CN110794018B

CN110794018B - Biological sensing element, device and biosensor

Info

Publication number: CN110794018B
Application number: CN201911088356.4A
Authority: CN
Inventors: 周敏; 刘耀; 李宗祥; 陶文昌; 洪贵春; 吴振钿; 王进; 石常洪; 邱鑫茂; 庄子华; 程浩; 吕耀朝; 刘祖文; 林剑涛; 朱敬光; 林琳琳; 黄雅雯
Original assignee: BOE Technology Group Co Ltd; Fuzhou BOE Optoelectronics Technology Co Ltd
Current assignee: BOE Technology Group Co Ltd; Fuzhou BOE Optoelectronics Technology Co Ltd
Priority date: 2019-11-08
Filing date: 2019-11-08
Publication date: 2023-03-31
Anticipated expiration: 2039-11-08
Also published as: CN110794018A

Abstract

The invention discloses a biosensor element, a device and a biosensor, wherein the element comprises: the organic light emitting diode OLED layer is characterized in that the cathode of the OLED layer is a graphene layer with active groups and is configured to be combined with biomolecules to be detected; and the photoelectric transmission PIN layer is arranged on one side of the OLED layer, which is far away from the cathode, and is configured to receive light emitted by the OLED and output current with corresponding magnitude according to the intensity of the light. According to the invention, the cathode of the OLED is set to be the graphene layer with active groups, the to-be-detected biomolecules are adsorbed by the cathode of the OLED, so that the electron transmission rate is changed, the luminous brightness of the OLED is changed under the action of bias voltage, the brightness change is captured by the PIN, currents with different sizes are output, various to-be-detected information of the to-be-detected biomolecules is obtained according to the current sizes, the detection of biological signals is converted into the detection of electric signals, and the detection sensitivity and accuracy are improved.

Description

Biological sensing element, device and biosensor

Technical Field

The invention relates to the field of biosensing, in particular to a biosensing element, a biosensing device and a biosensor.

Background

At present, biosensors mainly comprise a semiconductor biosensor, an electrochemical biosensor, a solid electrolyte biosensor, a polymer biosensor and the like, but the conventional biosensors mainly perform chemical reaction with a sensor channel or an electrode based on a biological substance to be detected to detect the biological substance to be detected, so that not only can a biological substance sample and the sensor to be detected be damaged, but also the biological substance sample and the sensor are greatly influenced by factors such as illumination, environment and the like, and a detection result is easily influenced, so that the detection accuracy is low.

Disclosure of Invention

The embodiment of the invention aims to provide a biosensor element, a biosensor device and a biosensor, and aims to solve the problems that in the prior art, the biosensor is mainly used for detecting through chemical reaction, is greatly influenced by the environment and has low accuracy.

In order to solve the technical problem, the embodiment of the application adopts the following technical scheme: a biosensing element, comprising: an Organic Light Emitting Diode (OLED) layer, wherein the cathode of the OLED layer is a graphene layer with active groups and is configured to be combined with the biomolecule to be detected; and the PIN (photoelectric transmission) layer is arranged on one side of the OLED layer far away from the cathode, is configured to receive the light emitted by the OLED and output current with corresponding magnitude according to the intensity of the light.

Further, a transparent insulating layer is arranged between the OLED layer and the PIN layer.

Further, still include: and the processor is configured to receive the current output by the PIN layer and determine the information to be detected of the biomolecule to be detected according to the current.

Further, still include: the processor includes at least: an integrated read circuit.

Further, the cathode of the OLED layer is a graphene layer subjected to surface treatment by plasma.

The embodiment of the invention also discloses a biosensor device, which comprises: a thin film transistor and the biosensing element according to any one of claims 1 to 5; the thin film transistor is arranged on one side, away from the cathode, of the OLED layer, a first pole of the thin film transistor is connected with the PIN layer and is configured to receive current output by the PIN layer, a second pole of the thin film transistor is connected with the processor and is configured to transmit the current to the processor under the condition that a grid electrode of the thin film transistor is opened, wherein the first pole is different from the second pole, and the first pole and the second pole are source electrodes or drain electrodes.

Further, a metal shielding layer is arranged between the OLED layer and the thin film transistor.

The embodiment of the invention also discloses a biosensor, which is characterized by comprising the following components: at least one biosensing device according to claim 6 or 7 arranged in an array; a gate line connected to a gate electrode of a thin film transistor of the biosensor device, configured to apply a voltage to the gate electrode; and the data line is connected with the second pole of the thin film transistor of the biological sensing device and is configured to output the current output by the PIN layer.

Further, still include: and the processor is connected with the data line and is configured to determine the information of the biomolecules to be detected according to the current output by the PIN layer.

Furthermore, the grid electrodes of the thin film transistors of all the biosensing devices in any row are connected with the same grid line; the second poles of the thin film transistors of all the biosensing devices in any column are connected with the same data line.

The embodiment of the invention has the beneficial effects that: the cathode of the OLED is set to be the graphene layer with active groups, the biomolecules to be detected are adsorbed by the cathode of the OLED, so that the electron transmission rate is changed, the luminous brightness of the OLED is changed under the action of bias voltage, the current with different brightness changes is output by capturing the brightness changes by the PIN, various pieces of information to be detected of the biomolecules to be detected are obtained according to the current, the detection of converting the detection of biological signals into electric signals is achieved, and the detection sensitivity and accuracy are improved.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below, it is obvious that the drawings in the following description are only some embodiments described in the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

FIG. 1 is a schematic structural diagram of a biosensor device according to a first embodiment of the present invention;

FIG. 2 is a schematic structural diagram of an OLED layer according to a first embodiment of the present invention;

FIG. 3 is a schematic diagram showing the binding of a protein molecule to a graphene surface active group according to a first embodiment of the present invention;

FIG. 4 is a schematic structural diagram of a PIN layer in the first embodiment of the present invention;

FIG. 5 is a schematic view showing the construction of a biosensor device in a second embodiment of the present invention;

FIG. 6 is a perspective view of a biosensor device according to a second embodiment of the present invention;

FIG. 7 is a schematic view showing the arrangement of biosensors in the third embodiment of the present invention.

Detailed Description

Various aspects and features of the present application are described herein with reference to the drawings.

It will be understood that various modifications may be made to the embodiments of the present application. Accordingly, the foregoing description should not be construed as limiting, but merely as exemplifications of embodiments. Those skilled in the art will envision other modifications within the scope and spirit of the application.

The accompanying drawings, which are incorporated in and constitute a part of the specification, illustrate embodiments of the application and, together with a general description of the application given above and the detailed description of the embodiments given below, serve to explain the principles of the application.

These and other characteristics of the present application will become apparent from the following description of preferred forms of embodiment, given as non-limiting examples, with reference to the attached drawings.

It should also be understood that, although the present application has been described with reference to some specific examples, a person of skill in the art shall certainly be able to achieve many other equivalent forms of application, having the characteristics as set forth in the claims and hence all coming within the field of protection defined thereby.

The above and other aspects, features and advantages of the present application will become more apparent in view of the following detailed description when taken in conjunction with the accompanying drawings.

Specific embodiments of the present application are described hereinafter with reference to the accompanying drawings; however, it is to be understood that the disclosed embodiments are merely exemplary of the application, which can be embodied in various forms. Well-known and/or repeated functions and constructions are not described in detail to avoid obscuring the application of unnecessary or unnecessary detail. Therefore, specific structural and functional details disclosed herein are not to be interpreted as limiting, but merely as a basis for the claims and as a representative basis for teaching one skilled in the art to variously employ the present application in virtually any appropriately detailed structure.

The specification may use the phrases "in one embodiment," "in another embodiment," "in yet another embodiment," or "in other embodiments," which may each refer to one or more of the same or different embodiments in accordance with the application.

A first embodiment of the present invention provides a biosensor, which is mainly used for detecting biomolecules, and a schematic structural diagram of the biosensor is shown in fig. 1, and mainly includes: an Organic Light-Emitting Diode (OLED) layer 100 and a photoelectric transmission PIN layer 200, wherein a cathode of the OLED layer 100 is a graphene layer having active groups (an upper surface of the OLED layer 100 in fig. 1 is a cathode surface), and is configured to contact and bind with a biomolecule to be detected; the PIN layer 200 is disposed on a side of the OLED layer 100 away from the cathode (in fig. 1, the side below the OLED layer 100 is away from the cathode), and the PIN layer 200 is configured to receive light emitted from the OLED layer 100 and output a current of a corresponding magnitude according to the intensity of the light.

Specifically, fig. 2 is a schematic structural diagram of an OLED layer, which includes a cathode 101, an emitting layer 102, a hole transport layer 103, a hole injection layer 104, and an anode 105, which are sequentially arranged from top to bottom in fig. 2. When the OLED layer is manufactured, a conductive glass (ITO) may be first deposited on a glass substrate (not shown in fig. 2) to serve as an anode 105, then an organic film layer hole injection layer 104, a hole transport layer 103, and a light emitting layer 102 are sequentially vacuum-evaporated, and finally a film-formed graphene is used as a cathode 101, and plasma nitrogen N is passed through the graphene to form the OLED layer ₂ Hydrogen gas H ₂ Oxygen O ₂ And performing surface treatment on the gas molecules to ensure that the surfaces of the gas molecules are rich in active groups for modification and are used for binding biological molecules such as proteins. In practical use, the biomolecule to be detected existing in a liquid form is dripped on the upper surface of the cathode 101, taking the biomolecule to be detected as protein as an example, the graphene can promote electron transfer and has good biocompatibility, when the protein molecule is contacted with the graphene layer with active groups, as shown in fig. 3, the protein molecule is combined with the active groups on the graphene surface for modification, so that the electron transfer and migration rates of the OLED layer are changed, and under the action of bias voltageThe luminance of the OLED layer changes, and the luminance of the OLED layer changes along with the combination of more protein molecules and active groups on the surface of the graphene, which can be modified.

Fig. 4 shows a schematic structural diagram of a photoelectric transmission layer 200, which mainly includes a package layer 201, a P-type semiconductor 202, an I-type semiconductor 203, and an N-type semiconductor 204, which are sequentially arranged from top to bottom in fig. 4, wherein the package layer 201 may preferably use an ITO material, and is mainly used for biasing PIN and preventing oxidation, the P-type semiconductor 202 is mainly a silicon crystal added with a trivalent element, the N-type semiconductor 204 is mainly a silicon crystal added with a pentavalent element, and the I-type semiconductor 203 is an intrinsic semiconductor and is a pure semiconductor completely free of impurities and lattice defects, in this embodiment, the silicon crystal is also used as the I-type semiconductor, and the I-type semiconductor is thicker and is used for widening a depletion layer, reducing junction capacitance, widening absorption in a long-wave region, and greatly accelerating a response speed of the PIN layer 200. In addition, a transparent insulating layer (not shown in fig. 1) should be disposed between the OLED layer 100 and the PIN layer 200 to realize insulating encapsulation therebetween, the insulating layer is generally made of silicon nitride (SiNx), the number of specific layers of the insulating layer is also set according to actual conditions, and two or more insulating layers can be used in combination to achieve a better insulating effect.

In practical use, the cathode 101 of the OLED layer 100 adsorbs the biomolecules to be detected to change the electron transmission rate, the brightness of the OLED layer 100 is changed under the action of bias voltage, the brightness change is captured by the PIN layer 200 to output currents with different magnitudes, and the information to be detected of the biomolecules to be detected is determined by processing the currents output by the PIN layer 200. Specifically, the information to be detected of the biomolecule to be detected may include at least any one of the following information: whether the biomolecule to be detected exists in the liquid drop to be detected, the type of the biomolecule to be detected, the number of the biomolecule to be detected, the concentration of the biomolecule to be detected in the liquid drop to be detected, and the like. In order to realize the detection of the biomolecule to be detected, the biosensor in this embodiment further includes a processor (not shown in fig. 1), connected to the PIN layer 200, for receiving the current output by the PIN layer 200 and determining the above-mentioned various items of information to be detected of the biomolecule to be detected according to the current. In particular, the processor may be a processing device including more than one general purpose processing device, such as a microprocessor, central Processing Unit (CPU), graphics Processing Unit (GPU), or the like. More specifically, the processor may be a Complex Instruction Set Computing (CISC) microprocessor, reduced Instruction Set Computing (RISC) microprocessor, very Long Instruction Word (VLIW) microprocessor, processor running other instruction sets, or processors running a combination of instruction sets. The processor may also be one or more special-purpose processing devices such as an Application Specific Integrated Circuit (ASIC), a Field Programmable Gate Array (FPGA), a Digital Signal Processor (DSP), system on chip (SoC), or the like. In this embodiment, the processor includes at least an Integrated Read Circuit (ROIC) for reading The change in charge in The current output by The PIN layer.

Further, after the detection is finished, the cathode 101 of the OLED layer 100 is biased to release the adsorbed biomolecules to be detected, so that the biosensor can be used for detection again without irreversible chemical reaction, thereby increasing the service life of the biosensor.

Set up OLED's negative pole into the graphite alkene layer that has the active group in this embodiment, adsorb through OLED's negative pole and wait to detect biomolecule and make electron transmission rate change, under the bias voltage effect, make OLED's luminance change, thereby caught the not electric current of equidimension of luminance change output by PIN, obtain again according to the size of electric current and wait to detect each item information that awaits measuring of detecting biomolecule, realize converting the detection of telecommunication into from biosignal, the sensitivity and the rate of accuracy of detection have been promoted, meanwhile, the biosensing component of this embodiment is made in the integration of the current production line of accessible, and the manufacturing cost is reduced.

A second embodiment of the present invention provides a biosensor device, which is added with a Thin Film Transistor (TFT) on a side of the OLED layer away from the cathode, wherein a first pole of the TFT is connected to the PIN layer and configured to receive a current output by the PIN layer, and a second pole of the TFT is connected to the processor and configured to transmit the current to the processor when the gate of the TFT is turned on, wherein the first pole and the second pole are different and are source electrodes or drain electrodes, and the detection control of the biosensor device is realized by controlling the turn-on and turn-off of the gate of the TFT. In practical use, a metal shielding layer is arranged between the OLED layer and the thin film transistor to shield the channel of the thin film transistor from light and prevent the channel from being influenced by illumination.

The schematic structural diagram of the biosensor device in this embodiment is shown in fig. 5, and mainly includes: the thin film transistor comprises an OLED layer 100, a PIN layer 200, an insulating layer 400, a metal shielding layer 500, a resin layer 600, a transfer layer 700, a first pole 301 of a TFT, a channel layer 302 of the TFT, a second pole 303 of the TFT, a gate 304 of the TFT, and a substrate 800, wherein the first pole 301 of the TFT, the channel layer 302 of the TFT, the second pole 303 of the TFT, the gate 304 of the TFT, and the insulating layer 400 between the channel layer 302 and the gate 304 form the whole structure of the thin film transistor 300.

Specifically, the specific functions of the OLED layer 100 and the PIN layer 200 have been described in the first embodiment, and are not repeated herein; the insulating layer 400, which may also be referred to as a passivation layer, is generally made of a silicon nitride material and is used to fill the space between the layers for insulation, and it should be noted that the insulating layer 400 disposed between the OLED layer 100 and the PIN layer 200 should be made of a transparent material to ensure that the PIN layer 200 can acquire light emitted from the OLED layer 100; the metal shielding layer 500 is disposed between the OLED layer 100 and the thin film transistor 300, and the size of the metal shielding layer is based on the size of the TFT, so that the channel layer 302 can be shielded; the resin layer 600 can be used as a composite insulating layer as well as a planarization layer; the transport layer 700 may be practically any metal layer connecting the first pole 301 of the TFT with the N-type semiconductor (not shown in fig. 5) of the PIN layer 200, primarily acting to transport the current generated by the PIN layer 200 to the TFT300 device, and secondarily acting to bias the PIN layer 200 and to encapsulate it against oxidation; the first electrode 301 of the TFT, which may be a source electrode or a drain electrode of the TFT, is connected to the N-type semiconductor of the PIN layer 200 through the transmission layer 700, and receives a current output from the N-type semiconductor of the PIN layer 200; the channel layer 302 of the TFT is generally made of a semiconductor material such as amorphous silicon (a-Si), indium Gallium Zinc Oxide (IGZO), and is used to transmit current on the first pole 302 to the second pole 303 when a voltage is applied to the gate electrode 304; the second pole 303 may also be a source or a drain of the TFT, and when the first pole 302 is a source, the second pole 304 is a drain, or vice versa, and an end thereof not connected to the channel layer 303 may be connected to a processor or to a ROIC circuit in the processor for outputting a current generated by the PIN layer; the gate 304 is used to control the on and off of the channel layer 303, and is typically controlled by a gate line; the substrate 800 is a device carrier, such as a hard carrier like glass, or a flexible carrier like Polyethylene terephthalate (PET) material.

Fig. 6 shows a perspective view of a biosensor device, which specifically shows the size and position relationship between the TFT300 and the PIN layer 200, that is, a TFT300 is disposed in each biosensor device for controlling the biosensor device, that is, the current of the biosensor device can be outputted and turned off according to actual requirements during actual detection, thereby improving the fine control of the biosensor device. In addition, the TFT, the OLED and the PIN can be produced by using the existing production line, and the manufacturing cost is low.

The third embodiment of the present invention provides a biosensor, the arrangement schematic diagram of which is shown in fig. 7, and mainly includes: at least one biosensing device 10 as described in the second embodiment of the present invention arranged in an array; a gate line 20 connected to a gate electrode of a thin film transistor (shown as a shaded square in fig. 7) of the biosensor device, for applying a voltage to the gate electrode to control the channel to be turned on or off; and a data line 30 connected to a second pole of the thin film transistor of the bio sensor device, for outputting the current outputted from the PIN layer. The specific structure and action of the biosensing device 10 have been described in the second embodiment of the present invention, and thus detailed description of the specific structure and action of the biosensing device 10 will not be repeated in this embodiment.

In addition, the biosensor may further include a processor (not shown in fig. 7) connected to all the data lines for determining information of the bio-molecules to be detected according to the current output from the PIN layer. More specifically, the processor may be a Complex Instruction Set Computing (CISC) microprocessor, reduced Instruction Set Computing (RISC) microprocessor, very Long Instruction Word (VLIW) microprocessor, processor running other instruction sets, or processors running a combination of instruction sets. The processor may also be one or more special-purpose processing devices such as an Application Specific Integrated Circuit (ASIC), a Field Programmable Gate Array (FPGA), a Digital Signal Processor (DSP), a system on a chip (SoC), or the like. In this embodiment, the processor includes at least an Integrated Read Circuit (ROIC) for reading The change in charge in The current output by The PIN layer.

As shown in fig. 7, a schematic diagram of four biosensing devices 10 arranged in an array is shown, the number of the biosensing devices 10 is usually thousands in practical use, and the array arrangement is selected according to practical requirements and equipment limitations, and fig. 7 provides only a preferred arrangement schematic diagram, and does not limit the embodiment of the present invention. In this embodiment, the gate lines 20 may be configured as lines extending left and right, that is, the gates of the thin film transistors of all the biosensing devices in any row are connected to the same gate line 20, that is, when a voltage is applied to one gate line 20, the gates of all the biosensing devices 10 connected to the gate line 20 can simultaneously control the channel to be opened; the data line 30 may be configured as a vertically extending line, that is, the second poles of the thin film transistors of all the biosensor devices in any one column are all connected to the same data line 30, so as to ensure that when all the biosensor devices 10 in the same row are simultaneously turned on, each biosensor device can output corresponding current based on different data lines 30, and thus, the determination of the information to be detected of the biomolecule to be detected, which is detected by each biosensor device 10, can be accurately achieved. It should be understood that, in actual use, the gate line 20 may be configured to extend up and down, and at this time, it is only required to ensure that the gates of the thin film transistors of all the biosensing devices in any one column are all connected to the same gate line 20, and correspondingly, the data line 30 is configured to extend left and right at this time, and at this time, the second poles of the thin film transistors of all the biosensing devices in any one row are all connected to the same data line 30, and the same effect can also be achieved.

This embodiment is through arranging biosensor device array, combines the grid line to biosensor device's on-off control, can realize that biosensor treats and detects biomolecule and carry out the detection of timing ration, and then promotes biosensor's detection precision and degree of accuracy, reaches better detection effect. Meanwhile, the TFT, the OLED and the PIN can be produced by using the existing production line, so that the manufacturing cost can be reduced.

The foregoing description is only exemplary of the preferred embodiments of the invention and is illustrative of the principles of the technology employed. It will be appreciated by those skilled in the art that the scope of the disclosure herein is not limited to the particular combination of features described above, but also encompasses other combinations of features described above or equivalents thereof without departing from the spirit of the disclosure. For example, the above features and the technical features (but not limited to) having similar functions disclosed in the present invention are mutually replaced to form the technical solution.

Although the subject matter has been described in language specific to structural features and/or methodological acts, it is to be understood that the subject matter defined in the appended claims is not necessarily limited to the specific features or acts described above. Rather, the specific features and acts described above are disclosed as example forms of implementing the claims.

While the embodiments of the present invention have been described in detail, the present invention is not limited to these specific embodiments, and those skilled in the art can make various modifications and modifications of the embodiments based on the concept of the present invention, which fall within the scope of the present invention as claimed.

Claims

1. A biosensor device, comprising:

the Organic Light Emitting Diode (OLED) layer at least comprises a cathode, a light emitting layer, a hole transport layer, a hole injection layer and an anode, wherein the cathode is a graphene layer with active groups, the surface of the graphene layer is processed to be rich in the active groups for modification, and the graphene layer is configured to be combined with biomolecules to be detected;

and the photoelectric transmission PIN layer is arranged on one side of the OLED layer, which is far away from the cathode, and is configured to receive the light emitted by the OLED and output current with corresponding magnitude according to the light intensity.

2. The biosensor of claim 1, wherein a transparent insulating layer is disposed between the OLED layer and the PIN layer.

3. The biosensor of claim 1, further comprising: and the processor is configured to receive the current output by the PIN layer and determine the information to be detected of the biomolecule to be detected according to the current.

4. The biosensing element of claim 3, further comprising: the processor includes at least: an integrated read circuit.

5. The biosensor of any of claims 1 to 4, wherein the cathode of the OLED layer is a graphene layer that has been surface treated with plasma.

6. A biosensor device, comprising:

a thin film transistor and the biosensing element according to any one of claims 1 to 5; wherein, the first and the second end of the pipe are connected with each other,

the thin film transistor is arranged on one side, far away from the cathode, of the OLED layer, a first pole of the thin film transistor is connected with the PIN layer and is configured to receive current output by the PIN layer, a second pole of the thin film transistor is connected with the processor and is configured to transmit the current to the processor under the condition that a grid electrode of the thin film transistor is opened, wherein the first pole is different from the second pole, and the first pole and the second pole are source electrodes or drain electrodes.

7. The biosensing device of claim 6, wherein a metal shielding layer is disposed between said OLED layer and said thin film transistor.

8. A biosensor, comprising:

at least one biosensing device according to claim 6 or 7 arranged in an array;

a gate line connected to a gate electrode of a thin film transistor of the biosensor device, configured to apply a voltage to the gate electrode;

and the data line is connected with the second pole of the thin film transistor of the biological sensing device and is configured to output the current output by the PIN layer.

9. The biosensor of claim 8, further comprising: and the processor is connected with the data line and is configured to determine the information of the biomolecules to be detected according to the current output by the PIN layer.

10. The biosensor as claimed in claim 9, wherein the gates of the thin film transistors of all the biosensor devices in any one row are connected to the same gate line; the second poles of the thin film transistors of all the biosensing devices in any column are connected with the same data line.