CN110887885A - Dissolved oxygen electrochemical sensor for micro-fluidic chip and preparation method - Google Patents
Dissolved oxygen electrochemical sensor for micro-fluidic chip and preparation method Download PDFInfo
- Publication number
- CN110887885A CN110887885A CN201911187616.3A CN201911187616A CN110887885A CN 110887885 A CN110887885 A CN 110887885A CN 201911187616 A CN201911187616 A CN 201911187616A CN 110887885 A CN110887885 A CN 110887885A
- Authority
- CN
- China
- Prior art keywords
- dissolved oxygen
- solution
- electrochemical sensor
- electrode
- oxygen electrochemical
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/403—Cells and electrode assemblies
- G01N27/406—Cells and probes with solid electrolytes
- G01N27/407—Cells and probes with solid electrolytes for investigating or analysing gases
- G01N27/409—Oxygen concentration cells
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
Abstract
A dissolved oxygen electrochemical sensor for a microfluidic chip is prepared by using a conductive substrate, a breathable film layer and an electrolyte layer, and realizes detection of dissolved oxygen in an electrolyte solution. The conductive substrate can be an electrode made of common materials such as a screen printing electrode, a magnetron sputtering electrode and the like, and the dissolved oxygen electrochemical sensor can be cut into any size, shape and thickness according to the design of a micro-fluidic chip needing to be matched; the dissolved oxygen electrochemical sensor is an all-solid-state electrode after being prepared, the storage is convenient, the preparation process of the electrode is simple, and the operability is strong; after the solution of the breathable film layer and the solution of the electrolyte film layer of the dissolved oxygen electrochemical sensor are prepared, the solution can be stored at room temperature for a long time, so that the cost is saved; the dissolved oxygen electrochemical sensor prepared by using the conductive substrates made of different insulating materials and conductive materials has good linear response when the concentration of dissolved oxygen is within the range of 1-20 mg/L.
Description
Technical Field
The invention relates to a dissolved oxygen electrochemical sensor for a microfluidic chip and a preparation method thereof, belonging to the technical field of microfluidics.
Background
Molecular oxygen dissolved in water is called dissolved oxygen. The blood serum of human body contains dissolved oxygen, which is sucked into the blood from lung through capillary and then transferred to organs or cells of various parts of body for use. Many clinical diseases cause human hypoxia, which directly affects the normal metabolism of human cells, further harms human organs, and seriously threatens human life. Real-time monitoring of arterial blood oxygen concentration is very important in clinical care.
The generation and development of the microfluidic technology open up a new way for the detection of dissolved oxygen, and the microfluidic chip integrates basic operation units such as sample preparation, reaction, separation, detection and the like in the processes of biological, chemical and medical analysis on a micron-scale chip to automatically complete the whole analysis process, is simple and convenient to operate, has quick response time and is suitable for joint inspection of a plurality of projects. At the present stage, research and development personnel are always dedicated to seeking a simple, economic and rapid dissolved oxygen detection technical scheme for a microfluidic chip for improving the detection efficiency and accuracy of dissolved oxygen in blood and reducing the monitoring cost.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides the dissolved oxygen electrochemical sensor for the microfluidic chip and the preparation method thereof, so that the high-efficiency and accurate detection of the dissolved oxygen in the blood is realized, and the detection cost of the dissolved oxygen is reduced.
The technical scheme for solving the technical problems is as follows: the dissolved oxygen electrochemical sensor for the micro-fluidic chip comprises a conductive substrate formed by an insulating material and a conductive material, wherein the conductive material covers the surface of the insulating material, an electrolyte layer covers the surface of the conductive material, and a breathable film layer covers the surface of the electrolyte layer.
As a preferable scheme of the dissolved oxygen electrochemical sensor for the microfluidic chip, the insulating material of the conductive substrate is a silicon plate, PET or PMMA; the conductive material of the conductive substrate is gold, platinum, carbon or silver.
As a preferable scheme of the dissolved oxygen electrochemical sensor for the microfluidic chip, the electrolyte layer is gelatin, the gelatin is skin gelatin or bone gelatin, and the gelatin strength of the gelatin is 100-300 g Bloom.
As a preferable scheme of the dissolved oxygen electrochemical sensor for the microfluidic chip, the air-permeable membrane layer is made of a material with permeability to gas, and the air-permeable membrane layer is made of one of polyvinyl chloride with a K-value of 55-72, organic siloxane and cellulose acetate with an acetyl content of 37% -42%.
As a preferable scheme of the dissolved oxygen electrochemical sensor for the microfluidic chip, the conductive substrate adopts one of a screen printing electrode, a magnetron sputtering electrode, a spraying electrode, an evaporation electrode or a chemical plating electrode.
The invention also provides a preparation method of the dissolved oxygen electrochemical sensor for the microfluidic chip, which comprises the following steps:
preparing electrolyte layer solution in advance, weighing quantitative gelatin, adding the gelatin into the prepared electrolyte aqueous solution under the condition of magnetic stirring, and stirring for 0.5-1 hour by magnetic force to completely dissolve the gelatin in the electrolyte aqueous solution to prepare 10-20% electrolyte layer solution;
uniformly covering the electrolyte layer solution on a conductive substrate, and drying in an oven at 37 ℃ for more than 3 hours;
preparing a breathable film layer solution in advance, weighing a certain amount of breathable film material, adding the breathable film material into an organic solvent under the condition of magnetic stirring, and stirring for 0.5-2 hours by magnetic force to completely dissolve the breathable film material in the organic solvent to prepare a 5-20% breathable film layer solution;
and uniformly covering the surface of the electrolyte layer with the solution of the breathable film layer, and airing in a fume hood at the temperature of 20-25 ℃ for 0.5-4 hours to prepare the dissolved oxygen electrochemical sensor suitable for the microfluidic chip.
As a preferred scheme of the preparation method of the dissolved oxygen electrochemical sensor for the microfluidic chip, the organic solvent of the breathable film layer solution is cyclohexanone, propiophenone, chloroform or acetone.
As a preferable scheme of the preparation method of the dissolved oxygen electrochemical sensor for the microfluidic chip, 0.1mol/L, 0.5mol/L or 1mol/L KCl solution is adopted as the electrolyte aqueous solution, or 0.1mol/L, 0.5m KCl solution is adopted as the electrolyte aqueous solutionol/L or 1mol/L KNO3And (3) solution.
As a preferable scheme of the preparation method of the dissolved oxygen electrochemical sensor for the microfluidic chip, the method of uniformly covering the electrolyte layer solution on the conductive substrate is one of a dropping coating method, a spin coating method, a machine spotting method or a machine slip film method;
as a preferable embodiment of the method for manufacturing the dissolved oxygen electrochemical sensor for the microfluidic chip, the method for uniformly covering the surface of the electrolyte layer with the solution of the gas permeable membrane layer is one of a dropping coating method, a spin coating method, a machine spotting method, or a machine slip film method.
The invention uses the conductive substrate, the air permeable film layer and the electrolyte layer to prepare the dissolved oxygen electrochemical sensor suitable for the micro-fluidic chip and realizes the detection of the dissolved oxygen in the electrolyte solution. The conductive substrate can be a commercial screen printing electrode, a magnetron sputtering electrode, a spraying electrode, an evaporation electrode, a chemical plating electrode, an electrode made of common materials including gold, silver, platinum and carbon, and the dissolved oxygen electrochemical sensor can be cut into any size, shape and thickness according to the design of a micro-fluidic chip needing to be matched; the dissolved oxygen electrochemical sensor is an all-solid-state electrode after being prepared, the storage is convenient, the preparation process of the electrode is simple, and the operability is strong; after the solution of the breathable film layer and the solution of the electrolyte film layer of the dissolved oxygen electrochemical sensor are prepared, the solution can be stored at room temperature for a long time, so that the cost is saved; the dissolved oxygen electrochemical sensor prepared by using the conductive substrates made of different insulating materials and conductive materials has good linear response when the concentration of dissolved oxygen is within the range of 1-20 mg/L.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It should be apparent that the drawings in the following description are merely exemplary, and that other embodiments can be derived from the drawings provided by those of ordinary skill in the art without inventive effort.
The structures, ratios, sizes, and the like shown in the present specification are only used for matching with the contents disclosed in the specification, so as to be understood and read by those skilled in the art, and are not used to limit the conditions that the present invention can be implemented, so that the present invention has no technical significance, and any structural modifications, changes in the ratio relationship, or adjustments of the sizes, without affecting the effects and the achievable by the present invention, should still fall within the range that the technical contents disclosed in the present invention can cover.
