CN111015862A - Method for dyeing bamboo and wood by using biological pigment - Google Patents
Method for dyeing bamboo and wood by using biological pigment Download PDFInfo
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- CN111015862A CN111015862A CN201911389838.3A CN201911389838A CN111015862A CN 111015862 A CN111015862 A CN 111015862A CN 201911389838 A CN201911389838 A CN 201911389838A CN 111015862 A CN111015862 A CN 111015862A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B27—WORKING OR PRESERVING WOOD OR SIMILAR MATERIAL; NAILING OR STAPLING MACHINES IN GENERAL
- B27K—PROCESSES, APPARATUS OR SELECTION OF SUBSTANCES FOR IMPREGNATING, STAINING, DYEING, BLEACHING OF WOOD OR SIMILAR MATERIALS, OR TREATING OF WOOD OR SIMILAR MATERIALS WITH PERMEANT LIQUIDS, NOT OTHERWISE PROVIDED FOR; CHEMICAL OR PHYSICAL TREATMENT OF CORK, CANE, REED, STRAW OR SIMILAR MATERIALS
- B27K5/00—Treating of wood not provided for in groups B27K1/00, B27K3/00
- B27K5/02—Staining or dyeing wood; Bleaching wood
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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Abstract
The invention discloses a method for dyeing bamboo and wood by regulating and controlling the growth of chromobacterium and pigment secretion. The process comprises 1) cultivating color-changing fungi into dyeing fungi solution; 2) soaking the bamboo wood in a dyeing fungus solution for inoculation; 3) controlling the growth speed and pigment secretion of color-changing fungi on different surfaces of the bamboo and wood; 4) sterilizing the dyed bamboo and wood; 5) maintaining and balancing to obtain the novel biological pigment dyed bamboo wood. The method for dyeing the bamboo and wood by using the biological pigment provided by the invention utilizes the excellent biocompatibility and anti-oxidation biological characteristics of the bamboo and wood color-changing fungi, has the advantages of environmental protection, environmental friendliness and the like, can be applied to dyeing of bamboo and wood unit products, and has a wide development prospect.
Description
Technical Field
The invention relates to a dyeing method for dyeing bamboo and wood by using biological pigment, belonging to the technical field of biotechnology and biological wood dyeing.
Background
The color is used as the external characteristic of bamboo and wood products, and is an important factor for enriching the decorative performance of bamboo and wood and improving the added value of bamboo and wood. In order to improve the decoration effect and the product value of the bamboo and wood products, the bamboo and wood products are dyed by adopting a proper method, and the method is an important technical means for improving the use value of the bamboo and wood and meeting the requirements of people on color diversity. The bamboo and wood dyeing process mainly comprises a constant-temperature impregnation method, a vacuum impregnation method, a cold-hot alternating method and the like. However, the conventional bamboo and wood dyeing process has the advantages of single product function and narrow application range, causes environmental pollution and harm to human health, and limits the industrial development of bamboo and wood dyeing.
Because the bamboo wood contains nutrient substances such as saccharides, starch and the like, the bamboo wood is suitable for fungus growth under warm and humid conditions, is easy to generate biological discoloration, and generates black, blue, brown, green, cyan and other colors. At present, most of researches on microorganisms and bamboo wood color are mainly for prevention and treatment, and the researches focus on inhibiting decay and color change of bamboo wood by utilizing the interspecies relationship of microorganisms and less research on modification treatment of bamboo wood by utilizing the microorganisms and products thereof. The method is characterized in that from the viewpoint of subjectively utilizing color-changing fungi, a dyeing fungus solution is prepared and inoculated on the surface of the bamboo wood, and the growth speed and pigment secretion of the bamboo wood are controlled by the anti-oxidation biological characteristics of the color-changing fungi, so that the novel biological pigment dyeing bamboo wood is obtained.
Disclosure of Invention
The invention provides a method for dyeing bamboo and wood by regulating the growth and pigment secretion of chromotropic fungi, which aims to prepare a dyeing fungus solution, inoculate the dyeing fungus solution on bamboo and wood, control the growth speed and the pigment secretion of chromotropic fungi on different surfaces of the bamboo and wood by inducing growth and secretion, and obtain a novel biological pigment dyeing bamboo and wood after sterilization treatment and maintenance balance. The novel method for dyeing the bamboo and wood by using the biological pigment is a sustainable, green and environment-friendly novel method for dyeing the bamboo and wood, and can be applied to dyeing and decoration of bamboo and wood products such as veneers, bamboo chips and the like.
The technical scheme of the invention comprises the following process steps:
1) the preparation formula comprises 30g/L of glucose, 3g/L of peptone and phosphorusDipotassium hydrogen acid 1.5g/L, magnesium sulfate (MgSO)4·7H2O)0.5g/L, ferrous sulfate 0.01g/L, distilled water, liquid medium with pH of 7.0, sterilizing the liquid medium in an autoclave at 121 deg.C under 0.11 MPa. Common color-changing fungi (i.e., the Lasiodipia theobromae, the Fusarium cubeba, the Trichoderma viride, the Paecilomyces lilacinus and the Fusarium sambucinum) in bamboo and wood are inoculated into a PDA culture medium by using a sterile puncher with the diameter of 10-15 mm, and are cultured for 5-10 days in a mold culture box with the temperature of 25-35 ℃ and the humidity of 75-90%. Washing spores generated on colonies in a PDA culture medium with a small amount of sterilized water, inoculating the spores to 10ml of PDB culture medium, preparing bacterial suspension, culturing at 30 ℃ for 24h, and then inoculating the bacterial suspension to a liquid culture medium according to the ratio of 1: 50-1: 100. Then, the strain is cultured in vitro under shaking at 150rpm and 26 ℃ for 3d, and is expanded to be a staining fungus solution.
2) Placing wood blocks or bamboo blocks with thickness of 5-30 mm and length (width) of more than or equal to 40 into an autoclave for sterilization at 121 ℃ under the pressure of 0.11MPa, then soaking in a dyeing fungus solution for inoculation, standing overnight at 25 ℃, and taking out;
3) placing the inoculated bamboo wood in a constant temperature and humidity box, culturing at the temperature of 25-35 ℃ and the humidity of 75-90%, coating the surface of the bamboo wood with allochroic fungi, propagating and growing in a proper growing environment, and secreting pigments. Passing through 0-15 mmol/L H2O2Or KClO4Inducing the growth and secretion of the color-changing fungi by the solution, and controlling the growth speed and the pigment secretion amount of the color-changing fungi on different surfaces of the bamboo and wood materials;
4) taking out the dyed bamboo and wood after the allochroic fungi are infected, removing hyphae on the surface of the bamboo and wood, putting the bamboo and wood into an autoclave, sterilizing at the temperature of 121 ℃ under the pressure of 0.11MPa, and taking out after sterilizing for 30 min;
5) and (3) carrying out damp-heat balance on the sterilized dyed bamboo and wood in an aseptic constant-temperature and constant-humidity box for more than 48 hours under the conditions that the temperature is 20-25 ℃ and the humidity is 8-14%, so that the water content of the dyed bamboo and wood is controlled to be 5-8%, and obtaining the novel biological pigment dyed bamboo and wood.
