CN111007660A

CN111007660A - Phase contrast microscope multi-mode ultrathin light source device and using method thereof

Info

Publication number: CN111007660A
Application number: CN201911356154.3A
Authority: CN
Inventors: 范永涛; 罗海清; 孙彦华; 李�杰
Original assignee: Shanghai Guanna Intelligent Technology Co Ltd
Current assignee: Shanghai Guanna Intelligent Technology Co ltd
Priority date: 2019-12-25
Filing date: 2019-12-25
Publication date: 2020-04-14
Anticipated expiration: 2039-12-25
Also published as: CN111007660B

Abstract

The invention discloses a multi-mode ultrathin light source device of a phase contrast microscope and a using method thereof. One end of the transparent light guide ring is sleeved on the inner side and the outer side of the middle annular array of the LED array, the other end of the transparent light guide ring is connected with the light-diffusing plate, the light-diffusing plate performs homogenization treatment on light emitted by the LED lamp, and the glass is tightly attached to the outer surface of the light-diffusing plate and used for fixing the light-diffusing plate and the transparent light guide ring. The light source device can realize the switching of two imaging modes of phase contrast illumination and bright field illumination through circuit switching, has the total height smaller than 20mm, and can be very conveniently applied to a microscope with higher requirement on the automation degree.

Description

Phase contrast microscope multi-mode ultrathin light source device and using method thereof

Technical Field

The present invention relates to a component of a phase contrast microscope, particularly to a light source device of a phase contrast microscope and a use method thereof.

Background

Phase contrast microscopy is the most commonly used microscope in biological studies, which often involves the observation of thin, transparent samples, such as cells, which are often difficult to directly see under ordinary light microscopy because they are too thin and transparent. When we observe cells in middle school biology experiments, we often use a specific dye to stain the cells, or directly observe colored cells, such as onion epidermal cells. However, in actual biological research, a method for directly observing cells without treatment is needed, and the phase contrast microscope well solves the problem. After the illumination light passes through the transparent sample, the imaging cannot be directly observed due to small light intensity change, but due to the components and the density of the sample, different phase shifts are generated when the light passes through, and the phase contrast microscope essentially performs imaging by using the phase shift. The good illumination condition is an indispensable condition for biological research, in order to provide the good illumination condition, the light source devices of the existing phase contrast microscopes are complex and have large volume, and in addition, the light path needs to be processed when switching between phase contrast imaging and bright field imaging, so the operation is very inconvenient.

In the prior art, the invention patent of "light source device of fluorescence microscope" (patent number of invention: CN 105445919A) proposes a light source device of fluorescence microscope, which is in a shape of a circular rotating disk, more than two LEDs are distributed on the rotating disk, and the switching between different LEDs is realized by rotating the rotating disk. The invention discloses an integrated light source device for a microscope objective array (patent number: CN 110244443A), and provides an integrated light source device for a microscope objective array, which is more uniform. The above patents have certain advantages, but there are some disadvantages: the invention discloses a light source device of a fluorescence microscope, which adds a wiring plate and a wiring seat for an LED turntable, increases the volume of the whole light source device, and can not realize the switching between different modes; the invention discloses an integrated light source device for a micro-array, which comprises a light source, Mie scattering devices, an optical cavity and a condenser, wherein optical cement is used for connecting the three devices, the stability of the device is poor, the volume of the integrated light source device is large, and the switching among different modes cannot be realized. The invention patent 'LED array-based multimode microscopic imaging system and method' (invention patent No. CN 104765138A) discloses a multimode microscopic imaging system of an LED array, which takes the LED array as a light source of a microscopic system to generate controllable multi-angle illuminating light and controllable illuminating aperture so as to realize bright field imaging, dark field imaging and differential phase contrast imaging. However, when dark field and differential phase contrast imaging are realized, because the light of the LED is scattered to the periphery instead of a single direction, it is difficult to form a poor light path as shown in the figure, resulting in poor phase contrast effect and difficulty in achieving good imaging requirements.

Disclosure of Invention

The present invention is directed to a multimode ultra-thin light source device of a phase contrast microscope and a method for using the same, which solves the above-mentioned shortcomings of the conventional light source device. The light source device has the advantages of simple structure, ultra-thin and multi-mode switching.

In order to achieve the above object, the technical solution of the present invention is as follows:

a multi-mode ultrathin light source device of a phase contrast microscope comprises an LED base, wherein an LED array is arranged on the LED base; the LED array comprises an inner circular array, a middle circular array surrounds the inner circular array, and an outer circular array surrounds the middle circular array; annular light shading plates are fixed on the inner side and the outer side of the middle annular array; the lower part is provided with a light-diffusing plate, and the lower part of the light-diffusing plate is provided with glass.

In a further improvement, the annular light shading plate is a transparent light guide ring, and black paint is sprayed on the inner wall and the outer wall of the transparent light guide ring (3).

In a further improvement, the LED array is composed of a series of concentric LED lamps; the inner circular array, the middle annular array and the outer annular array are all concentrically arranged.

In a further improvement, the middle ring array is a third ring array of LED arrays; transparent light guide rings are embedded on the inner side and the outer side of the third ring array.

In a further improvement, the light-diffusing plate is closely connected with the lower end of the transparent light-guiding ring, so that light is prevented from diffusing outwards. The light-diffusing plate homogenizes light emitted by the LED lamp.

In a further improvement, the glass is closely adhered to the light diffusion plate to fix the light diffusion plate.

In a further improvement, the center of the LED array and the center line of the objective lens are positioned on the same optical axis

In a further improvement, the annular light shielding plate is arranged obliquely relatively to form a light cavity with a wide upper part and a narrow lower part.

In a further improvement, a perforated plate is mounted below the glass, and an objective lens is mounted below the perforated plate.

The use method of the multimode ultrathin light source device of the phase contrast microscope comprises the following steps:

step one, when phase contrast imaging is required, lightening a middle annular array in a transparent light guide ring;

and step two, when bright field imaging is required, lighting all the LED arrays.

Compared with the prior art, the invention has the beneficial effects that:

(1) the structure is simple and ultrathin, and the light source device is only 2cm thick.

(2) The multimode switching realizes the conversion between the phase contrast imaging and the bright field imaging by controlling the switch without adding any optical element in an imaging light path during the bright field imaging and the phase contrast imaging.

(3) When phase contrast imaging is carried out, the transparent light guide ring can ensure the illumination range, so that the illumination area is more accurate, and the measurement is more accurate.

Drawings

FIG. 1 is a schematic diagram of an embodiment of a phase contrast microscope multimode ultra-thin light source device according to the present invention;

FIG. 2 is a schematic structural diagram of an LED array, an LED base and a transparent light guide ring in the phase-contrast microscope multimode ultra-thin light source device of the present invention;

FIG. 3 is a circuit diagram of the present invention.

In the drawings, the components represented by the respective reference numerals are listed below:

the LED light source comprises a light source body, a light guide ring, a light cavity, an LED base, a 2-LED array, a 21-inner circular array, a 22-middle circular array, a 23-outer circular array, a 3-transparent light guide ring, a 4-light diffusion plate, 5-glass, a 6-porous plate, a 7-objective lens, an 8-light cavity, a 9-first switch and a 10-second switch.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1, the present invention provides a technical solution: the utility model provides a phase contrast microscope multi-mode ultra-thin light source device, includes LED base 1, LED array 2, transparent light guide ring 3, diffusion sheet 4 and glass 5, LED base 1 be circular, LED base 1 is the annular round hole that is distributing for LED array 2 just in time inlays wherein, transparent light guide ring 3's one end cover with well annular array 22 inside and outside both sides, on the ring, transparent light guide ring 3's the other end closely pastes with diffusion sheet 4, diffusion sheet 4 links to each other with glass 5.

The circle center of the LED array 2 and the central line of the objective lens 7 are positioned on the same optical axis.

The first embodiment is as follows: phase contrast imaging

Firstly, a phase contrast imaging mode is selected, then the middle ring array 22 of the LED array 2 of the circle where the transparent light guide ring 3 is located is lightened, the transparent light guide ring 2 can effectively prevent light emitted by the middle ring array 22 of the LED array 2 from diffusing outwards, light is more uniform after passing through the light diffusion plate 4, and the light irradiates on an object in the porous plate 6 after passing through the glass 5 and forms phase contrast imaging after being amplified by the objective lens 7.

Second embodiment bright field imaging

Firstly, a bright field imaging mode is selected, then the whole LED array 2 is lightened, light emitted by the LED array 2 is more uniform after passing through the light diffusion plate 4, and the light is irradiated on an object in the porous plate 6 after passing through the glass 5 and is amplified by the objective lens 7 to form bright field imaging.

The circuit schematic diagram of the present invention is that the LED lamps on the inner circular array 21 and the outer circular array 23 are arranged in parallel with the LED lamps on the middle circular array 22, and the LED lamps of the inner circular array 21 and the outer circular array 23 are electrically connected with the first switch 9, and the LED lamps on the middle circular array 22 are electrically connected with the second switch 10. In addition, all the LED lamps can be controlled independently to form illumination at different angles according to requirements.

In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims

1. A multi-mode ultrathin light source device of a phase contrast microscope comprises an LED base (1), wherein an LED array (2) is arranged on the LED base (1); the LED array (2) comprises an inner circular array (21), a middle annular array (22) is surrounded outside the inner circular array (21), and an outer annular array (23) is surrounded outside the middle annular array (22); annular light shading plates are fixed on the inner side and the outer side of the middle annular array (22); a light diffusion plate (4) is arranged below the light diffusion plate, and glass (5) is arranged below the light diffusion plate (4).

2. The phase-contrast microscope multimode ultrathin light source device as claimed in claim 1, characterized in that the annular light shielding plate is a transparent light guide ring (3), and black paint is sprayed on the inner wall and the outer wall of the transparent light guide ring (3).

3. The phase contrast microscope multimode ultra-thin light source device according to claim 2, characterized in that the LED array (2) is composed of a series of concentric LED lamps; the inner circular array (21), the middle annular array (22) and the outer circular array (23) are all concentrically arranged.

4. The phase contrast microscope multimode ultra-thin light source device according to claim 3, characterized in that the middle ring array (22) is a third ring array of LED arrays (2); the transparent light guide rings (3) are embedded on the inner side and the outer side of the third ring array.

5. The multimode ultra-thin light source device of the phase contrast microscope according to claim 1, wherein the light diffusing plate (4) is closely connected with the lower end of the transparent light guiding ring (3) to prevent light from diffusing outwards.

6. The multimode ultra-thin light source device of phase contrast microscope according to claim 1, wherein the glass (5) is closely attached to the light-diffusing plate (4) to fix the light-diffusing plate (4).

7. The phase-contrast microscope multimode ultra-thin light source device of claim 1, characterized in that: the circle center of the LED array (2) and the central line of the objective lens (7) are positioned on the same optical axis.

8. The phase-contrast microscope multimode ultra-thin light source device of claim 1, characterized in that: the annular light shielding plates are arranged oppositely in an inclined mode to form a light cavity (8) which is wide at the top and narrow at the bottom.

9. The phase-contrast microscope multimode ultra-thin light source device of claim 1, characterized in that: a porous plate (6) is installed below the glass (5), and an objective lens (7) is installed below the porous plate (6).

10. The use method of the phase contrast microscope multimode ultrathin light source device is characterized in that: the method comprises the following steps:

step one, when phase contrast imaging is required, lightening a middle annular array (22) in a transparent light guide ring (3);

and step two, when bright field imaging is required, all the LED arrays (2) are lightened.