CN110763502A - ROV-based extrusion type in-situ fixing device and fixing method for large organisms such as deep sea mussels - Google Patents
ROV-based extrusion type in-situ fixing device and fixing method for large organisms such as deep sea mussels Download PDFInfo
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- CN110763502A CN110763502A CN201911156910.8A CN201911156910A CN110763502A CN 110763502 A CN110763502 A CN 110763502A CN 201911156910 A CN201911156910 A CN 201911156910A CN 110763502 A CN110763502 A CN 110763502A
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- 238000001125 extrusion Methods 0.000 title claims abstract description 134
- 238000011065 in-situ storage Methods 0.000 title claims abstract description 32
- 241000237536 Mytilus edulis Species 0.000 title claims abstract description 28
- 235000020638 mussel Nutrition 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 18
- 239000003921 oil Substances 0.000 claims abstract description 27
- 238000003860 storage Methods 0.000 claims abstract description 25
- 239000012472 biological sample Substances 0.000 claims abstract description 21
- 239000007788 liquid Substances 0.000 claims abstract description 14
- 238000007789 sealing Methods 0.000 claims abstract description 14
- 238000005070 sampling Methods 0.000 claims abstract description 8
- 239000010720 hydraulic oil Substances 0.000 claims abstract description 4
- 230000033001 locomotion Effects 0.000 claims description 11
- 238000004140 cleaning Methods 0.000 claims description 7
- 230000008569 process Effects 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 230000008602 contraction Effects 0.000 claims description 3
- 230000007797 corrosion Effects 0.000 abstract description 3
- 238000005260 corrosion Methods 0.000 abstract description 3
- 238000003825 pressing Methods 0.000 description 6
- 244000005700 microbiome Species 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 239000000834 fixative Substances 0.000 description 4
- 238000004891 communication Methods 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 2
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- 229910001069 Ti alloy Inorganic materials 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000010485 coping Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000006353 environmental stress Effects 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 229910052755 nonmetal Inorganic materials 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K80/00—Harvesting oysters, mussels, sponges or the like
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Health & Medical Sciences (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to an ROV-based extrusion type in-situ fixing device for large organisms such as deep sea mussels and the like and a fixing method thereof.A squeezing fixing cabin and a driving oil cylinder are respectively arranged on the ROV, the ROV provides hydraulic oil for the driving oil cylinder, the front end and the rear end of the squeezing fixing cabin are respectively connected with a front cover and a rear cover in a sealing threaded manner, a rigid body is inserted into the squeezing fixing cabin and is connected with a piston, and the piston is in sealing sliding connection with the inner wall of the squeezing fixing cabin; an extrusion front plate is arranged on the inner surface of the front cover, an extrusion rear plate is arranged on the surface of one side, facing the front cover, of the piston, and a throwing port for throwing the biological sample into the cabin is formed in the extrusion fixing cabin; the fixed liquid storage bag is internally stored with fixed solution and is communicated with the inside of the extrusion fixing cabin between the extrusion front plate and the extrusion rear plate through a rubber tube channel. The invention meets the in-situ fixation requirement of large-scale organisms such as mussels and the like in a deep sea ecosystem, is slightly influenced by the sampling depth, has strong corrosion resistance, large storage volume and flexible and stable work, and can quickly and effectively fix large-scale organism samples.
Description
Technical Field
The invention belongs to the field of in-situ fixation of large organisms such as deep sea mussels and the like, and particularly relates to an ROV (remote operated vehicle) -based extrusion type in-situ fixation device and a fixation method for the large organisms such as the deep sea mussels and the like.
Background
Because the deep sea environment and the upper ocean environment have huge physicochemical difference, and the sampling is difficult to be completed in a short time by the water depth of thousands of meters, the physiological state of organisms in the sampling process is undoubtedly changed greatly. The current sampling means cannot truly reproduce the real state of deep sea, and is a main bottleneck for restricting the research of deep sea organisms. Deep-sea organisms adaptively evolve under extreme environments to generate a series of special environmental stress coping mechanisms of natural products, and gene resources which need to be developed urgently are stored.
At present, research and development of related fixed equipment are carried out by a plurality of domestic marine research institutions; however, most of the studies on the immobilization technology of deep-sea organisms are mainly conducted by simple storage mechanisms for microorganisms or large-sized organisms, such as: a microorganism in-situ automatic enrichment fixing device, a deep sea extreme environment microorganism primary productivity in-situ detection device and the like. The fixing equipment for large-scale organisms is still in a preliminary research stage, no or few examples of research and application are provided in China, and the difficulty is large-scale organism capturing mode, crushing mode, fixing mode and the like. Moreover, the overall structure of the simple storage mechanism for microorganisms or large-sized organisms is not suitable for ROV (remote operated vehicle) operation, and the biological fixing effect is not good.
Disclosure of Invention
The invention aims to provide an ROV-based extrusion type in-situ fixing device and method for large-scale organisms such as deep sea mussels, which aim to combine the characteristics of the in-situ fixing mode of the large-scale organisms such as the deep sea mussels with the advantages of the traditional in-situ enrichment and fixation of the deep sea microorganisms. The invention utilizes the accurate positioning and capturing capability, the power supply capability and the communication capability of the ROV to greatly reduce the difficulty of the implementation mode.
The purpose of the invention is realized by the following technical scheme:
the extrusion type in-situ fixing device comprises a fixed liquid storage bag, a front cover, an extrusion fixing cabin, a rear cover, a driving oil cylinder, an extrusion front plate, an extrusion rear plate, a rigid body and a piston, wherein the extrusion fixing cabin and the driving oil cylinder are respectively installed on an ROV (remote operated vehicle), the ROV provides hydraulic oil for the driving oil cylinder, the front end and the rear end of the extrusion fixing cabin are respectively connected with the front cover and the rear cover in a sealing and threaded manner, the rigid body output by the driving oil cylinder is inserted into the extrusion fixing cabin and is connected with the piston, and the piston is in sealing sliding connection with the inner wall of the extrusion fixing cabin; an extrusion front plate is arranged on the inner surface of the front cover, an extrusion rear plate linked with the piston is arranged on the surface of one side, facing the front cover, of the piston, and a throwing port for throwing the biological sample into the cabin is formed in the extrusion fixing cabin; the fixed liquid storage bag is internally provided with a fixed solution and is communicated with the interior of the extrusion fixed cabin between the extrusion front plate and the extrusion rear plate through a rubber tube channel.
Wherein: the extrusion surfaces of the extrusion front plate and the extrusion rear plate are inclined planes.
The inclined planes of the extrusion front plate and the extrusion rear plate are parallel.
The driving oil cylinder is provided with a guide disc, at least one guide hole is formed in the guide disc, a guide rod penetrates through the guide hole, one end of the guide rod is inserted into the extrusion fixing cabin and is connected with the piston to limit the rotation of the piston and the driving oil cylinder, and the other end of the guide rod always slides in the guide hole.
The front cover is provided with a pressure relief joint, one end of the pressure relief joint is communicated with the inside of the extrusion fixing cabin between the extrusion front plate and the extrusion rear plate, the other end of the pressure relief joint is communicated with the outside through a pipeline, and the pipeline is provided with a pressure relief valve.
The front cover is provided with a fixed joint, one end of the fixed joint is communicated with the inside of an extrusion fixing cabin between the extrusion front plate and the extrusion rear plate, the other end of the fixed joint is communicated with a fixed liquid storage bag through the rubber pipe channel, and the rubber pipe channel is provided with a fixed valve.
And a horn mouth convenient for putting the biological sample is arranged on the outer edge of the putting opening.
The length of the driving oil cylinder is equal to the moving distance of the rigid body in the extrusion fixing cabin.
The invention relates to a fixing method of an ROV-based extrusion type in-situ fixing device for large organisms such as deep sea mussels, which comprises the following steps: the method comprises the following steps:
firstly, disassembling and cleaning the whole in-situ fixing device at the shore base end;
step two, assembling after cleaning, wherein the fixing solution is filled in the fixing solution storage bag in an initial state, the piston is abutted against the rear cover, and a fixing valve arranged on a rubber tube channel between the inside of the extrusion fixing cabin and the fixing solution storage bag and a pressure relief valve arranged on a communication pipeline between the inside of the extrusion fixing cabin and the outside are both in a closed state;
step three, throwing a biological sample into the extrusion fixed cabin through the throwing port, opening the pressure relief valve, starting the driving oil cylinder to enable the rigid body to extend and move, driving the extrusion rear plate to extend and move forwards through the piston, and pushing the biological sample to be contacted with the extrusion front plate by the extrusion rear plate to finish the extrusion process; the water body in the extrusion fixing cabin is discharged through the pressure relief valve, the pressure relief valve is closed, the fixing valve is opened, the driving oil cylinder is started in the opposite direction, the rigid body is contracted to move, the extrusion rear plate is driven to retreat through the piston, suction is formed in the extrusion fixing cabin, the fixing solution in the fixing solution storage bag is sucked, and the biological sample fixing work is completed;
step four, sampling at the shore base end; and (4) recovering the in-situ fixing device to the shore base end, dismantling the front cover, and taking out large organisms such as mussels to complete the whole operation.
Wherein: and in the rigid body contraction motion, the motion condition of the piston is observed, so that a space is reserved between the piston and the front end of the throwing port, and the sealing O ring is utilized to ensure the tightness of the extrusion fixing cabin in the whole motion process.
The invention has the advantages and positive effects that:
1. the invention meets the in-situ fixation requirement of large-scale organisms such as mussels and the like in a deep sea ecosystem, is slightly influenced by the sampling depth, has strong corrosion resistance, large storage volume and flexible and stable work, and can quickly and effectively fix large-scale organism samples.
2. The invention is based on an ROV system, and can reuse the characteristics of the ROV, such as accurate positioning, real-time communication, effective controllability and the like.
3. The invention has small and compact structure, stable operation and small error, can be suitable for complex seabed environment under various depth, temperature and ocean current environments, and can be widely applied to acquisition and fixation of large organisms such as mussels and the like needing strict fidelity.
Drawings
FIG. 1 is a schematic view of the overall structure of the extrusion type in-situ fixing device of the invention;
wherein: the device comprises a fixed liquid storage bag 1, a rubber tube channel 2, a fixed valve 3, a fixed joint 4, a pressure relief valve 5, a pressure relief joint 6, a front cover 7, an extrusion fixed cabin 8, a horn mouth 9, a placing opening 10, a rear cover 11, a guide rod 12, a bolt 13, a guide hole 14, a driving oil cylinder 15, an oil port 16, an extrusion front plate 17, an extrusion rear plate 18, a rigid body 19, a guide disc 20, a piston 21 and a sealing O ring 22.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
As shown in fig. 1, the extrusion type in-situ fixing device of the present invention includes a fixed liquid storage bag 1, a front cover 7, an extrusion fixing chamber 8, a rear cover 11, a driving cylinder 15, an extrusion front plate 17, an extrusion rear plate 18, a rigid body 19 and a piston 21, wherein the extrusion fixing chamber 8 and the driving cylinder 15 are respectively fixedly mounted with a bottom plate of an ROV by using a fixing clip, and the ROV uses an oil port 16 on the driving cylinder 15 to provide hydraulic oil for the driving cylinder 15 to complete the reciprocating motion of the rigid body 19. A fixative solution is stored in the fixative solution storing bag 1, and the fixative solution in this embodiment is a fixative solution RNAlater.
The front end and the rear end of the extrusion fixing cabin 8 are respectively connected with a front cover 7 and a rear cover 11 in a sealing threaded manner, a rigid body 19 (namely a piston rod of the driving oil cylinder 15) output by the driving oil cylinder 15 is inserted into the extrusion fixing cabin 8 through the rear cover 11 and is connected with a piston 21, a sealing O ring 22 is arranged on the outer ring of the piston 21, and the piston 21 is in sealing sliding connection with the inner wall of the extrusion fixing cabin 8; in the embodiment, a rear extrusion plate 18 is fixedly connected to one side surface of the piston 21 facing the front cover 7, and the other side surface of the piston 21 facing the rear cover 11 is fixedly connected to the rigid body 19 through two bolts 13; an extrusion front plate 17 is fixedly connected with the inner surface of the front cover 7. In this embodiment, the pressing surfaces of the pressing front plate 17 and the pressing rear plate 18 are both inclined surfaces (inclined angle is 60 °), and the inclined surfaces of the pressing front plate 17 and the pressing rear plate 18 are parallel to each other, thereby pressing a large biological sample. The length of the driving oil cylinder 15 is equal to the moving distance of the rigid body 19 in the extrusion fixing chamber 8, and the driving oil cylinder is used for ensuring that the extrusion crushing distance of the biological sample in the extrusion fixing chamber 8 and the volume of the extracted fixing solution are realized in a set mode.
During the reciprocating motion of the rigid body 19 under the independent operation of the driving cylinder 15, the piston 21 is easy to rotate. In order to avoid the rotation of the piston 21 and the driving cylinder 15, a guide disc 20 is installed on the driving cylinder 15, at least one guide hole 14 (two guide holes 14 in this embodiment) is opened on the guide disc 20, a guide rod 12 penetrates through the guide hole 14, one end of the guide rod 12 is inserted into the extrusion fixing cabin 8 through the rear cover 11 and is connected with the piston 13 to limit the rotation of the piston 13 and the driving cylinder 15, and the other end of the guide rod 12 freely slides in the guide hole 14 all the time.
The extrusion fixing cabin 8 is provided with a throwing port 10 for throwing the biological samples into the cabin, and the opening diameter of the throwing port 10 can be adjusted according to the external dimensions of large organisms such as deep sea mussels and the like; in order to put the biological sample into the putting-in opening 10, the bell mouth 9 for putting the biological sample is arranged at the outer edge of the putting-in opening 10.
The front cover 7 is respectively provided with a fixed joint 4 and a pressure relief joint 6, one end of the fixed joint 4 is communicated with the inside of an extrusion fixed cabin 8 between an extrusion front plate 17 and an extrusion rear plate 18, the other end is communicated with a fixed liquid storage bag 1 through a rubber tube channel 2, and the rubber tube channel 2 is provided with a fixed valve 3. One end of the pressure relief joint 6 is communicated with the inside of the extrusion fixing cabin 8 between the extrusion front plate 17 and the extrusion rear plate 18, the other end is communicated with the outside through a pipeline, and a pressure relief valve 5 is arranged on the pipeline. The fixed liquid storage bag 1, the rubber tube channel 2, the fixed valve 3, the fixed joint 4 and the front cover 7 form a passage.
The hose passage 2 of the present embodiment is manufactured by saint-gobain, france. The extrusion type in-situ fixing device is integrally made of a non-metal transparent material (such as Teflon), and the fixing valve 3, the pressure relief valve 5 and the horn mouth 9 are made of metal materials (Ti alloy) so as to avoid sample pollution.
The extrusion type in-situ fixing device of the invention needs a fixing method for obtaining, crushing and fixing large organisms such as deep sea mussels and the like, and comprises the following steps:
firstly, integrally disassembling and cleaning a shore-based end, integrally disassembling and disassembling large-scale organisms in-situ fixing devices such as deep sea mussels and the like, and then cleaning;
step two, assembling after cleaning, wherein the fixed liquid storage bag 1 is filled with a fixed solution in an initial state, the piston 21 is abutted against the rear cover 11, and the fixed valve 3 on the rubber tube channel 2 between the inside of the extrusion fixing cabin 8 and the fixed liquid storage bag 1 and the pressure relief valve 5 on the pipeline communicated with the outside inside of the extrusion fixing cabin 8 are both in a closed state;
thirdly, observing and grabbing large biological samples (such as mussels) through ROV deep sea high-definition camera shooting, putting the large biological samples into a bell mouth 9, enabling the biological samples to fall into an extrusion fixed cabin 8 through the action of gravity, opening a pressure relief valve 5, starting a driving oil cylinder 15, enabling a rigid body 19 to stretch out and move, driving an extrusion rear plate 18 to stretch forward through a piston 21, and enabling the extrusion rear plate 18 to push the large biological samples to be in contact with an extrusion front plate 17 to finish the extrusion process; discharging water in the extrusion fixing cabin 8 through a pressure relief valve 5, closing the pressure relief valve 5 by using an ROV manipulator, opening a fixing valve 3, starting a driving oil cylinder 15 in the opposite direction to enable a rigid body 19 to contract and move, driving an extrusion rear plate 18 to retreat through a piston 21, enabling the interior of the extrusion fixing cabin 8 to form suction, sucking a fixing solution in a fixing solution storage bag 1, and finishing the fixing work of a biological sample; in the contraction movement of the rigid body 19, ROV deep sea high definition camera shooting is used for observing the movement condition of the piston 21, so that a space is reserved between the piston 21 and the front end of the throwing port 10 (namely the rear edge of the piston 33 does not contact the front end of the throwing port 10), and in the whole movement process, a sealing O ring 22 is used for ensuring the tightness of the extrusion fixing cabin 8;
step four, sampling at the shore base end; and (4) recovering the in-situ fixing device to the shore base end, dismantling the front cover 7, and taking out large organisms such as mussels to complete the whole operation.
