CN110603328A - 定量pcr扩增引物对及其应用 - Google Patents

定量pcr扩增引物对及其应用 Download PDF

Info

Publication number
CN110603328A
CN110603328A CN201780090496.8A CN201780090496A CN110603328A CN 110603328 A CN110603328 A CN 110603328A CN 201780090496 A CN201780090496 A CN 201780090496A CN 110603328 A CN110603328 A CN 110603328A
Authority
CN
China
Prior art keywords
primer
pcr amplification
quantitative pcr
sequence
amplification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201780090496.8A
Other languages
English (en)
Other versions
CN110603328B (zh
Inventor
杨林
高雅
张艳艳
张海萍
黄国栋
陈芳
徐惠欣
蒋慧
徐讯
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BGI Shenzhen Co Ltd
Original Assignee
BGI Shenzhen Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BGI Shenzhen Co Ltd filed Critical BGI Shenzhen Co Ltd
Publication of CN110603328A publication Critical patent/CN110603328A/zh
Application granted granted Critical
Publication of CN110603328B publication Critical patent/CN110603328B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

提供了定量PCR扩增引物对，其包括：第一引物和第二引物，其中，所述第一引物包含第一特异性序列和第一随机序列，所述第一特异性序列位于所述第一引物的3’端，所述第一随机序列位于所述第一引物的5’端，所述第二引物包含第二特异性序列和第二随机序列，所述第二特异性序列位于所述第二引物的3’端，所述第二随机序列位于所述第二引物的5’端，并且，第一特异性序列和第二特异性序列分别为针对靶序列的上游引物和下游引物，第一随机序列和第二随机序列反向互补，所述第一引物的5’末端碱基和所述第二引物的5’末端碱基均连接有荧光基团，且距离所述第一引物的5’末端碱基和所述第二引物的5’末端碱基特定长度的碱基均连接有淬灭基团。

Description

PCT国内申请，说明书已公开。

Claims (13)

  1. PCT国内申请，权利要求书已公开。
CN201780090496.8A 2017-06-20 2017-06-20 定量pcr扩增引物对及其应用 Active CN110603328B (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2017/089197 WO2018232596A1 (zh) 2017-06-20 2017-06-20 定量pcr扩增引物对及其应用

Publications (2)

Publication Number Publication Date
CN110603328A true CN110603328A (zh) 2019-12-20
CN110603328B CN110603328B (zh) 2024-01-12

Family

ID=64736263

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201780090496.8A Active CN110603328B (zh) 2017-06-20 2017-06-20 定量pcr扩增引物对及其应用

Country Status (2)

Country Link
CN (1) CN110603328B (zh)
WO (1) WO2018232596A1 (zh)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114875116A (zh) * 2022-04-27 2022-08-09 广州博懿瑞生物科技有限公司 一种自淬灭荧光的引物及其设计方法及应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2512631A (en) * 2013-04-03 2014-10-08 Rupert Maxwell Gaut Quantitative detection of specific nucleic acid sequences
CN104404142A (zh) * 2014-11-11 2015-03-11 中国科学院上海微***与信息技术研究所 一种用于荧光定量pcr反应的荧光探针
US20160237472A1 (en) * 2012-11-30 2016-08-18 Beijing Tag-Array Molecular Test Co., Ltd Primer middle sequence interference pcr technology
US20160369327A1 (en) * 2013-06-18 2016-12-22 Lgc Limited Fluorophore-based oligonucleotide probes with a universal element

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106755379B (zh) * 2016-12-13 2021-06-04 海南医学院 对4种曲霉菌同步定量和基因分型的二聚体突变荧光引物定量pcr方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160237472A1 (en) * 2012-11-30 2016-08-18 Beijing Tag-Array Molecular Test Co., Ltd Primer middle sequence interference pcr technology
GB2512631A (en) * 2013-04-03 2014-10-08 Rupert Maxwell Gaut Quantitative detection of specific nucleic acid sequences
US20160369327A1 (en) * 2013-06-18 2016-12-22 Lgc Limited Fluorophore-based oligonucleotide probes with a universal element
CN104404142A (zh) * 2014-11-11 2015-03-11 中国科学院上海微***与信息技术研究所 一种用于荧光定量pcr反应的荧光探针

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
HUANTING LIU 等: "Placental exosomes in normal and complicated pregnancy", BMC BIOTECHNOL ., pages 130 - 134 *
JURATE BITINAITE等: "USER™ friendly DNA engineering and cloning method by uracil excision", NUCLEIC ACIDS RES, vol. 35, no. 6, pages 1992 - 2002, XP055170276, DOI: 10.1093/nar/gkm041 *
郭秋平等: "用荧光双链引物特异扩增并定量核酸", 《厦门大学学报(自然科学版)》 *
郭秋平等: "用荧光双链引物特异扩增并定量核酸", 《厦门大学学报(自然科学版)》, vol. 41, no. 01, 10 February 2002 (2002-02-10), pages 108 - 111 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114875116A (zh) * 2022-04-27 2022-08-09 广州博懿瑞生物科技有限公司 一种自淬灭荧光的引物及其设计方法及应用
CN114875116B (zh) * 2022-04-27 2023-08-29 广州博懿瑞生物科技有限公司 一种自淬灭荧光的引物及其设计方法及应用

Also Published As

Publication number Publication date
WO2018232596A1 (zh) 2018-12-27
CN110603328B (zh) 2024-01-12

Similar Documents

Publication Publication Date Title
JP6571895B1 (ja) 核酸プローブ及びゲノム断片検出方法
EP2534263B1 (en) Methods and compositions for universal detection of nucleic acids
US20210230670A1 (en) Solid phase nucleic acid target capture and replication using strand displacing polymerases
SG172965A1 (en) Single-cell nucleic acid analysis
JP3844996B2 (ja) 反復性ｐｃｒ産物の融解曲線解析方法
CN110878356A (zh) 一种多重核酸指数扩增探针及其肿瘤多靶标检测应用
US20180237853A1 (en) Methods, Compositions and Kits for Detection of Mutant Variants of Target Genes
CN112852927A (zh) 一种基于荧光自抑制探针的等温扩增***及方法
US8642264B2 (en) Method of quantifying target and reference nucleic acid
EP4083229A1 (en) Methods and compositions for nucleic acid detection
US20200407794A1 (en) Molecular targets for fetal nucleic acid analysis
CN110603328B (zh) 定量pcr扩增引物对及其应用
EP3447145B1 (en) Target nucleic acid sequence detection method using multiple amplification nested signal amplification
EP3377652B1 (en) A method and a kit for nucleic acid detection
CN110612355B (zh) 用于定量pcr扩增的组合物及其应用
JP2024515076A (ja) 一本鎖ｄｎａの増幅
US20090305288A1 (en) Methods for amplifying nucleic acids and for analyzing nucleic acids therewith
WO2024063036A1 (ja) ゲノムｄｎａのメチル化検出法
JP2020516274A (ja) ライブラリーの定量および定性
WO2023067110A1 (en) Methods and compositions for detection of mutant nucleic acid sequences
EP3377651B1 (en) A method and a kit for nucleic acid detection
Andersen Optimal Design of PCR Assays for Circulating DNA
CN116334185A (zh) 一种基于PER-Cas12a的核酸检测试剂盒
JP2024523655A (ja) 核酸分析のための方法および組成物
WO2024015999A1 (en) Methods, systems and compositions for detection of multiple analytes

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant