CN110975001B - Chitosan-cellulose composite hemostatic sponge and preparation method and application thereof - Google Patents

Chitosan-cellulose composite hemostatic sponge and preparation method and application thereof Download PDF

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CN110975001B
CN110975001B CN201911254212.1A CN201911254212A CN110975001B CN 110975001 B CN110975001 B CN 110975001B CN 201911254212 A CN201911254212 A CN 201911254212A CN 110975001 B CN110975001 B CN 110975001B
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chitosan
cellulose
dac
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hemostatic sponge
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CN110975001A (en
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林松
魏晓慧
田丰
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Institute of Medical Support Technology of Academy of System Engineering of Academy of Military Science
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
    • A61L24/0047—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
    • A61L24/0052—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material with an inorganic matrix
    • A61L24/0068—Inorganic materials not covered by groups A61L24/0057 or A61L24/0063
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
    • A61L24/001—Use of materials characterised by their function or physical properties
    • A61L24/0036—Porous materials, e.g. foams or sponges
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/418—Agents promoting blood coagulation, blood-clotting agents, embolising agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2400/00—Materials characterised by their function or physical properties
    • A61L2400/04—Materials for stopping bleeding

Abstract

The invention belongs to the technical field of biomedical materials, and discloses a chitosan-cellulose composite hemostatic sponge, and a preparation method and application thereof. The preparation method comprises the following steps: (1) using NaIO4Oxidizing the cellulose nanocrystal to prepare dialdehyde cellulose; (2) the chitosan with the molecular weight of 180000-300000Da and the deacetylation degree of 80-95 percent and the dialdehyde cellulose are sold in the mass ratio of mChitosan∶mCellulose dialdehyde0.2-10 to prepare the compound hemostatic sponge. The composite hemostatic sponge has the advantages of good blood coagulation effect and strong absorption capacity.

