CN110865116A - Electrochemical nano enzyme sensor for detecting 5-nitroguaiacol sodium - Google Patents
Electrochemical nano enzyme sensor for detecting 5-nitroguaiacol sodium Download PDFInfo
- Publication number
- CN110865116A CN110865116A CN201911169379.8A CN201911169379A CN110865116A CN 110865116 A CN110865116 A CN 110865116A CN 201911169379 A CN201911169379 A CN 201911169379A CN 110865116 A CN110865116 A CN 110865116A
- Authority
- CN
- China
- Prior art keywords
- electrode
- nitroguaiacol
- sodium
- electrochemical
- sensor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/48—Systems using polarography, i.e. measuring changes in current under a slowly-varying voltage
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
-
- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
- C01B32/00—Carbon; Compounds thereof
- C01B32/15—Nano-sized carbon materials
- C01B32/158—Carbon nanotubes
- C01B32/168—After-treatment
-
- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01G—COMPOUNDS CONTAINING METALS NOT COVERED BY SUBCLASSES C01D OR C01F
- C01G39/00—Compounds of molybdenum
- C01G39/06—Sulfides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/308—Electrodes, e.g. test electrodes; Half-cells at least partially made of carbon
-
- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
- C01P2004/00—Particle morphology
- C01P2004/80—Particles consisting of a mixture of two or more inorganic phases
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Nanotechnology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- General Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Inorganic Chemistry (AREA)
- Electrochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Zoology (AREA)
- Pathology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Condensed Matter Physics & Semiconductors (AREA)
- Microbiology (AREA)
- Composite Materials (AREA)
- Genetics & Genomics (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses an electrochemical nano enzyme sensor for detecting 5-nitroguaiacol sodium, which belongs to the technical field of electrochemical sensorsDetecting the content of the 5-nitroguaiacol sodium in the solution to be detected by an ampere method; the composite electrode is molybdenum disulfide (MoS)2) And a carboxylated multi-walled carbon nanotube (COOH-MWCNT). The sensor not only can successfully detect the 5-nitroguaiacol sodium, but also has the characteristics of high sensitivity, strong selectivity, good stability, enzyme property and the like.
Description
Technical Field
The invention belongs to the technical field of electrochemical sensors, and particularly relates to an electrochemical nano enzyme sensor for detecting 5-nitroguaiacol sodium.
Background
The compound sodium nitrophenolate is a plant growth regulator, can promote wound healing and accelerate plant growth, and comprises the chemical components of 5-nitroguaiacol sodium (called 2-methoxy-5-nitrophenolate sodium), o-nitrophenolate sodium and p-nitrophenolate sodium, wherein the 5-nitroguaiacol sodium is the most key component of the compound sodium nitrophenolate, and has the best regulating capability, but the excessive use can cause the risk of teratogenesis. So far, the detection of the 5-nitroguaiacol sodium comprises a high performance liquid chromatography and electrochemical workstation combined technology, a high performance liquid chromatography-normal pressure chemical ionization-mass spectrometry combined technology and a high performance liquid chromatography, but the technologies are time-consuming, high in operation technology, expensive in equipment, complex in sample preparation and not suitable for wide application.
Enzymes play an important role in biocatalysts, but the activity of enzymes is easily destroyed under certain severe conditions, such as inappropriate pH or temperature. With the development of the technology, the nano enzyme becomes a bright point in the field of sensors, has the activity of the enzyme, meets the kinetic equation of the enzyme, and overcomes the severe requirements on catalytic conditions. In addition, nanoenzymes are easy to prepare and have strong durability. Recently, a new generation of materials such as carbon materials, metal nanoparticles, metal oxide nanoparticles, and the like have shown activity of nanoenzymes. These materials have been used for sensing (e.g., detection of ions, molecules, elimination of cells), therapeutic and environmental engineering.
The sensor has the advantages of time saving, high sensitivity, rapidness, simplicity, convenience and high efficiency, the sensor is widely applied to the detection of the aspects of optics, biology, clinic, food, pesticide and the like at present, the electrochemical detection research report on the 5-nitroguaiacol sodium is few at present, and the electrochemical behavior and the determination of the internal wile sensitivity and the like (wile sensitivity, Chenliji, 5-nitroguaiacol on the multiwalled carbon nanotube modified electrode [ J]The 'analysis and test journal' 2010) utilizes a multiwalled carbon nanotube modified electrode to carry out electrochemical detection on 5-nitroguaiacol sodium in a sodium citrate solution, and the linear range is 5 multiplied by 10-5-5×10-4mol L-1The detection limit is 8.6 multiplied by 10-7mol L-1The linear range obtained by the method is narrow, and the sensitivity is low.
