CN110669717A - Screening method of saccharomyces cerevisiae - Google Patents
Screening method of saccharomyces cerevisiae Download PDFInfo
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- CN110669717A CN110669717A CN201911207488.4A CN201911207488A CN110669717A CN 110669717 A CN110669717 A CN 110669717A CN 201911207488 A CN201911207488 A CN 201911207488A CN 110669717 A CN110669717 A CN 110669717A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/36—Adaptation or attenuation of cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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Abstract
The invention relates to a screening method of saccharomyces cerevisiae, which comprises the following steps: fixing the saccharomyces cerevisiae on a specific carrier, determining domestication parameters according to a set target to perform directional domestication on the saccharomyces cerevisiae, and breeding a target strain by combining a TTC double-layer color development plate after certain period of domestication. The method is simple to operate, the target strain is rapidly screened, the total amount of wastewater and energy consumption in the alcohol industry can be effectively reduced through optimization of the saccharomyces cerevisiae, and the development of the alcohol industry is powerfully promoted.
Description
Technical Field
The invention relates to a screening method of saccharomyces cerevisiae strains, in particular to screening of saccharomyces cerevisiae.
Background
Alcohol is currently entering into industrial products of national key development as a renewable strategic resource. The alcohol fermentation industry is also an industry with great pollution to the environment at present. Most of the wastewater generated by the traditional fermentation method is discharged into natural water after being treated, and although various indexes such as COD (chemical oxygen demand) and the like in the discharged water after being treated reach the standard, the difference of the discharged water and the natural water is still large. The alcohol fermentation industry is a bulk product production industry, the discharged water metering unit is ten thousand tons, and the pollution to natural water still exists.
Disclosure of Invention
The invention aims to breed a strain which can realize high-concentration fermentation and high-temperature resistant fermentation through directional domestication and natural mutagenesis of the strain, thereby reducing the consumption of water resources and energy resources and reducing the generation of wastewater.
The purpose of the invention can be realized by the following technical scheme:
a screening method of saccharomyces cerevisiae comprises the following steps:
(1) fixing the saccharomyces cerevisiae on a specific carrier, and determining domestication parameters according to a set target to perform directional domestication on the saccharomyces cerevisiae;
(2) and combining the domesticated bacterial liquid with a TTC double-layer color development plate to breed the target bacterial strain.
The specific carrier comprises active fibers and the like.
The set target refers to high concentration resistant culture medium, high temperature resistant fermentation or combination of the two.
The invention has the advantage of simple operation (1). It is only necessary to immobilize the strain to a carrier and then repeatedly inoculate the medium to stimulate the strain to proceed by the external environment. (2) And (5) rapidly screening the target strain. The yeast strains which grow fast and produce more wine can be rapidly judged by combining a TTC double-layer color development plate, and the excellent strains are rapidly bred. The method has less investment and quick response; through the optimization of the saccharomyces cerevisiae, the total amount of wastewater and energy consumption in the alcohol industry can be effectively reduced, and the development of the alcohol industry is powerfully promoted.
Detailed Description
The present invention will be described in further detail with reference to the following examples, which are provided only for illustrating the present invention and are not intended to limit the scope of the present invention.
Example 1 the invention takes active fiber as a carrier, fixes saccharomyces cerevisiae strains for high concentration resistant domestication, and adopts the following process steps: taking 6g of active fiber as a carrier, placing the active fiber into a 250ml triangular flask, inoculating an activated yeast strain, statically fixing for 24h, then inoculating a sterilized cassava mash culture medium, keeping the total sugar content of the cassava mash at 40g/100ml, statically domesticating at 35 ℃, ensuring that the cassava mash is shaken once every 8h, and still replacing by the cassava mash with the same concentration after domesticating for 48 h; after 90 batches of repeated domestication, screening the domesticated culture solution by adopting a TTC double-layer color development plate, selecting better 6 strains for fermentation comparison to obtain a strain, and fermenting the strain at 35 ℃ for 40h until the alcohol content reaches 18.5g/100 ml.
Example 2 the invention takes active fiber as a carrier, fixes saccharomyces cerevisiae strains for high concentration resistant domestication, and adopts the following process steps: taking 6g of active fiber as a carrier, placing the active fiber into a 250ml triangular flask, inoculating an activated yeast strain, statically fixing for 24h, then inoculating a sterilized cassava mash culture medium, keeping the total sugar content of the cassava mash at 40g/100ml, statically domesticating at 37 ℃, ensuring that the cassava mash is shaken once every 8h, and still replacing by the cassava mash with the same concentration after domesticating for 48 h; after 90 batches of repeated domestication, screening the domesticated culture solution by adopting a TTC double-layer color development plate, selecting better 6 strains for fermentation comparison to obtain a strain, and fermenting the strain at 37 ℃ for 52h until the alcohol content reaches 18.1g/100 ml.
The foregoing is directed to preferred embodiments of the present invention, other and further embodiments of the invention may be devised without departing from the basic scope thereof, and the scope thereof is determined by the claims that follow. However, any simple modification, equivalent change and modification of the above embodiments according to the technical essence of the present invention are within the protection scope of the technical solution of the present invention.
Claims (3)
1. The screening method of the saccharomyces cerevisiae is characterized by comprising the following steps:
(1) fixing the saccharomyces cerevisiae on a specific carrier, and determining domestication parameters according to a set target to perform directional domestication on the saccharomyces cerevisiae;
(2) and combining the domesticated bacterial liquid with a TTC double-layer color development plate to breed the target bacterial strain.
2. The method for screening Saccharomyces cerevisiae according to claim 1, wherein: the specific carrier described in step (1) comprises reactive fibers.
3. The method for screening Saccharomyces cerevisiae according to claim 1, wherein: the set target refers to high concentration resistant culture medium and/or high temperature resistant fermentation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911207488.4A CN110669717A (en) | 2019-11-30 | 2019-11-30 | Screening method of saccharomyces cerevisiae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911207488.4A CN110669717A (en) | 2019-11-30 | 2019-11-30 | Screening method of saccharomyces cerevisiae |
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| Publication Number | Publication Date |
|---|---|
| CN110669717A true CN110669717A (en) | 2020-01-10 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201911207488.4A Pending CN110669717A (en) | 2019-11-30 | 2019-11-30 | Screening method of saccharomyces cerevisiae |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105238842A (en) * | 2015-10-30 | 2016-01-13 | 南阳理工学院 | Biological acid adjustment method applied in thick mash alcoholic fermentation of cassava |
| CN107603898A (en) * | 2017-10-26 | 2018-01-19 | 中国科学院天津工业生物技术研究所 | The application of saccharomyces cerevisiae and its breeding mode and industrial fermentation production ethanol |
| CN109401989A (en) * | 2018-11-30 | 2019-03-01 | 吉林中粮生化有限公司 | A kind of acclimation method of an industrial strain of S.cerevisiae |
| CN109943555A (en) * | 2018-12-29 | 2019-06-28 | 南京工业大学 | Method for continuously preparing yeast wine by utilizing surface immobilization technology to perform alcohol fermentation |
-
2019
- 2019-11-30 CN CN201911207488.4A patent/CN110669717A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105238842A (en) * | 2015-10-30 | 2016-01-13 | 南阳理工学院 | Biological acid adjustment method applied in thick mash alcoholic fermentation of cassava |
| CN107603898A (en) * | 2017-10-26 | 2018-01-19 | 中国科学院天津工业生物技术研究所 | The application of saccharomyces cerevisiae and its breeding mode and industrial fermentation production ethanol |
| CN109401989A (en) * | 2018-11-30 | 2019-03-01 | 吉林中粮生化有限公司 | A kind of acclimation method of an industrial strain of S.cerevisiae |
| CN109943555A (en) * | 2018-12-29 | 2019-06-28 | 南京工业大学 | Method for continuously preparing yeast wine by utilizing surface immobilization technology to perform alcohol fermentation |
Non-Patent Citations (4)
| Title |
|---|
| 毛玉红: "《水生物处理新技术》", 30 April 2015, 中国铁道出版社 * |
| 王昌魁等: "耐高温耐高糖酵母的筛选与驯化", 《江西农业学报》 * |
| 胡晓冰等: "TTC法在筛选西瓜果酒酵母中的应用", 《酿酒科技》 * |
| ***: "《现代科学技术大众百科 技术卷》", 30 June 2001, 浙江教育出版社 * |
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