CN110612908A - Method for improving variety of Lipu taro - Google Patents

Method for improving variety of Lipu taro Download PDF

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Publication number
CN110612908A
CN110612908A CN201911114138.3A CN201911114138A CN110612908A CN 110612908 A CN110612908 A CN 110612908A CN 201911114138 A CN201911114138 A CN 201911114138A CN 110612908 A CN110612908 A CN 110612908A
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culture medium
lipu
taro
lipu taro
leaf
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黄志君
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001—Culture apparatus for tissue culture
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008—Methods for regeneration to complete plants

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  • Life Sciences & Earth Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses an improvement method of Lipu taro varieties, which mainly comprises the following steps: (1) and (3) disinfecting the leaves: selecting a Lipu taro plant which is free of diseases and insect pests, has multiple leaf buds and plump bud eyes, taking a leaf of the Lipu taro with a leaf stalk, cutting off the leaf, and detoxifying through pretreatment; (2) inducing cluster buds: inoculating the leaves with petioles of the Lipu taro processed in the step (1) to a bud induction culture medium containing thidiazuron and alpha-naphthylacetic acid, wherein the illumination intensity is 2200-; (3) and (3) multiplication of cluster buds: putting the bud induced in the step (2) into a culture medium for improvement; (4) rooting culture: putting the cluster buds cultured in the step (3) into a rooting culture medium for improvement; (5) hardening and transplanting seedlings: and (4) carrying out field hardening seedling transplantation on the rooted test-tube plantlets of the Lipu taros.

Description

Method for improving variety of Lipu taro
Technical Field
The invention relates to the technical field of planting, in particular to an improvement method of a Lipu taro variety.
Background
Lipu taro is produced in Lipu county, Guilin, City. It is big and nutritious. The braised pork is sliced, fried and then clamped in pork to be made into braised pork with brown sauce, has special flavor, is not greasy, and is delicious food for banquet. Cooking, peeling, putting into a hot pot, adding lard, white sugar and a small amount of milk powder, and pressing into taro with sweet, fragrant, soft and sweet taste; mixing with fish, meat, chicken, winter bamboo shoot, Lentinus Edodes, etc., frying with oil, and making into crisp and tasty product.
The fructus Colocasiae Esculentae has effects of invigorating spleen, promoting diuresis, removing toxic substance, and relieving itching. Can reasonably deliver nutrient substances, moisten skin and improve immunity of organism. Lipu taro, also known as betel taro, is the first choice tribute in Guangxi to tribute royal classic at the end of the year. Especially in the early years of Qing dynasty, Qianlong. And special taro is assigned to the 2008 Beijing Olympic Games. With the broadcasting of "phase-cut Liu Luo" television drama, Lipu taro is well known throughout the country.
The litchi-Pu taro belongs to the Araceae, is called Quiko and betel nut taro, is originally a wild taro, is a good variety formed by long-term natural selection and artificial breeding of the wild taro, has a history of 400 years after being artificially cultivated in Lipu county, records that a Fujian carries the taro into Lipu county in the current year, firstly plants the taro in Guantemple in county city, and plants the taro in periphery by radiation, under the special geographic and natural conditions of Lipu county, under the influence of environmental microclimate, local famous and special products which integrate color, fragrance and taste are gradually formed, the quality is far higher than that of taro produced in other places, the betel nut taro produced in Lipu county has a title of the word "Lipu taro", Qing Dynasty is listed as a first-choice royal tribute in Guangxi, and the year in the last county.
According to the record of 3 years of Lipu will: "old Zhiyun: if there are ten jin or so, it is true. But the one who is a pre-approved temple in the city is preferred. When cut open, the lines of betel nut are called betel nut taro. The pattern is brown and dense, loose but not sticky, and fragrant. The seeds moving in other places are only shaped like ears and have no lines, which is called the hammer taro.
Lipu taro is used as a main traditional product in Lipu county and is planted in thirteen villages and towns in the county, and particularly, the Lipu taro and the peeled vegetable are planted in the Qingshan mountain, the New terrace, the Dumo and the peeled vegetable. The traditional Lipu taro is limited by the conditions of climate, temperature and the like of the planting and producing area, so that the taste and yield of the traditional Lipu taro are greatly different and far from the characteristics of the genuine variety. Therefore, we propose a variety improvement method of Lipu taro to be put into use to solve the above problems.
Disclosure of Invention
The invention provides an improvement method of a Lipu taro variety, and the Lipu taro variety prepared by the method has the advantages of high survival rate, low production cost, short growth cycle, good genetic stability and contribution to large-scale popularization and planting.
The specific technical scheme of the invention is as follows:
an improvement method of Lipu taro variety, comprising the following steps of disinfection of leaves, induction of cluster buds, multiplication of the cluster buds, rooting culture and hardening and transplanting, wherein the main steps are as follows:
(1) and (3) disinfecting the leaves: selecting a Lipu taro plant which is free of diseases and insect pests, has multiple leaf buds and plump bud eyes, taking a leaf of the Lipu taro with a leaf stalk, cutting off the leaf, and detoxifying through pretreatment;
(2) inducing cluster buds: inoculating the leaves with petioles of the Lipu taro processed in the step (1) to a bud induction culture medium containing thidiazuron and alpha-naphthylacetic acid, wherein the bud induction culture medium is an MS culture medium, the concentration of the thidiazuron is 2.5-3mg/L, the concentration of the alpha-naphthylacetic acid is 0.3-0.5mg/L, 50g/L of additional sucrose, 10-12g/L of agar, the pH value is 5.5-6.0, the illumination intensity is 2200-;
(3) and (3) multiplication of cluster buds: putting the bud induced in the step (2) into a culture medium for improving DKW +6-BA3.0mg/L + IBA0.2mg/L + PVP4g/L, adding 35g/L of cane sugar, 8.5g/L of agar, pH5.5, illuminating 2000lx and keeping the temperature at 23 +/-5 ℃;
(4) rooting culture: putting the cluster buds cultured by the proliferation in the step (3) into a rooting culture medium improved 1/2DKW, 0.4-0.6mg/L IBA, 0.4-0.6mg/L uniconazole and AgNO35mg/L, 25g/L of added cane sugar, 8g/L of agar, 5.5 of PH, 3000lx of illumination and 23 +/-5 ℃ of temperature;
(5) hardening and transplanting seedlings: and (4) carrying out field hardening seedling transplantation on the rooted test-tube plantlets of the Lipu taros.
It is further explained that the pretreatment detoxification process in step (1) is as follows: collecting branches in a climate of 3 days or more, cutting off leaves, soaking in 5% laundry solution, simultaneously slightly scrubbing surface dirt with a soft brush, washing with running water for 30 min, treating with 75% ethanol for 30 s, washing with sterile water for 3 times, and then washing with 0.1% HgCl2Soaking in the solution for 15 min, washing with sterile water for 2-6 times, each for 2-6 min.
It is further explained that the improved DKW in the rooting culture in the step (4) is 1/2 of the DKW culture medium except for nitrogen salt, and activated carbon is added, and the activated carbon has a certain promotion effect on the rooting of the test-tube plantlet.
It should be further noted that, in the seedling hardening and transplanting method in the step (5), the test-tube seedling with the length of about 5cm is taken out from the culture bottle, the culture medium at the root is washed, and the seedling is planted in perlite: sandy loam = 1: 2, placing the substrate in a greenhouse for culturing, taking out the substrate after one week, selecting a good land, deep ploughing, applying a small amount of farmyard manure, stirring and sterilizing the land with sand by using thiophanate methyl after applying the fertilizer, and counting the survival rate after culturing for 30 days.
It is further explained that before transplanting, the tissue culture seedlings are soaked in rooting water for 1-2 h.
It needs to be further explained that the root fixing water comprises the following components: 3.5 percent of metalaxyl, 3.5 percent of hymexazol, 0.08 percent of indolebutyric acid, 0.08 percent of sodium naphthalene acetate, 0.6 percent of sodium polyacrylate and the balance of purified water.
The outstanding substantive features and remarkable progress of the invention are as follows:
1. the operation is simple: on the basis of an induction culture medium, the culture medium is optimized, so that a large amount of cluster buds can be propagated in a short time;
2. the survival rate of the Lipu taro improved by the method is over 90 percent, the period is short, the pollution is little, the production cost is low, and the method is beneficial to large-scale planting.
Detailed Description
The following detailed description of the preferred embodiments of the invention, while indicating the advantages and features of the invention, will become apparent to those skilled in the art from the following detailed description, which is by way of illustration, specific embodiments of the invention are described.
Example 1:
an improvement method of Lipu taro variety, comprising the following steps of disinfection of leaves, induction of cluster buds, multiplication of the cluster buds, rooting culture and hardening and transplanting, wherein the main steps are as follows:
(1) and (3) disinfecting the leaves: selecting a Lipu taro plant which is free of diseases and insect pests, has multiple leaf buds and plump bud eyes, taking a leaf of the Lipu taro with a leaf stalk, cutting off the leaf, and detoxifying through pretreatment; the pretreatment detoxification process comprises the following steps: collecting branches in a climate of 3 days or more, cutting off leaves, soaking in 5% laundry solution, simultaneously slightly scrubbing surface dirt with a soft brush, washing with running water for 30 min, treating with 75% ethanol for 30 s, washing with sterile water for 3 times, and then washing with 0.1% HgCl2Soaking in the solution for 15 min, washing with sterile water for 2-6 times, each for 2-6 min.
