CN110616144A - Liquid drop digital PCR chip and use method thereof - Google Patents
Liquid drop digital PCR chip and use method thereof Download PDFInfo
- Publication number
- CN110616144A CN110616144A CN201810970247.4A CN201810970247A CN110616144A CN 110616144 A CN110616144 A CN 110616144A CN 201810970247 A CN201810970247 A CN 201810970247A CN 110616144 A CN110616144 A CN 110616144A
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- chip
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- 238000007847 digital PCR Methods 0.000 title claims abstract description 22
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- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 59
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- 238000011304 droplet digital PCR Methods 0.000 claims description 9
- 239000012295 chemical reaction liquid Substances 0.000 claims description 8
- 239000003153 chemical reaction reagent Substances 0.000 claims description 7
- 238000007789 sealing Methods 0.000 claims description 4
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- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 claims description 2
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 claims description 2
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- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
Abstract
Description
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810970247.4A CN110616144A (en) | 2018-08-24 | 2018-08-24 | Liquid drop digital PCR chip and use method thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810970247.4A CN110616144A (en) | 2018-08-24 | 2018-08-24 | Liquid drop digital PCR chip and use method thereof |
Publications (1)
Publication Number | Publication Date |
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CN110616144A true CN110616144A (en) | 2019-12-27 |
Family
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Family Applications (1)
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CN201810970247.4A Pending CN110616144A (en) | 2018-08-24 | 2018-08-24 | Liquid drop digital PCR chip and use method thereof |
Country Status (1)
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CN (1) | CN110616144A (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111729700A (en) * | 2020-07-09 | 2020-10-02 | 墨卓生物科技(上海)有限公司 | Liquid drop detection method adopting dPCR integrated chip |
CN112553063A (en) * | 2020-12-22 | 2021-03-26 | 苏州缔因安生物科技有限公司 | Integrated digital nucleic acid amplification chip based on micro-droplets and use method and application thereof |
CN112630148A (en) * | 2020-12-16 | 2021-04-09 | 海南大学 | Integrated liquid drop digital PCR detection platform |
Citations (9)
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US20080166720A1 (en) * | 2006-10-06 | 2008-07-10 | The Regents Of The University Of California | Method and apparatus for rapid nucleic acid analysis |
CN102527454A (en) * | 2012-01-31 | 2012-07-04 | 复旦大学 | Micro-fluid control drop concentration device for sample enrichment |
KR20130079799A (en) * | 2012-01-03 | 2013-07-11 | 한국과학기술원 | Fabrication method of uniform submicron droplets and polymeric monodiperse particles using microfluidic flow-focusing devices with three-dimensional topography |
CN103343092A (en) * | 2013-07-19 | 2013-10-09 | 中国科学院上海微***与信息技术研究所 | Method for manufacturing digital PCR (polymerase chain reaction) chip based on mineral-oil saturated PDMS (polydimethylsiloxane) material |
US20140127290A1 (en) * | 2012-11-08 | 2014-05-08 | Ohio State Innovation Foundation | Microcapsules Encapsulating Living Cells |
CN105505761A (en) * | 2015-12-21 | 2016-04-20 | 中国科学院苏州生物医学工程技术研究所 | Digital isothermal nucleic acid detecting device and detecting method thereof |
CN105765055A (en) * | 2013-08-27 | 2016-07-13 | 基纽拜奥股份有限公司 | Microfluidic devices and methods of their use |
CN107478629A (en) * | 2017-09-04 | 2017-12-15 | 中国科学院苏州生物医学工程技术研究所 | A kind of large area digital pcr droplet fluorescence high pass amount detecting device and method |
CN107583692A (en) * | 2017-05-23 | 2018-01-16 | 深圳市博瑞生物科技有限公司 | Drop micro-fluidic chip and preparation method thereof |
-
2018
- 2018-08-24 CN CN201810970247.4A patent/CN110616144A/en active Pending
Patent Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20080166720A1 (en) * | 2006-10-06 | 2008-07-10 | The Regents Of The University Of California | Method and apparatus for rapid nucleic acid analysis |
KR20130079799A (en) * | 2012-01-03 | 2013-07-11 | 한국과학기술원 | Fabrication method of uniform submicron droplets and polymeric monodiperse particles using microfluidic flow-focusing devices with three-dimensional topography |
CN102527454A (en) * | 2012-01-31 | 2012-07-04 | 复旦大学 | Micro-fluid control drop concentration device for sample enrichment |
US20140127290A1 (en) * | 2012-11-08 | 2014-05-08 | Ohio State Innovation Foundation | Microcapsules Encapsulating Living Cells |
CN103343092A (en) * | 2013-07-19 | 2013-10-09 | 中国科学院上海微***与信息技术研究所 | Method for manufacturing digital PCR (polymerase chain reaction) chip based on mineral-oil saturated PDMS (polydimethylsiloxane) material |
CN105765055A (en) * | 2013-08-27 | 2016-07-13 | 基纽拜奥股份有限公司 | Microfluidic devices and methods of their use |
CN105505761A (en) * | 2015-12-21 | 2016-04-20 | 中国科学院苏州生物医学工程技术研究所 | Digital isothermal nucleic acid detecting device and detecting method thereof |
CN107583692A (en) * | 2017-05-23 | 2018-01-16 | 深圳市博瑞生物科技有限公司 | Drop micro-fluidic chip and preparation method thereof |
CN107478629A (en) * | 2017-09-04 | 2017-12-15 | 中国科学院苏州生物医学工程技术研究所 | A kind of large area digital pcr droplet fluorescence high pass amount detecting device and method |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111729700A (en) * | 2020-07-09 | 2020-10-02 | 墨卓生物科技(上海)有限公司 | Liquid drop detection method adopting dPCR integrated chip |
CN112630148A (en) * | 2020-12-16 | 2021-04-09 | 海南大学 | Integrated liquid drop digital PCR detection platform |
CN112553063A (en) * | 2020-12-22 | 2021-03-26 | 苏州缔因安生物科技有限公司 | Integrated digital nucleic acid amplification chip based on micro-droplets and use method and application thereof |
CN112553063B (en) * | 2020-12-22 | 2024-03-01 | 苏州缔因安生物科技有限公司 | Micro-droplet-based integrated digital nucleic acid amplification chip and use method and application thereof |
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Legal Events
Date | Code | Title | Description |
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PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20191227 Assignee: Beijing Linke Biotechnology Co.,Ltd. Assignor: BEIJING INTEGRATED BIOSYSTEMS Co.,Ltd. Contract record no.: X2022990000932 Denomination of invention: A Droplet Digital PCR Chip and Its Application License type: Common License Record date: 20221114 |
|
EE01 | Entry into force of recordation of patent licensing contract | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20191227 |
|
RJ01 | Rejection of invention patent application after publication | ||
EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20191227 Assignee: Beijing Linke Biotechnology Co.,Ltd. Assignor: BEIJING INTEGRATED BIOSYSTEMS Co.,Ltd. Contract record no.: X2022990000932 Denomination of invention: A droplet digital PCR chip and its usage method License type: Common License Record date: 20221114 |