Description

Chitosan-cellulose composite hemostatic sponge and preparation method and application thereof
Technical Field
The invention relates to the technical field of biomedical materials, in particular to a chitosan-cellulose composite hemostatic sponge, a preparation method and application thereof.
Background
The description of the background of the invention pertaining to the related art to which this invention pertains is given for the purpose of illustration and understanding only of the summary of the invention and is not to be construed as an admission that the applicant is explicitly or implicitly admitted to be prior art to the date of filing this application as first filed with this invention.
Traumatic hemorrhage is a major cause of military and civilian casualties. It has been reported that aortic hemorrhage causes 50% of battlefield casualties and 31% of mortality in civilian clinical settings. Timely hemostasis is vital to reducing blood loss, treating bleeding wounds and improving survival rate of treatment.
Chitosan (CS) is a linear polysaccharide, the structural formula of which is beta- (1 → 4) -2-amino-2-deoxy-D-glucose, which can be obtained from Chitin (Chitin), one of the most abundant natural polymers in the world, through a deacetylation process, and is a green natural polymer with wide sources and low price, and the Chitosan (CS) has wide application in the aspects of wound dressing, drug delivery, genetic engineering, tissue engineering and the like so far. Chitosan has excellent performances in the aspects of hemostasis, antibacterial activity, cell and tissue compatibility, toxicity, biodegradability and the like, and is one of representative hemostatic materials. The main hemostatic mechanism of chitosan is that it has a certain amount of positive charges, and can promote the aggregation of red blood cells and increase the adhesion of platelets, thereby achieving the purpose of hemostasis and promoting the healing of wounds.
The cellulose nanocrystal is a natural material which is extracted from natural cellulose and has plant source and application prospect, consists of D-anhydroglucose units connected by beta-1-4, and has huge yield and low cost. Due to its excellent mechanical strength, special surface chemical properties and excellent biological properties are receiving great attention as biomedical materials.
The existing hemostatic sponge has the problems of poor blood coagulation effect and poor liquid absorption capacity.
Disclosure of Invention
The invention aims to provide a chitosan-cellulose composite hemostatic sponge, a preparation method and application thereof. The composite hemostatic sponge has the advantages of good blood coagulation effect and strong imbibition capability.
A preparation method of chitosan-cellulose composite hemostatic sponge comprises the following steps:
(1) using NaIO4Oxidizing the cellulose nanocrystal to prepare dialdehyde cellulose;
(2) the chitosan with the molecular weight of 180000-300000Da and the deacetylation degree of 80-95 percent and the dialdehyde cellulose are sold in the mass ratio of mChitosan：mCellulose dialdehyde0.2-10 to prepare the compound hemostatic sponge.
Further, the step (1) specifically comprises the following steps:
taking sodium metaperiodate NaIO4Adding the mixture into deionized water with the concentration of 2.0 wt% -5.0 wt%, and adding cellulose nanocrystalline after complete dissolution, wherein m isNaIO4：mCellulose nanocrystalsStirring at room temperature for 24-72 h under dark conditions of 0.8-1.6, adding 10mL of ethylene glycol, reacting for 0.5-1h, and removing excessive NaIO4To obtain NaIO4An oxidized cellulose nanocrystal dispersion; subjecting the NaIO to4Centrifuging the oxidized cellulose nanocrystal dispersion to obtain a gel at the bottom, and sequentially adding deionized water and the volume percentWashing with 50% ethanol solution, anhydrous ethanol and deionized water, freezing at-20 deg.C, and freeze drying in freeze dryer to obtain dialdehyde cellulose.
Further, the step (2) specifically comprises the following steps:
preparing the dialdehyde cellulose into an aqueous solution with the concentration of 0.2 to 20 weight percent, and cooling the aqueous solution for later use; dissolving the chitosan powder in 1% (v/v) acetic acid solution to prepare 0.5-5 wt% chitosan solution according to mass ratio of mChitosan：mCellulose dialdehydeMixing dialdehyde cellulose solution and chitosan solution 0.2-10, stirring at room temperature for 16-24 h, adding sodium cyanoborohydride 5-10% of chitosan, stirring for 2-4h, dialyzing in dialysis bag until pH value is 6.6-7.2, and freeze drying.
In a second aspect, the invention provides a chitosan-cellulose composite hemostatic sponge, which is prepared by the preparation method.
In a third aspect, the invention provides an application of chitosan-cellulose composite hemostatic sponge in hemostatic dressing, and the chitosan-cellulose composite hemostatic sponge is prepared by the preparation method.
The embodiment of the invention has the following beneficial effects:
the method of the invention has simple operation and mild reaction conditions in the whole process, and the prepared composite sponge has a porous structure and good mechanical property, coagulation property and liquid absorption capacity.
The obtained hemostatic sponge is prepared into a three-dimensional porous structure by a vacuum freeze drying method, has good liquid absorption, is beneficial to red blood cell aggregation, and accelerates the promotion of blood coagulation.
