CN110623633A - Method for detecting human tissue inflammation based on skin autofluorescence and application thereof - Google Patents
Method for detecting human tissue inflammation based on skin autofluorescence and application thereof Download PDFInfo
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- CN110623633A CN110623633A CN201810652903.6A CN201810652903A CN110623633A CN 110623633 A CN110623633 A CN 110623633A CN 201810652903 A CN201810652903 A CN 201810652903A CN 110623633 A CN110623633 A CN 110623633A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0062—Arrangements for scanning
- A61B5/0064—Body surface scanning
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0071—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by measuring fluorescence emission
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/44—Detecting, measuring or recording for evaluating the integumentary system, e.g. skin, hair or nails
- A61B5/441—Skin evaluation, e.g. for skin disorder diagnosis
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention provides a principle and a method for detecting human tissue inflammation based on skin autofluorescence. The method comprises the following steps: (1) detecting the autofluorescence of the skin tissue at the wavelength of 420-800nm under the excitation of the excitation light of 400-700 nm; (2) the intensity of the autofluorescence is used for representing the severity degree of the tissue inflammation, so that the detection method for detecting the severity degree of the tissue inflammation based on the skin autofluorescence is completed. Our research finds that the autofluorescence intensity is positively correlated with the severity of tissue inflammation, so that the real-time noninvasive detection of human tissue inflammation can be achieved by detecting the autofluorescence intensity of skin. According to the strength, the form, the left-right symmetry and other properties of the autofluorescence, the tissue of the patient suffering from inflammation can be judged.
Description
Technical Field
The invention relates to a biological fluorescent substance for detecting human tissue inflammation based on skin autofluorescence, in particular to a method for detecting tissue inflammation based on detecting the autofluorescence intensity of skin tissue and application thereof.
Background
Current methods of detecting tissue inflammation are commonly through blood tests. The accuracy of this approach and the degree of differentiation between different tissue inflammations is limited. Therefore, the method is very important for finding a biological index for real-time noninvasive rapid detection of tissue inflammation, and has great social and economic significance and clinical significance.
Disclosure of Invention
The present inventors have found that a novel biomarker for a method of detecting tissue inflammation can be detected using autofluorescence of skin tissue. Thus, a method is proposed which can be used for the skin autofluorescence-based detection of tissue inflammation.
The invention uses lipopolysaccharide to carry out intraperitoneal injection on a mouse, and induces tissue inflammation. The skin autofluorescence is obviously increased, and the skin autofluorescence is used as a biological index for detecting tissue inflammation. Establishes a novel method for detecting tissue inflammation and application.
The specific technical scheme of the invention is as follows:
1. a method for detecting tissue inflammation based on skin autofluorescence comprises the following steps:
(1) the skin is placed under laser with the wavelength within the range of 400-700nm to excite the self-fluorescence of the skin.
(2) Detecting the distribution and intensity of the autofluorescence emitted by the skin within the range of 420-800 nm;
(3) comparing the distribution and intensity of the autofluorescence with that of healthy skin tissue
(4) Judging whether the tissue inflammation exists or not and the degree of the tissue inflammation, thereby completing the detection method for detecting the tissue inflammation based on the skin autofluorescence.
Further, the tissue inflammation includes, but is not limited to, the following diseases: viral encephalitis, hepatitis, pneumonia, myocarditis, septicemia, systemic fungal infection, and nephritis.
Further, the experimental method does not require any marking of the skin.
Further, in the detection method of the present invention, the wavelength of the excitation light is preferably in the range of 420-680nm, and more preferably in the range of 460-643 nm.
In the detection method of the present invention, the wavelength of the detected autofluorescence is preferably within the range of 440-750nm, and more preferably within the range of 450-741 nm.
Further, the excitation of skin autofluorescence with excitation light includes at least one of excitation with a normal continuous light output, modulation excitation with electrical modulation, or excitation with pulsed laser.
Further, the wavelength of the exciting light is within the range of 400-700 nm.
Further, the wavelength of the self-fluorescence is in the range of 420-800 nm.
Further, the fluorescence intensity is increased compared to the autofluorescence of skin tissue of healthy people.
Further, the fluorescence intensity is increased and positively correlated with the severity of tissue inflammation. The higher the fluorescence intensity, the higher the severity of tissue inflammation.
Further, the various tissue inflammations all have the unique autofluorescence intensity, form and bilateral symmetry. The location of the tissue of the patient suffering from inflammation can be determined from the properties of the autofluorescence, such as intensity, morphology, and bilateral symmetry.
The invention also provides an application of the detection method for detecting the tissue inflammation based on the skin autofluorescence.
Various tissue inflammations have unique autofluorescence intensity, form and bilateral symmetry. The location of the tissue of the patient suffering from inflammation can be determined from the properties of the autofluorescence, such as intensity, morphology, and bilateral symmetry.
Drawings
FIG. 1: the excitation light 488nm excites the skin to self-fluoresce and receives the receiving light with the wavelength range of 500-550 nm. The autofluorescence increase represents the graph.
Detailed Description
The present invention will be further illustrated by the following detailed description.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
The term "autofluorescence" as used herein means the phenomenon in which a biomolecule, when irradiated with excitation light of an appropriate wavelength, absorbs the energy of the excitation light into an excited state and then exits the excited state to emit light of a wavelength longer than that of the excitation light.
The term "excitation light" as used in the present invention means light capable of exciting a biomolecule to undergo autofluorescence, and the wavelength should be shorter than the autofluorescence
The term "tissue inflammation" as used herein, is meant to include, but is not limited to, the following diseases: viral encephalitis, hepatitis, pneumonia, myocarditis, and nephritis. .
The inventors have performed a number of experiments to determine the relationship between skin autofluorescence and tissue inflammation. Where an isochronal determination is required
FIG. 1: mice were treated with lipopolysaccharide and after 12 hours, the mice skin autofluorescence intensity was higher with higher lipopolysaccharide dose.
Example 1:
male C57 mice were used and were housed in an animal house at 22-24℃ for 12 hours light/dark cycles and were allowed free access to water. Injecting lipopolysaccharide into the abdominal cavity of the mouse, and performing non-invasive real-time imaging on the skin of the mouse treated by the lipopolysaccharide by using a laser confocal microscope after 3 hours and 12 hours. Wherein the excitation wavelength of the laser confocal microscope is 440-600 nm.
The content of the inflammatory factors in heart tissues, kidney tissues, lung tissues and brain tissues of the mice is increased within 3 hours and 12 hours by PCR detection.
It was found that the intensity of skin fluorescence can detect the severity of inflammation. Meanwhile, the fluorescence intensity of the skin is found to be positively correlated with the severity of the skin inflammation. The higher the intensity of skin fluorescence, the higher the severity of skin inflammation.
Example 2: the exciting light is blue light wave band, and the autofluorescence intensity of 14 body surfaces is related to tissue inflammation.
The device performs real-time noninvasive imaging on autofluorescence of the skin on arms, palms, finger bellies, dorsal sides of second finger segments of fingers and backs of hands at symmetrical positions of left and right hands of people with viral encephalitis, pneumonia, hepatitis and nephritis and healthy people by using an instrument.
We found that the intensity of skin autofluorescence was significantly higher in the population with tissue inflammation than in the healthy population. Furthermore, the fluorescence intensity, fluorescence pattern, and left-right asymmetry of these 14 sites can be used for joint detection and analysis, the severity of inflammation, the tissue site with inflammation, and the like.
From the above experiment, it can be seen that the autofluorescence of mice treated with lipopolysaccharide changes, and the change is positively correlated with tissue inflammation. Therefore, the skin autofluorescence can be used as a marker for noninvasive real-time detection of tissue inflammation.
Based on the discovery, the invention establishes a method for noninvasive real-time detection of tissue inflammation based on skin autofluorescence, which comprises the following steps:
1. a principle and a method for detecting human tissue inflammation based on skin autofluorescence are disclosed:
(1) the skin is placed under laser with the wavelength within the range of 400-700nm to excite the self-fluorescence of the skin.
(2) Detecting the distribution and intensity of the autofluorescence emitted by the skin within the range of 420-800 nm;
(3) comparing the distribution and intensity of the autofluorescence with that of healthy skin tissue
(4) Judging whether the tissue inflammation exists or not and the degree of the tissue inflammation, thereby completing the detection method for detecting the tissue inflammation based on the skin autofluorescence.
The excitation of skin autofluorescence with excitation light according to the present invention includes at least one of excitation using a normal continuous light output, modulation excitation using electrical modulation, or excitation using pulsed laser.
It will be apparent to those skilled in the art that, although specific embodiments of the invention have been described herein for purposes of illustration, various modifications may be made without deviating from the spirit and scope of the invention. Therefore, the detailed description and examples of the invention should not be construed as limiting the scope of the invention. The invention is limited only by the appended claims. All documents cited in this application are incorporated herein by reference in their entirety.
Claims (7)
1. A method for detecting human tissue inflammation based on skin autofluorescence comprises the following steps:
(1) placing the skin under laser with the wavelength within the range of 400-700nm to excite the autofluorescence of the skin;
(2) detecting the distribution and intensity of the autofluorescence emitted by the skin within the range of 420-800 nm;
(3) comparing the distribution and intensity of the autofluorescence with the distribution and intensity of autofluorescence of healthy skin tissue;
(4) judging whether the human body has the tissue inflammation or not and judging the severity of the tissue inflammation, thereby completing the detection method for detecting the human tissue inflammation based on the skin autofluorescence.
2. The method of claim 1, wherein:
the tissue inflammation includes, but is not limited to, the following diseases: viral encephalitis, hepatitis, pneumonia, myocarditis, septicemia, systemic fungal infection, or nephritis.
3. The method of claim 1, wherein:
the means for exciting the autofluorescence of the skin with the excitation light includes at least one of means for excitation with a normal continuous light output, means for modulated excitation with electrical modulation, or means for excitation with a pulsed laser.
4. The method of claim 1, wherein:
the fluorescence intensity is increased, which can be compared with the autofluorescence of skin tissues of healthy people.
5. The method of claim 1, wherein:
the fluorescence intensity is increased and is positively correlated with the severity of human tissue inflammation; the higher the fluorescence intensity, the higher the severity of inflammation in human tissue.
6. The method of claim 1, wherein:
the position of the tissue of the patient suffering from inflammation is determined based on the properties of the autofluorescence, such as intensity, morphology, and bilateral symmetry.
7. The application of the biomarker for preparing the biomarker for detecting the inflammation of the human tissue is to excite the autofluorescence intensity of the skin on the surface of a tested person by using exciting light between 400nm and 700nm, and the wavelength of the autofluorescence is in the range of 420nm to 800 nm.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113995377A (en) * | 2021-09-16 | 2022-02-01 | 上海交通大学 | Smoking damage assessment device and method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113995377A (en) * | 2021-09-16 | 2022-02-01 | 上海交通大学 | Smoking damage assessment device and method |
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Application publication date: 20191231 |