Detailed Description
Example 1: the color-changing fungus is Fusarium rubrum, the length, width and thickness of the wood block are respectively 500mm, 400mm and 10mm, and the wood block passes through 10mmol/L H2O2Growth and secretion of solution-induced color-changing fungi
The preparation formula of the step 1) comprises 30g/L of glucose, 3g/L of peptone, 1.5g/L of dipotassium hydrogen phosphate and magnesium sulfate (MgSO)4·7H2O)0.5g/L, ferrous sulfate 0.01g/L, distilled water, a liquid medium having a pH of 7.0, and sterilizing the liquid medium in an autoclave at a temperature of 121 ℃ and a pressure of 0.11 MPa. Fusarium cubilose (Gibberellabacaccata) strain was inoculated into PDA medium with a sterile punch of 10mm diameter and cultured in a mold incubator at a temperature of 27 ℃ and a humidity of 80% for 7 days. Spores generated on colonies in the PDA culture medium are washed by a small amount of sterilized water, and are inoculated into 10ml of PDB culture medium to prepare a bacterial suspension, and the bacterial suspension is inoculated into a liquid culture medium according to the ratio of 1:100 after being cultured for 24 hours at 30 ℃. Then carrying out shake culture in vitro for 3d at 26 ℃ and 150rpm, and carrying out amplification culture to obtain a staining fungus solution;
step 2) putting wood blocks with the length, width and thickness of 500mm, 400mm and 10mm into an autoclave for sterilization at the temperature of 121 ℃ and under the pressure of 0.11MPa, then soaking the wood blocks into a dyeing fungus solution for inoculation, standing the wood blocks at the temperature of 25 ℃ overnight, and taking the wood blocks out;
and 3) placing the inoculated bamboo wood in a constant temperature and humidity box, culturing at the temperature of 27 ℃ and the humidity of 80%, coating the surface of the bamboo wood with allochroic fungi, carrying out propagation and growth in a proper growth environment, and secreting pigments. By 10mmol/LH2O2Inducing the growth and secretion of the color-changing fungi by the solution, and controlling the growth speed and the pigment secretion amount of the color-changing fungi on different surfaces of the bamboo and wood materials;
step 4) taking out the dyed bamboo and wood after the allochroic fungi are infected, removing hyphae on the surface of the bamboo and wood, putting the bamboo and wood into an autoclave, sterilizing at the temperature of 121 ℃ and under the pressure of 0.11MPa, and taking out after sterilizing for 30 min;
and 5) carrying out damp-heat balance on the sterilized dyed bamboo and wood in a sterile constant-temperature and constant-humidity box for more than 48 hours under the conditions of 25 ℃ and 12% of humidity, so that the water content of the dyed bamboo and wood is controlled at 8%, and obtaining the novel biological pigment dyed bamboo and wood.
Example 2: the color-changing fungus is Coccodiopsis cacao, the length, width and thickness of bamboo pieces are respectively 800mm, 400mm and 5mm, and the bamboo pieces are subjected to KClO of 10mmol/L4Growth and secretion of solution-induced color-changing fungi
The preparation formula of the step 1) comprises 30g/L of glucose, 3g/L of peptone, 1.5g/L of dipotassium hydrogen phosphate and magnesium sulfate (MgSO)4·7H2O)0.5g/L, ferrous sulfate 0.01g/L, distilled water, a liquid medium having a pH of 7.0, and sterilizing the liquid medium in an autoclave at a temperature of 121 ℃ and a pressure of 0.11 MPa. A strain of Coccomydia cacao (Lasiodipia theobroma) was inoculated into PDA medium using a sterile punch having a diameter of 10mm, and cultured in a mold incubator at a temperature of 27 ℃ and a humidity of 80% for 7 days. Spores generated on colonies in the PDA culture medium are washed by a small amount of sterilized water, and are inoculated into 10ml of PDB culture medium to prepare a bacterial suspension, and the bacterial suspension is inoculated into a liquid culture medium according to the ratio of 1:100 after being cultured for 24 hours at 30 ℃. Then carrying out shake culture in vitro for 3d at 26 ℃ and 150rpm, and carrying out amplification culture to obtain a staining fungus solution;
step 2) placing bamboo blocks with the length, width and thickness of 800mm, 400mm and 5mm into an autoclave for sterilization at the temperature of 121 ℃ and under the pressure of 0.11MPa, then soaking the bamboo blocks into a dyeing fungus solution for inoculation, standing the bamboo blocks at the temperature of 25 ℃ overnight, and taking out the bamboo blocks;
and 3) placing the inoculated bamboo wood in a constant temperature and humidity box, culturing at the temperature of 27 ℃ and the humidity of 80%, coating the surface of the bamboo wood with allochroic fungi, carrying out propagation and growth in a proper growth environment, and secreting pigments. Through 10mmol/L KClO4Inducing the growth and secretion of the color-changing fungi by the solution, and controlling the growth speed and the pigment secretion amount of the color-changing fungi on different surfaces of the bamboo and wood materials;
step 4) taking out the dyed bamboo and wood after the allochroic fungi are infected, removing hyphae on the surface of the bamboo and wood, putting the bamboo and wood into an autoclave, sterilizing at the temperature of 121 ℃ and under the pressure of 0.11MPa, and taking out after sterilizing for 30 min;
and 5) carrying out damp-heat balance on the sterilized dyed bamboo and wood in a sterile constant-temperature and constant-humidity box for more than 48 hours under the conditions of 25 ℃ and 12% of humidity, so that the water content of the dyed bamboo and wood is controlled at 8%, and obtaining the novel biological pigment dyed bamboo and wood.
Example 3: the color-changing fungus is Paecilomyces lilacinus, the length, width and thickness of the bamboo pieces are respectively 800mm, 400mm and 5mm, and the color-changing fungus is obtained by passing 10mmol/L KClO4Growth and secretion of solution-induced color-changing fungi
The preparation formula of the step 1) comprises 30g/L of glucose, 3g/L of peptone, 1.5g/L of dipotassium hydrogen phosphate and magnesium sulfate (MgSO)4·7H2O)0.5g/L, ferrous sulfate 0.01g/L, distilled water, a liquid medium having a pH of 7.0, and sterilizing the liquid medium in an autoclave at a temperature of 121 ℃ and a pressure of 0.11 MPa. Paecilomyces lilacinus (Paecilomyces lilacinus) strain was inoculated into PDA medium with a sterile punch having a diameter of 10mm, and cultured in a mold incubator at a temperature of 27 ℃ and a humidity of 80% for 7 days. Spores generated on colonies in the PDA culture medium are washed by a small amount of sterilized water, and are inoculated into 10ml of PDB culture medium to prepare a bacterial suspension, and the bacterial suspension is inoculated into a liquid culture medium according to the ratio of 1:100 after being cultured for 24 hours at 30 ℃. Then carrying out shake culture in vitro for 3d at 26 ℃ and 150rpm, and carrying out amplification culture to obtain a staining fungus solution;
step 2) placing bamboo blocks with the length, width and thickness of 800mm, 400mm and 5mm into an autoclave for sterilization at the temperature of 121 ℃ and under the pressure of 0.11MPa, then soaking the bamboo blocks into a dyeing fungus solution for inoculation, standing the bamboo blocks at the temperature of 25 ℃ overnight, and taking out the bamboo blocks;
and 3) placing the inoculated bamboo wood in a constant temperature and humidity box, culturing at the temperature of 27 ℃ and the humidity of 80%, coating the surface of the bamboo wood with allochroic fungi, carrying out propagation and growth in a proper growth environment, and secreting pigments. Passing through 10mmol/LKCLO4Growth and secretion of solution-induced color-changing fungiControlling the growth speed and pigment secretion of the color-changing fungi on different surfaces of the bamboo and wood materials;
step 4) taking out the dyed bamboo and wood after the allochroic fungi are infected, removing hyphae on the surface of the bamboo and wood, putting the bamboo and wood into an autoclave, sterilizing at the temperature of 121 ℃ and under the pressure of 0.11MPa, and taking out after sterilizing for 30 min;
and 5) carrying out damp-heat balance on the sterilized dyed bamboo and wood in a sterile constant-temperature and constant-humidity box for more than 48 hours under the conditions of 25 ℃ and 12% of humidity, so that the water content of the dyed bamboo and wood is controlled at 8%, and obtaining the novel biological pigment dyed bamboo and wood.
Claims (8)
1. A method for dyeing bamboo wood by biological pigment is characterized by comprising the following steps: the method for dyeing the bamboo and wood by adopting the biological pigment is characterized in that bamboo and wood discoloring fungi with excellent biocompatibility are adopted to carry out biological infection dyeing on the bamboo and wood.
2. A method for dyeing bamboo wood by biological pigment is characterized by comprising the following process steps:
1) preparing a dyeing fungus solution: carrying out in-vitro oscillation amplification culture on common color-changing fungi in bamboo and wood by adopting a liquid culture medium to obtain a dyeing fungus solution, wherein the ratio of the fungus solution is 50: 1-100: 1;
2) bamboo wood inoculation: soaking bamboo wood in a dyeing fungus solution for inoculation, standing overnight, and taking out;
3) growth and secretion of color-changing fungi: placing the inoculated bamboo wood in a constant temperature and humidity box for culture, wrapping the bamboo wood with color-changing fungi for reproduction and growth, secreting pigments, and controlling the growth speed and the pigment secretion of the color-changing fungi on different surfaces of the bamboo wood through induced growth and secretion;
4) and (3) sterilization treatment: taking out the dyed bamboo and wood after the discoloring fungi are infected, removing hyphae on the surface of the bamboo and wood, and putting the bamboo and wood into an autoclave for sterilization treatment;
5) maintaining and balancing: and (3) carrying out humidity and heat balance on the sterilized dyed bamboo wood in an aseptic constant temperature and humidity box until the water content is 5-8%, thus obtaining the novel biological pigment dyed bamboo wood.
3. A method of bio-pigment dyeing of bamboo and wood according to claim 2, characterized in that: the formula of the liquid culture medium in the step 1) is 30g/L of glucose, 3g/L of peptone, 1.5g/L of dipotassium hydrogen phosphate and magnesium sulfate (MgSO)4·7H2O)0.5g/L, ferrous sulfate 0.01g/L, distilled water, and adjusting the pH of the liquid culture medium to 7.0.
4. A method of bio-pigment dyeing of bamboo and wood according to claim 2, characterized in that: the common color-changing fungus in the step 1) is one of cacao-shaped diplodia theobromae (Lasiodipdia theobromae), fusarium graminearum (Gibberellabacacta), Trichoderma viride (Trichoderma viride), Paecilomyces lilacinus (Paecilomyces lilacinus) and fusarium sambucinum.
5. A method of bio-pigment dyeing of bamboo and wood according to claim 2, characterized in that: and in the step 2), the thickness of the bamboo wood is 5-30 mm, and the length (width) and the thickness are more than or equal to 40.
6. A method of bio-pigment dyeing of bamboo and wood according to claim 2, characterized in that: the induced growth and secretion in the step 3) adopts 0-15 mmol/L H2O2Or KClO4And (3) solution.
7. A method of bio-pigment dyeing of bamboo and wood according to claim 2, characterized in that: and 4) the sterilization treatment process is carried out at the temperature of 121 ℃ and the pressure of 0.11 MPa.
8. The method of bio-pigment dyeing of bamboo wood according to any one of claims 1 to 7, characterized in that: the method is characterized in that allochroic bacteria in bamboo and wood are reversely utilized and inoculated on the surface of the bamboo and wood for propagation culture, and the growth speed and the pigment secretion are controlled by inducing the allochroic bacteria through the anti-oxidation biological characteristics of the allochroic bacteria, so that the method for dyeing the bamboo and wood by the biological pigment is prepared.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0413909B1 (en) * | 1989-08-23 | 1994-02-02 | DESOWAG Materialschutz GmbH | Agent or concentrate for the preservation of wood or wooden objects |
| CN103659976A (en) * | 2013-09-05 | 2014-03-26 | 河南科技大学 | Method for biologically dyeing wood through fistulina hepatica |
| CN109079947A (en) * | 2018-08-06 | 2018-12-25 | 北京林业大学 | A kind of stained wood for the method and preparation that wood staining is handled |
-
2019
- 2019-12-30 CN CN201911389838.3A patent/CN111015862A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0413909B1 (en) * | 1989-08-23 | 1994-02-02 | DESOWAG Materialschutz GmbH | Agent or concentrate for the preservation of wood or wooden objects |
| CN103659976A (en) * | 2013-09-05 | 2014-03-26 | 河南科技大学 | Method for biologically dyeing wood through fistulina hepatica |
| CN109079947A (en) * | 2018-08-06 | 2018-12-25 | 北京林业大学 | A kind of stained wood for the method and preparation that wood staining is handled |
Non-Patent Citations (3)
| Title |
|---|
| 俞晓艳等: "《景观花卉实用技术》", 31 December 2007, 银川：宁夏人民出版社 * |
| 蔡晶晶等: "《药用微生物技术实训》", 31 July 2013, 南京：东南大学出版社 * |
| 郑小波: "《疫霉菌及其研究技术》", 31 March 1997, 北京：中国农业出版社 * |
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Application publication date: 20200417 |