CN110999788A - Method for rapidly propagating wintersweet plants - Google Patents
Method for rapidly propagating wintersweet plants Download PDFInfo
- Publication number
- CN110999788A CN110999788A CN201911268882.9A CN201911268882A CN110999788A CN 110999788 A CN110999788 A CN 110999788A CN 201911268882 A CN201911268882 A CN 201911268882A CN 110999788 A CN110999788 A CN 110999788A
- Authority
- CN
- China
- Prior art keywords
- wintersweet
- stem
- seedling
- segments
- rapidly propagating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a method for rapidly propagating wintersweet plants, which relates to the technical field of rapid propagation of plants. The invention has the beneficial effects that: the operation is simple, and the cost is low; the raw materials are few, and the materials are rich; the propagation speed is high, the propagation coefficient is high, the propagation is not limited by seasons and the like, and the method has important significance for propagation of wintersweet seedlings and large-scale propagation of later-stage excellent strains.
Description
Technical Field
The invention relates to the technical field of plant rapid propagation, in particular to a method for rapidly propagating wintersweet plants.
Background
Chimonanthus praecox (Linn.) Link is also called Chimonanthus praecox, when flowers bloom in cold winter, the flowers are yellow like wax, the fragrance overflows, the shapes are beautiful, the flowering period is long, the Chimonanthus praecox is special precious flowers and trees in China, and the Chimonanthus praecox is also a famous garden ornamental tree species and cut flower tree species in the world. Because wintersweet is pleasant in fragrance, the essence components extracted by the wintersweet are richer than those of rose oil and jasmine flower fragrance, and the price of the wintersweet aromatic oil in the international market is greatly higher than that of the rose oil and the jasmine aromatic oil. The roots, stems, leaves, flower buds and fruits of wintersweet can all be used as human medicines, and have the effects of relieving summer-heat, promoting the production of body fluid, regulating qi and relieving cough. In addition, the wintersweet has developed root system and strong adaptability, can resist various harmful gases, is a multifunctional tree species integrating ornamental, medicinal and environmental protection values, and has wide application prospect.
At present, in production, the breeding of the wintersweet mainly adopts traditional methods such as sowing, grafting and splitting, but the conventional breeding means has the problems of complicated breeding process, low breeding coefficient, season limitation and the like, and the demand of the market on the wintersweet seedlings and the rapid development of the wintersweet industry can not be met. At present, researches on propagation of wintersweet by using a cuttage technology have been reported, but the problems of limited propagation materials, difficult rooting, low rooting efficiency, season limitation on propagation and the like exist generally. In addition, the in vitro regeneration system of the wintersweet plant is established by utilizing the plant tissue culture technology in the earlier stage, but the problems of complicated propagation process, longer period, higher cost and the like exist in the propagation process. Aiming at various problems in the wintersweet breeding process, a wintersweet breeding method which is simple in operation, high in breeding speed, short in breeding period, few in raw material drawing and low in cost needs to be developed urgently to meet the requirement of large-scale planting on wintersweet seedlings.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a method for in vitro regeneration of wintersweet plants, which has high propagation speed and high propagation efficiency.
The invention adopts the following technical scheme to solve the technical problems:
the invention provides a method for rapidly propagating wintersweet plants, which comprises the following steps: the stem section of the wintersweet tissue culture regenerated seedling or the stem section of the wintersweet seed seedling is used as an explant source, and after the wintersweet tissue culture regenerated seedling or the wintersweet seed seedling is cut into sections, wintersweet plants are obtained through surface disinfection, adventitious root induction treatment, field planting and seedling management.
Preferably, the stem section is obtained from a wintersweet tissue culture regenerated seedling with the seedling age of 3-24 months or a 1-2-year wintersweet seed seedling.
Preferably, the cutting into the cut pieces is a cut piece of a stem piece with 2 axillary buds and 2 leaves, which is cut into a length of 1.0-3.0 cm.
Preferably, the surface disinfection is to wash the stem segments with running water, disinfect the stem segments with an aqueous solution added with 0.01-0.05% of carbendazim and 0.01-0.2% of potassium permanganate for 1-3min, and air-dry the residual solution on the stem segments.
Preferably, the adventitious root induction treatment is to immerse the morphological lower end of the stem segment in a rooting promoting liquid added with 5-10mg/L of indoleacetic acid, 20-100mg/L of melatonin, 100-300mg/L of indolebutyric acid, 0.01-0.5mg/L of VB12 and 0.3-0.6 per mill of Tween-20 to carry out the adventitious root induction treatment.
Preferably, the time for the adventitious root induction treatment is 20 to 90 s.
Preferably, the permanent planting is to plant the morphological lower end of the wintersweet stem section after the adventitious root induction treatment in a nutrient medium plate filled with a nutrient medium.
Preferably, the nutrient medium comprises nutrient soil and vermiculite, and the mass ratio of the nutrient soil to the vermiculite is 2: 1.
Preferably, the stem seedling management comprises the steps of spraying nutrient solution once on the stem after field planting, covering a cover, placing in a greenhouse with the temperature of 20-23 ℃ and the relative humidity of 85-90% for seedling culture, watering for 1 time every 5-7 days, and removing the plastic cover after 1 month.
Preferably, the nutrient solution comprises 50% of Hoagland nutrient solution prepared by double distilled water.
The invention has the beneficial effects that:
(1) the operation is simple, the material is obtained less, the material is obtained conveniently, and the material is rich;
(2) the induction rate of the adventitious roots is as high as 98.3%, and each explant averagely generates 2-4 adventitious roots;
(3) the regeneration speed is high, the seedlings can be grown in 5 weeks, the propagation coefficient is high, the obtained regenerated plants can be further expanded and propagated, and the technical support is provided for the rapid propagation of wintersweet plants and the large-scale production of later-stage excellent plants.
Drawings
FIG. 1 is a cut view of a wintersweet stem segment in example 2 of the present invention;
FIG. 2 is a diagram showing an initial rooting stage of a wintersweet stem segment in example 2 of the present invention;
FIG. 3 is a diagram showing the seedling formation of a wintersweet stem section in example 2 of the present invention;
FIG. 4 is a rooting chart corresponding to the seedling formation of the wintersweet stem segment in example 2 of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Test materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
The specific techniques or conditions not specified in the examples can be performed according to the techniques or conditions described in the literature in the field or according to the product specification.
Example 1
A method for rapidly propagating Chimonanthus praecox plants comprises the following steps:
(1) cutting stem segments into 1.0cm long cut segments with 2 axillary buds and 2 leaves for later use by taking wintersweet strain tissue culture seedlings with the seedling age of 3 months as explant sources; wherein the tissue culture seedling is obtained by a method for high-efficiency in-vitro regeneration of a Chimonanthus nitens plant in patent CN 201710305050.4;
(2) washing the stem sections in the step (1) with running water, disinfecting for 1min by using water added with 0.01% of carbendazim and 0.01% of potassium permanganate, and airing residual solution on the surfaces of the stem sections for later use;
(3) performing adventitious root induction treatment on the stem segments sterilized in the step (2), namely soaking the morphological lower ends of the stem segments in rooting promoting liquid added with 5mg/L indoleacetic acid, 20mg/L melatonin, 100mg/L indolebutyric acid, 0.01mg/L VB12 and 0.3 per thousand Tween-20 for adventitious root induction treatment for 90 s;
(4) planting the morphological lower end of the stem section of the wintersweet tissue culture seedling subjected to adventitious root induction treatment in the step (3) in a nutrient medium (nutrient soil: vermiculite: 2:1) disc, wherein the length of the inserted stem section is 0.5cm on the basis of no overlapping;
(5) spraying a nutrient solution once on the stem segments subjected to field planting in the step (4), wherein the nutrient solution comprises 50% of Hoagland nutrient solution, covering a plastic cover, placing in a greenhouse with the temperature of 20-23 ℃ and the relative humidity of 85-90% for seedling culture, and watering for 1 time every 5-7 days; after 3 weeks of culture, adventitious roots with the length of 0.2-0.5cm are formed at the base of the stem section; after further culturing for 2 weeks, adventitious roots with a length of about 2-3cm were produced at the base of the stem segment, and a complete regenerated plant was obtained.
The induction rate of the adventitious roots in this example is as high as 74.2%, and on average 1-3 adventitious roots are generated per explant.
