CN110724683A - Carbon-based microbial composite bacteria powder, microbial inoculum and preparation method and application thereof - Google Patents
Carbon-based microbial composite bacteria powder, microbial inoculum and preparation method and application thereof Download PDFInfo
- Publication number
- CN110724683A CN110724683A CN201911148942.3A CN201911148942A CN110724683A CN 110724683 A CN110724683 A CN 110724683A CN 201911148942 A CN201911148942 A CN 201911148942A CN 110724683 A CN110724683 A CN 110724683A
- Authority
- CN
- China
- Prior art keywords
- carbon
- composite bacterial
- bacillus
- microbial composite
- bacterial powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Inorganic Chemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides carbon-based microbial composite bacterial powder, a microbial inoculum and a preparation method and application thereof, belonging to the technical field of microbes, wherein microbes are adsorbed on a solid carrier to obtain the carbon-based microbial composite microbial inoculum; the solid support comprises biochar and zeolite; the microorganisms include bacillus and streptococcus faecalis; the mass ratio of the solid carrier to the microorganism is 40-60: 1. According to the invention, the high porosity and the high specific surface area of the biochar are utilized, and the cheap high-adsorbability material zeolite is matched, so that the treatment performance of a single microbial inoculum on the livestock and poultry breeding sewage is improved, the pH stability of a water body is maintained, the residues of nitrogen, phosphorus, organic pollutants and harmful heavy metals in the sewage are effectively reduced, and the pollution degree of the livestock and poultry breeding sewage on the surrounding water environment is reduced.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to carbon-based microorganism composite bacteria powder, a microbial inoculum, and a preparation method and application thereof.
Background
With the rapid development of the large-scale, intensive and modernized livestock and poultry breeding industry in China, the discharge amount of livestock and poultry breeding sewage is increased sharply, and the problem of breeding pollution is more and more prominent. According to incomplete statistics, in 2011, the output of livestock and poultry feces and urine in China is about 27 hundred million tons, which is about 2 times of the total discharge amount of industrial solid wastes in the same year; the livestock and poultry manure contains a large amount of organic pollutants, the COD is 1.2 hundred million tons only, which far exceeds the total COD in industrial and domestic wastes, and the COD, the industrial sewage and the domestic sewage are one of three major sources of water environment pollution. The high-concentration livestock and poultry breeding sewage is discharged into rivers and lakes, on one hand, algae and plankton in the water can be propagated in a large quantity, the water is seriously eutrophicated, the water is anoxic, and aquatic animals such as fishes and the like die in a large quantity; on the other hand, some harmful substances may permeate into the groundwater, causing deterioration of the groundwater or drinking water quality, and directly endangering human health.
The livestock and poultry breeding sewage is complex in composition and covers a large amount of organic and inorganic pollutants, and particularly nitrogen, phosphorus, heavy metals and organic matters are representative pollutants which are difficult to treat. At present, the livestock and poultry breeding sewage treatment method mainly adopts a biological method, and converts organic pollutants in the wastewater into stable and harmless substances through the metabolic action of effective microbiota. In order to improve the sewage treatment effect, two or more than two treatment methods are generally adopted in the actual treatment process, namely, a physical adsorption method, a chemical precipitation method, an advanced oxidation method, a biological method and an artificial wetland method are comprehensively utilized to treat sewage. Although the methods can remove nitrogen, phosphorus, organic matters and heavy metals in part of wastewater, the methods have the defects of large investment, high running cost, complex treatment process and the like, and the effects are not ideal. Therefore, the development of a treatment means with low cost, simple process, stable effect, simple and convenient operation and high performance is sought, and becomes an important task for solving the problem of livestock and poultry breeding wastewater pollution.
Disclosure of Invention
In view of the above, the present invention aims to provide a carbon-based microbial composite bacterial powder, a microbial inoculum, and a preparation method and an application thereof.
In order to achieve the above purpose, the invention provides the following technical scheme:
the invention provides carbon-based microorganism composite bacterial powder, which is prepared by adsorbing microorganisms on a solid carrier;
the solid support comprises biochar and zeolite;
the microorganisms include bacillus and streptococcus faecalis;
the mass ratio of the solid carrier to the microorganism is 40-60: 1.
Preferably, the mass ratio of the biochar to the zeolite is 20-30: 20-30.
Preferably, the mass ratio of the bacillus to the streptococcus faecalis is 4-6: 2.
Preferably, the bacillus includes bacillus subtilis, bacillus licheniformis, bacillus mucilaginosus and bacillus pumilus.
Preferably, the mass ratio of the bacillus subtilis to the bacillus licheniformis to the bacillus mucilaginosus to the bacillus pumilus is 1-3: 1-3.
The invention also provides application of the carbon-based microbial composite bacterial powder in the technical scheme in treatment of livestock and poultry breeding sewage.
The invention also provides a carbon-based microbial composite microbial inoculum, which comprises the carbon-based microbial composite microbial powder, a trace element material and a drying agent in the technical scheme;
the mass ratio of the carbon-based microorganism composite bacterial powder to the trace element material to the drying agent is 70-80: 2-15: 8-15.
Preferably, the trace element materials comprise zinc sulfate, magnesium sulfate and borax;
the desiccant comprises one or more of bentonite, silica gel, white carbon black and attapulgite.
The invention also provides application of the carbon-based microbial composite inoculant in the technical scheme in treatment of livestock and poultry breeding sewage.
The invention also provides a preparation method of the carbon-based microbial composite inoculant in the technical scheme, which comprises the following steps:
1) carrying out liquid state fermentation on microorganisms to obtain fermentation liquor, and adsorbing, drying and crushing the fermentation liquor by using a solid carrier to obtain carbon-based microorganism composite bacterial powder;
2) mixing the carbon-based microbial composite bacterial powder obtained in the step 1) with trace element materials and drying agents to obtain the carbon-based microbial composite bacterial agent.
The invention provides carbon-based microorganism composite bacteria powder, a microbial inoculum and a preparation method and application thereof, wherein microorganisms are adsorbed on a solid carrier to obtain the carbon-based microorganism composite bacteria powder; the solid support comprises biochar and zeolite; the microorganisms include bacillus and streptococcus faecalis; the mass ratio of the solid carrier to the microorganism is 40-60: 1.
According to the invention, the high porosity and the high specific surface area of the biochar are utilized, and the cheap high-adsorbability material zeolite is matched, so that the treatment performance of a single microbial inoculum on the livestock and poultry breeding sewage is improved, the pH stability of a water body is maintained, the residues of nitrogen, phosphorus, organic pollutants and harmful heavy metals in the sewage are effectively reduced, and the pollution degree of the livestock and poultry breeding sewage on the surrounding water environment is reduced.
The results of the embodiment of the invention show that after the carbon-based microbial composite inoculant is used for water bodies with pH of 8.34, nitrate nitrogen of 46.35mg/L, nitrite nitrogen of 76.50mg/L, total phosphorus of 43.97mg/L, active phosphate of 16.71mg/L, organic pollutants of 10mg/L and harmful heavy metals of 10mg/L, the pH of the water bodies is stable, the nitrate nitrogen is reduced to 36.69mg/L, the nitrite nitrogen is reduced to 56.67mg/L, the total phosphorus is reduced to 20.12mg/L, the active phosphate is reduced to 8.62mg/L, the organic pollutants are reduced to 2.97-7.72 mg/L, the heavy metals are reduced to 0-1.62 mg/L, the total removal rate can reach 20.85-100%, and the treatment performance is superior to that of a single inoculant. In addition, the method takes the waste materials generated by processing the bamboo products as the precursor to prepare the biochar carrier, improves the treatment efficiency, reduces the cost, is easy to obtain, and is favorable for realizing the recycling of wastes.
Drawings
Figure 1 shows the effect of different adsorbents on pH of pig manure water, which represents the very significant level (P <0.01) and significant level (P <0.05) of the difference between different treatments, respectively;
FIG. 2 shows the application of different adsorbents to nitrate Nitrogen (NO) in pig manure3-N) and nitrous Nitrogen (NO)2-N) concentration, and represents the achievement of a very significant level of difference (P) between the different treatments, respectively<0.01) and significant levels (P)<0.05)；
Figure 3 is a graph of the effect of different adsorbents on Total Phosphorus (TP) and active phosphate (PO4) concentrations in pig manure, representing very significant levels (P <0.01) and significant levels (P <0.05) of the difference between different treatments, respectively;
figure 4 is the effect of different adsorbents on the concentration of organic contaminants in simulated water samples, which represent the very significant level (P <0.01) and significant level (P <0.05) of the difference between different treatments, respectively;
figure 5 shows the effect of different adsorbents on the concentration of heavy metals in simulated water samples (//was not detected), and represents the very significant level (P <0.01) and significant level (P <0.05) of the difference between the different treatments, respectively.
Detailed Description
The invention provides carbon-based microorganism composite bacterial powder, which is prepared by adsorbing microorganisms on a solid carrier; the solid support comprises biochar and zeolite; the microorganisms include bacillus and streptococcus faecalis; the mass ratio of the solid carrier to the microorganism is 40-60: 1.
In the invention, the solid carrier comprises biochar and zeolite, and the mass ratio of the biochar to the zeolite is preferably 20-30: 20-30, and more preferably 25: 25. The source of the biochar is not particularly limited, and the biochar can be prepared by adopting a conventional commercial product or a conventional method. In a specific embodiment of the present invention, the method for preparing biochar preferably comprises: thermally cracking the waste materials left after the processing of the bamboo products for 1-5 hours at 450-550 ℃ under an oxygen-free condition, and sieving the crushed waste materials with a sieve of 80-100 meshes, wherein the organisms under the sieve are biochar. The source of the zeolite is not particularly limited in the present invention, and the zeolite can be prepared by a commercially available product or by a conventional preparation method, and in a specific embodiment of the present invention, the preparation method of the zeolite preferably comprises: and (3) absorbing moisture of the zeolite, drying, crushing, and sieving with a 180-200-mesh sieve, wherein the sieved substances are the zeolite. In the present invention, the biochar and zeolite serve as carriers for adsorbing microorganisms.
In the invention, the microorganisms comprise bacillus and streptococcus faecalis, and the mass ratio of the bacillus to the streptococcus faecalis is preferably 4-6: 2. In the invention, the bacillus preferably comprises bacillus subtilis, bacillus licheniformis, bacillus mucilaginosus and bacillus pumilus, and the mass ratio of the bacillus subtilis to the bacillus licheniformis to the bacillus mucilaginosus to the bacillus pumilus is preferably 1-3: 1-3. The source of the microorganism is not particularly limited, the microorganism is a conventional strain, in the specific embodiment of the invention, the preservation number of the bacillus subtilis is preferably CGMCC1.15792, the preservation number of the bacillus licheniformis is preferably CGMCC1.10314, the preservation number of the bacillus mucilaginosus is preferably CGMCC1.2326, the preservation number of the bacillus pumilus is preferably CGMCC1.10291, and the preservation number of the streptococcus faecalis is preferably CGMCC 1.15321. In the invention, the bacillus forms dominant flora in water environment, strongly decomposes pollutants of carbon series, nitrogen series, phosphorus series and sulfur series, decomposes complex polysaccharide, protein and water-soluble organic matters, and has the function of purifying water quality; the streptococcus faecalis is used for rapidly degrading organic matters in a water body, including excrement of cultured animals, residual feed, planktonic algae corpses and the like, so that inorganic salts such as nitrates, phosphates, sulfates and the like are generated, the chemical oxygen demand and the biochemical oxygen demand in the water are effectively reduced, the concentrations of ammonia nitrogen, nitrite nitrogen and sulfides in the water body are reduced, and the water quality is effectively improved.
In the present invention, the preparation method of the carbon-based microbial composite bacterial powder preferably comprises: carrying out liquid state fermentation on the microorganism to obtain fermentation liquor, and carrying out adsorption, drying and crushing on the fermentation liquor by using a solid carrier to obtain the carbon-based microorganism composite bacterial powder. The method for carrying out liquid fermentation on the microorganisms is not particularly limited, and the microorganisms can be obtained by adopting a conventional liquid fermentation method, wherein the microorganisms are respectively fermented, and the obtained bacterial liquids are mixed to obtain fermentation liquor. In the invention, the content of viable bacteria in the bacterial liquid is preferably 200 hundred million cfu/ml. In the present invention, the drying temperature is preferably 30 to 40 ℃. In the invention, the crushed materials are preferably sieved by a 40-mesh sieve, and the undersize products are carbon-based microorganism composite bacteria powder.
The invention also provides application of the carbon-based microbial composite bacterial powder in the technical scheme in treatment of livestock and poultry breeding sewage. The application of the invention is not specially limited, and the carbon-based microorganism composite bacterial powder is mixed with livestock and poultry breeding sewage.
The invention also provides a carbon-based microbial composite microbial inoculum, which comprises the carbon-based microbial composite microbial powder, a trace element material and a drying agent in the technical scheme; the mass ratio of the carbon-based microorganism composite bacterial powder to the trace element material to the drying agent is 70-80: 2-15: 8-15.
In the invention, the trace element material preferably comprises zinc sulfate, magnesium sulfate and borax, the mass ratio of the zinc sulfate to the magnesium sulfate to the borax is preferably 1:2:1, and the trace element material provides inorganic salt required by growth for the growth of microorganisms; the desiccant comprises one or more of bentonite, silica gel, white carbon black and attapulgite. The source of the reagent is not particularly limited in the present invention, and a conventional commercially available product may be used.
The invention also provides application of the carbon-based microbial composite inoculant in the technical scheme in treatment of livestock and poultry breeding sewage. The application of the invention is not specially limited, and the carbon-based microbial composite inoculant is mixed with livestock and poultry breeding sewage.
The invention also provides a preparation method of the carbon-based microbial composite inoculant in the technical scheme, which comprises the following steps:
1) carrying out liquid state fermentation on microorganisms to obtain fermentation liquor, and adsorbing, drying and crushing the fermentation liquor by using a solid carrier to obtain carbon-based microorganism composite bacterial powder;
2) mixing the carbon-based microbial composite bacterial powder obtained in the step 1) with trace element materials and drying agents to obtain the carbon-based microbial composite bacterial agent.
In the invention, the preparation method of the carbon-based microbial composite bacterial powder comprises the following steps: carrying out liquid state fermentation on the microorganism to obtain fermentation liquor, and carrying out adsorption, drying and crushing on the fermentation liquor by using a solid carrier to obtain the carbon-based microorganism composite bacterial powder. The method for carrying out liquid fermentation on the microorganisms is not particularly limited, and the microorganisms can be obtained by adopting a conventional liquid fermentation method, wherein the microorganisms are respectively fermented, and the obtained bacterial liquids are mixed to obtain fermentation liquor. In the invention, the content of viable bacteria in the bacterial liquid is preferably 200 hundred million cfu/ml. In the present invention, the drying temperature is preferably 30 to 40 ℃. In the invention, the crushed materials are preferably sieved by a 40-mesh sieve, and the undersize products are carbon-based microorganism composite bacteria powder.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
Preparing carbon-based microbial composite bacterial powder:
1) sequentially carrying out seed liquid culture and liquid fermentation culture on various microorganisms for sewage treatment to obtain a composite microorganism fermentation liquor;
2) mixing and adsorbing the composite microbial fermentation liquid, the biochar and the zeolite carrier according to the mass ratio of 1:25:25 to prepare carbon-based carrier microbial composite bacterial liquid;
3) oven drying at 30 deg.C, pulverizing, sieving with 40 mesh sieve to obtain carbon-based microorganism composite bacteria powder with water content below 15%.
The microorganism is bacillus and streptococcus faecalis, the mass ratio is 3:1, the bacillus is bacillus subtilis (CGMCC1.15792), bacillus licheniformis (CGMCC1.10314), bacillus mucilaginosus (CGMCC1.2326) and bacillus pumilus (CGMCC1.10291), and the preservation number of the streptococcus faecalis is CGMCC 1.15321.
The microbial seed liquid culture for sewage treatment comprises the following steps:
respectively inoculating and activating the compound microorganism strains for sewage treatment in an activation culture medium for 24 hours at the temperature of 20 ℃ under a closed condition, and continuously stirring at the rotating speed of 180rpm during the period to respectively obtain single strain seed solutions; the activation medium is prepared from 5g of beef extract, 5g of peptone, 5g of sodium chloride and 1000ml of distilled water, and the pH value is 7.2. Wherein the viable count of the seed liquid of a single strain is 200 hundred million cfu/ml.
The liquid fermentation broth culture of the microorganism for sewage treatment comprises the following steps:
respectively inoculating the single strain seed liquid into a liquid fermentation culture medium according to the inoculation amount of 10% of the volume ratio, fermenting and propagating for 24 hours at the temperature of 20 ℃, and meanwhile, continuously stirring at the rotating speed of 180 rpm; respectively obtaining single strain fermentation liquor, and mixing the single strain fermentation liquor with the mass ratio of 1:1:1:1:1 to obtain the composite microorganism fermentation liquor.
The liquid fermentation medium is prepared from 10kg of starch, 10kg of bean cake powder, 1kg of peptone, 1kg of ammonium sulfate, 0.5kg of monopotassium phosphate, 0.1kg of yeast powder and 5kg of calcium carbonate, the pH is adjusted to 7.5, and the volume is fixed to 1t by water; the aeration rate of aerobic fermentation is 0.6V/V.min.
The biochar and the zeolite are prepared by the following method:
crushing and cutting waste materials generated in the processing process of bamboo products, drying, thermally cracking for 5 hours at the high temperature of 450 ℃ under the anoxic condition, cooling, crushing, and sieving with a 80-mesh sieve to form bamboo charcoal powder.
Absorbing moisture and drying zeolite, cooling, pulverizing, sieving with 180 mesh sieve to obtain zeolite powder, mixing with bamboo charcoal powder at a ratio of 1:1, and making into carrier for microorganism.
Example 2
Preparing carbon-based microbial composite bacterial powder:
1) sequentially carrying out seed liquid culture and liquid fermentation culture on various microorganisms for sewage treatment to obtain a composite microorganism fermentation liquor;
2) mixing and adsorbing the composite microbial fermentation liquor, charcoal and zeolite carriers according to the mass ratio of 1:20:20 to prepare carbon-based carrier microbial composite bacterial liquid;
3) oven drying at 30 deg.C, pulverizing, sieving with 40 mesh sieve to obtain carbon-based microorganism composite bacteria powder with water content below 15%.
The other conditions were the same as in example 1.
Example 3
Preparing carbon-based microbial composite bacterial powder:
1) sequentially carrying out seed liquid culture and liquid fermentation culture on various microorganisms for sewage treatment to obtain a composite microorganism fermentation liquor;
2) mixing and adsorbing the composite microbial fermentation liquor, the biochar and the zeolite carrier according to the mass ratio of 1:30:30 to prepare carbon-based carrier microbial composite bacterial liquid;
3) oven drying at 30 deg.C, pulverizing, sieving with 40 mesh sieve to obtain carbon-based microorganism composite bacteria powder with water content below 15%.
The other conditions were the same as in example 1.
Example 4
Carbon-based microbial composite inoculant: 70 parts of the carbon-based microbial composite bacterial powder obtained in example 1, 2 parts of trace element material and 8 parts of drying agent are mixed to obtain the carbon-based microbial composite bacterial agent.
Wherein the trace element materials comprise zinc sulfate, magnesium sulfate and borax in a mass ratio of 1:2: 1; the drying agent is bentonite.
Example 5
Carbon-based microbial composite inoculant: 80 parts of the carbon-based microbial composite bacterial powder obtained in example 1, 15 parts of trace element material and 15 parts of drying agent are mixed to obtain the carbon-based microbial composite bacterial agent.
Wherein the trace element materials comprise zinc sulfate, magnesium sulfate and borax in a mass ratio of 1:2: 1; the drying agent is bentonite.
Example 6
Carbon-based microbial composite inoculant: 75 parts of the carbon-based microbial composite bacterial powder obtained in example 1, 10 parts of trace element material and 10 parts of drying agent are mixed to obtain the carbon-based microbial composite bacterial agent.
Wherein the trace element materials comprise zinc sulfate, magnesium sulfate and borax in a mass ratio of 1:2: 1; the drying agent is bentonite.
Example 7
Collecting pig manure from a pig farm of a comprehensive test station of Shanghai agricultural academy of sciences, weighing 0.1g of the carbon-based microbial composite inoculum prepared in example 4 into a 250mL conical flask, adding 100mL of pig manure water, placing the pig manure water without an adsorbent as a reference, oscillating the pig manure water in a constant-temperature oscillator at 25 ℃ at a rotating speed of 100r/min, filtering the mixture after 24 hours to obtain a supernatant, and measuring the concentrations of pH, nitrate nitrogen, nitrite nitrogen, total phosphorus and active phosphate, wherein the results are shown in figures 1-3 and table 1.
TABLE 1 treatment effect of carbon-based composite microbial inoculum on swine waste water
| Treatment of | pH | Nitrate nitrogen (mg/kg) | Nitrous nitrogen (mg/kg) | Total phosphorus (mg/kg) | Active phosphate (mg/kg) |
| Control | 8.34 | 46.35 | 76.5 | 43.97 | 16.71 |
| Example 4 | 8.34 | 36.69 | 56.67 | 20.12 | 8.62 |
Remarking: the parenthesized data in the table is the removal rate of nitrogen and phosphorus pollutants
The measured pH was 8.34, the removal rate of nitrate nitrogen was 20.85%, the removal rate of nitrite nitrogen was 25.93%, the removal rate of total phosphorus was 54.23%, and the removal rate of active phosphate was 48.39%.
Comparative example 1
Preparing a compound microbial agent:
1) sequentially carrying out seed liquid culture and liquid fermentation culture on various microorganisms for sewage treatment to obtain a composite microorganism fermentation liquor;
2) mixing and adsorbing the composite microbial fermentation liquid and diatomite according to the mass ratio of 1: 4-6 to obtain a carbon-based carrier microbial composite bacterial liquid;
3) oven drying at 30 deg.C, pulverizing, sieving with 40 mesh sieve to obtain carbon-based microorganism composite bacteria powder with water content below 15%.
4) And mixing 70 parts of carbon-based microbial composite bacterial powder, 2 parts of trace element material and 8 parts of drying agent to obtain the composite microbial agent.
The microbial species, seed liquid fermentation and liquid fermentation were the same as in example 1, and the types of the trace element materials and the drying agents were the same as in example 1.
Comparative example 2
Collecting pig manure from pig farms in comprehensive test stations of villages and villages of Shanghai agricultural sciences, weighing 0.1g of the compound microbial inoculum prepared in comparative example 1 into a 250mL conical flask, adding 100mL of pig manure, placing the conical flask in a constant-temperature oscillator at 25 ℃ for oscillation at a rotating speed of 100r/min by taking the pig manure without adding an adsorbent as a reference, filtering to obtain a supernatant after 24 hours, and measuring the concentrations of pH, nitrate nitrogen, nitrite nitrogen, total phosphorus and active phosphate, wherein the results are shown in figures 1-3 and table 2.
TABLE 2 treatment effect of complex microbial inoculant on swine waste water
| Treatment of | pH | Nitrate nitrogen (mg/kg) | Nitrous nitrogen (mg/kg) | Total phosphorus (mg/kg) | Active phosphate (mg/kg) |
| Control | 8.34 | 46.35 | 76.5 | 43.97 | 16.71 |
| Comparative example 1 | 8.69 | 45.03 | 88.67 | 26.67 | 10.52 |
The measured pH was 8.69, the removal rate of nitrate nitrogen was 2.84%, the removal rate of nitrite nitrogen was-15.9% (no reduction in nitrite nitrogen), the removal rate of total phosphorus was 32.48%, and the removal rate of active phosphate was 37.03%.
Example 8
The test adopts a simulated water sample, and adopts carbamazepine, ibuprofen and bisphenol A to prepare cadmium chloride (CdCl) with the organic pollutant mass concentration of 10mg/L2·2.5H2O), chromium chloride (CrCl)3·6H2O) and copper sulfate (CuSO)4·5H2O) preparing a simulated water sample with the mass concentration of heavy metals of cadmium (Cd), chromium (Cr) and copper (Cu) being 10 mg/L. 0.1g of the carbon-based microbial compound inoculum prepared in example 4 is weighed in a 250mL conical flask, 100mL of simulated water sample is added, and the simulated water sample without the adsorbent is used as a control and is placed inOscillating at the rotation speed of 100r/min in a constant temperature oscillator at the temperature of 25 ℃, filtering and taking supernatant after 24 hours, and respectively measuring the concentrations of organic pollutants (carbamazepine, ibuprofen and bisphenol A) and heavy metals (Cd, Cr and Cu) by adopting a high performance liquid chromatograph and a flame atomic absorption spectrometer, wherein the results are shown in a graph of 4-5 and a table 3.
TABLE 3 treatment effect of carbon-based composite microbial inoculum on simulated water sample
Remarking: the parenthesized data in the table is the removal rate of organic pollutants/heavy metals
The removal rate of carbamazepine was 60.85%, the removal rate of ibuprofen was 25.11%, the removal rate of bisphenol a was 66.59%, the removal rate of Cd was 81.63%, the removal rate of Cr was 96.50%, and the removal rate of Cu was 100%.
Comparative example 3
The test adopts a simulated water sample, and adopts carbamazepine, ibuprofen and bisphenol A to prepare cadmium chloride (CdCl) with the organic pollutant mass concentration of 10mg/L2·2.5H2O), chromium chloride (CrCl)3·6H2O) and copper sulfate (CuSO)4·5H2O) preparing a simulated water sample with the mass concentration of heavy metals of cadmium (Cd), chromium (Cr) and copper (Cu) being 10 mg/L. Weighing 0.1g of the compound microbial agent prepared in the comparative example 1 into a 250mL conical flask, adding 100mL of a simulated water sample, taking the simulated water sample without the adsorbent as a reference, placing the sample in a constant temperature oscillator at 25 ℃ and oscillating at the rotating speed of 100r/min, after 24h, filtering to obtain a supernatant, and respectively measuring the concentrations of organic pollutants (carbamazepine, ibuprofen and bisphenol A) and heavy metals (Cd, Cr and Cu) by using a high performance liquid chromatograph and a flame atomic absorption spectrometer, wherein the results are shown in FIGS. 4-5 and Table 4.
TABLE 4 treatment effect of complex microbial inoculant on simulated water sample
Remarking: the parenthesized data in the table is the removal rate of organic contaminants/heavy metals.
The removal rate of carbamazepine was 1.54%, the removal rate of ibuprofen was 34.67%, the removal rate of bisphenol a was 3.08%, the removal rate of Cd was 34.66%, the removal rate of Cr was 86.36%, and the removal rate of Cu was 98.94%.
According to the embodiment and the comparative example, after the carbon-based microbial composite inoculant is used for a water body with the pH value of 8.34, the nitrate nitrogen content of 46.35mg/L, the nitrite nitrogen content of 76.50mg/L, the total phosphorus content of 43.97mg/L, the active phosphate content of 16.71mg/L, the organic pollutants of 10mg/L and the harmful heavy metals of 10mg/L, the pH value of the water body is stable, the nitrate nitrogen content is reduced to 36.69mg/L, the nitrite nitrogen content is reduced to 56.67mg/L, the total phosphorus content is reduced to 20.12mg/L, the active phosphate content is reduced to 8.62mg/L, the organic pollutants are reduced to 2.97-7.72 mg/L, the heavy metals are reduced to 0-1.62 mg/L, the total removal rate can reach 20.85-100%, and the treatment performance is superior to that of a single inoculant.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (10)
1. The carbon-based microorganism composite bacterial powder is characterized in that microorganisms are adsorbed on a solid carrier to obtain the carbon-based microorganism composite bacterial powder;
the solid support comprises biochar and zeolite;
the microorganisms include bacillus and streptococcus faecalis;
the mass ratio of the solid carrier to the microorganism is 40-60: 1.
2. The carbon-based microbial composite bacterial powder according to claim 1, wherein the mass ratio of the biochar to the zeolite is 20-30: 20-30.
3. The carbon-based microbial composite bacterial powder according to claim 1, wherein the mass ratio of the bacillus to the streptococcus faecalis is 4-6: 2.
4. The carbon-based microbial composite bacterial powder according to claim 1 or 3, wherein the bacillus comprises bacillus subtilis, bacillus licheniformis, bacillus mucilaginosus and bacillus pumilus.
5. The carbon-based microbial composite bacterial powder according to claim 4, wherein the mass ratio of the bacillus subtilis to the bacillus licheniformis to the bacillus mucilaginosus to the bacillus pumilus is 1-3: 1-3.
6. The use of the carbon-based microbial composite bacterial powder according to any one of claims 1 to 5 in the treatment of livestock and poultry breeding sewage.
7. A carbon-based microbial composite bacterial agent, which is characterized by comprising the carbon-based microbial composite bacterial powder, a trace element material and a drying agent, wherein the carbon-based microbial composite bacterial powder is as defined in any one of claims 1 to 5;
the mass ratio of the carbon-based microorganism composite bacterial powder to the trace element material to the drying agent is 70-80: 2-15: 8-15.
8. The carbon-based microbial composite inoculant according to claim 7, wherein the trace element materials comprise zinc sulfate, magnesium sulfate and borax;
the desiccant comprises one or more of bentonite, silica gel, white carbon black and attapulgite.
9. The use of the carbon-based microbial composite inoculant according to claim 7 or 8 in the treatment of livestock and poultry breeding sewage.
10. The preparation method of the carbon-based microbial composite inoculum according to claim 7 or 8, which is characterized by comprising the following steps:
1) carrying out liquid state fermentation on microorganisms to obtain fermentation liquor, and adsorbing, drying and crushing the fermentation liquor by using a solid carrier to obtain carbon-based microorganism composite bacterial powder;
2) mixing the carbon-based microbial composite bacterial powder obtained in the step 1) with trace element materials and drying agents to obtain the carbon-based microbial composite bacterial agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911148942.3A CN110724683A (en) | 2019-11-21 | 2019-11-21 | Carbon-based microbial composite bacteria powder, microbial inoculum and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911148942.3A CN110724683A (en) | 2019-11-21 | 2019-11-21 | Carbon-based microbial composite bacteria powder, microbial inoculum and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110724683A true CN110724683A (en) | 2020-01-24 |
Family
ID=69225566
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911148942.3A Pending CN110724683A (en) | 2019-11-21 | 2019-11-21 | Carbon-based microbial composite bacteria powder, microbial inoculum and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110724683A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113816497A (en) * | 2021-10-28 | 2021-12-21 | 徐州工程学院 | Ecological floating bed for restoring aquaculture environment |
| CN114149287A (en) * | 2021-10-27 | 2022-03-08 | 哈尔滨师范大学 | Preparation method and application of solid microbial fertilizer |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102040307A (en) * | 2009-10-26 | 2011-05-04 | 陆振冈 | Sewage treatment system |
| CN104276874A (en) * | 2013-07-05 | 2015-01-14 | 北京微邦生物工程有限公司 | Microorganism-enzyme powdery composite biological preparation for promoting crop yield increase |
| CN107674853A (en) * | 2017-11-20 | 2018-02-09 | 上海市农业科学院 | A kind of complex microbial inoculum and its preparation method and application |
| CN108328737A (en) * | 2017-11-30 | 2018-07-27 | 河海大学 | A kind of Tiny ecosystem matrix, preparation method and its application based on artificial wet land reinforced processing breeding wastewater |
-
2019
- 2019-11-21 CN CN201911148942.3A patent/CN110724683A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102040307A (en) * | 2009-10-26 | 2011-05-04 | 陆振冈 | Sewage treatment system |
| CN104276874A (en) * | 2013-07-05 | 2015-01-14 | 北京微邦生物工程有限公司 | Microorganism-enzyme powdery composite biological preparation for promoting crop yield increase |
| CN107674853A (en) * | 2017-11-20 | 2018-02-09 | 上海市农业科学院 | A kind of complex microbial inoculum and its preparation method and application |
| CN108328737A (en) * | 2017-11-30 | 2018-07-27 | 河海大学 | A kind of Tiny ecosystem matrix, preparation method and its application based on artificial wet land reinforced processing breeding wastewater |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114149287A (en) * | 2021-10-27 | 2022-03-08 | 哈尔滨师范大学 | Preparation method and application of solid microbial fertilizer |
| CN113816497A (en) * | 2021-10-28 | 2021-12-21 | 徐州工程学院 | Ecological floating bed for restoring aquaculture environment |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102250768B (en) | Method for preparing enzyme and bacterium composite agent for treating sewage and sludge | |
| CN108689781A (en) | Biomass carbon base soil-repairing agent and preparation method thereof | |
| CN101691569B (en) | Bacillus cereus microbial preparation and method for treating nitrogenous waste water by using microbial preparation | |
| CN106148246A (en) | Purify compounding microbial inoculum of black and odorous water and preparation method thereof | |
| CN105582890A (en) | Preparation method and application of composite nano biomass charcoal material | |
| CN110982735A (en) | Biochar-based microbial inoculant and preparation method and application thereof | |
| CN108178341A (en) | A kind of solid microbe purification particle and preparation method thereof | |
| CN109762773A (en) | A kind of tree-shaped series bacillus, immobilized microbial inoculum and its application | |
| CN109455828A (en) | A kind of application method of immobilized microorganism in livestock breeding wastewater processing | |
| CN110791444A (en) | Pseudomonas stutzeri, composite microbial inoculum prepared from pseudomonas stutzeri and application of composite microbial inoculum | |
| CN110724683A (en) | Carbon-based microbial composite bacteria powder, microbial inoculum and preparation method and application thereof | |
| CN110591966B (en) | Environment deodorization composite microbial inoculant and preparation method thereof | |
| CN107460148A (en) | A kind of preparation method and application of bacillus subtilis bacterium powder | |
| CN106903151A (en) | The method that Composting repairing polluted soil is realized using stalk | |
| CN113388553A (en) | Ammonia nitrogen resistant composite microbial inoculum and application and preparation method thereof | |
| CN105820976A (en) | Sludge compost thermophilic microbial agent and application | |
| CN113736686A (en) | Desulfurization and ammonia removal microbial deodorant and preparation method thereof | |
| CN112342031A (en) | Soil composite modifier and application thereof | |
| CN102491534B (en) | Waste water treatment method using online bacterium throwing device | |
| CN105586331A (en) | Method of denitrified composite microbial inoculant | |
| CN105969698A (en) | Composite microbial inoculant for water purification | |
| CN105505810A (en) | Microbe composition and use thereof | |
| CN115491338A (en) | Chemical intermediate degrading strain and application thereof in wastewater treatment | |
| Sepehrnia et al. | Particle fractionation controls Escherichia coli release from solid manure | |
| JP3364438B2 (en) | Substance treatment method and substance treatment agent |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |