CN111006927A - Method for reducing interference of fat residue on fish body micro-plastic detection - Google Patents
Method for reducing interference of fat residue on fish body micro-plastic detection Download PDFInfo
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- CN111006927A CN111006927A CN201911377908.3A CN201911377908A CN111006927A CN 111006927 A CN111006927 A CN 111006927A CN 201911377908 A CN201911377908 A CN 201911377908A CN 111006927 A CN111006927 A CN 111006927A
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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Abstract
The invention provides a method for reducing interference of fat residue on fish body micro-plastic detection. The method comprises treatment with a surfactant and/or ultrasound. Specifically, the method comprises the following steps: the fish body micro plastic is selected from fish body digestive gland; the surfactant is preferably sodium dodecylbenzene sulfonate. The concentration of the surfactant is 0.1-3 g/ml; further 0.5 to 2g/ml (1 g/ml); the ultrasonic treatment is 10-20 KHz (preferably 15KHz), and the ultrasonic treatment is carried out for 20-60 minutes (preferably 30 minutes). The invention reduces the influence of the grease on the detection of the micro-plastics of the digestive gland of the fish body by adopting a means of combining the surfactant and the ultrasound. Greatly improving the filtering speed and improving the detection environment. Therefore, it has good practical application value.
Description
Technical Field
The invention belongs to the technical field of detection and analysis, and particularly relates to a method for reducing interference of fat residues on fish body micro-plastic detection.
Background
The information in this background section is only for enhancement of understanding of the general background of the invention and is not necessarily to be construed as an admission or any form of suggestion that this information forms the prior art that is already known to a person of ordinary skill in the art.
Due to human life and production activities, a large number of plastic products enter the environment, wherein the plastic products with the size less than 5mm are defined as micro plastic, the sources of the micro plastic comprise daily chemical products, such as toothpaste, cleansing and the like, addition and use, fiber shedding of textile products, tire abrasion and the like, and a large part of the micro plastic products are generated by aging and fragmentation of the plastic products under the physical and chemical effects of sunlight, sea waves and the like. The ocean is a sink of micro-plastics, and a large amount of micro-plastics eventually sink into the ocean. The tiny size of the micro plastic is easy to be swallowed by marine organisms and is transmitted through a food chain, so that the micro plastic becomes an environmental hotspot problem which is concerned by the academic community in recent years. However, to clarify the environmental problem of the micro-plastics, it is an important step to accurately obtain the content and distribution characteristics of the micro-plastics in the organism. The current research mainly focuses on the detection of the micro-plastics in digestive glands of fish bodies, and as the field is still in the research starting stage, a plurality of technical problems still exist and cannot be solved.
The core technical goal of the fish body micro-plastic detection is to remove the interference of contents of digestive glands of fish bodies by a digestion means and obtain the micro-plastic in the digestive glands by filtering and separating. The technical difficulty is that on one hand, digestive gland digestion does not completely interfere detection and seriously influences the filtering speed, and on the other hand, digestion reagents influence the physicochemical property of plastics to cause false negative. Particularly, the experiment efficiency and sample separation detection are seriously influenced by membrane blockage caused by high fat content of part of fish species, and a learner adopts a high-temperature filtration method to improve the filtration rate, but the method has obvious influence on part of plastic polymers with poor thermal stability due to overhigh temperature, and can cause the phenomena of plastic dissolution, softening and the like.
Disclosure of Invention
Based on the defects of the prior art, the invention provides a method for reducing the interference of fat residues on the detection of the fish body micro-plastics. Greatly improving the filtering speed and improving the detection environment. Therefore, it has good practical application value.
In one aspect of the invention, a method is provided for reducing interference of fat residue on fish microplastic detection, the method comprising treatment with a surfactant and/or ultrasound.
Specifically, the method comprises the following steps:
the fish body micro plastic is selected from fish body digestive gland;
the surfactant includes but is not limited to anionic surfactant, cationic surfactant, zwitterionic surfactant and nonionic surfactant, and further, the surfactant is stearic acid, sodium dodecyl benzene sulfonate, lecithin, polysorbate (tween); sodium dodecylbenzenesulfonate is preferred.
The concentration of the surfactant is 0.1-3 g/ml; further 0.5 to 2g/ml (1 g/ml);
the ultrasonic treatment is 10-20 KHz (preferably 15KHz), and the ultrasonic treatment is carried out for 20-60 minutes (preferably 30 minutes).
Furthermore, the ultrasonic treatment and the treatment of adding the surfactant are carried out simultaneously, so that the co-treatment time is 20-60 minutes (preferably 30 minutes) in the invention.
Further, the method comprises: when the micro-plastic of the digestive tract of the fish body is detected, after the digestive gland of the fish body is digested, the digestive gland of the fish body is treated by adopting a surfactant and/or ultrasonic waves, so that the interference on the detection of the micro-plastic of the fish body is reduced.
The surfactant includes but is not limited to anionic surfactant, cationic surfactant, zwitterionic surfactant and nonionic surfactant, and further, the surfactant is stearic acid, sodium dodecyl benzene sulfonate, lecithin, polysorbate (tween); sodium dodecylbenzenesulfonate is preferred.
The concentration of the surfactant is 0.1-3 g/ml; further 0.5 to 2g/ml (1 g/ml);
the ultrasonic treatment is 10-20 KHz (preferably 15KHz), and the ultrasonic treatment is carried out for 20-60 minutes (preferably 30 minutes).
Furthermore, the ultrasonic treatment and the treatment of adding the surfactant are carried out simultaneously, so that the co-treatment time is 20-60 minutes (preferably 30 minutes) in the invention.
In a second aspect of the invention, there is provided the use of a co-treatment of a surfactant and ultrasound to reduce interference of fat residues with detection of fish microplastics.
The fish body micro plastic is selected from fish body digestive gland;
the surfactant includes but is not limited to anionic surfactant, cationic surfactant, zwitterionic surfactant and nonionic surfactant, and further, the surfactant is stearic acid, sodium dodecyl benzene sulfonate, lecithin, polysorbate (tween); sodium dodecylbenzenesulfonate is preferred.
The concentration of the surfactant is 0.1-3 g/ml; further 0.5 to 2g/ml (1 g/ml);
the ultrasonic treatment is 10-20 KHz (preferably 15KHz), and the ultrasonic treatment is carried out for 20-60 minutes (preferably 30 minutes).
The ultrasonic treatment and the treatment with the surfactant are carried out simultaneously, so that the co-treatment time is 20-60 minutes (preferably 30 minutes) in the invention.
The invention has the beneficial technical effects that:
the invention reports that when the micro-plastic detection is carried out on the digestive tract of a fish body for the first time, in order to reduce the grease interference condition, the ultrasonic treatment and the surfactant addition co-treatment are adopted. Therefore, the interference of grease is reduced, and the influence on the micro plastic is avoided, so that the micro plastic has good practical application value.
Detailed Description
It is to be understood that the following detailed description is exemplary and is intended to provide further explanation of the invention as claimed. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments in accordance with the present application. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.
As mentioned above, the interference of the contents of digestive gland of fish body is removed, and the micro plastic in digestive gland is obtained by filtration and separation. The technical difficulty is that on one hand, digestive gland digestion does not completely interfere detection and seriously influences the filtering speed, and on the other hand, digestion reagents influence the physicochemical property of plastics to cause false negative. Particularly, the experiment efficiency and sample separation detection are seriously influenced by membrane blockage caused by high fat content of part of fish species, and a learner adopts a high-temperature filtration method to improve the filtration rate, but the method has obvious influence on part of plastic polymers with poor thermal stability due to overhigh temperature, and can cause the phenomena of plastic dissolution, softening and the like.
In view of the above, in one exemplary embodiment of the present invention, a method for reducing interference of fat residue on detection of fish microplastics is provided, the method comprising treatment with a surfactant and/or ultrasound.
