CN110771620A - Application of coronatine in inducing crop seedlings to defend prodenia litura - Google Patents
Application of coronatine in inducing crop seedlings to defend prodenia litura Download PDFInfo
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- CN110771620A CN110771620A CN201911065812.3A CN201911065812A CN110771620A CN 110771620 A CN110771620 A CN 110771620A CN 201911065812 A CN201911065812 A CN 201911065812A CN 110771620 A CN110771620 A CN 110771620A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N53/00—Biocides, pest repellants or attractants, or plant growth regulators containing cyclopropane carboxylic acids or derivatives thereof
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Abstract
The invention relates to application of coronatine in inducing crop seedlings to defend prodenia litura, and belongs to the technical field of green prevention and control of crop diseases and insect pests. The invention aims to provide a new application of coronatine, namely an application of coronatine in inducing crop seedlings to defend prodenia litura. The coronatine can induce crop seedling defense, so that the growth and development of spodoptera litura larvae inhabiting on crops are inhibited or delayed, the dosage is small, the effect is good, the environmental pollution is small, and a new choice is provided for green prevention and control of spodoptera litura.
Description
Technical Field
The invention relates to application of coronatine in inducing crop seedlings to defend prodenia litura, and belongs to the technical field of green prevention and control of crop diseases and insect pests.
Background
The green prevention and control of crop diseases and insect pests is an effective way for promoting the safe production of crops, reducing the use amount of chemical pesticides, adopting environment-friendly measures such as ecological control, biological control, physical control and scientific medication to control the effective behavior of pests, implementing the green prevention and control is a major measure for implementing public plant protection and green plant protection, developing modern agriculture, constructing 'resource saving and environment-friendly' two types of agriculture, and promoting the safety of agricultural production, the quality safety of agricultural products, the agricultural ecological safety and the agricultural trade safety.
Spodoptera litura (Fabricius) is a species of Spodoptera of Lepidoptera, Spodoptera litura, is a crop pest, is brown, has many patches on the front wing, and is named because of the gray and wide twill. The prodenia litura is mainly harmed by larvae, the larvae are edible and are mixed, the food consumption is large, the larvae which are hatched just before are harmed by leaf backs, the leafy flesh is eaten, and only the epidermis is left; after 3-year larvae, the leaves are incised, incomplete and even completely eaten, and the silkworms eat buds to cause defects and easily burst into disasters. At present, the method for preventing and controlling prodenia litura comprises the steps of preventing and controlling by using medicaments, such as spraying imidacloprid, dichlorvos and the like before 3 instars of larvae; trapping and killing adults or utilizing natural enemies of adults such as ladybug and spider for catching and killing.
Coronatine (COR) is a novel plant growth regulating substance formed by coupling a coronaric acid (CMA) containing an amino acid and coronaric acid (CFA) having a polyketone structure by an amide bond. The compound can effectively promote the biosynthesis of plant secondary metabolites, improve the stress resistance of plants, such as drought resistance of corn, peanut, banana and winter wheat during overwintering, cold resistance of wheat and cucumber, salt tolerance of cotton and the like, increase the yield of taxol, soybean flavonoid and the like, improve tobacco quality, have a paddy field weed control effect, and can soak seeds to cultivate strong rice seedlings and promote corn seed germination and the like by using low-concentration coronatine.
Disclosure of Invention
The invention aims to provide a new application of coronatine, namely an application of coronatine in inducing crop seedlings to defend prodenia litura.
Preferably, the crops are pakchoi, cucumber, lettuce, asparagus lettuce, cotton or tobacco.
Preferably, the coronatine is sprayed on the foliage of crops.
Preferably, the spraying concentration of the coronatine is 0.001-1 ppm.
The second technical problem solved by the invention is to provide another new application of coronatine, namely the application of coronatine in preparing resistance inducer.
The coronatine can be applied to the preparation of resistance inducer, and the resistance inducer can induce the defense of crop seedlings, thereby inhibiting the growth and development of spodoptera litura larvae inhabiting the crops.
Preferably, the resistance inducer also comprises a carrier acceptable in pesticide formulation processing.
Preferably, the crops are pakchoi, cucumber, lettuce, asparagus lettuce, cotton or tobacco.
The invention also provides a method for preventing and controlling the prodenia litura of crops.
According to the method for preventing and treating the spodoptera litura of the crops, the coronatine aqueous solution with the concentration of 0.001-1ppm is sprayed on the leaf surfaces of the crops when the seedlings of the crops are planted.
Preferably, the crops are pakchoi, cucumber, lettuce, asparagus lettuce, cotton or tobacco.
Compared with the prior art, the invention has the following beneficial effects:
the method utilizes coronatine to delay the growth and development of spodoptera litura larvae, has the advantages of low consumption, good effect and low environmental pollution, and provides a new choice for green prevention and control of spodoptera litura.
Detailed Description
The invention relates to a new application of coronatine, in particular to an application of coronatine in inducing crop seedlings to defend prodenia litura.
Researches show that the coronatine can induce crop seedling defense, so that the growth and development of spodoptera litura larvae inhabiting the crops are inhibited.
Plant-induced defenses can alter the behavior of herbivores and their natural enemies, and application of exogenous compounds (elicitors) can induce plants to produce this defense response. The inventor finds that the application of coronatine on crops can induce the crop seedlings to generate defense reaction, so that the growth and development of spodoptera litura larvae on the crops are slow, and the aim of preventing and treating the spodoptera litura is fulfilled.
Preferably, the crops are pakchoi, cucumber, lettuce, asparagus lettuce, cotton or tobacco.
Preferably, the coronatine is sprayed on the leaf surfaces of crops, can induce crop defense and inhibit the growth and development of spodoptera litura larvae.
Preferably, the spraying concentration of the coronatine is 0.001-1 ppm.
The method for applying coronatine of the present invention can adopt the conventional application method of pesticides in the field, for example, spraying coronatine solution to the leaf surface of crops, and the leaf surface is fully moistened without dripping water.
The invention provides another new application of coronatine, namely the application of coronatine in preparing an inducer.
The coronatine can be applied to the preparation of resistance inducer, and the resistance inducer can induce the defense of crop seedlings, thereby inhibiting the growth and development of spodoptera litura larvae inhabiting the crops.
Preferably, the resistance inducer also comprises a carrier acceptable in pesticide formulation processing.
The resistance inducer can be prepared into various common dosage forms according to the conventional production method in the field of pesticide formulation processing. For example, coronatine is mixed with one or more carriers, and prepared into aqueous solution, powder, granules and other dosage forms as required.
The carriers acceptable in the processing of pesticide dosage forms refer to carriers acceptable in the field when the pesticide dosage forms are prepared, and the carriers can be configured with coronatine to be conveniently applied to sites to be treated, or can be added into the carriers to be conveniently stored, transported or operated. The carrier may be a pesticide carrier conventional in the field of pesticide formulation processing, for example, a dispersant, wetting agent, binder, emulsifier, and the like.
Preferably, the crops are pakchoi, cucumber, lettuce, asparagus lettuce, cotton or tobacco.
The invention also provides a method for preventing and controlling the prodenia litura of crops.
According to the method for preventing and treating the spodoptera litura of the crops, the coronatine aqueous solution with the concentration of 0.001-1ppm is sprayed on the leaf surfaces of the crops when the seedlings of the crops are planted.
Preferably, the crops are pakchoi, cucumber, lettuce, asparagus lettuce, cotton or tobacco.
The following examples are provided to further illustrate the embodiments of the present invention and are not intended to limit the scope of the present invention.
Example 1
1. Material preparation
Test subjects: little cabbage (Shanghai green)
And (3) sowing the Shanghai green into a seedling raising pot after germination acceleration, uniformly managing water and fertilizer, transplanting the Shanghai green into the seedling raising pot when the Shanghai green grows to 7-8 leaves, and recovering the Shanghai green for testing.
2. Method of administering a drug
Selecting young seedlings with similar growth vigor, dividing into 5 groups (4 medicament groups and 1 control group), spraying coronatine (sprayed to the leaf surface and completely wet, the liquid medicine does not drip down to certain degree) on 20 young seedlings in each group, and spraying equivalent clear water on the control group. After 24h, 1 prodenia litura larva of 1 year is inoculated to each seedling, and the weight of the seedling is recorded.
3. Investigation method
After 10 days of inoculation, the larvae were picked out and investigated, and the growth of the larvae was recorded, and the results are shown in Table 1. The body weights in table 1 are mean body weights.
Growth of spodoptera litura after 110 days in Table
Note: the inhibition rate of the larva growth is [ (weight gain of the larva in the clear water control group-weight gain of the larva in the medicament group)/weight gain of the larva in the clear water control group ]. times.100%
As can be seen from the table 1, spraying coronatine with different concentrations can induce the defense of the Shanghai green seedlings, so that the normal growth of the spodoptera litura larvae is inhibited, the concentration of coronatine is from 0.001 to 1ppm, the growth inhibition effect on the spodoptera litura larvae is gradually enhanced, the effect that the concentration of coronatine is 1ppm is the best, and the growth inhibition rate on the larvae is increased by 1.8 times compared with 0.1 ppm.
Example 2
1. Material preparation
Test subjects: lettuce (glass lettuce)
And (3) sowing the lettuce seeds into a greenhouse field, transplanting the lettuce seeds into a seedling raising pot when 4-5 leaves grow, and performing a test after recovery.
2. Method of administering a drug
Selecting lettuce seedlings with similar growth vigor, dividing into 5 groups (4 medicament groups and 1 control group), spraying coronatine on 20 seedlings in each group, and spraying equivalent clear water on the control group. After 24h, 1 prodenia litura larva of 1 year is inoculated to each seedling, and the weight of the seedling is recorded.
3. Investigation method
After 10 days of inoculation, the larvae were picked out and investigated, and the growth of the larvae was recorded, and the results are shown in Table 2. The body weights in table 2 are mean body weights.
Table 210 days later Prodenia litura larva growth
As can be seen from Table 2, spraying coronatine with different concentrations can induce the defense of lettuce seedlings, so as to inhibit the normal growth of spodoptera litura larvae, the concentration of coronatine is from 0.001 to 1ppm, the growth inhibition effect on the spodoptera litura larvae is gradually enhanced, and the effect of 1ppm of coronatine is the best.
Example 3
1. Material preparation
Test subjects: asparagus lettuce (Baisheng No. 2)
Sowing the asparagus lettuce seeds into a greenhouse, transplanting the asparagus lettuce seeds into a seedling pot when 5-6 leaves grow, and performing test after recovery.
2. Method of administering a drug
The method comprises the steps of selecting asparagus lettuce seedlings with similar growth vigor, dividing the asparagus lettuce seedlings into 5 groups (4 agent groups and 1 control group), spraying coronatine to 20 seedlings in each group, and spraying equivalent clear water to the control groups. After 24h, 1 prodenia litura larva of 1 year is inoculated to each seedling, and the weight of the seedling is recorded.
3. Investigation method
After 10 days of inoculation, the larvae were picked out and investigated, and the growth of the larvae was recorded, and the results are shown in Table 3. The body weights in table 3 are mean body weights.
Growth of spodoptera litura after 310 days in Table
As can be seen from Table 3, spraying coronatine with different concentrations can induce defense of asparagus lettuce seedlings, so that normal growth of spodoptera litura larvae is inhibited, the concentration of coronatine is from 0.001 ppm to 1ppm, the growth inhibition effect on the spodoptera litura larvae is gradually enhanced, and the effect of the coronatine with the concentration of 1ppm on the growth inhibition rate of the larvae is 66.31% is the best.
Example 4
1. Material preparation
Test subjects: cucumber (Nanshui No. 2)
And sowing the asparagus lettuce seeds into a greenhouse, transplanting the asparagus lettuce seeds into a seedling pot when 4 true leaves grow out, and recovering for testing.
2. Method of administering a drug
Selecting cucumber seedlings with similar growth vigor, dividing the cucumber seedlings into 5 groups (4 medicament groups and 1 control group), spraying coronatine on 20 seedlings in each group, and spraying equivalent clear water on the control group. After 24h, 1 prodenia litura larva of 1 year is inoculated to each seedling, and the weight of the seedling is recorded.
3. Investigation method
After 10 days of inoculation, the larvae were picked out and investigated, and the growth of the larvae was recorded, and the results are shown in Table 4. The body weights in table 4 are mean body weights.
Table 410 after the growth of spodoptera litura larvae
As can be seen from Table 4, spraying coronatine with different concentrations can induce cucumber seedling defense, so that the normal growth of spodoptera litura larvae is inhibited, the concentration of coronatine is from 0.001 ppm to 1ppm, the growth inhibition effect on the spodoptera litura larvae is gradually enhanced, the effect that the concentration of coronatine is 1ppm is the best, and the growth inhibition rate of the larvae reaches 70.07%.
Claims (9)
1. Application of coronatine in inducing crop seedling to defend prodenia litura.
2. Use of coronatine according to claim 1 for inducing crop seedlings to defend against prodenia litura, characterized in that: the crops are pakchoi, cucumber, lettuce, asparagus lettuce, cotton or tobacco.
3. Use of coronatine according to claim 1 for inducing crop seedlings to defend against prodenia litura, characterized in that: spraying coronatine on the leaf surface of crops.
4. Use of coronatine according to claim 3 for inducing crop seedlings to defend against prodenia litura, characterized in that: the spraying concentration of coronatine is 0.001-1 ppm.
5. The application of coronatine in preparing an inducer is characterized in that: the resistance inducer can induce crop seedling defense, so as to inhibit the growth and development of spodoptera litura larvae inhabiting the crops.
6. Use of coronatine according to claim 5 for the preparation of a decoy agent characterized in that: the resistance inducer also comprises a carrier acceptable in pesticide formulation processing.
7. Use of coronatine according to claim 5 or 6 for the preparation of a decoy agent characterized in that: the crops are pakchoi, cucumber, lettuce, asparagus lettuce, cotton or tobacco.
8. A method for preventing and controlling crop prodenia litura is characterized by comprising the following steps: when the seedlings of the crops are planted, 0.001-1ppm coronatine aqueous solution is sprayed on the leaf surfaces of the crops.
9. The method for controlling crop prodenia litura according to claim 8, wherein: the crops are pakchoi, cucumber, lettuce, asparagus lettuce, cotton or tobacco.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911065812.3A CN110771620A (en) | 2019-11-04 | 2019-11-04 | Application of coronatine in inducing crop seedlings to defend prodenia litura |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911065812.3A CN110771620A (en) | 2019-11-04 | 2019-11-04 | Application of coronatine in inducing crop seedlings to defend prodenia litura |
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| CN110771620A true CN110771620A (en) | 2020-02-11 |
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| CN201911065812.3A Pending CN110771620A (en) | 2019-11-04 | 2019-11-04 | Application of coronatine in inducing crop seedlings to defend prodenia litura |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003001910A1 (en) * | 2001-06-29 | 2003-01-09 | University Of Florida | Coronatine as an abscission agent |
| WO2008007100A2 (en) * | 2006-07-13 | 2008-01-17 | Plant Bioscience Limited | Improvements in and relating to plant protection |
| CN101107901A (en) * | 2007-02-16 | 2008-01-23 | 江西农业大学 | Application of coronary toxin in restraining seed germination and the simple preprocessing method of coronary toxin biological detecting method thereof |
| CN102239876A (en) * | 2011-05-18 | 2011-11-16 | 中国农业大学 | Application of coronatine as novel cotton defoliant |
| CN107920487A (en) * | 2015-07-02 | 2018-04-17 | 莫迪卡戈公司 | Jasmonic Pathway Activation agent |
| US20180135070A1 (en) * | 2016-10-27 | 2018-05-17 | Board Of Trustees Of Michigan State University | Structurally modified coi1 |
| CN109310083A (en) * | 2016-03-29 | 2019-02-05 | 吉安特科技股份有限公司 | Nanometer formulation for plant |
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2019
- 2019-11-04 CN CN201911065812.3A patent/CN110771620A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003001910A1 (en) * | 2001-06-29 | 2003-01-09 | University Of Florida | Coronatine as an abscission agent |
| WO2008007100A2 (en) * | 2006-07-13 | 2008-01-17 | Plant Bioscience Limited | Improvements in and relating to plant protection |
| CN101107901A (en) * | 2007-02-16 | 2008-01-23 | 江西农业大学 | Application of coronary toxin in restraining seed germination and the simple preprocessing method of coronary toxin biological detecting method thereof |
| CN102239876A (en) * | 2011-05-18 | 2011-11-16 | 中国农业大学 | Application of coronatine as novel cotton defoliant |
| CN107920487A (en) * | 2015-07-02 | 2018-04-17 | 莫迪卡戈公司 | Jasmonic Pathway Activation agent |
| CN109310083A (en) * | 2016-03-29 | 2019-02-05 | 吉安特科技股份有限公司 | Nanometer formulation for plant |
| US20180135070A1 (en) * | 2016-10-27 | 2018-05-17 | Board Of Trustees Of Michigan State University | Structurally modified coi1 |
Non-Patent Citations (3)
| Title |
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| 席征等: "茉莉酸甲酯诱导烟草抗斜纹夜蛾的作用", 《烟草科技》 * |
| 段留生: "《植物激素：合成、信号转导和作用》", 30 June 2008 * |
| 马广民等: "外源茉莉酸甲酯诱导的棉花防御对棉蚜及斜纹夜蛾发育的影响", 《应用昆虫学报》 * |
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| CB02 | Change of applicant information |
Address after: 611630 No. five, No. 35 Industrial Road, Heshan Town, Pujiang County, Sichuan, Chengdu Applicant after: Chengdu new Chaoyang Crop Science Co.,Ltd. Address before: 611630 No. five, No. 35 Industrial Road, Heshan Town, Pujiang County, Sichuan, Chengdu Applicant before: CHENGDU NEWSUN CROPSCIENCE Co.,Ltd. |
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Application publication date: 20200211 |
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| RJ01 | Rejection of invention patent application after publication |