CN111020061A - Multiplex PCR primer group, kit and method for detecting HPV based on high-throughput sequencing - Google Patents
Multiplex PCR primer group, kit and method for detecting HPV based on high-throughput sequencing Download PDFInfo
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- CN111020061A CN111020061A CN201911409567.3A CN201911409567A CN111020061A CN 111020061 A CN111020061 A CN 111020061A CN 201911409567 A CN201911409567 A CN 201911409567A CN 111020061 A CN111020061 A CN 111020061A
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- primer
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/708—Specific hybridization probes for papilloma
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
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Abstract
Description
name (R) | Primer sequence (5 '→ 3') |
P5-01 | CAAGCAGAAGACGGCATACGAGATAACGTGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC |
P5-02 | CAAGCAGAAGACGGCATACGAGATAAACATCGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC |
P5-03 | CAAGCAGAAGACGGCATACGAGATATGCCTAAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC |
P5-04 | CAAGCAGAAGACGGCATACGAGATAGTGGTCAGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC |
P5-05 | CAAGCAGAAGACGGCATACGAGATACCACTGTGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC |
P5-06 | CAAGCAGAAGACGGCATACGAGATACATTGGCGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC |
P5-07 | CAAGCAGAAGACGGCATACGAGATCAGATCTGGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC |
P5-08 | CAAGCAGAAGACGGCATACGAGATCATCAAGTGTGACTGGAGTTCAGACGTGTGCTCTTCCGATC |
P7-01 | AATGATACGGCGACCACCGAGATCTACACAGATCGCAACACTCTTTCCCTACACGACGCTCTTCCGATC |
P7-02 | AATGATACGGCGACCACCGAGATCTACACAGCAGGAAACACTCTTTCCCTACACGACGCTCTTCCGATC |
P7-03 | AATGATACGGCGACCACCGAGATCTACACAGTCACTAACACTCTTTCCCTACACGACGCTCTTCCGATC |
P7-04 | AATGATACGGCGACCACCGAGATCTACACATCCTGTAACACTCTTTCCCTACACGACGCTCTTCCGATC |
P7-05 | AATGATACGGCGACCACCGAGATCTACACATTGAGGAACACTCTTTCCCTACACGACGCTCTTCCGATC |
P7-06 | AATGATACGGCGACCACCGAGATCTACACCAACCACAACACTCTTTCCCTACACGACGCTCTTCCGATC |
P7-07 | AATGATACGGCGACCACCGAGATCTACACGACTAGTAACACTCTTTCCCTACACGACGCTCTTCCGATC |
P7-08 | AATGATACGGCGACCACCGAGATCTACACCAATGGAAACACTCTTTCCCTACACGACGCTCTTCCGATC |
P7-09 | AATGATACGGCGACCACCGAGATCTACACCACTTCGAACACTCTTTCCCTACACGACGCTCTTCCGATC |
P7-10 | AATGATACGGCGACCACCGAGATCTACACCAGCGTTAACACTCTTTCCCTACACGACGCTCTTCCGATC |
P7-11 | AATGATACGGCGACCACCGAGATCTACACCATACCAAACACTCTTTCCCTACACGACGCTCTTCCGATC |
P7-12 | AATGATACGGCGACCACCGAGATCTACACCCAGTTCAACACTCTTTCCCTACACGACGCTCTTCCGATC |
reagent | Final concentration |
PCR Mix | 0.8~1.2× |
Primer library | 0.2~5μM |
Any P5 joint | 0.02~0.5μM |
Any P7 joint | 0.02~0.5μM |
DNA | Make up to 20. mu.L |
reagent | Final concentration |
PCR Mix | 1× |
Primer library | 0.5μM |
Any P5 joint | 0.4μM |
Any P7 joint | 0.4μM |
DNA | Make up to 20. mu.L |
reagent | Final concentration |
PCR Mix | 1× |
Primer library | 0.5μM |
Any P5 joint | 0.4μM |
Any P7 joint | 0.4μM |
DNA | Make up to 20. mu.L |
Claims (8)
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CN201911409567.3A CN111020061B (en) | 2019-12-31 | 2019-12-31 | Multiplex PCR primer group, kit and method for detecting HPV based on high-throughput sequencing |
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CN201911409567.3A CN111020061B (en) | 2019-12-31 | 2019-12-31 | Multiplex PCR primer group, kit and method for detecting HPV based on high-throughput sequencing |
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CN111020061A true CN111020061A (en) | 2020-04-17 |
CN111020061B CN111020061B (en) | 2021-01-05 |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111455114A (en) * | 2020-05-22 | 2020-07-28 | 深圳华大智造科技有限公司 | High-flux detection kit for SARS-CoV-2 |
CN112029842A (en) * | 2020-08-31 | 2020-12-04 | 深圳市血液中心(深圳市输血医学研究所) | Kit and method for ABO blood type genotyping based on high-throughput sequencing |
CN112251501A (en) * | 2020-10-28 | 2021-01-22 | 深圳人体密码基因科技有限公司 | Reference gene set and screening method thereof, universal primer group, kit, reaction system and application |
CN112458195A (en) * | 2020-12-28 | 2021-03-09 | 广州迈景基因医学科技有限公司 | Multiplex PCR primer set, kit and method for detecting sexually transmitted pathogens based on high-throughput sequencing |
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CN104561372A (en) * | 2014-12-11 | 2015-04-29 | 南京普东兴生物科技有限公司 | Combined primer for amplification and typing of human papilloma virogenes and application of combined primer |
CN107988372A (en) * | 2018-01-03 | 2018-05-04 | 杭州联川基因诊断技术有限公司 | A kind of kit and its detection method for detecting susceptibility gene of colorectal cancer mutation |
CN108728578A (en) * | 2018-06-12 | 2018-11-02 | 苏州国科闻普生物科技有限公司 | Detect the method and kit of a variety of high-risk human mammilla papillomavirus and its hypotype simultaneously in single tube reaction |
CN109517885A (en) * | 2018-12-27 | 2019-03-26 | 北京迈基诺基因科技股份有限公司 | A kind of method and its primer special group based on high-flux sequence detection mouse DNA TCR beta chain immune group library |
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111455114A (en) * | 2020-05-22 | 2020-07-28 | 深圳华大智造科技有限公司 | High-flux detection kit for SARS-CoV-2 |
CN111455114B (en) * | 2020-05-22 | 2023-09-05 | 深圳华大智造科技股份有限公司 | High flux detection kit for SARS-CoV-2 |
CN112029842A (en) * | 2020-08-31 | 2020-12-04 | 深圳市血液中心(深圳市输血医学研究所) | Kit and method for ABO blood type genotyping based on high-throughput sequencing |
CN112029842B (en) * | 2020-08-31 | 2021-04-27 | 深圳市血液中心(深圳市输血医学研究所) | Kit and method for ABO blood type genotyping based on high-throughput sequencing |
CN112251501A (en) * | 2020-10-28 | 2021-01-22 | 深圳人体密码基因科技有限公司 | Reference gene set and screening method thereof, universal primer group, kit, reaction system and application |
CN112458195A (en) * | 2020-12-28 | 2021-03-09 | 广州迈景基因医学科技有限公司 | Multiplex PCR primer set, kit and method for detecting sexually transmitted pathogens based on high-throughput sequencing |
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Inventor after: Cai Xingsheng Inventor after: Wang Chunlin Inventor after: Li Mengzhen Inventor after: Deng Yangyang Inventor after: Ma Zhihai Inventor after: Qian Dongyuan Inventor after: Liu Yasi Inventor after: Yue Xiaoyan Inventor after: Yang Dongcheng Inventor before: Cai Xingsheng Inventor before: Li Mengzhen Inventor before: Deng Yangyang Inventor before: Wang Chunlin Inventor before: Ma Zhihai Inventor before: Qian Dongyuan Inventor before: Liu Yasi Inventor before: Yue Xiaoyan Inventor before: Yang Dongcheng |
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