CN110592107B - 一种燕麦叶枯病菌基因CvDUF1及其应用 - Google Patents
一种燕麦叶枯病菌基因CvDUF1及其应用 Download PDFInfo
- Publication number
- CN110592107B CN110592107B CN201910950510.8A CN201910950510A CN110592107B CN 110592107 B CN110592107 B CN 110592107B CN 201910950510 A CN201910950510 A CN 201910950510A CN 110592107 B CN110592107 B CN 110592107B
- Authority
- CN
- China
- Prior art keywords
- gene
- cvduf1
- oat
- leaf blight
- toxin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/136—Screening for pharmacological compounds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/37—Assays involving biological materials from specific organisms or of a specific nature from fungi
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Urology & Nephrology (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Mycology (AREA)
- Food Science & Technology (AREA)
- Botany (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Peptides Or Proteins (AREA)
Abstract
一种燕麦叶枯病菌CvDUF1基因及其应用属微生物基因工程技术领域,本发明提供的来自燕麦叶枯病菌的控制毒素形成的CvDUF1基因,其DNA序列如SEQ ID No:1所示;CvDUF1基因可在植物抗燕麦叶枯病基因工程领域中应用;通过对燕麦叶枯病菌的控制毒素形成的蛋白质CvDUF1进行缺失、突变或修饰,而使其毒素形成受限,可作为靶标在设计和筛选抗燕麦叶枯病药剂中应用。
Description
技术领域
本发明属微生物基因工程技术领域,具体涉及植物保护领域中控制真菌毒素形成的基因的发现及应用。
背景技术
燕麦叶枯病是由一种平脐蠕孢菌Cochliobolusvictoriae引起,燕麦叶枯病主要危害叶片、叶鞘、颖等地上部。叶片染病初期会形成边缘不明显的黄色至黄褐色病斑,后逐渐扩大,中间褐色,四周略带黄色。病斑多时,从叶尖端逐渐向下枯死。该病多从下部叶片开始发生并向上扩展。并且,燕麦叶枯病菌可以产生一种毒素-victorin,毒素victorin稀释一百万倍以后仍对特定基因型的燕麦品种有毒害作用,从此也提出了寄主专化性毒素的概念,并得到广泛研究。毒素victorin是燕麦叶枯病菌成功侵染燕麦的必要元素之一。不能产生victorin的菌株则不能对燕麦产生致病性,而离体的victorin也能侵染燕麦。燕麦对victorin的感病性主要由燕麦Vb基因决定,含有Vb基因的燕麦表现为感病,不含有Vb基因的燕麦表现为抗病。而Vb基因与燕麦抗锈病的Pc2基因是同一个基因,因此燕麦叶枯病的感病性是与一个抗性基因相关联的。并且victorin能够引起燕麦的防卫反应,包括胼胝质积累,氧爆发以及细胞程序性死亡。而victorin主要包括5种结构,分别是B、C、D、E和Victoricine,结构C为主要结构,活性占比达到85%-90%。燕麦叶枯病每年给燕麦生产造成了极大的损失,尤其毒素victorin的产生给人畜安全产生很大影响。而毒素victorin的产生机制目前还不清楚,这给燕麦叶枯病的防治造成了很大的困难。
DUF1(Domain of Unknown Function 1)基因是一个在燕麦叶枯病菌中的未知功能基因,通过分析DUF1基因的功能,评价其在燕麦叶枯病菌毒素产生中的作用,有利于鉴定潜在的防治靶标,用于筛选新型防控燕麦叶枯病菌的药剂。
发明内容
本发明的目的旨在提供一种控制真菌毒素形成的基因。
本发明所提供的控制毒素形成的基因来源于燕麦叶枯病菌(Cochliobolusvictoriae)菌株FI3,名称为CvDUF1,其DNA序列如SEQ ID No:1所示。该DNA序列为CvDUF1基因的开放阅读框,由1004个核苷酸组成,其中包含3个内含子序列。
本发明所述燕麦叶枯病菌(Cochliobolus victoriae)CvDUF1基因可在调控燕麦叶枯病菌毒素产生中应用。
对来自燕麦叶枯病菌(Cochliobolusvictoriae)菌株FI3的控制毒素形成的CvDUF1基因进行缺失、突变或修饰,而使其毒素形成受阻,可作为靶标在设计和筛选抗燕麦叶枯病药剂中应用。
本发明证明了CvDUF1基因的缺失,导致燕麦叶枯病菌毒素不能形成,说明CvDUF1基因是燕麦叶枯病菌(Cochliobolus victoriae)菌株FI3毒素形成所必需的基因。目前,关于CvDUF1基因影响毒素形成方面还没有报道,本研究是第一次报道。因此,筛选能够阻止该基因表达与其蛋白质的表达、修饰及定位的化合物,可以有效控制燕麦叶枯病的发生,从而有助于开发新型杀菌剂,即本发明所提供的CvDUF1基因的一个重要用途是:该基因的表达与其编码的蛋白质产物的表达、修饰及定位,可以作为重要候选靶标位点,用于抗燕麦叶枯病药剂的设计和筛选。
