CN110658047A - Animal experiment pathological specimen fixing, dehydrating and decalcifying system and method - Google Patents
Animal experiment pathological specimen fixing, dehydrating and decalcifying system and method Download PDFInfo
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- CN110658047A CN110658047A CN201911101577.0A CN201911101577A CN110658047A CN 110658047 A CN110658047 A CN 110658047A CN 201911101577 A CN201911101577 A CN 201911101577A CN 110658047 A CN110658047 A CN 110658047A
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- 230000001575 pathological effect Effects 0.000 title claims abstract description 28
- 238000002474 experimental method Methods 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 title claims description 35
- 239000007788 liquid Substances 0.000 claims abstract description 237
- 238000002347 injection Methods 0.000 claims abstract description 51
- 239000007924 injection Substances 0.000 claims abstract description 51
- 238000002791 soaking Methods 0.000 claims abstract description 43
- 239000000243 solution Substances 0.000 claims description 47
- 238000007599 discharging Methods 0.000 claims description 22
- 238000007789 sealing Methods 0.000 claims description 19
- 210000001503 joint Anatomy 0.000 claims description 8
- 210000005239 tubule Anatomy 0.000 claims description 3
- 230000018044 dehydration Effects 0.000 claims description 2
- 238000006297 dehydration reaction Methods 0.000 claims description 2
- 238000009434 installation Methods 0.000 claims 1
- 230000007170 pathology Effects 0.000 claims 1
- 239000003381 stabilizer Substances 0.000 claims 1
- 210000001519 tissue Anatomy 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 230000005484 gravity Effects 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 229930040373 Paraformaldehyde Natural products 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 229920002866 paraformaldehyde Polymers 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000002360 explosive Substances 0.000 description 1
- 210000000609 ganglia Anatomy 0.000 description 1
- 239000000819 hypertonic solution Substances 0.000 description 1
- 229940021223 hypertonic solution Drugs 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000000278 spinal cord Anatomy 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/0804—Cleaning containers having tubular shape, e.g. casks, barrels, drums
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
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Abstract
The embodiment of the invention provides a fixing, dehydrating and decalcification system for pathological specimens of animal experiments, which comprises a liquid discharge assembly, a fixing assembly, a soaking assembly and a liquid injection assembly, wherein the liquid discharge assembly comprises a liquid storage tank, a liquid discharge plate, a plurality of liquid discharge funnels and a liquid discharge control mechanism, the liquid discharge control mechanism comprises a plurality of valves, valve rods, connecting rods and handles, the valves are respectively arranged at the bottoms of the liquid discharge funnels, when the soaking liquid needs to be replaced, a liquid injection disc of the liquid injection assembly is placed on the fixing plate, new soaking liquid flows into the liquid injection funnels from the liquid injection disc and then flows into specimen tubes, so that the soaking liquid in a plurality of specimen tubes is replaced at one time, the replacement speed is high, the time difference lasts for a short time before the new soaking liquid enters after the original old soaking liquid is discharged, and the time for exposing the specimens to the air is shortened, and the mode is adopted, need not remove the sample pipe with the sample, avoided the pollution that causes with the contact of outside carrier that bears.
Description
Technical Field
The invention relates to the technical field of biomedicine, in particular to a system and a method for fixing, dehydrating and decalcifying pathological specimens in animal experiments.
Background
In the later stage of the biomedical animal experiment, the obtained pathological specimen of the animal viscera needs to be fixed by a fixing solution or dehydrated in a hypertonic solution in a container, and decalcification treatment is also needed if the pathological specimen relates to a bone system so as to prepare for later-stage pathological detection. In the process, the fixing liquid, the hypertonic liquid and the decalcifying liquid need to be replaced for many times. At present, the laboratory mostly adopts an EP tube to contain a specimen, the specimen needs to be taken out after liquid is changed, the liquid is changed again after the invalid liquid is poured out, and the specimen is put into new liquid for treatment. Original structure that directly uses sample pipe to soak sample tissue has a lot of drawbacks, for example need one change when changing, changes slowly, need take out the sample tissue during the change and place on outside carrier, and this just has the pollution of carrier to the sample, also has external environment to the risk of sample pollution.
Disclosure of Invention
It is necessary to provide a system for fixing, dehydrating and decalcifying pathological specimens in animal experiments.
It also needs to provide a method for fixing, dehydrating and decalcifying pathological specimens in animal experiments.
A system for fixing, dehydrating and decalcifying pathological specimens in animal experiments comprises a liquid drainage component, a fixing component, a soaking component and a liquid injection component, wherein the liquid drainage component comprises a liquid storage tank, a liquid drainage plate, a plurality of liquid drainage funnels and a liquid drainage control mechanism, a containing cavity is arranged in the liquid storage tank, the liquid drainage plate is arranged above the liquid storage tank, a plurality of mounting holes are formed in the liquid drainage plate, the liquid drainage funnels are mounted on the mounting holes, the upper ends of the liquid drainage funnels are fixed on the liquid drainage plate, the lower ends of the liquid drainage funnels are used for discharging soaking liquid, the liquid drainage control mechanism is arranged below the liquid drainage funnels and comprises a plurality of valves, valve rods, connecting rods and handles, the valves are respectively arranged at the bottoms of the liquid drainage funnels, one ends of the valve rods are connected with the valves to control the opening and closing of the valves, the other ends of the connecting, the fixed component comprises a stand column and a fixed plate, the fixed plate is arranged above the liquid discharge plate, the stand column is arranged between the fixed plate and the liquid discharge plate, a plurality of mounting holes are formed in the fixed plate and used for mounting the soaking component, the soaking component comprises a plurality of specimen tubes, soaking liquid is contained in the specimen tubes, the specimens are soaked in the soaking liquid of the specimen tubes, the upper ends of the specimen tubes are inserted into the mounting holes of the mounting plate, the lower ends of the specimen tubes are in butt joint with the liquid discharge funnel, the liquid injection component comprises a liquid containing disc and a plurality of liquid injection funnels, supporting legs are arranged at the bottoms of the liquid containing discs and used for being placed above the fixed plate, and a plurality of mounting holes are formed in the bottoms of the liquid containing discs, the liquid injection funnels are arranged in.
Preferably, a flexible filter screen is arranged in the sample tube to prevent the sample from being discharged along with the liquid during liquid discharge.
Preferably, the sample pipe includes soaking pipe, connecting pipe, closing cap, the soaking pipe is ordinary test tube, the lower extreme of soaking pipe is the inclined plane infundibulate, and the connecting pipe is installed in the bottom of soaking pipe, sets up the external screw thread on the outer wall of connecting pipe, still sets up the internal thread on the neck inside of flowing back funnel to with the external screw thread butt joint of connecting pipe.
Preferably, annotate the height that highly is higher than the oral area of sample pipe of liquid funnel bottom, avoid annotating the bottom of liquid funnel and stretch into the sample intraduct, pollute the inside soak of sample pipe.
Preferably, the mouth of the liquid injection funnel is flush with the mounting hole at the bottom of the liquid containing tray so that the soak solution in the liquid containing tray flows into the liquid injection funnel, and the outer wall of the liquid injection funnel is in sealing connection with the mounting hole.
Preferably, the end of the valve rod is hinged with the connecting rod, so that the valve rod rotates relative to the connecting rod.
Preferably, a discharge narrow tube is further provided on the side wall of the liquid discharge tank, and a main valve is provided on the discharge narrow tube.
The method for fixing, dehydrating and decalcifying the animal experiment pathological specimen by using the system for fixing, dehydrating and decalcifying the animal experiment pathological specimen comprises the following steps:
s1, placing the specimen in a specimen tube filled with the soak solution, and keeping the required time of the test;
s2, when the soak solution needs to be replaced, the seal cover of the opening part of each specimen tube is taken down, the liquid injection assembly is arranged above the fixing assembly, and the liquid injection funnel of the liquid injection assembly is kept above the specimen tube;
s3, opening the valve of the liquid discharge control mechanism, and discharging the used invalid soak solution in all the specimen tubes at one time;
s4, closing the valve of the liquid discharge control mechanism;
s5, pouring new soak solution into the liquid injection tray, and filling each sample tube with the new soak solution;
and S6, removing the liquid injection assembly, covering the opening part of each sample pipe with a sealing cover, and keeping the required time of the experiment.
A method for fixing, dehydrating and decalcifying pathological specimens in animal experiments comprises the following steps:
s1, placing the specimen in a specimen tube filled with the soak solution, and keeping the required time of the test;
s2, when the soak solution needs to be replaced, the sealing cover of the opening part of each specimen tube is taken down, the liquid injection assembly is arranged above the fixing assembly, and the liquid injection funnel of the liquid injection assembly is kept above the specimen tube;
s3, opening the valve of the liquid discharge control mechanism, pouring new soak solution into the liquid injection tray, discharging the used soak solution in all the specimen tubes at one time, and simultaneously filling the specimen tubes with the new soak solution;
s4, closing the valve of the liquid discharge control mechanism;
and S5, removing the liquid injection assembly, covering the opening part of each sample pipe with a sealing cover, and keeping the required time of the experiment.
In the invention, because the liquid needs to be changed for many times in the pathological specimen processing process, if the whole decalcification process needs one month or even several months, the soaking liquid needs to be changed for once for several days in the decalcification period, and under the condition of large specimen quantity, the traditional method pours out the liquid one by one and then changes the liquid one by one, so the process is very time-consuming. The device only needs to open the liquid discharge control mechanism to discharge used old liquid in all the specimen tubes in batches once, and then injects new soaking liquid in batches through the liquid injection assembly to realize liquid change, thereby greatly saving the liquid change time.
In the traditional method, the tissue needs to be taken out of the liquid in the liquid changing process, and in the process, the specimen is placed in the external environment and is easily polluted by the external environment or enzymes or other factors to be detected in the tissue are damaged due to different temperatures or pH values. In the liquid changing process of the device, the sample does not need to be taken out, so that the sample is prevented from contacting with the external environment, and the problems are avoided.
In the traditional method, tissues need to be taken out of liquid in the liquid changing process, and usually, the tissues need to be clamped by ophthalmological forceps or micro forceps, and softer specimens such as brain, spinal cord and the like are easy to damage due to multiple clamping in the process. And for some small specimens, such as nerves and ganglia, the specimens are easy to lose in the process of multiple liquid changes, so that the specimen loss is caused. The flexible filter screen is arranged at the interface at the lower end of the device, so that the sample does not need to be clamped for multiple times in the liquid discharge process, the sample is prevented from being damaged, and the sample is prevented from being lost.
The traditional method needs to move a specimen tube filled with a specimen to the side of a water tank for liquid change, and the bottom of the device is provided with a liquid storage tank and a connecting pipe, so that waste liquid can be directly drained to a waste liquid cylinder or the water tank for discharge.
In the traditional method, the liquid is relatively exposed in the liquid changing process, if the fixing liquid is mostly formalin or paraformaldehyde which is inflammable, explosive, volatile and toxic and carcinogenic, the laboratory environment is polluted by the long-time exposure in the liquid changing process, and potential safety hazards are caused. The device effectively reduces the exposure time, the liquid discharge process is relatively closed, and the volatilization amount of the fixing liquid is effectively reduced, so that the problem is avoided.
In the traditional method, most of the sample tubes are directly discarded after the sample tubes are used, so that resource waste is caused, and the environment is easily polluted. If want reuse will wash the sample pipe, the cleaning process need wash once through the washing liquid, the running water washes more than 15 times, and deionized water washes more than 3 times, and can only wash single unable batch operation, and process efficiency is low, the time-consuming. This device decalcification finishes the back, takes out the sample, puts into this device with the sample pipe again, will annotate the liquid subassembly and place in the backup pad, emptys washing liquid, running water, deionized water in annotating the liquid dish in proper order, and the automatic downward flow of washing liquid is in sample pipe, flowing back funnel, realizes self-cleaning to it, keeps washing time, reaches abluent purpose many times, so can carry out batch operation, and the flowing water washes the effect and lavage the effect better for a plurality of times.
Drawings
FIG. 1 is a schematic structural diagram of a system for fixing, dehydrating and decalcifying pathological specimens in animal experiments.
Fig. 2 is a partial cross-sectional view of fig. 1.
Fig. 3 is a partially enlarged view of fig. 2.
Fig. 4 is an exploded view of fig. 1.
Fig. 5 is a cross-sectional view of a drip tray.
Fig. 6 and 7 are schematic structural views of the sample tube.
Fig. 8 is a cross-sectional view of the present device.
Fig. 9 is a partially enlarged view of fig. 8.
In the figure: the liquid storage tank 11, the discharge tubule 111, the liquid discharge plate 12, the liquid discharge funnel 13, the liquid discharge pipe 131, the valve 14, the valve rod 15, the connecting rod 16, the handle 17, the upright post 21, the fixing plate 22, the specimen pipe 31, the soaking pipe 311, the connecting pipe 312, the sealing cover 313, the flexible filter screen 314, the liquid containing disc 41 and the liquid injection funnel 42.
Detailed Description
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without creative efforts.
Referring to fig. 1 to 7, an embodiment of the present invention provides a system for fixing, dehydrating and decalcifying pathological specimens of animal experiments, including a liquid discharge assembly, a fixing assembly, a soaking assembly and a liquid injection assembly, wherein the liquid discharge assembly includes a liquid storage tank 11, a liquid discharge plate 12, a plurality of liquid discharge funnels 13 and a liquid discharge control mechanism, a containing cavity is disposed inside the liquid storage tank 11, the liquid discharge plate 12 is disposed above the liquid storage tank 11, a plurality of mounting holes are formed in the liquid discharge plate 12, the plurality of liquid discharge funnels 13 are mounted on the mounting holes, the upper ends of the liquid discharge funnels 13 are fixed on the liquid discharge plate 12, the lower ends of the liquid discharge funnels 13 are used for discharging soak solution, the liquid discharge control mechanism is disposed below the liquid discharge funnels 13, the liquid discharge control mechanism includes a plurality of valves 14, valve rods 15, connecting rods 16 and a handle 17, the plurality of valves 14 are respectively disposed at the bottoms, the other end of the connecting rod 16 is connected with one end of the connecting rod 16, the other end of the connecting rod 16 penetrates through the side wall of the liquid storage tank 11, a handle 17 is arranged at the end part of the connecting rod 16, the fixing component comprises a stand column 21 and a fixing plate 22, the fixing plate 22 is arranged above the liquid discharge plate 12, the stand column 21 is arranged between the fixing plate 22 and the liquid discharge plate 12, a plurality of mounting holes are formed in the fixing plate 22 and used for mounting a soaking component, the soaking component comprises a plurality of specimen tubes 31, soaking liquid is contained in the specimen tubes 31, specimens are soaked in the soaking liquid in the specimen tubes 31, the upper ends of the specimen tubes 31 are inserted into the mounting holes of the mounting plate, the lower ends of the specimen tubes 31 are in butt joint with the liquid discharge funnel 13, the liquid injection component comprises a liquid containing plate 41 and a plurality of liquid injection funnels 42, support legs are arranged at the bottom of the liquid containing plate 41 and used, the lower end of the liquid injection funnel 42 is placed at the mouth of the sample tube 31.
Further, still set up flexible filter screen 314 in sample pipe 31 inside to the sample is discharged along with liquid when avoiding flowing back, and can prevent that flowing back process or flowing back sample from extruding in the narrow and small space in bottom impaired.
Further, the sample tube 31 includes a soaking tube 311, a connecting tube 312 and a sealing cover 313, the soaking tube 311 is a common test tube, the lower end of the soaking tube 311 is in the shape of an inclined funnel, the connecting tube 312 is installed at the bottom of the soaking tube 311, an external thread is arranged on the outer wall of the connecting tube 312, a liquid discharge tube 131 is arranged at the bottom of the liquid discharge funnel 13, the liquid discharge tube 131 extends downwards to a length larger than that of the connecting tube, an internal thread is arranged inside the neck of the liquid discharge tube 131 to be in butt joint with the external thread of the connecting tube 312, the valve 14 is arranged on the outer wall of the liquid discharge tube 131, and the sealing cover 313 and the soaking tube 311 are detachably connected in a sealing mode. Since the dehydration and decalcification process requires 1 month or several months, the connection pipe 312 of the specimen pipe 31 is connected with the drain pipe by screw thread in the process, so that the connection is stable, and the detachable connection function is realized. The sample pipe adopts that screw connector can also conveniently take a certain sample alone and conveniently freely take off when doing experimental study to the inclined plane bottom and the 13 inclined planes of flowing back funnel butt joints of connecting pipe 312, not only the stable connection, the inclined plane also helps liquid discharge moreover, is difficult for deposit impurity or sediment.
For example, the mouth of the cover 313 and the mouth of the soaking tube 311 are screwed, and a sealing ring is provided on the inner wall of the cover 313 to achieve a sealing connection after screwing. So, when realizing whole flowing back in batches in this patent, pour into new liquid into, can also be to a sample pipe exclusive operation, for example, a certain sample pipe decalcification is accomplished, when needing to take out this solitary sample pipe, can screw up valve 14 below this sample pipe, screw up at the bar closing cap, then twist and steep pipe 311 soon, make its bottom and the separation of flowing back funnel 13, then mention this sample pipe, take out, because the closing cap sealing operation this moment, connecting pipe 312 bottom opening is also less, make the intraductal liquid of this sample keep, can not leak out from the bottom, so can take out this sample pipe alone, the purpose of independent operation has been realized, kill two birds with one stone. See fig. 8, 9. The top of the cover 313 and the side wall of the soaking tube 311 are also provided with a label which can be scrubbed for repeated use for a plurality of times, so that the information of the specimen can be conveniently marked.
Further, annotate the height that highly is higher than the oral area of sample pipe 31 of liquid funnel 42 bottom, avoid annotating the bottom of liquid funnel 42 and stretch into inside the sample pipe 31, pollute the inside soak of sample pipe 31.
Furthermore, the mouth of the liquid injection funnel 42 is flush with the mounting hole at the bottom of the liquid containing tray 41, so that the soak solution in the liquid containing tray 41 flows into the liquid injection funnel 42, and the outer wall of the liquid injection funnel 42 is hermetically connected with the mounting hole. When changing the soak solution, the notes liquid dish that will annotate the liquid subassembly is placed on fixed plate 22, again with the leading-in notes liquid dish of new soak solution, flow into by annotating the liquid dish and annotate in liquid funnel 42, and then flow into in the sample pipe 31, the soak solution in the many sample pipes 31 of once only having so realized changing, and it is fast to change, also make original old soak solution discharge back, before new soak solution gets into, the time that this time difference lasts is shorter, thereby make the time that the sample exposes in the air shorten, and this mode, need not remove sample pipe 31 with the sample, the pollution that causes with the contact of outside carrier has been avoided.
Further, the end of the valve rod 15 is hinged to the connecting rod 16, so that the valve rod 15 rotates relative to the connecting rod 16. When liquid needs to be discharged, the handle is pulled, the connecting rod 16 moves to one side, and the valve rod 15 is driven to rotate 90 degrees, so that the valve 14 is opened, and liquid is discharged.
Further, a narrow discharge pipe 111 is provided on the side wall of the liquid discharge tank, and a main valve is provided on the narrow discharge pipe 111.
The invention also provides a method for fixing, dehydrating and decalcifying the pathological specimen of the animal experiment by using the system for fixing, dehydrating and decalcifying the pathological specimen of the animal experiment, which comprises the following steps:
s1, placing the specimen in the specimen tube 31 filled with the soak solution, and keeping the required time of the test;
s2, when the soak solution needs to be replaced every three days, the sealing cover 313 of the opening part of each sample tube 31 is taken down, the liquid injection assembly is arranged above the fixing assembly, and the liquid injection funnel 42 of the liquid injection assembly is kept above the sample tube 31;
s3, opening the valve 14 of the liquid discharge control mechanism, and discharging all the used soaking liquid in the sample tube 31 at one time;
s4, closing the valve 14 of the liquid discharge control mechanism;
s5, pouring new soaking liquid into the liquid injection tray, and filling each sample tube 31 with the new soaking liquid;
and S6, removing the liquid injection assembly, covering the opening part of each sample tube 31 with a sealing cover 313, and keeping the required time of the experiment.
The invention also provides a method for fixing, dehydrating and decalcifying the pathological specimen of the animal experiment, which comprises the following steps:
s1, placing the specimen in the specimen tube 31 filled with the soak solution, and keeping the required time of the test;
s2, when the soak solution needs to be replaced every three days, the sealing cover 313 of the opening part of each sample tube 31 is taken down, the liquid injection assembly is arranged above the fixing assembly, and the liquid injection funnel 42 of the liquid injection assembly is kept above the sample tube 31;
s3, opening the valve 14 of the liquid discharge control mechanism, pouring new soaking liquid into the liquid injection tray, discharging all used soaking liquid in the sample tube 31 at one time, and simultaneously filling the sample tube 31 with the new soaking liquid;
s4, closing the valve 14 of the liquid discharge control mechanism;
and S5, removing the liquid injection assembly, covering the opening part of each sample tube 31 with a sealing cover 313, and keeping the required time of the experiment.
In the scheme, the soak solution discharged from the bottom and the injected new soak solution are synchronously carried out, so that the risk that the soak solution is completely discharged when the separate operation is carried out is avoided, and when the new soak solution is not injected, the specimen is exposed in the air and is polluted by impurities in the air. In this scheme, new soak and old soak seamless butt joint for the sample is in the soak all the time, does not have the problem with the air contact.
Because the specific gravity of the soak solution is less than the density of the specimen, the specimen sinks to the bottom of the specimen tube 31 all the time, and because the old soak solution slightly volatilizes after being used for three days, the specific gravity of the old soak solution is slightly larger than that of the new soak solution, so that the new soak solution is added while the old soak solution is discharged, the old soak solution is actively discharged downwards due to the slightly heavier specific gravity, and the problem that the old soak solution cannot be completely discharged does not exist.
For example, in the present invention, the soaking solution is formalin or paraformaldehyde.
The modules or units in the device of the embodiment of the invention can be combined, divided and deleted according to actual needs.
The foregoing is directed to embodiments of the present invention, which do not limit the scope of the invention, and the invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. It will be understood by those skilled in the art that all or a portion of the above-described embodiments may be practiced and equivalents thereof may be resorted to as falling within the scope of the invention as claimed. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may include only a single embodiment, and such description is for clarity only, and those skilled in the art will be able to make the description as a whole, and the embodiments may be suitably combined to form other embodiments as will be apparent to those skilled in the art.
Claims (9)
1. The utility model provides an animal experiment pathology sample is fixed, dehydration, decalcification system which characterized in that: the liquid discharging component comprises a liquid storage tank, a liquid discharging plate, a plurality of liquid discharging funnels and a liquid discharging control mechanism, wherein a containing cavity is arranged in the liquid storage tank, the liquid discharging plate is arranged above the liquid storage tank, a plurality of mounting holes are formed in the liquid discharging plate, the liquid discharging funnels are mounted on the mounting holes, the upper ends of the liquid discharging funnels are fixed on the liquid discharging plate, the lower ends of the liquid discharging funnels are used for discharging soaking liquid, the liquid discharging control mechanism is arranged below the liquid discharging funnels, the liquid discharging control mechanism comprises a plurality of valves, valve rods, connecting rods and handles, the valves are respectively arranged at the bottoms of the liquid discharging funnels, one ends of the valve rods are connected with the valves to control the valves to be opened and closed, the other ends of the valve rods are connected with one ends of the connecting rods, the other ends of the connecting rods penetrate through the side walls, the fixed plate sets up in the drain bar top, and the stand sets up between fixed plate and drain bar, offers a plurality of mounting holes on the fixed plate for the installation soaks the subassembly, it includes a plurality of sample pipes to soak the subassembly, and the sample is inside to hold the soak, and the sample soaks in the soak of sample pipe, and inside the mounting hole of mounting panel was inserted to the upper end of sample pipe, lower extreme and flowing back funnel butt joint, annotate the subassembly and include flourishing liquid dish, a plurality of notes liquid funnel, the bottom of flourishing liquid dish sets up the stabilizer blade for place in the top of fixed plate, still set up a plurality of mounting holes at the end of the dish of flourishing liquid dish, annotate the liquid funnel set up in the mounting hole, the lower extreme of annotating the.
2. The system for fixing, dehydrating and decalcifying an animal experimental pathological specimen as set forth in claim 1, wherein: still set up flexible filter screen at the sample intraduct to the sample is discharged along with liquid when avoiding the flowing back.
3. The system for fixing, dehydrating and decalcifying an animal experimental pathological specimen as set forth in claim 1, wherein: the sample tube comprises a soaking tube, a connecting tube and a sealing cover, the soaking tube is a common test tube, the lower end of the soaking tube is in an inclined plane funnel shape, the connecting tube is installed at the bottom of the soaking tube, external threads are arranged on the outer wall of the connecting tube, and internal threads are further arranged inside the neck of the liquid discharge funnel to be in butt joint with the external threads of the connecting tube.
4. The system for fixing, dehydrating and decalcifying an animal experimental pathological specimen as set forth in claim 1, wherein: annotate the height that highly is higher than the oral area of sample pipe of liquid funnel bottom, avoid annotating the bottom of liquid funnel and stretch into the sample intraduct, pollute the inside soak of sample pipe.
5. The system for fixing, dehydrating and decalcifying an animal experimental pathological specimen as set forth in claim 1, wherein: the oral area of notes liquid funnel and the mounting hole parallel and level of flourishing liquid dish end to in making the soak in the flourishing liquid dish flow into notes liquid funnel, sealing connection between the outer wall of notes liquid funnel and the mounting hole.
6. The system for fixing, dehydrating and decalcifying an animal experimental pathological specimen as set forth in claim 1, wherein: the end part of the valve rod is hinged with the connecting rod, so that the valve rod rotates relative to the connecting rod.
7. The system for fixing, dehydrating and decalcifying an animal experimental pathological specimen as set forth in claim 1, wherein: and a discharge tubule is arranged on the side wall of the liquid discharge groove, and a main valve is arranged on the discharge tubule.
8. A method for fixing, dehydrating and decalcifying an animal experimental pathological specimen by using the system for fixing, dehydrating and decalcifying an animal experimental pathological specimen according to any one of claims 1 to 7, comprising the steps of:
s1, placing the specimen in a specimen tube filled with the soak solution, and keeping the required time of the test;
s2, when the soak solution needs to be replaced, the seal cover of the opening part of each specimen tube is taken down, the liquid injection assembly is arranged above the fixing assembly, and the liquid injection funnel of the liquid injection assembly is kept above the specimen tube;
s3, opening the valve of the liquid discharge control mechanism to discharge the used soak solution in all the specimen tubes at one time;
s4, closing the valve of the liquid discharge control mechanism;
s5, pouring new soak solution into the liquid injection tray, and filling each sample tube with the new soak solution;
and S6, removing the liquid injection assembly, covering the opening part of each sample pipe with a sealing cover, and keeping the required time of the experiment.
9. A method for fixing, dehydrating and decalcifying animal experiment pathological specimens is characterized by comprising the following steps:
s1, placing the specimen in a specimen tube filled with the soak solution, and keeping the required time of the test;
s2, when the soak solution needs to be replaced, the seal cover of the opening part of each specimen tube is taken down, the liquid injection assembly is arranged above the fixing assembly, and the liquid injection funnel of the liquid injection assembly is kept above the specimen tube;
s3, opening the valve of the liquid discharge control mechanism, pouring new soak solution into the liquid injection tray, discharging the used soak solution in all the specimen tubes at one time, and simultaneously filling the specimen tubes with the new soak solution;
s4, closing the valve of the liquid discharge control mechanism;
and S5, removing the liquid injection assembly, covering the opening part of each sample pipe with a sealing cover, and keeping the required time of the experiment.
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