CN110885857A - Intermittent flow type formula electrotransfection device - Google Patents
Intermittent flow type formula electrotransfection device Download PDFInfo
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- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
Abstract
The invention discloses an intermittent flow type electrotransfection device, which comprises a tube body, a first electrode and a second electrode, and is characterized in that the first electrode and the second electrode are positioned at the tube orifices at two ends of the tube body in parallel, and a cavity for containing a target liquid sample is formed in the tube body, the first electrode and the second electrode; the tube body is provided with at least one liquid inlet and outlet and at least one liquid inlet and outlet valve, and the liquid inlet and outlet valve is positioned at the liquid inlet and outlet of the tube body and used for controlling the opening and closing of the liquid inlet and outlet; the first electrode and/or the second electrode can move relatively along the tube. The intermittent flow type electrotransfection device disclosed by the invention avoids bubbles generated in the electrotransfection process by utilizing pressure, improves the electrotransfection efficiency and realizes the rapid treatment of a large number of samples.
Description
Technical Field
The invention relates to the field of electrotransfection, in particular to an intermittent flow type electrotransfection device, which inhibits bubbles generated by electrotransfection in a mode of moving and pressurizing electrodes.
Background
The cell membrane is a thin membrane surrounding the cell periphery and is a permeable barrier for selective exchange of substances between the cell and the outside. The cell membrane makes the cell an independent life unit and has a relatively stable internal environment. Some substances in the surrounding environment may pass through the cell membrane, others do not. Cells can take up nutrients from the surrounding environment through the cell membrane, excrete metabolites, and allow the transport of substances to reach an equilibrium state. Therefore, the basic function of the cell membrane is to maintain a relatively stable intracellular microenvironment and selectively exchange substances with the external environment.
It is found that if a certain intensity of electric stimulation is applied to cells for a certain period of time, micropores can be induced on cell membranes, so that the permeability of the cells is enhanced, and the cell Electroporation (Electroporation) refers to a biophysical process of the cells under the action of an applied pulse electric field, wherein transient micropores are formed on cell membrane lipid bilayers. Electrotransfection (Electrotransfection) is a technique for introducing foreign biological macromolecules, such as DNA, RNA or proteins, into cells using electroporation. When the cell membrane is subjected to electroporation, the permeability and the membrane conductance of the cell membrane are increased instantaneously, so that molecules, such as hydrophilic molecules, DNA, proteins, virus particles, drug particles and the like, which cannot pass through the cell membrane under normal conditions can enter the cell through micropores. After the electrical stimulation is removed in a short time, the micropores in the cell membrane disappear, and the cell membrane becomes a selective permeability barrier again. Compared with the traditional chemical transfection and virus transfection, the electrotransfection has the advantages of no chemical pollution, no permanent damage to cells, high efficiency and the like, and has wide application prospect in the fields of biophysics, molecular biology, clinical medicine and the like.
Although the mechanism of electrotransfection is not completely understood, it is well known in this context that cell electrotransfection involves the movement of the lipid bilayer of the cell membrane, resulting in the formation of transient micropores in the membrane, allowing exogenous molecules to enter the cell through the micropores.
In the prior art, there are three main types of methods for completing the process of electrotransfection of cells:
the cells are placed between a pair of parallel electrodes spaced a few millimeters to a few centimeters apart. The cells are electrically stimulated in an electric field between the electrodes for the purpose of electrotransfection. For example, US patent No. 5389069.
The micro needle electrode is pricked into tissue or cell fluid to shock the cell electrically to reach the aim of electrotransfection. For example, US patent No. 5389069.
A chamber is placed between a pair of parallel electrodes so that a suspension solution of cells is electrically shocked while flowing in the chamber. For example, US patent US 6773669.
Chinese patent CN201010242144 discloses a flow electrotransfection device and system, the system includes: a flow electrotransfection device comprising: the electrode is arranged in parallel and in pairs, and each pair of electrodes comprises an anode and a cathode which are oppositely arranged; a channel disposed over the electrode that restricts fluid flow; the starting end of the channel is provided with a plurality of inlet branch channels which are converged into a main channel, the ending end of the channel is provided with a plurality of outlet branch channels, and a top cover with a plurality of fluid inlets and outlets is arranged above the channel; the injection pump is connected to the inlet and the outlet of the top cover in the flow type electrotransfection device through pipelines to control the flow rate of the fluid; and the voltage source is connected with the electrode set by the electric connector and generates pulse voltage. The flow-type electrotransfection system utilizes the fluid channel and the connected injection pump to realize the continuous flow of various suspensions in the fluid channel, thereby enabling the process that the cells are electrotransfected to be continuously carried out and realizing the rapid processing of a large number of samples.
Chinese patent CN201610806987 discloses a disposable article for the electrotransfection of cells comprising: a fluid compartment inside the disposable; a first fluid port for providing a cell suspension to the fluid compartment; and a second fluid port for delivering a fluid comprising at least one compound to be electrotransfected into the cell to the fluid compartment; a first electrode and a second electrode disposed in the fluid compartment; at least one outlet port delivering the fluid from the fluid compartment, wherein the first and second fluid ports are in fluid communication with a mixing channel in fluid communication with the fluid compartment.
However, the flow electrotransfection device disclosed above generates a large amount of bubbles during the actual electrotransfection process, and the generated bubbles adhere to the electrode plates to affect the uniformity of the electric field, thereby causing the electrotransfection effect to be unstable. The inventor finds that the technical problem of bubble generation in the electrotransfection process is not solved in the field of flow electrotransfection.
Disclosure of Invention
The invention provides an intermittent flow type electrotransfection device which can inhibit bubbles from being generated in the electrotransfection process in a movable electrode pressurization mode aiming at the technical problems in the prior art. It is another object of the present invention to improve the stability of electrotransfection devices and to improve the efficiency of electrotransfection.
In order to achieve the above object, the technical scheme of the intermittent flow type electrotransfection device provided by the invention is summarized as follows: an intermittent flow type electrotransfection device comprises a tube body, a first electrode and a second electrode, and is characterized in that the first electrode and the second electrode are positioned in parallel at pipe orifices at two ends of the tube body, and a cavity for containing a target liquid sample is formed inside the tube body, the first electrode and the second electrode; the tube body is provided with at least one liquid inlet and outlet and at least one liquid inlet and outlet valve, and the liquid inlet and outlet valve is positioned at the liquid inlet and outlet of the tube body and used for controlling the opening and closing of the liquid inlet and outlet; the first electrode and/or the second electrode can move relatively along the tube.
Preferably, the body sets up the breather pipe, and the breather pipe sets up the breather valve, and further preferably the breather pipe sets up the breather valve with the body junction, the breather pipe is located between first electrode and the second electrode, the end of breather pipe sets up air filter equipment.
Preferably, the tube body is provided with at least two liquid inlets and outlets, the liquid inlets and outlets can be liquid inlets or liquid outlets, and at least two liquid inlet and outlet valves are correspondingly arranged; the liquid inlet and outlet valve can be a liquid sample inlet one-way valve and can also be a liquid sample outlet one-way valve; further preferably, the tube body is provided with at least one liquid inlet and one liquid outlet, at least one liquid sampling one-way valve and one liquid sampling one-way valve;
preferably, the cross section of the first electrode and the cross section of the second electrode are the same as or close to the cross section of the interior of the tube, and further preferably, the cross section of the interior of the tube is square, and can also be round or in other shapes;
preferably, sealing devices are arranged at the contact positions of the first electrode and the second electrode and the tube body, and the sealing devices are preferably sealing gaskets, sealing rubber strips or other devices with sealing performance;
preferably, when the liquid inlet and outlet valve and the vent valve are closed, the tube body and the interiors of the first electrode and the second electrode form a closed cavity;
preferably, when the liquid inlet/outlet valve and the vent valve are closed, and the first electrode and/or the second electrode move relative to the tube body, the pressure value inside the closed cavity is 1.0-2.5 Mpa; further preferably, the pressure value in the cavity is 1.1-2.0 Mpa; further preferably, the pressure value in the cavity is 1.2-1.5 Mpa;
preferably, the device also comprises a plurality of guide pipes which are divided into a liquid inlet pipe and a liquid outlet pipe, and the guide pipes and the pipe body are arranged in a sealing way;
preferably, the device further comprises a pump for flowing liquid out of or into the cavity or for evacuating or filling gas in the cavity, the pump being of the kind including but not limited to peristaltic pump, air pump, liquid pump, syringe pump, etc.;
preferably, the device further comprises a pressurizing device which enables the first electrode and/or the second electrode to move relatively along the tube body, and the pressurizing device is of a type including but not limited to a balloon, a hydraulic rod, a pneumatic rod, a screw pressurizing device, an electromagnetic force pressurizing device and the like;
preferably, the first electrode and/or the second electrode are provided with a working position and a non-working position, and the electrode is moved from the non-working position to the working position to provide pressure for the inside of the cavity; it is further preferred that the liquid inlet/outlet is located between the first and second electrodes. It is further preferred that the fluid inlet or outlet is located between an operative position in which the first and/or second electrode is disposed and an inoperative position.
Preferably, the second electrode is fixedly arranged at a pipe orifice at one end of the pipe body.
The preparation method of the device comprises the steps of firstly preparing a tube body, selecting plastic capable of being solidified after heating as a material of the tube body, preparing the tube body by utilizing a mould for preparing the tube body, respectively arranging a liquid sample inlet and a liquid sample outlet on the tube body, respectively arranging two opposite parallel electrodes at two ends of the tube body, wherein the electrodes are two metal sheets with the same shape and size, and are injected into a plastic frame and are a first electrode and a second electrode. One side of the electrode, which is not in contact with the cavity, is connected with a pressurizing device, and flexible adhesive tapes are arranged around the electrode plastic frame. Fixedly mounting the second electrode, and connecting the first electrode with a pressurizing device to enable the first electrode to be displaced under the pressure generated by the pressurizing device; or the first electrode and the second electrode are respectively connected with the pressurizing device, so that the first electrode and the second electrode can be displaced under the pressure generated by the pressurizing device.
The technical scheme of the invention has the following beneficial effects:
the intermittent flow electrotransfection device of the present invention is capable of processing large volumes of liquid samples.
The intermittent flow type electrotransfection device pressurizes liquid in the electrotransfection process, inhibits bubbles from generating, reduces electrotransfection efficiency fluctuation caused by bubble attachment, and improves the stability of the electrotransfection device.
The intermittent flow type electrotransfection device has novel structural design and is pioneering.
Drawings
FIG. 1 is an exemplary diagram of an intermittent flow electrotransfection apparatus of the present invention;
FIG. 2 is an exemplary diagram of an intermittent flow electrotransfection apparatus of the present invention;
the attached drawings are annotated: 1-a first electrode, 2-a second electrode, 3-a tube body, 4-a liquid inlet tube, 5-a liquid outlet tube, 6-a pressurizing device, 7-a first electrode non-working position, 8-a first electrode working position, 9-a second electrode non-working position, 10-a second electrode working position, 11-a sample introduction one-way valve, 12-a sample discharge one-way valve, 13-a vent tube and 14-a vent valve.
Detailed description of the preferred embodiments
Example one
The integral structure of the intermittent flow type electrotransfection device is shown in figure 1, the two ends of the pipe body (3) are sealed, wherein one end of the pipe opening adopts the second electrode (2) as the sealing arrangement, the other end of the pipe opening adopts the first electrode (1) as the sealing arrangement, the pipe body (3) is provided with a vent pipe (13), and the joint of the vent pipe (13) and the pipe body (3) is provided with a vent valve (14). The first electrode (1) is provided with a working position (8) and a non-working position (7) in the tube body (3), the first electrode (1) is connected with the pressurizing device (6), the pressurizing device (6) is preferably a hydraulic rod, and the pressurizing device (6) can push the first electrode (1) to move from the non-working position (7) to the working position (8), so that liquid carried in the cavity bears certain pressure. And applying electric pulses to the first electrode (1) and the second electrode (2), wherein the electric pulses enable an electric field to be formed in the cavity, so that the cell membranes form micropores, and the target substances in the liquid sample can enter the cells. After completion of the electrotransfection, the pressurizing means (6) are able to pull the first electrode (1) from the working position (8) back to the non-working position (7). The above procedure was repeated to perform intermittent flow electrotransfection.
Example two
The integral structure of the intermittent flow type electrotransfection device is shown in figure 2, the end closures of pipe orifices at two ends of the pipe body (3) are respectively sealed by a first electrode (1) and a second electrode (2), wherein the first electrode (1) and the second electrode (2) are connected with a pressurizing device (6), and the pressurizing device (6) is preferably a hydraulic rod. The pipe body (3) is provided with a vent pipe (13), and a vent valve (14) is arranged at the joint of the vent pipe (13) and the pipe body (3). The first electrode (1) is provided with a working position (8) and a non-working position (7) in the tube body (3), and the second electrode (2) is provided with a working position (10) and a non-working position (9) in the tube body. According to the relative movement of the first electrode (1) and the second electrode (2), the following three operation states can be set.
In the first working state, the pressurizing devices (6) at the two end orifices push the first electrode (1) and the second electrode (2) to move from the non-working positions (7) and (9) to the working positions (8) and (10), so that the liquid in the cavity bears certain pressure, at the moment, electric pulses are applied to the first electrode (1) and the second electrode (2), the electric pulses form an electric field in the cavity, and micropores are formed on cell membranes, so that target substances in the liquid sample can enter cells. After the electrotransfection is completed, the pressurizing device (6) can pull the first electrode (1) and the second electrode (2) from the working position to the non-working position.
In the second working state, the second electrode (2) is fixed at the non-working position (9), the pressurizing device (6) pushes the first electrode (1) to move from the non-working position (7) to the working position (8), so that the liquid in the cavity bears certain pressure, at the moment, electric pulses are applied to the first electrode (1) and the second electrode (2), the electric pulses form an electric field in the cavity, and micropores are formed in cell membranes, so that target substances in the liquid sample can enter the cells. After completion of the electrotransfection, the pressurizing means (6) are able to pull the first electrode (1) from the working position (8) back to the non-working position (7).
And in the third working state, the first electrode (1) is fixed at the non-working position (7), the pressurizing device (6) pushes the second electrode (2) to move from the non-working position (9) to the working position (10), so that the liquid in the cavity bears certain pressure, electric pulses are applied to the first electrode (1) and the second electrode (2), the electric pulses form an electric field in the cavity, the cell membrane has permeability, and therefore the target substance in the liquid sample can enter the cell. After completion of the electrotransfection, the pressurizing means (6) are able to pull the second electrode (2) from the working position (10) back to the non-working position (9).
EXAMPLE III
Intermittent liquid feeding mode
When liquid is fed, the sample inlet one-way valve (11) is opened, the sample outlet one-way valve (12) is closed, the vent valve (14) is opened, gas with the same volume as the tube body (3) is pumped out through the vent pipe (13) by an air pump, and a target liquid sample enters the cavity from the liquid inlet tube (4); or the air pump is not used for pumping the air with the same volume with the tube body (3) through the vent pipe (13), and the peristaltic pump is used for pressing the target liquid sample into the cavity from the liquid inlet pipe (4); after the cavity is filled, the sample injection one-way valve (11) and the vent valve (14) are closed.
After electrotransfection is finished, opening a sample outlet one-way valve (12) and a vent valve (14), filling gas with the same volume as the cavity into the tube body through the vent valve (14) by using an air pump, and discharging a target liquid sample from the liquid outlet pipe (5); or the air pump is not used, the air with the same volume as the tube body is filled into the cavity through the vent tube (14), and the target liquid sample is discharged from the liquid outlet tube (5) by virtue of gravity; after the target liquid sample is completely discharged, the sample outlet one-way valve (12) is closed, the sample inlet one-way valve (11) is opened, and the actions of liquid feeding are repeated.
Example four
Liquid pressurization mode in cavity
After the target liquid enters and fills the cavity through the sample injection one-way valve (11), the sample injection one-way valve (11) is closed, and at the moment, a specific pressure is applied to the first electrode (1) and/or the second electrode (2) through the pressurizing device (6) to enable the first electrode and/or the second electrode to move from the non-working positions (7) and (9) to the working positions (8) and (10), so that the pressurization of the liquid is completed, and the electrotransfection of the liquid is carried out in a pressurized state.
EXAMPLE five
Bioassay system
CHO-S cells (Chinese hamster ovary cells) in logarithmic growth phase were collected, centrifuged at 1000 rpm for 5 minutes, the supernatant discarded, and the cells resuspended in electrotransfection buffer to a cell density of 1X107Adding plasmid pCDNA3.1-GFP to be transfected into cells to make the concentration of the plasmid be 20ug/ml, and mixing gently. The used intermittent flow type electrotransfection device is shown in figure 1, the end closures of pipe orifices at two ends of the pipe body are respectively sealed by a first electrode and a second electrode, and the first electrode is connected with a hydraulic rod. Before liquid enters the cavity, the sample inlet one-way valve is opened, the sample outlet one-way valve is closed, the vent valve is opened, gas with the same volume as the tube body is pumped out through the vent pipe by an air pump, and a target liquid sample enters the cavity from the liquid inlet pipe; and after the cavity is full, closing the sample introduction one-way valve and the vent valve. The first electrode is maintained in a non-operative position and the liquid is not pressurized. At the moment, electric pulses are applied to the first electrode and the second electrode, the pulse voltage is 180 volts, the pulse width is 6 milliseconds, the pulse times are 2 times, the pulse interval is 1 second, electric fields are formed in the cavity through the electric pulses, the cell membranes form micropores, and therefore target substances in the liquid sample can enter the cells. After electrotransfection is completed, the sample outlet one-way valve and the vent valve are opened, gas with the same volume as the cavity is filled into the tube body through the vent pipe by using the air pump, and the target liquid sample is discharged from the liquid outlet pipe. The transfected cell suspension was placed in a centrifuge tube at 1000 rpm and centrifuged for 5 minutes. Discarding supernatant, adding CD-OptiCHO culture medium to suspend cells, inoculating, and culturing in a triangular conical flaskCulturing at a density of 2X 106And/ml, then placing the mixture on a shaking table for culturing, wherein the rotation speed of the shaking table is 125rpm/min, and the culture conditions are as follows: the temperature was 37 ℃ and the carbon dioxide concentration was 5%. After 24 hours, the cells were observed under a fluorescence microscope, and the electrotransfection efficiency and the cell viability were measured by a flow cytometer.
EXAMPLE six
Bioassay (II)
CHO-S cells (Chinese hamster ovary cells) in logarithmic growth phase were collected, centrifuged at 1000 rpm for 5 minutes, the supernatant discarded, and the cells resuspended in electrotransfer buffer to a cell density of 1X107One cell per ml, the plasmid pCDNA3.1-GFP to be transfected into cells was added to a concentration of 20ug/ml, and mixed gently. The used intermittent flow type electrotransfection device is shown in figure 1, the end closures of pipe orifices at two ends of the pipe body are respectively sealed by a first electrode and a second electrode, and the first electrode is connected with a hydraulic rod. Before liquid enters the cavity, the sample inlet one-way valve is opened, the sample outlet one-way valve is closed, the vent valve is opened, gas with the same volume as the tube body is pumped out through the vent pipe by an air pump, and a target liquid sample enters the cavity from the liquid inlet pipe; and after the cavity is full, closing the sample introduction one-way valve and the vent valve. The hydraulic rod pushes the first electrode to move from the non-working position to the working position, so that the liquid in the cavity bears certain pressure. At the moment, electric pulses are applied to the first electrode and the second electrode, the pulse voltage is 180 volts, the pulse width is 6 milliseconds, the pulse times are 2 times, the pulse interval is 1 second, electric fields are formed in the cavity through the electric pulses, the cell membranes form micropores, and therefore target substances in the liquid sample can enter the cells. After electrotransfection is completed, the hydraulic rod can pull the first electrode from the working position back to the non-working position. After electrotransfection is completed, the sample outlet one-way valve and the vent valve are opened, gas with the same volume as the cavity is filled into the tube body through the vent pipe by using the air pump, and the target liquid sample is discharged from the liquid outlet pipe. The transfected cell suspension was placed in a centrifuge tube at 1000 rpm and centrifuged for 5 minutes. Discarding supernatant, adding OptiCHO culture medium to resuspend cells, inoculating, culturing in a triangular conical flask at a culture density of 2 × 106/ml, then placing on a shaking table for culturing, wherein the rotation speed of the shaking table is 125rpm/min, and culturingConditions are as follows: the temperature was 37 ℃ and the carbon dioxide concentration was 5%. After 24 hours, the cells were observed under a fluorescence microscope, and the electrotransfection efficiency and the cell viability were measured by a flow cytometer.
And (3) observing experimental results:
it can be clearly observed in experiment five that a small amount of bubbles appear in the chamber after the pulse, but the chamber in experiment six is still full of liquid and no bubbles are generated.
After 24 hours, the infection rate of the five experimental cells is 61-63 percent, and the cell survival rate is 70-81 percent; the transfection rate of experimental six cells is 83-% 87%, and the cell survival rate is 81% -84%.
In conclusion, the pressurizing cavity provided by the invention can improve the electrotransfection efficiency and has an obvious effect of reducing the generation of bubbles.
Although the present invention has been described in detail, modifications within the spirit and scope of the invention will be apparent to those skilled in the art. It should be understood that aspects of the invention and portions of the various embodiments and various features described above and/or in the appended claims may be combined or interchanged either in whole or in part. As will be appreciated by one skilled in the art, in the foregoing description of the various embodiments, those embodiments referring to another embodiment may be combined with other embodiments as appropriate. Furthermore, those of ordinary skill in the art will appreciate that the foregoing description is by way of example only, and is not intended to limit the invention.
Claims (10)
1. An intermittent flow type electrotransfection device comprises a tube body, a first electrode and a second electrode, and is characterized in that the first electrode and the second electrode are positioned in parallel at pipe orifices at two ends of the tube body, and a cavity for containing a target liquid sample is formed inside the tube body, the first electrode and the second electrode; the tube body is provided with at least one liquid inlet and outlet and at least one liquid inlet and outlet valve, and the liquid inlet and outlet valve is positioned at the liquid inlet and outlet of the tube body and used for controlling the opening and closing of the liquid inlet and outlet; the first electrode and/or the second electrode can move relatively along the tube.
2. An intermittent flow electrotransfection device according to claim 1, wherein the tube is provided with at least two fluid ports.
3. An intermittent flow electrotransfection device according to claim 2, wherein the fluid inlet and outlet is located between the first and second electrodes.
4. An intermittent flow electrotransfection device according to claim 1, wherein the first electrode and/or the second electrode are disposed in an operative position and an inoperative position.
5. An intermittent flow electrotransfection device according to claim 1, wherein the body is provided with a vent tube, the vent tube being provided with a vent valve. Preferably, a vent valve is arranged at the joint of the vent pipe and the pipe body.
6. An intermittent flow electrotransfection device according to claim 1, wherein the first and second electrodes are provided with sealing means, preferably a gasket, bead or other means having sealing properties, where they contact the tubular body.
7. An intermittent flow electrotransfection device according to any one of claims 5 to 6, wherein the tubular body and the interior of the first and second electrodes form a closed chamber when the liquid inlet and outlet valves and the vent valve are closed.
8. An intermittent flow electrotransfection device according to claim 7, wherein the pressure inside the closed chamber is 1.0 to 2.50Mpa, preferably 1.1 to 2.0Mpa, after relative movement of the first and/or second electrode along the tube when the liquid inlet and outlet valves and the vent valve are closed.
9. An intermittent flow electrotransfection device according to claim 1, further comprising a pressurizing means to enable relative movement of the first and/or second electrodes along the tube. Preferably, the pressurizing means includes, but is not limited to, an air bag, a hydraulic rod, a pneumatic rod, a screw pressurizing means, an electromagnetic force pressurizing means, and the like.
10. An intermittent flow electrotransfection device according to any one of claims 1 to 9, wherein the second electrode is fixedly disposed at one end of the tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811054671.0A CN110885857B (en) | 2018-09-11 | 2018-09-11 | Intermittent flow type formula electrotransfection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811054671.0A CN110885857B (en) | 2018-09-11 | 2018-09-11 | Intermittent flow type formula electrotransfection device |
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| Publication Number | Publication Date |
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| CN110885857A true CN110885857A (en) | 2020-03-17 |
| CN110885857B CN110885857B (en) | 2024-01-26 |
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| US20130052711A1 (en) * | 2011-08-25 | 2013-02-28 | Jian Chen | Methods and devices for electroporation |
| US20140220665A1 (en) * | 2010-05-12 | 2014-08-07 | Robert J Walters | Dynamic mixing and electroporation chamber and system |
| CN205205150U (en) * | 2015-12-01 | 2016-05-04 | 陈剑 | Electric shock pipe |
| CN205205151U (en) * | 2015-12-01 | 2016-05-04 | 陈剑 | Electric shock pipe |
| CN106701565A (en) * | 2015-09-07 | 2017-05-24 | 美天施生物科技有限责任公司 | Disposable cartridge for electroporation |
| CN209098693U (en) * | 2018-09-11 | 2019-07-12 | 苏州壹达生物科技有限公司 | A kind of intermittent streaming electrotransfection device |
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Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US20140220665A1 (en) * | 2010-05-12 | 2014-08-07 | Robert J Walters | Dynamic mixing and electroporation chamber and system |
| US20130052711A1 (en) * | 2011-08-25 | 2013-02-28 | Jian Chen | Methods and devices for electroporation |
| CN106701565A (en) * | 2015-09-07 | 2017-05-24 | 美天施生物科技有限责任公司 | Disposable cartridge for electroporation |
| CN205205150U (en) * | 2015-12-01 | 2016-05-04 | 陈剑 | Electric shock pipe |
| CN205205151U (en) * | 2015-12-01 | 2016-05-04 | 陈剑 | Electric shock pipe |
| CN209098693U (en) * | 2018-09-11 | 2019-07-12 | 苏州壹达生物科技有限公司 | A kind of intermittent streaming electrotransfection device |
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