CN110687034A - Laser irradiation system of flow cytometer and flow cytometer - Google Patents
Laser irradiation system of flow cytometer and flow cytometer Download PDFInfo
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- CN110687034A CN110687034A CN201810732213.1A CN201810732213A CN110687034A CN 110687034 A CN110687034 A CN 110687034A CN 201810732213 A CN201810732213 A CN 201810732213A CN 110687034 A CN110687034 A CN 110687034A
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- 230000009286 beneficial effect Effects 0.000 abstract description 5
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- 230000005693 optoelectronics Effects 0.000 description 4
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
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Abstract
A laser irradiation system for a flow cytometer and a flow cytometer using the same, wherein propagation light paths of respective laser irradiation mechanisms of the laser irradiation system extend independently from different directions to a flow cell, and the sizes of focused spots irradiated to the flow cell by the respective laser irradiation mechanisms are substantially the same by respective optical components. Because the system does not need to combine a plurality of laser light paths into the same light path, the use of a dichroic mirror can be omitted, and the corresponding structural arrangement is saved, so that the laser irradiation system has smaller volume and is more beneficial to the miniaturization design of a flow cytometer.
Description
Technical Field
The present application relates to a flow cytometer, and more particularly, to a laser irradiation system for a flow cytometer.
Background
In flow cytometry, multiple Laser Diode (LD) illuminations are required for different fluorescence excitation and detection. Generally, one uses several dichroic mirrors to combine multiple laser beams and adjusts the laser beam and spot position by adjusting the pitch of the frame and rotating the dichroic mirrors left and right.
The main disadvantage of this optical path system is that the related devices have a plurality of structures, and especially under the condition of multi-path laser irradiation, the optical path system needs to adopt a responsible structure to combine the beams of the multi-path laser, so that the optical path system not only has a large volume and is not beneficial to the miniaturization design of the flow cytometer, but also has a plurality of parts, the related processes and tools are complicated, and the reliability, the pointing stability of the beams and the power stability are also deteriorated.
Disclosure of Invention
The application provides a novel laser irradiation system of a flow cytometer and the flow cytometer adopting the laser irradiation system, which are used for replacing the existing optical path system.
According to an aspect of the present application, in one embodiment, a laser irradiation system for a flow cytometer includes at least two laser irradiation mechanisms, where the laser irradiation mechanisms include a laser light source and an optical component, the optical component causes laser light emitted from the laser light source to propagate according to a set propagation light path, the propagation light path of each laser irradiation mechanism extends from different directions to a flow chamber, and the size of a focused light spot irradiated by each laser irradiation mechanism to the flow chamber is substantially the same.
As a further improvement of the laser irradiation system, all the laser irradiation mechanisms are disposed in the same plane.
As a further improvement of the laser irradiation system, the laser light sources of the laser irradiation mechanisms are arranged on the same circumference with the center of the flow chamber as the center, and the distances from the laser light sources to the flow chamber are approximately the same.
As a further improvement of the laser irradiation system, the laser light sources of the respective laser irradiation mechanisms are arranged substantially in a fan shape.
As a further improvement of the laser irradiation system, the optical components of each laser irradiation mechanism are arranged from the corresponding laser light source to the position of the flow chamber.
As a further improvement of the laser irradiation system, the laser irradiation system further comprises a laser power detection mechanism, the optical assembly comprises an optical filter, the laser power detection mechanism comprises a photoelectric element used for receiving light rays reflected by the optical filter, the optical filter is arranged in an inclined manner, and the photoelectric element is arranged on a reflection light path of the optical filter.
As a further improvement of the laser irradiation system, the inclination angle a of the optical filter is more than or equal to 5 degrees and less than or equal to 20 degrees.
As a further improvement of the laser irradiation system, the filter inclination angle a is 9 °.
As a further improvement of the laser irradiation system, the laser irradiation mechanism further includes a first support, the optical assembly further includes a collimating mirror, the laser light source and the collimating mirror are mounted on the first support, the optical filter is disposed on the laser path, a surface of the optical filter facing the laser incident direction is obliquely disposed downward, and the photoelectric element is also mounted on the first support.
As a further improvement of the laser irradiation system, the optical assembly includes an optical filter, a collimating mirror and a focusing mirror, and the collimating mirror and the focusing mirror are used for enabling the laser emitted by the corresponding laser light source to form a focusing spot meeting requirements in the flow chamber.
As a further improvement of the laser irradiation system, the optical filter is disposed between the focusing mirror and the laser light source.
As a further improvement of the laser irradiation system, the focusing mirror is arranged between the optical filter and the flow chamber, so that the laser transmitted from the optical filter can enter the focusing mirror, be focused by the focusing mirror and then be emitted to the flow chamber.
According to an aspect of the present application, there is provided in one embodiment a flow cytometer comprising a flow cell, and further comprising a laser illumination system as described in any of the above, wherein propagation optical paths of the respective laser illumination mechanisms extend from different directions toward the flow cell.
According to the laser irradiation system of the above embodiment, the propagation light paths of the laser irradiation mechanisms included therein extend independently from different directions toward the flow cell, and the sizes of the focused spots irradiated to the flow cell by the laser irradiation mechanisms are ensured to be substantially the same by the respective optical components. Because the system does not need to combine a plurality of laser light paths into the same light path, the use of a dichroic mirror can be omitted, and the corresponding structural arrangement is saved, so that the laser irradiation system has smaller volume and is more beneficial to the miniaturization design of a flow cytometer.
Drawings
FIG. 1 is a schematic layout of laser irradiation mechanisms according to an embodiment of the present application;
FIG. 2 is a schematic diagram of another layout of laser irradiation mechanisms according to an embodiment of the present application;
FIG. 3 is a schematic structural diagram of laser irradiation mechanisms according to an embodiment of the present application;
FIG. 4 is a schematic diagram of an obliquely arranged optical filter according to an embodiment of the present disclosure;
FIG. 5 is a schematic diagram of an embodiment of an optical filter and a laser source;
fig. 6 is a schematic structural diagram of a single-pass laser irradiation mechanism according to an embodiment of the present application.
Detailed Description
The present invention will be described in further detail with reference to the following detailed description and accompanying drawings. Wherein like elements in different embodiments are numbered with like associated elements. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.
Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. Also, the various steps or actions in the method descriptions may be transposed or transposed in order, as will be apparent to one of ordinary skill in the art. Thus, the various sequences in the specification and drawings are for the purpose of describing certain embodiments only and are not intended to imply a required sequence unless otherwise indicated where such sequence must be followed.
The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings).
The first embodiment is as follows:
the embodiment provides a laser irradiation system of a flow cytometer, which is used for providing at least two paths of irradiation laser to excite and detect different fluorescence.
Referring to fig. 1, the laser irradiation system includes at least two laser irradiation mechanisms 100, and a three laser irradiation mechanism 100 is illustrated as an example. The laser irradiation mechanism 100 includes a laser light source 110 and an optical unit 120, and the optical unit 120 propagates laser light emitted from the laser light source 110 along a predetermined propagation optical path. The propagation light paths of the laser irradiation mechanisms 100 extend from different directions to the flow chamber, and the propagation light paths formed by the laser irradiation mechanisms 100 are performed in the respective directions, so that beam combination is not required.
The optical assembly 120 is used for optically processing each laser, and may include, for example, a light shaping component, and through the action of the light shaping component, the size of the focused light spot irradiated to the flow chamber by each laser irradiation mechanism 100 is substantially the same, so as to ensure the excitation and detection of various fluorescent materials in the flow chamber by the laser.
The system does not need to combine multiple laser light paths into the same light path (beam combination), so that the use of a dichroic mirror can be omitted, and corresponding structural arrangement is saved, so that the laser irradiation system has a smaller volume and is more beneficial to the miniaturization design of a flow cytometer. The system has fewer parts, so the structure is simpler and more reliable, and the installation is convenient.
The laser irradiation mechanisms 100 may be distributed in a plane or space, and referring to fig. 1 and 2, in one embodiment, all the laser irradiation mechanisms 100 are disposed in the same plane. Further, the laser irradiation mechanism 100 may be located on the same plane as the irradiated portion of the flow cell 200. Here, the laser irradiation mechanisms 100 are disposed on the same plane, so that the flow cell 200 is irradiated with the laser irradiation mechanisms 100, and the structural design is also easier.
Referring to fig. 1 and 2, in an embodiment, the laser sources 110 of the laser irradiation mechanisms 100 are disposed on the same circumference around the flow chamber 200, and the distances from the laser sources 110 to the flow chamber 200 are substantially the same. For example, they may be distributed in a fan shape (as shown in fig. 1) or in a triangular shape (as shown in fig. 2). This arrangement simplifies the structural design of the laser irradiation mechanism 100, and the positions of the laser irradiation mechanisms 100 are substantially the same as the parameters of the optical assembly 120, which facilitates the design and assembly.
Of course, in some other embodiments, the distance from each laser source 110 to the flow cell 200 may be set to be different, and then the parameters and the position of the optical assembly 120 are adjusted to ensure that the sizes of the focused light spots irradiated to the flow cell 200 by each laser irradiation mechanism 100 are substantially the same.
Referring to fig. 1 and 3, in an embodiment, the laser light sources 110 of each laser irradiation mechanism 100 are arranged in a substantially fan shape. The Laser light source 110 may employ a Laser Diode (LD). This arrangement makes it possible to concentrate the laser irradiation mechanisms 100 in a certain area, thereby reducing the space occupied by the laser irradiation system, making the structure more compact and more advantageous for miniaturization.
The optical components 120 of each laser irradiation mechanism 100 are arranged from the corresponding laser light source 110 to the position of the flow chamber 200, so that the laser light emitted from the laser light source 110 is irradiated onto the flow chamber 200 along a straight line.
On the other hand, the power of the laser is monitored in a common laser irradiation system, and generally, a glass plate arranged at an inclination angle of 45 degrees is placed at the outlet of the laser, and is matched with a photodiode to monitor the power of the laser (some photodiodes are placed behind a dichroic mirror for power monitoring), and a filter is added in front of the laser diode to filter stray light in the laser and further control the emission spectrum of the LD. The detection mode has more related devices and adjustment structures, particularly under the condition of multi-path laser irradiation, the system has larger volume, is not beneficial to the miniaturization design of the flow cytometer, has more parts, more related processes and tools, and also has poor reliability, pointing stability of light beams and power stability.
In view of the above, referring to fig. 3-5, in one embodiment of the present application, the optical assembly 120 includes an optical filter 121, and a laser power detection mechanism is provided, the laser power detection mechanism includes an optoelectronic device 130 for receiving light reflected by the optical filter 121 and other corresponding components, the optoelectronic device 130 may employ a photodiode, and the other corresponding components are not described in detail herein. The filter 121 is disposed obliquely, and the photoelectric element 130 is disposed on a reflection light path of the filter 121. The filter 121 placed obliquely reflects a part of light to the photodiode while filtering out stray light, and is used for LD power detection.
In the embodiment, the optical filter 121 is directly used for reflecting a part of laser to realize the detection of the power of the laser, so that the original glass plate for reflection can be saved, the laser irradiation system is more compact, the structure is simpler, the cost is lower, and the assembly is more convenient.
The optical filter 121 is disposed obliquely relative to the optical axis of the laser propagation light path, and in an embodiment of the present embodiment, the inclination angle a of the optical filter 121 is greater than or equal to 5 ° and less than or equal to 20 °. Referring to fig. 4, the inclination angle a is an angle a between the filter 121 and the vertical direction.
Preferably, the filter 121 is inclined at an angle a of 9 °, which facilitates the arrangement of the optoelectronic device 130, for example, the optoelectronic device 130 and the laser source 110 are arranged on the same bracket, thereby further reducing the space occupied by the system.
Further, the optical assembly 120 may select corresponding components according to actual requirements, and may include a light filtering assembly and a light shaping assembly, for example. Referring to fig. 5 and 6, the optical assembly 120 further includes a collimating mirror 122 and a focusing mirror 123 in addition to the filter 121, the filter 121 is used for filtering out stray light, and the collimating mirror 122 and the focusing mirror 123 are used for enabling the laser emitted by the corresponding laser source 110 to form a focusing spot meeting requirements in the flow chamber 200.
The laser irradiation mechanism 100 may include a first frame 111, the laser source 110 (in this embodiment, a single LD tube) and the collimator lens 122 are mounted on the first frame 111, and the photoelectric element 130 is also mounted on the first frame 111. The filter 121 is disposed on the laser path, and one surface of the filter 121 facing the laser incident direction is disposed obliquely downward so that the laser reflected therefrom can be irradiated onto the photoelectric element 130. Thus, a plurality of components, such as the laser light source 110, the collimating mirror 122 and the photoelectric element 130, can be integrated into one first bracket 111, further improving the compactness of the structure.
The collimating mirror 122 may be fixedly mounted on the first bracket 111 by a collimating lens barrel 1221. With continued reference to fig. 5 and 6, in one embodiment, the filter 121 is disposed between the focusing mirror 123 and the laser source 110. A filter holder 1211 may be disposed at a position spaced apart from the first holder 111, and the filter 121 is mounted on the holder corresponding to the wavelength filter 121.
Referring to fig. 5 and 6, the focusing mirror 123 is disposed between the optical filter 121 and the flow chamber 200, so that the laser light transmitted from the optical filter 121 can enter the focusing mirror 123, be focused by the focusing mirror 123, and then be emitted to the flow chamber 200. Specifically, the focusing mirror 123 focuses the quasi-parallel light emitted from the laser source 110 in the channel of the flow cell 200, and the position of the focusing spot on the flow cell 200 is finely adjusted by adjusting the position of the focusing mirror 123. Preferably, the focusing mirror 123 may be a cylindrical mirror, a prism, or an optical wedge. The position of the light spot on the flow cell 200 is adjusted by the focusing mirror 123, and the dichroic mirror is omitted. The focusing lens 123 may be mounted in a focusing lens barrel 1232, the focusing lens barrel 1232 being mounted on a separately provided focusing lens holder 1231, the focusing lens holder 1231 being provided between the optical filter 121 and the flow cell 200.
The collimating lens 122 can be a corresponding laser shaping lens, such as a spherical positive lens, an aspherical lens, a cylindrical lens, a prism, or a wedge.
Example two:
this embodiment provides a flow cytometer comprising a flow cell and a laser illumination system as shown in any of the above embodiments.
The propagation light paths of the laser irradiation mechanisms in the laser irradiation system extend to the flow chamber from different directions, so that different fluorescence in the flow chamber is excited and detected.
The laser irradiation system can omit the prior dichroic mirror and a glass plate for reflection, so that the laser irradiation system is more compact, the volume of the whole flow cytometer can be reduced, and the miniaturization is realized.
The present invention has been described in terms of specific examples, which are provided to aid understanding of the invention and are not intended to be limiting. For a person skilled in the art to which the invention pertains, several simple deductions, modifications or substitutions may be made according to the idea of the invention.
Claims (13)
1. The laser irradiation system of the flow cytometer is characterized by comprising at least two laser irradiation mechanisms, wherein each laser irradiation mechanism comprises a laser light source and an optical component, the optical component enables laser emitted by the laser light source to propagate according to a set propagation light path, the propagation light paths of the laser irradiation mechanisms extend to a flow chamber from different directions, and the sizes of focused light spots irradiated to the flow chamber by the laser irradiation mechanisms are approximately the same.
2. The laser irradiation system according to claim 1, wherein all the laser irradiation mechanisms are disposed in the same plane.
3. The laser irradiation system according to claim 2, wherein the laser light sources of the respective laser irradiation mechanisms are arranged on the same circumference with the center of the flow cell as a center, and distances from the respective laser light sources to the flow cell are substantially the same.
4. The laser irradiation system according to claim 3, wherein the laser light sources of the respective laser irradiation mechanisms are arranged in a substantially fan shape.
5. The laser irradiation system according to claim 3, wherein the optical components of each laser irradiation mechanism are arranged from the corresponding laser light source toward the position of the flow cell.
6. The laser irradiation system according to any one of claims 1 to 5, further comprising a laser power detection mechanism, wherein the optical assembly comprises an optical filter, the laser power detection mechanism comprises an electro-optical element for receiving light reflected by the optical filter, the optical filter is disposed obliquely, and the electro-optical element is disposed in a reflected light path of the optical filter.
7. The laser irradiation system according to claim 6, wherein the filter inclination angle a is 5 ° or more and 20 ° or less.
8. The laser irradiation system according to claim 6, wherein the filter inclination angle a is 9 °.
9. The laser irradiation system according to any one of claims 6 to 8, wherein the laser irradiation mechanism further comprises a first support, the optical assembly further comprises a collimator lens, the laser light source and the collimator lens are mounted on the first support, the optical filter is disposed in a laser path, a surface of the optical filter facing a laser light incidence direction is disposed obliquely downward, and the photoelectric element is also mounted on the first support.
10. The laser irradiation system according to any one of claims 1 to 5, wherein the optical assembly comprises an optical filter, a collimating mirror and a focusing mirror, and the collimating mirror and the focusing mirror are configured to enable the laser light emitted from the corresponding laser light source to form a focused spot meeting requirements in the flow chamber.
11. The laser irradiation system according to claim 10, wherein the filter is disposed between the focusing mirror and the laser light source.
12. The laser irradiation system according to claim 10, wherein the focusing mirror is disposed between the optical filter and the flow cell, so that the laser light transmitted from the optical filter can be incident on the focusing mirror, focused by the focusing mirror, and then emitted to the flow cell.
13. A flow cytometer comprising a flow cell, and further comprising a laser illumination system according to any of claims 1 to 12, wherein the propagation paths of the laser illumination mechanisms in the laser illumination system extend from different directions toward the flow cell.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810732213.1A CN110687034A (en) | 2018-07-05 | 2018-07-05 | Laser irradiation system of flow cytometer and flow cytometer |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810732213.1A CN110687034A (en) | 2018-07-05 | 2018-07-05 | Laser irradiation system of flow cytometer and flow cytometer |
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| CN110687034A true CN110687034A (en) | 2020-01-14 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114488547A (en) * | 2022-03-07 | 2022-05-13 | 中国科学院苏州生物医学工程技术研究所 | Beam shaping system for flow cytometer |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2667747Y (en) * | 2003-11-26 | 2004-12-29 | 上海冠威光电有限公司 | Enclosed micro green light laser |
| CN101278829A (en) * | 2008-05-26 | 2008-10-08 | 上海理工大学 | Portable in vivo flow cytometry |
| US20080317076A1 (en) * | 2007-06-21 | 2008-12-25 | Fujitsu Limited Of Kawasaki, Japan | Controlling output wavelength of a light source |
| CN202066590U (en) * | 2011-02-22 | 2011-12-07 | 闫妍 | Laser power detecting apparatus |
| CN104155242A (en) * | 2014-07-24 | 2014-11-19 | 太仓能健生物技术有限公司 | Light path device of fluid analysis equipment |
| CN104483254A (en) * | 2014-12-29 | 2015-04-01 | 中国科学院长春光学精密机械与物理研究所 | Multi-color multi-parameter portable flow cytometer |
| TW201715210A (en) * | 2015-10-23 | 2017-05-01 | 波若威科技股份有限公司 | Integrated power monitor with high directivity |
| CN107478612A (en) * | 2017-09-20 | 2017-12-15 | 安费诺(常州)连接***有限公司 | For detecting the sensor and method of filter laying dust |
-
2018
- 2018-07-05 CN CN201810732213.1A patent/CN110687034A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2667747Y (en) * | 2003-11-26 | 2004-12-29 | 上海冠威光电有限公司 | Enclosed micro green light laser |
| US20080317076A1 (en) * | 2007-06-21 | 2008-12-25 | Fujitsu Limited Of Kawasaki, Japan | Controlling output wavelength of a light source |
| CN101278829A (en) * | 2008-05-26 | 2008-10-08 | 上海理工大学 | Portable in vivo flow cytometry |
| CN202066590U (en) * | 2011-02-22 | 2011-12-07 | 闫妍 | Laser power detecting apparatus |
| CN104155242A (en) * | 2014-07-24 | 2014-11-19 | 太仓能健生物技术有限公司 | Light path device of fluid analysis equipment |
| CN104483254A (en) * | 2014-12-29 | 2015-04-01 | 中国科学院长春光学精密机械与物理研究所 | Multi-color multi-parameter portable flow cytometer |
| TW201715210A (en) * | 2015-10-23 | 2017-05-01 | 波若威科技股份有限公司 | Integrated power monitor with high directivity |
| CN107478612A (en) * | 2017-09-20 | 2017-12-15 | 安费诺(常州)连接***有限公司 | For detecting the sensor and method of filter laying dust |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114488547A (en) * | 2022-03-07 | 2022-05-13 | 中国科学院苏州生物医学工程技术研究所 | Beam shaping system for flow cytometer |
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