附图说明
图1为燕麦叶枯病菌(Cochliobolus victoriae)菌株FI3CvDUF1基因的敲除策略(通过同源重组进行基因替换)示意图
其中:Cv FI3为燕麦叶枯病菌野生型菌株,ΔCvduf1为CvDUF1基因的缺失突变体;引物F1/R1与F2/R2分别用于扩增CvDUF1基因的上下游序列,用作敲除的同源臂;引物F/R,U/NLC37,NLC38/D用于验证突变体。
图2为CvDUF1基因缺失突变体的PCR验证电泳图
其中:F/R、U/NLC37、D/NLC38为所用引物;1为燕麦叶枯病菌(Cochliobolusvictoriae)FI3野生型菌株,2和3为CvDUF1基因缺失突变体;(1)F/R为部分CvDUF1基因扩增结果,(2)U/NLC37为CvDUF1基因上游序列加部分潮霉素序列扩增结果,(3)D/NLC38为CvDUF1基因下游序列加部分潮霉素序列扩增结果。
图3为CvDUF1基因的缺失突变体、野生型菌株及阴性对照菌株(不能产生毒素菌株)的毒素产生测定对比照片
其中:所用试管中液体为各个菌株的培养液,所用叶片为燕麦幼苗叶片,如果菌株能产生毒素,经过24h培养后燕麦叶片会发生弯曲。1为燕麦叶枯病菌(Cochliobolusvictoriae)FI3野生型菌株,2和3为不能产生毒素的燕麦叶枯病菌菌株(阴性对照),4和5为CvDUF1基因的缺失突变体菌株。
图4为CvDUF1基因的缺失突变体与野生型菌株产生毒素的ESI-EIC谱图
其中:将各个菌株接种在液体Fries’培养基培养15天后,进行毒素测定。标准品为纯毒素标准品,WT为燕麦叶枯病菌野生型菌株,ΔCvDUF1为CvDUF1基因的缺失突变体菌株。
具体实施方式
为了更好地描述本发明,下面通过具体的实施例予以进一步说明,下述实施例中的方法,如无特别说明,均为常规方法。
本发明中燕麦叶枯病菌(Cochliobolusvictoriae)菌株FI3提供者为美国康奈尔大学的Babara Gillian Turgeon。
提供者联系方式为:
Dept.of Plant Pathology&Plant-Microbe Biology,334Plant Science Bldg.,Cornell University,Ithaca,NY 14850,United States.E-mail:[email protected]。
实施例1 CvDUF1基因的相关性分析
CvDUF1基因是通过对燕麦叶枯病菌基因组分析获得。燕麦叶枯病菌CvDUF1基因的开放阅读框由1004个核苷酸组成,包含3个内含子。
实施例2CvDUF1基因的敲除
1)CvDUF1基因上下游以及潮霉素基因的扩增
采用引物F1(5'-GGAGCATGCATTTCTCTACGA-3')与R1(5'-TCCTGTGTGAAATTGTTATCCGCTTGGTATCAAAAGAAAAACAACTGAA-3'),以燕麦叶枯病菌野生型菌株FI3的基因组DNA为模板扩增CvDUF1基因上游702bp片段,采用F2(5'-GTCGTGACTGGGAAAACCCTGGCGTGCTACGAGCAGCAGCTAAA-3')与R2(5'-ATGGTGGAGCTGATTTCTGG-3')扩增燕麦叶枯病菌CvDUF1基因下游850bp片段,采用引物M13F(5'-CGCCAGGGTTTTCCCAGTCACGAC-3')和M13R(5'-AGCGGATAACAATTTCACACAGGA-3'),以载体pUCATPH为模板扩增2549bp潮霉素基因。反应体系为:10mmol/L dNTP Mixture,1μL;5×PCR buffer,10μL;上下游引物各2.5μL(10μmol/mL);模板DNA,2μL;Phusion polymerase,0.5μL(5U);ddH2O,31.5μL;扩增程序为:98℃预变性2分钟,然后(1)98℃,变性20秒;(2)65℃,退火30秒;(3)72℃,延伸30秒;(4)循环30次;(5)72℃延伸10分钟。将上述3个片段共同转入燕麦叶枯病菌野生型菌株FI3中。
2)燕麦叶枯病菌(Cochliobolus victoriae)菌株FI3转化
a.燕麦叶枯病菌(Cochliobolus victoriae)菌株FI3的产孢培养
从-80℃冰箱取少量燕麦叶枯病菌FI3菌株分生孢子,滴加于CMX培养基【每升CMX培养基包含:0.1g/mL四水合硝酸钙溶液10mL,10mL溶液B,0.5mL微量元素溶液,1g酵母提取物,0.5g酶解干酪素,0.5g酸解干酪素,10g木糖,20g琼脂粉。(每升溶液B包括:20g磷酸二氢钾,25g七水合硫酸镁,15g氯化钠)(每升微量元素溶液包括:57.2mg硼酸,393mg五水合硫酸铜,13.1mg碘化钾,60.4mg一水合硫酸锰,36.8mg四水合钼酸铵,5.49g一水合硫酸锌,948.2mg六水合氯化铁)】上,置24℃培养2周,用CM液体培养基【每升CM培养基包含:0.1g/mL四水合硝酸钙溶液10mL,10mL溶液B,0.5mL微量元素溶液,1g酵母提取物,0.5g酶解干酪素,0.5g酸解干酪素,10g葡萄糖,20g琼脂粉。(每升溶液B包括:20g磷酸二氢钾,25g七水合硫酸镁,15g氯化钠)(每升微量元素溶液包括:57.2mg硼酸,393mg五水合硫酸铜,13.1mg碘化钾,60.4mg一水合硫酸锰,36.8mg四水合钼酸铵,5.49g一水合硫酸锌,948.2mg六水合氯化铁)】刮取、收集孢子,显微镜观察,利用血球计数器调节孢子浓度为1×106/mL。
