CN110819561B - Actinomycete TL-007 and application thereof - Google Patents
Actinomycete TL-007 and application thereof Download PDFInfo
- Publication number
- CN110819561B CN110819561B CN201911089241.7A CN201911089241A CN110819561B CN 110819561 B CN110819561 B CN 110819561B CN 201911089241 A CN201911089241 A CN 201911089241A CN 110819561 B CN110819561 B CN 110819561B
- Authority
- CN
- China
- Prior art keywords
- actinomycete
- ginseng
- application
- strain
- streptomyces
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/465—Streptomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
An actinomycete TL-007 and application thereof, relating to an actinomycete TL-007 and application thereof. The invention aims to provide an actinomycete TL-007 with broad-spectrum antagonistic action and application thereof. The actinomycete TL-007 is streptomycete nigricans (Streptomyces niger) TL-007 which is preserved in the common microorganism center of China Committee for culture Collection of microorganisms, the preservation address is No. 3 Siro No.1 of Beijing Korean district, the preservation date is 2019, 9 and 29 days, and the preservation number is CGMCC No. 18633. The application of the actinomycete TL-007 is the application in crop disease control. The TL-007 has the bacteriostasis rate of 85.93 percent on rust rot, is a bacterial strain for efficiently antagonizing ginseng rust rot and has broad-spectrum antagonistic action. The invention is applied to the technical field of environmental microorganisms.
Description
Technical Field
The invention relates to an actinomycete TL-007 and application thereof.
Background
In recent years, the chemical method for preventing and treating crop diseases has the characteristics of short time and quick response, but has the serious problem of chemical pesticide residue in the prevention and treatment process, and the problems of environmental pollution and drug resistance of germs caused by excessive use of chemical pesticides. For example, ginseng is one of the famous and precious Chinese traditional medicinal materials for preventing and treating ginseng rust rot, but various diseases often occur in the cultivation process to influence the yield and quality of ginseng. The ginseng rust rot is a soil-borne disease of ginseng roots, and chlamydospores of the ginseng rust rot can survive in soil for a long time in a dormant mode under a host-free condition to cause soil bacteria, so that the planting of the next batch of ginseng is directly influenced, and the ginseng is prevented from continuous cropping. The chemical method is adopted for preventing and treating the ginseng root from accumulating a large amount of toxic substances, which seriously affects the quality of the ginseng; the traditional agricultural control adopts measures of applying organic fertilizer, reasonably rotating crop, changing autumn planting into spring planting, changing old ginseng land into farmland and the like, but most of the measures can only play a role in prevention. Therefore, biological control is one of the main approaches for controlling crop diseases, and a biocontrol bacterium with broad-spectrum antagonism is urgently needed.
Disclosure of Invention
The invention aims to provide an actinomycete TL-007 with broad-spectrum antagonistic action and application thereof.
The actinomycete TL-007 is streptomycete nigricans (Streptomyces niger) TL-007 which is preserved in the common microorganism center of China Committee for culture Collection of microorganisms, the preservation address is No. 3 Siro No.1 of Beijing Korean district, the preservation date is 2019, 9 and 29 days, and the preservation number is CGMCC No. 18633.
The application of the actinomycete TL-007 is the application in crop disease control.
The invention adopts a plate dilution method to separate and obtain 54 ginseng rhizosphere actinomycetes from mountain city in Jilin province, and carries out screening of antagonistic rust rot.
And (3) separation and purification of actinomycetes: taking 1.0g of a soil sample, air-drying, grinding and dispersing uniformly, and mixing the materials in a proportion of 1: dissolving in 10% sterile water, filtering to obtain filtrate 10-1Starting solution, successively obtaining 10-2And 10-3And (5) soil gradient dilution. Respectively diluting and coating on Gao's 1 and PDA isolated culture medium, culturing at 28 deg.C for 7d until single colony grows out, selecting single colony, and purifying on culture medium to obtain single strain.
Screening antagonistic actinomycetes: the method comprises inoculating rust rot bacteria on PDA culture medium for activation by plate confrontation. On a sterile operating platform, a 1cm puncher is used for taking a rusty rot fungi cake to be placed in the center of a PDA flat plate, antagonistic bacteria hyphae are taken to draw a straight line at a position about 2cm away from pathogenic bacteria, and the flat plate without antagonistic bacteria hyphae is used as a contrast. Each set of 3 replicates was incubated in a 28 ℃ incubator and colony growth was observed and the minimum radius of the bacteria and the radius of the control colony were recorded at 5 d. The calculation formula ═ (control colony radius-pathogen colony radius)/control colony radius × 100%. The bacteriostatic rate of TL-007 to rust rot is 85.93%.
ITS sequence and phylogenetic analysis of actinomycete TL-007: the obtained 16S rDNA gene sequence of the strain is compared with known sequences in a GenBank database by BLAST, and the MEGA 5.1 is adopted to construct a phylogenetic tree TL-007 strain which belongs to the same branch of Streptomyces nogalaters, and the similarity of the strain reaches 100 percent. Finally named as Streptomyces nigricans (Streptomyces nogalaters) TL-007 through morphological characteristics and molecular biological identification results.
The invention discloses a ginseng rhizosphere actinomycete TL-007 strain with the preservation number of CGMCC NO. 18633. Among a large amount of ginseng rhizosphere microorganisms, a plurality of strains capable of antagonizing ginseng rust rot are obtained through primary screening, and then the strains with the best antagonistic effect are obtained through repeated screening, wherein the bacteriostatic rate of TL-007 to the rust rot is 85.93%. Through identification, the strain TL-007 is black walnut Streptomyces (Streptomyces nogalaters), and the high-efficiency antagonistic strain can effectively inhibit rust rot of ginseng and is free of pathogenicity. Lays a good foundation for the development and the utilization of bacterial manure for preventing and treating ginseng rust rot. The bacterial strain is subjected to an antibacterial spectrum experiment, and the experiment shows that the antibacterial rates of TL-007 on soybean black spot, soybean gray mold, Chinese cabbage black rot and ginseng fusarium are respectively as follows: 80.37 percent, 76.37 percent, 81.48 percent and 82.66 percent, which shows that the compound has antagonism effect on diseases of crops such as soybean, Chinese cabbage, ginseng and the like and has broad-spectrum antagonism effect. The strain TL-007 is a strain for efficiently antagonizing ginseng rust rot and has a broad-spectrum antagonistic effect, and the biological control means, namely, the organisms or metabolites thereof are utilized to effectively control plant diseases, so that compared with other traditional chemical control means, the strain TL-007 is more environment-friendly, has no pesticide residue and does not generate drug resistance.
Drawings
FIG. 1 is the colony morphology of TL-007 strain on PDA medium;
FIG. 2 is a microscopic picture of TL-007 strain;
FIG. 3 is a graph of antagonism of the TL-007 strain to ginseng rust rot;
FIG. 4 is the bacterial inhibition spectrum of TL-007 strain;
FIG. 5 is a molecular identification map of TL-007 strain;
FIG. 6 is a preliminary back-grafting validation of TL-007 strain on ginseng sections.
Detailed Description
The first embodiment is as follows: the actinomycete TL-007 of the embodiment is streptomycete nigricans (Streptomyces niger) TL-007 which is preserved in the common microorganism center of China Committee for culture Collection of microorganisms, the preservation address is No. 3 Siro No.1 of Beijing Kogyo area north Chen, the preservation date is 2019, 29 days 9 and 18633, and the preservation number is CGMCC No. 18633.
In the embodiment, 54 ginseng rhizosphere actinomycetes from mountain city, Jilin province and 54 ginseng rhizosphere actinomycetes are obtained by adopting a flat plate dilution method, and are screened for resisting rust rot.
And (3) separation and purification of actinomycetes: taking 1.0g of a soil sample, air-drying, grinding and dispersing uniformly, and mixing the materials in a proportion of 1: dissolving in 10% sterile water, filtering to obtain filtrate 10-1Starting solution, successively obtaining 10-2And 10-3And (5) soil gradient dilution. Respectively diluting and coating on Gao's 1 and PDA isolated culture medium, culturing at 28 deg.C for 7d until single colony grows out, selecting single colony, and purifying on culture medium to obtain single strain.
Screening antagonistic actinomycetes: the method comprises inoculating rust rot bacteria on PDA culture medium for activation by plate confrontation. On a sterile operating platform, a 1cm puncher is used for taking a rusty rot fungi cake to be placed in the center of a PDA flat plate, antagonistic bacteria hyphae are taken to draw a straight line at a position about 2cm away from pathogenic bacteria, and the flat plate without antagonistic bacteria hyphae is used as a contrast. Each set of 3 replicates was incubated in a 28 ℃ incubator and colony growth was observed and the minimum radius of the bacteria and the radius of the control colony were recorded at 5 d. The calculation formula ═ (control colony radius-pathogen colony radius)/control colony radius × 100%. The bacteriostatic rate of TL-007 to rust rot is 85.93%.
ITS sequence and phylogenetic analysis of actinomycete TL-007: the obtained 16S rDNA gene sequence of the strain is compared with known sequences in a GenBank database by BLAST, and the MEGA 5.1 is adopted to construct a phylogenetic tree TL-007 strain which belongs to the same branch of Streptomyces nogalaters, and the similarity of the strain reaches 100 percent. Finally named as Streptomyces nigricans (Streptomyces nogalaters) TL-007 through morphological characteristics and molecular biological identification results.
The embodiment utilizes a biological control means, namely, utilizes organisms or metabolites thereof to effectively control plant diseases, is more environment-friendly compared with other traditional chemical control, does not generate drug resistance, and provides a theoretical basis for the development and effective utilization of microorganism resources for control. Thereby laying a long-term development for green organic agriculture.
The second embodiment is as follows: the application of the actinomycete TL-007 in the embodiment is the application in crop disease control.
The strain TL-007 of the embodiment is the Streptomyces nigricans (Streptomyces niger), and the efficient antagonistic strain can effectively inhibit the rust rot of ginseng and is free of pathogenicity. Lays a good foundation for the development and the utilization of bacterial manure for preventing and treating ginseng rust rot. The bacterial strain is subjected to an antibacterial spectrum experiment, and the experiment shows that the antibacterial rates of TL-007 on soybean black spot, soybean gray mold, Chinese cabbage black rot and ginseng fusarium are respectively as follows: 80.37 percent, 76.37 percent, 81.48 percent and 82.66 percent, which shows that the compound has antagonism effect on diseases of crops such as soybean, Chinese cabbage, ginseng and the like and has broad-spectrum antagonism effect.
The third concrete implementation mode: the second embodiment is different from the first embodiment in that: the actinomycete TL-007 is applied to the inhibition of soybean black spot pathogenic bacteria. The rest is the same as the second embodiment.
The fourth concrete implementation mode: the second or third embodiment is different from the first or second embodiment in that: the actinomycete TL-007 is applied to the inhibition of the botrytis cinerea. The other embodiments are the same as the second or third embodiment.
The fifth concrete implementation mode: this embodiment is different from one of the second to fourth embodiments in that: the actinomycete TL-007 is applied to the inhibition of Chinese cabbage black rot pathogen. The other is the same as one of the second to fourth embodiments.
The sixth specific implementation mode: the present embodiment is different from one of the second to fifth embodiments in that: the actinomycete TL-007 is applied to the inhibition of ginseng fusarium pathogenic bacteria. The rest is the same as one of the second to fifth embodiments.
The seventh embodiment: the present embodiment is different from one of the second to sixth embodiments in that: the actinomycete TL-007 is applied to inhibiting ginseng rust rot pathogenic bacteria. The rest is the same as one of the second to sixth embodiments.
The specific implementation mode is eight: in the embodiment, 54 ginseng rhizosphere actinomycetes from mountain city, Jilin province and 54 ginseng rhizosphere actinomycetes are obtained by adopting a flat plate dilution method, and are screened for resisting rust rot.
And (3) separation and purification of actinomycetes: taking 1.0g of a soil sample, air-drying, grinding and dispersing uniformly, and mixing the materials in a proportion of 1: dissolving in 10% sterile water, filtering to obtain filtrate 10-1Starting solution, successively obtaining 10-2And 10-3And (5) soil gradient dilution. Respectively performing dilution coating on Gao's 1 and PDA separation culture media, culturing at 28 ℃ for 7d until a single colony grows out, picking the single colony on the culture media, and purifying until single strains (figure 1) and (figure 2) are finally obtained.
Screening antagonistic actinomycetes: the method comprises inoculating rust rot bacteria on PDA culture medium for activation by plate confrontation. On a sterile operating platform, a 1cm puncher is used for taking a rusty rot fungi cake to be placed in the center of a PDA flat plate, antagonistic bacteria hyphae are taken to draw a straight line at a position about 2cm away from pathogenic bacteria, and the flat plate without antagonistic bacteria hyphae is used as a contrast. Each set of 3 replicates was incubated in a 28 ℃ incubator and colony growth was observed and the minimum radius of the bacteria and the radius of the control colony were recorded at 5d (FIG. 3). The calculation formula ═ (control colony radius-pathogen colony radius)/control colony radius × 100%. The bacteriostatic rate of TL-007 to rust rot is 85.93%.
The fifth concrete implementation mode: identification of Streptomyces sp.g6 with broad-spectrum antagonistic effect of the present embodiment:
1. physiological and biochemical identification
The physiological and biochemical characteristics of the TL-007 strain are measured by referring to the culture medium and the method recommended in the handbook of identifying common bacteria system and the handbook of identifying Bergey system, and the tolerance of the strain for liquefying gelatin, producing catalase, starch hydrolase, producing indoleacetic acid and NaCl is measured to be 7 percent (Table 1).
TABLE 1 Actinomycetes TL-007 physiological and biochemical test
Note: the physiological and biochemical test results of actinomycete TL-007: "+" is positive; "-" is negative
2. ITS sequence of actinomycete TL-007 and phylogenetic analysis
Obtaining a 16S rDNA gene sequence of the strain, wherein the DNA sequence is shown as SEQ ID NO: 1, then performing BLAST comparison analysis with known sequences in a GenBank database, and adopting MEGA 5.1 to construct a phylogenetic tree TL-007 strain, which belongs to the same branch as a Streptomyces nogalaters, wherein the similarity reaches 100%. As a result of morphological characteristics and molecular biological identification, the TL-007 strain was a Streptomyces nigricans strain (Streptomyces nogalaters) (FIG. 5).
The Streptomyces sp.G6 of the embodiment has the following functional verification on crop disease control:
experiment 1, antagonism of soybean black spot, soybean gray mold, cabbage black rot, ginseng fusarium by plate confrontation method
Calculating the bacteriostatic rate of TL-007 to soybean black spot, soybean gray mold, cabbage black rot and ginseng fusarium (figure 4) by adopting a plate confronting method and referring to a method for screening antagonistic actinomycetes (respectively): 80.37%, 76.37%, 81.48%, 82.66%. The product has antagonistic effect on diseases of soybean, Chinese cabbage, ginseng and other crops, and has broad-spectrum antagonistic effect.
Test 2: primary back grafting verification of TL-007 strain on ginseng slice
Culturing the obtained TL-007 bacteria in a Gao's I liquid at 28 ℃ for 3d, centrifuging at 10000rpm to obtain bacteria, adding water, blowing, centrifuging again for 1 time, and cleaning the bacteria. The obtained cells were prepared into 10 with sterile water8cfu/mL. Placing Ginseng radix stem block in sterilized culture dish, processing in 4 groups without any CK group, inoculating rusty corrosion bacteria on Ginseng radix stem block, adding Ginseng radix biocontrol bacteria suspension, and processing with Ginseng radix biocontrol bacteria suspension alone. The vessel was placed in an incubator at 28 ℃ for 5 days, and then the ginseng stem pieces were observed for decay (FIG. 6). The results show that: the growth of the rust rot fungi can be obviously inhibited by adding the antagonistic bacteria TL-007, and the fungi mass of the antagonistic bacteria TL-007 which is added independently has no rot, which indicates that the antagonistic bacteria TL-007 has no damage to the ginseng stems and the antagonistic bacteria TL-007 has the biological control effect.
Sequence listing
<110> institute of geography and agroecology of northeast China academy of sciences
<120> actinomycete TL-007 and application thereof
<160> 1
<210> 1
<211> 990
<212> DNA
<213> Streptomyces nigricans strain (Streptomyces nogalaters)
<400> 1
agggcgtgcg ggcgtgctta cacatgcaag tcgacgatga acctccttcg ggaggggatt 60
agtggcgaac gggtgagtaa cacgtgggca atctgccctg cactctggga caagccctgg 120
aaacggggtc taataccgga tacgagcctc caccgcatgg tgggggttgg aaagctccgg 180
cggtgcagga tgagcccgcg gcctatcagc ttgttggtga ggtaacggct caccaaggcg 240
acgacgggta gccggcctga gagggcgacc ggccacactg ggactgagac acggcccaga 300
ctcctacggg aggcagcagt ggggaatatt gcacaatggg cgaaagcctg atgcagcgac 360
gccgcgtgag ggatgacggc cttcgggttg taaacctctt tcagcaggga agaagcgaga 420
gtgacggtac ctgcagaaga agcgccggct aactacgtgc cagcagccgc ggtaatacgt 480
agggcgcaag cgttgtccgg aattattggg cgtaaagagc tcgtaggcgg cttgtcacgt 540
cggttgtgaa agcccggggc ttaaccccgg gtctgcagtc gatacgggca ggctagagtt 600
cggtagggga gatcggaatt cctggtgtag cggtgaaatg cgcagatatc aggaggaaca 660
ccggtggcga aggcggatct ctgggccgat actgacgctg aggagcgaaa gcgtggggag 720
cgaacaggat tagataccct ggtagtccac gccgtaaacg gtgggcacta ggtgtgggca 780
acattccacg ttgtccgtgc cgcagctaac gcattaagtg ccccgcctgg ggagtacggc 840
cgcaaggcta aaactcaaag gaattgacgg gggcccgcac aagcggcgga gcatgtggct 900
taattcgacg caacgcgaag accttaccaa ggcttgacat acaccggaaa gcattagaga 960
tagtgccccc cttgtggtcg gtgtacaggt 990
Claims (2)
1. An actinomycete TL-007 which is characterized in that the actinomycete TL-007 is Streptomyces nigricans (Streptomyces niger) TL-007 which is preserved in the common microorganism center of China Committee for culture Collection of microorganisms, the preservation address is No. 3 Siro No.1 Hospital No. 3 of Beijing, Chaoyang, the preservation date is 2019, 29 days, and the preservation number is CGMCC No. 18633.
2. The use of an actinomycete TL-007 as claimed in claim 1 wherein the actinomycete TL-007 is used for the inhibition of soybean black spot pathogen, soybean gray mold pathogen, cabbage black rot pathogen, ginseng fusarium pathogen and ginseng rust rot pathogen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911089241.7A CN110819561B (en) | 2019-11-08 | 2019-11-08 | Actinomycete TL-007 and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911089241.7A CN110819561B (en) | 2019-11-08 | 2019-11-08 | Actinomycete TL-007 and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110819561A CN110819561A (en) | 2020-02-21 |
| CN110819561B true CN110819561B (en) | 2021-06-11 |
Family
ID=69553725
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911089241.7A Active CN110819561B (en) | 2019-11-08 | 2019-11-08 | Actinomycete TL-007 and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110819561B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010028667A1 (en) * | 2008-09-11 | 2010-03-18 | W.C. Heraeus Gmbh | Genetically modified strains for biotransformations in anthracycline production |
| CN108949622A (en) * | 2018-07-11 | 2018-12-07 | 中国科学院东北地理与农业生态研究所 | One plant of streptomycete for producing chitinase and its application |
| CN108977382A (en) * | 2018-08-07 | 2018-12-11 | 广西壮族自治区农业科学院 | Streptomyces for producing volatile antibacterial substances and application thereof |
-
2019
- 2019-11-08 CN CN201911089241.7A patent/CN110819561B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010028667A1 (en) * | 2008-09-11 | 2010-03-18 | W.C. Heraeus Gmbh | Genetically modified strains for biotransformations in anthracycline production |
| CN108949622A (en) * | 2018-07-11 | 2018-12-07 | 中国科学院东北地理与农业生态研究所 | One plant of streptomycete for producing chitinase and its application |
| CN108977382A (en) * | 2018-08-07 | 2018-12-11 | 广西壮族自治区农业科学院 | Streptomyces for producing volatile antibacterial substances and application thereof |
Non-Patent Citations (4)
| Title |
|---|
| Antifungal activity of 3-acetylbenzamide produced by actinomycete WA23-4-4 from the intestinal tract of Periplaneta americana;Xia Fang等;《Journal of Microbiology》;20180628;第56卷;第516-523页 * |
| Enhanced antibacterial metabolite production through the application of statistical methodologies by a Streptomyces nogalater NIIST A30 isolated from Western Ghats forest soil;Jubi Jacob等;《PLos One》;20170430;第12卷(第4期);e0175919，参见全文 * |
| 一株高产抗菌活性物质链霉菌的初步分类鉴定;李德舜等;《山东大学学报》;20060430;第41卷(第2期);第144-148页 * |
| 尖孢镰刀菌人参专化型鉴定及GSK3基因和效应因子的克隆研究;王莹;《中国优秀硕士学位论文全文数据库 农业科技辑》;20170228;D046-305，参见第1-2页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110819561A (en) | 2020-02-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108034618B (en) | Siam bacillus strain and application thereof | |
| CN112458012A (en) | Bacillus belgii microbial agent and application thereof | |
| CN107136122B (en) | Biocontrol microbial inoculum for preventing and treating potato late blight | |
| CN106148232A (en) | The bacterial isolates of one strain antagonism plant pathogenetic bacteria and application thereof | |
| CN110684686A (en) | Pseudomonas fluorescens 22G5 and application thereof in preventing and treating crop verticillium wilt | |
| CN115960766A (en) | Microorganism for preventing and treating bacterial wilt and application thereof | |
| CN113549578A (en) | Siamese bacillus BsNlG13 for inhibiting rice blast germs and promoting seed germination and application thereof | |
| CN114262673A (en) | Bacillus belgii and application thereof in preventing and treating crop diseases | |
| CN111073827B (en) | Bacillus beilesensis JTB8-2 and application thereof in biological control of orobanche meloidogyne | |
| CN108220210A (en) | One plant of Antagonistic Fungi Z-18 for preventing cotton verticillium wilt and application | |
| CN113444651B (en) | Saffron endophytic fungus and application thereof in preventing and treating bulb rot | |
| CN108977385B (en) | Streptomyces mismodifier for producing chitinase and application thereof | |
| CN115197853B (en) | Endophyte Epicoccum thailandicum LF-28 strain and application thereof | |
| CN114214236B (en) | Streptomyces scorpion LSS-F01 and application thereof in crop fungal disease control | |
| CN110819561B (en) | Actinomycete TL-007 and application thereof | |
| CN112358984B (en) | Biocontrol strain YW-1 and preparation and application of biocontrol microbial inoculum thereof | |
| CN113303341B (en) | Application of streptomyces malaysiae F913 | |
| CN113373091A (en) | Biocontrol strain bacillus thuringiensis FJ2B-25 for preventing and treating rice sheath blight | |
| CN110438026B (en) | Bacillus amyloliquefaciens GLD-191 and microbial inoculum, preparation method and application thereof | |
| CN108949622B (en) | Streptomyces for producing chitinase and application thereof | |
| CN108977383B (en) | Paenibacillus for decomposing chitin and application thereof | |
| CN108277166B (en) | Trichoderma asperellum and application thereof in lead-contaminated soil remediation | |
| CN116555091B (en) | Salt-tolerant myxobacteria and application thereof | |
| CN116769667B (en) | Bacillus tequilensis K3-11 and application thereof | |
| CN113832074B (en) | Actinomycetes, and biocontrol microbial inoculum and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |