CN110596362A - Shellfish oxygen consumption rate continuous measuring device and method - Google Patents
Shellfish oxygen consumption rate continuous measuring device and method Download PDFInfo
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- CN110596362A CN110596362A CN201910711808.3A CN201910711808A CN110596362A CN 110596362 A CN110596362 A CN 110596362A CN 201910711808 A CN201910711808 A CN 201910711808A CN 110596362 A CN110596362 A CN 110596362A
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- 235000015170 shellfish Nutrition 0.000 title claims abstract description 66
- 230000036284 oxygen consumption Effects 0.000 title claims abstract description 46
- 238000000034 method Methods 0.000 title claims abstract description 21
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 65
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 65
- 239000001301 oxygen Substances 0.000 claims abstract description 65
- 230000029058 respiratory gaseous exchange Effects 0.000 claims abstract description 64
- 238000005259 measurement Methods 0.000 claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 241001339782 Scapharca broughtonii Species 0.000 claims description 6
- 238000012417 linear regression Methods 0.000 claims description 6
- 241000195493 Cryptophyta Species 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 4
- 210000004872 soft tissue Anatomy 0.000 claims description 4
- 238000005266 casting Methods 0.000 claims description 3
- 241000237536 Mytilus edulis Species 0.000 claims description 2
- 238000001514 detection method Methods 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 238000002474 experimental method Methods 0.000 description 4
- 230000002706 hydrostatic effect Effects 0.000 description 3
- 230000004060 metabolic process Effects 0.000 description 3
- 230000035790 physiological processes and functions Effects 0.000 description 3
- 239000013535 sea water Substances 0.000 description 3
- 230000006978 adaptation Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 241000628923 Anadara sativa Species 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000003760 magnetic stirring Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/54—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Environmental Sciences (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Animal Husbandry (AREA)
- Biophysics (AREA)
- Hematology (AREA)
- Zoology (AREA)
- Urology & Nephrology (AREA)
- Marine Sciences & Fisheries (AREA)
- Food Science & Technology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to a shellfish oxygen consumption rate continuous measurement device and method, the device comprises a breathing bottle, a porous plug, a dissolved oxygen sensor, a bait tube, a dissolved oxygen measurement instrument, a magnetic rotor and a magnetic stirrer, wherein the breathing bottle is arranged on the magnetic stirrer, the magnetic rotor is arranged in the breathing bottle, the porous plug seals the mouth of the breathing bottle, the dissolved oxygen sensor and the bait tube are respectively inserted on the porous plug, and the dissolved oxygen sensor is connected with the dissolved oxygen measurement instrument. The determination method is to calculate the shellfish oxygen consumption rate by measuring the rate of the oxygen concentration decrease in the breathing bottle. The invention can realize the continuous detection of the shellfish oxygen consumption rate and is beneficial to improving the accuracy of the measurement result.
Description
Technical Field
The invention belongs to the technical field of shellfish oxygen consumption determination, and particularly relates to a shellfish oxygen consumption rate continuous determination device and method.
Background
The oxygen consumption rate reflects the physiological state of the shellfish, is one of important indexes of shellfish metabolism, and can be used for evaluating the adaptability of the shellfish to environmental conditions; meanwhile, the oxygen consumption rate is also an important parameter for constructing a shellfish physiological energy model, and has important significance for shellfish culture capacity evaluation.
The shellfish oxygen consumption rate measuring method mainly comprises a flow method and a hydrostatic method, and the hydrostatic method is still the method adopted by most researchers at present. At present, the oxygen consumption rate of shellfish is mainly determined by a hydrostatic method, namely, the shellfish is put into a breathing bottle, and after the shellfish is sealed for a period of time, the oxygen consumption rate is calculated according to the concentration of dissolved oxygen in the breathing bottle and the volume of the breathing bottle before and after an experiment. This method is simple to operate, but has a number of problems including: (1) the shellfish is put into the breathing bottle and then sealed, operation stress exists, the shellfish may not be in a normal physiological state during the experiment, the adaptation time is different due to different shellfish types, sizes and states, the adaptation time cannot be accurately deducted, and the accuracy of the result is influenced; (2) the experimental result only has initial and end data, and the physiological state change of the shellfish during the experiment can not be accurately known. Therefore, it is necessary to develop a new shellfish oxygen consumption rate measuring apparatus, to realize continuous measurement, and to improve the accuracy of the result.