b.燕麦叶枯病菌(Cochliobolus victoriae)菌株FI3转化
吸取1mL孢子悬浮液于100mLCM液体培养基中,24℃振荡培养(150rpm)28h,离心收集菌丝于80mL酶解液(3.27g氯化钠,0.8g崩溃酶)中酶解2h,收集原生质体。将原生质体用10mLSTC溶液洗涤3次,并最终溶解于500μLSTC溶液(每100mLSTC溶液包括:21.86g山梨醇,1Mtris-HCL1mL,0.735g二水合氯化钙)中。将25mL准备好的PCR片段与100μL原生质体溶液充分混匀,加入1mLPEG溶液(每50mLPEG溶液中包括:聚乙二醇30g,1Mtris-HCL0.5mL,0.37g二水合氯化钙)。最后用1mLSTC溶液稀释,并与再生培养基混合,30℃过夜培养,每个培养皿中加入含有150μg/mL潮霉素的水琼脂10mL,30℃培养3d后挑取扩展的菌落到含有同样抗生素的CMX培养基上。
3)缺失突变体的验证
选用三对引物通过PCR扩增对转化子进行筛选。扩增结果符合如下结果的,确定为CvDUF1基因缺失突变体:上游同源臂之外基因组上的引物U(5'-ACAACGCGTGGATAGAAACA-3')与潮霉素抗性基因的引物NLC37(5'-GGATGCCTCCGCTCGAAGTA-3')配对可以扩增到预期大小(2.1kb)的重组片段;下游同源臂之外基因组上的引物D(5'-CGGTATATCGCCGTTCAACT-3')与潮霉素抗性基因的引物NLC38(5'-CGTTGCAAGACCTGCCTGAA-3')配对可以扩增到预期大小(3.0kb)的重组片段;而编码区引物F(5'-CGGTATGCTCATCGTCCTTT-3')与R(5'-CATTGAGCAGCCTGTCGTAA-3')无扩增条带(野生型菌株可扩增到644kb片段)(见图2)。结果,从转化子中筛选到2株CvDUF1基因缺失突变体,用于后续功能分析。
实施例3CvDUF1基因在燕麦叶枯病菌(Cochliobolus victoriae)菌株FI3毒素产生中
的作用
将燕麦叶枯病菌野生型菌株FI3和CvDUF1基因缺失突变体分别接种于固体CMX培养基上,生长两周后,用5mLFries’培养基【酒石酸铵5g,硝酸铵1g,磷酸二氢钾1g,七水合硫酸镁0.5g,氯化钠0.1g,两水合氯化钙0.13g,蔗糖30g,酵母浸粉1g,铁溶液1mL,用水定容至1L。(铁溶液:七水合硫酸亚铁2g,EDTA2.41g,用水定容至100mL)】冲洗菌丝及分生孢子,收集菌丝及孢子悬浮液至20mLFries’培养基中,黑暗培养15天后用于毒素测定。
毒素生物测定:将上述培养液用纱布过滤,去除菌丝体,取2mL培养液加入试管中,取生长两周左右的燕麦幼苗叶片放入试管中培养24h。过夜培养后如果燕麦叶片保持直立,则证明菌株不能产生毒素,如燕麦叶片变弯曲,则证明菌株能产生毒素。
毒素的仪器测定:将上述培养液用纱布过滤,去除菌丝体,过反向C18柱进行纯化,除去杂质。然后用乙腈/水(50/50)对C18柱进行淋洗,将淋洗液过凝胶柱分离,后用10mMNaCL溶液淋洗,将淋洗液进行浓缩干燥,并用乙腈进行溶解,上高效液相色谱进行测定。高效液相色谱检测条件为:流动相为25%乙腈水溶液,检测器为紫外检测器,检测波长254nm,色谱柱为C18柱。
序 列 表
发明名称:一种燕麦叶枯病菌基因CvDUF1及其应用”
SEQ ID No:1的序列
(i)序列特征:(A)长度:1004 bp;(B)类型:核苷酸;(C)链性:单链
(ii)分子类型:DNA
(iii)序列描述:SEQ ID No:1
1-60 atgagctcag cagcatatag ccgagtagat tcaagttcaa ctgacagaaatagtgatgaa
61-120 tatcacaaag aacggtacct tgcacttaga acgaataatg ctaagggtttcaaaggagtt 121-180 caaaggcgat tatggatagc gattaattcc agtttatggc ttgttacactcggtatgctc 181-240 atcgtccttt tacggcggga gagtttgggc acgagaaacg gcgggtttgagcttgggttc 241-300 tcagctactg agcttggtat gacaaacaac attgatctac cgtattcatccaatcgctaa 301-360 ctagaaaatc agccgctacg tttcgaacca ttagaatctc tactgagatattcaatcagc 361-420 ctgaaggaac atcgttctct acattaccta cgtcgtctta tgtaggtgaaccaacagagg 421-480 agattgacaa tgcttggagg atattagttt cccgtaagca agactttccagaatcaatta 481-540 ctcaggccta tgctcattgc tcttctcact agcaactgtc ctttacctaaaagaggacga 541-600 gattggcgag tataaaaaca gcactaccaa gcaagacaac ctctgggtcgccgggtcagt 601-660 tttataccaa acttggctga acctcatgcc ttatttctag taaaaatagacgttgctaac 661-720 attttcattt ggttagtttg caagtatacc actaccttca ctgcattaacgcactacgcc 721-780 gcgcagcata ccaacatgtt tacggggctc cgacaaagga acatctaaatcacctcgacc 781-840 attgcattga catgttacga caggctgctc aatgtcaatc tgacctgacacctatgttat 841-900 atttcaaccc cgagaatgac ccaaacacta tgctcattaa gtcacaccaacactcttgtc 901-960 gccggtttga tttagtaaat gaatgggcga tggctcggtc tcagtgcaaagggaatacta 961-1004cttgtgccat cgaagtaggg aagcaagtag gcggcgagat gtaa
Claims (1)
1.一种燕麦叶枯病菌(Cochliobolus victoriae)CvDUF1基因作为靶标在设计和筛选抗燕麦叶枯病药剂中应用,其中所述基因的DNA序列如SEQ ID NO.1所示。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910950510.8A CN110592107B (zh) | 2019-10-08 | 2019-10-08 | 一种燕麦叶枯病菌基因CvDUF1及其应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910950510.8A CN110592107B (zh) | 2019-10-08 | 2019-10-08 | 一种燕麦叶枯病菌基因CvDUF1及其应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN110592107A CN110592107A (zh) | 2019-12-20 |
CN110592107B true CN110592107B (zh) | 2021-09-17 |
Family
ID=68865728
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201910950510.8A Active CN110592107B (zh) | 2019-10-08 | 2019-10-08 | 一种燕麦叶枯病菌基因CvDUF1及其应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN110592107B (zh) |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2015201677A1 (en) * | 2011-06-13 | 2015-04-23 | Suncor Energy Inc. | Delivery of paraffinic oil-containing compositions to root tissue of plants |
US9573980B2 (en) * | 2013-03-15 | 2017-02-21 | Spogen Biotech Inc. | Fusion proteins and methods for stimulating plant growth, protecting plants from pathogens, and immobilizing Bacillus spores on plant roots |
-
2019
- 2019-10-08 CN CN201910950510.8A patent/CN110592107B/zh active Active
Also Published As
Publication number | Publication date |
---|---|
CN110592107A (zh) | 2019-12-20 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Proctor et al. | Fumonisin production in the maize pathogen Fusarium verticillioides: genetic basis of naturally occurring chemical variation | |
Deng et al. | Genome editing in Shiraia bambusicola using CRISPR-Cas9 system | |
Hof et al. | Ferricrocin synthesis in Magnaporthe grisea and its role in pathogenicity in rice | |
Liu et al. | Agrobacterium-mediated transformation as a useful tool for the molecular genetic study of the phytopathogen Curvularia lunata | |
