CN110736656B - Phytoplankton concentration device and method - Google Patents
Phytoplankton concentration device and method Download PDFInfo
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- CN110736656B CN110736656B CN201910876421.3A CN201910876421A CN110736656B CN 110736656 B CN110736656 B CN 110736656B CN 201910876421 A CN201910876421 A CN 201910876421A CN 110736656 B CN110736656 B CN 110736656B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
- G01N2001/4083—Concentrating samples by other techniques involving separation of suspended solids sedimentation
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Abstract
The invention discloses a phytoplankton concentration device and method, and aims to overcome the defects that in the phytoplankton concentration process, a fixing solution and a phytoplankton water sample are not uniformly mixed, the phytoplankton is easily taken away in the process of sucking out supernatant, and the error of later detection is increased. The device comprises a concentration cylinder and an outer sleeve arranged outside the concentration cylinder, wherein a guide pipe which is communicated up and down is arranged in the concentration cylinder, a stationary liquid injection cavity is formed between the outer wall of the concentration cylinder and the inner wall of the outer sleeve, a piston ring is arranged in the stationary liquid injection cavity, the piston ring is connected with a piston rod, and a plurality of circles of one-way liquid inlet valves are arranged on the side wall of the concentration cylinder from top to bottom at intervals; the guide pipe is sleeved with a lifting sleeve, the lower part of the lifting sleeve is provided with a mounting seat, the side wall of the mounting seat is provided with a ring of annular groove, an annular isolation airbag is mounted in the annular groove, an air vent is arranged in the lifting sleeve, and the isolation airbag is hermetically attached to the inner wall of the concentration barrel after being filled with air so that a cavity above the isolation airbag in the concentration barrel and a cavity below the isolation airbag are hermetically isolated.
Description
Technical Field
The invention relates to a phytoplankton concentration technology, in particular to a phytoplankton concentration device and a method.
Background
At present, investigation and analysis of phytoplankton are essential in ecological environment investigation and research, but because the phytoplankton is small in size and low in density in a water body, a water sample needs to be concentrated during identification and counting. In order to conveniently and quickly concentrate a large number of phytoplankton samples, people invent a plurality of technical schemes, and a common method is to put the samples into a concentration bottle, then add a fixing solution into the concentration bottle, stand the samples for a period of time, remove supernatant liquid in a siphon mode, and leave the concentrated solution. This kind of concentration mode convenient operation, but the stationary liquid mixes unevenly with the sample, makes the sample local rotten phenomenon appear easily, and the supernatant is not good with the concentrate separation, and phytoplankton loses easily in the sample, has increased later stage detection error.
Disclosure of Invention
The invention overcomes the defects that the fixed liquid and the phytoplankton water sample are not uniformly mixed in the phytoplankton concentration process, the phytoplankton is easily taken away in the process of sucking out the supernatant, and the detection error in the later period is increased.
In order to solve the technical problems, the invention adopts the following technical scheme: a phytoplankton concentration device comprises a concentration cylinder and an outer sleeve arranged outside the concentration cylinder, wherein a guide pipe which is communicated up and down is arranged in the concentration cylinder, the upper end of the guide pipe extends out of the concentration cylinder, the lower end of the guide pipe is close to the bottom of the concentration cylinder, a stationary liquid injection cavity is formed between the outer wall of the concentration cylinder and the inner wall of the outer sleeve, a piston ring is arranged in the stationary liquid injection cavity, the piston ring is connected with a piston rod, a plurality of circles of one-way liquid inlet valves are arranged on the side wall of the concentration cylinder at intervals from top to bottom, a discharging hole is formed in the lower end of the concentration cylinder, and a discharging valve is arranged in the discharging hole; the lifting sleeve is sleeved on the guide pipe, the lower portion of the lifting sleeve is provided with a mounting seat, a ring of annular groove is formed in the side wall of the mounting seat, an annular isolation airbag is mounted in the annular groove, a vent hole used for inflating and deflating the isolation airbag is formed in the lifting sleeve, and after the isolation airbag is full of air, the isolation airbag is hermetically attached to the inner wall of the concentration cylinder so that a cavity above the isolation airbag in the concentration cylinder and a cavity below the isolation airbag are hermetically isolated.
When the phytoplankton concentrating device is used, firstly, pouring a quantitative phytoplankton water sample obtained by sampling into a concentrating cylinder; pumping a fixed amount of stationary liquid into the stationary liquid injection cavity through the piston ring; the piston ring is pressed downwards through the piston rod, the fixing liquid in the fixing liquid injection cavity is injected into the concentration cylinder through the one-way liquid inlet valve to fix phytoplankton and prevent the phytoplankton from deteriorating, and the mounting seat at the lower end of the lifting sleeve is arranged above the liquid level in the concentration cylinder; standing the concentration cylinder for 20-25 hours to form a supernatant layer and a phytoplankton concentrated liquid layer in the concentration cylinder; moving the lifting sleeve downwards to enable the mounting seat to slightly enter the upper clear liquid layer until the mounting seat reaches the upper position of the separation position of the upper clear liquid layer and the phytoplankton concentrated liquid layer, inflating the vent holes to enable the isolation air bags to bulge outwards until the isolation air bags are tightly attached to the inner wall of the concentration barrel, and at the moment, completely isolating the upper clear liquid layer from the phytoplankton concentrated liquid layer; placing a receiving cup below the concentration cylinder, opening a discharging valve, and allowing a phytoplankton concentrated liquid layer in the concentration cylinder to fall into the receiving cup under the action of gravity; and after all the phytoplankton concentrated solution falls into the receiving cup, removing the receiving cup, placing a waste liquid cup below the concentrating cylinder, deflating the isolating air bag, and allowing the supernatant to fall into the waste liquid cup through the discharging hole.
From the one-way feed liquor valve of a plurality of circles of interval installation from top to bottom on the concentrated section of thick bamboo lateral wall to pour into the stationary liquid into in the concentrated section of thick bamboo, make the mixing of stationary liquid and phytoplankton water sample more even, make the phytoplankton water sample difficult rotten. And in the separation process of the supernatant and the phytoplankton concentrated solution, the separation is carried out through the separation air bag, so that the supernatant and the phytoplankton concentrated solution are completely separated, then the phytoplankton concentrated solution flows downwards into the material receiving cup, and finally the supernatant flows downwards into the waste liquid cup after the separation air bag is deflated. The phenomenon that the concentrated solution is taken away by the supernatant does not exist in the whole process. The fixing liquid and the phytoplankton water sample are uniformly mixed in the phytoplankton concentration process, the water sample is not easy to deteriorate, the phytoplankton can not be taken away by the supernatant in the separation process of the supernatant and the phytoplankton concentrated liquid, and the later-stage detection accuracy is ensured.
Preferably, the piston ring comprises an outer plug ring and an inner plug ring, the inner plug ring is tightly sleeved in the outer plug ring, the piston rod is connected to the inner plug ring, the outer plug ring is connected with a connecting rod, and the connecting rod is detachably connected with the piston rod; the stationary liquid injection cavity comprises an upper cavity and a lower cavity, the radial width of the upper cavity is larger than that of the lower cavity, a transition surface is arranged between the upper cavity and the lower cavity, the piston ring is matched with the upper cavity, and the inner plug ring can enter the lower cavity.
And the piston ring stops moving after abutting against the transition surface in the mixing stage of the fixing liquid and the phytoplankton water sample. After the phytoplankton concentrated solution completely falls into the receiving cup, the connecting rod is separated from the piston rod, the piston rod is pressed downwards, the inner plug ring is pushed downwards, the fixing liquid in the lower cavity is injected into the position of the phytoplankton concentrated solution layer in the concentrating cylinder through the one-way liquid inlet valve by the inner plug ring, phytoplankton remained on the inner wall of the concentrating cylinder is cleaned, and the cleaned liquid falls downwards into the receiving cup. Greatly reduces the residual phytoplankton on the inner wall of the concentration cylinder and is beneficial to improving the detection accuracy.
Preferably, the upper end of the connecting rod is connected with a locking screw, and the locking screw is in threaded connection with the piston rod. The connecting rod and the piston rod are connected by the locking screw, so that the connection is reliable, and the connection and the disassembly are convenient.
Preferably, the upper ends of the piston rods are connected with the push-pull rings, the piston rings are uniformly connected with a plurality of piston rods, and the upper ends of all the piston rods are connected to the same push-pull ring. The piston rod is pressed and pulled through the pull ring, so that the operation is more convenient.
Preferably, the upper end of the concentration cylinder is uniformly connected with a plurality of supporting rods, the upper part of the guide pipe is connected with a fixing frame, and the upper ends of the supporting rods are fixedly connected with the fixing frame. This arrangement provides sufficient lifting space for the lifting sleeve.
Preferably, the lower part of the concentration cylinder is of a conical structure with a large upper part and a small lower part, the lower end of the concentration cylinder is provided with a blanking pipe, and a blanking hole is arranged in the blanking pipe. This kind of structure setting is convenient for the unloading of concentrate.
Preferably, the lower end of the outer sleeve is communicated with a liquid extracting pipe, and a liquid extracting valve is arranged on the liquid extracting pipe. The arrangement of the liquid extracting pipe and the liquid extracting valve is convenient for the stationary liquid injection cavity to extract the stationary liquid.
Preferably, a fastening screw is connected to an upper portion of the lifting sleeve, and a tip of the fastening screw abuts against an outer wall of the guide tube. The fastening screw is convenient and reliable to lock the lifting sleeve.
A concentration method using a phytoplankton concentration device, comprising the following steps: a. pouring the quantitative phytoplankton water sample obtained by sampling into a concentration cylinder; b. pumping a fixed amount of stationary liquid into the stationary liquid injection cavity through the piston ring; c. the piston ring is pressed downwards through the piston rod, the fixing liquid in the fixing liquid injection cavity is injected into the concentration cylinder through the one-way liquid inlet valve to fix phytoplankton and prevent the phytoplankton from deteriorating, the piston ring stops moving after abutting against the transition surface, and the mounting seat at the lower end of the lifting sleeve is arranged above the liquid level in the concentration cylinder; d. standing the concentration cylinder for 20-25 hours to form a supernatant layer and a phytoplankton concentrated liquid layer in the concentration cylinder; e. moving the lifting sleeve downwards to enable the mounting seat to slightly enter the upper clear liquid layer until the mounting seat reaches the upper position of the separation position of the upper clear liquid layer and the phytoplankton concentrated liquid layer, and inflating the vent holes to enable the isolation air bags to bulge outwards until the isolation air bags are tightly attached to the inner wall of the concentration barrel; f. placing a receiving cup below the concentration cylinder, opening a discharging valve, and allowing a phytoplankton concentrated liquid layer in the concentration cylinder to fall into the receiving cup under the action of gravity; g. after all the phytoplankton concentrated solution falls into the receiving cup, separating the connecting rod from the piston rod, pressing the piston rod downwards, pushing the inner plug ring downwards, injecting the fixing solution in the lower cavity into the position of the phytoplankton concentrated solution layer in the concentration cylinder through the one-way liquid inlet valve by the inner plug ring, cleaning phytoplankton remained on the inner wall of the concentration cylinder, and enabling the cleaned liquid to fall into the receiving cup downwards; h. and (4) removing the material receiving cup, placing the waste liquid cup below the concentration cylinder, deflating the isolation air bag, and allowing the supernatant to fall into the waste liquid cup through the blanking hole.
The fixing liquid and the phytoplankton water sample are uniformly mixed in the phytoplankton concentration process, the water sample is not easy to deteriorate, the phytoplankton can not be taken away by the supernatant in the separation process of the supernatant and the phytoplankton concentrated liquid, and the later-stage detection accuracy is ensured.
Compared with the prior art, the invention has the beneficial effects that: the fixing liquid and the phytoplankton water sample are uniformly mixed in the phytoplankton concentration process, the water sample is not easy to deteriorate, the phytoplankton can not be taken away by the supernatant in the separation process of the supernatant and the phytoplankton concentrated liquid, and the later-stage detection accuracy is ensured.
Drawings
FIG. 1 is a schematic diagram of an embodiment of the present invention;
in the figure: 1. the device comprises a concentration barrel, 2, an outer sleeve, 3, a guide pipe, 4, a fixed liquid injection cavity, 5, a piston rod, 6, a one-way liquid inlet valve, 7, a discharging hole, 8, a discharging valve, 9, a lifting sleeve, 10, a fastening screw, 11, a mounting seat, 12, a ring groove, 13, an isolation air bag, 14, a vent hole, 15, an inflation pipe, 16, an outer plug ring, 17, an inner plug ring, 18, a connecting rod, 19, an upper cavity, 20, a lower cavity, 21, a transition surface, 22, a locking screw, 23, a push-pull ring, 24, a connecting ring, 25, a support rod, 26, a fixing frame, 27, a discharging pipe, 28, a liquid pumping pipe, 29, a support, 30, an upper clear liquid layer, 31 and a phytoplankton concentrated liquid layer.
Detailed Description
The technical scheme of the invention is further described in detail by the following specific embodiments in combination with the attached drawings:
example (b): a phytoplankton concentration device (see attached figure 1) comprises a concentration cylinder 1 and an outer sleeve 2 arranged outside the concentration cylinder, wherein a guide pipe 3 which is communicated up and down is arranged in the concentration cylinder, the upper end of the guide pipe extends out of the concentration cylinder, the lower end of the guide pipe is close to the bottom of the concentration cylinder, a stationary liquid injection cavity 4 is formed between the outer wall of the concentration cylinder and the inner wall of the outer sleeve, a piston ring is arranged in the stationary liquid injection cavity, the piston ring is connected with a piston rod 5, the upper end of the piston rod extends out of the outer sleeve, a plurality of circles of one-way liquid inlet valves 6 are arranged on the side wall of the concentration cylinder at intervals from top to bottom, a plurality of one-way liquid inlet valves are uniformly distributed in each circle, a discharging hole 7 is arranged at the lower end of the concentration cylinder, and a discharging valve 8 is arranged in the discharging hole; the upper portion of the guide pipe is connected with a lifting sleeve 9 in a sleeved mode, the upper portion of the lifting sleeve is connected with a fastening screw 10, the front end of the fastening screw abuts against the outer wall of the guide pipe, the lower portion of the lifting sleeve is provided with a mounting seat 11, a circle of annular groove 12 is formed in the side wall of the mounting seat, an annular isolating air bag 13 is mounted in the annular groove, an air vent 14 used for inflating and deflating the isolating air bag is arranged in the lifting sleeve, the air vent extends to the upper portion of the lifting sleeve, the upper end of the air vent is connected with an air inflation pipe 15, a valve is mounted on the air inflation pipe, and after the isolating air bag is full of air, the isolating air bag is attached to the inner wall of the concentration cylinder in a sealed mode to enable a cavity above the isolating air bag in the concentration cylinder and a cavity below the isolating air bag to be isolated in a sealed mode. The concentrating cylinder and the outer sleeve are transparent.
The piston ring comprises an outer plug ring 16 and an inner plug ring 17, the inner plug ring is tightly sleeved in the outer plug ring, the piston rod is connected to the inner plug ring, the outer plug ring is connected with a connecting rod 18, and the connecting rod is detachably connected with the piston rod; the stationary liquid injection cavity comprises an upper cavity 19 and a lower cavity 20, the radial width of the upper cavity is larger than that of the lower cavity, a transition surface 21 is arranged between the upper cavity and the lower cavity, the transition surface is obliquely arranged from outside to inside downwards, a piston ring is matched with the upper cavity, and an inner plug ring can enter the lower cavity. The lower end surface of the outer plug ring is matched with the transition surface. The upper end of the connecting rod is connected with a locking screw 22 which is in threaded connection with the piston rod. The upper end of the piston rod is connected with a push-pull ring 23, a plurality of piston rods are uniformly distributed and connected on the piston ring, and the upper ends of all the piston rods are connected on the same push-pull ring. The upper ends of the connecting rods are connected with a connecting ring 24, the upper ends of all the connecting rods are fixedly connected with the connecting ring, and the locking screws are connected between the connecting ring and the piston rod.
The upper end of the concentration cylinder is uniformly connected with a plurality of supporting rods 25, the upper part of the guide pipe is connected with a fixed frame 26, and the upper ends of the supporting rods are fixedly connected with the fixed frame. The lower part of the concentration cylinder is of a conical structure with a big top and a small bottom, the lower end of the concentration cylinder is provided with a blanking pipe 27, and a blanking hole is arranged in the blanking pipe. The lower end of the outer sleeve is communicated with a liquid pumping pipe 28, and a liquid pumping valve is arranged on the liquid pumping pipe. The lower end of the outer sleeve is uniformly connected with a plurality of supports 29, and the lower ends of the supports are lower than the blanking pipe, so that blanking of the blanking pipe is facilitated.
A concentration method using a phytoplankton concentration device, comprising the following steps: a. pouring the quantitative phytoplankton water sample obtained by sampling into a concentration cylinder; b. pumping a fixed amount of stationary liquid into a stationary liquid injection cavity through a piston ring, wherein the stationary liquid is Rugowski liquid; c. the piston ring is pressed downwards through the piston rod, the fixing liquid in the fixing liquid injection cavity is injected into the concentration cylinder through the one-way liquid inlet valve to fix phytoplankton and prevent the phytoplankton from deteriorating, the piston ring stops moving after abutting against the transition surface, and the mounting seat at the lower end of the lifting sleeve is arranged above the liquid level in the concentration cylinder; d. standing the concentration cylinder for 20-25 hours to form a supernatant liquid layer 30 and a phytoplankton concentrated liquid layer 31 in the concentration cylinder; e. moving the lifting sleeve downwards to enable the mounting seat to slightly enter the upper clear liquid layer until the mounting seat reaches the upper position of the separation position of the upper clear liquid layer and the phytoplankton concentrated liquid layer, and inflating the vent holes to enable the isolation air bags to bulge outwards until the isolation air bags are tightly attached to the inner wall of the concentration barrel; f. placing a receiving cup below the concentration cylinder, opening a discharging valve, and allowing a phytoplankton concentrated liquid layer in the concentration cylinder to fall into the receiving cup under the action of gravity; g. after all the phytoplankton concentrated solution falls into the receiving cup, separating the connecting rod from the piston rod, pressing the piston rod downwards, pushing the inner plug ring downwards, injecting the fixing solution in the lower cavity into the position of the phytoplankton concentrated solution layer in the concentration cylinder through the one-way liquid inlet valve by the inner plug ring, cleaning phytoplankton remained on the inner wall of the concentration cylinder, and enabling the cleaned liquid to fall into the receiving cup downwards; h. and (4) removing the material receiving cup, placing the waste liquid cup below the concentration cylinder, deflating the isolation air bag, and allowing the supernatant to fall into the waste liquid cup through the blanking hole.
The above-described embodiments are merely preferred embodiments of the present invention, which is not intended to be limiting in any way, and other variations and modifications are possible without departing from the scope of the invention as set forth in the appended claims.
Claims (9)
1. A phytoplankton concentration device is characterized by comprising a concentration cylinder and an outer sleeve arranged outside the concentration cylinder, wherein a guide pipe which is communicated up and down is arranged in the concentration cylinder, the upper end of the guide pipe extends out of the concentration cylinder, the lower end of the guide pipe is close to the bottom of the concentration cylinder, a stationary liquid injection cavity is formed between the outer wall of the concentration cylinder and the inner wall of the outer sleeve, a piston ring is arranged in the stationary liquid injection cavity, the piston ring is connected with a piston rod, a plurality of circles of one-way liquid inlet valves are arranged on the side wall of the concentration cylinder at intervals from top to bottom, a discharging hole is formed in the lower end of the concentration cylinder, and a discharging valve is arranged in the discharging hole; the lifting sleeve is sleeved on the guide pipe, the lower portion of the lifting sleeve is provided with a mounting seat, a ring of annular groove is formed in the side wall of the mounting seat, an annular isolation airbag is mounted in the annular groove, a vent hole used for inflating and deflating the isolation airbag is formed in the lifting sleeve, and after the isolation airbag is full of air, the isolation airbag is hermetically attached to the inner wall of the concentration cylinder so that a cavity above the isolation airbag in the concentration cylinder and a cavity below the isolation airbag are hermetically isolated.
2. The phytoplankton concentrating device according to claim 1, wherein the piston ring comprises an outer plug ring and an inner plug ring, the inner plug ring is tightly sleeved in the outer plug ring, the piston rod is connected to the inner plug ring, the outer plug ring is connected with a connecting rod, and the connecting rod is detachably connected with the piston rod; the stationary liquid injection cavity comprises an upper cavity and a lower cavity, the radial width of the upper cavity is larger than that of the lower cavity, a transition surface is arranged between the upper cavity and the lower cavity, the piston ring is matched with the upper cavity, and the inner plug ring can enter the lower cavity.
3. The phytoplankton concentrating device according to claim 2, wherein the upper end of the connecting rod is connected with a locking screw, and the locking screw is in threaded connection with the piston rod.
4. The phytoplankton concentrating device according to claim 1, wherein the upper end of the piston rod is connected with a push-pull ring, a plurality of piston rods are uniformly connected to the piston ring, and the upper ends of all the piston rods are connected to the same push-pull ring.
5. The phytoplankton concentrating device according to claim 1, wherein a plurality of support rods are uniformly connected to the upper end of the concentrating cylinder, the upper part of the guide pipe is connected with a fixing frame, and the upper ends of the support rods are fixedly connected with the fixing frame.
6. The phytoplankton concentrating device according to any one of claims 1 to 5, wherein the lower part of the concentrating cylinder is in a conical structure with a large upper part and a small lower part, the lower end of the concentrating cylinder is provided with a blanking pipe, and a blanking hole is arranged in the blanking pipe.
7. The phytoplankton concentrating device according to any one of claims 1 to 5, wherein the lower end of the outer sleeve is communicated with a liquid extracting pipe, and a liquid extracting valve is arranged on the liquid extracting pipe.
8. The phytoplankton concentrating apparatus according to any one of claims 1 to 5, wherein a fastening screw is connected to the upper part of the lifting sleeve, and the front end of the fastening screw abuts against the outer wall of the guide pipe.
9. A concentration method using the phytoplankton concentration apparatus according to claim 2, comprising the steps of: a. pouring the quantitative phytoplankton water sample obtained by sampling into a concentration cylinder; b. pumping a fixed amount of stationary liquid into the stationary liquid injection cavity through the piston ring; c. the piston ring is pressed downwards through the piston rod, the fixing liquid in the fixing liquid injection cavity is injected into the concentration cylinder through the one-way liquid inlet valve to fix phytoplankton and prevent the phytoplankton from deteriorating, the piston ring stops moving after abutting against the transition surface, and the mounting seat at the lower end of the lifting sleeve is arranged above the liquid level in the concentration cylinder; d. standing the concentration cylinder for 20-25 hours to form a supernatant layer and a phytoplankton concentrated liquid layer in the concentration cylinder; e. moving the lifting sleeve downwards to enable the mounting seat to slightly enter the upper clear liquid layer until the mounting seat reaches the upper position of the separation position of the upper clear liquid layer and the phytoplankton concentrated liquid layer, and inflating the vent holes to enable the isolation air bags to bulge outwards until the isolation air bags are tightly attached to the inner wall of the concentration barrel; f. placing a receiving cup below the concentration cylinder, opening a discharging valve, and allowing a phytoplankton concentrated liquid layer in the concentration cylinder to fall into the receiving cup under the action of gravity; g. after all the phytoplankton concentrated solution falls into the receiving cup, separating the connecting rod from the piston rod, pressing the piston rod downwards, pushing the inner plug ring downwards, injecting the fixing solution in the lower cavity into the position of the phytoplankton concentrated solution layer in the concentration cylinder through the one-way liquid inlet valve by the inner plug ring, cleaning phytoplankton remained on the inner wall of the concentration cylinder, and enabling the cleaned liquid to fall into the receiving cup downwards; h. and (4) removing the material receiving cup, placing the waste liquid cup below the concentration cylinder, deflating the isolation air bag, and allowing the supernatant to fall into the waste liquid cup through the blanking hole.
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Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
TW219349B (en) * | 1992-04-11 | 1994-01-21 | Kuwashima Hiroshi | |
CN101810947A (en) * | 2010-04-14 | 2010-08-25 | 湖南省水产科学研究所 | phytoplankton separator |
CN103439175A (en) * | 2013-08-30 | 2013-12-11 | 中国科学院水生生物研究所 | Portable concentration device for quantitative samples of planktons |
CN204128876U (en) * | 2014-11-13 | 2015-01-28 | 中国水产科学研究院长江水产研究所 | A kind of assembling phytoplankton subsiding device top cover |
CN105158048A (en) * | 2015-08-18 | 2015-12-16 | 浙江省海洋水产研究所 | Suction type solid-liquid separator |
CN106110716A (en) * | 2016-07-13 | 2016-11-16 | 浙江省海洋水产研究所 | A kind of microzooplankton settling separation device and separation method |
CN107996530A (en) * | 2017-12-25 | 2018-05-08 | 百奥森(江苏)食品安全科技有限公司 | A kind of plankton sampling device |
-
2019
- 2019-09-17 CN CN201910876421.3A patent/CN110736656B/en active Active
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
TW219349B (en) * | 1992-04-11 | 1994-01-21 | Kuwashima Hiroshi | |
CN101810947A (en) * | 2010-04-14 | 2010-08-25 | 湖南省水产科学研究所 | phytoplankton separator |
CN103439175A (en) * | 2013-08-30 | 2013-12-11 | 中国科学院水生生物研究所 | Portable concentration device for quantitative samples of planktons |
CN204128876U (en) * | 2014-11-13 | 2015-01-28 | 中国水产科学研究院长江水产研究所 | A kind of assembling phytoplankton subsiding device top cover |
CN105158048A (en) * | 2015-08-18 | 2015-12-16 | 浙江省海洋水产研究所 | Suction type solid-liquid separator |
CN106110716A (en) * | 2016-07-13 | 2016-11-16 | 浙江省海洋水产研究所 | A kind of microzooplankton settling separation device and separation method |
CN107996530A (en) * | 2017-12-25 | 2018-05-08 | 百奥森(江苏)食品安全科技有限公司 | A kind of plankton sampling device |
Non-Patent Citations (2)
Title |
---|
"Phytoplankton processes during a mesoscale iron enrichment in the NE subarctic Pacific: Part I—Biomass and assemblage";AdrianMarchetti 等;《Deep Sea Research Part II: Topical Studies in Oceanography》;20061231;第53卷(第20-22期);第2095-2113页 * |
"湖泊蓝藻水华发生机理研究进展";马健荣 等;《生态学报》;20131231;第33卷(第10期);第3020-3030页 * |
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