FIG. 1 is a schematic structural diagram of a dissolved oxygen electrochemical sensor for a microfluidic chip according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of a dissolved oxygen electrochemical sensor provided in an embodiment of the present invention in combination with a microfluidic chip;
FIG. 3 is a graph of the operation of the electrochemical sensor using dissolved oxygen;
FIG. 4 is a graph of the operation of a dissolved oxygen electrochemical sensor using a magnetron sputtered gold electrode based on a silicon plate as a conductive substrate;
fig. 5 is a graph showing the operation of a dissolved oxygen electrochemical sensor using a screen-printed silver carbon electrode based on PET as a conductive substrate.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
Example 1
Referring to fig. 1, a dissolved oxygen electrochemical sensor for a microfluidic chip is provided, which includes a conductive substrate 5 formed by an insulating material 1 and a conductive material 2, wherein the conductive material 2 covers the surface of the insulating material 1, the surface of the conductive material 2 is covered by an electrolyte layer 3, and the surface of the electrolyte layer 3 is covered by a gas permeable membrane layer 4.
Specifically, the insulating material 1 of the conductive substrate 5 is a silicon plate, PET or PMMA; the conductive material 2 of the conductive substrate 5 is gold, platinum, carbon or silver. The conductive substrate 5 can be cut into any size and shape suitable for the microfluidic chip according to the microfluidic chip, the thickness of the conductive substrate 5 can be as thin as 0.2-0.5 mm, and the conductive substrate can be designed into the microfluidic chip suitable for the sample channel with the width of 0.5-2 mm.
Specifically, the electrolyte layer 3 is gelatin, the gelatin is skin gelatin or bone gelatin, and the gelatin strength of the gelatin is 100-300 g Bloom. The air-permeable film layer 4 is made of a material which is permeable to air, and the air-permeable film layer 4 is made of one of polyvinyl chloride with a K-value of 55-72, organic siloxane and cellulose acetate with an acetyl content of 37% -42%. The conductive substrate 5 adopts one of a screen printing electrode, a magnetron sputtering electrode, a spraying electrode, an evaporation electrode or a chemical plating electrode.
Example 2
The embodiment of the invention provides a preparation method of a dissolved oxygen electrochemical sensor for a microfluidic chip, which comprises the following steps:
weighing a certain amount of PVC, adding the PVC into cyclohexanone under the condition of magnetic stirring, heating and stirring for 2 hours in a water bath at 50 ℃ to completely dissolve the PVC, and preparing 5 percent PVC cyclohexanone solution, namely the breathable film layer 4 solution. Meanwhile, a certain amount of gelatin is weighed and added into 0.1M KCl water solution under the condition of magnetic stirring, and the mixture is heated and stirred for 1 hour in a water bath at 50 ℃ to be completely dissolved to prepare 10 percent gelatin electrolyte solution. And after the solution is prepared, taking the conductive substrate 5, uniformly dripping a proper amount of the gelatin electrolyte layer 3 solution on an electrode detection area, and then drying the electrode on which the gelatin electrolyte layer 3 is dripped in an oven at 37 ℃ for 3 hours. And taking out the dried electrode, placing the dried electrode to room temperature, uniformly covering a proper amount of the solution of the breathable film layer 4 on the surface of the gelatin electrolyte layer 3, and placing the electrode in a fume hood at the room temperature of 20 ℃ for drying for 4 hours to obtain the dissolved oxygen electrochemical sensor for the microfluidic chip. The concrete structure is shown in figure 1. The schematic diagram of the sensor on the microfluidic chip is shown in fig. 2, but fig. 2 is only a partial shape, and the sensor can be designed and cut into any size and shape according to the requirement.
Specifically, the method of uniformly coating the electrolyte layer 3 solution on the conductive substrate 5 is one of a dropping coating method, a spin coating method, a machine spotting method, or a machine slip film method; the method of uniformly coating the solution of the air-permeable film layer 4 on the surface of the electrolyte layer 3 is one of a drop coating method, a spin coating method, a machine spotting method, or a machine slip coating method.