The chitosan with high deacetylation degree and the cellulose are combined in a chemical covalent mode, so that the excellent performance of the chitosan as a hemostatic material is kept, the advantages of good mechanical property and good hydrophilicity of dialdehyde cellulose are added, the two materials play a role in cooperation, and the application of the obtained composite sponge in the aspect of hemostatic dressing is very important.
The reaction conditions in the embodiment of the application are mild, the reaction is carried out in aqueous solution at room temperature, and only one step uses weak acid as a solvent medium;
the porous structure improves the liquid absorption capacity and the air permeability, and is more favorable for being used as a hemostatic dressing;
compared with the existing mode of physically combining the two products by electrostatic adsorption, the Schiff base cross-linking chemical reaction enables the two products to be combined in a conjugate way, the stable structure is more favorable for exerting the synergistic blood coagulation performance, and the hemostatic effect is obviously improved compared with that of a single chitosan or cellulose product.
Drawings
FIG. 1 is a scanning electron microscope image of a composite sponge in the preparation method of chitosan-cellulose composite hemostatic sponge of the present invention.
Fig. 2 is a schematic diagram illustrating TEG parameters of a thromboelastometer in the preparation method of the chitosan-cellulose composite hemostatic sponge.
FIG. 3 is the weight gain ratio of the composite sponge material after water absorption in the preparation method of the chitosan-cellulose composite hemostatic sponge of the invention.
Detailed Description
The present application is further described below with reference to examples.
In the following description, different "one embodiment" or "an embodiment" may not necessarily refer to the same embodiment, in order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art. Various embodiments may be replaced or combined, and other embodiments may be obtained according to the embodiments without creative efforts for those skilled in the art.
The invention takes cellulose and chitosan as base materials, adopts a chemical method, and firstly utilizes NaIO4The cellulose is selectively oxidized to obtain dialdehyde cellulose subjected to ring opening oxidation at 2, 3 positions, Schiffbase crosslinking reaction is carried out on the aldehyde group and the chitosan to enhance the combination effect of the dialdehyde cellulose and the chitosan, and meanwhile, the hemostatic sponge obtained by a vacuum freeze drying mode is of a three-dimensional porous structure, so that the hydrophilicity of the chitosan is improved, the moisture can be rapidly absorbed, the erythrocyte is concentrated, and the erythrocyte aggregation is promoted, thereby accelerating the speed ofBlood coagulation, the hemostasis effect of reinforcing can promote the mechanical strength of sponge to a certain extent again, effectively overcome chitosan powder self intensity difference, the shaping is difficult, does not change shortcomings such as use.
A preparation method of chitosan-cellulose composite hemostatic sponge comprises the following steps:
(1) using NaIO4Oxidizing the cellulose nanocrystal to prepare dialdehyde cellulose;
(2) the chitosan and the dialdehyde cellulose are mixed according to the mass ratio of mChitosan：mCellulose dialdehyde0.2-10 to prepare the compound hemostatic sponge.
In some embodiments of the present invention, the step (1) specifically includes the following steps:
taking sodium metaperiodate NaIO4Adding the mixture into deionized water with the concentration of 2.0-5.0 wt%, and adding cellulose nanocrystalline after complete dissolution, wherein m isNaIo4：mCellulose nanoparticiesStirring under dark condition for 24-72 h (0.8-1.6), adding NaIO to remove excessive NaIO4To obtain NaIO4An oxidized cellulose nanocrystal dispersion; subjecting the NaIO to4Centrifuging the oxidized cellulose nanocrystal dispersion liquid to obtain gel at the bottom, sequentially washing with deionized water, 50% ethanol solution by volume, absolute ethanol and deionized water, freezing at-20 ℃, and freeze-drying in a freeze dryer to obtain the dialdehyde cellulose.
In some embodiments of the present invention, the step (2) specifically includes the following steps:
preparing the dialdehyde cellulose into an aqueous solution with the concentration of 0.2 to 20 weight percent, and cooling the aqueous solution for later use; dissolving the deacetylated chitosan powder with the molecular weight of 180000-300000Da and the deacetylation degree of 80-95% in 1% (v/v) acetic acid solution to prepare a chitosan solution with the concentration of 0.5-5 wt%, wherein the chitosan solution is m in mass ratioChitosan：mCellulose dialdehydeMixing dialdehyde cellulose solution and chitosan solution 0.2-10, stirring at room temperature for 16-24 h, adding sodium cyanoborohydride 5-10 wt% of chitosan, stirring for 2-4h, and placing in dialysis bagDialyzing to pH 6.6-7.2, and freeze drying.
The chitosan-cellulose composite hemostatic sponge is prepared by the preparation method, and the obtained hemostatic sponge has a porous structure (figure 1), has good liquid absorption, is favorable for erythrocyte aggregation and promotes blood coagulation.
The reaction conditions in the embodiment of the application are mild, the reaction is carried out in aqueous solution at room temperature, and only one step uses weak acid as a solvent medium;
the porous structure improves the liquid absorption capacity and the air permeability, and is more favorable for being used as a hemostatic dressing;
compared with the existing mode of combining the two by electrostatic adsorption, the Schiff base crosslinking reaction enables the two to be combined in a conjugate way, the stable structure is more favorable for exerting the synergistic blood coagulation performance, and the hemostatic effect is obviously improved compared with that of a single chitosan or cellulose product.
Example 1
A preparation method of chitosan-cellulose composite hemostatic sponge comprises the following steps:
(1) dialdehyde cellulose: taking a certain amount of sodium metaperiodate NaIO4Adding the mixture into 100mL of deionized water, and adding the cellulose nanocrystalline CNC m with a corresponding proportion after the mixture is completely dissolvedNaIo4：mCNCStirring under dark conditions for 24h (1.6), adding 10mL of ethylene glycol, reacting for 0.5-1h, and removing excessive NaIO4To obtain NaIO4An oxidized cellulose nanocrystal dispersion;
(2) centrifuging the solution obtained in the step (1), obtaining gel at the bottom, sequentially washing with deionized water, 50% ethanol solution, absolute ethanol and deionized water, freezing at-20 ℃, and freeze-drying in a freeze dryer to obtain dialdehyde cellulose (DAC);
(3) adding a certain amount of DAC into water, heating and dissolving under the condition of oil bath at 100 ℃ to prepare a DAC aqueous solution, and cooling for later use;
(4) compounding the sponge: dissolving a certain amount of chitosan powder with a molecular weight of 180000Da and a deacetylation degree of 85% in 1% (v/v) acetic acid solution to obtain a concentration of 5%Adding a DAC aqueous solution into a wt% chitosan solution according to a corresponding proportion, wherein the mass ratio of the DAC aqueous solution is mCS：mDAC1. Stirring at room temperature for 16h, adding a small amount of sodium cyanoborohydride, stirring for 2-4h, dialyzing the obtained solution in a dialysis bag for 2-4 days until the pH value is close to neutral, pouring into a pore plate or a plate, freezing, and freeze-drying.
Example 2
A preparation method of chitosan-cellulose composite hemostatic sponge comprises the following steps:
(1) dialdehyde cellulose: taking a certain amount of sodium metaperiodate NaIO4Adding the mixture into 100mL of deionized water, and adding the cellulose nanocrystalline CNC m with a corresponding proportion after the mixture is completely dissolvedNaIO4：mCNCStirring under dark condition for 48h while changing to 1, adding 10mL of ethylene glycol, reacting for 0.5-1h, removing excessive NaIO4To obtain NaIO4An oxidized cellulose nanocrystal dispersion;
(2) centrifuging the solution obtained in the step (1), obtaining gel at the bottom, sequentially washing with deionized water, 50% ethanol solution, absolute ethanol and deionized water, freezing at-20 ℃, and freeze-drying in a freeze dryer to obtain dialdehyde cellulose (DAC);
(3) adding a certain amount of DAC into water, heating and dissolving under the condition of oil bath at 100 ℃ to prepare a DAC aqueous solution, and cooling for later use;
(4) compounding the sponge: dissolving a certain amount of chitosan powder with molecular weight of 270000Da and deacetylation degree of 90% in 1% (v/v) acetic acid solution to prepare chitosan solution with concentration of 1 wt%, adding DAC water solution according to corresponding proportion, wherein the mass ratio of DAC water solution is mCS：m DAC4. Stirring at room temperature for 20h, adding a small amount of sodium cyanoborohydride, stirring for 2-4h, dialyzing the obtained solution in a dialysis bag for 2-4 days until the pH value is close to neutral, pouring into a pore plate or a plate, freezing, and freeze-drying.
Example 3
A preparation method of chitosan-cellulose composite hemostatic sponge comprises the following steps:
(1) dialdehyde cellulose: taking a certain amount of sodium metaperiodate NaIO4Adding the mixture into 100mL of deionized water, and adding the cellulose nanocrystalline CNC m with a corresponding proportion after the mixture is completely dissolvedNaIo4：mCNCStirring under dark condition for 48h (1.3), adding 10mL of ethylene glycol, reacting for 0.5-1h, and removing excessive NaIO4To obtain NaIO4An oxidized cellulose nanocrystal dispersion;
(2) centrifuging the solution obtained in the step (1), obtaining gel at the bottom, sequentially washing with deionized water, 50% ethanol solution, absolute ethanol and deionized water, freezing at-20 ℃, and freeze-drying in a freeze dryer to obtain dialdehyde cellulose (DAC);
(3) adding a certain amount of DAC into water, heating and dissolving under the condition of oil bath at 100 ℃ to prepare a DAC aqueous solution, and cooling for later use;
(4) compounding the sponge: dissolving a certain amount of chitosan powder with the molecular weight of 180000Da and the deacetylation degree of 85% in 1% (v/v) acetic acid solution to prepare chitosan solution with the concentration of 2 wt%, adding DAC (digital-to-analog converter) aqueous solution according to the corresponding proportion, wherein the mass ratio of the DAC aqueous solution is mCS：mDAC6. Stirring at room temperature for 24h, adding a small amount of sodium cyanoborohydride, stirring for 2-4h, dialyzing the obtained solution in a dialysis bag for 2-4 days until the pH value is 6.6-7.2, pouring into a pore plate or a flat dish, freezing, and freeze-drying.
Example 4
A preparation method of chitosan-cellulose composite hemostatic sponge comprises the following steps:
(1) dialdehyde cellulose: taking a certain amount of sodium metaperiodate NaIO4Adding the mixture into 100mL of deionized water, and adding the cellulose nanocrystalline CNC m with a corresponding proportion after the mixture is completely dissolvedNaIO4：mCNCStirring under dark conditions for 72h (1.3), adding 10mL of ethylene glycol, reacting for 0.5-1h, and removing excessive NaIO4To obtain NaIO4An oxidized cellulose nanocrystal dispersion;
(2) centrifuging the solution obtained in the step (1), obtaining gel at the bottom, sequentially washing with deionized water, 50% ethanol solution, absolute ethanol and deionized water, freezing at-20 ℃, and freeze-drying in a freeze dryer to obtain dialdehyde cellulose (DAC);
(3) adding a certain amount of DAC into water, heating and dissolving under the condition of oil bath at 100 ℃ to prepare a DAC aqueous solution, and cooling for later use;
(4) compounding the sponge: dissolving a certain amount of chitosan powder with the molecular weight of 300000Da and the deacetylation degree of 95% in 1% (v/v) acetic acid solution to prepare a chitosan solution with the concentration of 1 wt%, and adding a DAC (digital-to-analog converter) aqueous solution according to the corresponding proportion, wherein the mass ratio of the DAC aqueous solution is mCS：m DAC2. Stirring at room temperature for 24h, adding a small amount of sodium cyanoborohydride, stirring for 2-4h, dialyzing the obtained solution in a dialysis bag for 2-4 days until the pH value is 6.6-7.2, pouring into a pore plate or a flat dish, freezing, and freeze-drying.
Example 5
A preparation method of chitosan-cellulose composite hemostatic sponge comprises the following steps:
(1) dialdehyde cellulose: taking a certain amount of sodium metaperiodate NaIO4Adding the mixture into 100mL of deionized water, and adding the cellulose nanocrystalline CNC m with a corresponding proportion after the mixture is completely dissolvedNaIO4：mCNCStirring under dark conditions for 72h (1.6), adding 10mL of ethylene glycol, reacting for 0.5-1h, and removing excessive NaIO4To obtain NaIO4An oxidized cellulose nanocrystal dispersion;
(2) centrifuging the solution obtained in the step (1), obtaining gel at the bottom, sequentially washing with deionized water, 50% ethanol solution, absolute ethanol and deionized water, freezing at-20 ℃, and freeze-drying in a freeze dryer to obtain dialdehyde cellulose (DAC);
(3) adding a certain amount of DAC into water, heating and dissolving under the condition of oil bath at 100 ℃ to prepare a DAC aqueous solution, and cooling for later use;
(4) compounding the sponge: dissolving a certain amount of chitosan powder with the molecular weight of 300000Da and the deacetylation degree of 85% in 1% (v/v) acetic acid solution to prepare a chitosan solution with the concentration of 0.5 wt%, and adding a DAC (digital-to-analog converter) aqueous solution according to the corresponding proportion, wherein the mass ratio of the DAC aqueous solution is mCS：m DAC2. Stirring at room temperature for 20h, and adding a small amount of sodium cyanoborohydride (NaBH)3CN), stirring for 2-4h, dialyzing the obtained solution in a dialysis bag for 2-4 days until the pH value is up to6.6-7.2, pouring into a pore plate or a flat dish, freezing and freeze-drying.
Blood coagulation performance test
The blood coagulation effect of the obtained composite sponge is evaluated by a Thromboelastography (TEG), and the significance of indexes obtained by testing is shown in figure 2 (R: time for forming the first obvious thrombus, K: time for the thrombus to reach specific hardness, A: dynamics of thrombus formation, MA: maximum strength of thrombus formation), and the specific testing method is as follows:
taking a certain amount of lyophilized sponge, pulverizing to 60 meshes, sieving, adding 1mL of anticoagulated whole blood, mixing uniformly by vortex, taking 340 microliter of mixed solution, adding into a test cup, and adding 20 microliter of 0.2mol/L CaCl2Solution, test, repeat two sets. And (3) comparing the blood coagulation effect of the CS and the CNC freeze-dried sponge. The TEG data for the different examples are shown in table 1.
TABLE 1
Figure BDA0002309546740000091
In conclusion, the cellulose nanocrystal modified chitosan composite sponge base material prepared by the method provided by the invention has a three-dimensional porous appearance, and compared with a chitosan base material used alone, the blood coagulation effect is remarkably improved, the R value of the thrombus formation time and the K value of the time reaching the specific hardness are both remarkably reduced through an optimized formula, and the maximum strength of the thrombus formation is remarkably improved. Meanwhile, the prepared hemostatic sponge has the capability of efficiently and quickly absorbing water, can quickly absorb water within 10s after being placed in PBS (phosphate buffer solution), can reach the maximum self weight of more than fifteen times (figure 3), and is favorable for gathering red blood cells, thereby accelerating the blood coagulation effect.
It should be noted that the above embodiments can be freely combined as necessary. The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (3)

1. A preparation method of chitosan-cellulose composite hemostatic sponge is characterized by comprising the following steps:
(1) dialdehyde cellulose: taking a certain amount of sodium metaperiodate NaIO4Adding the mixture into 100mL of deionized water, and adding the cellulose nanocrystalline CNC m with a corresponding proportion after the mixture is completely dissolvedNaIO4:mCNCStirring under dark conditions for 72h (1.3), adding 10mL of ethylene glycol, reacting for 0.5-1h, and removing excessive NaIO4To obtain NaIO4An oxidized cellulose nanocrystal dispersion;
(2) centrifuging the solution obtained in the step (1), obtaining gel at the bottom, sequentially cleaning with deionized water, 50% ethanol solution, absolute ethanol and deionized water, freezing at-20 ℃, and freeze-drying in a freeze dryer to obtain dialdehyde cellulose;
(3) adding a certain amount of DAC into water, heating and dissolving under the condition of oil bath at 100 ℃ to prepare a DAC aqueous solution, and cooling for later use;
(4) compounding the sponge: dissolving a certain amount of chitosan powder with the molecular weight of 300000Da and the deacetylation degree of 95% in 1% (v/v) acetic acid solution to prepare a chitosan solution with the concentration of 1 wt%, and adding a DAC (digital-to-analog converter) aqueous solution according to the corresponding proportion, wherein the mass ratio of the DAC aqueous solution is mCS：mDAC2; stirring at room temperature for 24h, adding a small amount of sodium cyanoborohydride, stirring for 2-4h, dialyzing the obtained solution in a dialysis bag for 2-4 days until the pH value is 6.6-7.2, pouring into a pore plate or a flat dish, freezing, and freeze-drying.
2. A chitosan-cellulose composite hemostatic sponge, wherein the chitosan-cellulose composite hemostatic sponge is prepared by the preparation method of claim 1.
3. The application of chitosan-cellulose composite hemostatic sponge in preparing hemostatic dressings is characterized in that the chitosan-cellulose composite hemostatic sponge is prepared by the preparation method of claim 1.
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CN113244442B (en) * 2021-06-01 2022-11-15 北京化工大学 Application of polyamino cationic compound in preparation of surface procoagulant enhanced hemostatic material
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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106178066A (en) * 2015-05-07 2016-12-07 中国科学院化学研究所 A kind of modified cellulose/chitosan compound hemostatic material and preparation method and application
CN107847635A (en) * 2015-05-06 2018-03-27 捷锐士阿希迈公司(以奥林巴斯美国外科技术名义) Carboxymerhyl chitosan sponge preparation
CN109364287A (en) * 2018-10-08 2019-02-22 中国海洋大学 A kind of polysaccharide bleeding-stopping dressing and its preparation method and application

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040101546A1 (en) * 2002-11-26 2004-05-27 Gorman Anne Jessica Hemostatic wound dressing containing aldehyde-modified polysaccharide and hemostatic agents

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107847635A (en) * 2015-05-06 2018-03-27 捷锐士阿希迈公司(以奥林巴斯美国外科技术名义) Carboxymerhyl chitosan sponge preparation
CN106178066A (en) * 2015-05-07 2016-12-07 中国科学院化学研究所 A kind of modified cellulose/chitosan compound hemostatic material and preparation method and application
CN109364287A (en) * 2018-10-08 2019-02-22 中国海洋大学 A kind of polysaccharide bleeding-stopping dressing and its preparation method and application

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
刘文迎.氧化纤维素/壳聚糖复合止血材料的制备及性能研究.《中国优秀硕士学位论文全文数据库，工程科技I辑》.2019, *
氧化纤维素/壳聚糖复合止血材料的制备及性能研究;刘文迎;《中国优秀硕士学位论文全文数据库，工程科技I辑》;20190115;第15、18、23、25页 *

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