CN110646410A - Cardiovascular and cerebrovascular disease multi-index joint diagnosis method based on combination of portable scanner and microfluidic filter paper chip - Google Patents
Cardiovascular and cerebrovascular disease multi-index joint diagnosis method based on combination of portable scanner and microfluidic filter paper chip Download PDFInfo
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Abstract
The invention discloses a cardiovascular and cerebrovascular disease multi-index joint diagnosis method based on a portable scanner combined with a microfluidic filter paper chip, which is characterized in that the concentration of blood uric acid, blood sugar and triglyceride is calculated simultaneously, the measured concentration value is compared with the concentration value in a normal human body, and then whether a detection object has the risk of cardiovascular and cerebrovascular diseases is judged jointly by comparing the difference value; the concentrations of the blood uric acid, the blood sugar and the triglyceride are obtained by joint detection of a micro-control filter paper chip, a scanner, an SD memory card with a Wi-Fi module and a smart phone. The method is based on the joint analysis of three indexes closely related to cardiovascular and cerebrovascular diseases, and simultaneously uses a widely used portable scanner and a smartphone which respectively have accurate image restoration capability and image analysis function, so that the method has the obvious advantages of high diagnosis accuracy, high detection speed, strong portability, high detection precision and easiness in popularization.
Description
Technical Field
The invention belongs to the field of medicine, and particularly relates to a cardiovascular and cerebrovascular disease multi-index joint diagnosis method based on combination of a portable scanner and a microfluidic filter paper chip, which is used for detecting respective concentrations of blood uric acid, blood sugar and triglyceride of a detected object by combining the microfluidic filter paper chip with the portable scanner and a smart phone, and comparing the concentrations with normal human body concentrations to realize the purpose of cardiovascular and cerebrovascular disease diagnosis.
Background
The prevalence rate of cardiovascular diseases in China is in a continuously rising stage. One of the reasons for the high fatality rate of cardiovascular and cerebrovascular diseases is that patients cannot accurately diagnose the diseases in the early stage of the diseases, and especially in rural areas, the relatively laggard medical level increases the difficulty of early diagnosis of the cardiovascular and cerebrovascular diseases.
The prior publication numbers are: 201810655810.9 discloses a cardiovascular and cerebrovascular disease detection device, which provides ECG, blood pressure, blood oxygen, pulse, heart sound, body temperature, photoelectric volume signal detection and processing, extracts characteristic value, classifies the characteristic value signal, and determines cardiovascular and cerebrovascular health status according to classification result and cardiovascular and cerebrovascular disease prediction model. However, the method is complex in flow and high in cost of the detection device, and is not easy to popularize in poor backward areas.
The prior publication numbers are: CN 108648827 a discloses a method and a device for predicting cardiovascular and cerebrovascular disease risk, but this method has low detection accuracy, and only by comparison, the actual detection object is not accurately analyzed, so the accuracy is not high;
at present, the accurate diagnosis method for cardiovascular and cerebrovascular diseases usually depends on large-scale instruments, complex operation and professional technical ability, so that the accurate and rapid diagnosis for cardiovascular and cerebrovascular diseases still cannot be popularized in laggard rural areas.
The scanner has many advantages such as eliminating the influence of the ambient light intensity on the imaging and having a light source close to the sunlight, and is receiving attention, especially in the colorimetric analysis, because it can obtain high quality sample pictures through the scanner, it is a popular choice for many researchers. Quantitative detection of human IgG was achieved using transmission scan imaging as described by Wen et al (Jing Wen, Xiaooli Shi., YiningHe., Jianjun Zhou, Yunchao Li., Novel plastics biochips for colorimetric detection of biomolecules, Analytical and biochemical chemistry, 2012, 404 (6-7): 1935-. In addition, Abe et al prepared Microfluidic channels on filter paper using Inkjet printing technology and imaged with a scanner to achieve organophosphorus pesticide residue detection (Abe K, Suzuki K, CitterioD, Inkjet-Printed microfluidics multiorganic Chemical Sensing paper 2008, 80(18): 6928-. Soldat et al further demonstrated that colorimetric analysis could, to some extent, completely replace the spectrometer by scanning the solution in a 96 micro cuvette. (Douglas J.Soldat, Phillip Barak, and Brian J.Lepore, Microscale colorimetric genomic Using a Desktop Scanner and Automated Digital Image analysis. journal Chemical discovery, 2009,86(5): 617-. The research results make people want to combine the scanner and the accurate detection more. However, the following problems are present. Firstly, most of scanners used for quantitative detection are office desktop scanners which have large volume and low integration degree and are not beneficial to the portable movement of equipment; secondly, the dependence of the image acquisition and analysis process of the current scanner on a computer is too strong, and the scanner mostly needs an external power supply and other ports, so that the field rapid quantitative detection of partial areas is difficult to complete; thirdly, the image analysis and calculation process is complex, most of the image analysis and calculation processes can be completed only by manual calculation of a detector, so that the operation difficulty is increased, and the probability of generating errors is increased. Further improvements are therefore desirable.
The prior publication numbers are: CN 108489973A discloses a device and a method for realizing quantitative detection of concentration based on an integrated scanner, which develops the integrated scanner and realizes the image processing function by using STM32 development board to replace a desktop computer. However, the new integrated device has certain requirements for the operation of users, so that the popularization of the new integrated device in villages and towns and partial remote areas can be difficult.
With the continuous development and innovation of the microfluidic technology, the microfluidic paper chip is also gradually paid more attention by researchers as a branch stream. Two-dimensional microfluidic paper chips prepared by a photoresist method are bonded together by double-sided adhesive as early as 2008, and then packaged to obtain a three-dimensional microfluidic paper core (A.W. Martinez, S.T.Phillips, G.M. whites, Proceedings of the National Academy of Sciences,105 (2008) 19606-. Subsequently in 2010, they again carried out the preparation of editable three-dimensional microfluidic paper Chip diagnostic devices (a.w. Martinez, s.t. Phillips, z. Nie, c.m. Cheng, e. Carrilho, b.j. Wiley, g.m. Whitesides, Lab on a Chip, 10 (2010) 2499-.
Publication No. CN 105107557A discloses "a preparation method and application of a high-throughput three-dimensional microfluidic paper chip", and proposes that a hydrophilic channel and a hydrophobic substrate are adhered together to form the three-dimensional microfluidic paper chip. Although the method can be used for high-throughput detection, the obtained paper chip can cause pollution because the reaction liquid is attached to a scanner, so that the method cannot be directly used for scanning and imaging of the scanner. Therefore, a microfluidic paper chip which can be directly used for scanning analysis of a scanner is still lacking at present.
Disclosure of Invention
The invention aims to solve the specific technical problems that the existing cardiovascular and cerebrovascular disease diagnosis technology can be realized only by carrying out quantitative detection on a certain index of a human body through an expensive large-scale instrument and complicated operation, and other analysis methods proposed by people before simplify the detection process, but have the problems of low detection precision, poor portability and the like, and the judgment on the cardiovascular and cerebrovascular diseases is finished only by one index often has errors, thus being not beneficial to the rapid and accurate disease diagnosis on site. Therefore, the invention provides a multi-index combined diagnosis method for cardiovascular and cerebrovascular diseases based on a portable scanner and a microfluidic filter paper chip, and aims to drop the uric acid, blood sugar and triglyceride in human blood on the manufactured microfluidic filter paper chip after different color reactions are completed, then use the portable scanner to scan the filter paper chip, transmit an electronic picture to a smart phone for analysis and calculation through a Wi-Fi module carried by an SD memory card, and perform combined comparison analysis on the concentrations of the three indexes and the normal human body to perform early diagnosis on the cardiovascular and cerebrovascular diseases.
The invention adopts the following technical scheme:
a multi-index combined diagnosis method for cardiovascular and cerebrovascular diseases based on a portable scanner combined with a microfluidic filter paper chip is characterized in that the concentrations of blood uric acid, blood sugar and triglyceride are calculated simultaneously, the measured concentration values are compared with the concentration values in normal human bodies, and then whether a detected object has the risk of the cardiovascular and cerebrovascular diseases is judged jointly by comparing the difference values.
The concentration of the blood uric acid, the blood sugar and the triglyceride is obtained by joint detection of a micro-control filter paper chip, a scanner, an SD storage card with a Wi-Fi module and a smart phone, the micro-control filter paper chip reacts with a sample of the blood uric acid, the blood sugar and the triglyceride for color development, the scanner scans the micro-control filter paper chip after color development to obtain a digital image and stores the digital image in the SD storage card, and the digital image is transmitted to the smart phone through the Wi-Fi module and stored; and then, calculating the colorimetric values and concentration values of the developed samples to obtain specific concentration values of blood uric acid, blood sugar and triglyceride.
The micro-control filter paper chip comprises three areas for detecting blood uric acid, blood sugar and triglyceride, wherein each area is divided into a standard sample area and a sample area to be detected, the standard sample area is used for establishing a fitting equation during data analysis, and the sample area to be detected is an actual sample reaction area to be detected.
The micro-control filter paper chip is formed by bonding four layers, the ground layer is a substrate, the middle layer is a detection layer and comprises a hydrophobic area and a hydrophilic area, and the top two layers are isolation layers; the specific manufacturing process is as follows:
1) designing a substrate configuration, a detection layer hydrophilic area and hydrophobic area configuration and an isolation layer configuration, and printing on filter paper;
2) shearing the substrate configuration printed on the filter paper, the hydrophilic area and hydrophobic area configuration of the detection layer and the isolation layer configuration, and respectively obtaining the substrate configuration, the hydrophobic area and hydrophilic area of the detection layer and the isolation layer configuration;
3) methyltrichlorosilane and octadecyltrichloroalkane were reacted at room temperature in a 1: dissolving the mixture in a normal hexane solution according to the volume ratio of 10, and uniformly mixing;
4) placing the substrate layer sheared in the step (2), the hydrophobic area of the detection layer and the isolation layer in the mixed solution in the step (3) at room temperature, and soaking for 5 minutes;
5) taking out the substrate layer, the hydrophobic area of the detection layer and the isolation layer soaked in the step (4), and naturally drying for 15 minutes;
and (3) placing the hydrophilic area of the detection layer cut in the step (2) in the detection layer obtained in the step (5) to obtain the four-layer filter paper chip. The four-layer filter paper chip is adhered by daily-used fog-type spray glue.
The hole pattern of the hydrophilic region of the detection layer is one of a circular pattern, a square pattern and a triangular pattern. The size of the hydrophilic area of the detection layer can be adjusted, and the number of the circular detection areas can also be changed.
The diameter or side length of the holes of the hydrophilic area of the detection layer is not less than 6 mm.
Compared with the prior detection technology, the invention has the following advantages: (1) compared with other colorimetric analysis methods, the method has the advantages of high detection precision, high detection speed, simplicity in operation and the like; (2) the self-made microfluidic filter paper chip has the advantages of multi-flux detection, low cost and the like; (3) the combination of the portable scanner and the smart phone not only keeps the advantage of high-quality image restoration of the scanner, but also utilizes the strong data analysis and processing capacity of the smart phone, and the device has small occupied volume and is easy to carry and detect on site; (4) the portable scanner and the smart phone are popularized in the market, and the software for importing and analyzing the images can be downloaded and installed from the internet; (5) the method for diagnosing cardiovascular and cerebrovascular diseases by combining multiple indexes improves the accuracy of diagnosis.
Drawings
FIG. 1 is a schematic diagram of a detection apparatus employed in the present invention;
FIGS. 2 and 3 are schematic structural views of a micro-control filter paper chip;
FIG. 4 is a diagram showing the actual scanning effect of the micro-control filter paper chip;
FIG. 5 is a standard curve graph of R value as a function of uric acid concentration.
Detailed Description
The method of the invention calculates the concentrations of blood uric acid, blood sugar and triglyceride at the same time, compares the measured concentration value with the concentration value in a normal human body, and then judges whether the detected object has the risk of cardiovascular and cerebrovascular diseases or not by comparing the difference value.
The adopted equipment comprises a micro-control filter paper chip, a scanner, an SD memory card with a Wi-Fi module and a smart phone. The micro-control filter paper chip is divided into three parts of a uric acid area, a glucose area and a triglyceride area, and each detection area can be divided into a standard sample area and a sample area to be detected. The standard sample area is used for establishing a fitting equation according to the concentration value and the chromatic value of the known sample and calculating the concentration of the sample to be detected. The microfluidic chip is formed by cutting four layers of filter paper and then pasting the cut filter paper with glue, and the bottom layer is a substrate; the detection layer is used for carrying out color reaction and comprises a hydrophobic area and a hydrophilic area; the two isolating layers are used for avoiding pollution caused by contact of a sample and a scanner in the scanning process. The specific manufacturing steps of the microfluidic filter paper chip are as follows. Firstly, cutting Whatman Grade 3 qualitative filter paper; then, carrying out hydrophobization treatment on the parts except the detection area, specifically soaking filter paper in a mixed solution of MTS (OTS =10: 1) for 10min, and then naturally air-drying; and finally, sticking the four layers of filter paper together through the daily used fog-type spray glue.
And respectively and sequentially dripping the standard sample and the sample to be detected after the reaction and the color development in a reaction area on the third layer of filter paper, and scanning the filter paper chip by using a portable scanner to obtain a digital image. And then, the image in the scanner is transmitted to the smart phone through a Wi-Fi module carried by the SD memory card and is stored. And then, loading and analyzing pictures by using mobile phone software which is independently developed in a mobile phone, so as to realize the establishment of a fitting equation and the quantitative detection of the sample to be detected. The fitting equation of the chromatic value and the concentration is that the chromatic value of a standard sample with known concentration is calculated firstly through the smart phone, and the fitting equation of the chromatic value and the concentration is established by utilizing the concentration value and the chromatic value. Reference is made to prior application 201810187863.2 for the specific steps involved in establishing the fitting equation and detecting the concentration.
The specific embodiment is as follows:
(1) making a filter paper substrate: and cutting the filter paper according to the designed size to obtain four layers of filter paper and 52 small reaction area discs. The four layers were then hydrophobized by soaking them in a mixture of OTS (octadecyltrifluorosilane) and MTS (methyltrifluorosilane) at a ratio of 1: soaking in hydrophobic solvent prepared at ratio of 10 for 5 min. And after drying, sticking the filter paper subjected to the hydrophobic treatment and the small round piece together by using glue spraying.
(2) Preparation and color reaction of uric acid standard sample: 1g/L of uric acid standard solution is diluted into standard samples with the concentrations of 1, 2, 4, 6, 8 and 10mg/dL respectively. Taking uric acid with different concentrations and blank samples of 200 mu L respectively, adding tungstic acid protein precipitant of 1000 mu L, mixing uniformly, standing for 10min, centrifuging in a centrifuge for 5min at 3000r/min, taking supernatant of 160 mu L, adding CUT reagent of 50 mu L, adding phosphotungstic acid reagent of 50 mu L, and mixing uniformly to obtain color gradient.
(3) Preparation and color reaction of glucose standard sample: 1mol/L glucose standard solution is diluted into standard samples with the concentrations of 1, 2, 4, 6, 8 and 10mmol/dL respectively. 10 mu L of glucose with different concentrations and blank samples are respectively taken, 1000 mu L of color developing agent is respectively added into the samples, and color gradient is presented after uniform mixing.
(4) Preparation and color reaction of triglyceride standard sample: the triglyceride standard solution of 1g/L is diluted into standard solutions with the concentrations of 20, 40, 100, 150, 180 and 200mg/dL respectively. 10 mu L of each triglyceride and blank sample with different concentrations are taken, 1000 mu L of color developing agent is respectively added into the triglyceride and blank samples, and color gradient is presented after uniform mixing.
(5) And respectively dripping the reaction liquids which are arranged according to concentration gradient after color development on a filter paper chip to serve as standard samples, respectively dripping uric acid reaction liquids with the concentrations of 4mg/dL and 8mg/dL, glucose reaction liquids with the concentrations of 4mmol/dL and 8mmol/dL and triglyceride reaction liquids with the concentrations of 100mg/dL and 180mg/dL to serve as samples to be detected on sample areas to be detected, and dripping 30 mu L of all the samples. The filter paper chip was then scanned using a portable scanner. And after the scanning is finished, the pictures are transmitted to the smart phone by utilizing the Wi-Fi module of the SD memory card. And then, software is started in the smart phone, and a picture obtained by the smart phone from the handheld scanner is selected to complete the establishment of the fitting equation and the detection of the sample to be detected. Finally, the detected concentrations of the two uric acid samples to be detected are respectively 3.92mg/dL and 8.00 mg/dL; the detected concentrations of the two glucose samples to be detected are respectively 3.90mmol/L and 8.08 mmol/L; the results of the detection concentrations of the two triglyceride samples to be detected of 99.24mg/dL and 181.57mg/dL. respectively meet the error requirement.
(6) Colorimetric analysis preparation of standard curve: after the images transmitted by the portable scanner are opened by the mobile phone image analysis software, the colorimetric values R of the detection areas under different uric acid concentrations are respectively read. The results show that: the change in R-value decreased with increasing uric acid concentration. Thus, a standard graph is prepared, as shown in FIG. 5.
The following are experimental examples based on the diagnostic method of the present invention:
experimental example 1
The method for detecting the actual sample obtained from the large hospital in Shanxi province comprises the following steps:
(1) making a filter paper substrate: and cutting the filter paper according to the designed size to obtain four layers of filter paper and 52 small reaction area discs. The four layers were then hydrophobized by soaking them in a mixture of OTS (octadecyltrifluorosilane) and MTS (methyltrifluorosilane) at a ratio of 1: soaking in hydrophobic solvent prepared at ratio of 10 for 5 min. And after drying, sticking the filter paper subjected to the hydrophobic treatment and the small round piece together by using glue spraying.
(2) 1mL of serum is averagely divided into three parts which are respectively used for detecting uric acid, glucose and triglyceride;
(3) preparation of uric acid standard sample and treatment of actual sample: 1g/L of uric acid standard solution is diluted into standard samples with the concentrations of 1, 2, 4, 6, 8 and 10mg/dL respectively. Taking blank samples, uric acid with different concentrations and actual sample serum 200 muL respectively, adding tungstic acid protein precipitant 1000 muL, mixing uniformly, standing for 10min, centrifuging in a centrifuge for 5min at 3000r/min, taking supernatant 160 muL after centrifugation, adding CUT reagent 50 muL, adding phosphotungstic acid reagent 50 muL, mixing uniformly, and developing the color of the standard sample by color gradient, wherein the actual sample is developed with color reaction to develop the color.
(4) Preparation of glucose standard sample and treatment of actual sample: 1mol/L glucose standard solution is diluted into standard samples with the concentrations of 1, 2, 4, 6, 8 and 10mmol/dL respectively. Taking blank samples, glucose with different concentrations and serum of actual samples to be 10 mu L respectively, adding 1000 mu L of color developing agent into the blank samples, mixing the blank samples, the glucose with different concentrations and the serum of the actual samples uniformly, and then developing the color of the standard samples by color developing reaction.
(5) Preparation of triglyceride standards and treatment of actual samples: the triglyceride standard solution of 1g/L is diluted into standard solutions with the concentrations of 20, 40, 100, 150, 180 and 200mg/dL respectively. Taking blank samples, triglyceride with different concentrations and serum of actual samples to be 10 mul respectively, adding 1000 mul of color developing agent into the blank samples, mixing the blank samples, the triglyceride with different concentrations and the serum of the actual samples, and then developing the color of the standard samples by color developing reaction.
(6) And respectively dripping each standard sample which is arranged according to concentration gradient after color development into a color development area on the filter paper chip, respectively dripping the actual samples of uric acid, glucose and triglyceride after color development into a sample area to be detected, and dripping 30 mu L of all the standard samples and the samples to be detected. The filter paper chip was then scanned using a portable scanner. And after the scanning is finished, the pictures are transmitted to the smart phone by utilizing the Wi-Fi module of the SD memory card. And then, software is started in the smart phone, and a picture obtained by the smart phone from the handheld scanner is selected to complete the establishment of the fitting equation and the detection of the actual sample. The actual concentration of uric acid sample is 4.92mg/dL, the actual concentration of glucose sample is 5.33mg/dL, and the actual concentration of triglyceride sample is 107.61 mg/dL. The concentrations of the three indexes of the actual sample are all in a normal range, so that the probability that the detection object of the sample is suffered from the cardiovascular disease is very small.
(7) The test results of the actual samples obtained from the hospital were: uric acid 4.68mg/dL, glucose 5.7mg/dL, triglyceride 109.49mg/dL, and hospital-to-patient diagnostic results: normal index and less risk of cardiovascular diseases. Consistent with the diagnostic results of the present invention.
Experimental example 2
The method for detecting the actual sample obtained from the large hospital in Shanxi province comprises the following steps:
(1) making a filter paper substrate: and cutting the filter paper according to the designed size to obtain four layers of filter paper and 52 small reaction area discs. The four layers were then hydrophobized by soaking them in a mixture of OTS (octadecyltrifluorosilane) and MTS (methyltrifluorosilane) at a ratio of 1: soaking in hydrophobic solvent prepared at ratio of 10 for 5 min. And after drying, sticking the filter paper subjected to the hydrophobic treatment and the small round piece together by using glue spraying.