In yet another embodiment of the present invention, the method comprises:
the fish body micro plastic is selected from fish body digestive gland;
the surfactant includes but is not limited to anionic surfactant, cationic surfactant, zwitterionic surfactant and nonionic surfactant, and further, the surfactant is stearic acid, sodium dodecyl benzene sulfonate, lecithin, polysorbate (tween); sodium dodecylbenzenesulfonate is preferred.
The concentration of the surfactant is 0.1-3 g/ml; further 0.5 to 2g/ml (1 g/ml);
the ultrasonic treatment is 10-20 KHz (preferably 15KHz), and the ultrasonic treatment is carried out for 20-60 minutes (preferably 30 minutes).
In another embodiment of the present invention, the ultrasonic treatment is performed simultaneously with the surfactant addition treatment, and thus the co-treatment time in the present invention is 20 to 60 minutes (preferably 30 minutes).
In yet another embodiment of the present invention, the method comprises: when the micro-plastic of the digestive tract of the fish body is detected, after the digestive gland of the fish body is digested, the digestive gland of the fish body is treated by adopting a surfactant and/or ultrasonic waves, so that the interference on the detection of the micro-plastic of the fish body is reduced.
The surfactant includes but is not limited to anionic surfactant, cationic surfactant, zwitterionic surfactant and nonionic surfactant, and further, the surfactant is stearic acid, sodium dodecyl benzene sulfonate, lecithin, polysorbate (tween); sodium dodecylbenzenesulfonate is preferred.
The concentration of the surfactant is 0.1-3 g/ml; further 0.5 to 2g/ml (1 g/ml);
the ultrasonic treatment is 10-20 KHz (preferably 15KHz), and the ultrasonic treatment is carried out for 20-60 minutes (preferably 30 minutes).
In another embodiment of the present invention, the ultrasonic treatment is performed simultaneously with the surfactant addition treatment, and thus the co-treatment time in the present invention is 20 to 60 minutes (preferably 30 minutes).
In yet another embodiment of the present invention, there is provided the use of co-treatment with a surfactant and ultrasound to reduce interference of fat residues with detection of fish microplastics.
The fish body micro plastic is selected from fish body digestive gland;
the surfactant includes but is not limited to anionic surfactant, cationic surfactant, zwitterionic surfactant and nonionic surfactant, and further, the surfactant is stearic acid, sodium dodecyl benzene sulfonate, lecithin, polysorbate (tween); sodium dodecylbenzenesulfonate is preferred.
The concentration of the surfactant is 0.1-3 g/ml; further 0.5 to 2g/ml (1 g/ml);
the ultrasonic treatment is 10-20 KHz (preferably 15KHz), and the ultrasonic treatment is carried out for 20-60 minutes (preferably 30 minutes).
The ultrasonic treatment and the treatment with the surfactant are carried out simultaneously, so that the co-treatment time is 20-60 minutes (preferably 30 minutes) in the invention.
The invention is further illustrated by the following examples, which are not to be construed as limiting the invention thereto. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention.
Example 1
When the micro-plastic of the digestive tract of the fish body is detected, after the digestive gland of the fish body is digested, the digestive gland of the fish body is treated by adopting a surfactant and/or ultrasonic waves, so that the interference on the detection of the micro-plastic of the fish body is reduced.
The surfactant is sodium dodecyl benzene sulfonate; the concentration of the surfactant is 0.5 g/ml;
the ultrasonic treatment frequency is 15KHz, and the ultrasonic treatment time is 30 minutes.
Example 2
When the micro-plastic of the digestive tract of the fish body is detected, after the digestive gland of the fish body is digested, the digestive gland of the fish body is treated by adopting a surfactant and/or ultrasonic waves, so that the interference on the detection of the micro-plastic of the fish body is reduced.
The surfactant is sodium dodecyl sulfate; the concentration of the surfactant is 2 g/ml;
the ultrasonic treatment frequency is 15KHz, and the ultrasonic treatment time is 30 minutes.
Example 3
When the micro-plastic of the digestive tract of the fish body is detected, after the digestive gland of the fish body is digested, the digestive gland of the fish body is treated by adopting a surfactant and/or ultrasonic waves, so that the interference on the detection of the micro-plastic of the fish body is reduced.
The surfactant is sodium dodecyl benzene sulfonate; the concentration of the surfactant is 1 g/ml;
the ultrasonic treatment frequency is 15KHz, and the ultrasonic treatment time is 30 minutes.
It should be noted that the above-mentioned embodiments are only preferred embodiments of the present invention, and the present invention is not limited thereto, and although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and equivalents can be made in the technical solutions described in the foregoing embodiments, or equivalents thereof. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention. Although the present invention has been described with reference to the specific embodiments, it should be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention.
Claims (10)
1. A method of reducing interference of fat residues on detection of fish microplastics, comprising treatment with a surfactant and/or ultrasound.
2. The method of claim 1, wherein the method comprises:
the fish body micro plastic is selected from fish body digestive gland.
3. The method of claim 1, wherein the method comprises:
the surfactant includes but is not limited to anionic surfactant, cationic surfactant, zwitterionic surfactant and nonionic surfactant, and further, the surfactant is stearic acid, sodium dodecyl benzene sulfonate, lecithin, polysorbate (tween); sodium dodecylbenzenesulfonate is preferred.
4. The method of claim 1, wherein the surfactant concentration is 0.1 to 3 g/ml; further 0.5 to 2g/ml (1 g/ml).
5. The method of claim 1, wherein the sonication is at 10 to 20KHz (preferably 15KHz) for 20 to 60 minutes (preferably 30 minutes).
6. The method of claim 1, wherein the method comprises: when the micro-plastics of the digestive tract of the fish body are detected, after the digestive gland of the fish body is digested, a surfactant and/or ultrasound is adopted for processing.
7. The method of claim 6, wherein the method comprises: the surfactant includes but is not limited to anionic surfactant, cationic surfactant, zwitterionic surfactant and nonionic surfactant, and further, the surfactant is stearic acid, sodium dodecyl benzene sulfonate, lecithin, polysorbate (tween); preferably sodium dodecyl benzene sulfonate;
the concentration of the surfactant is 0.1-3 g/ml; further 0.5 to 2g/ml (1 g/ml);
the ultrasonic treatment is 10-20 KHz (preferably 15KHz), and the ultrasonic treatment is carried out for 20-60 minutes (preferably 30 minutes);
preferably, the ultrasonic treatment and the treatment with the surfactant are carried out simultaneously, and the co-treatment time is 20-60 minutes (preferably 30 minutes).
8. The application of the co-treatment of the surfactant and the ultrasound in reducing the interference of fat residue on the detection of the fish body micro-plastics.
9. The use of claim 8, wherein the fish body micropolastic is selected from the group consisting of fish body digestive glands.
10. The use of claim 8, wherein the surfactant includes but is not limited to anionic, cationic, zwitterionic and nonionic surfactants, and further wherein the surfactant is stearic acid, sodium dodecylbenzenesulfonate, lecithin, polysorbate (tween); preferably sodium dodecyl benzene sulfonate;
the concentration of the surfactant is 0.1-3 g/ml; further 0.5 to 2g/ml (1 g/ml);
the ultrasonic treatment is 10-20 KHz (preferably 15KHz), and the ultrasonic treatment is carried out for 20-60 minutes (preferably 30 minutes);
the co-treatment time is 20-60 minutes (preferably 30 minutes) when the ultrasonic treatment and the surfactant addition treatment are carried out simultaneously.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911377908.3A CN111006927A (en) | 2019-12-27 | 2019-12-27 | Method for reducing interference of fat residue on fish body micro-plastic detection |
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| CN201911377908.3A CN111006927A (en) | 2019-12-27 | 2019-12-27 | Method for reducing interference of fat residue on fish body micro-plastic detection |
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Cited By (1)
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| CN111504741A (en) * | 2020-04-22 | 2020-08-07 | 山东师范大学 | Method for detecting micro-plastics in fish body |
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Application publication date: 20200414 |