Current value measurement method: pure oxygen was introduced into 50mL of 0.01M PBS solution at room temperature to prepare an oxygen-saturated solution having a dissolved oxygen concentration of 19.8 mg/L. And then introducing nitrogen into the solution to adjust the concentration of the dissolved oxygen so as to obtain dissolved oxygen solutions with different concentrations. The working electrode, the auxiliary electrode and the reference electrode were placed in dissolved oxygen solutions of different concentrations, and the current value was detected by applying a reduction voltage to Chenhua CHI660e, which was an electrochemical workstation. Fig. 3 shows the operating curves obtained by this method, and it can be seen from fig. 3 that the electrode has a good linear response (R2 ═ 0.9863) to the dissolved oxygen concentration in the range of 1.2 to 19.8 mg/L.
Example 3
Preparing a dissolved oxygen electrochemical sensor by taking a magnetron sputtering gold electrode with a silicon plate as an insulating material 1 as a conductive substrate 5 and characterizing the properties of the electrode.
A commercial magnetron sputtering gold electrode with a silicon plate as a substrate is taken and added into cyclohexanone under the condition of magnetic stirring, and the mixture is heated and stirred for 1 hour in a water bath at 55 ℃ to be completely dissolved, so that 15 percent of PVC cyclohexanone solution is prepared, namely the breathable film layer 4 solution. Meanwhile, a certain amount of gelatin is weighed and added into 0.5M KCl water solution under the condition of magnetic stirring, and the mixture is heated and stirred for 0.7 hour in a water bath at 55 ℃ to be completely dissolved, so that 15 percent gelatin electrolyte solution is prepared. And after the solution is prepared, taking the conductive substrate 5, uniformly dripping a proper amount of the gelatin electrolyte layer 3 solution on an electrode detection area, and then drying the electrode on which the gelatin electrolyte layer 3 is dripped in an oven at 37 ℃ for 4 hours. And taking out the dried electrode, placing the dried electrode to room temperature, uniformly covering a proper amount of the solution of the breathable film layer 4 on the surface of the gelatin electrolyte layer 3, and placing the electrode in a fume hood at the room temperature of 25 ℃ for drying for 4 hours to obtain the dissolved oxygen electrochemical sensor for the microfluidic chip. The concrete structure is shown in figure 1. The schematic diagram of the sensor on the microfluidic chip is shown in fig. 2, but fig. 2 is only a partial shape, and the sensor can be designed and cut into any size and shape according to the requirement.
Specifically, the method of uniformly coating the electrolyte layer 3 solution on the conductive substrate 5 is one of a dropping coating method, a spin coating method, a machine spotting method, or a machine slip film method; the method of uniformly coating the solution of the air-permeable film layer 4 on the surface of the electrolyte layer 3 is one of a drop coating method, a spin coating method, a machine spotting method, or a machine slip coating method.
Current value measurement method: pure oxygen was introduced into 50mL of 0.01M PBS solution at room temperature to prepare an oxygen-saturated solution having a dissolved oxygen concentration of 20 mg/L. And then introducing nitrogen into the solution to adjust the concentration of the dissolved oxygen so as to obtain dissolved oxygen solutions with different concentrations. The working electrode, the auxiliary electrode and the reference electrode were placed in dissolved oxygen solutions of different concentrations, and the current value was detected by applying a reduction voltage to Chenhua CHI660e, which was an electrochemical workstation. FIG. 3 is a graph showing the operation curves obtained by the above method, and it can be seen from FIG. 3 that the electrode has a good linear response (R) to the dissolved oxygen concentration in the range of 1.9-20 mg/L2＝0.9962)。
Example 4
And (3) preparing a dissolved oxygen electrochemical sensor by taking a screen printing carbon silver electrode with PET as an insulating material 1 as a conductive substrate 5 and characterizing the electrode properties.
Weighing a certain amount of PVC from a commercial screen printing carbon silver electrode taking PET as an insulating material 1, adding the PVC into cyclohexanone under the condition of magnetic stirring, heating and stirring for 0.5 hour in a water bath at 60 ℃ to completely dissolve the PVC, and preparing the 20% PVC cyclohexanone solution, namely the breathable film layer 4 solution. Meanwhile, a certain amount of gelatin is weighed and added into 1MKCl aqueous solution under the condition of magnetic stirring, and the gelatin is heated and stirred for 0.5 hour in a water bath at 60 ℃ to be completely dissolved to prepare 20 percent gelatin electrolyte solution. And after the solution is prepared, taking the conductive substrate 5, uniformly dripping a proper amount of the gelatin electrolyte layer 3 solution on an electrode detection area, and then drying the electrode on which the gelatin electrolyte layer 3 is dripped in an oven at 37 ℃ for 3 hours. And taking out the dried electrode, placing the dried electrode to room temperature, uniformly covering a proper amount of the solution of the breathable film layer 4 on the surface of the gelatin electrolyte layer 3, and placing the electrode in a fume hood at the room temperature of 25 ℃ for drying for 4 hours to obtain the dissolved oxygen electrochemical sensor for the microfluidic chip. The concrete structure is shown in figure 1. The schematic diagram of the sensor on the microfluidic chip is shown in fig. 2, but fig. 2 is only a partial shape, and the sensor can be designed and cut into any size and shape according to the requirement.
Specifically, the method of uniformly coating the electrolyte layer 3 solution on the conductive substrate 5 is one of a dropping coating method, a spin coating method, a machine spotting method, or a machine slip film method; the method of uniformly coating the solution of the air-permeable film layer 4 on the surface of the electrolyte layer 3 is one of a drop coating method, a spin coating method, a machine spotting method, or a machine slip coating method.
Current value measurement method: pure oxygen was introduced into 50mL of 0.01M PBS solution at room temperature to prepare an oxygen-saturated solution having a dissolved oxygen concentration of 20.3 mg/L. And then introducing nitrogen into the solution to adjust the concentration of the dissolved oxygen so as to obtain dissolved oxygen solutions with different concentrations. The working electrode, the auxiliary electrode and the reference electrode were placed in dissolved oxygen solutions of different concentrations, and the current value was detected by applying a reduction voltage to Chenhua CHI660e, which was an electrochemical workstation. FIG. 4 is a graph showing the operation curves obtained by the method, and it can be seen from FIG. 4 that the electrode has a good linear response (R) to the dissolved oxygen concentration in the range of 1.1-20.3 mg/L2＝0.9968)。
The invention uses the conductive substrate, the air permeable film layer and the electrolyte layer to prepare the dissolved oxygen electrochemical sensor suitable for the micro-fluidic chip and realizes the detection of the dissolved oxygen in the electrolyte solution. The conductive substrate 5 can be a commercial screen printing electrode, a magnetron sputtering electrode, a spraying electrode, an evaporation electrode, a chemical plating electrode, an electrode made of common materials including gold, silver, platinum and carbon, and the dissolved oxygen electrochemical sensor can be cut into any size, shape and thickness according to the design of a micro-fluidic chip needing to be matched; the dissolved oxygen electrochemical sensor is an all-solid-state electrode after being prepared, the storage is convenient, the preparation process of the electrode is simple, and the operability is strong; the solution of the air-permeable film layer 4 and the solution of the electrolyte film layer of the dissolved oxygen electrochemical sensor can be stored for a long time at room temperature after being prepared, so that the cost is saved; the dissolved oxygen electrochemical sensor prepared by using the conductive substrate 5 made of different insulating materials 1 and conductive materials 2 has good linear response within the dissolved oxygen concentration range of 1-20 mg/L.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. The dissolved oxygen electrochemical sensor for the micro-fluidic chip is characterized by comprising a conductive substrate formed by an insulating material and a conductive material, wherein the conductive material covers the surface of the insulating material, an electrolyte layer covers the surface of the conductive material, and a breathable film layer covers the surface of the electrolyte layer.
2. The dissolved oxygen electrochemical sensor for the microfluidic chip according to claim 1, wherein the insulating material of the conductive substrate is silicon plate, PET or PMMA; the conductive material of the conductive substrate is gold, platinum, carbon or silver.
3. The dissolved oxygen electrochemical sensor for the microfluidic chip according to claim 1, wherein the electrolyte layer is gelatin, the gelatin is skin gelatin or bone gelatin, and the gelatin has a gel strength of 100-300 g Bloom.
4. The dissolved oxygen electrochemical sensor for the microfluidic chip according to claim 1, wherein the gas permeable membrane layer is made of a material having permeability to gas, and the gas permeable membrane layer is made of one of polyvinyl chloride with a K-value of 55-72, organosiloxane and cellulose acetate with an acetyl content of 37-42%.
5. The dissolved oxygen electrochemical sensor for a microfluidic chip according to claim 1, wherein the conductive substrate is one of a screen-printed electrode, a magnetron sputtering electrode, a spray electrode, an evaporation electrode or a chemical plating electrode.
