CN110982675B - Microorganism enrichment breeding device - Google Patents
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- CN110982675B CN110982675B CN201911338190.7A CN201911338190A CN110982675B CN 110982675 B CN110982675 B CN 110982675B CN 201911338190 A CN201911338190 A CN 201911338190A CN 110982675 B CN110982675 B CN 110982675B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
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Abstract
The invention discloses a microorganism enrichment breeding device, which relates to the technical field of biological culture and comprises a liquid storage tank and an enrichment tank, wherein a filter cylinder and an adsorption stirring cylinder are arranged inside the enrichment tank, the adsorption stirring cylinder is positioned inside the filter cylinder, a culture solution in the liquid storage tank is conveyed into the adsorption filter cylinder, the adsorption filter cylinder consists of a fixed mounting part, an adsorption part and a stirring part, the stirring part can rotate inside the filter cylinder and comprises a stirring cylinder and stirring blades, the stirring blades are spirally mounted on the outer side surface of the stirring cylinder, when the filtering membrane in the filter cylinder is blocked, the culture solution starts to flow at an accelerated speed, and the flowing culture solution drives the stirring blades to rotate, so that microorganisms on the filtering membrane are scraped off, and the problem of blocking is solved. According to the microorganism enrichment propagator, the microorganism is scraped down by the mode that the culture solution flows to drive the stirring blade to rotate, so that the problem that a filtering membrane is easy to block is well solved, the whole filtering process does not need manual monitoring, energy is not consumed, and the microorganism enrichment propagator has a good application prospect.
Description
Technical Field
The invention relates to the technical field of biological culture, in particular to a microorganism enrichment propagator.
Background
Microorganism enrichment is a biological culture means for the purpose of vigorous propagation of specific microorganisms and ultimately separation and pure culture of microorganisms, and has been widely used in the fields of microbial research, medical research, and the like.
Common methods in the process of microorganism enrichment and propagation include a filtration method, a centrifugation method and the like, wherein the filtration method has low cost and high efficiency, so the method is most widely applied. However, the filtration method requires the use of a filtration membrane to filter the microorganisms to achieve the enrichment effect, the pore size of the filtration membrane is usually 22 μm or 45 μm, the filtration pores are very easy to be blocked during the filtration process, and the filtration efficiency is greatly reduced once the blockage occurs.
Disclosure of Invention
The embodiment of the invention provides a microorganism enrichment propagator, which can solve the problems in the prior art.
The invention provides a microorganism enrichment breeding device, which comprises at least one stage of enrichment breeding equipment, wherein each stage of enrichment breeding equipment comprises a liquid storage tank and an enrichment tank, a filter cylinder and an adsorption stirring cylinder are arranged in the enrichment tank, the adsorption stirring cylinder is positioned in the filter cylinder, a culture solution stored in the liquid storage tank is conveyed to the interior of the adsorption stirring cylinder through a pipeline, the filter cylinder is communicated into the liquid storage tank through a pipeline, a pressure release valve is arranged on the pipeline, the pressure release valve is provided with a liquid inlet pipe and a liquid outlet pipe, the liquid inlet pipe is communicated with the upper end of the side surface of the filter cylinder, and the liquid outlet pipe is communicated with the liquid storage tank;
the adsorption mixing drum comprises a fixed mounting part, an adsorption part and a mixing part, the fixed mounting part is mounted on the inner top surface of the filter drum, the adsorption part is mounted at the lower end of the fixed mounting part, the mixing part comprises a mixing drum and mixing blades, the mixing drum is rotatably mounted at the outer side surface of the adsorption part, the mixing blades are fixedly mounted on the outer side surface of the mixing drum in a spiral shape, the bottom end of the mixing drum is close to the inner bottom surface of the filter drum, the adsorption part is internally filled with an adsorption material for adsorbing microorganisms, the bottom end of the adsorption part is close to the inner bottom surface of the mixing drum, the pipeline of the liquid storage tank is extended into the adsorption part after passing through the fixed mounting part, and the bottom end of the pipeline is close to the inner bottom surface of the adsorption part.
The invention provides a microorganism enrichment breeding device, which comprises a liquid storage tank and an enrichment tank, wherein a filter cylinder and an adsorption stirring cylinder are arranged in the enrichment tank, the adsorption stirring cylinder is positioned in the filter cylinder, a culture solution in the liquid storage tank is conveyed into the adsorption filter cylinder, the adsorption filter cylinder consists of a fixed mounting part, an adsorption part and a stirring part, the stirring part can rotate in the filter cylinder and comprises a stirring cylinder and stirring blades, the stirring blades are spirally mounted on the outer side surface of the stirring cylinder, when the filter membrane in the filter cylinder is blocked, the culture solution starts to flow at an accelerated speed, and the flowing culture solution drives the stirring blades to rotate, so that microorganisms on the filter membrane are scraped off, and the problem of blocking is solved. According to the microorganism enrichment propagator, the microorganism is scraped down by the mode that the culture solution flows to drive the stirring blade to rotate, so that the problem that a filtering membrane is easy to block is well solved, the whole filtering process does not need manual monitoring, the energy is not consumed, the structure is simple, and the microorganism enrichment propagator has a good application prospect.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic view of a microorganism enrichment propagator provided by an embodiment of the invention;
FIG. 2 is a schematic structural diagram of a pressure relief valve;
FIG. 3 is a schematic longitudinal cross-sectional view of the pressure relief valve of FIG. 2;
FIG. 4 is a schematic structural view of an adsorption mixing drum;
FIG. 5 is a longitudinal sectional view of the adsorption agitation drum shown in FIG. 4.
Description of reference numerals:
100-liquid storage tank, 200-enrichment tank, 210-filter cartridge, 300-adsorption mixing drum, 311-top mounting plate, 312-connecting pipe, 313-bottom mounting plate, 320-mixing drum, 321-mounting ring, 322-adsorption shell, 330-stirring blade, 340-liquid injection pipe, 400-sterilization device, 500-valve body, 501-pressure relief cavity, 510-liquid inlet pipe, 520-liquid outlet pipe, 530-adjusting rod, 540-pressure relief rod, 541-pressure relief spring.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
Referring to fig. 1, an embodiment of the present invention provides a microorganism enrichment breeding device, including at least one stage of enrichment breeding equipment, each stage of enrichment breeding equipment includes a liquid storage tank 100 and an enrichment tank 200, a filter cartridge 210 and an adsorption mixing drum 300 are installed inside the enrichment tank 200, the adsorption mixing drum 300 is located inside the filter cartridge 210, a culture solution stored in the liquid storage tank 100 is delivered to the adsorption mixing drum 300 through a pipeline and a pump, a microbial flora is inoculated in the adsorption mixing drum 300, microorganisms grow and breed in a culture solution environment, and after being filtered by the filter cartridge 210, the enrichment tank 200 outputs an almost pure culture solution. When the enrichment propagation equipment has two or more stages, the culture solution output by the enrichment tank is conveyed to the liquid storage tank of the next stage, and the culture solution is conveyed to the liquid storage tank of the first stage by the enrichment tank of the last stage. If microbial flora inoculated in the multi-stage enrichment propagation equipment is different, the culture solution output by the enrichment tank of the previous stage is sterilized by the sterilization device 400 and then is conveyed to the liquid storage tank of the next stage, so that microbial pollution is avoided, and the culture solution output by the enrichment tank of the last stage is sterilized by the sterilization device and then is conveyed to the liquid storage tank of the first stage, so that the cyclic utilization of the culture solution is realized.
The medial surface of cartridge filter 210 is paved with filtration membrane, works as the filtration pore on the filtration membrane blocks up the back, culture solution pressure in the cartridge filter 210 can increase, in order to avoid the accident that takes place the equipment damage, the cartridge filter 210 still communicates to the liquid storage pot 100 in through the pipeline, installs the relief valve on this pipeline, works as when pressure in the cartridge filter 210 is too big, the relief valve is opened, carries excessive culture solution to the liquid storage pot 100 in, and when pressure in the cartridge filter 210 is normal, the relief valve is closed, makes the inside culture solution of cartridge filter 210 possess certain pressure, maintains the normal clear of filtering process.
Referring to fig. 2 and 3 together, the pressure relief valve includes a valve body 500, and a liquid inlet pipe 510, a liquid outlet pipe 520, and an adjusting rod 530 mounted on the valve body 500, where the liquid inlet pipe 510 and the liquid outlet pipe 520 are respectively mounted on two adjacent sides of the exterior of the valve body 500, and the adjusting rod 530 is mounted on a side of the exterior of the valve body 500 opposite to the liquid inlet pipe 510.
The cavity inside the valve body 500 is a pressure relief cavity 501, and the liquid inlet pipe 510 and the liquid outlet pipe 520 are both communicated with the pressure relief cavity 501. The pressure release cavity 501 is provided with a jack on the side facing the adjusting rod 530, the inner side of the jack is provided with internal threads, the side of the adjusting rod 530 is provided with external threads matching with the internal threads, the adjusting rod 530 is screwed in the jack, and one end of the adjusting rod extends into the pressure release cavity 501. The pressure release rod 540 with an inverted T shape is installed in the pressure release cavity 501, the upper end of the pressure release rod 540 is inserted into the adjusting rod 530 in a sliding manner, the pressure release rod 540 is sleeved with a pressure release spring 541, two ends of the pressure release spring 541 are respectively abutted against the lower end of the adjusting rod 530 and the lower end of the pressure release rod 540, the lower end of the pressure release rod 540 is positioned right above the liquid inlet pipe 510, and the sectional area of the lower end of the pressure release rod 540 is larger than that of the liquid inlet pipe 510.
The liquid inlet pipe 510 is communicated with the upper end of the side surface of the filter cartridge 210, the liquid outlet pipe 520 is communicated with the liquid storage tank 100, when the pressure of the culture liquid in the filter cartridge 210 is too large and is larger than the elastic force of the pressure relief spring 541, the pressure relief rod 540 is pushed away, and the culture liquid flows into the pressure relief cavity 501 and then flows out of the liquid outlet pipe 520 to enter the liquid storage tank 100. The length of the adjusting rod 530 extending into the pressure release cavity 501 can be adjusted by rotating the adjusting rod, so that the elastic force of the pressure release spring 541 is adjusted, and the opening pressure of the pressure release valve can be adjusted as required.
Refer to fig. 4 and 5 in the lump, absorption churn 300 includes fixed mounting portion, absorption portion and stirring portion, fixed mounting portion installs on the interior top surface of cartridge filter 210, the lower extreme at fixed mounting portion is installed to the absorption portion, stirring portion rotates and installs on the lateral surface of absorption portion, stirring portion includes churn 320 and stirring vane 330, a large amount of through-holes have been seted up on the side of agitator 320, make inside culture solution can permeate out, the spiral helicine fixing of stirring vane 330 is on the lateral surface of agitator 320. When filtration membrane in the filter cartridge 210 blocks up, the culture fluid pressure increase in the filter cartridge 210, after the relief valve was opened, the culture solution in the filter cartridge 210 flows out through the relief valve, and stirring vane 330 is driven to rotate when mobile culture solution passes through stirring vane 330, the distance between stirring vane 330 and the filtration membrane is very little, consequently can scrape the microorganism of gathering on filtration membrane down, solves the problem of jam. After the problem of jam is solved, the culture fluid pressure in the filter cartridge 210 reduces, and the relief valve is closed, and the culture fluid flows out through filtration membrane, and stirring vane 330 stops or stops the rotation basically, repeats above process once more when next emergence is blockked up, makes filtration membrane is in normal filtration state constantly, improves filtration efficiency.
In this embodiment, the fixed mounting portion is i-shaped, and is composed of a top mounting disk 311, a connecting pipe 312 and a bottom mounting disk 313, the upper end of the connecting pipe 312 is fixedly mounted at the center of the bottom surface of the top mounting disk 311, the lower end of the connecting pipe 312 is fixedly mounted at the center of the top surface of the bottom mounting disk 313, the centers of the top mounting disk 311 and the bottom mounting disk 313 are both provided with holes, and the upper end and the lower end of the connecting pipe 312 are respectively communicated with the holes on the top mounting disk 311 and the bottom mounting disk 313.
Adsorption part includes collar 321 and adsorption shell 322, the bottom in bottom mounting disc 313 side is installed to the upper end detachable of collar 321 medial surface, and the mode of dismantling in this embodiment is the spiro union. The upper end of churn 320 rotates and cup joints the lower extreme at collar 321, the quantity of adsorbing shell 322 is two, and range upon range of formula fixed mounting from top to bottom the medial surface lower extreme of collar 321, and two-layer it has the certain distance to adsorb between the shell 322, two it has the adsorbing material that is used for adsorbing the microorganism to adsorb the intracavity packing that forms between the shell 322, for example cellosilk, porous ceramic etc. and two-layer it has all seted up a large amount of through-holes on the shell 322 to adsorb, makes things convenient for the culture solution to flow out.
Annotate liquid pipe 340 and pass and stretch into to two-layer behind top mounting disc 311, connecting pipe 312 and the bottom mounting disc 313 in the top of the adsorption shell that is in the top in the adsorption shell 322, just it is very little with the distance of adsorption shell 322 to annotate liquid pipe 340, is in the below simultaneously the interior bottom surface distance of adsorption shell 322 and churn 320 is very little, makes the culture solution of liquid storage pot 100 output can directly flow into the position that is close to cartridge filter 210 bottom surface through annotating liquid pipe 340. When the filtering membrane is blocked, the culture solution flowing out from the liquid injection pipe 340 needs to flow upwards in the filter cartridge 210 to enter the pressure release valve, so that the culture solution drives the stirring blade 330 to rotate in the process of flowing upwards from bottom to top, and the effect of scraping microorganisms by the stirring blade is improved.
The top mounting plate 311 in the fixed mounting part is detachably mounted on the filter cartridge 210, specifically, a mounting hole is formed in the top surface of the filter cartridge 210, after the adsorption stirring cylinder 300 passes through the mounting hole and extends into the filter cartridge 210, the top mounting plate 311 is mounted in the mounting hole, and it should be noted that the diameter of the top mounting plate 311 is the largest in all structures of the adsorption stirring cylinder 300, so that obstruction in the mounting and dismounting processes is avoided.
Because annotate liquid pipe 340 and fixed mounting portion body coupling, consequently whole it is very convenient that adsorption filtration cartridge 300 is installed, can wholly install and dismantle, does not need long distance plug pipeline. And the adsorption part is detachably arranged on the fixed installation part, the detachment is also very convenient, and the adsorption part can be directly detached and washed after the enrichment and propagation operation is finished to obtain the needed microorganisms. Meanwhile, the stirring part rotates and the adsorption part does not rotate in the filtering process, so that microorganisms in the adsorption part are in a relatively static liquid environment, and the propagation of the microorganisms is facilitated.
The above disclosure is only for a few specific embodiments of the present invention, however, the present invention is not limited to the above embodiments, and any modifications that can be made by those skilled in the art are intended to fall within the scope of the present invention.
Claims (6)
1. The microorganism enrichment breeding device is characterized by comprising at least one stage of enrichment breeding equipment, wherein each stage of enrichment breeding equipment comprises a liquid storage tank and an enrichment tank, a filter cylinder and an adsorption stirring cylinder are arranged inside the enrichment tank, the adsorption stirring cylinder is positioned inside the filter cylinder, culture solution stored in the liquid storage tank is conveyed to the inside of the adsorption stirring cylinder through a pipeline, the filter cylinder is communicated into the liquid storage tank through a pipeline, a pressure release valve is arranged on the pipeline, the pressure release valve is provided with a liquid inlet pipe and a liquid outlet pipe, the liquid inlet pipe is communicated with the upper end of the side surface of the filter cylinder, and the liquid outlet pipe is communicated with the liquid storage tank;
the adsorption mixing drum comprises a fixed mounting part, an adsorption part and a mixing part, the fixed mounting part is mounted on the inner top surface of the filter drum, the adsorption part is mounted at the lower end of the fixed mounting part, the mixing part comprises a mixing drum and a mixing blade, the mixing drum is rotatably mounted on the outer side surface of the adsorption part, the mixing blade is fixedly mounted on the outer side surface of the mixing drum in a spiral shape, the bottom end of the mixing drum is close to the inner bottom surface of the filter drum, an adsorption material for adsorbing microorganisms is filled in the adsorption part, the bottom end of the adsorption part is close to the inner bottom surface of the mixing drum, a pipeline communicated with the liquid storage tank penetrates through the fixed mounting part and then extends into the adsorption part, and the bottom end of the pipeline is close to the inner bottom surface of the adsorption part;
the enrichment propagation equipment is provided with two or more stages, the culture solution output by the enrichment tank is conveyed to the next-stage liquid storage tank, and the culture solution is conveyed to the first-stage liquid storage tank by the last-stage enrichment tank;
microbial flora is inoculated in the adsorption mixing drum, when microbes inoculated in the multi-stage enrichment propagation equipment are different, the culture solution output by the enrichment tank of the previous stage is sterilized by a sterilization device and then is conveyed to the liquid storage tank of the next stage, and meanwhile, the culture solution output by the enrichment tank of the last stage is sterilized by the sterilization device and then is conveyed to the liquid storage tank of the first stage;
and a filtering membrane is paved on the inner side surface of the filtering cylinder.
2. The microorganism enrichment and propagation device according to claim 1, wherein the pressure release valve comprises a valve body and an adjusting rod, the liquid inlet pipe and the liquid outlet pipe are respectively arranged on two adjacent sides of the outside of the valve body, and the adjusting rod is arranged on the side of the outside of the valve body opposite to the liquid inlet pipe;
the inside cavity of valve body is the pressure release chamber, feed liquor pipe and drain pipe all communicate with this pressure release chamber, adjust the pole insert the pressure release intracavity portion and with the valve body spiro union, install the pressure release pole of the type of falling T in the pressure release chamber, the inside of adjusting the pole is inserted in the upper end slip of pressure release pole, pressure release spring has been cup jointed on the pressure release pole, pressure release spring's both ends support respectively and hold the lower extreme of adjusting the pole and the lower extreme of pressure release pole, pressure release pole's lower extreme is in directly over the feed liquor pipe, just pressure release pole's lower extreme sectional area is greater than the sectional area of feed liquor pipe.
3. The microorganism enrichment reproduction device of claim 1, wherein the fixed mounting part comprises a top mounting plate, a connecting pipe and a bottom mounting plate, the upper end of the connecting pipe is fixedly mounted in the center of the bottom surface of the top mounting plate, the lower end of the connecting pipe is fixedly mounted in the center of the top surface of the bottom mounting plate, the centers of the top mounting plate and the bottom mounting plate are provided with holes, and the upper end and the lower end of the connecting pipe are respectively communicated with the holes on the top mounting plate and the bottom mounting plate; the top mounting disc is detachably mounted on the top surface of the filter cylinder, and the liquid injection pipe penetrates through the top mounting disc, the connecting pipe and the bottom mounting disc and then extends into the adsorption part.
4. The microorganism enrichment and reproduction device as claimed in claim 3, wherein the filter cartridge has a mounting hole on the top surface, the adsorption mixing drum passes through the mounting hole and extends into the filter cartridge, and the top mounting plate is detachably mounted in the mounting hole.
5. The microorganism enrichment reproduction device of claim 3, wherein the adsorption part comprises a mounting ring and an adsorption shell, the upper end of the inner side of the mounting ring is detachably mounted at the bottom end of the side of the bottom mounting plate, and the upper end of the stirring cylinder is rotatably sleeved at the lower end of the mounting ring; the number of the adsorption shells is two, the adsorption shells are fixedly installed at the lower end of the inner side face of the mounting ring in an up-down stacked mode, an adsorption cavity is formed between the two layers of the adsorption shells, and the adsorption materials are filled in the adsorption cavity.
6. The microorganism enrichment propagator of claim 5, wherein the adsorbent material is a fiber filament or a porous ceramic.
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| CN201911338190.7A CN110982675B (en) | 2019-12-23 | 2019-12-23 | Microorganism enrichment breeding device |
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| CN201911338190.7A CN110982675B (en) | 2019-12-23 | 2019-12-23 | Microorganism enrichment breeding device |
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|---|---|---|---|---|
| JPH0595778A (en) * | 1991-10-07 | 1993-04-20 | Agency Of Ind Science & Technol | Porous separation membrane integrated type incubator equipped with stirrer |
| JPH08280391A (en) * | 1995-04-18 | 1996-10-29 | Kao Corp | Recovery of fermentation product |
| JP2008043911A (en) * | 2006-08-21 | 2008-02-28 | Kurita Water Ind Ltd | Sludge thickening apparatus |
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| JP2018161115A (en) * | 2017-03-27 | 2018-10-18 | 株式会社日立製作所 | Cell culture apparatus and cell culture method |
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