(2) 1mL of serum is averagely divided into three parts which are respectively used for detecting uric acid, glucose and triglyceride;
(3) preparation of uric acid standard sample and treatment of actual sample: 1g/L of uric acid standard solution is diluted into standard samples with the concentrations of 1, 2, 4, 6, 8 and 10mg/dL respectively. Taking blank samples, uric acid with different concentrations and actual sample serum 200 muL respectively, adding tungstic acid protein precipitant 1000 muL, mixing uniformly, standing for 10min, centrifuging in a centrifuge for 5min at 3000r/min, taking supernatant 160 muL after centrifugation, adding CUT reagent 50 muL, adding phosphotungstic acid reagent 50 muL, mixing uniformly, and developing the color of the standard sample by color gradient, wherein the actual sample is developed with color reaction to develop the color.
(4) Preparation of glucose standard sample and treatment of actual sample: 1mol/L glucose standard solution is diluted into standard samples with the concentrations of 1, 2, 4, 6, 8 and 10mmol/dL respectively. Taking blank samples, glucose with different concentrations and serum of actual samples to be 10 mu L respectively, adding 1000 mu L of color developing agent into the blank samples, mixing the blank samples, the glucose with different concentrations and the serum of the actual samples uniformly, and then developing the color of the standard samples by color developing reaction.
(5) Preparation of triglyceride standards and treatment of actual samples: the triglyceride standard solution of 1g/L is diluted into standard solutions with the concentrations of 20, 40, 100, 150, 180 and 200mg/dL respectively. Taking blank samples, triglyceride with different concentrations and serum of actual samples to be 10 mul respectively, adding 1000 mul of color developing agent into the blank samples, mixing the blank samples, the triglyceride with different concentrations and the serum of the actual samples, and then developing the color of the standard samples by color developing reaction.
(6) And respectively dripping each standard sample which is arranged according to concentration gradient after color development into a color development area on the filter paper chip, respectively dripping the actual samples of uric acid, glucose and triglyceride after color development into a sample area to be detected, and dripping 30 mu L of all the standard samples and the samples to be detected. The filter paper chip was then scanned using a portable scanner. And after the scanning is finished, the pictures are transmitted to the smart phone by utilizing the Wi-Fi module of the SD memory card. And then, software is started in the smart phone, and a picture obtained by the smart phone from the handheld scanner is selected to complete the establishment of the fitting equation and the detection of the actual sample. The actual concentration of uric acid sample is 7.23mg/dL, the actual concentration of glucose sample is 8.47mg/dL, and the actual concentration of triglyceride sample is 146.21 mg/dL. In the actual sample detection result, uric acid and glucose are higher than those of a normal human body, and triglyceride is in the normal range but is higher, so that the detection object of the sample can be judged to have relatively higher probability of suffering from cardiovascular diseases.
(7) The test results of the actual samples obtained from the hospital were: uric acid 7.35mg/dL, glucose 8.20mg/dL, triglyceride 144.72mg/dL, and hospital-to-patient diagnostic results: has higher probability of cardiovascular diseases. Consistent with the diagnostic results of the present invention.
Claims (6)
1. A cardiovascular and cerebrovascular disease multi-index combined diagnosis method based on a portable scanner combined with a microfluidic filter paper chip is characterized in that the concentration of blood uric acid, blood sugar and triglyceride is calculated simultaneously, the measured concentration value is compared with the concentration value in a normal human body, and then whether a detection object has the risk of cardiovascular and cerebrovascular diseases is judged jointly by comparing the difference value.
2. The cardiovascular and cerebrovascular disease multi-index joint diagnosis method based on the combination of the portable scanner and the microfluidic filter paper chip as claimed in claim 1, wherein the concentrations of blood uric acid, blood glucose and triglyceride are obtained by joint detection of the micro-control filter paper chip, the scanner, the SD storage card with the Wi-Fi module and the smart phone, the micro-control filter paper chip reacts with the blood uric acid, blood glucose and triglyceride samples for color development, the scanner scans the micro-control filter paper chip after color development to obtain digital images and stores the digital images in the SD storage card, and the digital images are transmitted to the smart phone through the Wi-Fi module and stored; and then, calculating the colorimetric values and concentration values of the developed samples to obtain specific concentration values of blood uric acid, blood sugar and triglyceride.
3. The cardiovascular and cerebrovascular disease multi-index joint diagnosis method based on portable scanner combined with microfluidic filter paper chip as claimed in claim 2, wherein the micro-controlled filter paper chip comprises three regions for detecting blood uric acid, blood sugar and triglyceride, each region is divided into a standard sample region and a "sample region to be tested", wherein the "standard sample region" is used for establishing a fitting equation during data analysis, and the "sample region to be tested" is an actual sample reaction region to be tested.
4. The cardiovascular and cerebrovascular disease multi-index joint diagnosis method based on portable scanner combined microfluidic filter paper chip as claimed in claim 2 or 3, wherein the micro-controlled filter paper chip is formed by four layers of bonding, the ground layer is a substrate, the middle layer is a detection layer comprising a hydrophobic region and a hydrophilic region, and the top two layers are isolation layers; the specific manufacturing process is as follows:
designing a substrate configuration, a detection layer hydrophilic area and hydrophobic area configuration and an isolation layer configuration, and printing on filter paper;
shearing the substrate configuration printed on the filter paper, the hydrophilic area and hydrophobic area configuration of the detection layer and the isolation layer configuration, and respectively obtaining the substrate configuration, the hydrophobic area and hydrophilic area of the detection layer and the isolation layer configuration;
methyltrichlorosilane and octadecyltrichloroalkane were reacted at room temperature in a 1: dissolving the mixture in a normal hexane solution according to the volume ratio of 10, and uniformly mixing;
placing the substrate layer sheared in the step (2), the hydrophobic area of the detection layer and the isolation layer in the mixed solution in the step (3) at room temperature, and soaking for 5 minutes;
taking out the substrate layer, the hydrophobic area of the detection layer and the isolation layer soaked in the step (4), and naturally drying for 15 minutes;
and (3) placing the hydrophilic area of the detection layer cut in the step (2) in the detection layer obtained in the step (5) to obtain the four-layer filter paper chip.
5. The cardiovascular and cerebrovascular disease multi-index joint diagnosis method based on portable scanner combined with microfluidic filter paper chip as claimed in claim 4, wherein the hole pattern of the hydrophilic region of the detection layer is one of circular, square and triangular.
6. The cardiovascular and cerebrovascular disease multi-index joint diagnosis method based on portable scanner combined microfluidic filter paper chip as claimed in claim 5, wherein the diameter or side length of the hydrophilic region hole of the detection layer is not less than 6 mm.
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| CN110951605A (en) * | 2020-02-10 | 2020-04-03 | 福州大学 | Array type paper-based chip capable of being used for 2019-nCoV virus high-throughput detection and manufacturing method thereof |
| CN111751523A (en) * | 2020-06-30 | 2020-10-09 | 清华大学深圳国际研究生院 | Biochemical index detection device based on micro-fluidic chip and smart phone |
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| CN104677896A (en) * | 2015-01-31 | 2015-06-03 | 太原理工大学 | Preparation and application of paper-based microfluidic chip for colorimetric analysis |
| CN109254000A (en) * | 2018-10-25 | 2019-01-22 | 太原理工大学 | Array urine multiple determination apparatus and method based on smart machine colorimetric analysis |
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| CN102998439A (en) * | 2011-09-14 | 2013-03-27 | 佳木斯大学 | Micro-fluidic paper chip for simultaneously detecting glucose, uric acid, triglycerides and cholesterols, and its manufacturing method |
| CN104677896A (en) * | 2015-01-31 | 2015-06-03 | 太原理工大学 | Preparation and application of paper-based microfluidic chip for colorimetric analysis |
| CN109254000A (en) * | 2018-10-25 | 2019-01-22 | 太原理工大学 | Array urine multiple determination apparatus and method based on smart machine colorimetric analysis |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110951605A (en) * | 2020-02-10 | 2020-04-03 | 福州大学 | Array type paper-based chip capable of being used for 2019-nCoV virus high-throughput detection and manufacturing method thereof |
| CN110951605B (en) * | 2020-02-10 | 2020-10-09 | 福州大学 | Array type paper-based chip capable of being used for 2019-nCoV virus high-throughput detection and manufacturing method thereof |
| CN111751523A (en) * | 2020-06-30 | 2020-10-09 | 清华大学深圳国际研究生院 | Biochemical index detection device based on micro-fluidic chip and smart phone |
| CN111751523B (en) * | 2020-06-30 | 2023-03-14 | 清华大学深圳国际研究生院 | Biochemical index detection device based on micro-fluidic chip and smart phone |
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