CN110823855B - Method for rapidly detecting amphetamine drugs in human urine - Google Patents
Method for rapidly detecting amphetamine drugs in human urine Download PDFInfo
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- CN110823855B CN110823855B CN201911315924.XA CN201911315924A CN110823855B CN 110823855 B CN110823855 B CN 110823855B CN 201911315924 A CN201911315924 A CN 201911315924A CN 110823855 B CN110823855 B CN 110823855B
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- Y02P20/00—Technologies relating to chemical industry
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Abstract
The invention discloses a method for rapidly detecting amphetamine drugs in human urine, which utilizes hydrophobic ionic liquid to selectively adsorb the amphetamine drugs, and generates fluorescent substances through rapid reaction of sulfonyl chloride probe substances and the amphetamine drugs so as to judge whether the urine contains the amphetamine drugs, wherein the amphetamine drugs comprise: amphetamine, methamphetamine, methylenedioxyamphetamine, methylenedioxymethamphetamine, and the like. The invention fully utilizes the selective adsorption effect of the hydrophobic ionic liquid on amphetamine drugs in urine to achieve the purpose of enrichment, thereby realizing the effect of high detection limit of the method. The sulfonyl chloride probe substance and amphetamine drugs are utilized to react rapidly in the hydrophobic ionic liquid to generate fluorescent substances, so that the method has the characteristics of high accuracy, rapid and quick detection, simple and easy operation and easy grasp.
Description
Technical Field
The invention relates to the field of drug detection, in particular to a method for rapidly detecting amphetamine drugs in human urine.
Background
Inhalation and injection of drugs has become a worldwide nuisance, and drugs are known as sources of crimes that spread white plague. In recent decades, the number of people who take medicine worldwide is continuously growing and spreading, and various unstable factors brought to society due to medicine taking, the harm to social security, the epidemic and transmission of infectious diseases caused by the medicine taking, the epidemic of venereal diseases and AIDS viruses, and the way of getting deep pain and absolute has been reached.
By using a proper analysis and detection means, relevant personnel are checked, drug addicts are accurately identified, and the method is very important for strengthening social security, developing drug administration, timely giving up drug addicts and carrying out tracking investigation on the drug addicts, realizing community correction aid measures, improving withdrawal consolidation rate and reducing relapse rate. However, the existing method for detecting and identifying the drug addict uses gas chromatograph, gas-mass spectrometer and other devices. The device is high in price, complex in operation and high in detection cost, and is difficult to realize on-site rapid detection, so that the requirement of actual work cannot be met.
Ionic liquids refer to salts composed of anions and cations, also known as low temperature molten salts, that are in a liquid state at or near room temperature. Ionic liquids have received increasing attention from workers in various disciplines because of their inherent incomparable advantages over many conventional solvents. One of the most important characteristics of ionic liquids is the selective dissolution capability. Currently, researchers have utilized this feature to separate and enrich a variety of substances using different methods.
Disclosure of Invention
In order to solve the problems, the invention provides a method for rapidly detecting amphetamine drugs in human urine.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
a method for rapidly detecting amphetamine-type drugs in human urine utilizes hydrophobic ionic liquid added with sulfonyl chloride probe substances to detect the amphetamine-type drugs in the urine.
Further, the selective adsorption of the amphetamine drugs by the hydrophobic ionic liquid is utilized, and fluorescent substances are generated by the rapid reaction of sulfonyl chloride probe substances and the amphetamine drugs, so that whether the urine contains the amphetamine drugs is judged.
Further, the drugs of the phenylpropanoids include: amphetamine, methamphetamine, methylenedioxyamphetamine, methylenedioxymethamphetamine, and the like.
Further, the hydrophobic ionic liquid is N-alkyl pyridine ionic liquid, alkyl imidazole ionic liquid, functionalized imidazole ionic liquid, quaternary ammonium salt ionic liquid, pyrrolidine ionic liquid or piperidine ionic liquid.
Further, the method comprises the following steps:
s1, uniformly mixing dichloromethane, hydrophobic ionic liquid and sulfonyl chloride reagent in a mass ratio of 500:100:1, preparing test paper or test board and the like, and airing;
s2, collecting a urine sample of the tested person, immersing test paper or a test board and the like in the urine sample for 3-5 min, and under the irradiation of ultraviolet light, if fluorescence is generated, the tested person is proved to drink or inject amphetamine drugs, otherwise, the tested person is proved to not drink or inject amphetamine drugs.
The invention has the following beneficial effects:
1. the selective adsorption effect of the hydrophobic ionic liquid on amphetamine drugs in urine is fully utilized, the purpose of enrichment is achieved, and therefore the effect of high detection limit of the method is achieved.
2. The sulfonyl chloride probe substance and amphetamine drugs are utilized to react rapidly in the hydrophobic ionic liquid to generate fluorescent substances, so that the method has the characteristics of high accuracy, rapid and quick detection, simple and easy operation and easy grasp.
3. The method can be used for manufacturing test paper, test board and the like which are convenient for field use through the processes of coating, dipping, carrying, depositing, grafting and the like, can judge whether a tested person absorbs the toxin on site, and has strong practicability.
Drawings
FIG. 1 shows the chemical structure of amphetamine-type drugs according to an embodiment of the present invention.
FIG. 2 shows the chemical structural formula of sulfonyl chloride-based reagent in the examples of the present invention.
Detailed Description
The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the present invention, but are not intended to limit the invention in any way. It should be noted that variations and modifications could be made by those skilled in the art without departing from the inventive concept. These are all within the scope of the present invention.
The embodiment of the invention provides a method for rapidly detecting amphetamine drugs in human urine, which utilizes hydrophobic ionic liquid to selectively adsorb the amphetamine drugs, and generates fluorescent substances through rapid reaction of sulfonyl chloride probe substances and the amphetamine drugs so as to judge whether the urine contains the amphetamine drugs. The drugs of the phenylpropanamine comprise: amphetamine, methamphetamine, methylenedioxyamphetamine, methylenedioxymethamphetamine, etc., and the chemical structural formula is shown in figure 1. The chemical structural formula in figure 1 can find that the amphetamine drugs have the common characteristic that primary amine or secondary amine groups are contained in the structure, and the method provides opportunities for finding a chemical analysis method with high accuracy, rapid detection and simple and easy operation.
The aim of the invention is to selectively adsorb amphetamine drugs in urine with high efficiency by using hydrophobic ionic liquid, and the specific ionic liquid comprises (as shown in table 1):
table 1: hydrophobic ionic liquid species
The sulfonyl chloride probe substance disclosed by the invention refers to a sulfonyl chloride reagent capable of rapidly reacting with amphetamine drugs in a hydrophobic ionic liquid and generating fluorescent compounds, and specifically comprises, but is not limited to: the chemical structural formulas of the 5- (diethylamino) naphthalene-1-sulfonyl chloride, the 5- (dimethylamino) naphthalene-2-sulfonyl chloride, the 6- (methylamino) naphthalene-2-sulfonyl chloride, the 5-ethoxynaphthalene-1-sulfonyl chloride, the 6-methoxynaphthalene-2-sulfonyl chloride, the anthracene-2-sulfonyl chloride and the 4-methoxybenzene-1-sulfonyl chloride are shown in figure 2.
The implementation method of the invention comprises the following steps:
the methylene dichloride, the hydrophobic ionic liquid and the sulfonyl chloride reagent are uniformly mixed according to the mass ratio of 500:100:1, and then are manufactured into test paper or test board through the processes of coating, dipping, carrying, depositing, grafting and the like, and the test paper or test board can be used after being dried in the air. After a urine sample of a tested person is collected, a test paper, a test board and the like are placed in the sample, and after the sample paper, the test board and the like are immersed for 3-5 min, under the irradiation of ultraviolet light, if fluorescence is generated, the tested person is proved to absorb or inject amphetamine drugs, otherwise, the tested person is proved to not absorb or inject amphetamine drugs.
Verification test of anti-drug interference ability:
after adding 1mg of the common drug or standard to 100mL of blank urine, respectively, the results were measured by the method described above and are shown in table 2 below:
TABLE 2
The foregoing describes specific embodiments of the present invention. It is to be understood that the invention is not limited to the particular embodiments described above, and that various changes or modifications may be made by those skilled in the art within the scope of the appended claims without affecting the spirit of the invention. The embodiments of the present application and features in the embodiments may be combined with each other arbitrarily without conflict.
Claims (2)
1. A method for rapidly detecting amphetamine drugs in human urine is characterized by comprising the following steps: selectively adsorbing amphetamine drugs in urine by utilizing hydrophobic ionic liquid added with sulfonyl chloride probe substances, and rapidly reacting the sulfonyl chloride probe substances with the amphetamine drugs to generate fluorescent substances so as to judge whether the urine contains the amphetamine drugs;
the drugs of the phenylpropanamine comprise: amphetamine, methamphetamine, methylenedioxyamphetamine and methylenedioxymethamphetamine;
the hydrophobic ionic liquid is N-alkyl pyridine ionic liquid, functionalized imidazole ionic liquid, quaternary ammonium salt ionic liquid, pyrrolidine ionic liquid or piperidine ionic liquid;
the method comprises the following steps:
s1, uniformly mixing methylene dichloride/hydrophobic ionic liquid/sulfonyl chloride reagent in a mass ratio of 500:100:1, preparing test paper or test board, and airing;
s2, collecting a urine sample of the tested person, immersing test paper or a test board in the urine sample for 3-5 min, and under the irradiation of ultraviolet light, if fluorescence is generated, the tested person is proved to absorb or inject amphetamine drugs, otherwise, the tested person is proved to not absorb or inject amphetamine drugs.
2. The method for rapidly detecting amphetamine-type drugs in human urine according to claim 1, wherein the method comprises the following steps:
the functional imidazole ionic liquid is alkyl imidazole ionic liquid.
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| CN102043052A (en) * | 2009-10-13 | 2011-05-04 | 陈洁 | Rapid detection kit for two items of joint inspection of drug |
| WO2016010855A1 (en) * | 2014-07-15 | 2016-01-21 | C2Sense Llc | Formulations for enhanced chemiresistive sensing |
| CN105572241B (en) * | 2014-11-05 | 2018-01-19 | 复旦大学 | The method for determining amphetamine in blood and urine, ketamine, pethidine and methadone |
| CN104927834B (en) * | 2015-05-11 | 2017-03-01 | 池州学院 | A kind of disubstituted polyacetylene fluorescent dye of carbazolyl-containing group and preparation method thereof |
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| 唐英章 等.无.《保健食品中功效成分及危害物质的分析》.中国标准出版社,2004, * |
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