Disclosure of Invention
Aiming at the technical problems, the invention provides the electrochemical nano enzyme sensor for detecting the 5-nitroguaiacol sodium, which realizes the electrochemical detection of the 5-nitroguaiacol sodium and has the advantages of simpleness, high efficiency, low cost, rapidness and good selectivity.
In order to achieve the purpose, the invention adopts the following technical scheme:
an electrochemical nanoenzyme sensor for detecting 5-nitroguaiacol sodium comprises a three-electrode system, wherein a saturated calomel electrode is used as a reference electrode, a platinum sheet electrode is used as a counter electrode, a composite electrode is used as a working electrode, acetate buffer solution is used as electrolyte solution, and the content of the 5-nitroguaiacol sodium in a solution to be detected is detected by using a differential pulse voltammetry method; the composite electrode is molybdenum disulfide (MoS)2) And a carboxylated multi-walled carbon nanotube (COOH-MWCNT).
Preferably, the acetate buffer solution has a concentration of 0.1M, a pH of 4, and a voltage window of 0.5-1.0V.
Preferably, the preparation method of the composite electrode is as follows:
(1) weighing single-layer molybdenum disulfide powder, preparing with deionized water, and performing ultrasonic treatment for 30min to obtain 1mg/mL molybdenum disulfide dispersion;
(2) weighing carboxylated multi-walled carbon nanotubes, preparing by using 0.5% perfluorosulfonic acid, and carrying out ultrasonic treatment for 30min to obtain 1mg/mL carboxylated multi-walled carbon nanotube dispersion liquid;
(3) mixing the obtained molybdenum disulfide dispersion liquid and the carboxylated multi-walled carbon nanotube dispersion liquid according to the volume ratio of 1: 1, and performing ultrasonic treatment for 1 hour to obtain the composite material MoS2-COOH-MWCNT;
(4) Polishing glassy carbon electrode, dropping 10 μ L of the above composite material on the glassy carbon electrode, and drying at 60 deg.C to obtain composite electrode MoS2-COOH-MWCNT。
The beneficial technical effects of the invention are as follows: the preparation method of the 5-nitroguaiacol sodium electrochemical nanoenzyme sensor established by the invention has the characteristics of low preparation cost, simple process and simple operation, can successfully detect the 5-nitroguaiacol sodium, has high sensitivity (the lower detection limit can reach 0.03 mu mol/L), strong selectivity, good stability, enzyme property and the like, and can be used for determining the content of the 5-nitroguaiacol sodium in chicken feed and tomatoes.
Drawings
FIG. 1 shows the MoS prepared2-electron micrograph of COOH-MWCNT composite;
FIG. 2 shows the MoS prepared2-differential pulsed voltammogram of COOH-MWCNT/GCE in 20 μmol/L5-nitroguaiacol sodium acetate buffer;
FIG. 3 is a differential pulse voltammogram of prepared composite electrodes in acetate buffer solutions of sodium 5-nitroguaiacol at different concentrations;
FIG. 4 is a graph of current versus concentration for prepared composite electrodes in acetate buffer solutions of sodium 5-nitroguaiacol at various concentrations;
FIG. 5 is a graph of the linear relationship between the reciprocal current and the reciprocal concentration of a prepared composite electrode in acetate buffer solutions of sodium 5-nitroguaiacol at different concentrations;
FIG. 6 is a graph of the stability of the electrochemical nanoenzyme sensor of sodium nitroguaiacol.
Detailed Description
The present invention will be further described with reference to the following specific examples.
Example 1
An electrochemical nanoenzyme sensor for detecting 5-nitroguaiacol sodium comprises a three-electrode system, wherein a saturated calomel electrode is used as a reference electrode, a platinum sheet electrode is used as a counter electrode, a composite electrode is used as a working electrode, acetate buffer solution is used as electrolyte solution, and the content of the 5-nitroguaiacol sodium in a solution to be detected is detected by using a differential pulse voltammetry method; the composite electrode is molybdenum disulfide (MoS)2) And a carboxylated multi-walled carbon nanotube (COOH-MWCNT).
The concentration of acetate buffer solution is 0.1M, pH is 4, and the voltage window is 0.5-1.0V.
The preparation method of the composite electrode comprises the following steps:
(1) weighing single-layer molybdenum disulfide powder, preparing with deionized water, and performing ultrasonic treatment for 30min to obtain 1mg/mL molybdenum disulfide dispersion;
(2) weighing carboxylated multi-walled carbon nanotubes, preparing by using 0.5% perfluorosulfonic acid, and carrying out ultrasonic treatment for 30min to obtain 1mg/mL carboxylated multi-walled carbon nanotube dispersion liquid;
(3) mixing the obtained molybdenum disulfide dispersion liquid and the carboxylated multi-walled carbon nanotube dispersion liquid according to the volume ratio of 1: 1, and performing ultrasonic treatment for 1 hour to obtain the composite material MoS2-COOH-MWCNT;
(4) Polishing glassy carbon electrode, dropping 10 μ L of the above composite material on the glassy carbon electrode, and drying at 60 deg.C to obtain composite electrode MoS2-COOH-MWCNT。
MoS obtained in example 12-COOH-MWCNT composite for appearance characterization, MoS2The morphology of the-COOH-MWCNT composite material is shown in an electron microscope picture of figure 1, the COOH-MWCNT is well dispersed in a perfluorosulfonic acid solution to obtain a uniform dispersion liquid, MoS2The sheet structure is presented, and the composite material shows the appearance of both materials.
The performance of the electrochemical nano enzyme sensor of the 5-nitroguaiacol sodium obtained in the example 1 is detected:
electrochemical behavior of sodium 5-nitroguaiacol: the electrochemical behavior of sodium 5-nitroguaiacol is shown in FIG. 2: in a 0.1mol/L acetate buffer solution containing 20 mu mol/L5-nitroguaiacol sodium, a peak is arranged near 0.8V, a composite electrode has a peak near 0.73V, and the peak current is about 6.5 times of that of a bare electrode, which shows that the composite electrode has good electrocatalytic effect on the 5-nitroguaiacol sodium.
Electrochemical response of sodium 5-nitroguaiacol: the 5-nitroguaiacol sodium with different concentrations is added into the acetate buffer solution with the concentration of 0.1mol/L in sequence, and as can be seen by combining the graph in figure 3 and figure 4, the current is gradually increased between the concentration of 0.09 and 70 mu mol/L, the current is almost saturated and does not change at 70 mu mol/L, the characteristic is similar to the characteristic of the enzyme, and according to the characteristic, the relationship between the reciprocal current and the reciprocal concentration is further studied, the reciprocal current and the reciprocal concentration are found to show a good linear relationship, as shown in figure 5, the Mie's constant of the enzyme can be calculated to be 16.02mM by using the Mie's equation of the enzyme, and the characteristic shows that the composite material has relatively high affinity. In addition, the linearity of the detection of 5-nitroguaiacol sodium is 0.09-70 mu mol/L, and the detection limit is 0.03 mu mol/L.
Reproducibility of detection of 5-nitroguaiacol sodium: 20 mu mol/L of 5-nitroguaiacol sodium is added into 0.1mol/L acetate buffer solution, six different composite electrodes are used for detection in the buffer solution, and as shown in figure 6, the obtained current relative standard deviation RSD value is 0.4839%, which indicates that the repeatability of the composite electrode is good.
In conclusion, the electrochemical nano enzyme sensor for detecting the 5-nitroguaiacol sodium has the characteristics of good repeatability, wide linear range, low detection limit and high affinity of nano enzyme, and in addition, the sensor is a nano enzyme sensor, so that a foundation is provided for application in other fields.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and various modifications and variations which do not require inventive efforts and are made by those skilled in the art are still within the scope of the present invention.
Claims (3)
1. DetectionThe electrochemical nanoenzyme sensor of the 5-nitroguaiacol sodium is characterized in that a three-electrode system is formed by taking a saturated calomel electrode as a reference electrode, a platinum sheet electrode as a counter electrode and a composite electrode as a working electrode, acetate buffer solution is taken as electrolyte solution, and the content of the 5-nitroguaiacol sodium in the solution to be detected is detected by using a differential pulse voltammetry method; the composite electrode is molybdenum disulfide (MoS)2) And a carboxylated multi-walled carbon nanotube (COOH-MWCNT).
2. The electrochemical nanoenzyme sensor for detecting sodium 5-nitroguaiacol as claimed in claim 1, wherein the concentration of acetate buffer solution is 0.1M, pH is 4, and the voltage window is 0.5-1.0V.
3. The electrochemical nanoenzyme sensor for detecting sodium 5-nitroguaiacol as claimed in claim 1, wherein the composite electrode is prepared by the following steps:
(1) weighing single-layer molybdenum disulfide powder, preparing with deionized water, and performing ultrasonic treatment for 30min to obtain 1mg/mL molybdenum disulfide dispersion;
(2) weighing carboxylated multi-walled carbon nanotubes, preparing by using 0.5% perfluorosulfonic acid, and carrying out ultrasonic treatment for 30min to obtain 1mg/mL carboxylated multi-walled carbon nanotube dispersion liquid;
(3) mixing the obtained molybdenum disulfide dispersion liquid and the carboxylated multi-walled carbon nanotube dispersion liquid according to the volume ratio of 1: 1, and performing ultrasonic treatment for 1 hour to obtain the composite material MoS2-COOH-MWCNT;
(4) Polishing glassy carbon electrode, dropping 10 μ L of the above composite material on the glassy carbon electrode, and drying at 60 deg.C to obtain composite electrode MoS2-COOH-MWCNT。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201911169379.8A CN110865116A (en) | 2019-11-25 | 2019-11-25 | Electrochemical nano enzyme sensor for detecting 5-nitroguaiacol sodium |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201911169379.8A CN110865116A (en) | 2019-11-25 | 2019-11-25 | Electrochemical nano enzyme sensor for detecting 5-nitroguaiacol sodium |
Publications (1)
Publication Number | Publication Date |
---|---|
CN110865116A true CN110865116A (en) | 2020-03-06 |
Family
ID=69656620
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201911169379.8A Pending CN110865116A (en) | 2019-11-25 | 2019-11-25 | Electrochemical nano enzyme sensor for detecting 5-nitroguaiacol sodium |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN110865116A (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111337558A (en) * | 2020-04-06 | 2020-06-26 | 东莞正大康地饲料有限公司 | Method for measuring sodium content in feed by using ion selective electrode |
CN115165988A (en) * | 2022-03-01 | 2022-10-11 | 浙江大学 | Working electrode for detecting nitrite and detection method thereof |
CN115389601A (en) * | 2022-09-09 | 2022-11-25 | 湘潭大学 | Preparation of luteolin electrochemical sensor and method for detecting luteolin |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101029886A (en) * | 2007-03-29 | 2007-09-05 | 上海大学 | Method for determining nitrobenzyl phenol three isomer simultaneouslly |
CN103558271A (en) * | 2013-10-17 | 2014-02-05 | 宁波大学 | Electrochemical biosensor for detecting penicillin and preparation method and application thereof |
CN106770548A (en) * | 2016-12-07 | 2017-05-31 | 扬州大学 | Molybdenum sulfide multi-walled carbon nano-tubes gold modified glassy carbon electrode |
-
2019
- 2019-11-25 CN CN201911169379.8A patent/CN110865116A/en active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101029886A (en) * | 2007-03-29 | 2007-09-05 | 上海大学 | Method for determining nitrobenzyl phenol three isomer simultaneouslly |
CN103558271A (en) * | 2013-10-17 | 2014-02-05 | 宁波大学 | Electrochemical biosensor for detecting penicillin and preparation method and application thereof |
CN106770548A (en) * | 2016-12-07 | 2017-05-31 | 扬州大学 | Molybdenum sulfide multi-walled carbon nano-tubes gold modified glassy carbon electrode |
Non-Patent Citations (2)
Title |
---|
YUPING LIN ET.AL: "Non-enzymatic sensing of hydrogen peroxide using a glassy carbon electrode modified with a nanocomposite made from carbon nanotubes and molybdenum disulfide", 《MICROCHIM ACTA》 * |
任健敏 等: "5-硝基愈创木酚在多壁碳纳米管修饰电极上的电化学行为及其测定", 《分析测试学报》 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111337558A (en) * | 2020-04-06 | 2020-06-26 | 东莞正大康地饲料有限公司 | Method for measuring sodium content in feed by using ion selective electrode |
CN111337558B (en) * | 2020-04-06 | 2023-03-28 | 正大康地农牧集团有限公司 | Method for measuring sodium content in feed by using ion selective electrode |
CN115165988A (en) * | 2022-03-01 | 2022-10-11 | 浙江大学 | Working electrode for detecting nitrite and detection method thereof |
CN115389601A (en) * | 2022-09-09 | 2022-11-25 | 湘潭大学 | Preparation of luteolin electrochemical sensor and method for detecting luteolin |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Jiang et al. | Electrodeposition of TiO2 nanoparticles on multiwalled carbon nanotube arrays for hydrogen peroxide sensing | |
Matsunaga et al. | Detection of microbial cells by cyclic voltammetry | |
Wei et al. | Voltammetric determination of folic acid with a multi-walled carbon nanotube-modified gold electrode | |
Liu et al. | Highly sensitive and selective electrochemical detection of L-cysteine using nanoporous gold | |
Zhang et al. | Electrochemical detection of hydrazine based on electrospun palladium nanoparticle/carbon nanofibers | |
Wu et al. | Electrochemical study and selective determination of dopamine at a multi-wall carbon nanotube-nafion film coated glassy carbon electrode | |
Liu et al. | Electrocatalysis via direct electrochemistry of myoglobin immobilized on colloidal gold nanoparticles | |
Wang et al. | Application of a single-wall carbon nano-tube film electrode to the determination of trace amounts of folic acid | |
CN110865116A (en) | Electrochemical nano enzyme sensor for detecting 5-nitroguaiacol sodium | |
Chen et al. | Acetylcholinesterase biosensor for chlorpyrifos detection based on multi-walled carbon nanotubes-SnO2-chitosan nanocomposite modified screen-printed electrode | |
Zhu et al. | Ordered mesoporous carbon paste electrodes for electrochemical sensing and biosensing | |
CN105954336B (en) | A kind of no enzyme superoxide anion electrochemical sensor and its preparation method and application | |
Figueiredo-Filho et al. | Electroanalytical determination of the linuron herbicide using a cathodically pretreated boron-doped diamond electrode: comparison with a boron-doped diamond electrode modified with platinum nanoparticles | |
Mazloum-Ardakani et al. | Carbon nanoparticles and a new derivative of hydroquinone for modification of a carbon paste electrode for simultaneous determination of epinephrine and acetaminophen | |
Ganjali et al. | Determination of Salicylic Acid by Differential Pulse Voltammetry Using ZnO/Al2O3 Nanocomposite Modified Graphie Screen Printed Electrode | |
CN105044171A (en) | Production method and application of nano-platinum doped/enzyme modified carbon paste electrode | |
CN109100408A (en) | It is a kind of based on iron-phthalocyanine/nitrogen, the electrochemical sensor of boron doping redox graphene and its preparation method and application | |
Chen et al. | A simple acetylcholinesterase biosensor based on ionic liquid/multiwalled carbon nanotubes-modified screen-printed electrode for rapid detecting chlorpyrifos | |
Sun et al. | Direct Electrochemistry of Myoglobin in a Nafion‐Ionic Liquid Composite Film Modified Carbon Ionic Liquid Electrode | |
Dong et al. | Enhanced amperometric sensing for direct detection of nitenpyram via synergistic effect of copper nanoparticles and nitrogen-doped graphene | |
Shahrokhian et al. | Electrocatalytic Oxidation of 2‐Thiouracil and 2‐Thiobarbituric Acid at a Carbon‐Paste Electrode Modified with Cobalt Phthalocyanine | |
PA et al. | A sensitive novel approach towards the detection of 8-hydroxyquinoline at anionic surfactant modified carbon nanotube based biosensor: A voltammetric study | |
Hu et al. | Simultaneous determination of dopamine and ascorbic acid using the nano‐gold self‐assembled glassy carbon electrode | |
Niu et al. | Electrochemical behavior of epinephrine at a penicillamine self-assembled gold electrode, and its analytical application | |
CN108918623A (en) | A kind of preparation method and application of the Electrochemical enzyme biosensor based on zinc-base metal-organic framework materials and nanogold composite material |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200306 |
|
RJ01 | Rejection of invention patent application after publication |