(2) Inducing cluster buds: inoculating the leaves with petioles of the Lipu taro processed in the step (1) to a bud induction culture medium containing thidiazuron and alpha-naphthylacetic acid, wherein the bud induction culture medium is an MS culture medium, the concentration of the thidiazuron is 2.5mg/L, the concentration of the alpha-naphthylacetic acid is 0.3mg/L, 50g/L of added sucrose, 10g/L of agar, 5.5-6.0 of pH, the illumination intensity of 2200-;
(3) and (3) multiplication of cluster buds: putting the bud induced in the step (2) into a culture medium for improving DKW +6-BA3.0mg/L + IBA0.2mg/L + PVP4g/L, adding 35g/L of cane sugar, 8.5g/L of agar, pH5.5, illuminating 2000lx and keeping the temperature at 18 ℃;
(4) rooting culture: putting the cluster buds cultured in the step (3) into a rooting culture medium improved 1/2DKW, IBA0.4mg/L, uniconazole 0.4mg/L and AgNO35mg/L of sucrose, 25g/L of agar, 8g/L of agar, 5.5 of PH, 3000lx of illumination and 18 ℃ of temperature; the improved DKW in the rooting culture is 1/2 of the DKW culture medium except nitrogen salt, and activated carbon is added, and the activated carbon has a certain promotion effect on rooting of the test-tube plantlet.
(5) Hardening and transplanting seedlings: and (4) carrying out field hardening seedling transplantation on the rooted test-tube plantlets of the Lipu taros. The seedling hardening and transplanting method comprises the steps of taking test-tube seedlings with the length of about 5cm out of a culture bottle, washing off a culture medium at the root, and planting the test-tube seedlings in perlite: sandy loam = 1: 2, placing the substrate in a greenhouse for culturing, taking out the substrate after one week, and soaking the tissue culture seedlings in rooting water for 1 hour. The root fixing water comprises the following components: 3.5 percent of metalaxyl, 3.5 percent of hymexazol, 0.08 percent of indolebutyric acid, 0.08 percent of sodium naphthalene acetate, 0.6 percent of sodium polyacrylate and the balance of purified water. Selecting a good land, deep ploughing, applying a small amount of farmyard manure, applying the farmyard manure, stirring and sterilizing the farmyard manure with sandy soil after applying the fertilizer, and counting the survival rate to 90 percent after 30 days.
Example 2:
an improvement method of Lipu taro variety, comprising the following steps of disinfection of leaves, induction of cluster buds, multiplication of the cluster buds, rooting culture and hardening and transplanting, wherein the main steps are as follows:
(1) and (3) disinfecting the leaves: selecting a Lipu taro plant with no disease, no insect pest, multiple leaf buds and plump bud eyes, taking the leaf blade with petiole of Lipu taro, and cuttingThe leaves are detoxified by pretreatment; the pretreatment detoxification process comprises the following steps: collecting branches in a climate of 3 days or more, cutting off leaves, soaking in 5% laundry solution, simultaneously slightly scrubbing surface dirt with a soft brush, washing with running water for 30 min, treating with 75% ethanol for 30 s, washing with sterile water for 3 times, and then washing with 0.1% HgCl2Soaking in the solution for 15 min, washing with sterile water for 2-6 times, each for 2-6 min.
(2) Inducing cluster buds: inoculating the leaves with petioles of the Lipu taro processed in the step (1) to a bud induction culture medium containing thidiazuron and alpha-naphthylacetic acid, wherein the bud induction culture medium is an MS culture medium, the concentration of the thidiazuron is 2.8mg/L, the concentration of the alpha-naphthylacetic acid is 0.4mg/L, 50g/L of added sucrose, 11g/L of agar, 5.5-6.0 of pH, the illumination intensity of 2200-;
(3) and (3) multiplication of cluster buds: putting the bud induced in the step (2) into a culture medium for improving DKW +6-BA3.0mg/L + IBA0.2mg/L + PVP4g/L, adding 35g/L of cane sugar, 8.5g/L of agar, pH5.5, illuminating 2000lx and keeping the temperature at 23 ℃;
(4) rooting culture: putting the cluster buds cultured by the multiplication in the step (3) into a rooting culture medium for improvement of 1/2DKW, IBA0.5mg/L, uniconazole 0.5mg/L and AgNO35mg/L of sucrose, 25g/L of agar, 8g/L of agar, 5.5 of PH, 3000lx of illumination and 23 ℃; the improved DKW in the rooting culture is 1/2 of the DKW culture medium except nitrogen salt, and activated carbon is added, and the activated carbon has a certain promotion effect on rooting of the test-tube plantlet.
(5) Hardening and transplanting seedlings: and (4) carrying out field hardening seedling transplantation on the rooted test-tube plantlets of the Lipu taros. The seedling hardening and transplanting method comprises the steps of taking test-tube seedlings with the length of about 5cm out of a culture bottle, washing off a culture medium at the root, and planting the test-tube seedlings in perlite: sandy loam = 1: 2, placing the substrate in a greenhouse for culturing, taking out the substrate after one week, and soaking the tissue culture seedlings in rooting water for 1.5 hours. The root fixing water comprises the following components: 3.5 percent of metalaxyl, 3.5 percent of hymexazol, 0.08 percent of indolebutyric acid, 0.08 percent of sodium naphthalene acetate, 0.6 percent of sodium polyacrylate and the balance of purified water. Selecting a good land, deep ploughing, applying a small amount of farmyard manure, stirring and sterilizing the land with sandy soil after applying the fertilizer, and counting the survival rate to 95 percent after 30 days.
Example 3:
an improvement method of Lipu taro variety, comprising the following steps of disinfection of leaves, induction of cluster buds, multiplication of the cluster buds, rooting culture and hardening and transplanting, wherein the main steps are as follows:
(1) and (3) disinfecting the leaves: selecting a Lipu taro plant which is free of diseases and insect pests, has multiple leaf buds and plump bud eyes, taking a leaf of the Lipu taro with a leaf stalk, cutting off the leaf, and detoxifying through pretreatment; the pretreatment detoxification process comprises the following steps: collecting branches in a climate of 3 days or more, cutting off leaves, soaking in 5% laundry solution, simultaneously slightly scrubbing surface dirt with a soft brush, washing with running water for 30 min, treating with 75% ethanol for 30 s, washing with sterile water for 3 times, and then washing with 0.1% HgCl2Soaking in the solution for 15 min, washing with sterile water for 2-6 times, each for 2-6 min.
(2) Inducing cluster buds: inoculating the leaves with petioles of the Lipu taro processed in the step (1) to a bud induction culture medium containing thidiazuron and alpha-naphthylacetic acid, wherein the bud induction culture medium is an MS culture medium, the concentration of the thidiazuron is 3mg/L, the concentration of the alpha-naphthylacetic acid is 0.5mg/L, 50g/L of sucrose is added, 12g/L of agar is added, the pH value is 5.5-6.0, the illumination intensity is 2200-;
(3) and (3) multiplication of cluster buds: putting the bud induced in the step (2) into a culture medium for improving DKW +6-BA3.0mg/L + IBA0.2mg/L + PVP4g/L, adding 35g/L of cane sugar, 8.5g/L of agar, pH5.5, illuminating 2000lx and keeping the temperature at 28 ℃;
(4) rooting culture: putting the cluster buds cultured by proliferation in the step (3) into a rooting culture medium improved 1/2DKW + IBA0.6mg/L + uniconazole 0.6mg/L + AgNO35mg/L of sucrose, 25g/L of agar, 8g/L of agar, 5.5 of PH, 3000lx of illumination and 28 ℃ of temperature; the improved DKW in the rooting culture is 1/2 of the DKW culture medium except nitrogen salt, and activated carbon is added, and the activated carbon has a certain promotion effect on rooting of the test-tube plantlet.
(5) Hardening and transplanting seedlings: and (4) carrying out field hardening seedling transplantation on the rooted test-tube plantlets of the Lipu taros. The seedling hardening and transplanting method comprises the steps of taking test-tube seedlings with the length of about 5cm out of a culture bottle, washing off a culture medium at the root, and planting the test-tube seedlings in perlite: sandy loam = 1: 2, placing the substrate in a greenhouse for culturing, taking out the substrate after one week, and soaking the tissue culture seedlings in rooting water for 2 hours. The root fixing water comprises the following components: 3.5 percent of metalaxyl, 3.5 percent of hymexazol, 0.08 percent of indolebutyric acid, 0.08 percent of sodium naphthalene acetate, 0.6 percent of sodium polyacrylate and the balance of purified water. Selecting a good land, deep ploughing, applying a small amount of farmyard manure, stirring and sterilizing the land with sandy soil after applying the fertilizer, and counting the survival rate to 93 percent after 30 days.
It should be noted that the above-mentioned embodiments are only preferred embodiments of the present invention, and the present invention is not limited thereto, and although the present invention is described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and equivalents can be made in the technical solutions described in the foregoing embodiments, or some technical features of the present invention may be substituted. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (6)

1. The improvement method of the Lipu taro variety is characterized by comprising the following main steps of disinfection of leaves, induction of cluster buds, multiplication of the cluster buds, rooting culture and hardening and transplanting:
(1) and (3) disinfecting the leaves: selecting a Lipu taro plant which is free of diseases and insect pests, has multiple leaf buds and plump bud eyes, taking a leaf of the Lipu taro with a leaf stalk, cutting off the leaf, and detoxifying through pretreatment;
(2) inducing cluster buds: inoculating the leaves with petioles of the Lipu taro processed in the step (1) to a bud induction culture medium containing thidiazuron and alpha-naphthylacetic acid, wherein the bud induction culture medium is an MS culture medium, the concentration of the thidiazuron is 2.5-3mg/L, the concentration of the alpha-naphthylacetic acid is 0.3-0.5mg/L, 50g/L of additional sucrose, 10-12g/L of agar, the pH value is 5.5-6.0, the illumination intensity is 2200-;
(3) and (3) multiplication of cluster buds: putting the bud induced in the step (2) into a culture medium for improving DKW +6-BA3.0mg/L + IBA0.2mg/L + PVP4g/L, adding 35g/L of cane sugar, 8.5g/L of agar, pH5.5, illuminating 2000lx and keeping the temperature at 23 +/-5 ℃;
(4) rooting culture: taking the proliferation culture in the step (3) to obtainThe cluster buds are put into a rooting culture medium for improvement of 1/2DKW, 0.4-0.6mg/L IBA, 0.4-0.6mg/L uniconazole and AgNO35mg/L, 25g/L of added cane sugar, 8g/L of agar, 5.5 of PH, 3000lx of illumination and 23 +/-5 ℃ of temperature;
(5) hardening and transplanting seedlings: and (4) carrying out field hardening seedling transplantation on the rooted test-tube plantlets of the Lipu taros.
2. The method for improving variety of Lipu taro as claimed in claim 1, wherein the pretreatment and detoxification process in step (1) comprises: collecting branches in a climate of 3 days or more, cutting off leaves, soaking in 5% laundry solution, simultaneously slightly scrubbing surface dirt with a soft brush, washing with running water for 30 min, treating with 75% ethanol for 30 s, washing with sterile water for 3 times, and then washing with 0.1% HgCl2Soaking in the solution for 15 min, washing with sterile water for 2-6 times, each for 2-6 min.
3. The method for improving the variety of Lipu taro as claimed in claim 1, wherein the DKW improved in the rooting culture in step (4) is 1/2 of the DKW culture medium except for nitrogen salt, and activated carbon is added, and the activated carbon has a certain promotion effect on the rooting of test-tube plantlets.
4. The method for improving the variety of Lipu taro as claimed in claim 1, wherein the hardening transplantation method in step (5) comprises taking test-tube plantlets about 5cm long out of the culture flask, washing off the culture medium at the root, planting the test-tube plantlets on perlite: sandy loam = 1: 2, placing the substrate in a greenhouse for culturing, taking out the substrate after one week, selecting a good land, deep ploughing, applying a small amount of farmyard manure, stirring and sterilizing the land with sand by using thiophanate methyl after applying the fertilizer, and counting the survival rate after culturing for 30 days.
5. The method for improving the variety of Lipu taro as claimed in claim 1, wherein the tissue culture plantlets are soaked in rooting water for 1-2h before transplanting.
6. The method of claim 5, wherein the root water comprises: 3.5 percent of metalaxyl, 3.5 percent of hymexazol, 0.08 percent of indolebutyric acid, 0.08 percent of sodium naphthalene acetate, 0.6 percent of sodium polyacrylate and the balance of purified water.
CN201911114138.3A 2019-11-14 2019-11-14 Method for improving variety of Lipu taro Withdrawn CN110612908A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114467758A (en) * 2022-03-18 2022-05-13 桂林市农业科学研究中心 Method for rapidly proliferating tissue culture seedlings of intermittently immersed Lipu taros

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114467758A (en) * 2022-03-18 2022-05-13 桂林市农业科学研究中心 Method for rapidly proliferating tissue culture seedlings of intermittently immersed Lipu taros

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Application publication date: 20191227