The invention has small and compact structure, strong corrosion resistance and small running error of stable rotation, can be suitable for complex seabed environment under various depth, temperature and ocean current environments, and can be widely applied to acquisition and fixation of large organisms such as mussels and the like needing strict fidelity. The invention meets the fixation requirement of large-scale organisms, can be used in the environment with complicated and variable deep sea pressure, and provides support for various physiological and biochemical researches on the large-scale organisms in the deep sea.
Claims (10)
1. The utility model provides a deep sea mussel etc. macrobiosis extrusion formula normal position fixing device based on ROV which characterized in that: the device comprises a fixed liquid storage bag (1), a front cover (7), an extrusion fixing cabin (8), a rear cover (11), a driving oil cylinder (15), an extrusion front plate (17), an extrusion rear plate (18), a rigid body (19) and a piston (21), wherein the extrusion fixing cabin (8) and the driving oil cylinder (15) are respectively installed on an ROV (remote operated vehicle), the ROV provides hydraulic oil for the driving oil cylinder (15), the front end and the rear end of the extrusion fixing cabin (8) are respectively connected with the front cover (7) and the rear cover (11) in a sealing threaded manner, the rigid body (19) output by the driving oil cylinder (15) is inserted into the extrusion fixing cabin (8) and is connected with the piston (21), and the piston (21) is connected with the inner wall of the extrusion fixing cabin (8) in a sealing sliding manner; an extrusion front plate (17) is arranged on the inner surface of the front cover (7), an extrusion rear plate (18) linked with the piston (21) is arranged on the surface of one side, facing the front cover (7), of the piston (21), and a throwing port (10) for throwing the biological sample into the cabin is formed in the extrusion fixing cabin (8); the fixed solution is stored in the fixed solution storage bag (1), and the fixed solution storage bag (1) is communicated with the interior of the extrusion fixed cabin (8) between the extrusion front plate (17) and the extrusion rear plate (18) through the rubber tube channel (2).
2. The ROV-based extrusion type in-situ fixing device for large organisms such as deep sea mussels is characterized in that: the extrusion surfaces of the extrusion front plate (17) and the extrusion rear plate (18) are inclined surfaces.
3. The ROV-based extrusion type in-situ fixing device for large organisms such as deep sea mussels is characterized in that: the inclined surfaces of the extrusion front plate (17) and the extrusion rear plate (18) are parallel.
4. The ROV-based extrusion type in-situ fixing device for large organisms such as deep sea mussels is characterized in that: install guiding disc (20) on actuating cylinder (15), seted up at least one guiding hole (14) on this guiding disc (20), wear to be equipped with guide bar (12) in guiding hole (14), the one end of this guide bar (12) is inserted in the fixed cabin of extrusion (8), and with piston (13) link to each other, restriction piston (13) and actuating cylinder (15) rotate, and the other end of guide bar (12) is in all the time slide in guiding hole (14).
5. The ROV-based extrusion type in-situ fixing device for large organisms such as deep sea mussels is characterized in that: the pressure relief joint (6) is installed on the front cover (7), one end of the pressure relief joint (6) is communicated with the inside of the extrusion fixing cabin (8) between the extrusion front plate (17) and the extrusion rear plate (18), the other end of the pressure relief joint is communicated with the outside through a pipeline, and a pressure relief valve (5) is installed on the pipeline.
6. The ROV-based extrusion type in-situ fixing device for large organisms such as deep sea mussels is characterized in that: the front cover (7) is provided with a fixed joint (4), one end of the fixed joint (4) is communicated with the inside of an extrusion fixed cabin (8) between the extrusion front plate (17) and the extrusion rear plate (18), the other end of the fixed joint is communicated with the fixed liquid storage bag (1) through the rubber pipe channel (2), and the rubber pipe channel (2) is provided with a fixed valve (3).
7. The ROV-based extrusion type in-situ fixing device for large organisms such as deep sea mussels is characterized in that: and a bell mouth (9) convenient for throwing the biological sample is arranged on the outer edge of the throwing port (10).
8. The ROV-based extrusion type in-situ fixing device for large organisms such as deep sea mussels is characterized in that: the length of the driving oil cylinder (15) is equal to the moving distance of the rigid body (19) in the extrusion fixed cabin (8).
9. A fixing method of a ROV-based large-scale organism extrusion type in-situ fixing device of deep sea mussels and the like according to any one of claims 1 to 8, which is characterized in that: the method comprises the following steps:
firstly, disassembling and cleaning the whole in-situ fixing device at the shore base end;
step two, assembling after cleaning, wherein the fixed liquid storage bag (1) is filled with a fixed solution in an initial state, the piston (21) is abutted against the rear cover (11), and a fixed valve (3) arranged on a rubber tube channel (2) between the inside of the extrusion fixing cabin (8) and the fixed liquid storage bag (1) and a pressure relief valve (5) arranged on a pipeline communicated with the outside inside of the extrusion fixing cabin (8) are both in a closed state;
thirdly, throwing a biological sample into the extrusion fixed cabin (8) through the throwing port (10), opening the pressure relief valve (5), starting the driving oil cylinder (15) to enable the rigid body (19) to extend and move, driving the extrusion rear plate (18) to extend and move through the piston (21), and pushing the biological sample to be contacted with the extrusion front plate (17) through the extrusion rear plate (18) to finish the extrusion process; after the water in the extrusion fixing cabin (8) is discharged through the pressure relief valve (5), the pressure relief valve (5) is closed, the fixing valve (3) is opened, the driving oil cylinder (15) is started in the opposite direction, the rigid body (19) contracts and moves, the extrusion rear plate (18) is driven to retreat through the piston (21), so that suction is formed inside the extrusion fixing cabin (8), the fixing solution in the fixing solution storage bag (1) is sucked, and the biological sample fixing work is completed;
step four, sampling at the shore base end; and (4) recovering the in-situ fixing device to the shore base end, dismantling the front cover (7), and taking out large organisms such as mussels to complete the whole operation.
10. The method of securing according to claim 9, wherein: during the contraction movement of the rigid body (19), the movement condition of the piston (21) is observed, so that a distance is reserved between the piston (21) and the front end of the throwing opening (10), and the sealing performance of the extrusion fixing cabin (8) is guaranteed by using a sealing O ring (22) in the whole movement process.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911156910.8A CN110763502A (en) | 2019-11-22 | 2019-11-22 | ROV-based extrusion type in-situ fixing device and fixing method for large organisms such as deep sea mussels |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911156910.8A CN110763502A (en) | 2019-11-22 | 2019-11-22 | ROV-based extrusion type in-situ fixing device and fixing method for large organisms such as deep sea mussels |
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| CN110763502A true CN110763502A (en) | 2020-02-07 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112219780A (en) * | 2020-10-20 | 2021-01-15 | 中国科学院海洋研究所 | Long-time-series deep-sea in-situ large-scale organism stress culture device and use method thereof |
| CN112616757A (en) * | 2020-12-25 | 2021-04-09 | 中国科学院海洋研究所 | ROV-based deep sea in-situ large-scale organism culture device and use method thereof |
-
2019
- 2019-11-22 CN CN201911156910.8A patent/CN110763502A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112219780A (en) * | 2020-10-20 | 2021-01-15 | 中国科学院海洋研究所 | Long-time-series deep-sea in-situ large-scale organism stress culture device and use method thereof |
| CN112219780B (en) * | 2020-10-20 | 2023-09-12 | 中国科学院海洋研究所 | Long-time-sequence deep-sea in-situ large-scale biological stress culture device and use method thereof |
| CN112616757A (en) * | 2020-12-25 | 2021-04-09 | 中国科学院海洋研究所 | ROV-based deep sea in-situ large-scale organism culture device and use method thereof |
| CN112616757B (en) * | 2020-12-25 | 2023-07-25 | 中国科学院海洋研究所 | ROV-based deep sea in-situ large-scale biological culture device and use method thereof |
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