A conventional oxygen consumption rate measuring apparatus includes: "a simple closed water flow device for measuring oxygen consumption" (wuweidong, 2001) which is designed to keep the velocity of the water flowing out constant by siphonage, is mainly used for measuring the oxygen consumption of fishes, and is not related to shellfish. A device for measuring the oxygen consumption rate of seawater shellfish (201320713686.X) adopts a water flowing method to measure the oxygen consumption rate of seawater shellfish. A system and a method for measuring the metabolism and physiology of marine organisms (2015104243967) are provided, the device adopts a multi-channel dissolved oxygen measuring instrument to measure the concentration of dissolved oxygen in a breathing bottle, seawater in the breathing bottle is replaced under the control of a water pump and a flow divider, but whether the dissolved oxygen in the breathing bottle is uniformly distributed after sealing cannot be determined.
Disclosure of Invention
The invention aims to provide a device and a method for continuously measuring shellfish oxygen consumption rate, which realize continuous detection of shellfish oxygen consumption rate and improve accuracy of measurement results.
The technical scheme adopted by the invention for solving the technical problems is to provide a shellfish oxygen consumption rate continuous measuring device which comprises a breathing bottle, a porous plug, a dissolved oxygen sensor, a bait tube, a dissolved oxygen measuring instrument, a magnetic rotor and a magnetic stirrer, wherein the breathing bottle is placed on the magnetic stirrer, the magnetic rotor is arranged in the breathing bottle, the porous plug seals the mouth of the breathing bottle, the dissolved oxygen sensor and the bait tube are respectively inserted on the porous plug, and the dissolved oxygen sensor is connected with the dissolved oxygen measuring instrument.
The breathing bottle is a wide-mouth bottle, the dissolved oxygen sensor is located at the center of the porous plug, and the lower end of the dissolved oxygen sensor extends into 4-5 cm below the porous plug.
The upper end of the bait pipe is connected with a hose, and the hose is clamped and sealed through a clamp.
The dissolved oxygen measuring instrument can set a measurement time interval when measuring the dissolved oxygen concentration.
The technical scheme adopted by the invention for solving the technical problem is to provide a shellfish oxygen consumption rate continuous measuring method, which uses the shellfish oxygen consumption rate continuous measuring device and comprises the following steps:
(1) filling the breathing bottle with fully aerated filtered water, and placing the shellfish to be detected close to the inner wall of the bottom of the breathing bottle;
(2) inserting the dissolved oxygen sensor and the bait tube into the porous plug, and plugging the porous plug into the mouth of the breathing bottle to ensure sealing;
(3) adjusting a magnetic rotor in the breathing bottle to the central position of the bottom of the breathing bottle, starting a magnetic stirrer, and adjusting the rotating speed according to the size of the shellfish to be detected;
(4) starting a dissolved oxygen tester, setting a reading time interval according to the number and the individual size of the shellfish to be tested in the breathing bottle, and recording the concentration of dissolved oxygen;
(5) repeating the steps (3) and (4) for more than three times, and recording the dissolved oxygen concentration of each experimental group at each time point;
(6) setting a blank control group, and measuring the dissolved oxygen concentration of the respiration bottle without shellfish at each time point;
(7) correcting the experimental group data by using blank control group data, and calculating the average value of the dissolved oxygen concentration at each time point;
(8) and (4) taking the average value of the dissolved oxygen concentration obtained in the step (7) as a linear regression graph of the dissolved oxygen concentration and time, calculating the slope b of a linear regression line, measuring the volume V of the breathing bottle, measuring the dry weight w of the shellfish soft tissue, and calculating the oxygen consumption rate R of the shellfish to be measured according to the value of R ═ b multiplied by V/w.
In the step (4), bait casting is not required when the basic oxygen consumption of the shellfish is measured; when the oxygen consumption of shellfish under different bait concentrations is measured, unicellular algae bait is injected into the breathing bottle (1) through the bait pipe (4) according to the volume of the breathing bottle (1).
The shellfish to be detected is clam, common mussel or blood clam.
Advantageous effects
In the invention, the dissolved oxygen in the breathing bottle can be uniformly distributed through magnetic stirring, and the dissolved oxygen sensor can measure the change of the concentration of the dissolved oxygen in the breathing bottle in real time, so that the shellfish oxygen consumption rate can be continuously and accurately measured. The invention can add unicellular algae baits with different concentrations according to the needs, can realize the shellfish basic metabolism oxygen consumption rate and the shellfish oxygen consumption rate under different bait concentrations, and improves the functionality. Moreover, the device of the invention has simple and convenient operation, simple structure, small volume and convenient carrying, and can measure the shellfish oxygen consumption rate in field when being provided with a mobile power supply.