Example 2
A method for rapidly propagating Chimonanthus praecox plants comprises the following steps:
(1) cutting stem segments into 2.0cm long sections with 2 axillary buds and 2 leaves for later use by taking wintersweet strain tissue culture seedlings with the seedling age of 12 months as explant sources; wherein the tissue culture seedling is obtained by a method for high-efficiency in-vitro regeneration of a Chimonanthus nitens plant in patent CN 201710305050.4;
(2) washing the stem segments in the step (1) with running water, disinfecting the stem segments with water added with 0.02% of carbendazim and 0.05% of potassium permanganate for 2min, airing residual solution on the surfaces of the stem segments for later use, and cutting off the stem segments as shown in a figure 1;
(3) performing adventitious root induction treatment on the stem segments sterilized in the step (2), namely soaking the morphological lower ends of the stem segments in rooting promoting liquid added with 7.5mg/L of indoleacetic acid, 60mg/L of melatonin, 200mg/L of indolebutyric acid, 0.1mg/L of VB12 and 0.4 per thousand of Tween-20 for adventitious root induction treatment for 60 s;
(4) planting the morphological lower end of the stem section of the wintersweet tissue culture seedling subjected to adventitious root induction treatment in the step (3) in a nutrient medium (nutrient soil: vermiculite: 2:1) disc, wherein the length of the inserted stem section is 0.75cm on the basis of no overlapping;
(5) spraying a nutrient solution once on the stem segments planted in the step (4), wherein the nutrient solution is a Hoagland nutrient solution with the nutrient content of 50%, covering a plastic cover, placing the stem segments in a greenhouse with the temperature of 20-23 ℃ and the relative humidity of 85-90% for seedling culture, and watering 1 time every 5-7 days; as shown in FIG. 2, after 3 weeks of cultivation, adventitious roots with a length of about 0.5-1.0cm were formed at the base of the stem segment; after further culturing for 2 weeks, adventitious roots with the length of about 3-5cm are generated at the base of the stem segment, and a complete regeneration plant is obtained, fig. 3 is a seedling graph of the wintersweet stem segment in the embodiment, and fig. 4 is a rooting graph corresponding to seedling of the wintersweet stem segment.
In this example, the induction rate of adventitious roots is as high as 98.3%, and 2-4 adventitious roots are generated per explant on average.
Example 3
A method for rapidly propagating Chimonanthus praecox plants comprises the following steps:
(1) cutting stem segment into 3.0cm long cut segment with 2 axillary buds and 2 leaves for use with 1-2 years old wintersweet seed seedling as explant source;
(2) washing the stem sections in the step (1) with running water, disinfecting for 3min by using 0.05% of carbendazim and 0.2% of potassium permanganate, and airing residual solution on the surfaces of the stem sections for later use;
(3) performing adventitious root induction treatment on the stem segments sterilized in the step (2), namely soaking the morphological lower ends of the stem segments in rooting promoting liquid added with 10mg/L indoleacetic acid, 100mg/L melatonin, 300mg/L indolebutyric acid, 0.5mg/L VB12 and 0.6 per thousand Tween-20 for adventitious root induction treatment for 20 s;
(4) planting the morphological lower end of the wintersweet stem section subjected to adventitious root induction treatment in the step (3) in a nutrient medium (nutrient soil: vermiculite: 2:1) disc, wherein the length of the inserted stem section is 1.0cm on the basis of no overlapping;
(5) spraying a nutrient solution once on the stem segments planted in the step (4), wherein the nutrient solution is a Hoagland nutrient solution with the nutrient content of 50%, covering a plastic cover, placing the stem segments in a greenhouse with the temperature of 20-23 ℃ and the relative humidity of 85-90% for seedling culture, and watering 1 time every 5-7 days; after 3 weeks of culture, adventitious roots with a length of about 1.0cm are formed at the base of the stem section; after further culturing for 2 weeks, adventitious roots with a length of about 2-4cm were produced at the base of the stem segment, and a complete regenerated plant was obtained.
In this example, the induction rate of adventitious roots is as high as 92.5%, and 2-4 adventitious roots are generated per explant on average.