Li et al. | Siderophore biosynthesis but not reductive iron assimilation is essential for the dimorphic fungus Nomuraea rileyi conidiation, dimorphism transition, resistance to oxidative stress, pigmented microsclerotium formation, and virulence | |
CN105154454B (zh) | 一种与致病力相关的灰霉病菌基因BcArs2及其应用 | |
CN109609526B (zh) | 一种大麦条纹病致病性基因PgPBS及其应用 | |
Ushimaru et al. | Development of an efficient gene targeting system in Colletotrichum higginsianum using a non-homologous end-joining mutant and Agrobacterium tumefaciens-mediated gene transfer | |
El Hadrami et al. | A cupin domain-containing protein with a quercetinase activity (VdQase) regulates Verticillium dahliae's pathogenicity and contributes to counteracting host defenses | |
CN105154453A (zh) | 一种与致病力相关的灰霉病菌基因BcSep4及其应用 | |
Reilly et al. | Forward genetics screen coupled with whole-genome resequencing identifies novel gene targets for improving heterologous enzyme production in Aspergillus niger | |
Xie et al. | Characterization of three multicopper oxidases in the filamentous fungus Podospora anserina: A new role of an ABR1-like protein in fungal development? | |
Zhang et al. | Functional analysis of the pathogenicity-related gene VdPR1 in the vascular wilt fungus Verticillium dahliae | |
Zhu et al. | Overexpression of chitinase in the endophyte Phomopsis liquidambaris enhances wheat resistance to Fusarium graminearum | |
Binh et al. | Establishment of a new and efficient Agrobacterium-mediated transformation system in the nematicidal fungus Purpureocillium lilacinum | |
L’Haridon et al. | Isolation of differentially expressed genes during interactions between tomato cells and a protective or a non-protective strain of Fusarium oxysporum | |
CN110592107B (zh) | 一种燕麦叶枯病菌基因CvDUF1及其应用 | |
Ito et al. | Post‐transcriptional silencing of the tomatinase gene in Fusarium oxysporum f. sp lycopersici | |
CN105483146A (zh) | 一种与致病力相关的灰霉病菌基因BcAls1及其应用 | |
CN110846328B (zh) | 一种燕麦叶枯病菌基因CvCAO1及其应用 | |
CN108410879B (zh) | 水稻捕光色素叶绿素a/b结合蛋白Lhcb5在稻瘟病菌抗性中的应用 | |
Callejas-Negrete et al. | The Adh1 gene of the fungus Metarhizium anisopliae is expressed during insect colonization and required for full virulence | |
CN110257402A (zh) | 一种玉米小斑病菌ChCDC10基因及其应用 | |
Santhanam et al. | A reassessment of flocculosin-mediated biocontrol activity of Pseudozyma flocculosa through CRISPR/Cas9 gene editing | |
CN107043409A (zh) | 大丽轮枝菌产微菌核相关基因VdGAP的功能分析及其应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |