CN110669114B - Lanthionine precursor peptide amyA6, and preparation method and application thereof - Google Patents
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Abstract
The invention discloses a lantibiotic peptide precursor peptide amyA6, a preparation method and application thereof, and relates to the technical field of biology. The amino acid sequence of the lantibiotic precursor peptide amyA6 is shown in SEQ ID NO 1. The lantibiotic peptide precursor amyA6 is added in the fermentation process of the bacillus amyloliquefaciens, so that the antibacterial activity of the metabolite of the bacillus amyloliquefaciens can be obviously improved, and the duration of the antibacterial activity is more than 4 times of the original antibacterial activity.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a lantibiotic peptide precursor amyA6, a preparation method thereof and application thereof in the process of improving the antibacterial activity of a bacillus amyloliquefaciens metabolite.
Background
In agricultural production today, plant diseases are one of the major causes affecting crop yield. The traditional method of spreading chemical pesticides is mostly adopted at present to solve the problem of agricultural diseases. The method not only causes pollution to the environment, but also harms other animals, plants and human health. In recent years, researchers begin to adopt biological control methods to treat plant diseases, and biological pesticides have a series of advantages of high efficiency, environmental protection, no drug resistance and the like. Therefore, the search for new highly effective biological control means to solve plant diseases has been largely delayed.
The current research on biocontrol strains mainly includes biocontrol bacteria (bacillus, agrobacterium, pseudomonas) and biocontrol fungi (trichoderma). Wherein, the bacillus amyloliquefaciens of the bacillus has better inhibiting effect on various plant diseases and has broad-spectrum antibacterial activity. A large number of researches show that the antibacterial substance derived from the bacillus amyloliquefaciens has good control effect on fusarium dry rot, early blight, gray mold and other plant diseases.
At present, the drug resistance of germs is a big problem in the process of preventing and treating plant diseases or medical clinical treatment. The bacillus can generate various metabolites, the lantibiotic peptide serving as an antibacterial peptide has a dual action mechanism compared with the traditional antibiotics, can inhibit various bacteria and fungi (multidrug-resistant bacteria) and is not easy to generate drug resistance, and the lantibiotic peptide is expected to be applied to the aspects of agricultural production, clinical medicine and the like.
Disclosure of Invention
The invention aims to solve the technical problem of providing a lantibiotic peptide precursor amyA6 and a preparation method and application thereof, wherein the lantibiotic peptide precursor amyA6 is added in the fermentation process of bacillus amyloliquefaciens, so that the antibacterial activity of the metabolite of the bacillus amyloliquefaciens can be obviously improved, and the duration of the antibacterial activity is more than 4 times of the original antibacterial activity.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows: a lantibiotic precursor peptide, amyA6, having the amino acid sequence of amyA6 shown in SEQ ID NO 1.
The preparation method of the lantibiotic precursor peptide amyA6 comprises the following steps: the lantibiotic peptide precursor amyA6 is obtained by heterologous expression or artificial synthesis of the lantibiotic peptide precursor amyA6 gene with a specific gene sequence in Escherichia coli.
The above-mentioned lantibiotic precursor peptide amyA6 gene was derived from Bacillus amyloliquefaciens S499. Extracting genome of Bacillus amyloliquefaciens S499 as a template, taking a primer A6-S, A6-A as an upstream primer and a downstream primer (SEQ ID NO: 3; SEQ ID NO:4), and carrying out PCR amplification to obtain the lanthionine precursor peptide amyA6 gene fragment.
The gene sequence of the above-mentioned lantibiotic precursor peptide amyA6, amyA6, is shown in SEQ ID NO 2.
The heterologous expression method of the escherichia coli comprises the following steps:
(1) construction and transformation of the recombinant vector for the lantibiotic precursor peptide amyA 6: the lantibiotic precursor peptide amyA6 gene fragment is constructed on a PET-28a vector by a gene recombination method. The recombinant vector was transformed into E.coli BL21(DE3) by heat shock transformation.
(2) Induction of expression of the lantibiotic precursor peptide amyA 6: BL21 expression strain is cultured in liquid LB culture medium, heterologous expression of lanthionine precursor peptide amyA6 protein is induced by adding IPTG, the thalli is re-suspended by 8M urea solution, and then the thalli is broken by ultrasonic, and the lanthionine precursor peptide amyA6 is released. The supernatant was collected by centrifugation, dialyzed for three days using a dialysis bag with a pore size of 1000Da and finally freeze-dried to powder of the lantibiotic precursor peptide amyA 6.
The application of the lantibiotic precursor peptide amyA6 is as follows: application of lantibiotic precursor peptide amyA6 in the process of improving the antibacterial activity of a bacillus amyloliquefaciens metabolite.
Preferably, the lantibiotic precursor peptide amyA6 is added for the following period: the fermentation of the bacillus amyloliquefaciens starts to the end of the logarithmic phase of growth.
Preferably, the lantibiotic precursor peptide amyA6 is added in an amount of: 0.001-10mg/L of lanthionine precursor peptide amyA6 is added into the fermentation liquor of bacillus amyloliquefaciens.
Specifically, the application comprises the following steps: culturing (fermenting) Bacillus amyloliquefaciens in liquid NB culture medium for 12 hours to logarithmic phase, taking out the shaking table, adding 0.001-10mg/L lanthionine precursor peptide amyA6 into the fermentation liquid, continuing fermentation culture, centrifuging after 24 hours, and collecting supernatant.
Adopt the produced beneficial effect of above-mentioned technical scheme to lie in:
firstly, the lantibiotic peptide precursor peptide amyA6 is added into the bacillus amyloliquefaciens fermentation liquor, so that the bacteriostatic activity of the lantibiotic peptide precursor peptide amyA6 can be obviously enhanced. The results show that the diameter of the inhibition zone of the experimental group is enlarged by more than 2 times compared with the control group, and the existence time of the inhibition zone can be prolonged by more than 4 times compared with the control group. Secondly, the method has low production cost, short production period, simple process and high yield. Finally, the method adopts biological control to treat plant diseases, and does not cause harm to human bodies, animals, plants and ecological environment. Therefore, the method has wide application prospect.
Detailed Description
The present invention will be described in further detail with reference to specific embodiments.
Experimental materials and reagents required in the first and following examples
1. Strains and vectors:
bacillus amyloliquefaciens S499 was purchased from the bacterial collection center of the Council Committee for culture of microorganisms (BCCM/LMG) and designated LMG 29676. Coli BL21(DE3) was purchased from TransGen Biotech, Inc., Beijing Korea. pET-28a vectors were purchased from Tiannze Biotechnology Inc. (TIANDZ).
2. Enzymes and other biochemical reagents:
KOD-Plus polymerase was purchased from Shanghai Gentianbio Ltd (TOYOBO). The TIANGEN bacterial genome DNA extraction kit is a product of Tiangen Biochemical technology Co. The Axygen gel recovery kit is a product of American Seikagan company. The Vazyme kit is a product of Nanjing Novozam Biotech Co.
3. Unless otherwise indicated, the biochemical techniques used in the present invention are conventional in the art.
II, the formula of the culture medium required in the following examples:
LB culture medium: 10g of tryptone, 5g of NaCl and 5g of yeast extract, adding deionized water to reach the constant volume of 1000ml, and adjusting the pH value to 7.0-7.2.
NB medium: 10g of peptone, 5g of NaCl, 5g of beef extract and 10g of glucose, adding deionized water to reach the constant volume of 1000ml, and adjusting the pH value to 7.0-7.2.
PDA culture medium: 200g of potatoes and 20g of glucose, and adding deionized water to the volume of 1000 ml.
The culture medium is liquid culture medium, and 12g agar is added into corresponding solid culture medium.
Example 1 Source and selection procedure for the lanthionine precursor peptide amyA6
The lanthionine precursor peptide amyA6 gene was derived from Bacillus amyloliquefaciens S499.
The whole genome of Bacillus amyloliquefaciens S499 was extracted using the TIANGEN bacterial genomic DNA extraction kit.
Primer 5.0 was used to design lantibiotic precursor peptide amyA6 primers, with homologous fragments at both ends of pET-28a added as homology arms at both ends of the precursor peptide sequence (A6-S: CTTTAAGAAGGAGATATACCATGAAAAAGAACTTCAGCGCG, SEQ ID NO: 3; A6-A: CCTTTCGGGCTTTGTTATACTTGCCTTTGCGGAC, SEQ ID NO: 4).
The lantibiotic precursor peptide amyA6 gene fragment was PCR amplified. The PCR reaction was carried out using KOD-Plus polymerase with genomic DNA of Bacillus amyloliquefaciens S499 as a template and primers A6-S, A6-A as upstream and downstream primers, and PCR was carried out to obtain the lantibiotic peptide precursor peptide amyA6 gene fragment.
The gene sequence of the precursor peptide amyA6 is: ATGAAAAAGAACTTCAGCGCGTTGTATGAAACATCAGAACAAGAATTAAAAGAGCTTGTTGGAGGGCAAAACTCAGTATCAATAACAACATATCCAATCACAAATCATATTTGTCCAACTATCACAGTAGGCTGCGCATGTCCGCAAAGGCAAGTATAA are provided. Shown as SEQ ID NO. 2.
The PCR reaction system is as follows: DNA template, about 100 ng; 10 μ M of upstream and downstream primers, each 1.5 μ L; 10 × Buffer, 5 μ L; 2Mm dNTPs, 5 μ L; 25mM MgSO4, 2. mu.L; KOD-Plus, 1. mu.L, water was added to make volume 50. mu.L. The PCR reaction program is: pre-denaturation at 98 ℃ for 2 min; denaturation at 98 ℃ for 30 s; tm-5 ℃, and renaturation is 40 s; extending for 1min at 68 ℃; stretching at 68 deg.C for 10 min; and preserving at 4 ℃.
EXAMPLE 2 heterologous expression of E.coli
(1) Construction of precursor peptide Gene expression vector
A precursor peptide Primer and a pET-28a vector sequence specific Primer are designed by using Primer 5.0, and homologous fragments at two ends of pET-28a are added at two ends of the precursor peptide sequence to be used as homologous arms. PCR is carried out on the precursor peptide gene fragment and the pET-28a carrier fragment, a PCR amplification product is verified through 1% agarose gel electrophoresis, and an Axygen gel recovery kit is used for recovering a target band. The purified precursor peptide gene fragment and the pET-28a vector fragment are connected by ligase in a Vazyme kit to construct a recombinant vector. The recombinant vector was transformed into E.coli BL21(DE3) by heat shock transformation.
(2) Obtaining of inducible expression precursor peptide protein
The precursor peptide protein is obtained by adopting a molecular biology technology heterologous expression mode. BL21 expression strain is cultured in liquid LB culture medium, heterologous expression of precursor peptide protein is induced by adding IPTG (Isopropyl Thiogalactoside, isoproyl beta-D-Thiogalactoside), the thallus is re-suspended by 8M urea solution, and the thallus is broken by ultrasonic wave to release the precursor peptide. The supernatant was collected by centrifugation, dialyzed for three days using a dialysis bag with a pore size of 1000Da and finally freeze-dried to powder of the lantibiotic precursor peptide amyA 6.
The amino acid sequence of the precursor peptide amyA6 is:
Met-Lys-Lys-Asn-Phe-Ser-Ala-Leu-Tyr-Glu-Thr-Ser-Glu-Gln-Glu-Leu-Lys-Glu-Leu-Val-Gly-Gly-Gln-Asn-Ser-Val-Ser-Ile-Thr-Thr-Tyr-Pro-Ile-Thr-Asn-His-Ile-Cys-Pro-Thr-Ile-Thr-Val-Gly-Cys-Ala-Cys-Pro-Gln-Arg-Gln-Val. Shown as SEQ ID NO. 1.
Example 3
The application of the lantibide precursor peptide amyA6 in the process of improving the antibacterial activity of the metabolite of the bacillus amyloliquefaciens comprises the following steps:
(1) fermentation of bacillus amyloliquefaciens:
experimental groups: culturing (fermenting) bacillus amyloliquefaciens in a liquid NB culture medium for 12 hours to a logarithmic phase, taking out a shaking table, adding 0.001-10mg/L lanthionine precursor peptide amyA6 into a fermentation liquid, continuing fermentation culture, and centrifuging after 24 hours to collect a supernatant (supernatant 1);
control group: culturing (fermenting) bacillus amyloliquefaciens in a liquid NB medium for 12 hours to the logarithmic phase, taking out the shaking table simultaneously with the experimental group, adding no lantibiotic precursor peptide amyA6, continuing the fermentation culture, and centrifuging after 24 hours to collect the supernatant (supernatant 2).
(2) Plate confrontation test:
carrying out a plate confrontation experiment by taking Botrytis cinerea, Fusarium sambucinum and Alternaria solani as indicator bacteria;
experimental groups: preparing an indicator bacterium suspension, pouring the indicator bacterium suspension into a preheated and melted solid PDA culture medium, uniformly mixing, and pouring 30mL of the indicator bacterium suspension into each flat plate in a quantitative manner; after the flat plate is solidified, punching by using a puncher with the diameter of 8 mm; 100uL of prepared fermentation supernatant liquid 1 is added into the holes, and finally the mixture is placed in a constant temperature incubator at 26 ℃ for culturing for several days, and the result is observed.
Control group: 100uL of the prepared fermentation broth "supernatant 2" was added to another well as a control, and the mixture was cultured in a constant temperature incubator at 26 ℃ for several days, and the results were observed.
Example 4 application of lanthionine precursor peptide amyA6 in improving antibacterial Activity of metabolites of Bacillus amyloliquefaciens
Bacillus amyloliquefaciens was cultured in NB medium until logarithmic phase, and 0.1mg/L of lanthionine precursor peptide amyA6 was added to the fermentation broth, which was used as experimental group (supernatant 1). The control was the fermentation broth without the addition of the lantibiotic precursor peptide amyA6 (supernatant 2). And collecting the fermentation broth supernatant after the culture is finished. The plate confrontation experiment is carried out by taking Botrytis cinerea, Fusarium sambucinum and Alternaria solani as indicator bacteria. The prepared bacterial suspension is poured into a preheated and melted solid PDA culture medium, mixed evenly and placed on plates, and 30mL of the prepared bacterial suspension is poured into each plate quantitatively. After the plates are solidified, a puncher with the diameter of 8mm is used for punching, two holes are punched on each plate, 100uL of the prepared fermentation broth supernatant 1 (experimental group) is added into one hole, 100uL of the prepared fermentation broth supernatant 2 (control group) is added into the other hole, and finally the plates are placed in a constant-temperature incubator at 26 ℃ for culturing for several days, and the results are observed. The results show that the diameter of the inhibition zone of the experimental group is enlarged by more than 2 times compared with the control group, and the existence time of the inhibition zone can be prolonged by more than 4 times compared with the control group.
EXAMPLES 5-8 results of applications of lanthionine precursor peptide amyA6 at various addition levels
Different addition amounts of the precursor peptides are set, and the operation is carried out according to the embodiment 3, and the influence of the different addition amounts of the precursor peptides on the activity of the bacillus amyloliquefaciens for inhibiting the botrytis cinerea, the fusarium sambucinum and the alternaria solani is observed through experiments.
TABLE 1 Effect of different amounts of precursor peptide on the bacteriostatic activity of Bacillus amyloliquefaciens
The result shows that the addition of the lantibiotic peptide precursor peptide amyA6 can obviously improve the bacteriostatic activity of the bacillus amyloliquefaciens metabolite and prolong the duration of the bacteriostatic effect.
Sequence listing
<110> institute of biological research of academy of sciences of Hebei province
<120> lanthionine precursor peptide amyA6, and preparation method and application thereof
<130> 2019
<160> 4
<170> SIPOSequenceListing 1.0
<210> 1
<211> 52
<212> PRT
<213> Artificial Sequence (Artificial Sequence)
<400> 1
Met Lys Lys Asn Phe Ser Ala Leu Tyr Glu Thr Ser Glu Gln Glu Leu
1 5 10 15
Lys Glu Leu Val Gly Gly Gln Asn Ser Val Ser Ile Thr Thr Tyr Pro
20 25 30
Ile Thr Asn His Ile Cys Pro Thr Ile Thr Val Gly Cys Ala Cys Pro
35 40 45
Gln Arg Gln Val
50
<210> 2
<211> 159
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 2
atgaaaaaga acttcagcgc gttgtatgaa acatcagaac aagaattaaa agagcttgtt 60
ggagggcaaa actcagtatc aataacaaca tatccaatca caaatcatat ttgtccaact 120
atcacagtag gctgcgcatg tccgcaaagg caagtataa 159
<210> 3
<211> 41
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 3
ctttaagaag gagatatacc atgaaaaaga acttcagcgc g 41
<210> 4
<211> 34
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 4
cctttcgggc tttgttatac ttgcctttgc ggac 34
Claims (5)
1. A lantibiotic precursor peptide, amyA6, comprising: the amino acid sequence of the lantibiotic precursor peptide amyA6 is shown in SEQ ID NO: 1.
2. The method of claim 1, wherein the preparation of the lantibiotic precursor peptide amyA6 comprises: the lantibiotic precursor peptide amyA6 was obtained by heterologous expression in E.coli or by artificial synthesis.
3. The use of the lantibiotic precursor peptide amyA6 according to claim 1, wherein: application of lantibiotic precursor peptide amyA6 in the process of improving the antibacterial activity of a bacillus amyloliquefaciens metabolite.
4. The use of the lantibiotic precursor peptide amyA6 according to claim 3, wherein: the addition period of the lantibiotic precursor peptide amyA6 was: the fermentation of the bacillus amyloliquefaciens starts to the end of the logarithmic phase of growth.
5. The use of the lantibiotic precursor peptide amyA6 according to claim 3, wherein: the addition amounts of the lantibiotic precursor peptide amyA6 were: 0.001-10mg/L of lanthionine precursor peptide amyA6 is added into the fermentation liquor of bacillus amyloliquefaciens.
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|---|---|---|---|---|
| WO2014202621A1 (en) * | 2013-06-20 | 2014-12-24 | Dsm Ip Assets B.V. | Carbohydrate degrading polypeptide and uses thereof |
| CN106366168A (en) * | 2016-08-26 | 2017-02-01 | 上海交通大学 | Wool sulfur peptide antimicrobial peptide and preparation method of dehydrogenation derivative of wool sulfur peptide antimicrobial peptide |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014202621A1 (en) * | 2013-06-20 | 2014-12-24 | Dsm Ip Assets B.V. | Carbohydrate degrading polypeptide and uses thereof |
| CN106366168A (en) * | 2016-08-26 | 2017-02-01 | 上海交通大学 | Wool sulfur peptide antimicrobial peptide and preparation method of dehydrogenation derivative of wool sulfur peptide antimicrobial peptide |
Non-Patent Citations (2)
| Title |
|---|
| Key Impact of an Uncommon Plasmid on Bacillus amyloliquefaciens subsp plantarum S499 Developmental Traits and Lipopeptide Production;Molinatto, G等;《FRONTIERS IN MICROBIOLOGY》;20170119;第8卷;文献号17 * |
| 解淀粉芽胞杆菌WS-8中LanM基因的克隆及生物信息学分析;崔丽婷等;《基因组学与应用生物学》;20190228;第38卷(第2期);644-649 * |
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