Example 4
A method for rapidly propagating Chimonanthus praecox plants comprises the following steps:
(1) taking the wintersweet regenerated plant obtained by the method as an explant source, cutting a stem segment into a cut segment with the length of 1.5cm and 2 axillary buds and 2 leaves for later use;
(2) washing the stem sections in the step (1) with running water, disinfecting for 1min by using water added with 0.02% of carbendazim and 0.1% of potassium permanganate, and airing residual solution on the surfaces of the stem sections for later use;
(3) performing adventitious root induction treatment on the stem segments sterilized in the step (2), namely soaking the morphological lower ends of the stem segments in rooting promoting liquid added with 7.5mg/L of indoleacetic acid, 50mg/L of melatonin, 150mg/L of indolebutyric acid, 0.2mg/L of VB12 and 0.3 per thousand of Tween-20 for adventitious root induction treatment, wherein the treatment time is 40 s;
(4) planting the morphological lower end of the stem section of the wintersweet tissue culture seedling subjected to adventitious root induction treatment in the step (3) in a nutrient medium (nutrient soil: vermiculite: 2:1) disc, wherein the length of the inserted stem section is 0.5cm on the basis of no overlapping;
(5) spraying a nutrient solution once on the stem segments planted in the step (4), wherein the nutrient solution is a Hoagland nutrient solution with the nutrient content of 50%, covering a plastic cover, placing the stem segments in a greenhouse with the temperature of 20-23 ℃ and the relative humidity of 85-90% for seedling culture, and watering 1 time every 5-7 days; after 2 weeks of culture, adventitious root primordium is formed at the base of the stem section; after further culturing for 3 weeks, adventitious roots with a length of about 3-5cm were produced at the base of the stem segment, and a complete regenerated plant was obtained.
In this example, the induction rate of adventitious roots is as high as 95.8%, and 2-4 adventitious roots are generated per explant on average.
EXAMPLE 5
Determining the influence of the species, the concentration and the treatment time of the rooting inducer in the rooting promoting liquid on the formation of the adventitious roots of the wintersweet stem segments:
firstly, only changing the species and the concentration of a rooting inducer in a rooting promoting liquid, taking a wintersweet strain tissue culture seedling with the seedling age of 12 months as an explant source, cutting a stem section into 2.0cm cut sections with 2 axillary buds and 2 leaves, and treating the base part of the stem section in the rooting promoting liquid added with the rooting inducer with different species and concentrations for 60s after surface disinfection (the steps are the same as the step 2). And 5 weeks after the cultivation, counting the adventitious root induction rate of the wintersweet stem segment.
The measurement results are shown in table 1, and the results in table 1 show that the variety and concentration of the adventitious root inducing solution play a key role in inducing adventitious roots of wintersweet stem segments, and certain synergistic promotion effects exist among the used rooting inducers. Wherein for the tissue culture seedling stem segment of a wintersweet strain with the seedling age of 12 months, the morphological lower end of the stem segment is immersed in rooting promoting liquid added with 7.5mg/L indoleacetic acid, 60mg/L melatonin, 200mg/L indolebutyric acid, 0.1mg/L VB12 and 0.4 thousandth Tween-20 to carry out adventitious root induction, and the induction effect is optimal.
Table 1 shows the influence of the variety and concentration of the adventitious root inducing solution on the induction of adventitious roots in wintersweet stem segments
Each treatment was repeated 3 times, 40 explants per treatment, for a total of 120 explants.
Secondly, only the treatment time of the wintersweet stem segments in the rooting promoting liquid is changed, the wintersweet strain tissue culture seedlings with the seedling age of 12 months are taken as explant sources, the stem segments are cut into 2.0cm cut segments with 2 axillary buds and 2 leaves, and the cut segments are subjected to surface disinfection and then treated in the rooting promoting liquid for different time periods (10s, 20s,30s,40s,60s,90s and 120s) related to the embodiment 2. The rest of the procedure was the same as in example 2. And 5 weeks after the cultivation, counting the adventitious root induction rate of the wintersweet stem segment.
