CN110814358B - Preparation method and application of Ag-Cu nano alloy with blood sugar detection characteristic - Google Patents
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Abstract
The invention discloses a preparation method and application of an Ag-Cu nano alloy with blood sugar detection characteristics, and a hydrothermal method is adopted to prepare graphene-loaded Ag 6 Cu 4 、Ag 5 Cu 5 Nanoparticles of AgNO 3 、CuSO 4 、NaBH 4 、Na 3 C 6 H 5 O 7 ·2H 2 The O and the graphene oxide are prepared by hydrothermal reaction, and the structure of the graphene oxide is spherical. A three-electrode testing method is adopted, a Saturated Calomel Electrode (SCE) is used as a reference electrode, a platinum sheet electrode is used as a counter electrode, and a Glassy Carbon Electrode (GCE) modified by Ag-Cu nano alloy particles loaded by graphene oxide is used as a working electrode. Under the alkalescent environment, the voltammetry curves of the working electrode under different scanning speeds and different blood sugar concentrations are determined by means of an electrochemical workstation. The invention adopts a hydrothermal method, and has simple process flow and low cost. The Ag-Cu nano alloy material shows excellent electrochemical characteristics and chemical stability, and can be used for measuring blood sugar concentration.
Description
Technical Field
The invention belongs to the technical field of metal nano materials, and particularly relates to a preparation method and application of an Ag-Cu nano alloy with a blood sugar detection characteristic.
Background
Glucose (also called blood sugar) is a monosaccharide which is widely distributed in nature and is the most important, and is widely applied to the fields of food industry and medical treatment. Glucose plays an important role in the field of biology, being an energy source for living cells and an intermediate product of metabolism. Glucose is easily absorbed into the blood and is of great importance to the normal functioning of the brain. The glucose concentration in blood glucose is one of the criteria for measuring the health of a human body, and if the concentration is too high, obesity and diabetes may be caused, and if the concentration is too low, hypoglycemia or insulin shock may be marked. The method has great significance for detecting the glucose, particularly for monitoring the glucose of organisms in real time, and a great deal of research is carried out on the detection of the glucose at present, wherein the method mainly comprises a chromatographic method, a spectroscopic method and an electrochemical method. Compared with the traditional analysis method, the electrochemical analysis pretreatment process is simple, the measurement operation is convenient, the electrochemical detection time is short, and even real-time monitoring can be realized. Compared with unit nanoparticles, the multi-element nano alloy has more excellent electrochemical performance, and the Ag-Cu nano alloy has the characteristics of excellent catalysis, light, electricity, surface plasma resonance, surface enhanced Raman scattering and the like. Nanda in 2016 discovered that the rate of glycerol conversion to propylene glycol was significantly increased with a copper-based catalyst containing 5% silver, and that the order of copper and silver impregnation affected the physicochemical properties of the catalyst. Jin synthesizes an Ag-Cu electrocatalyst with a dendritic morphology by taking foamed nickel as a substrate in 2015 and adopting an electrodeposition method. The catalytic performance of the catalyst in the oxidation-reduction reaction (ORR) in alkaline solution and the resistance to carbonate ions were evaluated by cyclic voltammetry linear scanning and rotating disk electrode polarization. The preparation of nano particles is usually prepared by an electrodeposition method, and the method easily causes that the precipitation rate of inorganic salt is difficult to control and the synthesized substance is difficult to be uniform.
Disclosure of Invention
In view of the above problems, the present invention is to provide a method for preparing an Ag — Cu nano alloy with blood glucose detection characteristics, and the Ag — Cu nano alloy is used for modifying a glassy carbon electrode to analyze and detect blood glucose.
In order to achieve the purpose, the invention is realized by the following technical scheme:
a preparation method of Ag-Cu nano alloy with blood sugar detection characteristics comprises the following steps:
1) mixing AgNO 3 、CuSO 4 Mixing with a sodium citrate solution to obtain a solution A;
2) adding NaBH into the solution A in an argon atmosphere 4 Magnetically stirring the solution to obtain a solution B;
3) standing the solution B in a dark place, adding an ultrasonic dispersion solution of graphene oxide, uniformly stirring, and moving to an autoclave for 4-5 hours;
4) after cooling to room temperature, the black nano powder is obtained by centrifugal separation.
Further, the molar ratio of the Ag ions to the Cu ions in the step (1) is 1: 1-3: 2.
Further, NaBH described in step (2) 4 The solution concentration was 1 mmol/L.
Further, the autoclave in the step (3) is set to have a temperature of 150 ℃ to 160 ℃.
In another aspect of the invention, the Ag-Cu nano alloy prepared by the method is applied to detecting the blood glucose concentration.
Preferably, the method for detecting the blood glucose concentration by using the Ag-Cu nano alloy comprises the following steps:
1) adding graphene-loaded Ag-Cu nano powder and a Nafion solution into absolute ethyl alcohol serving as a solvent respectively, and performing ultrasonic dispersion to obtain a modification solution;
2) polishing a glassy carbon electrode into a mirror surface, carrying out ultrasonic cleaning, and drying under infrared light;
3) placing the sample in potassium ferricyanide characterization solution for CV scanning to ensure that the potential difference between an oxidation peak and a reduction peak is within 80mV, and washing and drying the sample;
4) uniformly coating the modifying liquid on the glassy carbon electrode, and drying under infrared light;
5) the three-electrode system was placed in serum for electrochemical detection.
Further, the ultrasonic cleaning is specifically to respectively use acetone and HNO 3 And cleaning with NaOH solution and ultrapure water.
Further, the electrochemical detection specifically comprises: recording the cyclic voltammetry curve of the peak current in the range of 0.5-5.0 mmol/L of blood glucose under the sweeping speed condition of 200mV/s within the potential range of-0.7V.
The beneficial effects of the invention are as follows:
1) the blood glucose concentration is detected by an electrochemical method based on Ag-Cu nano alloy by using a hydrothermal method, the operation is simple, and the repeatability is strong;
2) in the electrochemical detection process, a glassy carbon electrode is selected as a modified electrode of a three-electrode system, and the modified interface of the glassy carbon electrode has the advantages of large modification, wide measurement range, high response sensitivity and low cost;
3) based on excellent conductivity of graphene, uniform graphene oxide dispersion liquid is used for providing a carrier, and conductivity of the modified electrode is effectively enhanced.
Drawings
FIG. 1 shows Ag loaded on graphene 5 Cu 5 TEM electron micrograph of;
FIG. 2 shows Ag loaded on graphene 6 Cu 4 TEM electron micrograph of (a);
FIG. 3 shows Ag loaded on graphene 5 Cu 5 Optimization curves of different scanning rates;
FIG. 4 shows Ag loaded on graphene 5 Cu 5 CV graphs of different concentration blood glucose solutions;
FIG. 5 shows Ag loaded on graphene 6 Cu 4 CV graphs of different concentrations of blood glucose solutions;
FIG. 6 shows Ag loaded on graphene 6 Cu 4 And Ag 5 Cu 5 The oxidation peak-to-peak current is plotted against the blood glucose concentration.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to specific embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
(1) Preparing 0.01mmol/L AgNO 3 、CuSO 4 Taking a proper amount of solution according to the molar ratio of Ag to Cu of 5 to 5, adding 3mL of sodium citrate solution with the mass fraction of 1 percent as a stabilizer, and finally preparing the Ag 5 Cu 5 100mL of the solution;
(2) adding 3mL of NaBH with the concentration of 1mmol/L into the solution prepared in the step (1) in a closed environment filled with argon 4 Magnetically stirring the solution for 20min to obtain a uniform solution;
(3) standing the solution for 1h in the dark, slowly stirring the solution, simultaneously adding 3mL of ultrasonic dispersion solution of graphene oxide with the concentration of 0.01mmol/L, and stopping stirring after uniformly stirring;
(4) transferring the mixed solution obtained in the step (3) into an autoclave, and keeping the temperature at 160 ℃ for 4 h;
(5) cooling the autoclave to room temperature, separating the metal nanoparticles in the solution by using a high-speed centrifuge (7500 rotating speed), washing for 3 times by using deionized water and absolute ethyl alcohol respectively, and carrying out vacuum drying at room temperature;
(6) 0.5mg of each graphene oxide is loaded with Ag 5 Cu 5 Dissolving the nano powder sample in 1ml of absolute ethyl alcohol to prepare Ag 5 Cu 5 A solution;
(7) adding 10ul of 10 wt% Nafion solution into the obtained solution, and performing ultrasonic dispersion for 20 minutes to obtain a uniformly dispersed modification solution;
(8) polishing glassy carbon electrode on 2000Cw metallographic abrasive paper to remove surface residual substances, and sequentially painting 0.3 and 0.05 μm of alpha-Al on dry and wet chamois leather pads according to' inverse 8-character painting method 2 O 3 Polishing the polishing powder into a mirror surface, sequentially and respectively adding acetone and HNO 3 (1:1, v/v), NaOH (1M) and ultrapure water are subjected to ultrasonic cleaning for 5min and then placed under infrared lamp light for drying;
(9) performing CV scanning in a potassium ferricyanide characterization solution to ensure that the potential difference between an oxidation peak and a reduction peak is within 80mV, checking whether the surface of the electrode is clean, and washing and drying for three times;
(10) using a pipette to take 15ul of the modified solution, uniformly dripping the solution on a glassy carbon electrode for 3-4 times, and marking the solution as GCE/Ag 5 Cu 5 NPs, drying under infrared light;
(11) and (3) placing the three-electrode system in the treated serum, and recording a cyclic voltammetry curve of the peak current in the range of 0.5-5.0 mmol/L of blood glucose under the sweeping speed condition of 200mV/s within the potential range of-0.7V.
Example 2
(1) Preparing 0.01mmol/L AgNO 3 、CuSO 4 Taking a proper amount of solution according to the molar ratio of Ag to Cu of 6 to 4, adding 3mL of sodium citrate solution with the mass fraction of 1 percent as a stabilizer, and finally preparing the Ag 6 Cu 4 100mL of the solution;
(2) adding 3mL of NaBH with the concentration of 1mmol/L into the solution prepared in the step (1) in a closed environment filled with argon 4 Magnetically stirring the solution for 20min to obtain a uniform solution;
(3) standing the solution for 1h in the dark, slowly stirring the solution, simultaneously adding 3mL of ultrasonic dispersion solution of graphene oxide with the concentration of 0.01mmol/L, and stopping stirring after uniformly stirring;
(4) transferring the mixed solution obtained in the step (3) into an autoclave, and keeping the temperature at 160 ℃ for 4 h;
(5) cooling the autoclave to room temperature, separating the metal nanoparticles from the solution by a high-speed centrifuge (7500 rpm), washing with deionized water and absolute ethanol for 3 times, respectively, and vacuum drying at room temperature.
(6) 0.5mg of each graphene oxide is loaded with Ag 6 Cu 4 Dissolving the nano powder sample in 1ml of absolute ethyl alcohol to prepare Ag 6 Cu 4 A solution;
(7) adding 10ul of 10 wt% Nafion solution into the obtained solution, and performing ultrasonic dispersion for 20 minutes to obtain a uniformly dispersed modification solution;
(8) polishing glassy carbon electrode on 2000Cw metallographic abrasive paper to remove surface residual substances, and sequentially painting 0.3 and 0.05 μm of alpha-Al on dry and wet chamois leather pads according to' inverse 8-character painting method 2 O 3 Polishing the polishing powder into a mirror surface, sequentially and respectively adding acetone and HNO 3 (1:1, v/v), NaOH (1M) and ultrapure water are subjected to ultrasonic cleaning for 5min and then placed under infrared lamp light for drying;
(9) performing CV scanning in a potassium ferricyanide characterization solution to ensure that the potential difference between an oxidation peak and a reduction peak is within 80mV, checking whether the surface of the electrode is clean, and washing and drying for three times;
(10) using a pipette to take 15ul of the modified solution, uniformly dripping the solution on a glassy carbon electrode for 3-4 times, and marking the solution as GCE/Ag 6 Cu 4 NPs, drying under infrared light;
(11) and (3) placing the three-electrode system in the treated serum, and recording a cyclic voltammetry curve of the peak current in the range of 0.5-5.0 mmol/L of blood glucose under the sweeping speed condition of 200mV/s within the potential range of-0.7V.
Example 3
To verify that graphene oxide supports Ag 5 Cu 5 The nanometer alloy modified electrode has obvious effect on blood glucose concentration detection, and Ag is loaded on the graphene oxide according to the method 5 Cu 5 Preparation method of nano alloy for preparing graphene oxide loaded Ag 6 Cu 4 The nano alloy, the steps for special description are the same as the preparation method, except that: ag. Au accounts for different proportions and is respectively Ag 5 Cu 5 And Ag 6 Cu 4 。
The transmission electron microscope of the graphene oxide-supported Ag5Cu5 nano alloy prepared in example 1 is shown in fig. 1, and the transmission electron microscope of the graphene oxide-supported Ag6Cu4 nano alloy prepared in example 2 is shown in fig. 2, and the obtained nano alloy is in a spherical structure.
The specific method for detecting the blood glucose concentration by using the Ag-Cu spherical nano alloy comprises the following steps: and dripping the obtained Ag-Cu nano alloy modified solution on the surface of the glassy carbon electrode and drying to prepare a modified electrode, and optimizing the blood glucose concentration and the scanning rate in serum, wherein the optimization result is shown in figure 3. Cyclic voltammograms were measured at the optimized scan rate in different concentrations of serum.
As shown in fig. 4 and 5, the following results were obtained: testing the CV curve of the graphene oxide loaded Ag in the potential range of-0.7V and the scanning rate of 200mV/s 5 Cu 5 When the electrode is modified by the nano alloy, the oxidation peak current is more than 3 x 10 when the blood sugar concentration is 0.5mmol/L -4 A, when the electrode has blood sugar concentration of 5mmol/L, the oxidation peak current is closer to 8 x 10 -4 A, as shown in FIG. 6, the oxidation peak response current has a good linear relationship with the blood glucose concentration, and the linear equation obtained by fitting is: Y-3E-12X 2+ E-4X + 2E-4. Ag 6 Cu 4 The oxidation peak current of the glassy carbon electrode modified by the nano particles is only 1.6 x 10 at the blood glucose concentration of 5mmol/L -4 A shows that the graphene oxide supports Ag 5 Cu 5 The nano alloy has higher selectivity and sensitivity to the detection of the blood glucose concentration.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (5)
1. A preparation method of Ag-Cu nano alloy with blood sugar detection characteristics is characterized by comprising the following steps:
1) mixing AgNO 3 、CuSO 4 Mixing with a sodium citrate solution to obtain a solution A; wherein the molar ratio of Ag ions to Cu ions is 1: 1-3: 2;
2) adding 1 mmol/LNaBH into the solution A in an argon atmosphere 4 Magnetically stirring the solution to obtain a solution B;
3) standing the solution B in a dark place, adding an ultrasonic dispersion solution of graphene oxide, uniformly stirring, transferring to an autoclave at the temperature of 150-160 ℃, and keeping for 4-5 hours;
4) after cooling to room temperature, the black nano powder is obtained by centrifugal separation.
2. The use of the Ag-Cu nano alloy prepared by the preparation method of claim 1 in detecting blood glucose concentration.
3. Use according to claim 2, characterized in that it comprises the following steps:
1) adding the graphene oxide-loaded Ag-Cu nano powder and a Nafion solution into absolute ethyl alcohol serving as a solvent respectively, and performing ultrasonic dispersion to obtain a modification solution;
2) polishing a glassy carbon electrode into a mirror surface, carrying out ultrasonic cleaning, and drying under infrared light;
3) placing the sample in potassium ferricyanide characterization solution for CV scanning to ensure that the potential difference between an oxidation peak and a reduction peak is within 80mV, and washing and drying the sample;
4) uniformly coating the modifying liquid on the glassy carbon electrode, and drying under infrared light;
5) the three-electrode system was placed in serum for electrochemical detection.
4. Use according to claim 3, wherein the ultrasonic cleaning is carried out with acetone, HNO, respectively 3 And cleaning with NaOH solution and ultrapure water.
5. Use according to claim 3, wherein the electrochemical detection is in particular: recording the cyclic voltammetry curve of the peak current in the range of 0.5-5.0 mmol/L of blood glucose under the sweeping speed condition of 200mV/s within the potential range of-0.7V.
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20100057003A (en) * | 2010-05-10 | 2010-05-28 | 주식회사 패턴크리트 | Pt-ag-cyd antimicrobial and producing method of the same |
| TW201300547A (en) * | 2011-06-30 | 2013-01-01 | Hon Hai Prec Ind Co Ltd | Graphene added magnesium alloy and magnesium alloy structural member using same |
| CN103466611A (en) * | 2013-09-29 | 2013-12-25 | 黑龙江大学 | Method for preparing graphene load nano silver-nickel alloy composite powder materials |
| CN104475753A (en) * | 2014-12-29 | 2015-04-01 | 黑龙江大学 | Method for preparing nano Cu3.8 Ni alloy loaded on graphene by liquid phase reduction method |
| CN106028768A (en) * | 2016-05-17 | 2016-10-12 | 国网重庆市电力公司电力科学研究院 | Iron-plated graphene and preparation method |
| CN108052794A (en) * | 2018-01-16 | 2018-05-18 | 桂林电子科技大学 | The Forecasting Methodology of the eutectic temperature of various sizes of nanometer Ag-Cu alloy systems |
| WO2018201715A1 (en) * | 2017-05-03 | 2018-11-08 | 广州特种承压设备检测研究院 | Graphene and ferroferric oxide@gold composite material and preparation method and application thereof |
| CN108786845A (en) * | 2018-06-27 | 2018-11-13 | 济南大学 | A kind of preparation method of dendroid Pt-Ni-Cu alloy nanoparticles |
| CN109546168A (en) * | 2018-11-22 | 2019-03-29 | 龙岩学院 | A kind of carbon material supported silver-colored platinum Nanoalloy composite material and preparation method |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012018777A1 (en) * | 2010-08-03 | 2012-02-09 | University Of Connecticut | Non-enzymatic glucose sensors based on metal oxide nanomaterials |
| US9725614B2 (en) * | 2013-04-19 | 2017-08-08 | Xerox Corporation | Conductive ink compositions and methods for preparation of stabilized metal-containing nanoparticles |
| CN103954661B (en) * | 2014-05-05 | 2016-01-06 | 兰州大学 | A kind of silver of the copper for glucose sensor binary composite nano material and preparation method thereof |
| CN105403604B (en) * | 2015-12-17 | 2018-04-10 | 河南省科学院能源研究所有限公司 | Enzyme-free glucose electrochemical sensor based on metal nanoparticle/nano-cellulose compound |
| CN107389767B (en) * | 2017-06-09 | 2019-09-27 | 上海交通大学 | Based on nano silver-preparation method of the copper oxide particle/graphene without enzyme electrochemical glucose sensor |
| KR102130066B1 (en) * | 2018-03-08 | 2020-07-06 | 재단법인대구경북과학기술원 | An electrode having hierarchical structure, a semsor for ditecting glucose including the electrode, and a method for preparing the electrode |
| CN110057880A (en) * | 2019-05-09 | 2019-07-26 | 武汉大学 | A kind of preparation method of the flexible enzyme-free glucose electrochemical sensor based on copper-silver bimetallic |
-
2019
- 2019-11-15 CN CN201911121468.5A patent/CN110814358B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20100057003A (en) * | 2010-05-10 | 2010-05-28 | 주식회사 패턴크리트 | Pt-ag-cyd antimicrobial and producing method of the same |
| TW201300547A (en) * | 2011-06-30 | 2013-01-01 | Hon Hai Prec Ind Co Ltd | Graphene added magnesium alloy and magnesium alloy structural member using same |
| CN103466611A (en) * | 2013-09-29 | 2013-12-25 | 黑龙江大学 | Method for preparing graphene load nano silver-nickel alloy composite powder materials |
| CN104475753A (en) * | 2014-12-29 | 2015-04-01 | 黑龙江大学 | Method for preparing nano Cu3.8 Ni alloy loaded on graphene by liquid phase reduction method |
| CN106028768A (en) * | 2016-05-17 | 2016-10-12 | 国网重庆市电力公司电力科学研究院 | Iron-plated graphene and preparation method |
| WO2018201715A1 (en) * | 2017-05-03 | 2018-11-08 | 广州特种承压设备检测研究院 | Graphene and ferroferric oxide@gold composite material and preparation method and application thereof |
| CN108052794A (en) * | 2018-01-16 | 2018-05-18 | 桂林电子科技大学 | The Forecasting Methodology of the eutectic temperature of various sizes of nanometer Ag-Cu alloy systems |
| CN108786845A (en) * | 2018-06-27 | 2018-11-13 | 济南大学 | A kind of preparation method of dendroid Pt-Ni-Cu alloy nanoparticles |
| CN109546168A (en) * | 2018-11-22 | 2019-03-29 | 龙岩学院 | A kind of carbon material supported silver-colored platinum Nanoalloy composite material and preparation method |
Non-Patent Citations (2)
| Title |
|---|
| Low temperature synthesis and thermal properties of Ag−Cu alloy nanoparticles;W.BHAGATHSINGH等;《Transactions of Nonferrous Metals Society of China》;20130208;第23卷(第1期);第128-133页 * |
| 基于核酸探针和纳米酶的生物传感设计及其在癌症标志物检测中的应用;徐齐;《中国优秀博硕士学位论文全文数据库(硕士) 医药卫生科技辑》;20190615(第06期);第E072-12页 * |
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