Drawings
FIG. 1 is a schematic structural diagram of the present invention.
Detailed Description
The invention will be further illustrated with reference to the following specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Further, it should be understood that various changes or modifications of the present invention may be made by those skilled in the art after reading the teaching of the present invention, and such equivalents may fall within the scope of the present invention as defined in the appended claims.
The continuous measuring device for the shellfish oxygen consumption rate shown in figure 1 comprises a breathing bottle 1, a porous plug 2, a dissolved oxygen sensor 3, a bait pipe 4, a dissolved oxygen measuring instrument 5, a magnetic rotor 6 and a magnetic stirrer 7.
The breathing bottle 1 is a glass wide-mouth bottle, the diameter of the top bottleneck is half of the diameter of the bottle body, the porous plug 2 is made of rubber, and two holes are formed in the plug and are respectively used for inserting the dissolved oxygen sensor 3 and the bait pipe 5. The breathing bottle 1 is placed on the magnetic stirrer 7, the magnetic rotor 6 is placed inside the breathing bottle 1, and the magnetic rotor 6 is positioned at the center of the breathing bottle 1 during stirring.
The porous plug 2 is plugged at the mouth of the breathing bottle 1 and ensures the sealing of the breathing bottle 1. The dissolved oxygen sensor 3 and the bait pipe 4 are respectively inserted on the porous plug 2, the dissolved oxygen sensor 3 is positioned at the central position of the porous plug 2, the lower end of the dissolved oxygen sensor extends into 4-5 cm below the porous plug 2, the upper end of the bait pipe 4 is connected with a plastic hose, and the hose is clamped and sealed by a clamp. The dissolved oxygen sensor 3 is connected to the dissolved oxygen meter 5, and the measurement time interval can be set when the dissolved oxygen meter 5 measures the dissolved oxygen concentration.
The method for continuously measuring the shellfish oxygen consumption rate comprises the following steps:
(1) at the beginning of the experiment, 2.5L of the breathing bottle 1 is filled with the filtered water which is fully aerated, 6 blood clam shells with the length of about 4cm are placed in the breathing bottle 1, the blood clam shells are adjusted to be close to the inner wall of the bottom of the breathing bottle 1 by a glass rod, and the magnetic rotor 6 is placed in the breathing bottle.
(2) The dissolved oxygen sensor 3 and the bait tube 4 are inserted into the porous plug 2, and then the porous plug 2 is plugged into the mouth of the breathing bottle 1, so that the breathing bottle is tightly attached without air leakage and air bubbles in water.
(3) Place the breathing bottle 1 on magnetic stirrers 7 to with the central point that inside magnetic rotor 6 adjusted to breathing bottle 1 bottom put, open magnetic stirrers 7, according to the individual size adjustment rotational speed of blood clam, the blood clam that uses the adherence to place does not remove along with rivers for the limit.
(4) The dissolved oxygen meter 5 is started, the measurement time is set to be 2 hours, the reading interval time is set to be 5 minutes, the dissolved oxygen concentration in the breathing bottle 1 is measured, and data are recorded. In the process, bait casting is not required when the basic oxygen consumption of the shellfish is measured; when the oxygen consumption of the shellfish under different bait concentrations is measured, a proper amount of unicellular algae bait is injected into the breathing bottle 1 through the bait pipe 4 according to the volume of the breathing bottle 1.
(5) Steps (3) and (4) were repeated three times, and the dissolved oxygen concentration at each time point of each experimental group was recorded.
(6) A blank control group was set up and the dissolved oxygen concentration at each time point in 2 hours in the respiratory flask 1 without shellfish was measured.
(7) The blank control group data are used for correcting the experimental group data respectively and then calculating the average value of the dissolved oxygen concentration at each time point.
(8) And (3) taking the average value of the dissolved oxygen concentration obtained in the step (7) as a linear regression graph of the dissolved oxygen concentration and time, calculating the slope b of a linear regression line, measuring the volume V of the breathing bottle 1, dissecting the scapharca subcrenata in the breathing bottle 1, taking the soft tissue, drying for 24 hours at 65 ℃, weighing to obtain the dry weight w of the shellfish soft tissue, and calculating the oxygen consumption rate R of the shellfish to be measured according to the value of b multiplied by V/w.
