CN110747119A - Centrifugal collection device suitable for stem cell constant temperature is airtight - Google Patents
Centrifugal collection device suitable for stem cell constant temperature is airtight Download PDFInfo
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
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Abstract
The invention discloses a centrifugal collection device suitable for stem cells to be sealed at constant temperature. The centrifugal collection device is in contact extrusion with a stem cell containing container through a first contact plate, a cover body is placed on a funnel-shaped main body, the cover body is tightly fastened and sealed through a fastener, a first door plate is closed, a motor drives a centrifugal piece to rotate, constant-temperature centrifugation on stem cells is achieved, collection is carried out, a support piece is adopted to carry out damping and buffering on the stem cell containing container in the vertical direction, normal centrifugation on the stem cells is guaranteed, and pollution and orderly proceeding of experiments are avoided; the temperature sensor transmits the temperature to the controller, the electromagnetic valve is adjusted through the controller, the centrifugal temperature is controlled, stem cells are prevented from dying due to the temperature in the centrifugal process, and the centrifugal quality is improved.
Description
Technical Field
The invention relates to a centrifugal collecting device, in particular to a centrifugal collecting device suitable for stem cells to be sealed at constant temperature.
Background
The stem cells in the human body are divided into two types, one type is full-function stem cells, and the stem cells can be directly cloned into the human body; the other is a multifunctional stem cell which can directly copy various organs and repair tissues. It is hoped that the isolation and in vitro culture of stem cells are utilized to breed tissues or organs in vitro, and finally, the treatment of clinical diseases is realized through tissue or organ transplantation. The new skin regenerating technology by in-situ cultured skin stem cell not only realizes the reproduction of skin organ by stem cell, but also realizes the reproduction of in-situ skin organ of human body, thus leading human to cultivate tissue organ transplantation treatment from stem cell in vitro, and directly striding into the in-situ stem cell reproduced organ of human body. Scientists generally believe that: the research of the stem cells can provide wider application prospect for clinical medicine.
At present, stem cells are separated on a vertical square, equipment vibrates, breakage of a container is easily caused, stem cells are polluted, meanwhile, temperature guarantee does not exist during centrifugation, stem cells are easily killed, and the centrifugation quality is low.
Disclosure of Invention
The invention aims to provide a centrifugal collecting device suitable for constant-temperature sealing of stem cells, wherein a temperature control pipe body is opened to enable the temperature of a first placing area to reach the constant temperature for separating the stem cells, a container containing the stem cells is placed in a placing hole, a supporting piece is placed on a supporting column, the supporting piece is in contact extrusion with the stem cell containing container through a first contact plate, a cover body is placed on a funnel-shaped main body, the cover body is fastened and sealed through a fastener, a first door plate is closed, a motor drives a centrifugal piece to rotate, constant-temperature centrifugation and collection of the stem cells are achieved, the supporting piece is adopted to perform damping and buffering on the stem cell containing container in the vertical direction, normal centrifugation of the stem cells is ensured, and pollution and orderly proceeding of experiments are avoided; the temperature sensor transmits the temperature to the controller, the electromagnetic valve is adjusted through the controller, the centrifugal temperature is controlled, stem cells are prevented from dying due to the temperature in the centrifugal process, and the centrifugal quality is improved.
The purpose of the invention can be realized by the following technical scheme:
the utility model provides a be applicable to airtight centrifugal collection system of stem cell constant temperature, centrifugal collection system includes the cabinet body, the cabinet body is equipped with the baffle including the cabinet main part in the middle of the cabinet main part, and the baffle is cut apart into the cabinet main part and is first place the district and place the district with the second.
The partition plate is provided with a first through hole, and the cabinet main body is provided with a second through hole and a third through hole.
First place the district and rotate respectively in the second place the district and be equipped with first door plant and second door plant, the below of cabinet main part is equipped with the universal wheel of array distribution, is equipped with the control by temperature change body in the first district of placing, and the control by temperature change body includes S type body, and S type body includes first connector and third connector, and the circulation has water in the body, is equipped with the solenoid valve on the control by temperature change body.
A temperature sensor is arranged in the first placing area, and a controller is arranged on one side of the cabinet main body.
The lower part of the partition plate is fixedly connected with a motor, an output shaft of the motor penetrates through the first through hole to be fixedly connected with a centrifugal piece, the centrifugal piece comprises a funnel-shaped main body, and a connecting shaft is arranged below the funnel-shaped main body.
The handles are symmetrically distributed on the funnel-shaped main body, the fasteners are distributed in an array mode on the funnel-shaped main body, and the cover body is arranged on the funnel-shaped main body.
The funnel-shaped main part is internally provided with a placing table, a supporting sleeve which is distributed in an array manner is arranged on the placing table, the supporting sleeve is provided with placing holes, and the inner wall of the funnel-shaped main part is provided with symmetrically distributed supporting columns.
Be equipped with support piece in the centrifugation piece, support piece includes circular backup pad, and one side of circular backup pad is equipped with the first elastic component that the array distributes, and the opposite side is equipped with the second elastic component that the array distributes, and the one end and the circular backup pad fastening connection of first elastic component, the other end are equipped with first contact plate, the one end and the circular backup pad fastening connection of second elastic component, and the other end is equipped with the second contact plate.
Further, the first through hole is communicated with the first placing area and the second placing area.
Further, the second through hole and the third through hole are located in the first placement area.
Furthermore, the first connecting port and the third connecting port are respectively positioned in the second through hole and the third through hole.
Furthermore, the solenoid valves on the temperature sensor and the temperature control pipe body are electrically connected with the controller, the temperature sensor transmits the temperature to the controller, and the solenoid valves are adjusted through the controller.
Further, the centrifugal piece is fixedly connected with an output shaft of the motor through a connecting shaft.
Further, the cover body is placed on the funnel-shaped main body and is fastened and sealed through a fastener.
Further, the support member is supported on the support column through a circular support plate, and is in contact with the stem cell container through a first contact plate and is in contact with the cover body through a second contact plate.
The invention has the beneficial effects that:
1. the centrifugal collection device is in contact extrusion with a stem cell containing container through a first contact plate, a cover body is placed on a funnel-shaped main body, the cover body is tightly fastened and sealed through a fastener, a first door plate is closed, a motor drives a centrifugal piece to rotate, constant-temperature centrifugation on stem cells is achieved, collection is carried out, a support piece is adopted to carry out damping and buffering on the stem cell containing container in the vertical direction, normal centrifugation on the stem cells is guaranteed, and pollution and orderly proceeding of experiments are avoided;
2. the centrifugal collecting device transmits the temperature to the controller through the temperature sensor, and the controller adjusts the electromagnetic valve to realize the control of the centrifugal temperature, reduce the death of stem cells caused by the temperature in the centrifugal process and improve the centrifugal quality.
Drawings
The invention will be further described with reference to the accompanying drawings.
FIG. 1 is a schematic view of the structure of the centrifugal collection device of the present invention;
FIG. 2 is a schematic view of the centrifugal collection device of the present invention;
FIG. 3 is a schematic structural view of the cabinet of the present invention;
FIG. 4 is a schematic view of a temperature control tube according to the present invention;
FIG. 5 is a schematic view of the centrifuge construction of the present invention;
FIG. 6 is a partial schematic view of the centrifuge of the present invention;
fig. 7 is a schematic view of the support structure of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The utility model provides a centrifugal collection system suitable for stem cell is airtight at constant temperature, centrifugal collection system includes the cabinet body 1, as shown in fig. 1, fig. 2, fig. 3, cabinet body 1 includes cabinet main part 11, is equipped with baffle 12 in the middle of cabinet main part 11, and baffle 12 is divided into first district 13 and the district 14 is placed to the second with cabinet main part 11.
The partition board 12 is provided with a first through hole 15, the first through hole 15 is communicated with the first placing area 13 and the second placing area 14, the cabinet body 11 is provided with a second through hole 16 and a third through hole 17, and the second through hole 16 and the third through hole 17 are positioned in the first placing area 13.
First place and rotate respectively in district 13 and the second place district 14 and be equipped with first door plant 2 and second door plant 3, the below of cabinet main part 11 is equipped with array distribution' S universal wheel 4, be equipped with control by temperature change body 5 in the first district 13 of placing, as shown in fig. 4, control by temperature change body 5 includes S type body 51, S type body 51 includes first connector 52 and third connector 53, first connector 52 and third connector 53 are located second through hole 16 and third through hole 17 respectively, the circulation has water in the body 51, be equipped with the solenoid valve on the control by temperature change body 5.
First place and be equipped with temperature-sensing ware 6 in the district 13, one side of cabinet main part 11 is equipped with controller 7, control by temperature change body 5 is used for first placing and provides heat energy in the district 13, the first temperature of placing in the district 13 of temperature-sensing ware 6 response, temperature-sensing ware 6, the last solenoid valve of control by temperature change body 5 all with controller 7 electric connection, controller 7 is given with the temperature transmission to temperature-sensing ware 6, adjust the solenoid valve through controller 7, realize the control of temperature.
The motor 8 is tightly connected below the partition plate 12, the centrifugal part 9 is tightly connected to an output shaft of the motor 8 through the first through hole 15, as shown in fig. 5 and 6, the centrifugal part 9 includes a funnel-shaped main body 91, a connecting shaft 92 is arranged below the funnel-shaped main body 91, and the centrifugal part 9 is tightly connected to the output shaft of the motor 8 through the connecting shaft 92.
Be equipped with the handle 93 of symmetric distribution on the infundibulate main part 91, be equipped with the fastener 94 of array distribution on the infundibulate main part 91, be equipped with lid 95 on the infundibulate main part 91, lid 95 is placed on the infundibulate main part 91, and is sealed through the fastening of fastener 94.
The funnel-shaped main body 91 is internally provided with a placing table 96, the placing table 96 is provided with a supporting sleeve 97 distributed in an array manner, the supporting sleeve 97 is provided with a placing hole 971, and the inner wall of the funnel-shaped main body 91 is provided with supporting columns 98 distributed symmetrically.
As shown in fig. 7, the supporting member 10 is disposed in the centrifugal member 9, and the supporting member 10 includes a circular supporting plate 101, one side of the circular supporting plate 101 is provided with first elastic members 102 distributed in an array, the other side of the circular supporting plate 101 is provided with second elastic members 104 distributed in an array, one end of each first elastic member 102 is fastened to the circular supporting plate 101, the other end of each first elastic member 102 is provided with a first contact plate 103, one end of each second elastic member 104 is fastened to the circular supporting plate 101, the other end of each second elastic member is provided with a second contact plate 105, the supporting member 10 is supported on the supporting column 98 by the circular supporting plate 101, is in contact with the stem cell container by the first contact plate 103.
During the use, open control by temperature change body 5 for the first constant temperature who places district 13 reaches the stem cell separation, place the container that contains the stem cell in placing hole 971, place support piece 10 on support column 98, contact the extrusion through first contact plate 103 and stem cell splendid attire container, place lid 95 on infundibulate main part 91, it is sealed to fasten through fastener 94, close first door plant 2, motor 8 drive centrifugation piece 9 takes place to rotate, the realization is to the constant temperature centrifugation of stem cell, gather again.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed.
Claims (8)
1. A centrifugal collection device suitable for stem cells to be sealed at constant temperature comprises a cabinet body (1), and is characterized in that the cabinet body (1) comprises a cabinet main body (11), a partition plate (12) is arranged in the middle of the cabinet main body (11), and the cabinet main body (11) is divided into a first placing area (13) and a second placing area (14) by the partition plate (12);
a first through hole (15) is formed in the partition plate (12), and a second through hole (16) and a third through hole (17) are formed in the cabinet main body (11);
the cabinet is characterized in that a first door plate (2) and a second door plate (3) are respectively arranged in the first placing area (13) and the second placing area (14) in a rotating mode, universal wheels (4) distributed in an array mode are arranged below the cabinet main body (11), a temperature control tube body (5) is arranged in the first placing area (13), the temperature control tube body (5) comprises an S-shaped tube body (51), the S-shaped tube body (51) comprises a first connecting port (52) and a third connecting port (53), water flows through the tube body (51), and an electromagnetic valve is arranged on the temperature control tube body (5);
a temperature sensor (6) is arranged in the first placing area (13), and a controller (7) is arranged on one side of the cabinet main body (11);
a motor (8) is fixedly connected below the partition plate (12), an output shaft of the motor (8) penetrates through the first through hole (15) to be fixedly connected with a centrifugal piece (9), the centrifugal piece (9) comprises a funnel-shaped main body (91), and a connecting shaft (92) is arranged below the funnel-shaped main body (91);
handles (93) which are symmetrically distributed are arranged on the funnel-shaped main body (91), fasteners (94) which are distributed in an array manner are arranged on the funnel-shaped main body (91), and a cover body (95) is arranged on the funnel-shaped main body (91);
a placing table (96) is arranged in the funnel-shaped main body (91), supporting sleeves (97) distributed in an array manner are arranged on the placing table (96), placing holes (971) are formed in the supporting sleeves (97), and supporting columns (98) distributed symmetrically are arranged on the inner wall of the funnel-shaped main body (91);
the centrifugal part is characterized in that a supporting part (10) is arranged in the centrifugal part (9), the supporting part (10) comprises a circular supporting plate (101), first elastic parts (102) distributed in an array mode are arranged on one side of the circular supporting plate (101), second elastic parts (104) distributed in an array mode are arranged on the other side of the circular supporting plate (101), one end of each first elastic part (102) is fixedly connected with the circular supporting plate (101), a first contact plate (103) is arranged at the other end of each first elastic part, one end of each second elastic part (104) is fixedly connected with the circular supporting plate (101), and a second contact plate (105) is.
2. The centrifugal collection device for stem cells closed at constant temperature according to claim 1, wherein the first through hole (15) is communicated with the first placement area (13) and the second placement area (14).
3. The centrifugal collection device suitable for stem cell thermostatic sealing according to claim 1, wherein the second through hole (16) and the third through hole (17) are located in the first placement area (13).
4. The centrifugal collection device suitable for stem cell thermostatic sealing according to claim 1, wherein the first connection port (52) and the third connection port (53) are respectively located in the second through hole (16) and the third through hole (17).
5. The centrifugal collection device suitable for stem cell constant-temperature sealing is characterized in that the temperature sensor (6) and the electromagnetic valve on the temperature control tube body (5) are electrically connected with the controller (7), the temperature sensor (6) transmits the temperature to the controller (7), and the electromagnetic valve is adjusted by the controller (7).
6. The centrifugal collection device suitable for stem cell thermostatic sealing according to claim 1, characterized in that the centrifugal piece (9) is tightly connected with the output shaft of the motor (8) through a connecting shaft (92).
7. The centrifugal collection device for stem cells with constant temperature tightness according to claim 1, wherein the cover body (95) is placed on the funnel-shaped main body (91) and is tightly sealed by a fastener (94).
8. The centrifugal collection device for stem cell thermostatic sealing according to claim 1, wherein the support member (10) is supported on the support column (98) by a circular support plate (101), and is in contact with the stem cell container by a first contact plate (103) and is in contact with the cover (95) by a second contact plate (105).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911207154.7A CN110747119A (en) | 2019-11-29 | 2019-11-29 | Centrifugal collection device suitable for stem cell constant temperature is airtight |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911207154.7A CN110747119A (en) | 2019-11-29 | 2019-11-29 | Centrifugal collection device suitable for stem cell constant temperature is airtight |
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| CN110747119A true CN110747119A (en) | 2020-02-04 |
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| CN201911207154.7A Pending CN110747119A (en) | 2019-11-29 | 2019-11-29 | Centrifugal collection device suitable for stem cell constant temperature is airtight |
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Citations (6)
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| CN107475111A (en) * | 2017-09-26 | 2017-12-15 | 上海莱威生物科技有限公司 | A kind of highly effective pretreatment apparatus and its related application for being used to cultivate human umbilical cord mesenchymal stem cells |
| CN207130267U (en) * | 2017-08-31 | 2018-03-23 | 重庆金时代生物技术有限公司 | A kind of stem cell collection device |
| CN108285861A (en) * | 2018-04-12 | 2018-07-17 | 马鞍山大椿生命科技有限公司 | A kind of stem cell collection device and its application method |
| CN208146230U (en) * | 2018-01-30 | 2018-11-27 | 卡替（上海）生物技术股份有限公司 | A kind of deciduous teeth dental pulp stem cell ambient temperature centrifuge |
| WO2019195242A1 (en) * | 2018-04-02 | 2019-10-10 | Jointechlabs, Inc. | System and methods for preparation of adipose-derived stem cells |
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2019
- 2019-11-29 CN CN201911207154.7A patent/CN110747119A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014112794A1 (en) * | 2013-01-17 | 2014-07-24 | 주식회사 휴림바이오셀 | Device for extracting regenerative cells, system for extracting regenerative cells, and method for extracting regenerative cells using same |
| CN207130267U (en) * | 2017-08-31 | 2018-03-23 | 重庆金时代生物技术有限公司 | A kind of stem cell collection device |
| CN107475111A (en) * | 2017-09-26 | 2017-12-15 | 上海莱威生物科技有限公司 | A kind of highly effective pretreatment apparatus and its related application for being used to cultivate human umbilical cord mesenchymal stem cells |
| CN208146230U (en) * | 2018-01-30 | 2018-11-27 | 卡替（上海）生物技术股份有限公司 | A kind of deciduous teeth dental pulp stem cell ambient temperature centrifuge |
| WO2019195242A1 (en) * | 2018-04-02 | 2019-10-10 | Jointechlabs, Inc. | System and methods for preparation of adipose-derived stem cells |
| CN108285861A (en) * | 2018-04-12 | 2018-07-17 | 马鞍山大椿生命科技有限公司 | A kind of stem cell collection device and its application method |
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