C(O)C1OC1C(O)C(O)C(O)C(C)O1 FCBUKWWQSZQDDI-UHFFFAOYSA-N 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
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- 238000003756 stirring Methods 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- SFAYBQDGCKZKMH-UHFFFAOYSA-N BNCC Chemical compound BNCC SFAYBQDGCKZKMH-UHFFFAOYSA-N 0.000 description 1
- 241000835167 Bacillus safensis Species 0.000 description 1
- 241000193764 Brevibacillus brevis Species 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 102000004533 Endonucleases Human genes 0.000 description 1
- 108010042407 Endonucleases Proteins 0.000 description 1
- LLQPHQFNMLZJMP-UHFFFAOYSA-N Fentrazamide Chemical compound N1=NN(C=2C(=CC=CC=2)Cl)C(=O)N1C(=O)N(CC)C1CCCCC1 LLQPHQFNMLZJMP-UHFFFAOYSA-N 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 229920001503 Glucan Polymers 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 241000186610 Lactobacillus sp. Species 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 102000004139 alpha-Amylases Human genes 0.000 description 1
- 108090000637 alpha-Amylases Proteins 0.000 description 1
- 229940024171 alpha-amylase Drugs 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 239000000701 coagulant Substances 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- -1 etc.) Proteins 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 125000005456 glyceride group Chemical group 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000013586 microbial product Substances 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
Landscapes
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention discloses a microbial storage device for decomposing food, which comprises a storage container with a sealed inner space and a microbial mixture put in the storage container, wherein the microbial mixture comprises a culture medium of microbes for decomposing food and a surfactant, and the culture medium is used for supplying a nutrient medium for the growth and propagation of the microbes for decomposing food garbage. The invention also discloses a storage method of the microbial storage device for food decomposition, which comprises the steps of putting a culture medium containing the microbial for food decomposition and a surfactant into a container body, shaking for 15min at normal temperature, enabling the surfactant to form a surfactant film and wrap the outer surfaces of the culture medium, the microbes and water, and then sealing the open end of the container body by a container cover body, thus completing the storage of the microbial storage device for food decomposition. The microbial storage device for decomposing food has the beneficial effects that: the storage time of the microorganism is prolonged; and can provide sufficient nutrients for microorganisms.
Description
Technical Field
The invention belongs to the technical field of microbial storage, and particularly relates to a microbial storage device for food decomposition and a storage method thereof.
Background
The existing food waste is treated by landfill or incineration or processing into feed, fertilizer, etc. for recycling. Due to the problems of resource waste and environmental pollution, landfill or incineration of food waste is limited, and meanwhile, due to pathogenic problems, processing of food waste into feed is also gradually limited.
Therefore, in recent years, a food waste treatment apparatus of a liquid removal type is widely used in which microorganisms are introduced into a fermentation tank containing food waste, fermentation is performed after an appropriate temperature, humidity, air (oxygen) and the like are supplied, and then water and carbon dioxide are discharged.
Korean patent document KR101642321B1 discloses "Microorganism for decomposing a food waste a composition comprising the same and the eliminating method of a food waste using the same (a microbial composition for decomposing food waste consisting of bacillus subtilis DYG-B11 (Bacillus subtilis DYG-B11), bacillus subtilis DYG-B12 (Bacillus subtilis DYG-B12), lactobacillus DYGB13 (Lactobacillus sp. DYG-B13) and bacillus subtilis DYG-B14) and a method for decomposing food waste thereof)". The system of the microorganism composition contains Amylase (Amylase), cellulase (Cellulase), protease (Protease) and Lipase (Lipase). These microorganisms are sealed in a storage container for sale. The storage containers disclosed in this patent document are typically only for about 3 months of growth and propagation of these microorganisms. With increasing storage time, these microorganisms are susceptible to deterioration or the number of individuals is greatly reduced. If the time is too long, these microorganisms will not survive, resulting in loss of function of the microorganisms. However, since a long period of time is required from production-sales-use, and the storage time of microorganisms in the existing storage container is relatively short, a phenomenon that microorganisms cannot decompose food waste is liable to occur when the storage container is used. In particular, when microorganisms are produced into products and then exported, a long period of time (usually 2 to 3 months) is required from the completion of production of the products to the use thereof, and thus there is a problem that the microbial products cannot be exported because of the difficulty in storing the microorganisms.
Disclosure of Invention
The primary object of the present invention is to provide a microbial storage device for decomposing food, so as to solve the problem of short storage time of the existing storage device. A second object of the present invention is to provide a storage method of a microbial storage device for food decomposition.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
as a first aspect of the present invention, a microbial storage device for decomposing food, comprising a storage container having a sealed inner space and a microbial mixture placed in the storage container, the microbial mixture comprising a culture medium for a microbial for decomposing food and a surfactant, the surfactant being a hydrophilic surfactant, the culture medium being used for supplying a nutrient medium for growth and propagation of a microbial for decomposing food waste.
According to the invention, the storage container comprises a container body containing a microorganism mixture, wherein a container cover body is arranged on the container body, and the container cover body and the container body are connected in a sealing way.
According to the invention, the medium consists of a liquid medium, or,
the culture medium consists of a gel-like solid culture medium.
According to the invention, the surfactant comprises 20% to 35% of the total volume of the mixture of microorganisms. When the mixing ratio of the surfactant is less than 20%, there is a possibility that the microorganism may deteriorate beyond the shelf life. When the surfactant exceeds 35%, the surfactant is excessively wasted. The mixing proportion of the surfactant is preferably 20% -30%.
According to the invention, the microbial mixture further comprises Amylase (Amylase), cellulase (Cellulase), protease (Protease) and Lipase (Lipase). Amylase (Amylase) is an enzyme that breaks down starch, and Cellulase (Cellulase) is an enzyme that breaks down cellulose (cellulose). Food waste is composed of various organic matters such as carbohydrates, proteins, fats and the like, and the microorganism mixture can be decomposed and cleaned of the organic matters of the food waste through the enzyme activity as described above.
According to the invention, the microorganism mixture is used for decomposing food waste, and the microorganism is at least one of bacillus amyloliquefaciens (Bacillus amylo liquefaciens), bacillus siamensis (Bacillus siamensis), bacillus subtilis (Bacillus subtilis), bacillus pumilus (Brevibaccillus breris), bacillus thuringiensis (Bacillus thuringiensis), bacillus licheniformis (Bacillus Licheniformis), bacillus coagulans (Bacillus coagulans), bacillus sand (Bacillus safensis), bacillus desert (Bacillus inaquosorum) and the like. Such microorganisms for decomposing food waste may be used under aerobic conditions. Aerobic conditions refer to conditions in which air or oxygen is present.
Further, the microorganism further includes a microorganism for decomposing grease.
According to the present invention, the microorganism is mixed in a medium and is coated with the medium, and the outside of the microorganism medium is coated with a surfactant film, so that the microorganism and the medium are coated with the surfactant film. The surfactant film is also filled with water. With this structure, the microorganism of the present invention can survive with the nutrient components of the medium, and can survive in a stable state due to the encapsulation of the surfactant film.
As a second aspect of the present invention, a storage method of a microbial storage device for food decomposition is:
putting a culture medium containing microorganisms for decomposing food and a surfactant into a container body, shaking at normal temperature for about 15min to uniformly mix the culture medium and the surfactant, forming a surfactant film by the surfactant and wrapping the surfactant film outside the culture medium, the microorganisms and water, and sealing the open end of the container body by a container cover body to finish the storage of the microorganism storage device for decomposing food.
In use, microorganisms for decomposing food waste are inoculated onto a medium, and then a microorganism storage device for decomposing food containing microorganisms is put into a fermentation tank located inside a food waste treatment apparatus together with the food waste, and stirred inside the fermentation tank. After stirring, the food waste is fermented under the action of microorganisms to decompose the microorganisms.
The microbial storage device for decomposing food has the beneficial effects that:
1. microorganisms for decomposing food can be stored in the storage container for a long time, so that the sales and export of the microorganism storage device for decomposing food are easy;
2. when decomposing food waste by microorganisms, the microorganism storage device for decomposing food can provide sufficient nutrients for microorganisms, keep the microorganisms in a viable state, and decompose food waste, so that the utilization value of the microorganism for decomposing food can be increased.
Drawings
Fig. 1 is a schematic structural view of a microbial storage device for food decomposition according to the present invention.
FIG. 2 is a schematic view showing a state in which the microorganism of the present invention is coated with a surfactant film.
Description of the figure:
100. a storage container; 110. a container body; 120. a container cover; 200. a mixture of microorganisms; 210. a microorganism; 220. a culture medium; 230. a surfactant film; 240. and (3) water.
Detailed Description
The microbial storage device for food decomposition and the storage method thereof according to the present invention will be described in further detail with reference to the accompanying drawings.
Example 1
As shown in fig. 1, the microbial storage device for decomposing food according to the present invention comprises a storage container 100 having a sealed inner space, and a microbial mixture 200 placed in the storage container 100, wherein the microbial mixture 200 comprises a culture medium for decomposing food microorganisms and a surfactant, and the culture medium is used for supplying a nutrient medium for growth and propagation of the food waste decomposition microorganisms.
The storage container comprises a container body 110 containing a microorganism mixture, wherein a container cover 120 is arranged on the container body 110, and the container cover 120 and the container body 110 are connected in a sealing way.
The culture medium can be composed of a liquid culture medium or a gel-like solid culture medium.
It should be noted that a liquid medium or a jelly-like solid medium which can satisfy the growth and propagation needs of microorganisms may be used. The liquid medium of this example is C 6 H 12 O 6 0.2％，K 2 HPO 4 0.7％，KH 2 PO 4 0.3％，Na 3 C 6 H 5 O 7 0.05％，MgSO 4 ·7H 2 O 0.01％，(NH 4 ) 2 SO 4 0.1％，H 2 O100 mL. The gel-like solid medium of this example is a liquid medium containing a coagulant, such as agar, gelatin, or silica gel. Solid culture media are commonly used in microbiological separations, identification, enumeration, and strain preservation.
The surfactant is a hydrophilic surfactant (such as rhamnolipid or tween 80, etc.), and preferably accounts for 20% -35% of the total volume of the microorganism mixture (200). When the mixing ratio of the surfactant is less than 20%, there is a possibility that the microorganism may deteriorate beyond the shelf life. When the surfactant exceeds 35%, the surfactant is excessively wasted. The mixing proportion of the surfactant is preferably 20% -30%.
The microorganism is a microorganism which can be used for decomposing food and can be used under aerobic conditions, which means conditions in which air or oxygen is present. For example, bacillus amyloliquefaciens (available from the Meta Biotechnology development Co., ltd.) in the oil filling arm, bacillus siamensis (available from the Beijing North Nakataa Biotechnology Co., ltd.), bacillus subtilis, bacillus pumilus, bacillus thuringiensis, bacillus licheniformis (available from the Weifang Yi Hao Biotechnology Co., ltd.), bacillus coagulans (available from the Beijing North Nakataa Biotechnology Co., ltd.), bacillus sand (available from the Xueyang Kunlun Biotechnology Co., ltd.), and Bacillus desert (available from the Ji Nanfu Biotechnology Co., ltd.).
The microorganisms for decomposing food waste also include microorganisms for decomposing grease (for example, denitrifying alcaligenes, derived from CCTCC NO: M2011368).
The microbial mixture also includes amylase (e.g., one or more of alpha-amylase, 1, 4-alpha-D-glucan hydrolase, etc.) for decomposing foods such as vegetables, cellulase (e.g., beta-1, 4-glucan-4-glucan hydrolase, etc.), protease, and lipase (e.g., glyceride hydrolase, etc.) for decomposing vegetables, etc.
As shown in fig. 2, the microorganisms are mixed in and coated with a culture medium, and the outside of the microorganism culture medium is coated with a surfactant film, so that the microorganisms and the culture medium are coated with the surfactant film. The surfactant film is also filled with water. When the microorganism, the medium and the surfactant film are brought into the state as shown in fig. 2, the microorganism can survive with the nutrient components of the medium, and the microorganism can survive in a stable state due to the encapsulation of the surfactant film.
Therefore, with the microorganism storage device for decomposing food according to the present invention, the microorganism can remain viable after a user stores the microorganism for a period of time, and the food waste can be decomposed. Therefore, even if the microorganism storage means for decomposing food is exported to abroad, the microorganisms can survive for a long time, so that the microorganism storage means for decomposing food realizes export.
Example 2 test of survival or absence of microorganisms in a microorganism storage device for decomposing food and enzyme Activity test
In this example, 8 kinds of microorganisms were used as targets, and 8 kinds of microorganisms were placed on a medium, mixed with a surfactant, placed in a container body 110, and sealed with a container lid 20, and after a certain period of time, it was confirmed whether or not the microorganisms and enzymes survived. Wherein the microorganism, the culture medium and the surfactant are filled in the container body in a mixed state, and the volume of the surfactant is 20% -30% of the total volume (sum of the volumes of the surfactant and the culture medium). The surfactant of this example is rhamnolipid and the culture medium is C 6 H 12 O 6 0.2％，K 2 HPO 4 0.7％，KH 2 PO 4 0.3％，Na 3 C 6 H 5 O 7 0.05％，MgSO 4 ·7H 2 O 0.01％，(NH 4 ) 2 SO 4 0.1％，H 2 O 100mL。
The test results are shown in Table 1. The storage temperature of table 1 was room temperature and the storage time was measured in months at intervals as to whether the microorganism survived and the activity of the enzyme.
Table 1 8 survival of microorganisms and enzyme Activity
Wherein,
n1: bacillus amyloliquefaciens (Bacillus amylo liquefaciens) available from the human biotechnology development Co., ltd. In the oil filling arm
N2: siamese bacillus (product number BNCC 341566), purchased from Beijing North Innova Biotechnology institute
And N3: bacillus subtilis (ACCC 01031) for decomposing food waste
N4: brevibacillus brevis (ACCC 03387) for decomposing food waste
N5: bacillus thuringiensis (ACCC 01585) for decomposing food waste
N6: denitrifying alcaligenes (CCTCC NO: M2011368)
N7: amylase, an endonuclease, capable of randomly hydrolyzing a-1,4 glycosidic bond in starch, soluble dextrin and oligosaccharide, purchased from Zaozhuang Jinding biotechnology Co., ltd
N8: cellulase (CAS number: 9012-54-8), available from Hebei, inc. of Biotechnology Co., ltd
In table 1, "survival" means that there is little change in the number of individuals of the microorganism or little change in the activity of the enzyme, "change/decrease" means that the number of individuals of the microorganism is partially decreased or that a certain number of individuals of the microorganism is in a state of survival, or that the activity of the enzyme is decreased; "deformation" refers to a state in which viable microorganisms are almost absent. In the case of "change/decrease", it can be seen that the viable microorganisms can proliferate again although the number of individuals of the microorganisms is partially reduced, so that it can become "viable" after a certain period of time.
The results in Table 1 show that 8 microorganisms can be stored in a viable state for a minimum of 12 months at room temperature. When the microorganisms N5 and N6 are stored for 15 months, the microorganisms N1, N2, N3 and N4 are deformed, but the microorganisms N1, N2, N3 and N4 can survive for more than 15 months.
Therefore, even if the microorganism storage device for decomposing food is used after being stored for a certain period of time by a user, the microorganism can be kept alive, and the food waste can be decomposed, thereby improving the utility value of the microorganism product. Also, even if the food decomposing microorganism storage means for decomposing food is exported to abroad, microorganisms can survive for a long time, so that the food decomposing microorganism storage means realizes export.
The storage method of the microbial storage device for decomposing food comprises the following steps:
adding culture medium containing microorganism for decomposing food and surfactant into container body, shaking at room temperature for about 15min to mix culture medium and surfactant, and sealing the open end of container body with container cover to store microorganism for decomposing food.
In use, the microorganism storage device for decomposing foods containing microorganisms is put into a fermentation tank located in the food waste treatment apparatus together with the food waste, and stirred in the fermentation tank. After stirring, the food waste is fermented under the action of microorganisms to decompose the microorganisms.
In summary, the microorganism storage device for decomposing food according to the present invention can be used by a user after storing for a certain period of time, and the microorganisms can remain viable and decompose food waste. Therefore, even if the microorganism storage means for decomposing food is exported to abroad, the microorganisms can survive for a long time, so that the microorganism storage means for decomposing food realizes export.
The foregoing is merely a preferred embodiment of the invention, and it should be noted that modifications and enhancements can be made by those skilled in the art without departing from the principles of the present invention. Such modifications and variations are also considered to be a departure from the scope of the invention.
Claims (5)
1. A microbial storage device for decomposing food, which is characterized by comprising a storage container with a sealed inner space and a microbial mixture put in the storage container, wherein the microbial mixture comprises a culture medium of microbes for decomposing food and a surfactant, the surfactant is a hydrophilic surfactant, and the culture medium is used for supplying a nutrient medium for the growth and propagation of the microbes for decomposing food waste;
the storage container comprises a container body for containing a microorganism mixture, wherein a container cover body is arranged on the container body, and the container cover body is in sealing connection with the container body;
the culture medium consists of a liquid culture medium or a gel-like solid culture medium;
the liquid culture medium is C 6 H 12 O 6 0.2％，K 2 HPO 4 0.7％，KH 2 PO 4 0.3％，Na 3 C 6 H 5 O 7 0.05％，MgSO 4 ·7H 2 O 0.01％，(NH 4 ) 2 SO 4 0.1％，H 2 O 100mL；
The surfactant accounts for 20% -35% of the whole volume of the microorganism mixture;
the microorganisms are mixed in the culture medium and are wrapped by the culture medium, the surface of the culture medium is coated with the surfactant film, the microorganisms and the culture medium are wrapped by the surfactant film, and the surfactant film is filled with water.
2. The microbial storage device for food degradation according to claim 1, wherein the microbial blend further comprises amylase, cellulase, protease and lipase.
3. The microbial storage device for decomposing food according to claim 1, wherein the microbial mixture further comprises a microorganism for decomposing food waste, wherein the microorganism is at least one of bacillus amyloliquefaciens, bacillus siamensis, bacillus subtilis, bacillus pumilus, bacillus thuringiensis, bacillus licheniformis, bacillus coagulans, bacillus sand and bacillus desert.
4. The microbial storage device for decomposing food as defined in claim 3, wherein said microbes further include microbes for decomposing grease.
5. A storage method of a microbial storage device for decomposing food is characterized in that,
putting a culture medium containing microorganisms for decomposing food and a surfactant into a container body, shaking for 15min at normal temperature, uniformly mixing the culture medium and the surfactant, forming a surfactant film by the surfactant and wrapping the surfactant film outside the culture medium, the microorganisms and water, and sealing the open end of the container body by a container cover body to finish the storage of the microorganism storage device for decomposing food.
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| CN201911406410.5A CN111057645B (en) | 2019-12-31 | 2019-12-31 | Microbial storage device for food decomposition and storage method thereof |
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| CN201911406410.5A CN111057645B (en) | 2019-12-31 | 2019-12-31 | Microbial storage device for food decomposition and storage method thereof |
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| CN110283726A (en) * | 2019-07-03 | 2019-09-27 | 孝感宏翔生物医械技术有限公司 | A kind of liquid basal cell and microbiological treatment save reagent and preparation method thereof |
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