Claims (7)
1. The utility model provides a shellfish oxygen consumption rate continuous measurement device, includes breathing bottle (1), porous stopper (2), dissolved oxygen sensor (3), bait pipe (4), dissolved oxygen apparatus (5), magnetic rotor (6) and magnetic stirrers (7), its characterized in that: the respiration bottle (1) is placed on a magnetic stirrer (7), the magnetic rotor (6) is arranged inside the respiration bottle (1), the porous plug (2) seals the mouth of the respiration bottle (1), the dissolved oxygen sensor (3) and the bait tube (4) are respectively inserted on the porous plug (2), and the dissolved oxygen sensor (3) is connected with a dissolved oxygen tester (5).
2. The shellfish oxygen consumption rate continuous measurement apparatus as set forth in claim 1, characterized in that: the breathing bottle (1) is a wide-mouth bottle, the dissolved oxygen sensor (3) is located at the center of the porous plug (2) and the lower end of the dissolved oxygen sensor extends into the position 4-5 cm below the porous plug (2).
3. The shellfish oxygen consumption rate continuous measurement apparatus as set forth in claim 1, characterized in that: the upper end of the bait pipe (4) is connected with a hose which is clamped and sealed by a clamp.
4. The shellfish oxygen consumption rate continuous measurement apparatus as set forth in claim 1, characterized in that: the dissolved oxygen measuring instrument (5) can set a measurement time interval when measuring the dissolved oxygen concentration.
5. A method for continuously measuring shellfish oxygen consumption rate, characterized by using the shellfish oxygen consumption rate continuous measurement apparatus according to any one of claims 1 to 4, comprising the steps of:
(1) fully aerating the filtered water in the breathing bottle (1), and placing the shellfish to be detected close to the inner wall of the bottom of the breathing bottle (1);
(2) inserting the dissolved oxygen sensor (3) and the bait pipe (4) into the porous plug (2), and plugging the porous plug (2) to the mouth of the breathing bottle (1) to ensure sealing;
(3) adjusting a magnetic rotor (6) in the breathing bottle (1) to the central position of the bottom of the breathing bottle (1), starting a magnetic stirrer (7), and adjusting the rotating speed according to the size of the shellfish to be detected;
(4) starting a dissolved oxygen tester (5), setting a reading time interval according to the number and the individual size of the shellfish to be tested in the breathing bottle (1), and recording the concentration of dissolved oxygen;
(5) repeating the steps (3) and (4) for more than three times, and recording the dissolved oxygen concentration of each experimental group at each time point;
(6) setting a blank control group, and measuring the dissolved oxygen concentration of the respiration bottle (1) without shellfish at each time point;
(7) correcting the experimental group data by using blank control group data, and calculating the average value of the dissolved oxygen concentration at each time point;
(8) and (3) taking the average value of the dissolved oxygen concentration obtained in the step (7) as a linear regression graph of the dissolved oxygen concentration and time, calculating the slope b of a linear regression line, measuring the volume V of the breathing bottle (1), measuring the dry weight w of the shellfish soft tissue, and calculating the oxygen consumption rate R of the shellfish to be measured according to the R ═ b × V/w.
6. The method for continuously measuring shellfish oxygen consumption rate according to claim 5, characterized in that: in the step (4), bait casting is not required when the basic oxygen consumption of the shellfish is measured; when the oxygen consumption of shellfish under different bait concentrations is measured, unicellular algae bait is injected into the breathing bottle (1) through the bait pipe (4) according to the volume of the breathing bottle (1).
7. The method for continuously measuring shellfish oxygen consumption rate according to claim 5, characterized in that: the shellfish to be detected is clam, common mussel or blood clam.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201910711808.3A CN110596362A (en) | 2019-08-02 | 2019-08-02 | Shellfish oxygen consumption rate continuous measuring device and method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910711808.3A CN110596362A (en) | 2019-08-02 | 2019-08-02 | Shellfish oxygen consumption rate continuous measuring device and method |
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| CN110596362A true CN110596362A (en) | 2019-12-20 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112931311A (en) * | 2021-02-09 | 2021-06-11 | 上海海洋大学 | Flounder health condition evaluation method |
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2019
- 2019-08-02 CN CN201910711808.3A patent/CN110596362A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112931311A (en) * | 2021-02-09 | 2021-06-11 | 上海海洋大学 | Flounder health condition evaluation method |
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Application publication date: 20191220 |
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