The measurement results are shown in table 2, and the results in table 2 show that the treatment time of the adventitious root inducing solution plays an important role in inducing the adventitious root of the wintersweet stem segment. The induction rate of the adventitious roots gradually increases with the increase of the treatment time within a certain treatment time, and the induction rate of the adventitious roots is as high as 98.3% when the adventitious roots are treated for 60s, but the induction rate of the adventitious roots tends to decrease with the increase of the treatment time.
Table 2 shows the influence of the treatment time of the adventitious root inducing solution on the induction of adventitious roots in wintersweet stems
Each treatment was repeated 3 times, 40 explants per treatment, for a total of 120 explants.
Example 6
Determining the influence of the length of the wintersweet plant stem on the formation of adventitious roots
Only the length of the stem segment is changed, the tissue culture seedling of the wintersweet strain with the seedling age of 24 months is taken as the explant source, and the stem segment is cut into cut segments with 2 axillary buds and 2 leaves, wherein the cut segments are 0.3,0.5,1.0,2.0,3.0,5.0 and 8.0cm for standby. The rest of the procedure was the same as in example 2. After culturing for 5 weeks, counting the induction rate of adventitious roots and the number of adventitious roots of the wintersweet stem segment.
The results of the measurement are shown in Table 3, and the results in Table 3 indicate that the length of the explant of the stem segment used has an important influence on the induction of adventitious roots, and that the length of the adventitious roots is too short to be beneficial to the induction of the adventitious roots.
When the length of the used wintersweet stem segment is 0.3cm and 0.5cm, the inductivity of the adventitious root is 14.2 percent and 43.3 percent respectively, and the average number of the adventitious root is 1.21 and 1.73 respectively. The stem segments are too short to be planted in the nutrient medium, so that the absorption of water and nutrients by the stem segments in the adventitious root induction process is not facilitated. Further research shows that the induction rate of the adventitious roots is gradually increased within a certain range along with the increase of the stem length, the induction effect is optimal when the stem length reaches 3.0cm, the induction rate of the adventitious roots is 98.3%, and the number of the adventitious roots is 3.71. The induction rate of the adventitious roots and the number of the adventitious roots tend to gradually decrease with the further increase of the length of the stem segment, the nutrients consumed by the stem segment in the induction process of the adventitious roots gradually increase with the increase of the length of the stem segment, and the nutrients absorbed by the base of the stem segment are insufficient to meet the requirements of the formation and growth of the adventitious roots. Therefore, the propagation of the wintersweet is carried out by selecting and using the 1.0-3.0cm long wintersweet stem section as the material, so that the experimental material can be saved and the propagation coefficient of the wintersweet plant can be enlarged.
Table 3 shows the effect of wintersweet stem length on adventitious root induction
Each treatment was repeated 3 times, 40 explants per treatment, for a total of 120 explants.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (9)
1. A method for rapidly propagating Chimonanthus praecox plants is characterized in that: the method comprises the following steps: the stem section of the wintersweet tissue culture regenerated seedling or the stem section of the seed seedling is used as an explant source, and after the stem section or the seed seedling is cut into sections, wintersweet plants are obtained through surface disinfection, adventitious root induction treatment, field planting and seedling management.
2. A method of rapidly propagating wintersweet plants according to claim 1, characterized in that: the stem section is obtained from tissue culture regeneration seedlings of wintersweet with the seedling age of 3-24 months or seedlings of 1-2 year-old wintersweet seeds.
3. A method of rapidly propagating wintersweet plants according to claim 1, characterized in that: the cutting into segments is cutting stem segments into segments with 2 axillary buds and 2 leaves, the length of each stem segment is 1.0-3.0 cm.
4. A method of rapidly propagating wintersweet plants according to claim 1, characterized in that: the surface disinfection is to wash the stem segments with running water, disinfect the stem segments with an aqueous solution added with 0.01 to 0.05 percent of carbendazim and 0.01 to 0.2 percent of potassium permanganate for 1 to 3min, and air-dry the residual solution on the stem segments.
5. A method of rapidly propagating wintersweet plants according to claim 1, characterized in that: the adventitious root induction treatment is to immerse the morphological lower end of the stem segment in rooting promoting liquid added with 5-10mg/L of indoleacetic acid, 20-100mg/L of melatonin, 100-300mg/L of indolebutyric acid, 0.01-0.5mg/L of VB12 and 0.3-0.6 per thousand of Tween-20 to carry out adventitious root induction treatment.
6. A method of rapidly propagating Chimonanthus praecox plants as claimed in claim 5, wherein: the time of the adventitious root induction treatment is 20-90 s.
7. A method of rapidly propagating wintersweet plants according to claim 1, characterized in that: the permanent planting is that the morphological lower end of the wintersweet stem section after the adventitious root induction treatment is fixedly planted in a nutrient medium plate filled with nutrient medium.
8. A method of rapidly propagating wintersweet plants according to claim 7, characterized in that: the nutrient medium comprises nutrient soil and vermiculite, and the mass ratio of the nutrient soil to the vermiculite is 2: 1.
9. A method of rapidly propagating wintersweet plants according to claim 1, characterized in that: the stem seedling management comprises the steps of spraying 50% Hoagland nutrient solution prepared by double distilled water once on the planted stems, covering the stems with a cover, placing the stems in a greenhouse with the temperature of 20-23 ℃ and the relative humidity of 85-90% for seedling culture, watering 1 time every 5-7 days, and removing a plastic cover after 1 month.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911268882.9A CN110999788B (en) | 2019-12-11 | 2019-12-11 | Method for rapidly propagating wintersweet plants |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911268882.9A CN110999788B (en) | 2019-12-11 | 2019-12-11 | Method for rapidly propagating wintersweet plants |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110999788A true CN110999788A (en) | 2020-04-14 |
| CN110999788B CN110999788B (en) | 2021-08-24 |
Family
ID=70115193
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911268882.9A Active CN110999788B (en) | 2019-12-11 | 2019-12-11 | Method for rapidly propagating wintersweet plants |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110999788B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2613253A (en) * | 2021-11-03 | 2023-05-31 | Inst Botany Jiangsu Province & Cas | Preparation for improving survival rate of colored-leaf poplar and application thereof |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101352146A (en) * | 2008-09-11 | 2009-01-28 | 上海交通大学 | Quick breeding method for tissue culture of Geraldton wax |
| CN102599060A (en) * | 2012-03-29 | 2012-07-25 | 常熟市海虞茶叶有限公司 | Calyx canthus tissue culture rapid propagation method |
| EP2727997A1 (en) * | 2011-07-01 | 2014-05-07 | Shiseido Company, Ltd. | Plant cell differentiation promoter |
| CN104285791A (en) * | 2014-09-29 | 2015-01-21 | 中国计量学院 | Method applied to tissue culture and rapid propagation of chimonanthus nitens |
| CN104429508A (en) * | 2014-11-18 | 2015-03-25 | 北京林业大学 | Chimonanthus praecox cutting propagation method |
| CN105210587A (en) * | 2014-05-29 | 2016-01-06 | 天津盛优苗木种植专业合作社 | The cuttage planting method of a kind of wintersweet |
| CN106508907A (en) * | 2016-10-21 | 2017-03-22 | 全南厚朴生态林业有限公司 | Chimonanthus grammatus rooting agent formula and using method |
| CN107006370A (en) * | 2017-05-03 | 2017-08-04 | 中国科学院合肥物质科学研究院 | A kind of wax plum plant high-efficiency in-vitro regeneration method |
| US10440957B2 (en) * | 2016-04-08 | 2019-10-15 | Yibin Yunchen Abor & Gargen Co., Ltd. | Rooting agent for woody plants cutting |
-
2019
- 2019-12-11 CN CN201911268882.9A patent/CN110999788B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101352146A (en) * | 2008-09-11 | 2009-01-28 | 上海交通大学 | Quick breeding method for tissue culture of Geraldton wax |
| EP2727997A1 (en) * | 2011-07-01 | 2014-05-07 | Shiseido Company, Ltd. | Plant cell differentiation promoter |
| CN102599060A (en) * | 2012-03-29 | 2012-07-25 | 常熟市海虞茶叶有限公司 | Calyx canthus tissue culture rapid propagation method |
| CN105210587A (en) * | 2014-05-29 | 2016-01-06 | 天津盛优苗木种植专业合作社 | The cuttage planting method of a kind of wintersweet |
