CN110980967A - Microbial composite water purifying agent and preparation method thereof - Google Patents
Microbial composite water purifying agent and preparation method thereof Download PDFInfo
- Publication number
- CN110980967A CN110980967A CN201911328007.5A CN201911328007A CN110980967A CN 110980967 A CN110980967 A CN 110980967A CN 201911328007 A CN201911328007 A CN 201911328007A CN 110980967 A CN110980967 A CN 110980967A
- Authority
- CN
- China
- Prior art keywords
- purifying agent
- water purifying
- microbial
- composite
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention relates to a microbial compound water purifying agent, which consists of a compound microbial preparation and a water purifying agent compound carrier; the compound microbial preparation comprises bacillus subtilis, bacillus amyloliquefaciens, lactobacillus acidophilus, bacillus coagulans, bacillus licheniformis and pseudomonas aeruginosa; the water purifying agent composite carrier comprises polyacrylamide, polyaluminum ferric chloride, polyether quaternary ammonium salt, diatom ooze, coconut shell carbon, chitosan, sulfonated coal, zeolite, clay and hydroxyapatite. The microbial composite water purifying agent adopts indigenous microorganisms in river and lake water bodies and bottom mud, has rich resources, is easy to obtain and has low cost; the indigenous microorganisms after the expanded culture are rapidly propagated, and the synergistic water purifying agent can degrade pollutants in water in large quantity, so that a carbon source is converted into carbon dioxide and a nitrogen source is converted into nitrogen, the concentration of the pollutants is greatly reduced, and the water body becomes clear.
Description
Technical Field
The invention belongs to the technical field of sewage treatment, and relates to a microbial composite water purifying agent and a preparation method thereof.
Background
At present, the existence of acrylamide is difficult to avoid in the synthesis of organic polymer water purifying agents, and the acrylamide is a highly toxic substance and causes harm to human health. In the present day, a new water purifying agent is urgently sought for replacing the traditional water purifying agent, and the microbial water purifying agent has a plurality of unique properties and advantages: degradable, non-toxic, harmless, high safety and no secondary pollution. Therefore, the microbial water purifying agent has been regarded and accepted by many researchers as a high-efficiency safe water purifying agent, but the existing microbial water purifying agent has the following defects: large dosage, unstable effect, undefined strain source and limited use condition, and can not meet all sewage treatment requirements.
Disclosure of Invention
In order to solve the technical problems, the invention aims to provide a microbial composite water purifying agent and a preparation method thereof.
Based on the above purpose, the invention adopts the technical scheme that:
a microbial composite water purifying agent consists of a composite microbial preparation and a water purifying agent composite carrier, wherein the composite microbial preparation comprises one or more of bacillus subtilis, bacillus amyloliquefaciens, lactobacillus acidophilus, bacillus coagulans, bacillus licheniformis and pseudomonas aeruginosa;
the water purifying agent composite carrier comprises one or more of polyacrylamide, polyaluminum ferric chloride, polyether quaternary ammonium salt, diatom ooze, coconut shell carbon, chitosan, sulfonated coal, zeolite, clay and hydroxyapatite.
Preferably, the ratio of the wet thallus weight of the compound microbial preparation to the dry weight of the water purifying agent compound carrier is 2-3: 3-5.
Preferably, the ratio of viable count of bacillus subtilis, bacillus amyloliquefaciens, lactobacillus acidophilus, bacillus coagulans, bacillus licheniformis and pseudomonas aeruginosa in the composite microbial preparation is 3-4: 3-4: 2-3: 2-3: 1-2: 2-3.
Preferably, the mass ratio of polyacrylamide, polyaluminum ferric chloride, polyether quaternary ammonium salt, diatom ooze, coconut shell carbon, chitosan, sulfonated coal, zeolite, clay and hydroxyapatite in the water purifying agent composite carrier is 10-20: 5-15: 10-20: 15-25: 10-25: 10-25: 10-25: 5-15: 5-15: 1-5.
Preferably, the water content of the microbial composite water purifying agent is 30-50%.
The invention provides a preparation method of a compound microbial preparation, which comprises the following steps:
collecting a river and lake water sample and bottom mud, centrifuging, and inoculating the supernatant into a culture medium for culture to obtain a water body bacterial liquid;
taking water body bacteria liquid for gradient dilution, then coating the water body bacteria liquid on a solid culture medium for culture, separating to obtain a single colony, and identifying and screening the single colony;
respectively carrying out amplification culture on the screened bacillus subtilis, bacillus amyloliquefaciens, lactobacillus acidophilus, bacillus coagulans, bacillus licheniformis and pseudomonas aeruginosa, and then mixing the strains to obtain the composite microbial preparation.
Preferably, collecting a river and lake water sample and bottom mud, centrifuging, inoculating the supernatant into an NB nutrient broth culture medium, and culturing at 25-32 ℃ for 12-72h to obtain a water body bacterial liquid.
Preferably, the water bacterial liquid is subjected to gradient dilution, then the water bacterial liquid is coated on an NB nutrient broth solid culture medium, the NB nutrient broth solid culture medium is cultured for 12-72h at 25-32 ℃, a single colony is separated, and the single colony obtained by separation is identified and screened according to the physiological and biochemical characteristics of bacteria.
More preferably, the single colony obtained by separation is identified and screened by using a DNA detection technology; or, the single colony obtained by separation is identified and screened according to the physiological and biochemical characteristics of the bacteria and the DNA detection technology.
Preferably, the incubation time is such that the species is grown to a point where it can be isolated.
Preferably, the bacillus subtilis, the bacillus amyloliquefaciens, the lactobacillus acidophilus, the bacillus coagulans, the bacillus licheniformis and the pseudomonas aeruginosa obtained by screening are respectively subjected to amplification culture, and then the ratio of bacillus subtilis to bacillus amyloliquefaciens to lactobacillus acidophilus to bacillus coagulans to bacillus licheniformis and pseudomonas aeruginosa is calculated according to the following formula (3-4): 3-4: 2-3: 2-3: 1-2: 2-3 of the viable count ratio, and mixing the strains to obtain the compound microbial preparation.
Preferably, the total viable bacteria concentration of the obtained composite microbial preparation is 8-20×108CFU/g。
Specifically, the preparation method of the compound microbial preparation comprises the following steps:
(1) collecting a river and lake water sample and bottom mud, centrifuging, inoculating a supernatant into an NB nutrient broth culture medium, and culturing at 25-32 ℃ for 12-72h to obtain a water body bacterial liquid;
(2) taking water body bacteria liquid for gradient dilution, then coating the water body bacteria liquid on an NB nutrient broth solid culture medium, culturing for 12-72h at 25-32 ℃, separating to obtain a single colony, and identifying and screening the single colony obtained by separation according to the physiological and biochemical characteristics of bacteria.
(3) Respectively carrying out amplification culture on the screened bacillus subtilis, bacillus amyloliquefaciens, lactobacillus acidophilus, bacillus coagulans, bacillus licheniformis and pseudomonas aeruginosa, and then carrying out amplification culture on the bacillus subtilis, the bacillus amyloliquefaciens, the lactobacillus acidophilus, the bacillus coagulans, the bacillus licheniformis and the pseudomonas aeruginosa according to the ratio of 3-4: 3-4: 2-3: 2-3: 1-2: 2-3 of the viable count ratio, and mixing the strains to obtain the compound microbial preparation.
The invention also provides a preparation method of the water purifying agent composite carrier, which comprises the following steps:
mixing polyacrylamide, polyaluminum ferric chloride, polyether quaternary ammonium salt, sulfonated coal, zeolite, coconut shell carbon, chitosan and a certain amount of water, heating, stirring, adding diatom ooze, clay and hydroxyapatite, stirring, and filtering to obtain a solid substance;
and drying the solid matter, and granulating to obtain the water purifying agent composite carrier.
Preferably, the amount of water is sufficient to soak the other components and to allow stirring, and the amount of water may be increased accordingly during stirring, e.g., by increasing the components.
Preferably, the mass ratio of polyacrylamide, polyaluminum ferric chloride, polyether quaternary ammonium salt, diatom ooze, coconut shell carbon, chitosan, sulfonated coal, zeolite, clay and hydroxyapatite in the water purifying agent composite carrier is 10-20: 5-15: 10-20: 15-25: 10-25: 10-25: 10-25: 5-15: 5-15: 1-5.
Preferably, polyacrylamide, polymeric aluminum ferric chloride, polyether quaternary ammonium salt, sulfonated coal, zeolite, coconut shell carbon, chitosan and a certain amount of water are mixed, heated to 40-55 ℃, stirred for 4-6h at the rotation speed of 200-350rpm, then added with diatom ooze, clay and hydroxyapatite in the stirring state, continuously stirred for 1-2h, and then filtered to obtain a solid matter.
Preferably, the solid substance is dried at 45-80 ℃ and granulated to obtain the water purifying agent composite carrier.
More preferably, the solid substance is dried at 45-80 ℃ until the water content is 30% -75%, and granulated to obtain the water purifying agent composite carrier. Further preferably, granulating by using a throwing granulator to obtain the water purifying agent composite carrier with the particle size of 1-6 mm; more preferably 3-5 mm.
Preferably, the water purifying agent composite carrier is dried at the temperature of 45-80 ℃ until the water content is 10% -30%. Under the condition that the water content is 10% -30%, the water purifying agent composite carrier is not easy to disperse or fall off powder, and the culture medium is favorably added in the subsequent steps.
Specifically, the preparation method of the water purifying agent composite carrier comprises the following steps:
(1) mixing polyacrylamide, polymeric aluminum ferric chloride, polyether quaternary ammonium salt, sulfonated coal, zeolite, coconut shell carbon, chitosan and a certain amount of water according to the mass ratio, heating to 40-55 ℃, stirring for 4-6h at the rotation speed of 200-350rpm, then adding diatom ooze, clay and hydroxyapatite in the stirring state, continuing stirring for 1-2h, and then filtering to obtain a solid substance;
(2) drying the solid substance at 45-80 deg.C until the water content is 30% -75%, granulating with a throwing-round granulator to obtain water purifying agent composite carrier with particle size of 1-6mm, and drying until the water content is 10% -30%.
The invention also provides a preparation method of the microbial composite water purifying agent, which comprises the following steps:
centrifuging the compound microbial preparation, and removing supernatant to obtain wet thalli;
taking the wet bacteria, carrying out heavy suspension by using a proper amount of culture medium, adding the water purifying agent composite carrier, and culturing to obtain the microbial composite water purifying agent.
Preferably, the culture temperature is 20-32 deg.C, and the culture is performed at 50-300rpm for 12-24 h. More preferably, the rotation speed is preferably such that the water purifying agent composite carrier can be suspended with slight shaking, thereby facilitating the adhesion of microorganisms to the water purifying agent composite carrier.
Preferably, the culture medium is fresh medium; more preferably NB nutrient broth.
Preferably, a certain amount of fresh culture medium is used for suspending wet thalli to obtain a suspension bacterial liquid, the fresh culture medium is added to be 1-3 times of the volume of the culture medium before centrifugation, and the water purifying agent composite carrier is added.
Optionally, a certain amount of fresh culture medium is added into the water purifying agent composite carrier, then the fresh culture medium is added into the suspension liquid, and then the fresh culture medium is supplemented to be 1-3 times of the volume of the culture medium before centrifugation.
Preferably, the obtained microbial compound water purifying agent is fished out or filtered and dried. More preferably, drying at 15-32 ℃; drying to water content of 30-50%. Under the condition that the water content is 30-50%, the microbial composite water purifying agent is not easy to scatter or fall off, is beneficial to sewage treatment and secondary pollution avoidance, and more importantly, the requirements of strain survival rate and storage and transportation can be met to the maximum extent at the water content.
Preferably, the weight ratio of the wet bacteria in the obtained microbial composite water purifying agent to the dry weight of the water purifying agent composite carrier is controlled to be 2-3: 3-5. Note that the amount of wet cells was slightly increased from the theoretical value.
Specifically, the preparation method of the microbial composite water purifying agent comprises the following steps:
(1) centrifuging the compound microbial preparation, and removing supernatant to obtain wet thalli;
(2) taking the wet bacteria, carrying out heavy suspension on the wet bacteria by using a proper amount of NB nutrient broth culture medium to obtain suspension bacteria, and adding a fresh culture medium to the volume which is 1-3 times that of the culture medium before centrifugation;
(3) adding water purifying agent composite carrier, culturing at 20-32 deg.C and 50-300rpm for 12-24 hr to obtain microorganism composite water purifying agent, drying at 15-32 deg.C, and drying to water content of 30% -50%.
Optionally, the preparation method of the microbial composite water purifying agent comprises the following steps:
(1) centrifuging the compound microbial preparation, and removing supernatant to obtain wet thalli;
(2) taking the wet bacteria, and carrying out heavy suspension on the wet bacteria by using a proper amount of NB nutrient broth culture medium to obtain suspension bacteria liquid;
(3) adding a certain amount of NB nutrient broth culture medium into the water purifying agent composite carrier, mixing with the suspension bacterial liquid obtained in the step (2), supplementing the NB nutrient broth culture medium to 1-3 times of the volume of the culture medium before centrifugation, culturing at the culture temperature of 20-32 ℃ and the rotation speed of 50-300rpm for 12-24h to obtain the microbial composite water purifying agent, drying at the temperature of 15-32 ℃ until the water content is 30% -50%.
The beneficial technical effects of the invention are as follows:
the microorganism in the microbial composite water purifying agent is indigenous microorganism collected in river and lake water bodies and bottom mud, and has the advantages of rich resources, easy acquisition and low cost. Putting the collected indigenous microorganisms into a biological culture medium for amplification culture, and rapidly propagating the indigenous microorganisms subjected to amplification culture; the indigenous microorganisms are attached and fixed in the water purifying agent composite carrier, so that sewage treatment is facilitated, secondary pollution is avoided, pollutants in water can be degraded in a large amount by the indigenous microorganisms and the water purifying agent, a carbon source is converted into carbon dioxide, a nitrogen source is converted into nitrogen, the concentration of the pollutants is greatly reduced, and the water body becomes clear. Through the reconstruction, the perfection and the optimization of the water body ecological system, the recovery of the underwater original ecological system is promoted, and the self-purification capacity of the water body is finally recovered.
Detailed Description
The invention will now be described in detail with reference to specific examples, which are intended to illustrate the invention but not to limit it further.
Example 1
A preparation method of a compound microbial preparation comprises the following steps:
(1) collecting a river and lake water sample and bottom mud, centrifuging, inoculating a supernatant into an NB nutrient broth culture medium, and culturing at 26 ℃ for 48h to obtain a water body bacterial liquid;
(2) taking water body bacteria liquid for gradient dilution, then coating the water body bacteria liquid on an NB nutrient broth solid culture medium, culturing for 12-72h at 26 ℃, separating to obtain a single colony, and identifying and screening the single colony obtained by separation according to the physiological and biochemical characteristics of bacteria.
(3) Respectively carrying out amplification culture on the screened bacillus subtilis, bacillus amyloliquefaciens, lactobacillus acidophilus, bacillus coagulans, bacillus licheniformis and pseudomonas aeruginosa, and then mixing the strains according to the viable count proportion to obtain the compound microbial preparation.
The composite microbial preparation is prepared from bacillus subtilis, bacillus amyloliquefaciens, lactobacillus acidophilus, bacillus coagulans, bacillus licheniformis and pseudomonas aeruginosa according to the viable count proportion of 3: 3: 2: 2: 2: 3, preparing; the obtained composite microbial preparation has total viable bacteria concentration of 16 × 108CFU/g。
A preparation method of a water purifying agent composite carrier comprises the following steps:
(1) mixing polyacrylamide, polyaluminum ferric chloride, polyether quaternary ammonium salt, sulfonated coal, zeolite, coconut shell carbon and chitosan according to a mass ratio with a certain amount of water, stirring for 4.5 hours at 45 ℃ and 280rpm, then adding diatom ooze, clay and hydroxyapatite under a stirring state, continuing stirring for 1.5 hours, and then filtering to obtain a solid substance;
(2) drying the solid matter at 60 ℃ until the water content is 55%, granulating by using a throwing circle granulator to obtain the water purifying agent composite carrier with the grain diameter of 3-5mm, and drying until the water content is 10% -30%.
In the water purifying agent composite carrier, the mass ratio of polyacrylamide, polyaluminum ferric chloride, polyether quaternary ammonium salt, diatom ooze, coconut shell carbon, chitosan, sulfonated coal, zeolite, clay and hydroxyapatite is 14: 8: 15: 18: 17: 18: 18: 7: 12: 3.
a preparation method of a microbial compound water purifying agent comprises the following steps:
(1) centrifuging the compound microbial preparation, and removing supernatant to obtain wet thalli;
(2) taking the wet bacteria, carrying out heavy suspension on the wet bacteria by using a proper amount of NB nutrient broth culture medium to obtain suspension bacteria, and adding a fresh culture medium to the volume which is 1.5 times that of the culture medium before centrifugation;
(3) adding water purifying agent composite carrier, culturing at 22 deg.C and 60rpm for 18h to obtain microorganism composite water purifying agent, drying at 20 deg.C with cold air dryer, and drying to water content of 30% -50%.
The weight ratio of the wet bacteria in the microbial composite water purifying agent to the dry weight of the water purifying agent composite carrier is about 1: 1.
example 2
A preparation method of a compound microbial preparation comprises the following steps:
(1) collecting a river and lake water sample and bottom mud, centrifuging, inoculating a supernatant into an NB nutrient broth culture medium, and culturing at 27 ℃ for 60h to obtain a water body bacterial liquid;
(2) taking water body bacteria liquid for gradient dilution, then coating the water body bacteria liquid on an NB nutrient broth solid culture medium, culturing for 12-72h at 27 ℃, separating to obtain single colonies, and identifying and screening the single colonies obtained by separation according to the physiological and biochemical characteristics of bacteria.
(3) Respectively carrying out amplification culture on the screened bacillus subtilis, bacillus amyloliquefaciens, lactobacillus acidophilus, bacillus coagulans, bacillus licheniformis and pseudomonas aeruginosa, and then mixing the strains according to the viable count proportion to obtain the compound microbial preparation. The composite microbial preparation is prepared from bacillus subtilis, bacillus amyloliquefaciens, lactobacillus acidophilus, bacillus coagulans, bacillus licheniformis and pseudomonas aeruginosa according to the viable count proportion of 4: 4: 2: 3: 2: 3, preparing; the obtained composite microbial preparation has total viable bacteria concentration of 11 × 108CFU/g。
A preparation method of a water purifying agent composite carrier comprises the following steps:
(1) mixing polyacrylamide, polyaluminum ferric chloride, polyether quaternary ammonium salt, sulfonated coal, zeolite, coconut shell carbon and chitosan with a certain amount of water according to a mass ratio, heating to 47 ℃, stirring for 6 hours at a rotation speed of 200rpm, then adding diatom ooze, clay and hydroxyapatite in a stirring state, continuing stirring for 2 hours, and then filtering to obtain a solid substance;
(2) drying the solid matter at 75 ℃ until the water content is 50%, granulating by using a throwing circle granulator to obtain the water purifying agent composite carrier with the grain diameter of 3-5mm, and drying until the water content is 10% -30%.
The mass ratio of polyacrylamide, polymeric aluminum ferric chloride, polyether quaternary ammonium salt, diatom ooze, coconut shell charcoal, chitosan, sulfonated coal, zeolite, clay and hydroxyapatite in the water purifying agent composite carrier is 13: 9: 13: 16: 16: 12: 16: 8: 14: 2.
a preparation method of a microbial compound water purifying agent comprises the following steps:
(1) centrifuging the compound microbial preparation, and removing supernatant to obtain wet thalli;
(2) taking the wet bacteria, and carrying out heavy suspension on the wet bacteria by using a proper amount of NB nutrient broth culture medium to obtain suspension bacteria liquid;
(3) adding a certain amount of NB nutrient broth culture medium into the water purifying agent composite carrier, mixing with the suspension bacterial liquid obtained in the step (2), supplementing the NB nutrient broth culture medium to 2 times of the volume of the culture medium before centrifugation, culturing at the culture temperature of 24 ℃ and the rotation speed of 100rpm for 16h to obtain the microbial composite water purifying agent, drying by using a cold air dryer at the temperature of 20 ℃, and drying until the water content is 30% -50%.
The weight ratio of the wet bacteria in the microbial composite water purifying agent to the dry weight of the water purifying agent composite carrier is about 3: 4.
comparative example 1
A commercial water purifying agent comprises the following components:
16 parts of sulfonated coal, 15 parts of diatom ooze, 0.5 part of aluminum sulfate, 2 parts of oxalic acid, 5 parts of sodium silicate, 8 parts of sodium chloride and 20 parts of water.
Example 3
The microbial composite water purifying agents prepared in examples 1 to 2 were subjected to a sewage treatment test with a commercial water purifying agent of a comparative example.
The specific experimental scheme is as follows: the microbial composite water purifying agent prepared in the embodiment 1-2 and a water purifying agent sold in a comparative example are respectively added into a sewage treatment tank according to 0.3g/L, meanwhile, a container type A-H composite river and lake water body purifying machine prepared by Guangdong Huixin environmental protection Co., Ltd is used for increasing oxygen for a water body, untreated river water and purified water after being treated for 8 hours in the sewage treatment tank are detected, and the results of various indexes are shown in Table 1.
TABLE 1 test results of sewage treatment
Note: the transparency is measured by adopting a Seattle disk method; the dissolved oxygen measurement adopts an iodometry method; the oxidation-reduction potential adopts a depolarization determination method; the ammonia nitrogen adopts a Nassner reagent colorimetric method.
As can be seen from Table 1, all indexes of the original untreated river water belong to the classification standard of the mild black and odorous water body, and the purified water treated by the microbial composite water purifying agent disclosed by the invention meets the C-class water quality standard of landscape entertainment water; the transparency, dissolved oxygen and oxidation-reduction potential of the purified water after the treatment of the embodiment 1-2 are obviously greater than those of the comparative example, and the ammonia nitrogen content of the purified water of the embodiment is obviously less than that of the comparative example.
The above examples are given for the purpose of illustrating the invention clearly and not for the purpose of limiting the same, and it will be apparent to those skilled in the art that, in light of the above description, numerous modifications and variations can be made in the form and details of the embodiments of the invention described herein, and it is not intended to be exhaustive or to limit the invention to the precise forms disclosed.
Claims (10)
1. The microbial compound water purifying agent is characterized by consisting of a compound microbial preparation and a water purifying agent compound carrier;
the compound microbial preparation comprises one or more of bacillus subtilis, bacillus amyloliquefaciens, lactobacillus acidophilus, bacillus coagulans, bacillus licheniformis and pseudomonas aeruginosa;
the water purifying agent composite carrier comprises one or more of polyacrylamide, polyaluminum ferric chloride, polyether quaternary ammonium salt, diatom ooze, coconut shell carbon, chitosan, sulfonated coal, zeolite, clay and hydroxyapatite.
2. The microbial composite water purifying agent according to claim 1, wherein the ratio of the wet bacterial weight of the composite microbial preparation to the dry weight of the composite carrier of the water purifying agent is 2-3: 3-5.
3. The microbial composite water purifying agent according to claim 1, wherein the ratio of viable count of bacillus subtilis, bacillus amyloliquefaciens, lactobacillus acidophilus, bacillus coagulans, bacillus licheniformis and pseudomonas aeruginosa in the composite microbial preparation is 3-4: 3-4: 2-3: 2-3: 1-2: 2-3.
4. The microbial composite water purifying agent of claim 1, wherein the mass ratio of polyacrylamide, polyaluminum ferric chloride, polyether quaternary ammonium salt, diatom ooze, coconut shell charcoal, chitosan, sulfonated coal, zeolite, clay and hydroxyapatite in the water purifying agent composite carrier is 10-20: 5-15: 10-20: 15-25: 10-25: 10-25: 10-25: 5-15: 5-15: 1-5.
5. The microbial composite water purifying agent according to claim 1, wherein the water content of the microbial composite water purifying agent is 30-50%.
6. The microbial composite water purifying agent according to claim 1, wherein the preparation method of the composite microbial preparation comprises the following steps:
collecting a river and lake water sample and bottom mud, centrifuging, and inoculating the supernatant into a culture medium for culture to obtain a water body bacterial liquid;
taking water body bacteria liquid for gradient dilution, then coating the water body bacteria liquid on a solid culture medium for culture, separating to obtain a single colony, and identifying and screening the single colony;
respectively carrying out amplification culture on bacillus subtilis, bacillus amyloliquefaciens, lactobacillus acidophilus, bacillus coagulans, bacillus licheniformis and pseudomonas aeruginosa, and then mixing the strains to obtain the composite microbial preparation.
7. The microbial composite water purifying agent of claim 6, wherein the total viable bacteria concentration of the obtained composite microbial preparation is 8-20 x 108CFU/g。
8. The microbial composite water purifying agent of claim 1, wherein the preparation method of the water purifying agent composite carrier comprises the following steps:
mixing polyacrylamide, polyaluminum ferric chloride, polyether quaternary ammonium salt, sulfonated coal, zeolite, coconut shell carbon, chitosan and a certain amount of water, heating, stirring, adding diatom ooze, clay and hydroxyapatite, stirring, and filtering to obtain a solid substance;
and drying the solid matter, and granulating to obtain the water purifying agent composite carrier.
9. The microbial composite water purifying agent of claim 8, wherein the particle size of the obtained water purifying agent composite carrier is 1-6 mm.
10. The method for preparing the microbial composite water purifying agent of any one of claims 1 to 9, which is characterized by comprising the following steps:
centrifuging the compound microbial preparation, and removing supernatant to obtain wet thalli;
taking wet bacteria, carrying out heavy suspension by using a certain amount of culture medium, adding the water purifying agent composite carrier, and culturing to obtain the microbial composite water purifying agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911328007.5A CN110980967A (en) | 2019-12-20 | 2019-12-20 | Microbial composite water purifying agent and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911328007.5A CN110980967A (en) | 2019-12-20 | 2019-12-20 | Microbial composite water purifying agent and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110980967A true CN110980967A (en) | 2020-04-10 |
Family
ID=70074331
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911328007.5A Pending CN110980967A (en) | 2019-12-20 | 2019-12-20 | Microbial composite water purifying agent and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110980967A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113264633A (en) * | 2021-04-20 | 2021-08-17 | 广西壮族自治区环境保护科学研究院 | Ecological restoration method for black and odorous river channel |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101629157A (en) * | 2009-08-06 | 2010-01-20 | 江苏省江大绿康生物工程技术研究有限公司 | Compound microecologics for purifying water quality |
| CN103497906A (en) * | 2013-08-02 | 2014-01-08 | 国家***第三海洋研究所 | Microecological preparation, preparation method, and applications thereof |
| CN103923906A (en) * | 2013-09-25 | 2014-07-16 | 领绿生物镇江有限公司 | Microbial preparation with water purification and bottom modification dual effects and its preparation method |
| CN106587381A (en) * | 2016-12-20 | 2017-04-26 | 大连赛姆生物工程技术有限公司 | Composite microorganism water purifying agent for sea cucumber aquaculture water |
| CN108624531A (en) * | 2018-05-15 | 2018-10-09 | 北京师范大学 | A kind of complex microorganism water purification agent and its application |
| CN110331105A (en) * | 2019-07-11 | 2019-10-15 | 湖北真福医药有限公司 | A kind of sewage treatment composite bacteria agent and preparation method thereof |
| CN110438046A (en) * | 2019-08-21 | 2019-11-12 | 江西清瀞自然环境科技有限公司 | Compound micro-ecological preparation and preparation method thereof for the black smelly water harnessing of river swag |
-
2019
- 2019-12-20 CN CN201911328007.5A patent/CN110980967A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101629157A (en) * | 2009-08-06 | 2010-01-20 | 江苏省江大绿康生物工程技术研究有限公司 | Compound microecologics for purifying water quality |
| CN103497906A (en) * | 2013-08-02 | 2014-01-08 | 国家***第三海洋研究所 | Microecological preparation, preparation method, and applications thereof |
| CN103923906A (en) * | 2013-09-25 | 2014-07-16 | 领绿生物镇江有限公司 | Microbial preparation with water purification and bottom modification dual effects and its preparation method |
| CN106587381A (en) * | 2016-12-20 | 2017-04-26 | 大连赛姆生物工程技术有限公司 | Composite microorganism water purifying agent for sea cucumber aquaculture water |
| CN108624531A (en) * | 2018-05-15 | 2018-10-09 | 北京师范大学 | A kind of complex microorganism water purification agent and its application |
| CN110331105A (en) * | 2019-07-11 | 2019-10-15 | 湖北真福医药有限公司 | A kind of sewage treatment composite bacteria agent and preparation method thereof |
| CN110438046A (en) * | 2019-08-21 | 2019-11-12 | 江西清瀞自然环境科技有限公司 | Compound micro-ecological preparation and preparation method thereof for the black smelly water harnessing of river swag |
Non-Patent Citations (1)
| Title |
|---|
| 孙福新，宋娴丽主编: "《规模化海水养殖园区环境工程生态优化技术体系》", 30 September 2017, 中国海洋大学出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113264633A (en) * | 2021-04-20 | 2021-08-17 | 广西壮族自治区环境保护科学研究院 | Ecological restoration method for black and odorous river channel |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104894099B (en) | A kind of purification of water quality bacteria adhension particle and preparation method thereof | |
| CN112657465B (en) | Preparation method of magnetic biochar and method for treating tailing wastewater | |
| CN102965365B (en) | Preparation method for microbial nanospheres for water quality purification | |
| CN105347513B (en) | A kind of preparation method and applications of probiotics | |
| CN107434305B (en) | Water purifying agent with defect-rich carbon carrier immobilized microorganisms and preparation method thereof | |
| WO2012096481A2 (en) | Microbial preparation comprising mixed microorganisms, and a biological treatment method for rivers and lakes and a sludge autodigestion process using the same | |
| CN105582890A (en) | Preparation method and application of composite nano biomass charcoal material | |
| CN107473404B (en) | Water purifying agent with self-formed block-shaped carbon carrier for fixing microorganisms and preparation method thereof | |
| CN109706096B (en) | Cold-resistant brevibacterium strain with denitrification and efficient flocculation capacity and application thereof | |
| CN105921099B (en) | Biochemical preparation for administering Threonine Fermentation sewage | |
| CN113699057B (en) | Rhodococcus toonapus with heterotrophic nitrification-aerobic denitrification function and application thereof | |
| CN108585179B (en) | Compound microbial preparation for water pollution treatment and preparation method thereof | |
| Ye et al. | An effective biological treatment method for marine aquaculture wastewater: Combined treatment of immobilized degradation bacteria modified by chitosan-based aerogel and macroalgae (Caulerpa lentillifera) | |
| CN110980967A (en) | Microbial composite water purifying agent and preparation method thereof | |
| CN108993425A (en) | A kind of compound biological adsorption agent and its application | |
| CN110964713A (en) | Preparation method of immobilized microorganism particles for removing ammonia nitrogen from black and odorous water | |
| CN111944799A (en) | Preparation method and application of immobilized particles embedded with thiobacillus denitrificans | |
| Shan et al. | Ammonia and nitrite nitrogen removal in shrimp culture by Vibrio alginolyticus VZ5 immobilized in SA beads | |
| CN113321318B (en) | Method for improving efficiency of clay in treating harmful algal blooms based on microbial composite modification | |
| CN111378592A (en) | Bacillus licheniformis and method for treating malodorous organic wastewater by using same to purify water | |
| CN112694997B (en) | Composite microorganism bacterial stock solution suitable for sewage treatment and active liquid culture method | |
| CN106045201B (en) | The biological treatment of xanthan gum fermentation waste | |
| CN108546662B (en) | Method for jointly treating aquaculture wastewater by respectively immobilized nitrifying bacteria group-bacillus | |
| CN107512780B (en) | Bacterial carbon water purifying agent for removing high-concentration ammonia nitrogen in aquaculture sewage and preparation method thereof | |
| CN111484939A (en) | Separation and application of Microbeiella macroscopia |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200410 |
|
| RJ01 | Rejection of invention patent application after publication |