6. A preparation method of a dissolved oxygen electrochemical sensor for a microfluidic chip is characterized by comprising the following steps:
preparing electrolyte layer solution in advance, weighing quantitative gelatin, adding the gelatin into the prepared electrolyte aqueous solution under the condition of magnetic stirring, and stirring for 0.5-1 hour by magnetic force to completely dissolve the gelatin in the electrolyte aqueous solution to prepare 10-20% electrolyte layer solution;
uniformly covering the electrolyte layer solution on a conductive substrate, and drying in an oven at 37 ℃ for more than 3 hours;
preparing a breathable film layer solution in advance, weighing a certain amount of breathable film material, adding the breathable film material into an organic solvent under the condition of magnetic stirring, and stirring for 0.5-2 hours by magnetic force to completely dissolve the breathable film material in the organic solvent to prepare a 5-20% breathable film layer solution;
and uniformly covering the surface of the electrolyte layer with the solution of the breathable film layer, and airing in a fume hood at the temperature of 20-25 ℃ for 0.5-4 hours to prepare the dissolved oxygen electrochemical sensor suitable for the microfluidic chip.
7. The method for preparing a dissolved oxygen electrochemical sensor for a microfluidic chip according to claim 6, wherein the organic solvent of the gas permeable membrane layer solution is cyclohexanone, propiophenone, chloroform or acetone.
8. The method for preparing a dissolved oxygen electrochemical sensor for a microfluidic chip according to claim 6, wherein the electrolyte aqueous solution is a KCl solution of 0.1mol/L, 0.5mol/L or 1mol/L, or a KNO solution of 0.1mol/L, 0.5mol/L or 1mol/L3And (3) solution.
9. The method of claim 6, wherein the method of uniformly coating the electrolyte layer solution on the conductive substrate is one of a dropping method, a spin coating method, a machine spotting method, or a machine slip film method.
10. The method of claim 6, wherein the method of uniformly coating the electrolyte layer with the solution of the gas permeable film layer is one of a dropping method, a spin coating method, a machine spotting method, or a machine slip film method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911187616.3A CN110887885A (en) | 2019-11-28 | 2019-11-28 | Dissolved oxygen electrochemical sensor for micro-fluidic chip and preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911187616.3A CN110887885A (en) | 2019-11-28 | 2019-11-28 | Dissolved oxygen electrochemical sensor for micro-fluidic chip and preparation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110887885A true CN110887885A (en) | 2020-03-17 |
Family
ID=69749139
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911187616.3A Pending CN110887885A (en) | 2019-11-28 | 2019-11-28 | Dissolved oxygen electrochemical sensor for micro-fluidic chip and preparation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110887885A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111948275A (en) * | 2020-08-27 | 2020-11-17 | 江苏农林职业技术学院 | Dissolved oxygen detection device based on micro-fluidic chip |
| CN115586234A (en) * | 2022-12-12 | 2023-01-10 | 艾康生物技术(杭州)有限公司 | Biosensor and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070256944A1 (en) * | 2006-04-14 | 2007-11-08 | Tamkang University | Method and electrochemical sensing strip with screen-printed three electrodes for determining concentration of dissolved oxygen in a solution |
| CN101881749A (en) * | 2010-06-25 | 2010-11-10 | 浙江大学 | All-solid state dissolved oxygen sensor and preparation method thereof |
| CN102353711A (en) * | 2011-10-18 | 2012-02-15 | 郑州炜盛电子科技有限公司 | Primary-cell-type semisolid dissolved oxygen sensor |
| CN107102042A (en) * | 2017-04-11 | 2017-08-29 | 中国农业大学 | A kind of screen printing type dissolved oxygen electrode preparation method and dissolved oxygen electrode |
| CN109868220A (en) * | 2017-12-05 | 2019-06-11 | 中国科学院苏州纳米技术与纳米仿生研究所 | A kind of micro-fluidic dissolved oxygen detection chip and its application |
| CN110243914A (en) * | 2019-06-21 | 2019-09-17 | 南京晶捷生物科技有限公司 | A kind of all solid state electrochemistry macromolecule sensor measuring dissolved oxygen |
-
2019
- 2019-11-28 CN CN201911187616.3A patent/CN110887885A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070256944A1 (en) * | 2006-04-14 | 2007-11-08 | Tamkang University | Method and electrochemical sensing strip with screen-printed three electrodes for determining concentration of dissolved oxygen in a solution |
| CN101881749A (en) * | 2010-06-25 | 2010-11-10 | 浙江大学 | All-solid state dissolved oxygen sensor and preparation method thereof |
| CN102353711A (en) * | 2011-10-18 | 2012-02-15 | 郑州炜盛电子科技有限公司 | Primary-cell-type semisolid dissolved oxygen sensor |
| CN107102042A (en) * | 2017-04-11 | 2017-08-29 | 中国农业大学 | A kind of screen printing type dissolved oxygen electrode preparation method and dissolved oxygen electrode |
| CN109868220A (en) * | 2017-12-05 | 2019-06-11 | 中国科学院苏州纳米技术与纳米仿生研究所 | A kind of micro-fluidic dissolved oxygen detection chip and its application |
| CN110243914A (en) * | 2019-06-21 | 2019-09-17 | 南京晶捷生物科技有限公司 | A kind of all solid state electrochemistry macromolecule sensor measuring dissolved oxygen |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111948275A (en) * | 2020-08-27 | 2020-11-17 | 江苏农林职业技术学院 | Dissolved oxygen detection device based on micro-fluidic chip |
| CN115586234A (en) * | 2022-12-12 | 2023-01-10 | 艾康生物技术(杭州)有限公司 | Biosensor and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Schaffar | Thick film biosensors for metabolites in undiluted whole blood and plasma samples | |
| CN1184470C (en) | Bi-mediator-based glucose biosensor and its use method | |
| CN110887885A (en) | Dissolved oxygen electrochemical sensor for micro-fluidic chip and preparation method | |
| CN104931557B (en) | A kind of production method of electrochemistry formaldehyde sensor and its electrode | |
| CN109298053A (en) | A method of glucose is measured using AuNPs/AgNWs/PDMS ductile electrode | |
| CN104569088A (en) | Test paper strip for blood detection by electrochemical process and production method of test paper strip | |
| CN106324059B (en) | A kind of preparation method of glucose sensor electrode without enzyme material | |
| CN105963754A (en) | Double-layer polysiloxane super-molecular elastomer dressing for chronic skin wounds and preparation method thereof | |
| CN109142490A (en) | All-solid sodium ion selective sensor and its preparation and application | |
| JP3149597B2 (en) | Body fluid component measurement device | |
| CN1710418A (en) | Biological sensor for quickly detecting hemoglobin and detecting method thereof | |
| TWI329738B (en) | ||
| CN109991298B (en) | Preparation method and application of Pt @ MOF-GO labeled electrochemical sensor | |
| Hirose et al. | Polyvinylhcloride membrane for a glucose sensor | |
| CN101140256B (en) | Preparation method of photofixation horse radish peroxidase electric pole and uses thereof | |
| EP4184170A1 (en) | Device for detecting blood coagulation characteristics and application of device | |
| CN109406596A (en) | A kind of electrochemical sensor, preparation method and application detecting microRNA-21 | |
| CN106039381B (en) | A kind of photocuring bilayer polysiloxanes supermolecule elastomer dressing and preparation method thereof for chronic cutaneous wound | |
| CN110243914B (en) | All-solid-state electrochemical polymer sensor for measuring dissolved oxygen | |
| CN106483182A (en) | A kind of dry type determines biology sensor of hemoglobin and preparation method thereof | |
| CN208721608U (en) | A kind of electrochemistry test paper of rapid survey amylase | |
| CN111671427A (en) | Inverse spinel type Co2SnO4YSZ-based mixed potential type H as sensitive electrode2S sensor and preparation method thereof | |
| EP3404418A2 (en) | A diagnostic strip for determining the amount of sarcosine, creatinine and hydrogen peroxide in a biological or environmental sample | |
| TW200407542A (en) | Electro-chemical electrode sensing test sheet for screen printing and its manufacturing method | |
| CN217305001U (en) | In-vitro human subcutaneous physiological environment simulation test system for testing implantable sensor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information |
Address after: 102200, Beijing Changping District science and Technology Park, super Road, No. 37 Applicant after: Beijing Lepu Diagnostic Technology Co.,Ltd. Address before: 102200, Beijing, Changping District, super Road, No. 7-1, building 37 Applicant before: BEIJING LEPU MEDICAL TECHNOLOGY Co.,Ltd. |
|
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200317 |