| CN104285791A (en) * | 2014-09-29 | 2015-01-21 | 中国计量学院 | Method applied to tissue culture and rapid propagation of chimonanthus nitens |
| CN104429508A (en) * | 2014-11-18 | 2015-03-25 | 北京林业大学 | Chimonanthus praecox cutting propagation method |
| US10440957B2 (en) * | 2016-04-08 | 2019-10-15 | Yibin Yunchen Abor & Gargen Co., Ltd. | Rooting agent for woody plants cutting |
| CN106508907A (en) * | 2016-10-21 | 2017-03-22 | 全南厚朴生态林业有限公司 | Chimonanthus grammatus rooting agent formula and using method |
| CN107006370A (en) * | 2017-05-03 | 2017-08-04 | 中国科学院合肥物质科学研究院 | A kind of wax plum plant high-efficiency in-vitro regeneration method |
Non-Patent Citations (4)
| Title |
|---|
| B. KOZOMARA等: "In vitro propagation of Chimonanthus praecox (L.), a winter", 《IN VITRO CELLULAR AND DEVELOPMENTAL BIOLOGY - PLANT》 * |
| MINGXIAO ZHAO等: "Establishment of in vitro plant regeneration system for Chimonanthus praecox (L.)", 《AFRICAN JOURNAL OF BIOTECHNOLOGY》 * |
| 张林等: "腊梅组织培养和植株再生", 《植物生理学通讯》 * |
| 袁晓琴等: "蜡梅扦插繁殖技术研究", 《现代农业科技》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2613253A (en) * | 2021-11-03 | 2023-05-31 | Inst Botany Jiangsu Province & Cas | Preparation for improving survival rate of colored-leaf poplar and application thereof |
| GB2613253B (en) * | 2021-11-03 | 2024-01-31 | Inst Botany Jiangsu Province & Cas | Preparation for improving survival rate of colored-leaf poplar and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110999788B (en) | 2021-08-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100998311B (en) | Method for artificially breeding edulis mono-garlic clove orchid | |
| CN104012417B (en) | High-efficiency and rapid micropropagation method for toxicodendron vernicifluum | |
| CN101297635B (en) | Method for breeding spore of Dryopteris varia | |
| CN102612961A (en) | Method for cultivating olive seedlings | |
| CN105191792B (en) | The rapid propagation method of almond ringdove chrysanthemum | |
| CN103314765A (en) | Method for reproducing magnolia zenii seedlings | |
| CN112273231A (en) | Method for inducing proliferation of asparagus cochinchinensis cluster buds and plant regeneration | |
| CN103039366B (en) | Industrial production method of mycorrhizal seedlings of Changbai Mountain rhododendron plants | |
| JPS5914725A (en) | Production of plant propagating material | |
| CN111657151A (en) | Rapid seedling method for acer truncatum | |
| CN107980634A (en) | A kind of method for obtaining the suitable explant of lucid asparagus tissue-culturing rapid propagation | |
| CN112438201A (en) | Tissue culture rapid propagation method of wine grapes | |
| CN110999788B (en) | Method for rapidly propagating wintersweet plants | |
| CN110771365B (en) | Cutting propagation method of lingbao rhododendron | |
| CN106613993B (en) | A kind of cultural method of the tissue cultures regrowth of trifoliate orange | |
| CN112655553A (en) | Rapid sterile short-shoot propagation method for Orthosiphon aristatus | |
| CN102217538A (en) | Method for treating walnut tissue culture stem segments inside test tubes and rooting outside test tubes | |
| CN110999782A (en) | Tissue culture and rapid propagation method for gardenia jasminoides | |
| CN114365691B (en) | Tissue culture method of fortune purple maple | |
| CN112243860B (en) | Tissue culture and rapid propagation method for Chinese parasol trees | |
| Shan et al. | Semi-sterilized tissue culture for rapid propagation of grapevines (Vitis vinifera L.) using immature cuttings | |
| CN110720393B (en) | Method for tissue culture and rapid propagation of ficus microcarpa | |
| CN109548655B (en) | Tissue culture method of quanlang tree | |
| CN107889745B (en) | Tissue culture method for preventing fir test-tube plantlet from deviating from crown | |
| CN107242138B (en) | Tissue culture rapid propagation and cultivation method of paphiopedilum chianus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |